LOCUS       DADLDT020000001        78784 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000001 DADLDT020000000
VERSION     DADLDT020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.001"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..228
                     /locus_tag="JQH22_000001"
     CDS             <1..228
                     /locus_tag="JQH22_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFB2204475.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="JQH22_000002"
                     /pseudo
     CDS             648..1263
                     /locus_tag="JQH22_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="JQH22_000003"
     CDS             1366..1689
                     /locus_tag="JQH22_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFB2204476.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="JQH22_000004"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="JQH22_000004"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFB2204477.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="JQH22_000005"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="JQH22_000005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFB2204478.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="JQH22_000006"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="JQH22_000006"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFB2204479.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="JQH22_000007"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="JQH22_000007"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFB2204480.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="JQH22_000008"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="JQH22_000008"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFB2204481.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="JQH22_000009"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="JQH22_000009"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFB2204482.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="JQH22_000010"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="JQH22_000010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFB2204483.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="JQH22_000011"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="JQH22_000011"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFB2204484.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="JQH22_000012"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="JQH22_000012"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFB2204485.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="JQH22_000013"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="JQH22_000013"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFB2204486.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="JQH22_000014"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="JQH22_000014"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFB2204487.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="JQH22_000015"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="JQH22_000015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFB2204488.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="JQH22_000016"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="JQH22_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFB2204489.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="JQH22_000017"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="JQH22_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFB2204490.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="JQH22_000018"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="JQH22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFB2204491.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="JQH22_000019"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="JQH22_000019"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFB2204492.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="JQH22_000020"
     CDS             complement(21228..23060)
                     /locus_tag="JQH22_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFB2204493.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="JQH22_000021"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="JQH22_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFB2204494.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="JQH22_000022"
     CDS             complement(23808..24302)
                     /locus_tag="JQH22_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFB2204495.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="JQH22_000023"
     CDS             complement(24385..24840)
                     /locus_tag="JQH22_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HFB2204496.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="JQH22_000024"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="JQH22_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFB2204497.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="JQH22_000025"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="JQH22_000025"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFB2204498.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="JQH22_000026"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="JQH22_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFB2204499.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="JQH22_000027"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="JQH22_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFB2204500.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="JQH22_000028"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="JQH22_000028"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFB2204501.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="JQH22_000029"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="JQH22_000029"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFB2204502.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="JQH22_000030"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="JQH22_000030"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFB2204503.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="JQH22_000031"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="JQH22_000031"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFB2204504.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="JQH22_000032"
     CDS             33416..34309
                     /locus_tag="JQH22_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFB2204505.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="JQH22_000033"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="JQH22_000033"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFB2204506.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="JQH22_000034"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="JQH22_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFB2204507.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="JQH22_000035"
     CDS             complement(36225..36941)
                     /locus_tag="JQH22_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2204508.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="JQH22_000036"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="JQH22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFB2204509.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="JQH22_000037"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="JQH22_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFB2204510.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="JQH22_000038"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="JQH22_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFB2204511.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="JQH22_000039"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="JQH22_000039"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFB2204512.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="JQH22_000040"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="JQH22_000040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFB2204513.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="JQH22_000041"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="JQH22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFB2204514.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="JQH22_000042"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="JQH22_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFB2204515.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="JQH22_000043"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="JQH22_000043"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFB2204516.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="JQH22_000044"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="JQH22_000044"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFB2204517.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="JQH22_000045"
     CDS             complement(45790..46449)
                     /locus_tag="JQH22_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2204518.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="JQH22_000046"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="JQH22_000046"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFB2204519.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="JQH22_000047"
     CDS             complement(47344..48357)
                     /locus_tag="JQH22_000047"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFB2204520.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="JQH22_000048"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="JQH22_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFB2204521.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="JQH22_000049"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="JQH22_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFB2204522.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="JQH22_000050"
     CDS             complement(50650..51828)
                     /locus_tag="JQH22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2204523.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="JQH22_000051"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="JQH22_000051"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFB2204524.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="JQH22_000052"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="JQH22_000052"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFB2204525.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="JQH22_000053"
     CDS             complement(55610..55888)
                     /locus_tag="JQH22_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFB2204526.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="JQH22_000054"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="JQH22_000054"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFB2204527.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="JQH22_000055"
     CDS             complement(56470..57279)
                     /locus_tag="JQH22_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFB2204528.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="JQH22_000056"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="JQH22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFB2204529.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="JQH22_000057"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="JQH22_000057"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFB2204530.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="JQH22_000058"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="JQH22_000058"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFB2204531.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="JQH22_000059"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="JQH22_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFB2204532.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="JQH22_000060"
     CDS             complement(60980..61870)
                     /locus_tag="JQH22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HFB2204533.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="JQH22_000061"
     CDS             complement(61857..62381)
                     /locus_tag="JQH22_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2204534.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="JQH22_000062"
     CDS             complement(62378..62848)
                     /locus_tag="JQH22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2204535.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="JQH22_000063"
     CDS             complement(62845..63393)
                     /locus_tag="JQH22_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2204536.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="JQH22_000064"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="JQH22_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="JQH22_000065"
     CDS             complement(64140..64862)
                     /locus_tag="JQH22_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFB2204537.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="JQH22_000066"
     CDS             complement(64922..66154)
                     /locus_tag="JQH22_000066"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFB2204538.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="JQH22_000067"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="JQH22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67426)
                     /locus_tag="JQH22_000068"
     CDS             complement(66863..67426)
                     /locus_tag="JQH22_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFB2204539.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="JQH22_000069"
     CDS             complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="JQH22_000069"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFB2204540.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="JQH22_000070"
                     /pseudo
     CDS             complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="JQH22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="JQH22_000071"
     CDS             complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="JQH22_000071"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFB2204541.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72432..72716)
                     /locus_tag="JQH22_000072"
     CDS             complement(72432..72716)
                     /locus_tag="JQH22_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFB2204542.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73088..74428
                     /gene="fadL"
                     /locus_tag="JQH22_000073"
     CDS             73088..74428
                     /gene="fadL"
                     /locus_tag="JQH22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFB2204543.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75179..75853
                     /locus_tag="JQH22_000074"
     CDS             75179..75853
                     /locus_tag="JQH22_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204544.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="JQH22_000075"
     CDS             complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="JQH22_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFB2204545.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77084..78016
                     /locus_tag="JQH22_000076"
     CDS             77084..78016
                     /locus_tag="JQH22_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFB2204546.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78090..78164
                     /locus_tag="JQH22_000077"
     tRNA            78090..78164
                     /locus_tag="JQH22_000077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78123..78125,aa:Arg,seq:cct)
     gene            78326..>78784
                     /gene="intS"
                     /locus_tag="JQH22_000078"
     CDS             78326..>78784
                     /gene="intS"
                     /locus_tag="JQH22_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFB2204547.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19966 a        21040 c        19326 g        18452 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcgccgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt
    67501 atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt
    67561 cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa
    67621 agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct
    67681 taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac
    67741 agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccact tttgacaata
    67801 taatatataa acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat
    67861 tcaactctat tttctctatt atctgtatta ataaatgaca atgagtgtca aacagatatt
    67921 attcatttca acatgtaact ttgggaaaaa tcaagatacg cgcagaagaa taatttccga
    67981 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
    68041 cagatatccg ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt
    68101 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
    68161 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
    68221 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
    68281 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
    68341 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
    68401 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
    68461 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
    68521 agtggtcaga ggacgaaccg aatcactggc ggaatcgagg gctgcgtcgc cgtgacgcat
    68581 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
    68641 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
    68701 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
    68761 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
    68821 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
    68881 gggaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
    68941 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
    69001 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
    69061 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcac ccctgccctt
    69121 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
    69181 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
    69241 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
    69301 taattttagt cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa
    69361 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
    69421 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
    69481 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
    69541 gcgccgatgt acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga
    69601 agaaatgcag gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
    69661 gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg
    69721 ccaccatctg ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
    69781 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
    69841 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
    69901 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
    69961 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
    70021 atgccgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
    70081 tactacgcaa agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag
    70141 cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
    70201 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
    70261 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
    70321 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
    70381 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
    70441 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
    70501 cgggtaacaa tccaagttgt acttcagaca aaccgagcac cgttttagga tcgtcagtac
    70561 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
    70621 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
    70681 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc
    70741 cggcgataaa attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt
    70801 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca
    70861 ttttctcatc cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag
    70921 taaacgctga tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
    70981 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
    71041 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc
    71101 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
    71161 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc
    71221 ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag
    71281 cggtgttgac caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
    71341 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat
    71401 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
    71461 gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
    71521 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
    71581 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
    71641 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
    71701 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
    71761 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
    71821 cagcacgtgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
    71881 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
    71941 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
    72001 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
    72061 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
    72121 caccatcttc cctaaatcaa ccgtgggaat gccatgaaaa gccgtcgcct ggcgggcaaa
    72181 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa
    72241 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
    72301 tcttaaccat ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac
    72361 agtgaagaca gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    72421 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    72481 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
    72541 cacggttcgg attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct
    72601 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    72661 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    72721 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    72781 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    72841 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    72901 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
    72961 tagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    73021 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    73081 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    73141 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    73201 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    73261 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    73321 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    73381 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    73441 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
    73501 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    73561 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc
    73621 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    73681 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    73741 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    73801 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    73861 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    73921 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    73981 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    74041 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    74101 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    74161 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    74221 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    74281 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    74341 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    74401 cggtactaac tttaactacg cgttctgata acgcgttcgc ctagataaag tcacctgcta
    74461 tgcaggtgac tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg
    74521 agttttgcaa acagcctgat ggcagcttgc cctttcaaat cagtaagtag tatccacaga
    74581 caaacctgaa gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac
    74641 ttttctgcat ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt
    74701 aaagataggt tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata
    74761 tttgtaacaa atcaatcaac atggaataaa atcatgctac catctatttc aatcaacaat
    74821 accagcgcag cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta
    74881 gtacaagagt tcaaaaacca tttttaatgg taaggaagga ataagcacct gtattaaaca
    74941 tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata gatctaatat
    75001 taataattcc tcagttactt atattgatat tggctccaat gatacagacc atattactat
    75061 tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt
    75121 ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat
    75181 gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct tcgataagat
    75241 atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaaaa gcgaaccaac
    75301 ggacttattt acctggtacg gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa
    75361 aaataaaaac ggaaatgatt acttatttct cggttactac gatcaggacg actaccacat
    75421 tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg aaaacgccag
    75481 gagtgcttca gcgtggtttg aaagcagtaa agctatcatg gcagaatcat ttgcaactgg
    75541 ttccgatcat caggttgtaa acgagctcaa cggggaaaga ctgagagaac ctaacgaagt
    75601 ttttaaacgt ttaggtcgag caataagata taattttcaa gtggacgatg caaaatttag
    75661 acgcgataat gtaaaagaaa taatttctac tttattcgct aacaaagttg acgttgacca
    75721 tcctgaaaat aaatacaaag actttaagaa tctcgaagat aaagttgaaa aaagacttca
    75781 aaatcgccag acgaaatatc aaaatgaaat taatcaacta tctgcactag gtgttaattt
    75841 tgatgatatt taaaattatt tttccagttt tttgattttt caacgcctga atatcggaaa
    75901 tagtcagtga caaacatcag tatttttaca tcattacgtc cgggtcattg tcggtttatc
    75961 tccttttact tgtggtaatt gagggataac aacaaaaaag gtgagttttg cgactcacct
    76021 tttttatttg tttcttattc agaatcgata tcttttaaat catcctgaat cgcttgcgcg
    76081 ttcggatttt cctgcggttt gagttcgccg ccattagcga tgaaatcatg acgctggaag
    76141 tacgcttcgc gcaccataat ataaggatcg gacgactgac gcagcagacc atcggaatcc
    76201 agcagctgag cgcgggtttc gatcccttca agcgtccatt tacccacaga catcggccag
    76261 gtcagccagg aaagaaccgg gtaaagagca tccgccatat caccaccgtc atcacgcagc
    76321 gtgaagctac cgtagaacgg taactgaacg taaggcccat aacccacgcc ataatgacca
    76381 agcgtactac cgaagcggtg aggttcagtc cgttgcagtt tcgggttcgc catccctgca
    76441 acatcaataa agccgcccat ccccaaaatg gtgttcagga aaaagcgggt aaagtggacc
    76501 atcccctgat aagggtcgcc ctgcaagaag tagttaacca tcaccgcagg ttcttcaagg
    76561 ttgccagtaa agttgctcaa accattacgc gccggttgcg gaacataatc acgccaggcg
    76621 acagcgaccg gtcgaacaat atacgggtct aatacgttga agttgaagtt gtacatggtg
    76681 cggttgaacc cttctaacgg gtcagaacgc ccttgctgat ctgtaccgga actcgcacac
    76741 cccaccagaa gcgtagttcc cagagcaagc gccgacaggc gaagcttcat aaatgtctcc
    76801 ctgttttttt atggcttatg cagtttgcca tccatgacgg aacgataccg tatccgcctg
    76861 tttaggtgtg ggcgattgta acagcacgtc aactgatgtc cagacgccct gatttgctga
    76921 tttgatcata gcctggtaat cgccgccctg taggctactt gattctatag aaacagaaaa
    76981 aggcaaacgc agccttttct acattttcag agtaactccc gccagttgcg agcaaaaaag
    77041 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
    77101 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
    77161 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
    77221 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
    77281 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgccg aactggaagg
    77341 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
    77401 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
    77461 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
    77521 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
    77581 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
    77641 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
    77701 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
    77761 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
    77821 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
    77881 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
    77941 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
    78001 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
    78061 agtccattag cgggtatact cgccgcattg tcctcttagt taaatggata taacgagccc
    78121 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accatttatc agttcgctcc
    78181 catccgtacc agtccgcaaa atcccctgaa tatcaagcct tccgtatatt cacagttcgt
    78241 catggttcgc gtcagatcgt tgacagccgc actccatgac gggtaaaaag tggataaaat
    78301 aattttaccc accggatttt tacccatgct caccgttaag cagattgaag cagcaaagcc
    78361 gaaagaaaaa ccataccgcc ttctcgatgg taatggcctg tacctttatg tccctgtatc
    78421 cgggaaaaag gtatggcagc ttcgctacaa gattgacggt aaggagaaaa tcctgaccgt
    78481 cggaaaatat ccgcttatga ctttgcagga ggcaagggat aaagcatgga ctgcgaggaa
    78541 agacatctcg gctggcatcg atccggtaaa ggcgaaaaag gcttcgtcta acaacaattc
    78601 atttagtgcg atttacaagg aatggtacga acacaagaag caagtctggt cagtagggta
    78661 tgcgactgaa cttgcaaaaa tgtttgatga cgacatttta cccatcatcg gcggccttga
    78721 gattcaggat attgagccga tgcaactgct ggaagtaatc cgcagatttg aagatcgcgg
    78781 tgca
//
LOCUS       DADLDT020000002        75873 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000002 DADLDT020000000
VERSION     DADLDT020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 75873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75873
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.002"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..208)
                     /locus_tag="JQH22_000079"
     CDS             complement(<1..208)
                     /locus_tag="JQH22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204548.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(210..430)
                     /locus_tag="JQH22_000080"
                     /pseudo
     CDS             complement(210..430)
                     /locus_tag="JQH22_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(423..644)
                     /locus_tag="JQH22_000081"
     CDS             complement(423..644)
                     /locus_tag="JQH22_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204549.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(634..834)
                     /locus_tag="JQH22_000082"
     CDS             complement(634..834)
                     /locus_tag="JQH22_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFB2204550.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            964..1143
                     /locus_tag="JQH22_000083"
     CDS             964..1143
                     /locus_tag="JQH22_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204551.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1268..1597
                     /locus_tag="JQH22_000084"
     CDS             1268..1597
                     /locus_tag="JQH22_000084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204552.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1647..1835)
                     /locus_tag="JQH22_000085"
     CDS             complement(1647..1835)
                     /locus_tag="JQH22_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HFB2204553.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2210..3438)
                     /locus_tag="JQH22_000086"
                     /pseudo
     CDS             complement(2210..3438)
                     /locus_tag="JQH22_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3687..4808
                     /gene="roxA"
                     /locus_tag="JQH22_000087"
                     /gene_synonym="ycfD"
     CDS             3687..4808
                     /gene="roxA"
                     /locus_tag="JQH22_000087"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFB2204554.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="JQH22_000088"
     CDS             complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="JQH22_000088"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFB2204555.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6333..7469
                     /gene="potA"
                     /locus_tag="JQH22_000089"
     CDS             6333..7469
                     /gene="potA"
                     /locus_tag="JQH22_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFB2204556.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7453..8310
                     /gene="potB"
                     /locus_tag="JQH22_000090"
     CDS             7453..8310
                     /gene="potB"
                     /locus_tag="JQH22_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFB2204557.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8307..9101
                     /gene="potC"
                     /locus_tag="JQH22_000091"
     CDS             8307..9101
                     /gene="potC"
                     /locus_tag="JQH22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFB2204558.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9098..10144
                     /gene="potD"
                     /locus_tag="JQH22_000092"
     CDS             9098..10144
                     /gene="potD"
                     /locus_tag="JQH22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFB2204559.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="JQH22_000093"
     CDS             complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="JQH22_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFB2204560.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="JQH22_000094"
     CDS             complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="JQH22_000094"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFB2204561.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="JQH22_000095"
     CDS             complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="JQH22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFB2204562.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="JQH22_000096"
     CDS             complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="JQH22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFB2204563.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="JQH22_000097"
     CDS             complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="JQH22_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFB2204564.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15476..16549
                     /locus_tag="JQH22_000098"
     CDS             15476..16549
                     /locus_tag="JQH22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFB2204565.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16677..20123
                     /gene="mfd"
                     /locus_tag="JQH22_000099"
     CDS             16677..20123
                     /gene="mfd"
                     /locus_tag="JQH22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFB2204566.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20266..21228
                     /gene="ldtC"
                     /locus_tag="JQH22_000100"
     CDS             20266..21228
                     /gene="ldtC"
                     /locus_tag="JQH22_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFB2204567.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21311..21523)
                     /locus_tag="JQH22_000101"
     CDS             complement(21311..21523)
                     /locus_tag="JQH22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFB2204568.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21808..22440
                     /gene="comR"
                     /locus_tag="JQH22_000102"
     CDS             21808..22440
                     /gene="comR"
                     /locus_tag="JQH22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFB2204569.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22502..23041)
                     /locus_tag="JQH22_000103"
     CDS             complement(22502..23041)
                     /locus_tag="JQH22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFB2204570.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="JQH22_000104"
     CDS             complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="JQH22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFB2204571.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24980..25522)
                     /locus_tag="JQH22_000105"
     CDS             complement(24980..25522)
                     /locus_tag="JQH22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFB2204572.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="JQH22_000106"
     CDS             complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="JQH22_000106"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFB2204573.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="JQH22_000107"
     CDS             complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="JQH22_000107"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFB2204574.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="JQH22_000108"
     CDS             complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="JQH22_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFB2204575.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28042..28419)
                     /locus_tag="JQH22_000109"
     CDS             complement(28042..28419)
                     /locus_tag="JQH22_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFB2204576.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="JQH22_000110"
     CDS             complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="JQH22_000110"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFB2204577.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            28912..29093
                     /locus_tag="JQH22_000111"
                     /pseudo
     CDS             28912..29093
                     /locus_tag="JQH22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29126..31311
                     /gene="fhuE"
                     /locus_tag="JQH22_000112"
                     /pseudo
     CDS             29126..31311
                     /gene="fhuE"
                     /locus_tag="JQH22_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31371..32804)
                     /gene="ptsG"
                     /locus_tag="JQH22_000113"
     CDS             complement(31371..32804)
                     /gene="ptsG"
                     /locus_tag="JQH22_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFB2204578.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33099..33896)
                     /locus_tag="JQH22_000114"
     CDS             complement(33099..33896)
                     /locus_tag="JQH22_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFB2204579.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33907..34911)
                     /gene="holB"
                     /locus_tag="JQH22_000115"
     CDS             complement(33907..34911)
                     /gene="holB"
                     /locus_tag="JQH22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFB2204580.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34908..35549)
                     /locus_tag="JQH22_000116"
     CDS             complement(34908..35549)
                     /locus_tag="JQH22_000116"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFB2204581.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35539..36561)
                     /gene="yceG"
                     /locus_tag="JQH22_000117"
     CDS             complement(35539..36561)
                     /gene="yceG"
                     /locus_tag="JQH22_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFB2204582.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36564..37373)
                     /gene="pabC"
                     /locus_tag="JQH22_000118"
     CDS             complement(36564..37373)
                     /gene="pabC"
                     /locus_tag="JQH22_000118"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFB2204583.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37493..38734)
                     /gene="fabF"
                     /locus_tag="JQH22_000119"
     CDS             complement(37493..38734)
                     /gene="fabF"
                     /locus_tag="JQH22_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFB2204584.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38821..39057)
                     /gene="acpP"
                     /locus_tag="JQH22_000120"
     CDS             complement(38821..39057)
                     /gene="acpP"
                     /locus_tag="JQH22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFB2204585.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39268..40002)
                     /gene="fabG"
                     /locus_tag="JQH22_000121"
     CDS             complement(39268..40002)
                     /gene="fabG"
                     /locus_tag="JQH22_000121"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFB2204586.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40015..40944)
                     /gene="fabD"
                     /locus_tag="JQH22_000122"
     CDS             complement(40015..40944)
                     /gene="fabD"
                     /locus_tag="JQH22_000122"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFB2204587.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40960..41913)
                     /gene="fabH"
                     /locus_tag="JQH22_000123"
     CDS             complement(40960..41913)
                     /gene="fabH"
                     /locus_tag="JQH22_000123"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFB2204588.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41981..43051)
                     /gene="plsX"
                     /locus_tag="JQH22_000124"
     CDS             complement(41981..43051)
                     /gene="plsX"
                     /locus_tag="JQH22_000124"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFB2204589.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43132..43305)
                     /gene="rpmF"
                     /locus_tag="JQH22_000125"
     CDS             complement(43132..43305)
                     /gene="rpmF"
                     /locus_tag="JQH22_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFB2204590.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43357..43878)
                     /gene="yceD"
                     /locus_tag="JQH22_000126"
     CDS             complement(43357..43878)
                     /gene="yceD"
                     /locus_tag="JQH22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFB2204591.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44077..44661
                     /gene="yceF"
                     /locus_tag="JQH22_000127"
     CDS             44077..44661
                     /gene="yceF"
                     /locus_tag="JQH22_000127"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFB2204592.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44773..45732)
                     /gene="rluC"
                     /locus_tag="JQH22_000128"
     CDS             complement(44773..45732)
                     /gene="rluC"
                     /locus_tag="JQH22_000128"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFB2204593.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46305..49490
                     /gene="rne"
                     /locus_tag="JQH22_000129"
     CDS             46305..49490
                     /gene="rne"
                     /locus_tag="JQH22_000129"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFB2204594.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49686..50639)
                     /gene="flgL"
                     /locus_tag="JQH22_000130"
     CDS             complement(49686..50639)
                     /gene="flgL"
                     /locus_tag="JQH22_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFB2204595.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50651..52293)
                     /gene="flgK"
                     /locus_tag="JQH22_000131"
                     /pseudo
     CDS             complement(50651..52293)
                     /gene="flgK"
                     /locus_tag="JQH22_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52359..53300)
                     /gene="flgJ"
                     /locus_tag="JQH22_000132"
     CDS             complement(52359..53300)
                     /gene="flgJ"
                     /locus_tag="JQH22_000132"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFB2204596.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53300..54397)
                     /gene="flgI"
                     /locus_tag="JQH22_000133"
     CDS             complement(53300..54397)
                     /gene="flgI"
                     /locus_tag="JQH22_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFB2204597.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54409..55107)
                     /gene="flgH"
                     /locus_tag="JQH22_000134"
     CDS             complement(54409..55107)
                     /gene="flgH"
                     /locus_tag="JQH22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFB2204598.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNSN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55160..55942)
                     /gene="flgG"
                     /locus_tag="JQH22_000135"
     CDS             complement(55160..55942)
                     /gene="flgG"
                     /locus_tag="JQH22_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFB2204599.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56080..56831)
                     /gene="flgF"
                     /locus_tag="JQH22_000136"
                     /pseudo
     CDS             complement(56080..56831)
                     /gene="flgF"
                     /locus_tag="JQH22_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56851..58059)
                     /gene="flgE"
                     /locus_tag="JQH22_000137"
     CDS             complement(56851..58059)
                     /gene="flgE"
                     /locus_tag="JQH22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFB2204600.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58084..58779)
                     /gene="flgD"
                     /locus_tag="JQH22_000138"
                     /pseudo
     CDS             complement(58084..58779)
                     /gene="flgD"
                     /locus_tag="JQH22_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(58791..59194)
                     /gene="flgC"
                     /locus_tag="JQH22_000139"
                     /pseudo
     CDS             complement(58791..59194)
                     /gene="flgC"
                     /locus_tag="JQH22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59198..59614)
                     /gene="flgB"
                     /locus_tag="JQH22_000140"
     CDS             complement(59198..59614)
                     /gene="flgB"
                     /locus_tag="JQH22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFB2204601.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59769..60428
                     /gene="flgA"
                     /locus_tag="JQH22_000141"
     CDS             59769..60428
                     /gene="flgA"
                     /locus_tag="JQH22_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HFB2204602.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60504..60797
                     /gene="flgM"
                     /locus_tag="JQH22_000142"
     CDS             60504..60797
                     /gene="flgM"
                     /locus_tag="JQH22_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HFB2204603.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60802..61218
                     /gene="flgN"
                     /locus_tag="JQH22_000143"
     CDS             60802..61218
                     /gene="flgN"
                     /locus_tag="JQH22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFB2204604.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61258..62793)
                     /gene="murJ"
                     /locus_tag="JQH22_000144"
     CDS             complement(61258..62793)
                     /gene="murJ"
                     /locus_tag="JQH22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFB2204605.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62960..63826)
                     /locus_tag="JQH22_000145"
     CDS             complement(62960..63826)
                     /locus_tag="JQH22_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFB2204606.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63828..64475)
                     /locus_tag="JQH22_000146"
                     /pseudo
     CDS             complement(63828..64475)
                     /locus_tag="JQH22_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64486..65070)
                     /gene="rimJ"
                     /locus_tag="JQH22_000147"
     CDS             complement(64486..65070)
                     /gene="rimJ"
                     /locus_tag="JQH22_000147"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFB2204607.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65306..66514
                     /gene="mdtH"
                     /locus_tag="JQH22_000148"
     CDS             65306..66514
                     /gene="mdtH"
                     /locus_tag="JQH22_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFB2204608.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66578..67225
                     /gene="grxB"
                     /locus_tag="JQH22_000149"
     CDS             66578..67225
                     /gene="grxB"
                     /locus_tag="JQH22_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFB2204609.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67359..67919
                     /locus_tag="JQH22_000150"
     CDS             67359..67919
                     /locus_tag="JQH22_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFB2204610.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68025..69071
                     /gene="pyrC"
                     /locus_tag="JQH22_000151"
     CDS             68025..69071
                     /gene="pyrC"
                     /locus_tag="JQH22_000151"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFB2204611.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69145..69390
                     /gene="dinI"
                     /locus_tag="JQH22_000152"
     CDS             69145..69390
                     /gene="dinI"
                     /locus_tag="JQH22_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFB2204612.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69680..69934
                     /gene="bssS"
                     /locus_tag="JQH22_000153"
     CDS             69680..69934
                     /gene="bssS"
                     /locus_tag="JQH22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFB2204613.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70049..71167
                     /gene="solA"
                     /locus_tag="JQH22_000154"
     CDS             70049..71167
                     /gene="solA"
                     /locus_tag="JQH22_000154"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFB2204614.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71215..71328
                     /locus_tag="JQH22_000155"
     CDS             71215..71328
                     /locus_tag="JQH22_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFB2204615.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71589..72155
                     /locus_tag="JQH22_000156"
     CDS             71589..72155
                     /locus_tag="JQH22_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFB2204616.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72159..72734
                     /locus_tag="JQH22_000157"
     CDS             72159..72734
                     /locus_tag="JQH22_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFB2204617.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72776..73828)
                     /locus_tag="JQH22_000158"
     CDS             complement(72776..73828)
                     /locus_tag="JQH22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFB2204618.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74053..74973
                     /gene="lpxL"
                     /locus_tag="JQH22_000159"
     CDS             74053..74973
                     /gene="lpxL"
                     /locus_tag="JQH22_000159"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFB2204619.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75145..75342
                     /locus_tag="JQH22_000160"
     CDS             75145..75342
                     /locus_tag="JQH22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFB2204620.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75366..>75873
                     /locus_tag="JQH22_000161"
     CDS             75366..>75873
                     /locus_tag="JQH22_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2204621.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKV"
BASE COUNT        18496 a        20067 c        18765 g        18545 t
ORIGIN      
        1 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
       61 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
      121 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
      181 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
      241 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
      301 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
      361 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
      421 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
      481 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
      541 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
      601 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
      661 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
      721 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
      781 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
      841 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
      901 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
      961 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
     1021 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
     1081 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
     1141 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
     1201 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
     1261 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
     1321 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
     1381 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
     1441 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
     1501 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
     1561 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
     1621 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
     1681 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
     1741 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
     1801 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
     1861 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
     1921 ggcaaatgtt gaggccgcat ggtttacaga aagaggaata atcaggataa aatcctttaa
     1981 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
     2041 agtgattcgc tagtgtataa aaaatcagca acacattaaa aatcagctat ttataaatct
     2101 gtacactttt tcacctcttg ttcgtagttg ttccgctttg ttgctcattg ttgcacgttg
     2161 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
     2221 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
     2281 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
     2341 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
     2401 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
     2461 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
     2521 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
     2581 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
     2641 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
     2701 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
     2761 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
     2821 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
     2881 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
     2941 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
     3001 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
     3061 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
     3121 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt tcccctctgc
     3181 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggttca gatcaggata
     3241 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
     3301 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
     3361 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
     3421 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
     3481 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
     3541 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
     3601 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
     3661 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
     3721 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
     3781 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
     3841 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
     3901 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
     3961 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
     4021 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
     4081 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
     4141 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
     4201 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
     4261 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
     4321 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
     4381 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
     4441 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
     4501 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
     4561 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
     4621 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
     4681 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
     4741 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
     4801 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
     4861 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
     4921 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
     4981 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
     5041 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
     5101 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
     5161 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
     5221 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
     5281 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
     5341 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
     5401 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
     5461 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
     5521 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
     5581 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
     5641 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
     5701 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
     5761 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
     5821 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
     5881 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
     5941 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
     6001 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
     6061 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
     6121 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
     6181 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
     6241 aagcggtttg ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa
     6301 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
     6361 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
     6421 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
     6481 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
     6541 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
     6601 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
     6661 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
     6721 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
     6781 gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt
     6841 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
     6901 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
     6961 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
     7021 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
     7081 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
     7141 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
     7201 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
     7261 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
     7321 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
     7381 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
     7441 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
     7501 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
     7561 acccgcgacg acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg
     7621 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
     7681 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
     7741 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
     7801 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
     7861 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
     7921 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
     7981 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
     8041 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
     8101 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
     8161 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
     8221 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
     8281 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
     8341 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
     8401 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
     8461 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
     8521 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
     8581 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
     8641 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
     8701 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
     8761 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
     8821 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
     8881 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
     8941 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
     9001 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
     9061 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
     9121 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
     9181 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
     9241 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
     9301 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
     9361 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
     9421 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
     9481 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
     9541 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
     9601 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
     9661 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
     9721 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
     9781 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
     9841 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
     9901 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
     9961 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    10021 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    10081 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    10141 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    10201 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    10261 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    10321 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    10381 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    10441 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    10501 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    10561 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    10621 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    10681 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    10741 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    10801 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    10861 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    10921 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    10981 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    11041 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    11101 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    11161 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc
    11221 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    11281 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    11341 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    11401 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    11461 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    11521 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    11581 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    11641 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    11701 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    11761 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    11821 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    11881 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    11941 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    12001 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    12061 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    12121 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    12181 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    12241 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    12301 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    12361 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    12421 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    12481 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    12541 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    12601 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    12661 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    12721 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    12781 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    12841 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    12901 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    12961 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    13021 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    13081 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    13141 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    13201 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    13261 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    13321 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    13381 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    13441 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    13501 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    13561 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    13621 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    13681 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    13741 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    13801 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    13861 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    13921 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    13981 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    14041 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    14101 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    14161 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    14221 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    14281 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    14341 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    14401 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    14461 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    14521 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    14581 tcagacgcga tgcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    14641 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    14701 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    14761 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    14821 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    14881 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    14941 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    15001 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    15061 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    15121 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    15181 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    15241 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    15301 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc
    15361 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    15421 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    15481 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    15541 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    15601 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    15661 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    15721 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    15781 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    15841 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    15901 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    15961 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    16021 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    16081 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    16141 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    16201 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    16261 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    16321 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    16381 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    16441 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    16501 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    16561 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    16621 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    16681 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    16741 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    16801 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    16861 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    16921 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    16981 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    17041 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    17101 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    17161 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    17221 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    17281 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    17341 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    17401 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    17461 cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc
    17521 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaagctgac acgctggcgc
    17581 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    17641 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    17701 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    17761 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    17821 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    17881 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    17941 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    18001 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    18061 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    18121 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    18181 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    18241 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    18301 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    18361 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    18421 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    18481 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    18541 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    18601 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    18661 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    18721 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    18781 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    18841 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    18901 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    18961 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    19021 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    19081 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    19141 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    19201 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    19261 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    19321 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    19381 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    19441 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    19501 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    19561 aacaaagcgg ttcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    19621 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    19681 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    19741 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    19801 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    19861 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    19921 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    19981 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    20041 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    20101 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    20161 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    20221 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    20281 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    20341 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    20401 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    20461 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    20521 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    20581 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    20641 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    20701 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    20761 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    20821 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    20881 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    20941 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    21001 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    21061 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    21121 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    21181 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    21241 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    21301 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    21361 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    21421 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    21481 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    21541 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    21601 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    21661 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    21721 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    21781 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    21841 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    21901 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    21961 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    22021 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    22081 agcgtggagt ctgcgctggc tgactatttt gcggccatcg ccaactgctt taccagcaaa
    22141 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    22201 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    22261 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    22321 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    22381 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    22441 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    22501 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    22561 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    22621 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    22681 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    22741 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    22801 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    22861 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    22921 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    22981 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    23041 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    23101 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    23161 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    23221 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    23281 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    23341 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    23401 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    23461 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    23521 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    23581 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    23641 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    23701 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    23761 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    23821 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    23881 gcagtagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    23941 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    24001 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    24061 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    24121 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    24181 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    24241 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    24301 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    24361 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    24421 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    24481 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    24541 acaatcacaa tctttttcaa tggcgtagtc aacgtgaccc ccttaaattt attaaccaat
    24601 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    24661 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    24721 aagttttgtt gcttaaaaga taggtaactt taccggggga gatcaaaaaa tagagtttat
    24781 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    24841 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    24901 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    24961 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    25021 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    25081 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    25141 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    25201 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    25261 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    25321 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    25381 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    25441 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    25501 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc
    25561 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    25621 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    25681 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    25741 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    25801 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa acccagttcc
    25861 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    25921 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    25981 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    26041 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    26101 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    26161 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    26221 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    26281 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    26341 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    26401 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    26461 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    26521 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    26581 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    26641 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    26701 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    26761 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    26821 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    26881 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    26941 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    27001 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    27061 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    27121 cgcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    27181 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    27241 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    27301 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    27361 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    27421 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    27481 cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    27541 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    27601 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    27661 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    27721 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    27781 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    27841 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    27901 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    27961 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    28021 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    28081 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    28141 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    28201 ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga
    28261 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    28321 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    28381 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    28441 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    28501 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    28561 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    28621 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    28681 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    28741 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    28801 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    28861 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    28921 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    28981 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcatt tgcatttgca
    29041 gcaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca tgagcctgac
    29101 gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata
    29161 tcaagtcatc accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc
    29221 tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc
    29281 tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca
    29341 gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga
    29401 tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca
    29461 ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt
    29521 tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat
    29581 ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg
    29641 taatccatct gcggcaatta atatggttcg aaaacacgcg acctgtcgtg aatttaaagg
    29701 cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag
    29761 cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga
    29821 ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tcgggcattg tcgatgctga
    29881 tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag
    29941 cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg
    30001 cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat
    30061 gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca cccactctga
    30121 agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat
    30181 gctggttggg ccatacagta attatggacc tggctttgat catgtcggcg gcaccggttg
    30241 gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt
    30301 gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgttc
    30361 agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat
    30421 ttcccacaag ccgactggtc atcacagagc ctggcgcagg acgataccac acatatgaaa
    30481 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
    30541 cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
    30601 ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc
    30661 tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc
    30721 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
    30781 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
    30841 ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa aggggtggaa
    30901 tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
    30961 cttgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
    31021 atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac
    31081 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
    31141 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
    31201 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacatgga aggttctatc
    31261 gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg aataacacct
    31321 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacgga ttagtggtta
    31381 cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa aatcgcctga
    31441 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc
    31501 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
    31561 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtt
    31621 gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
    31681 agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat cggtaacagc
    31741 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
    31801 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
    31861 tggatgatgt acaggatcgg cgcaacgaac atgaagaaga actcgatcgg ctcggtgata
    31921 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcacggttt
    31981 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
    32041 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
    32101 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
    32161 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
    32221 aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca
    32281 atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg
    32341 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
    32401 aaacggttaa acatgtacgc tgcaatcgca ccggagataa tccctccgag tacgccagta
    32461 tccgccaagt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
    32521 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
    32581 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
    32641 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
    32701 gcggaaccga cgcccagcag aatacctgcg ataggtagta cggataccgg cagcatcagc
    32761 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
    32821 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta
    32881 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt
    32941 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
    33001 tatgtcacaa tatttcaaac gcggttattc accgcccaat tttcggcggt gaactttact
    33061 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
    33121 gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
    33181 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
    33241 ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat
    33301 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
    33361 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
    33421 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
    33481 agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca
    33541 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa
    33601 taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa
    33661 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
    33721 ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact
    33781 gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
    33841 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg
    33901 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
    33961 cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtgacagaca ttaactgttc
    34021 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
    34081 cagttcggcg accagtcccg gcacatcaac attggtcacc tgcgcagcgc catgatggcg
    34141 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg
    34201 attaagggcc gccagcagcg aataccagtc gcccgatggc acgctatatg ccaacgcctg
    34261 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg
    34321 cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg
    34381 tgaaagccaa gtcacggcgt actgttctgg aggcggcgca aggtaatgta accgacaacg
    34441 actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc
    34501 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc
    34561 agcatcggtt acccagacaa ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc
    34621 ggtgacctca cgtaccgcat caatgcccag cgtatttttc cctttttcgg gagccagggt
    34681 gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    34741 tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    34801 atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata
    34861 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    34921 tccttcaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    34981 gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg
    35041 gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc
    35101 gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc tgggcgaaaa
    35161 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg
    35221 ccctgatacg cctgagtgga aagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    35281 agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact
    35341 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    35401 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    35461 agttgctcga gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc
    35521 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    35581 cacagacttg ttatgactgg caagattggt attaaacgtg tgaccacctt taccatcggc
    35641 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    35701 cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg
    35761 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    35821 ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac
    35881 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag
    35941 ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    36001 tttcaccatt ttcttgtgaa ctcgcttgag taacgcgaca tcggtggtat tggcggtata
    36061 catcaaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    36121 ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg
    36181 caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc
    36241 ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    36301 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac
    36361 ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt
    36421 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    36481 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    36541 aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    36601 tataacgttg ctgacgaaaa ggtgacatcg ccacaggcac gtacgggcat cactggcatt
    36661 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cctccagcga ggcttgcact
    36721 tcgacaagct gataagagga ttgtgccagt aaacggatac agaattgtcg cataatgccg
    36781 ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg ccagaacaaa
    36841 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    36901 tttgtctgct caagatgaga gcgaatcaat acttgctcaa gccgattaag atgtttaata
    36961 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg
    37021 ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg cgttgctggt
    37081 ccgctgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc
    37141 acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag
    37201 tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc gcaggatatg cgccgataac
    37261 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    37321 gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct
    37381 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    37441 ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca acttagatct
    37501 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt
    37561 ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg
    37621 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga
    37681 tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta
    37741 ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    37801 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    37861 ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg
    37921 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    37981 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    38041 gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    38101 gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    38161 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatggc
    38221 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    38281 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    38341 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    38401 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    38461 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    38521 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    38581 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    38641 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    38701 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaagga gggaaaaatg
    38761 attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac
    38821 ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc
    38881 ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt
    38941 gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg
    39001 cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact
    39061 cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa
    39121 aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac
    39181 cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc
    39241 aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa
    39301 agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat
    39361 ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc
    39421 atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt
    39481 aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt
    39541 cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt
    39601 gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa
    39661 aacagatgaa aggttagttt cgataatatc gttccactct tcatctttca ttcgcattaa
    39721 caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc
    39781 gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc
    39841 tttgccgttg gcacctaaat aatcactgat cgcctgagcg ccattttcac tggtcgcagt
    39901 gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg
    39961 gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag
    40021 ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac
    40081 aatgcgtttc gtcaggccag taagcacctt gcccgggccg acttcataga gatgttctac
    40141 gccttgcgct gccatgtact caacagactt cgtccactga accgggttat acaactgacg
    40201 taccagtgcg tcacggatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac
    40261 aacagaaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc
    40321 tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca gcgcgcgttt
    40381 tgcgccagca gctttacagg cagcgccagc acgctcaacc gcttctttat gaccggcaat
    40441 aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc
    40501 ttcacacgct ttcgcaatag gcgcatcatc cagaccgatg attgccgcca tagcgcccgt
    40561 gccttccggt acggcttctt gcatgaactt accgcgcatc tcaaccagac gcaccgcatc
    40621 agcgaaatca atcacgccag cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc
    40681 catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg caacagatgc
    40741 agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg
    40801 ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac
    40861 aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg
    40921 gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcaga
    40981 gccccaagtg aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg
    41041 cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg
    41101 gcgatccagc gtcaccacga cattgtccat cgacataccg agttttttcg ccgttgcact
    41161 gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt
    41221 attcgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac
    41281 ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacgcggt cggcgtttgg
    41341 cagcgtcagc aattcaccat aactaccgtc ggcatgcaga tgggtggaaa tgattcccgg
    41401 ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc
    41461 acgatctgtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt
    41521 caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcaca
    41581 ggctgctgca acgtcaaatg ccgggcaacc tttaatgtcc aacatgcttt gaatctgaca
    41641 agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg
    41701 gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga
    41761 aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca
    41821 ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag
    41881 atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat
    41941 accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt
    42001 tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc
    42061 gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc
    42121 gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc
    42181 cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac
    42241 gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag
    42301 aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct gtaaagccgt
    42361 cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat
    42421 aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcaagaccct
    42481 ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct
    42541 cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtgctg tcacaatcga
    42601 cgttggcccc taaatcgagg accaccgttt tgcccttttg ctgatgtggt aataccgtca
    42661 ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca
    42721 gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca
    42781 gggccatacg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac
    42841 tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt
    42901 tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat
    42961 tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca
    43021 tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc
    43081 cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc
    43141 gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc
    43201 accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg
    43261 catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca
    43321 attacggttt atccccgatc caacggggag caccaattac ttacgcttta agctggctaa
    43381 tacggcaaat gggtttggct tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc
    43441 ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat
    43501 ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc
    43561 atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt
    43621 tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac
    43681 cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca
    43741 ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt
    43801 atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg
    43861 taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa
    43921 aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta
    43981 ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg
    44041 gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc
    44101 acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca
    44161 cctgaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg
    44221 gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca
    44281 gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc
    44341 cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg
    44401 tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt
    44461 cacctaagcg aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg
    44521 ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac
    44581 cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg
    44641 aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc
    44701 ggtgcagacg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct
    44761 tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc
    44821 catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc
    44881 ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc
    44941 acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg
    45001 gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg
    45061 ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg
    45121 ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg
    45181 ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc
    45241 atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg
    45301 gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc
    45361 gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag
    45421 caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag
    45481 atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc
    45541 atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcacc
    45601 tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa
    45661 gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt
    45721 ctctgttttc atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct
    45781 atgcttatat ttagttttgc accttattac ttcactacgt gatcacttta ttgatggtta
    45841 ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa
    45901 agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct
    45961 tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca
    46021 atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc
    46081 ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt
    46141 ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt
    46201 gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt
    46261 gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa
    46321 tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg
    46381 acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa
    46441 tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg
    46501 gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg
    46561 gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata
    46621 aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct
    46681 atctggttct gatgccaaat aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg
    46741 atgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa ggcatggggc
    46801 ttatcgtgcg caccgctggc gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct
    46861 tccgcctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg gctccgttcc
    46921 tgattcatca ggagagcaac gtaatcgttc gcgccttccg cgattattta cgtcaggata
    46981 tcggcgaaat ccttatcgat aacccgaaag tcctcgaact ggcacgtcag catatcgctg
    47041 cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag atcccgctgt
    47101 tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc
    47161 catccggtgg ttccattgtt atcgacagca ccgaagcgtt aacagccatc gacatcaact
    47221 ccgcacgcgc gacccgcggc ggcgatatcg aagaaactgc gtttaacacc aacctcgaag
    47281 ccgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg
    47341 acttcatcga catgacgcca gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag
    47401 cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg
    47461 aaatgtcccg ccagcgcctg agcccatcac tgggtgaatc cagccatcac gtctgcccgc
    47521 gctgctccgg taccggtacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtt
    47581 tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc
    47641 caatcgcttc ttatctgctg aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc
    47701 aggacggtgt tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg cactaccacg
    47761 tgttgcgcgt gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc
    47821 atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac
    47881 aacctgcact ggcaaccttt gccatgccgg atgtaccgcc agcgccaacc ccagctgaac
    47941 ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca gcagctcctg
    48001 cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg
    48061 aagaagccaa accgaccgag caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac
    48121 aggatcgtcg caaacctcgt cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc
    48181 gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg
    48241 cacagcagca aactgccgag acgcgtgaga gccgtcagca ggctgaggta acggaaaaag
    48301 cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg
    48361 ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg
    48421 aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca
    48481 atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg
    48541 aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc acagaactgg
    48601 tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg
    48661 ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc
    48721 gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa
    48781 tgccgttgac cgtagcgtgc acgtctccgg aattggcctc tggcaaagtc tggatccgct
    48841 atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag gaagaagtac
    48901 aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg
    48961 cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc
    49021 aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta actgaacagc
    49081 cgcaggtgat taccgagtct gatgttgccg tagcccagga agtcgcagaa cacgcagaac
    49141 cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa actgctgagg
    49201 ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg
    49261 cagcagaagt tgaaacggtg gccgcggttg aaccagagat caccgttgag cataaccacg
    49321 ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca ccgcgtcaca
    49381 gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg
    49441 caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc
    49501 aaagtaatca agccctggta actgccaggg cttttttatt tcatgtttga atcaacatat
    49561 taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa aaaaatgccc
    49621 gccccggcga tcccagagcg ggcagacaaa acatacccag tttcatgata tgttttaaag
    49681 cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac
    49741 gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca
    49801 tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc
    49861 agcgactcca gtttgttcag ctgcgtgcct aattccgcgc gaacggtaag gacattattc
    49921 agcgagtttt tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc
    49981 gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa
    50041 agattggttt cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg
    50101 aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgctta
    50161 atactttctg ttccgccaac ataatcaccg ttagcctcac taaacggcgc agtctctgtt
    50221 ttataaccgg caaaaatgta gcgcccatta ccgtcagtgg tgttcgccag attcagcaac
    50281 tggtcacgaa gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg
    50341 ccattgctgg cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta
    50401 agtacactct cttccagtga cactttttga gtggcgaaag tacgtgccag cgtgtactgg
    50461 ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg atgcagcaat gggatcgtct
    50521 gaagggttaa cgactcgctt acccgtcgac atctgctcgc cgtacttcat ccattctgcc
    50581 tgagaattgg tgatgccgcg catgttttgc tggtacatca tctgggtact gaaacgcatt
    50641 ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa
    50701 cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga
    50761 gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg
    50821 tggtgctact ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat
    50881 aagcgtcgtt aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca
    50941 gggcctgacc gttgcggttg tcgctatcac ccgcatcttc ttcgctcgcc atcgcgattt
    51001 tcgcttcgtc ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg
    51061 tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca
    51121 ctttaccgtt ggcatctggt gtcgccgtaa aagtggtatt gctggcaagg cgggtgacct
    51181 gccactgatt attatcgaac gagattttgt aatctgtcgc cagtaccacg gaggcatcag
    51241 ttaccgtggc accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct
    51301 taccgatagt aaagaaatct tcaccagcat cgccgttagc atcaaatccg gctttgtgtt
    51361 ggctgttgaa agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt
    51421 ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct
    51481 ccgggatctc aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt
    51541 cggcgctgga aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac
    51601 cattggcatc gtgatgttat aagtaccgcc atcctgaacg ctgacttcta cgccaacaat
    51661 ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc
    51721 tgcccccacg cctgtcaggc gcgagatttg atcgttcagg ctggcaattt gtttagcgta
    51781 attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg
    51841 cagatattga tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc
    51901 ctggcgcgcc gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc
    51961 ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat
    52021 ctgctcatag cgggcggtca ggccgctact ttgcgtctgc gcagcacgta actggttggt
    52081 aataaacgta tcatactcac gctgcacacc agaaacgtag acgccattgc caacccagcc
    52141 gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc
    52201 aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcgttcag
    52261 tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc cttcaattac
    52321 agagtattat cggcagcgac ccgccggact tgagttattc agaacagatt atcaatgttc
    52381 atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctggat catgttggtg
    52441 agtttacggg catagtgagg atcggtagca tagcccgcgt cctgtaggac ctgcgccccc
    52501 tgttccgcac tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca
    52561 acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaacttcgc ttttactttc
    52621 ttcgcttcgc cgttttcata ttcagtcgtg gtgatttcag tgacttgccc tttccagttg
    52681 ccagaggctt tgacaccaaa caggttatag cttggctcgc cgttttcgcg gcggatttgc
    52741 cgttgccccc aaccagattc aagcgcagcc tgagcgagga tcaaatgatg tggcacaccg
    52801 ctttgctggc ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tctactgtca
    52861 cccggcagcg aatcatcgta gttacgtggt acggcctttt gcaccagctg cgaaagcgtc
    52921 tggttttgat aacgcaccac ggtttcgagc gggaatttca tcggtgctgc tggcgtggac
    52981 tcctctggca atggttgttc tggcgtcatc tgtttaacca tcatctctgc aagccccaga
    53041 cctttgcccg ccgtcatctg ttgggcaatt tgctggtcat acatactggt atacagacga
    53101 gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc
    53161 atctgcacga acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct
    53221 tcgctcgctt tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt
    53281 agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt
    53341 gcattgattg caaaatagac atcagatcca tcggcgtagc gcccagcgca ttgagcgcac
    53401 gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc
    53461 gtaaatcgat ctgcgtttgt ggagtaacaa cagtctgtcc accaccaaac ggtgtatctg
    53521 gctggctgac attggcctga cgattaactg ttactgagag atttccctgc gctaccgcgc
    53581 agctgtcgag ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta
    53641 ctttagcgtc ctgcggggtg acattaacct gcatattctg gatatcggca aggaaacgga
    53701 cctgggaact gttgccactt ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg
    53761 cgctgccata tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat
    53821 cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggcaattcac
    53881 gttcaataac cgcaccattg gtgatccgtc caccgttcag ttggttaacc tgaacactgc
    53941 taccgcctgc ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca
    54001 cctgactgtc aacgccctta agcggtgtca tcaacaacgt acctccacgc aagcttttgg
    54061 cattacccat ggaagaaacc accacatcga tggtttgccc ctgacgtcca aacggaggaa
    54121 gtgacgctgt caccattacc gcagcgacgt tttttagctg catattggtg cccgtcggaa
    54181 cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg
    54241 ttgtctggtc accagtgcca tccagcccca ctaccaagcc atagccaatc agtgagtttt
    54301 gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa
    54361 ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga
    54421 caggttaagg aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt
    54481 gcctacgtat tcaatacgcg catccgccac ctgagtagac ggtacggtat tgctgccgct
    54541 gatagtgcgt ggattaacca cgcccgagaa gcgaataaat tcggtaccct gattaatggc
    54601 aatctgtttt tcacccacca catgcaggtt gctgttgacc agtacctggt caaccgtcac
    54661 cgtcaacgtg ccgctaaagg tattgctggc attggccccg ccctttccgt tgaacgtgtt
    54721 accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg
    54781 cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt
    54841 gctggcgctg acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg
    54901 acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga
    54961 accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag
    55021 cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc
    55081 ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt aaaccggtga
    55141 acctgccacc ggttatgcct tagagttgcg tcagtttttg cagcatctga tcggtggtgg
    55201 acaccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt
    55261 ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc gcgccgttca
    55321 ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag aggttttcgc
    55381 caatgctctc cagtccggta tcattcataa aggtggtgag attgagctgc ccaacctgaa
    55441 ccggagctgc ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac
    55501 ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gccgtcacca
    55561 gctgcccgtt ctgatccacc tggaaagagc cgtcacggat ataggctgat gagccatctg
    55621 gcaacattac ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttattggtct
    55681 gcgacagatt gccctggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga
    55741 tttgtaatcc ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg
    55801 tttgataaag cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt
    55861 ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg
    55921 cgatccataa tgaactgatc atataatgtc ctgtagtgga ttgctgacaa gcagagcaga
    55981 agcacgcgac agtgtgaatt tcccctcacc ctaaccctct ccccaaaggg gcgagggaat
    56041 agtccgcgcg cttttattgc aatgtcatca atcgttcaat taactcatcg acagcagttg
    56101 gttggcacgg cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg
    56161 ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag
    56221 aacccccgac atcacacgca aggttggatc tgcctgcagt accggccctc gcgtggcctg
    56281 ggtttctgcg cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc
    56341 tttcaccagt ttcagacgcc ctactggcgc aactgtattt gccggatcgc ccggattgag
    56401 cgccgagatt gtgccatcgg cagcaatcgt gatttccgcc ccttccggca cagcaattgg
    56461 ccccgcctcg cctatcaccg gatgcccctg aattgtcagt tgcccggtgg gatcaacctg
    56521 aatgctgcca ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccagccatcc
    56581 tgctgcaacg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct
    56641 gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc
    56701 actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta
    56761 ctggcggtta ccgcctgttg attcagcgtc tggctggctg ctcccatcgc agtataaatt
    56821 gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaacccgcg tgttgaggat
    56881 ctggtcctgg gttttgatgg tctgggcgtt agacttatag ttacgctggg caacgatcat
    56941 attgaccagt tctttactga gatcgacgtt ggacgcttcc agcgcaccgt tggtcagggt
    57001 gccaaagttt cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga
    57061 ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat
    57121 ctgccccagc agttgggttt gttcgttgga attgttgccg acaaccgtac cgtcatcatt
    57181 gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatgtt
    57241 gttagcgccg gtattttgct gcatggagtt gaggaagctc agactaaacg tggcgggttc
    57301 tgcaccgtta attgcgccgg ttgcgatatt attcgccatc gcaccatcca ctaatgtgcc
    57361 attagcatta aattccagtg ttgtcgctgt cttcgcaatg ctgtttggat cactgctatc
    57421 ctgggtgtag acctgccagt tattatcccc ggtcttcaca aaatagacgc tcatgtcatg
    57481 agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt
    57541 gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt tgatctgcat
    57601 cgacgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg
    57661 attcgcccct tgctgaatag tcggcggcgt accggttacc gggtaacccg tcagctgtaa
    57721 accttgcgta ttcaacaggt tacggttttc atccagctta aattgtccgt tacggctgta
    57781 gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc
    57841 cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccggcaac
    57901 ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga
    57961 tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc
    58021 ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa
    58081 ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag
    58141 cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca gcggctgggc
    58201 aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac cgttcggcgc
    58261 agtgctgcca tcagtcaacg aaccgtccta ggtgaaactg tgaactccgg cggtcagttc
    58321 accaatatca atggtgcgca caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt
    58381 gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag ccccttcttc
    58441 actgccggtt cccgcaagaa cagttgtgcc ggggatcatc acgccgtgac cgatcaggtt
    58501 actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga gcgtggtatt
    58561 gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt
    58621 ttccattgga ttggtcgggt cctggttttt cagctgcgcc accagcaaag tcagaaaact
    58681 gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc
    58741 ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg
    58801 agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac attggcctga
    58861 tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac gttcggcatt
    58921 tttacgtagc cctttgcatc tgccagcgga ttacctggtt cataaaccag tttgtccggg
    58981 gcctgacttt cttaacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    59041 taacctggaa taccacctgt tttgcccgat atggctgtcc atcaggaccg gtcacgctat
    59101 cagcatttgc cagattactg gccgccacgt tcaggcgctg ggactgggca gttaacgccg
    59161 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    59221 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    59281 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    59341 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccgggatgt
    59401 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    59461 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    59521 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    59581 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    59641 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    59701 ctatttatgc cgttgatggt cattgcggac aggtacaatt cacgttgtag aaatggctgg
    59761 gggcgaaaat gctgacaata aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt
    59821 taagtacggc gagcaatctc acatcgcaat tgcacaactt ttttagcgcc caactcgcgg
    59881 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccatgcg
    59941 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    60001 cacgctgcgg taacgacaaa cgatatttac aggttaatgt gcaggccaca ggaaattatg
    60061 tggttgccgc gatgcctatt gtgcggggag gaaagctgga agctggcaat gtcaaactga
    60121 aacgtggacg cctggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    60181 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    60241 aggcatggcg ggtaaaagcg ggacaacgcg tcaatgtgat cgccagcggt gatgggttta
    60301 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc
    60361 gcatggtatc gggacaggta gtcagcggcg ttgttgatgc agatgggaat attcttataa
    60421 acctgtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    60481 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcctgtaa
    60541 gcaccgttca accgcgcgaa accaccgacg cgccggtaac gaacagccgg gcggcaaaaa
    60601 caaccgcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    60661 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    60721 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag
    60781 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    60841 gtgcttaacg atctcaaaac ggtaatggat caagagcagc aacatctctc tatggggcag
    60901 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    60961 gattacctcg aacagttacg caagaaagaa cccaatacag caaatagcgt tgatattagt
    61021 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    61081 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    61141 catcaggaac cgacgctata tggggcgaac ggtcagacct caacacccca tcgcggcagt
    61201 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    61261 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    61321 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    61381 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    61441 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    61501 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    61561 tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    61621 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    61681 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    61741 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    61801 cggacctgaa agaatgccca acgcaaccgc actcggcagt gccaacagga aacaaagacg
    61861 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    61921 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    61981 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    62041 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    62101 tttcaccacg cgcattgctc cagcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    62161 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    62221 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    62281 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    62341 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    62401 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    62461 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    62521 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    62581 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    62641 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    62701 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    62761 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccgga cccacgggca
    62821 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctagatta tggtgaattg
    62881 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    62941 tacgttgcgc cagcactgcc tattcgccgg cggtttgcgg aactgtctga ttttgcacgc
    63001 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    63061 caggaatcgg tttattcact acaccctgcc cgcgctcctc acgccattcg cgcatatccg
    63121 taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    63181 ggcgatgcat acaagtggtg atctgcaaag gaccggccga aaaatggtgc tcggcaaaca
    63241 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    63301 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    63361 cgtgtggccc gacgctattg ctacgatgtt tatccattct tagcgaggct gcggtggcga
    63421 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    63481 tcagtttttt ccgcgccgcc agttccacca gcctttcagc atcgcgcaga ttttctgcca
    63541 gcggtttatc gacacagaca tgtacccccg cattgagtaa cgtactgacc acgtcaaagt
    63601 ggctggcggt gctggagtgc acaaaaaccg catcacaact ggcggcaagg ctggataacg
    63661 aatcggcata aggaatgcgc cagctgtcac aaattggcag ggctttggcg cgcgtaggcg
    63721 accaggctcc ttgtaacgtc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    63781 gcgcaatgcc gcctaatccc actacgccga tacgtaattt tttcacttta atctcccaga
    63841 tgggccagca acgaatcaag acgttgtttc agttctgcca cttcgatttc cagggcttcg
    63901 acgcgggtct ataaatcacc gtcaaccatg ttcgacatag ccgttaccgc cggctgatct
    63961 tcaacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    64021 agacgcacca caaaaggacc atcttcgcga ttcgccagtt gttccagcgt cgactccact
    64081 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    64141 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ctgcggcgct cagtttcaga
    64201 tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg tgacccgatt accaaaaccg
    64261 ctcactgtgc gtagataatg acgtttgacc agattatcca gctgttcctg cacttcggat
    64321 tcactcagat tcatgaccgg tttacggttc gttttctgat tacaggccgt gactacacca
    64381 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatt
    64441 acgcgagctt ccaaggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    64501 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    64561 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    64621 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    64681 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    64741 cgaataaccg agatagcagg catgaaaaga gccgcgaaca acattggaaa aattggcaac
    64801 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    64861 atgaaattcg ttaatcatcc ctagcctggc ctgccagcct gatggataac agtggctttc
    64921 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgccag
    64981 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    65041 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    65101 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    65161 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    65221 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    65281 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    65341 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    65401 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    65461 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    65521 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    65581 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    65641 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    65701 acgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccagtgcgg tcattggcgc
    65761 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    65821 tttgtttgtg ctatgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    65881 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    65941 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    66001 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    66061 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    66121 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    66181 catggtcagc ggcctacaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    66241 cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg
    66301 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    66361 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    66421 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    66481 cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    66541 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    66601 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    66661 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    66721 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    66781 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    66841 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    66901 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    66961 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    67021 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    67081 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    67141 caactggcca agccgcgttg ctaattaccg cgataatatg gcgaaacaga cacaaatcaa
    67201 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    67261 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    67321 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    67381 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    67441 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    67501 tacccggcgt ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg
    67561 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    67621 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    67681 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    67741 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    67801 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    67861 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    67921 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    67981 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
    68041 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    68101 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    68161 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    68221 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    68281 atcctaatga gctggagcgc ggatttaatg aagccgtgtt caccgctgca aaactttacc
    68341 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    68401 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    68461 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    68521 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    68581 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    68641 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    68701 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    68761 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    68821 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    68881 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
    68941 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    69001 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    69061 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
    69121 cagtatgttc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    69181 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    69241 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    69301 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    69361 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccggatcg
    69421 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    69481 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    69541 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    69601 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    69661 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    69721 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    69781 gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac
    69841 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    69901 tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    69961 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    70021 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    70081 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    70141 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    70201 ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg
    70261 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    70321 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    70381 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    70441 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    70501 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    70561 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    70621 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    70681 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    70741 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    70801 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    70861 agcggatgaa cgtgttccgt ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt
    70921 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    70981 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    71041 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    71101 cgattttgcg caagaccaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    71161 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    71221 cggctgttgc actatctcat caataatatt cgcgagcatt tgatgcttta tctttttctc
    71281 tggggattgt tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg
    71341 acataaactg acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgatatta
    71401 ttcctgcttc tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag
    71461 attctttcat tattaattca aaattattga aataaaaata aatatcattc aatttgttat
    71521 ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg
    71581 atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc
    71641 attggctaag cgcaattatt gtctacggca tgtttgcttt aggtctgtgg atggtcacgc
    71701 tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt
    71761 tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc
    71821 cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa agctggacat ctcgcattgt
    71881 acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac
    71941 cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg
    72001 cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtctgtta
    72061 tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca
    72121 tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt
    72181 taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg
    72241 acaaagaagg tcagcacgcc tttgttaact tccgcataca gcatcttggc tatagctggt
    72301 tatatggcac ctttaaagat ttcgacggta cttttacctt tgatgaaaaa aatccggctg
    72361 ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaaccac gccgaacgcg
    72421 ataaacatct tcgcagtgcg gattttctca ataccacaaa atatccacag gcaacattca
    72481 cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga
    72541 atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat
    72601 ggggcggtaa acgtgcaggc tttgaggccg aaggcaaaat taagctcaaa gacttcaata
    72661 tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg
    72721 tacagcagaa gtaagagtga aatgttgccg gatggcacgc atccggcaat gatatttatt
    72781 ctgttggatc aggaataccc agtgttgtat tcagacgtcc acgagactta ttaaagatct
    72841 tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt
    72901 cttcgcagca aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa
    72961 taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc
    73021 actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa
    73081 caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt
    73141 caataattcc gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaggcac
    73201 tggccttttc acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg
    73261 cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga
    73321 gcgcgttttc aaagtgcccc acttcatact catagtggtt acgcatgtcg ataaatagtg
    73381 catcgggatc gtcaagcatg gcgttcactt ccgccgcttg cagatactca ccaacattgc
    73441 tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt accttcatgc
    73501 gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta
    73561 aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc
    73621 tgatttgcgc gttaatgccc tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg
    73681 taaacagctg atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt
    73741 aaaacgaaat ggtggttcgc ggttcgctct cagccaacat tttggctttt aacgcgtcgt
    73801 tggaaatgcg gttgtgtaac actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa
    73861 aatccgcggc atgatatagc aattatcgat aattaacatc cacacatttt acgctacatt
    73921 tgcgcattaa aaattatttg ttatttacaa gcgcggcaat ttcgcccagt cttcagccac
    73981 aaattttggt tgcgggcgaa aaaatgcgac aatacataca attgcccgta taggttgaaa
    74041 aacaggattg atatgacgaa tctacccaag ttctccaccg cactgcttca tccgcgttat
    74101 tggttaacct ggttgggtat tggcgtactt tggttggtcg tgcaattgcc ctacccgatt
    74161 atctaccgcc tcggttgtgg attaggaaaa ctggcgttac gttttatgaa acgacgcgca
    74221 aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt
    74281 aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc tgatggaaac cggtatggcg
    74341 tggttctggc cggaccgccg aatcgcccgc tggacggaag tgatcggcat ggaacacatt
    74401 cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg gcatccattt tctgacactg
    74461 gagctgggcg cgcggcagtt tggtatgcag gaaccgggga ttggcgttta ccgcccgaac
    74521 gataatccac tgattgactg gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg
    74581 ctcgaccgca aagatttaaa aggcatgatt aaagccctga aaaaaggcga agtggtctgg
    74641 tacgcaccag atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt
    74701 gagcaggctg cgaccacgac cggaacctgg atgctggcac ggatgtccgg cgcatgtctg
    74761 gtgcccttcg ttccacgccg taagccagat ggcaaagggt atcaattgat tatgctgcca
    74821 ccagagtgtt ctccgccact ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg
    74881 gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt ggttacaccg tcgctttaaa
    74941 acacgcccgg aaggcgttcc ttcacgctat taaatctccc atgccggatg cttcagaatg
    75001 gcatccggca ttaccacagc aaatccccct gatttagcga taaaagctct ctggattgcg
    75061 ccccctggaa gtcgggcgca taattagtgt gtttatcttt tcttcttatg ttcaccgcgc
    75121 ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa atgacacccc tataaactgg
    75181 aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta ccggggccgc cttcagtctg
    75241 gtaatgccct tcttacccct ctacgttgag cagcttggtg ttaccggcca ctccgccctg
    75301 aatatgtggt ccggtattgt cttcagtata atgggttcct aaacacatca ttttaggtat
    75361 tgaatatgag acgggcaatc agctacatca gattttcaag tgaacgacaa ctgaaagggg
    75421 actctgtacg ccgacaatct aagctggtta ctgactggct tgataagaac cctgagtttt
    75481 acttggattc atccttgagc tttaaagatt tggggaaaag tgcttttagt ggtaagcacc
    75541 tgaaaggtgg tctaggtgac tttttaaccg ctatcgagaa agggcttgtt aaagctggtg
    75601 atacgctact aatcgagagt ttagaccgtc tgtcacgtca ggatattgat attgcttctg
    75661 agctattacg ccgtatctta agggctggtg tagatgtagt cacactgagc gatggagaac
    75721 attacacacg agaaagttta aaagacccgt tagccctgat taaaagcatt ctgataatgc
    75781 agcgggcgca tgaggaaagt ttgagaaagt ctgagcgtgt ccaagctgca tggaaccgta
    75841 aaaaagaact catctccgaa ggtatcaagg tat
//
LOCUS       DADLDT020000003        71502 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000003 DADLDT020000000
VERSION     DADLDT020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 71502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71502
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.003"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(750..2345)
                     /locus_tag="JQH22_000162"
     CDS             complement(750..2345)
                     /locus_tag="JQH22_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFB2204622.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="JQH22_000163"
     CDS             complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="JQH22_000163"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFB2204623.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4893..5297)
                     /locus_tag="JQH22_000164"
     CDS             complement(4893..5297)
                     /locus_tag="JQH22_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HFB2204624.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="JQH22_000165"
                     /pseudo
     CDS             complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="JQH22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="JQH22_000166"
     CDS             complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="JQH22_000166"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFB2204625.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="JQH22_000167"
                     /pseudo
     CDS             complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="JQH22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9339..10109
                     /gene="glcC"
                     /locus_tag="JQH22_000168"
     CDS             9339..10109
                     /gene="glcC"
                     /locus_tag="JQH22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFB2204626.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10233..11374)
                     /locus_tag="JQH22_000169"
                     /pseudo
     CDS             complement(10233..11374)
                     /locus_tag="JQH22_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11736..13466
                     /locus_tag="JQH22_000170"
     CDS             11736..13466
                     /locus_tag="JQH22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFB2204627.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13463..14377
                     /locus_tag="JQH22_000171"
     CDS             13463..14377
                     /locus_tag="JQH22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFB2204628.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14410..14658
                     /locus_tag="JQH22_000172"
     CDS             14410..14658
                     /locus_tag="JQH22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFB2204629.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14658..15829
                     /locus_tag="JQH22_000173"
                     /pseudo
     CDS             14658..15829
                     /locus_tag="JQH22_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15865..16944)
                     /gene="lptG"
                     /locus_tag="JQH22_000174"
     CDS             complement(15865..16944)
                     /gene="lptG"
                     /locus_tag="JQH22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFB2204630.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16941..18011)
                     /gene="lptF"
                     /locus_tag="JQH22_000175"
     CDS             complement(16941..18011)
                     /gene="lptF"
                     /locus_tag="JQH22_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFB2204631.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18042..18602)
                     /locus_tag="JQH22_000176"
     CDS             complement(18042..18602)
                     /locus_tag="JQH22_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFB2204632.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18614..19438)
                     /locus_tag="JQH22_000177"
     CDS             complement(18614..19438)
                     /locus_tag="JQH22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204633.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19438..20787)
                     /locus_tag="JQH22_000178"
     CDS             complement(19438..20787)
                     /locus_tag="JQH22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFB2204634.1"
                     /translation="MAGFNIKHWFADGAFRTMIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20833..21591)
                     /locus_tag="JQH22_000179"
     CDS             complement(20833..21591)
                     /locus_tag="JQH22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204635.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21623..22336)
                     /locus_tag="JQH22_000180"
     CDS             complement(21623..22336)
                     /locus_tag="JQH22_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFB2204636.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22510..23202
                     /locus_tag="JQH22_000181"
     CDS             22510..23202
                     /locus_tag="JQH22_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFB2204637.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23251..24750)
                     /gene="pitB"
                     /locus_tag="JQH22_000182"
     CDS             complement(23251..24750)
                     /gene="pitB"
                     /locus_tag="JQH22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFB2204638.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25042..26901)
                     /gene="gss"
                     /locus_tag="JQH22_000183"
     CDS             complement(25042..26901)
                     /gene="gss"
                     /locus_tag="JQH22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFB2204639.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27106..27972
                     /gene="yghU"
                     /locus_tag="JQH22_000184"
     CDS             27106..27972
                     /gene="yghU"
                     /locus_tag="JQH22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFB2204640.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28095..28343)
                     /gene="hybG"
                     /locus_tag="JQH22_000185"
     CDS             complement(28095..28343)
                     /gene="hybG"
                     /locus_tag="JQH22_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFB2204641.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28477..28818)
                     /gene="hypA"
                     /locus_tag="JQH22_000186"
     CDS             complement(28477..28818)
                     /gene="hypA"
                     /locus_tag="JQH22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFB2204642.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28811..29299)
                     /gene="hybE"
                     /locus_tag="JQH22_000187"
     CDS             complement(28811..29299)
                     /gene="hybE"
                     /locus_tag="JQH22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFB2204643.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29292..29786)
                     /locus_tag="JQH22_000188"
     CDS             complement(29292..29786)
                     /locus_tag="JQH22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFB2204644.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29786..31489)
                     /gene="hybC"
                     /locus_tag="JQH22_000189"
     CDS             complement(29786..31489)
                     /gene="hybC"
                     /locus_tag="JQH22_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFB2204645.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31486..32664)
                     /gene="hybB"
                     /locus_tag="JQH22_000190"
     CDS             complement(31486..32664)
                     /gene="hybB"
                     /locus_tag="JQH22_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFB2204646.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32654..33640)
                     /gene="hybA"
                     /locus_tag="JQH22_000191"
     CDS             complement(32654..33640)
                     /gene="hybA"
                     /locus_tag="JQH22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFB2204647.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33643..34761)
                     /gene="hybO"
                     /locus_tag="JQH22_000192"
     CDS             complement(33643..34761)
                     /gene="hybO"
                     /locus_tag="JQH22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFB2204648.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34950..35237)
                     /locus_tag="JQH22_000193"
     CDS             complement(34950..35237)
                     /locus_tag="JQH22_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HFB2204649.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35356..36243)
                     /locus_tag="JQH22_000194"
     CDS             complement(35356..36243)
                     /locus_tag="JQH22_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFB2204650.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36400..37440
                     /gene="gpr"
                     /locus_tag="JQH22_000195"
     CDS             36400..37440
                     /gene="gpr"
                     /locus_tag="JQH22_000195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFB2204651.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37480..37974)
                     /locus_tag="JQH22_000196"
     CDS             complement(37480..37974)
                     /locus_tag="JQH22_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFB2204652.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38165..39049
                     /gene="yghA"
                     /locus_tag="JQH22_000197"
     CDS             38165..39049
                     /gene="yghA"
                     /locus_tag="JQH22_000197"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFB2204653.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39321..39746)
                     /gene="exbD"
                     /locus_tag="JQH22_000198"
     CDS             complement(39321..39746)
                     /gene="exbD"
                     /locus_tag="JQH22_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFB2204654.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39753..40487)
                     /gene="exbB"
                     /locus_tag="JQH22_000199"
     CDS             complement(39753..40487)
                     /gene="exbB"
                     /locus_tag="JQH22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFB2204655.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40739..41926
                     /gene="metC"
                     /locus_tag="JQH22_000200"
     CDS             40739..41926
                     /gene="metC"
                     /locus_tag="JQH22_000200"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFB2204656.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42066..42725
                     /locus_tag="JQH22_000201"
     CDS             42066..42725
                     /locus_tag="JQH22_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2204657.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42765..43664)
                     /gene="yqhC"
                     /locus_tag="JQH22_000202"
     CDS             complement(42765..43664)
                     /gene="yqhC"
                     /locus_tag="JQH22_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFB2204658.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43858..45021
                     /gene="yqhD"
                     /locus_tag="JQH22_000203"
     CDS             43858..45021
                     /gene="yqhD"
                     /locus_tag="JQH22_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFB2204659.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45126..45953
                     /gene="dkgA"
                     /locus_tag="JQH22_000204"
     CDS             45126..45953
                     /gene="dkgA"
                     /locus_tag="JQH22_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFB2204660.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46153..47079
                     /locus_tag="JQH22_000205"
     CDS             46153..47079
                     /locus_tag="JQH22_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFB2204661.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47130..47387
                     /gene="yqhH"
                     /locus_tag="JQH22_000206"
     CDS             47130..47387
                     /gene="yqhH"
                     /locus_tag="JQH22_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFB2204662.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47430..49649)
                     /locus_tag="JQH22_000207"
     CDS             complement(47430..49649)
                     /locus_tag="JQH22_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFB2204663.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49760..51172)
                     /gene="ftsP"
                     /locus_tag="JQH22_000208"
     CDS             complement(49760..51172)
                     /gene="ftsP"
                     /locus_tag="JQH22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFB2204664.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51247..51984)
                     /gene="plsC"
                     /locus_tag="JQH22_000209"
                     /gene_synonym="parF"
     CDS             complement(51247..51984)
                     /gene="plsC"
                     /locus_tag="JQH22_000209"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFB2204665.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52218..54476)
                     /gene="parC"
                     /locus_tag="JQH22_000210"
     CDS             complement(52218..54476)
                     /gene="parC"
                     /locus_tag="JQH22_000210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFB2204666.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55022..55504)
                     /locus_tag="JQH22_000211"
     CDS             complement(55022..55504)
                     /locus_tag="JQH22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFB2204667.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55557..55949)
                     /gene="ygiW"
                     /locus_tag="JQH22_000212"
     CDS             complement(55557..55949)
                     /gene="ygiW"
                     /locus_tag="JQH22_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFB2204668.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56101..56760
                     /gene="qseB"
                     /locus_tag="JQH22_000213"
     CDS             56101..56760
                     /gene="qseB"
                     /locus_tag="JQH22_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HFB2204669.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56757..58031
                     /gene="qseC"
                     /locus_tag="JQH22_000214"
     CDS             56757..58031
                     /gene="qseC"
                     /locus_tag="JQH22_000214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HFB2204670.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58077..58409)
                     /locus_tag="JQH22_000215"
     CDS             complement(58077..58409)
                     /locus_tag="JQH22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFB2204671.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58728..59309
                     /gene="mdaB"
                     /locus_tag="JQH22_000216"
     CDS             58728..59309
                     /gene="mdaB"
                     /locus_tag="JQH22_000216"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFB2204672.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59340..59654
                     /locus_tag="JQH22_000217"
     CDS             59340..59654
                     /locus_tag="JQH22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFB2204673.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59702..61594)
                     /gene="parE"
                     /locus_tag="JQH22_000218"
     CDS             complement(59702..61594)
                     /gene="parE"
                     /locus_tag="JQH22_000218"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFB2204674.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61623..62204)
                     /gene="yqiA"
                     /locus_tag="JQH22_000219"
     CDS             complement(61623..62204)
                     /gene="yqiA"
                     /locus_tag="JQH22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFB2204675.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62204..63031)
                     /gene="cpdA"
                     /locus_tag="JQH22_000220"
     CDS             complement(62204..63031)
                     /gene="cpdA"
                     /locus_tag="JQH22_000220"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFB2204676.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63056..63478)
                     /locus_tag="JQH22_000221"
     CDS             complement(63056..63478)
                     /locus_tag="JQH22_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFB2204677.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63479..64108)
                     /gene="nudF"
                     /locus_tag="JQH22_000222"
     CDS             complement(63479..64108)
                     /gene="nudF"
                     /locus_tag="JQH22_000222"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFB2204678.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64313..65794
                     /gene="tolC"
                     /locus_tag="JQH22_000223"
     CDS             64313..65794
                     /gene="tolC"
                     /locus_tag="JQH22_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFB2204679.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65942..66613
                     /locus_tag="JQH22_000224"
     CDS             65942..66613
                     /locus_tag="JQH22_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFB2204680.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66619..67779
                     /locus_tag="JQH22_000225"
     CDS             66619..67779
                     /locus_tag="JQH22_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFB2204681.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67817..68605)
                     /gene="ygiD"
                     /locus_tag="JQH22_000226"
     CDS             complement(67817..68605)
                     /gene="ygiD"
                     /locus_tag="JQH22_000226"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFB2204682.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68748..69521
                     /gene="zupT"
                     /locus_tag="JQH22_000227"
     CDS             68748..69521
                     /gene="zupT"
                     /locus_tag="JQH22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFB2204683.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69579..69749)
                     /locus_tag="JQH22_000228"
     CDS             complement(69579..69749)
                     /locus_tag="JQH22_000228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204684.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70010..70663)
                     /gene="ribB"
                     /locus_tag="JQH22_000229"
     CDS             complement(70010..70663)
                     /gene="ribB"
                     /locus_tag="JQH22_000229"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFB2204685.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70767..70915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71037..71327
                     /gene="ubiK"
                     /locus_tag="JQH22_000230"
     CDS             71037..71327
                     /gene="ubiK"
                     /locus_tag="JQH22_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFB2204686.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17836 a        19059 c        17663 g        16944 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaat atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gccgataacg ctggcaaaaa tcagactgtg cttcacggtg
      841 tagcggaaca gttcagattc tcggcccacc atgcccgtcg cggcgcaggc cacggcgata
      901 gattgcgggg agatcatctt gccagttacg ccgccgctgg tatttgctgc caccagcaga
      961 gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca gtgaaccaaa cagggcgttt
     1021 gaagaagtgt ccgacccggt aaggaatacg cccagccagc cgagaaacgg tgagaaaaac
     1081 gggaacatca cgcctgtacc tgccagtacc agcgccagcg tggtggacat gccggaatag
     1141 ttggtgacga aggcgaatgc cagcaccatg ccaatcgaca atatcggcca cttcagacta
     1201 attagcattt cggcaaagac gccaatccct ttcttgatgc ccacgccgag gatgaagata
     1261 gagataatcg ccgcaataaa aatagcggtg ccgccagccg agagagggtc gaatttaaac
     1321 accgcatcca ttggcgttgg ttgggcgaca atgggtgccg ctttcaacac ttgttgatgc
     1381 aaatgaggga tctggaaatt aatcaccagt gaataaaacg cgccgcccgg agcaaataac
     1441 gctttaaacg gcttcatggt ccagatggtg accagcaccg ttaagattaa aaacggtgac
     1501 cacgcacgaa tgatttgccc cagactatat tctgaaggca cgggaccgcc agaagatggc
     1561 ttatttacca ccatcgcacc tgcggattgc cccatgctga ttgccgtttc ggtatttttc
     1621 ggccgccaga ctttaagaaa taaagcgagt gagacgatac tcaccagcgc cgaagtaata
     1681 tccggcagtt ccggaccaat atagttagag gtaaagaact gagtgacagc gaagcttccc
     1741 ccagcaacca gcgccgctgg ccacgtctct ttcacccctt tccagccgtc catcattgct
     1801 accagccaga acggcacaag aaccgacagg aacggtaact gacgtcccgc cattgcgcca
     1861 atgtggaacg gatcgattcc cgttacctga ccggcgacca gaatcggcac gcccaacgca
     1921 ccaaacgcca ccgtcgcacc aaagccagca gctccttcca gcaacgcacc aaaggagaaa
     1981 ccaatcagta acacctgcaa acgctgatcg tcggtgatgg agataaccaa gctgcggata
     2041 atatcgaact gcccgctggc aacggttaat ttatacagga acaccgccgc gacaataatc
     2101 cacgcaattg gccataatcc ataaataaag ccatagcccg cagcagcaaa tgccatatca
     2161 atcggcattt taaaggcgaa tatcgcaatc aggatagata atataagggt tattgctcca
     2221 gcgacatgtc ctttcagacg taataccgcg agtgcaacga agaagaatat tatcgggatc
     2281 agggcgacca gagcggatag ccccagtcct cccatcggca tatacatttg ggtccaggta
     2341 accatattgt tcagtctctt attatcttta tatgcttgat atacttaagg ttgtaataag
     2401 caaaagagga ctgaactgta aaatataggc gttatacttt acagcaacag tacgccgcta
     2461 acgcaattgc tacctctggc ataacaagta tatcgggtaa gggtttctgt tccgcacacg
     2521 caggcgcaga gtatcgttaa gatgtccata ttgttgtttt aggcccgcta gtaatgcgct
     2581 acgggtattt aatattgtta aaccctgata atcgctccgg ttatttccgg gataaatgta
     2641 ctaccgcagt tactatcata gccccgacaa taaaacttgc cggggctttt ttgacgctat
     2701 taatgacttt ctttttcgcg taaacgccag gcgtgtaata acggttcggt ataaccgttt
     2761 ggctgtttca cgccgaggaa gattaaatcg ctggcagctt taaaagcaca cgagttagcg
     2821 aaattccccg ccatcggacg ataagccgga tcgccagcat tttgctgatc aaccactttc
     2881 gccatattct ccagcgacgc ctgcacctgt tctttggtca gaataccgtg acgtaaccag
     2941 ttggcgatat gctggctgga gatacgcagc gttgcgcggt cttccatcag cgccacgttg
     3001 tgaatatccg gcacttttga acaaccaatc ccctgctcca cccaacgcac cacgtacccc
     3061 agaatcccct gcacgttgtt atccagctct tgttggatct cttgcgccga ccagttagcg
     3121 ttttcagcca ccggaatagt cagcagatcg tccagcagcg gttcaaattc agcactgaac
     3181 tcggtctggg caatgttggc ttgcacgctc tgtacgttgg tatggtggta gtgcagcgca
     3241 tggagcgtag cagcggttgg tgacggaacc caggctgtgt ttgccccggc acgcagttgg
     3301 tcgcccttct ggctgtacat gtctgccatc aggtccggca ttgcccacat gcctttaccg
     3361 atttgcgctt taccgcgcag cccacagaac agaccggaaa gcacgttatt acgttcgtag
     3421 gctttgatcc acggcgtcga tttcatctgg tttttacgca gcatcgggcc agcttccatc
     3481 accgaatgca tttcatcgcc ggtacggtcg aggaaaccgg tattgatgaa cgccacgcgg
     3541 ttgcgcgcct gagcgataca gctacgcaag ttcagcgagg tccggcgttc ttcatccata
     3601 atgcccattt tcagggtatt cggtgccata ccgagcattg tctcaatgcg ggtaaacagt
     3661 ttgttggcga acgccacttc ctgcggaccg tgcattttcg gtttcacaat atagacgctg
     3721 ccagtgcgcg agtttttctg cacttttaaa tcatagaggg caatcgcgcc agtcatgacg
     3781 ccatcaagaa taccttccgg gatttcattg ccttcgctgt cccaaatcac aggaatggtc
     3841 atcaaatgac ccacgttgcg gataaacagc aacgagcgtc cgtgcagaga aatttcagag
     3901 ccatcggcgg cggtgtactg acgatcgtca ttcagtttac gtacgatttg ccgaccgttt
     3961 ttctccattt tctcttgcag agtcccctgc atcaggccca gcagattgcg gtacagcagg
     4021 attttatctt ccgcatcaac cgccgcgacc gaatcttcgc agtcgagaat ggtactgata
     4081 gcagcttcga cgataacatc gttgatgtgc gccggatcgt ctttgccaat ccgcccgttg
     4141 gcatcaattt gcagttcaat atgcaggccg ttatttttca gcaaaatgca ggtcggcgca
     4201 gcggcatcgc cacggtaacc gacaaactgt gctggagtac gtaacgtggt ttctttacca
     4261 tttttcaact ggatgcgtaa ttgtttatcg accaccttaa acgccaccac atcctgatag
     4321 ctgccgtttt ccagcggtag tgattcatcg aggaaacgtc gaacccaggc gataacctgc
     4381 tcaccgcgct gcggatcgta gccactgacc atcgcccctt cctgcgggat gatgtcgctg
     4441 ccgtataacg catcgtacag tgagccccag cgagcgttcg ccgcgttcag cgcgtagcgg
     4501 gcgttcattg ccggaaccac cagctgtggc cccgcctgac tggtgatttc gctgtcaatg
     4561 cccgtggttt ccaccgtcac gcgctccggt tgcggcacca ggtagcccag ttcacgaagg
     4621 aaagatttat aggccgcttt atcttttacc ggccccggat tgctgcgatg ccactcatca
     4681 agcgccgcct gaatgcgatc gcgttctgcc agcaactgac gattttctgg tgccagatcg
     4741 tgaacgatct catcaacatt gtgccagaat gcagcagcgt ccagttccac gcccggcaaa
     4801 acttctccat ccacaaaacg tttaaaattg gcgtcaatgc gtaaacggcc ctgggttatg
     4861 gtttgactca ttgtttctct cctcgttttc gcttatttcg ccaacaccgc tgctgccact
     4921 ttcgcgacct gcgcatcctg tgctccggtt aaaccagaaa cgcccacggc accaataatt
     4981 tgcccatcaa caacaaccgg tacgccgcct tccagcgacg tcagtaacgg cgcagtcacg
     5041 aacgcggtac gtccgttgtt caccatctct tcatagccct tagtttcacg acgccccagc
     5101 gcggcggtac gcgctttctc ctgggagatg taagccgcaa tcggcgcgca atcgtccatg
     5161 cgacttaacg ccagcagatg accgccgtca tcggcaacag caatggaaac agaccagtta
     5221 tttttctgcg cctcttcctg acctgcggca ataattgcac tcgccatttg ctggctaaga
     5281 atgactttag ttttcatttt tttattcctt ttcaagggct tgttctacaa tttcaatcca
     5341 gtgacgcaca gaggtacgac cggcgctcgc cagatgcgtc tggcaaccaa tgttggcggt
     5401 gacgatcatt tccggtttgc cgctttccag cgcattcatt ttgttatccc gcagctggcg
     5461 tgccagatcg ggatgcgtta acgcatatgt tcccgctgaa ccgcagcaca gatggctgtc
     5521 gggaacgtcc gttaaggtaa atccaagacg aagtaacact ttttccactt cgccgttcag
     5581 cttttgcgca tgttgtaggg tacacgggca gtggaaggcc agttttttat cgccgcgaat
     5641 tgccagtttt tccagcggtt cctcgcgcag aagttcgact aaatcgaccg ccagttcact
     5701 gacctgacgc gctttatcgg catataacgc atcgtttttc agcatctgcc catactcttt
     5761 gacgaacgcg ccgcaaccgc tggcggtttg caaaattgcc tcggcacctg cttcaatcgc
     5821 gggccaccag gcatcaatat tattgcgcgc ccgtgccaac cctttctcct gcgcattaag
     5881 atgatagtcc accgcgccac aacagcctgc ttcgttagct gacatgacgc tgatccccag
     5941 acgatccagc actcgcgcag ttgccgcgtt ggtgttgggc gaaagcgtag gctgggcgca
     6001 gccttccagc attaaaaccc gacgcttatg gcgcagcggc ggacgcggtt tagctttcac
     6061 cgtttcagca ggcagttttg ctctgacctg ttccggtaaa aacggtcgca gcaccagccc
     6121 tacctgcgtc agcgcacgga agaccgccgg acgcggcact acctggcgca atccttcgcg
     6181 cagcattcgc tccggcagtg ggcgtttcac tttctgctcg acaatatcac gcccgatatc
     6241 cagcaaattg tgatagcgca caccagaagg acaggtggtt tcacaattgc ggcaagtgag
     6301 gcagcgatcg agatgctcct gtgttttaag cgtgacttcg ttgccttcca gcacctgttt
     6361 aatcagatag atgcgcccgc gcggcccgtc cagttcatcg cccagaagct gataggttgg
     6421 gcaggttgcg gtacaaaatc cgcagtgaac acaggcgcac aggatgctgt cggcttccag
     6481 cgcgcgttct gccgcatctc ttcggttaat cgggtttgca tagcctgctc ctcaaagttc
     6541 cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg
     6601 gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg
     6661 ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgtcat cggctgtcga
     6721 tttcagccag cgtaacgccc cgccccagtc gatcagttgc tcgccgggta aatccatcat
     6781 cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag
     6841 ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgccac ccagcagttc
     6901 acgcgctgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa
     6961 gtaacataag ccactaatgg gtaatggctg gagttgccac tcggcgattt cactcatggc
     7021 ttcttgcagg ctgatttccc gacgcaggct cagagaggca cgcggtcgcg gtaacacttt
     7081 cattgagatt tcagtgagca cgccaagaca accatagctt ccggccatta accgtgagag
     7141 atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagtgcc
     7201 ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg
     7261 ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg
     7321 ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt
     7381 tccggcacga gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca
     7441 acgcacatcc agcgtttgcc cggtgactgg gcgacctaaa aaggctttgc tattgctgcc
     7501 ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg
     7561 actgtaatca cactcgcgta gcatcaaaag cgctccagtt caggaaaagg taaatgaccg
     7621 tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca
     7681 gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca
     7741 tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca
     7801 ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccgcccag ctcttccgcg
     7861 cgggcaaatt caccaggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct
     7921 ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct
     7981 tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg
     8041 gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct
     8101 gcttcgtcct gtgccaaacg gacgtcagtc gcgcccgctt tcaacaagat gtagttaacc
     8161 cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gttcgcataa caaaatcgct
     8221 tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta
     8281 tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc aaccgcaagt
     8341 ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc
     8401 agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc
     8461 gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg
     8521 tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag
     8581 tggacgccac cggcattttc cgccacattg ccgccaattg aacaggcgat ttgtgaggaa
     8641 ggatccggcg cgtaatagag attatgcggc gcaacggcct aggagatcgc caggttacat
     8701 acgcccggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggct ctctttaaag
     8761 cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg
     8821 cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagccag aatcgctgtc
     8881 acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac
     8941 ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca
     9001 tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga
     9061 cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg ttgtgtggca
     9121 gctgattttt gcgcgctgct tctgtgaaca gttattaagc gggcttttcg ttttcgtcta
     9181 tctctttagc taccggtcag accatttttt ttccagctct gtgaccttgt cttggttaac
     9241 ttaatgttaa attgatgtaa cataatcact tgcgtgatgt gcgtgttttg cgagttaaga
     9301 acagaaaaat tggtcctacc tgtgcacgag gtccgggaat gaaagatgaa cgtcgcccta
     9361 tttgcgaagt ggttgcagag agtatcgaac ggttaattat cgacggcgta ctgaaggtcg
     9421 gtcagccgct tccctcggaa cgtcgactgt gtgaaaagct cggcttctca cgctccgcac
     9481 tgcgtgaagg gctgaccgtg ctgcgcgggc gcgggattat tgaaacggcg cagggtcgcg
     9541 attctcatgt cgcacggctt aatcgggagc aggacaccag cccgctgatc catctgttca
     9601 gtacgcagcc gcgaacgctg tacgatctgc tcgacgttcg cgcattactg gagggcgaat
     9661 cggcaaggct ggcggcaacg ctgggaacgc aggctgattt tgttgtgata acccgctgtt
     9721 atgaaaaaat gctcgccgcc agtgagaacc acaaagagat ttcgctgatc gaacatgcgc
     9781 agttggatca cgctttccat ctcgccattt gtcaggcttc tcacaatcag gtgctggtgt
     9841 ttacgctgca atcattgacc gatctgatgt ttaattcagt gtttgccagc gtaaataatc
     9901 tctaccatcg accacagcaa aaaaagcaga tcgatcgcca gcatgcgcgg atctacaacg
     9961 cggtgttgca gcggctgccg cacgtcgctc agcgcgcagc gcgcgatcat gtacggaccg
    10021 tgaaaaagaa tctccacgat atcgagctgg aaggccacca tttgattcgc tcggcggtgc
    10081 cgctggagat gaacaaggtg ggaatgtgag cagagtttta ccctatcccc aaggcgaggg
    10141 gaccgcccct gcacttaaca ggatctggtg ttaaatctgt tcatctatac cctctcccga
    10201 atgaccgccc ctgcgctaaa caggatctgg tattaaattt gcttttcata caatcggtaa
    10261 cgcttgtacg gctccgcccc aatgcgttcc agcatgttat tcatgcctgt attggtttcg
    10321 aggatccatg acatctccag cgcatcgatc ttccggcggg caaacggatc gcgtaaggct
    10381 tcaatcaata acagcgcaat caccgggccg atgcggctga actgatactc gtcgcgcacg
    10441 cccatcagcg gtactcgcgc agttcgcaca ccgctgactt tcaaacgcca cagcaatttt
    10501 gcccagccga agggaaagag cgatccgttc agatcggcaa tcgcctcgtt gatgttaggc
    10561 aagccacaat aaacgcgcag ggtgcagaat caatctcagc gatgtagatc atatcgtccg
    10621 gcaccagata tttaagttgg tcgcccatgg tcgcgaattc atgttcggta aacggcacaa
    10681 atccccagtt gtgctgccag ccagagttga aaatctcacg caggatctgc atctcttcgg
    10741 caaaccgctg acgattgatg cagcgaatgg tcaccttttt gcgcacctga tccatcagtt
    10801 tttttagcgc cggagagaaa gtgagatcgg ttcgctgcat ccaccacgcc agtaaatcaa
    10861 tgcctttgtg ataacccagt tgttcaatat gcgcggcata ccacggtttg ccgtgtggca
    10921 tcatcgcaca gggtggtgtg tcaaaacctt caatcagtaa tccgctttcc tgattgatat
    10981 tcaggctgaa aggcccgctg atcttacttg caccttgtga cttcaaccac gcttccgctg
    11041 cgccaaacaa cgcggcaaaa tcctgcggat catcaatggc gtcaatcatg ccgaaatgac
    11101 cggtatcttc accgtaacgc tcacggtgca aggtatctat ttgcgcggta atacgcccaa
    11161 ctatctgccc cgcttttttt gccacccacg cctgccagat gatatggtcc gtccccggat
    11221 ttttcgcaga caaatgctcg ttgcgttcaa tgaataaagg gggtatccag tttggatcgt
    11281 cgggataaag tgatgacgga aaagcgataa atgccttaag gtcattttta ttaattactt
    11341 tttcaatttt aatcatcagc agatcgcatt ccatatctct gtggataaca aataattaat
    11401 ggtgaaaaac aggttgcaac agcaacgagc ggcatcttaa taatcaactc aatgaataca
    11461 aacgacgaat aaatagtatt aattaatgat tatgataagg tcagaggcta cacagaaacg
    11521 cctcaatgcc ttttgtcaat ttaaggcgac cggacaaggc ttcttcttaa tatattcata
    11581 aattaaatat cattcataat aaaaacaaaa aacttaaact cattatttaa atcaatgttc
    11641 attttcacac cgatgaaaat actatctcat ttatctttta tgcctatata atccaccgca
    11701 ttattgtcat tttatttttg atatccaatt ttttcgtggt gtatatgtct aataaaatct
    11761 ttacgcattc cctacctatg cgctatgccg attttccaac gctggttgat gctttggact
    11821 acgccgctct gagtagagcc ggaatgaatt tttatgacag acgttgccaa cttgaagatc
    11881 aactggaata tcagacatta aaaacgcgtg ccgaagctgg tgcgaagcgg ttgttatcgc
    11941 tgaacctgaa aaaaggcgaa cgcgtggcgc tgattgccga aacgagtagc gggtttgtag
    12001 aggctttttt tgcctgtcag tatgctggct tagtcgccgt cccgttggcg attccaatgg
    12061 gcgtcggtca gcgggattcc tggagcgcca aactccaggg tttactggca agttgccagc
    12121 ccgcagccat tatcactggt gatgagtggt tgccactggt caatgccgcg acgcataaca
    12181 accccgaatt acatgtttta agccacgctt ggtttaaggc attaccggaa gccgatgttg
    12241 cgctccagcg tccagttccg aacgatatcg cctacctcca gtacacctcc ggcagcaccc
    12301 gttttccccg tggcgtcatt atcacccatc gcgaagtgat ggctaatcta cgtgctataa
    12361 gccacgatgg cattaaatta cgccctagcg accgctgcgt ctcctggcag cctttctacc
    12421 atgatatggg actggtcggc tttctcctgc cccccgtcgc cacgcaactt tcagtagatt
    12481 atttgcgcac tcaggatttt gccatgcgtc ctttgcaatg gcttaaattg atcagtaaaa
    12541 atcgcggcac cgtttccgtt gcgccgccgt ttggctatga attgtgccaa cgccgcgtga
    12601 atgaaaaaga tctcgctgaa ctggatcttt cctgctggcg cgtcgctggt attggtgctg
    12661 agccgatctc cgcagaacaa ctccatcaat tcgctgaatg tttccgtcag gttaactttg
    12721 acaataaaac tttcatgccg tgctacggac tggcagaaaa tgcgctggct gtcagcttct
    12781 ctgatgaagc ctccggggtt gtggttaacg aagtggatcg cgacatcctc gaatatcagg
    12841 gtaaagccgt cgcgccgggt gcagagacac gcgccgtatc gactttcgtc aactgcggca
    12901 aagcgttgcc ggaacatggt attgaaatcc gcaatgaagc aggtatgccg gtcgcggaac
    12961 gtgtggtagg ccatatttgc atctccggtc ccagtctgat gagcggttac tttggcgacc
    13021 aggtttcgca agacgagatt gccgcgacgg gctggttaga caccggcgac ctcggttatc
    13081 tgctggacgg ttatctgtat gtcaccggac gcattaaaga tctgattatt attcgtggcc
    13141 gtaatatctg gccgcaggat attgaatata tagcggaaca ggaaccggaa attcattctg
    13201 gcgatgcgat tgcttttgtt accgcccagg aaaaaatcat tttgcagatc cagtgtcgga
    13261 tcagcgacga agaacgtcgc gggcagctta tccacgcgct ggcggcacgg atccaaagcg
    13321 aatttggcgt gaccgcggct atcgatctgt tgccgcccca cagtattccc cgaacgtcct
    13381 ccggcaagcc tgcccgtgcg gaagcgaaaa aacgttatca gaaggcttat gctgccagtc
    13441 ttaatgtgca ggaatccctg gcataaatca aactgtcgcg gtgacgggcg ctaccgggtt
    13501 tatcggtaaa tatattatcg ataacctgct cgcccgcggc tttcacgttc gcgcattgac
    13561 gcgtactgcc cgcgctcacg tcaatgataa tctcacctgg gtgcgcggtt cgctggaaga
    13621 tactcattca cttagcgagc tggttgccgg agccagcgta gtggtccatt gcgccgggca
    13681 agtgcgcggg cacaaagaag agattttcac ccgctgtaac gttgacggca gcctgcgcct
    13741 gatgcaagca gcaaaagaga gcggcttttg ccaacgtttt ctgtttatct cttcgctggc
    13801 ggcgcgccat cccgagctct cctggtatgc aaaatccaaa tacgtcgccg agcaacgact
    13861 ggcagcaatg gcggacgaaa ttacgctggg cgtttttcgc ccgacagccg tgtatggtcc
    13921 cggcgataaa gagttaaaac cgctgtttga ctggatgcta cgcggcctgc tgccacgact
    13981 tggtgcgcca gatacacaac tctcttttct tcacgtcacc gattttgcgc aagcagtggg
    14041 tcagtggtta agcgccgaaa ctgtacagac gcaaacctat gaattatgcg atggcgtcgc
    14101 cggtggctat gactggcaac gcgtacgaca gcttgccgcc aaagcccgtt gtggttccgt
    14161 gcgaatggtt gacattcctc tgccggtact cacaggcctt gcggatatca gtaccgcgtt
    14221 gagtcgcctg gcaggtaaag aacctatgct gacccgctcg aaaattcgtg aattaaccca
    14281 cgccgactgg tcggcaagta ataaccgtat ttctgaagat atcaattggt ttcccgggat
    14341 tagcctggaa cacgcattac gcaacgggct attttgattg tcttgtttta aaggtgtgtt
    14401 aacgtttcta tggtaaatcg tgaaatagta atggattaca tcctttcctg tttacaggat
    14461 ttggtcgaaa acggtgtgga aattaagccc gacagcgatc tcgtcaacga tcttggcctg
    14521 gagtcgatca aagtgatgga tctcttaatg atgctggaag acagattcga tatttccatt
    14581 cctattaata ttctgctgga tgtcaaaacc cctgcgcaat taatggaaac tctacttcca
    14641 tggctggaga ataaataatg gggctatacg ataaatatgc gcgccttgct ggcgagcgtc
    14701 tgcaatttag tgacaacggg ttaacaccgt ttggcacctg tattgatgaa gtgtattccg
    14761 ccaccgaagg ccggatcggc aataaaaaag tgattctggc cgggaccaat aattatctcg
    14821 gtttaacctt taatcatgat gccatcagcg aaggtcaggc cgcgttagcg gcccaaggca
    14881 ccggcaccac cggttctcgt atggcaaacg gcagttatgc gccgcacctg gcgttagaga
    14941 aagagattgc cgagtttttc aatctcccta ccgctatcgt ttttcaaccg gttataccgc
    15001 taatttaggc gtcatcagcg cacttgccga ccataacgcc gtggtgttac tggatgccga
    15061 cagccacgcc agtatttatg acgcctgttc tctcggtggc gcggaaatta ttcgtttccg
    15121 ccataacgat gctaaagatc tggaacggcg catggttcgc ctcggtgaac gggcgaaaga
    15181 ggcgattatc atcgttgagg gcatctacag catgctcggt gacgttgcgc cgctggcgga
    15241 aattgttgat atcaaacgtc gtctcggtgg ttatttgatt gtcgatgaag cgcattcgtt
    15301 tggcgtgtta ggcgcaacgg ggcgcggact ggctgaagct gtcggcgttg aagatgatgt
    15361 ggatattatc gttggtactt tcagtaaaag tctggcctcg attggcggtt ttgctgtcgg
    15421 ttcggaagcg atggaagtgc tgcgctacgg cagccgcccg tatattttta ccgcctctcc
    15481 ttctccgtcc tgcattgcga ccgtgcgctc gtcgttaaga actattgcca cacaaccaga
    15541 gctgcggcaa aagctgatgg ataacgccaa ccatctgtat gacggtctgc aaaaactggg
    15601 ttatgagtta agttcacata ttagcccggt agtgccagta ataatcggtt cgaaagagga
    15661 aggtttacgc atctggcgcg agcttatctc gccgggcgtg tatgtgaatc ttatcctgcc
    15721 gcctgccgcg cctgcaggca tcacgctgct gcgctgtagc gtaaatgctg cccacagtcg
    15781 tgagcaaatc gatgccatca ttcaggcatt tgctactctg aagcagtaat gtgagcccgc
    15841 cgtggtgttt gcgccacggc gggtttaatg ttgtctgtca taccaataca ccaggctcaa
    15901 cgccaccaac acaaacgcta ccggtaaacc gagcgcggta acaggtgcgc ttaacgcaaa
    15961 tagcaatccc agattaacca tgatttgata gctgatccag gtgagtaagc ccacgatgac
    16021 gcctacagcg agacggctac ccatcccagg cgaacgcggg gcactaaagg taaacggcac
    16081 agccagcaaa atcatcgcca gggtcaggat gggttgcccc agtttttccc acagtgcaag
    16141 gcggtattcg ctgctgggtt gcccggtttc ctgcaaataa tggatgtaat gattaagctg
    16201 acggacagaa aaagtattcc ccggcattga taactcttca agattcatac tggtgaaggc
    16261 cgattgccag gcaagattat ctaatgtttc cagcgtttct ttgccattca gccatttttt
    16321 atgattcaca ccatgcagga cccacgtttt gtcgtcttca atggtggcac tgcgtgcata
    16381 aatgtaggat tcaagcgaga gatcgtcgcg ataatgaaat atctccacgc ccaccggctg
    16441 gccctgctcg ttcagcgatt tcaccgtcac aaattcatta ccacgcctgg cccacagcat
    16501 attgccggta atgtcatcgt cctcccccaa cgcggtggcg gtcgatttga tttgcaacgc
    16561 ctgttgctgc aatggcgacg ccacccactc atctatcgcg cctaacgaaa cagtccacaa
    16621 tatcccggca accagcgcca ccagtgcaat acggaagata gaaaaccccg tgctgcgaat
    16681 ggcggtaagc tcactgtttt tcgataactg ccccagtcca acaataccac ccaataacgc
    16741 gataaacggc gaaagttcga ccagtgtgcg cggtaaggtc attagcacca ccagcaccgc
    16801 ctgagtccag cgataaccgc ccgggctgac accatccagt tcgttaatca ggttgaaggt
    16861 ggtaaaaagc ggcagcaata gccctgcagc ggcggcaaaa ccgagaaaga gatgacggat
    16921 taaatagcga ctgaaaacat tcatcgggaa aatttctgca gcaaagagaa gtcacgtgcg
    16981 accagtatca gcaggcccat tagcatgagt ccgggcacta accacaaacc aggaatgagg
    17041 ggaatcgcgc cgttagccac cagtgtacgg cagacgtcgc cgccataaaa aatggcaata
    17101 aacagcaacg tcagtggcaa taacgttgaa aatcgccctt gccgcggttt tacccgactt
    17161 aatgaaatgg ccagcagcgc catcaacaat gcggtcaggc cacggctttg tcgccattgc
    17221 agttcggcat gatcggcagg aaaggcggag cgcgccagct ccgtgaccga tttcgctttg
    17281 cgtttgacgt tcgggctttg atccagcgga ttcagatgta attgcagatt acggtagatc
    17341 tgttcgttgt cgtcacgccc ctgatgatcg agaagatagg cagtgccgtt gtgcaacata
    17401 acggtcggtt tctcgggtga tgggtcaacc acatcaaccg aaccggcgcg gaaaatacgg
    17461 gttcgattgg cagtagaagt ataaatcagc gcgtcagtca ggcgattatt atcctgatca
    17521 accgtctgcg aaaggatcat tcgtccgtta tcgttagtgt taaatttctt tgcccgcaac
    17581 tggcgaacat ccagctccga ctgcgactgc tgctccagtt gataaatctg cgcataagcc
    17641 cacggacgac catacatcga cagaagggtg acaaaaatac ttagcggtat cgccagataa
    17701 agaacggctt tgtacaagcg tcccggactg ccccccgccg cagagatagc ggtaatttcc
    17761 gagtcggtat acatctgccc tagcgtcacg ccaactgaca catacagacc aacaggtaac
    17821 aacatctcca gtgcaatcag tactttgtag aaaacgatat ccagcacaac atcaagagct
    17881 aatgtcccat ttgccgcttc ggtcagataa cgctgtgcgg agtagctggc gaaaataaag
    17941 atcagaaaac cgacgataat cagcaccaga cggcgcgtac cacgcatgat gtagtgttca
    18001 accagcttca tctcactaac gccctcaatc ctttgagttt tttattcatt gatcagtttc
    18061 tgcgccaacg caatgacatg atctacctct tccggcgtca ggctgccgtc ctttgcccac
    18121 tgaatttgcc cggctttatt cagcaccaga atggttgaac tctgctccgg caagcgccag
    18181 gcaacgcgtc cctgtccatt gccgtcaata acgaattgcg cccacggata gcggcgttta
    18241 tttttttcga ttttgccaac aacaaaatag ccagtaccaa agatggcatc atcggtgttc
    18301 acaatggtgg tgggctgaaa acgatcctgc ggaaagtttg ccgcttcaac cgctttaatt
    18361 aacagtgagt tctttttctt cgccgactta cgcccggcga tatactgaat aatgcggact
    18421 tttcccgcta attgtgtgct gttccacttt tggtagtgaa tttcgtcgtc gctattcaga
    18481 agcagctcgc cgccttcctg gatataaaca ggggtaaccg ttttgccagt gacgaaattg
    18541 tgtgcctgca cacccggggc taagagcatg ataatcaacg caataatcaa acgagatgac
    18601 atcattgctc cggctatagc ggaaaacgcg aaatcagcag ctctaagcgc tgacaaatat
    18661 cgctatccag cgccaacggt gcataatacg actggcggtt ttccatatca tccgccacca
    18721 tgtcgtcgtg tttatctccc atcaggccat taatggcggc gaggaaacgg gaaatctgta
    18781 acgcctcttt attttcagga tcaagcgatc ttaaggtgtc gtaataagaa acctgctggc
    18841 tgatgatttt tcgcacatca tgaaattccc tgccggttag tcgcggtttg gcaataatct
    18901 ctttcagggt atgcatttgc ttttgccgat aggcaataaa actgcttacg ctttctaact
    18961 ggctatggcg taatgaatag ttcaccaacg accagaccgg agagcttaaa taaatccgta
    19021 acaggttgcc gtaatacgac acaatatttt tcttaccgtt acgaaagccg tcctgaaaac
    19081 gccccagaaa aacggtcgtt ttgccgaata aaaatccggc ctgatgcttc tttaaataca
    19141 gccgttgcgc aaaacgcagg tgcttgtagc gcgctcgcat gtatttaaat tgtttgcgct
    19201 catcagcagt cagctcatcg cctttggctt gtttgcgaac taaacgcagt aattcctgac
    19261 gatcaacgcc attgagccag aattgcaggc ataacgcgta attatcatga atgatttcgg
    19321 tggtacaact tagtgcaata gtcggcggca aactaacctg tgcatcaacg acatcatctt
    19381 cttcaatcgc aacaaataga cgctcgctcg cctcaagcgg gaaatatttt ctcatcatca
    19441 ggatttctct ccggctgcgt tataaagcaa ggaatggcgc tgaaaaaatg cctttaaggt
    19501 tggaattaac gagagcgcca cgtccagaca ttgcccaccg aagtaagcaa acgctgctcc
    19561 cagcacacca aacagatgac agaacatgaa cagcaatacg atgtagccga ttgaggcgat
    19621 ggtttgcgcc acgagaaaag cgcgctgttt tcccgccatc aatagcaatg actcctgcgg
    19681 gaagcccagc atcgaaatca caattgcgcc cagcatcacc tgaatcaggt catacgcttc
    19741 gagatactta acgccaaaca ccagcgaaat gagcggcttg ccgacgatca acaccgcgag
    19801 agccaccaga ataccgattc cgcccgccag taaaccggac ttcacaccta acaaccacgg
    19861 tctggtggtg cgcggatcta aacgcatcac ctctgggtaa aaacttttac ccagcaaacc
    19921 cgctggcgtt ccggcggcgt cgaagaatgt catggcgatt ttaaataacc cggcggcagc
    19981 gggtcctaac acgatcccca ccaacactgt gctgcacgag ttacgcgccg accagatgga
    20041 gtgggcaatg ttggttgacc agacaaaact ccacgcgcct ttaatgtgtc gggcagactc
    20101 aaacagattc aatttgaagg cgttatggat atttctacgg cgtaattcgc gcgcggcaaa
    20161 ccaccagtac atggtgccgc caaccagatt cgacacgtac caggcaataa caaaacccgc
    20221 aaaaccaaag tcaaaatacc aggctgcgac gctccccgct gcgcgcagaa aaggtttcgt
    20281 cgcctgctgt acagcaatca aatcaaagcg atctaccgca cgcagaatgc cggtcggcgt
    20341 ggaggaagcc attgaaggaa tgagcgtgca atacagcgcc gccagccaaa agctttggtc
    20401 atccagacct aatgaatggg aaaggaatgg cagtaaggca atgccgccga caatcgccac
    20461 cgcgccgctg acgatatcca gcgagaagga aaatgagacg acattgcgaa attgctgcgg
    20521 attattgttg gtcaaggctg gcgttccgta ctgaaccacc agctgccatg tctgaaactt
    20581 aataaaatcg ctgatcgact tggcgtacga ttgcacaatc accagtacac caaacatggc
    20641 gggcgtcatc cctttaccgg cacacgagag cgccaacagc cccagcaagg cgctctcgac
    20701 attactggag cctaaccagg cgctattgcg aatcatggtg cgaaacgcgc catctgcaaa
    20761 ccaatgtttg atgttaaaac ccgccaattc agcctgacct tatcgttgat agtaaaaaat
    20821 atcccgccag ccttaagtta aacttcggcg gtcagaaacg atggcaacca gagaaaccgc
    20881 cttctgtacc tgttccagca cttcgctgta gggcgctctg gaatcaatct caagaatttt
    20941 tgtgccgtta tagccaatcg tcgacatgac gccgattttg tcctgcagct cggcatactc
    21001 atggtcaggc ttgcgggaga tggcagtatc aatatcaatc cccaaacgaa tgattaattc
    21061 aggacggtat tgcgccattt tttggtataa actccgttcg cgctgcgcca gaaacatgct
    21121 gattttcccg gtcgcacgtt cgacgccgat ccccggtccg tcataataaa agcccgaaat
    21181 ttcagcctgc gggaagcgat cgctgaccac cagaacgcca ctttgcgcca gtcgctgaac
    21241 cttgcgtaga ttcgccattc ttcgcagcga gaagcagtac ataatcaccg ccgcccatag
    21301 cgccggagat ttggttttca tgctttgggt tttcgaggat ttggccgcca atcgccgttc
    21361 cagccagacg ccaaccaacg gcaatcgttt gattttgtcg ccgtcttcgc cggagagcag
    21421 ccccagatag cggcgctcgg tttgccagtg ttgttgcagc gatttcacca ggtcggtggt
    21481 cagcgtggat ttaccggtgc catcacaccc gaccaccgca atcagccccg gaatgtagtt
    21541 gggctgcggc gcggttgtac tattaacagt ttgtgtttgt agtgcatcca ttcgttaaat
    21601 catcccctat ccattttggt gatcaggaaa gcgcggcgtg aattgcgcgt agtgacgctt
    21661 gcataatttc atcggctggt tgccgaccat ccagttcaag gatcttggcg ccattgaatg
    21721 tcagttgcgg cgtaacggcg attttttcct gtaacgctgc cagttggtgg tcaggtttac
    21781 gcgcaaacgc ggtttgttca tcaatgccaa gacgaatcaa caatacgggt aaataagacg
    21841 ccatccattg gtacagcttc aactcgcgct gccttaacat ttttatccaa ccgttaccgc
    21901 ccgtggtttt tgccaattgc gggccatcaa agcgaaaacc cggcacttca acctgcgggt
    21961 agcggtcggt gatgagcaga tagccttgct ggcttttaca caacattttg cgaaacttgt
    22021 acgcccgcca gcaggaaagc agatagataa ccagcgcagt aatattgcca ggcggagtta
    22081 agggcttttc gtgtacatgt gccgctttac ttcgcagata acacccacaa ggtgcgccaa
    22141 taacagggag ctgtgaaatc cattcgccaa ttcgcccgga cgattgcccg agataaatgt
    22201 gttctgttgg cattcttgct gccagttcat ttaccaggct tgccgtgagg gtcgatttac
    22261 ctgaaccatc acatcctaca atggcaataa cacgcactgg cgtggaatta attattgaca
    22321 tattacgttg attcacgaaa aacccggcag taaataatgt attgaatatt acgtggtccg
    22381 tgctcagaat atccgttcaa ccttattgtg acaatggcta attgtgacat tgacctgaat
    22441 tacgctttca taaaaacata ttaaccaaat aaatattttt aatggatatt taaattaaag
    22501 gatatattca tgcagtcaat aacacctcca ttaattgccg ttattggtag cgatggttca
    22561 ggcaagtcaa cggtgtgtga acatcttatt accgttgtcg aaaaatatgg tgctgccgaa
    22621 agagttcatt taggaaaaca ggccggaaat gtcggtcgtg cagtgacaaa attacctttg
    22681 atgggaaaat ccttacataa aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg
    22741 cctggaccag ttccggcgct ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt
    22801 cgtcatatgc ttgcctgtcg tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag
    22861 gaccaaattc ctggcgctta cgatggtacg gtgttcccac ctaacgttga aggtggtcgt
    22921 tttgtctcat ggctggcaag ccaggaacgt aaagcgtttc actggatggc gagccataag
    22981 cctgatctgg tcatcaaact caatgttgac cttgaagttg cctgtgcacg taaacccgac
    23041 cataaacggg aatcgctggc gaggaagatt gccataacgc cacagttaac ctttggtggt
    23101 gcacaactgg ttgatatcga tgccaatcag ccactggaac aggtgttggt tgatgcagaa
    23161 aaagcgatta cggattttat gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc
    23221 gatcgccagg accgggcatt ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag
    23281 cccaccagaa agaaaaattg ccgccggtaa agtaaacacc cacgccatca ggatgctggt
    23341 taccgtttta cgctgtaacc caccgccgtc caccaccatc gtccctgcta ccgcagacga
    23401 gaggacgtgt gttgtggaga caggcatccc aatataactg gcaaggccga tagacactgc
    23461 cgccgtcatt tgtgccgcca tgccttgcgc atacgtcatg ccgcgcttac caatcttctc
    23521 accgatggtc atcgctacac gacgccagcc aatcatggtg ccaatgccga gcgccagtgc
    23581 taccgccatg atgatccaca ccggagcgta ctcaatggtg cttaacatat cgctgcgcag
    23641 ttttttcagc aggttctggt cttctttact gacgcctggc agtttcgcca gcttcgcgga
    23701 ggtatcggag atgcacagca taatgcggcg cagctggctg cgctgactca cgcttaacgg
    23761 ctcgtaactt tccatattgc ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt
    23821 atttgccgga tgacagtgaa actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa
    23881 tggaggttcc atcgcaatca acttctgcgg cagttcagga tgctgttgca ggtagtgttc
    23941 gaagttggta acggcatcgc gggtacgggt aatttcatag ccggacgcat tcatattgac
    24001 gacgaagcca gcaggggcaa tccccaccag taccagcatt accaggccga tccctttctg
    24061 cccgtcgttc gcgccgtgcg aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg
    24121 cgtccagaat ggcggtttac gtttgccctt tttctttttg cgatcttccg gaatgcggtg
    24181 aatacggtca cgctttttcg tcccactcca gaaacggcgc agcaggaaaa tcagaccacc
    24241 cgcaatgacc aggccgacga taggggaaac aatcagcgag gagaaaattt tggttacttc
    24301 acgcaggttt aacgcatcca tcaccgatga gccggttaac agcgcgttgg ttaaaccgat
    24361 gccgataatc gcaccaatca aggtgtgcga actggaggcc ggtaaaccaa agaaccacgt
    24421 tcccaggttc cagataatcg ccgccagcag catggaaaag accatcgcca aaccgtgagt
    24481 tgaccccata ttcagcaaca aatcgattgg taacatatgg acaatggcat aggcaacgct
    24541 aagtccgccc aataacacgc caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg
    24601 tggctgcatg gcacgagtat aaataacggt tgccaccgca ttcgccgtgt catgaaaacc
    24661 attgattgct tcgtagaaca acacaaatgc cagagcaagt aataacaaaa gccccgtata
    24721 tatatcaagg ccaacaaata aatttagcat atattagatt acgccatttt gaatttacga
    24781 acggacgcat tatcagtgac tttaacggca tgggcaaagt gaaatatcat tttttaacga
    24841 taaaaagagt gttatttctt gtgtatcaga caataagcat tcattcaaaa cctttagaaa
    24901 taatcaaaac acaggtattc cactggttaa tatattcaca tatgaaatga ataaatattg
    24961 gaatatataa atattgaata ttttgattaa tccccgtact aattattctt cataatcagt
    25021 acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa tatcactctc
    25081 ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt tgccgccaac
    25141 ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc acagttgctg
    25201 atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct cttcatgatg
    25261 gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg cgtaacctgt
    25321 tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa
    25381 cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc acagcggctc
    25441 aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt tttgcggatg
    25501 accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa tctgatcaaa
    25561 cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt ccccatcaat
    25621 cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac gcgtttcaaa
    25681 gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct cgatatcttt
    25741 gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac cagccagttc
    25801 gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct ccgcccaacg
    25861 ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt attcgtaaac
    25921 cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat ggtgacggcg
    25981 acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga acagcgccag
    26041 caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt
    26101 ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat gatacggatc
    26161 ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat ctttttcatc
    26221 cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga ttttcagcag
    26281 ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct ggatcatcca
    26341 gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt tttcgaccac
    26401 catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa tcacgttctg
    26461 ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc
    26521 tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg cacgcggcga
    26581 gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc gcaggaagcg
    26641 cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt aattcagaaa
    26701 gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt cgtcgtcgat
    26761 atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac tgtaatctga
    26821 agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg gggcatcctg
    26881 gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc agttggcgta
    26941 attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca gactaatcat
    27001 acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg
    27061 acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac agacaatact
    27121 tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc
    27181 aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa acatccattg
    27241 caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct tgaggagcta
    27301 ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat tggcgatggc
    27361 gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc
    27421 gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct gctttatctg
    27481 gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga aacgttgaac
    27541 tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg tcacttttac
    27601 cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga agccaagcgc
    27661 ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg cgatgagtac
    27721 accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat
    27781 gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa
    27841 gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa cgggccgctg
    27901 aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac agaagataag
    27961 cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat aaggcgttca
    28021 cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct
    28081 acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat
    28141 gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg taccagcacc
    28201 cactggccca gcagatcggc agggttacct tcgcaaatca gggcgatatt cacgtcgcgc
    28261 ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc
    28321 tggcctggaa cgccaataca cattgttaac tccggttatt tcgattgccg gacaaacgcg
    28381 aaaaagaacc tttagcgggt gttccctctc accctaaccc tctccccgga ggggcgaggg
    28441 gaccgtccgt gcgcttttta ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac
    28501 gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc
    28561 atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaggcct gggcgggttt
    28621 atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact
    28681 aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt
    28741 gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca
    28801 aagcgacaac tcatgcattt tcgctcctcc tgcggccaaa cagcagggcg cgacgccctg
    28861 cgtgtggtac atccggattg gtgactggca acgaaaggat cattcgtgcg cagtcatccg
    28921 tcaggcgttg gccttcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg
    28981 agaggtattg cgaaacaccg tccagttcgc caacggtaaa ggtcatggtg ccatacggca
    29041 gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga
    29101 agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca
    29161 gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg
    29221 aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac
    29281 ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc
    29341 agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag
    29401 ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac
    29461 tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg cggagagatt
    29521 ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggagtg
    29581 ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca
    29641 agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat
    29701 cgttgctcta acgcttcgac aatccgcacg ccgatggctt catcggtcag caaaatattg
    29761 ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt
    29821 cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga atggtacgca
    29881 ccacttccag cggtttattc ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag
    29941 gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga acaaccgcct
    30001 ggtagttgct gatgataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg
    30061 cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggc ttcacaaagg
    30121 tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattggttt tgcaggatat
    30181 cctgcaattc acagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct
    30241 gtgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt
    30301 tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc agcggaccca
    30361 cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag
    30421 accaaccatc ataagccgga atggtggtac cttcccacgg cgcctgtggc gcttcgtctt
    30481 tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag
    30541 aagtgatcgg acgatacgag gacagatccg cattctcaat gtagcctccc gggaacagga
    30601 agctgccgtt tttgctgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt
    30661 taccgcgcgt cagccattcc gggtagaacg cggcgataac cgcggtatca accttataaa
    30721 cctgctcaac aaagtcgctc agtttgtcga tgaaagactt gatgtacatc aggcgctcaa
    30781 ggttcaacac gcccaacccg tcgaggttga ttgggttcgc gacaccaccc actgccaggt
    30841 tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc
    30901 actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca
    30961 tcgccgggtg accccagtag ccattggcga aaatacccaa ctgaccgctg gcaaccagat
    31021 ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc caggtcgaaa
    31081 cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca
    31141 cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag
    31201 ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt
    31261 ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca
    31321 caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca
    31381 tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta
    31441 aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc
    31501 ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag
    31561 tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca
    31621 ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accaacgaat aggtcagacg
    31681 ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatgc gggaatcatt
    31741 acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca
    31801 gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag
    31861 ctcgccaaag cgcagcccga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt
    31921 gacgaacagg ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagtgaacc
    31981 ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa
    32041 catttcatag ctctgccaga gcggatgcac cttgtagccc gccgagatca tcagcgaccc
    32101 cattgaagac tggtgcatgg tcggcagcag cgcaccgagc gcgatgatga agaacatcac
    32161 cttgttcaga cgttgtagcg acaccttcca gcccagacgt tcaaacagtg ccggagcaaa
    32221 ctccagagcc atcacgccga tgtagatggt catacagacc gccgtctcga acagtaccga
    32281 gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc
    32341 gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg
    32401 caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc
    32461 gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa
    32521 gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag
    32581 catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga ttttgccgcc
    32641 cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt
    32701 tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat
    32761 acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca
    32821 ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt
    32881 tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg tcgccagatt
    32941 tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg
    33001 cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta
    33061 cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc
    33121 acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg
    33181 cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt
    33241 tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt
    33301 gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgacccctg
    33361 tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg
    33421 accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga
    33481 cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa
    33541 ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg
    33601 ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc
    33661 agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc
    33721 aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc
    33781 tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg
    33841 atggatgcct ttatggaagc cgataccttt ttcgttacag ccatagcacg ggtgaccaat
    33901 cgccaccggc cacacaccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt
    33961 ttctggccct ttacagccga ggtggtacag gcaccagcct tcgcggtggc cttcatcacc
    34021 gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat
    34081 cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta
    34141 agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat
    34201 attgataacg gttttgcctg gcagaacttc ttgcaagctg actgcgccag ttgggttaac
    34261 tccagctgcg gcaacaccgc cccacgcgga gcaggaacca atagcgataa tggctgctga
    34321 gccttccgcc gctttgcgga tgtgatccac aatcggctca ccggcaacca tgcaataaat
    34381 accgttatct tttaatggga tggaaccatc caccactaac acatactgcc ctttgtactt
    34441 ctcgagagcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc
    34501 gtgatattcc agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg
    34561 aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg
    34621 ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt
    34681 ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat
    34741 gagggtgtta tctccagtca tttatagtta ttccgttgcg aggacctggc atatattttg
    34801 cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt
    34861 atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt
    34921 aattaaagca agataatacg tatgtttgat caattttcga ggcaataacc ggccataaaa
    34981 aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc
    35041 agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc
    35101 ttttcgtaca tccggtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat
    35161 tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta
    35221 ccaaaatggt tattcatcgt tcacctcatt cgttatatat cgccatatat agcgatattt
    35281 taggcaaaga acgacttttg cgcacgctat tttttcatac ttgtttaaga cgtaatcccg
    35341 taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc
    35401 aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac
    35461 ccccggatag atataagcct cataaacctt attattgtct ttcaacgccg cctcgtaagg
    35521 aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc
    35581 aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag
    35641 ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat
    35701 acccacttta cctgtcgctt gcggatagcg ttgcataaac tcaattgcgg caaagaaatc
    35761 attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat catcatttcc
    35821 cggataacct ccaacggaac ttaatccgtc aggtgccagg gcgatatacc ccgccttcgc
    35881 cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat gcaccacccc
    35941 cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc
    36001 gtgaccattt ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc
    36061 tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat
    36121 cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg
    36181 aacgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg
    36241 catcgttgca ttcctcaact ggtgtttttt taagcaaagc ataagcgcgt gtttttgccc
    36301 agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag
    36361 aaacattgtt tcgaaattga acggaggaaa ggagaggtca tggtctggtt agcgaatccc
    36421 gaacgttacg ggcagatgca gtaccgctat tgcggaaaaa gtggtttacg cctgcccgca
    36481 ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgca
    36541 atcctgcgta aagcgtttga tttaggcatt acgcactttg atttagccaa caattacggg
    36601 ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgcgagga ttttgctgct
    36661 tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc cggcccgtac
    36721 ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg
    36781 gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa
    36841 gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc
    36901 tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt
    36961 ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc
    37021 ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag
    37081 ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa
    37141 gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg
    37201 cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc
    37261 tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa
    37321 ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag
    37381 attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc cgataaatga
    37441 cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag
    37501 tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga taatgacgta
    37561 ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat
    37621 ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt
    37681 ccccagccac ttcagcttct ctttgttctc ggagatatcc agctgtgaga cgaaattctc
    37741 ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga
    37801 cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat
    37861 aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac
    37921 gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc
    37981 ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc
    38041 aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt
    38101 tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg aagggagaaa
    38161 aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat atcccaaaca
    38221 gaaacaaccg acgccaggca tccaggcgaa gatgacaccg ataccggatt gcggcgagaa
    38281 aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc
    38341 cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag
    38401 ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg
    38461 acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt
    38521 tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca
    38581 ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat
    38641 taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc
    38701 aagtgtcatc accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta
    38761 tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga
    38821 gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc
    38881 cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga tgaagcgtgc
    38941 ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta
    39001 cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg
    39061 gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc
    39121 cttatccgcc tacaaacgca tgaagattca acatgttgca atagattttg taggcctgat
    39181 aagcgtagcg catcaggcat tgagcaccta atgcctgatg cgtcgtgaac gccttatccg
    39241 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    39301 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    39361 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    39421 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    39481 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    39541 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    39601 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    39661 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    39721 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    39781 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    39841 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    39901 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    39961 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    40021 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    40081 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    40141 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    40201 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    40261 gcggcgatat cgttggcctg gtttaaggaa cgtgcttcag ccagcagttg ctgctcgcgc
    40321 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaggtg
    40381 actacagagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtactga
    40441 tacatacccc agacggaaag gtccgtctgc attaaattat tacccactgt gtatctccag
    40501 gacgcaagtc acaaaatctg cgcataataa tatcaaaacg acgtcgaatt gatagtcgtt
    40561 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    40621 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    40681 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgacat
    40741 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    40801 tggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtatttgaga gtatggaagc
    40861 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    40921 gttaacccat ttttccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgc
    40981 gctatttccc tgcggggcgg cagcggttgc taattcgatt cttgcttttg tcgaacaggg
    41041 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    41101 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    41161 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccgggtt ccatcactat
    41221 ggaagtccac gacgttccgg cgattgttgc cgccgtgcgc agtgtggtgc cggatgccat
    41281 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    41341 cgatgtttct attcaggccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    41401 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaatgcct atctgatggg
    41461 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    41521 gcgtttgcgt caacatcatg aaagcagtct gaaagtggcc gaatggctgg cagaacatcc
    41581 gcaagttgcg cgagttaacc accctgccct gcctggcagt aaaggacacg aattctggaa
    41641 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcaatga
    41701 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    41761 cgggtatgaa tcgttgatcc tggcaaatca accagaacat atcgccgcca ttcgcccaca
    41821 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    41881 cgatctgatt gccgatctgg acgccggttt tgcacggatt gtataacatt gccacttttg
    41941 gacaattttg cagacatttt attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    42001 gtcccggacg attcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    42061 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    42121 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    42181 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    42241 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    42301 caagtctggg ctgctggcta agttatattc aggggagctg gttagggaat accaaaacgg
    42361 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    42421 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    42481 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    42541 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    42601 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    42661 tgttaaccgc tggcttgtta ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg
    42721 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    42781 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    42841 ctaaattgcg atgcgctttc atagccgacg cgcatcgctg ctgcgctggc cttcatgccg
    42901 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    42961 gaggtgctgg tgacagactt aaaattatgg tggaacgccg atacgctcat gttggcttct
    43021 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaattcg tttcagcacg
    43081 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    43141 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    43201 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    43261 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    43321 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa ccctgccagc
    43381 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    43441 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    43501 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    43561 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    43621 ttaactttat ccgccagcaa gcgacaaatc tcttcacgtt tcatatcgcg taatttctta
    43681 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    43741 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    43801 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    43861 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    43921 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggtgg cggcagcgtg
    43981 aaaaaaaccg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    44041 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    44101 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    44161 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    44221 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    44281 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    44341 gcgttccatt ctgcacatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    44401 accctgccgc cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa
    44461 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    44521 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    44581 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcac tggcgtaccg
    44641 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    44701 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    44761 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    44821 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    44881 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    44941 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    45001 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    45061 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    45121 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    45181 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    45241 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    45301 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    45361 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    45421 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    45481 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    45541 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    45601 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    45661 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    45721 agaaagtcat tcgcgatctg gcagataaat acggcaaaac cccggcgcag attgttatcc
    45781 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    45841 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc aaaattgcaa
    45901 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacgtgcaa
    45961 attctcccgg tggcggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    46021 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    46081 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    46141 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    46201 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    46261 aaatcagatg ccagtacgcc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    46321 cgtattcaac aggcgcttac cctgaacgac aatcttacac taccgggcaa tattggctgg
    46381 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgtt agaaagcgta
    46441 gcgataaccc aaactgacgt cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    46501 gatgataaag aaaagaccac acagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    46561 attgatgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    46621 aaaacgctgg ccgctttagc ttcattgcag aaagaacagc cagaatcctt tgttgctgaa
    46681 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    46741 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    46801 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    46861 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    46921 catgttcgtt ttacgctgac caatggcaac aatgaagagc aagagctggt tttacagcgg
    46981 cgcgaaggta agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    47041 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    47101 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    47161 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    47221 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatatg aaagcactac
    47281 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgcac
    47341 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    47401 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    47461 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    47521 gtctgacgct gagtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    47581 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    47641 ctgccaatca gatgcttttt gcccatcgct tccagcgcct gacggattaa cggccagttt
    47701 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    47761 acgaagacgc cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    47821 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    47881 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    47941 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    48001 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    48061 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    48121 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgataagg
    48181 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    48241 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    48301 tcagcgcgtg gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg
    48361 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    48421 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    48481 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    48541 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    48601 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    48661 tgtttttgca tcaatgcgcg ggcgcagcct gggttggttt cgtggtgcag aatgcgcgaa
    48721 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caatacgtag
    48781 gttttttccc acggtttctg gcgcggtggc tgcacgatta cagctttcgc ttcctgcttt
    48841 ttcggcgcaa caggtttgtt atccgcacac ggcaaatctt cgccatacgg atgcgggatt
    48901 ggggcgattt tccctggggt atcaagacgg gtggaatcca caccgctcca gccaggcagc
    48961 gcctctttta cgataattgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    49021 atcgccagac gatgcactac ttccaccagc ggacgttcac cgttaccaaa catcagcatg
    49081 tcggctttcg aatctaccag aacggaacgg cgcacggtat cggaccagta atcataatgc
    49141 gcggtacggc gcaggctagc ctcaataccg ccgaggatca ccggcacatc tttccacgcc
    49201 tctttacaac gctgggtata aaccagcgtg gcgcgatccg gtcgcttacc cgcgacatta
    49261 tctggcgtgt aggcatcatc atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc
    49321 gaatccatgt tgccagcagt aacaccaaaa aacagattcg gtttacccag acgcataaag
    49381 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgcgcttcc
    49441 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    49501 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    49561 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    49621 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    49681 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aggtattcca tactcgccct
    49741 cctcgggcga gtatgaagat tacggtaccg gattgaccaa cagttgccca atcgagccac
    49801 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    49861 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    49921 tgtcttccgg gaacggcatc gcgccattca cgttacggat ctggaacatt acgccttcaa
    49981 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    50041 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    50101 gactgatatc gcgactgcga attggcgaac cagccatgat ttcagttggc agcaagcgca
    50161 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaagg
    50221 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    50281 cttcaccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    50341 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    50401 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    50461 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    50521 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    50581 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacac
    50641 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    50701 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    50761 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    50821 gtgccggacc gcccatcagc gggcctggta cctgtagccc ggccaccgtc attgagacat
    50881 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    50941 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    51001 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    51061 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    51121 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    51181 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    51241 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    51301 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    51361 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    51421 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    51481 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    51541 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    51601 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    51661 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    51721 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    51781 gatagcattg ccgtagtttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    51841 cagtggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    51901 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    51961 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    52021 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    52081 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    52141 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgctagcg ggaggaaaca
    52201 gcgccctccc cggcatatta ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg
    52261 atctcaacac gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg
    52321 ccagtgactt tctgtagctc ttccggacgc agtttaattt tgcgtttccc aacatgaatg
    52381 gtcagcgtgc tttgcggcgg cagaacgtac agttgcgcca gaccatcctc gccacgcgcg
    52441 gcttctgccg atggaatgtt gataatcttg ttgcctttgc ccttcgacag ctgcggcaga
    52501 tcacttaccg ggaacatcaa catacggcct gcctgagtga ttgccagcag catatcggaa
    52561 gcatcttcaa tcaccaccgg cggcataaca tgggcatttt ccggtaaggt gatcaaagcc
    52621 ttacccgcac ggttacgcgc taccagatcg ttaaaggtgc agacgaaacc gtaacccgca
    52681 tcggaagcca tcagcagttt ctgatcgtcg ctttccatca gcatatggtc aacggtcgcc
    52741 ccaggcggca acgttaattt gccggtgagt ggttcgccct gaccacgcgc cgacggcagc
    52801 gtaatcgggt cgatggcata gctacgaccg gtggaatcaa caaacactac cggttggtta
    52861 ctcttacctt tcaccgccgc tttgaagcta tcacccgctt tataattcag gcccggcgcg
    52921 tcgatatcat ggcctttagc gctgcgtacc cagcccatct gcgacagcac aatggtgaca
    52981 ggttcagacg gcagcatgtc gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac
    53041 ggcgaacgac gatcgtcacc gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg
    53101 ttattcattt tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc tttttccagc
    53161 tcactctgct caccgcgaat cttcatctct tccagtttgg caagatgacg cagtttcagt
    53221 tcgaggatcg cttccgcctg ggtttccgta aggccaaacc gcgacatcag cgccggtttc
    53281 ggttcatctt cgttacgaat gatctcaatc acttcgtcga tgttgagaaa cgccaccagc
    53341 aaaccttcca ggatatgcag gcgcttgagg actttctcca gacgatagtt cagtcggcgg
    53401 cgcacggtat cgcgacggaa caccagccat tcggagagga tttccagcag gtttttcacc
    53461 gccggacgac catccagacc gatcatattg aggttaatgc gatagctctt ttccagatcg
    53521 gtggtagcga agaggtggtt catcacctga tccatatcca cgcggttgga acgcggcaca
    53581 atcaccagac gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc
    53641 agctttttgt tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga
    53701 tgcggtaatg cgctgataac caccgcgcca tcttctttct tccacaccgc gcgcatacgc
    53761 actgaaccac gtccgttctc gtagatttta cgaatctcgg cgcgcgaagt gataatttcc
    53821 gcttcagtcg gataatccgg cccctgcacg atatccagca gctgatcgag cgtagttttc
    53881 ggctggtcga ttaatgcgat tgccgcctga gctacttcac gcaggttatg cggtggaata
    53941 tcggtcgcca tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg
    54001 gcaggtagca ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc
    54061 ccctgcccca gctcgctcaa tagcagctcg gaatatttcg acaaccggga ttcggtgtaa
    54121 cgcattgccg cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg accatcaacc
    54181 agcggataac ggtaagagaa cggctgcgcc atcaggacca tcgcttcata acaggcgcta
    54241 tcgccgtgcg gatggtattt acccagtacg tcaccgacgg tacgggccga ttttttaaat
    54301 ttggcgctgg cattcaggcc cagttcagac atcgcataca caatgcggcg ctgaacaggt
    54361 ttcagaccat caccaataaa cggcaacgca cggtccatga tcacgtacat ggagtagttt
    54421 aagtaggcgt tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc gctcattaat
    54481 tctgattcct caacttattc gccagccttc aaaggggcaa tattgccgga gatactacat
    54541 tatctgccgc gtcgagtcac aggagatcag cgtgaatagc gccgccggac gtgctccgac
    54601 ggcgataaag gcatcaatgt gccttgatat acaactcttt gctgtagact tgatcggcat
    54661 agagggcagc acaaggttgc gcccatgcca caccactcgg catgggggtc cgctccgggc
    54721 gattcgagaa gttgaggtta tgcgagtcca gggaagccta aagcatcaaa gtagtcgact
    54781 gtaagtatac ggttcagccc agtttagctg tgtctccacc agtgccccgc gcctcctgcc
    54841 ataagtgcgg cttgctcatg gatatccccg agacggctta cggcgcaccg gataataaat
    54901 ttcgcgtata catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt
    54961 gacattgttc gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc
    55021 atcacgccaa cggcacataa acatccgttt ccagtcgctg ctctgtcacc ccttcggcaa
    55081 gattggtgta gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc
    55141 agtggcgaat aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcggg
    55201 ccacggcata acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa
    55261 tcggttcgct aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag
    55321 gggcgtccca ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt
    55381 tacgccacat gataaatttc gccacactgt agttgagcaa ctcagggcta cagcggtgcg
    55441 gcaacatcgc cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg
    55501 tcattttgcc tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg taacaattac
    55561 ggatttactt tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact
    55621 tcaccctgaa tctcaaccgt atctttcggc gtcaccgtca caccgttcca gcgtttgtga
    55681 tcgatatcaa cattgatagt accgctggca tctttaaaca cgtagagatc gtcagagatg
    55741 cgttcaacga tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt
    55801 tctacagtcg ttacgctgcc gttcggcccc tgaaatcctc cggcctgact ttgcgttacc
    55861 gatgggccag aaaaaccgcc ctgctctgct gccatcaccg gtgcactgca cagggccatt
    55921 actgcgatta ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga
    55981 gcatttaaca agatagtcct taacaacttc ttaagggaaa aaaataaaat ttaatgctgt
    56041 acagagcgcg ttacaacacg gtttactggc agcaaatacg gttatcgcag ggatgaaaaa
    56101 atgcgaattt tactgataga agatgacatg ctgattggtg acggcatcaa aactggcctt
    56161 agtaaaatgg gttttagtgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt
    56221 tatagcgcac cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc
    56281 gatattttgc gcgaatggcg agagaaaggt cagcgtgagc cggtactgaa cctgaccgcg
    56341 cgtgatgcgt tagcggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt
    56401 aaaccttttg cgttgataga agtcgccgcc aagctggagg ctctgatgcg ccgaaccaac
    56461 ggccaggcca gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc
    56521 gccacgctgg ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta
    56581 ctgatgcgta acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc
    56641 tgggacgaag aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa
    56701 ctcggtagtg attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga
    56761 aatttaccca acgtcttagt ctgcgcgtca ggctgacgct aatcttttta attctggcct
    56821 cggtgacctg gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg
    56881 aattgttcga cacccaactg atgctgtttg ccaaacggtt aagtacgctc gatctcaacg
    56941 aaatcaacgc ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg
    57001 atgacgatgc gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaatgatg
    57061 gcgataacgg agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac
    57121 tggtcggtga agacgatcct tggcgttttg tctggatgac ctcacctgat ggcaaatatc
    57181 gcatcgttgt tggccaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg
    57241 ggcaattgat cccgtggctg gtcgcactgc cgattatgtt aatcatcatg atggtactac
    57301 tgggtcgtga actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg
    57361 actcggaaaa accactaaac gcgactggcg tacccagcga agtgcggcca ctggttgagt
    57421 cgctaaatca actgttcgcc cgcacacatg cgatgatggt tcgagaacga cgctttacct
    57481 ccgatgcagc tcacgaacta cgtagcccgt taacagcgct gaaagtgcaa accgaagttg
    57541 cgcagctctc tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg
    57601 ggatcgatcg cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac
    57661 tggataacct tcaggacgtc gcggagatcc cgcttgaaga tctcctacaa tcgtcggtga
    57721 tggatattta ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgccc
    57781 acggcatcaa acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg
    57841 ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    57901 cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca
    57961 agctgcatga catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaagg
    58021 taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag attttactaa
    58081 tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca aataacgagc
    58141 gagaatacat atagccaaaa ctcgaacatt tcccgcataa agagttttct taagataaga
    58201 ataataagtg gcgtaagaag aaaaaatgct gcaatcattg cttgcaatat acgatcttcg
    58261 cctgaaacgg aaggtatact gcaaatattg cctcccgact caagcatcca gtcatactta
    58321 ttgaaagcaa aaaaagaatc tattaaccaa taagtaagca ttatatatag caatgcttca
    58381 aatattgttt taattttctg ctttaacata tatcagtacg ctcataaaaa tccatttata
    58441 attatcaaca tgacacgttg atattttcat cttttactta ttcaaagcca tattttcaat
    58501 ccccaaaaaa accgaaccaa acgtgtaaat tattccggtg cgagtaagaa ttatgaggaa
    58561 tggctatcag tattgtcatt ttcagaaaat atttatcctg catcggtgag tcagagctag
    58621 atcagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc acagtaccgc
    58681 tctgtgacgt actatagtcg acaaacgtct caccttgaga ttaaaaaatg agcaacatcc
    58741 tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac gacaccctga
    58801 ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg
    58861 acagcgacta caatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct
    58921 ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatat attgatgatg
    58981 tattcaccga aggtcacggg actctgtatg ccagcgatgg tcgtacccgc aaagatccgt
    59041 cgaaaaaata cggttccggc agcctggtac agggcaaaaa atatatgctt tctctaacct
    59101 ggaacgcacc aatggaagcc ttcaccgaaa aagatcagtt cttccacggc gttggcgttg
    59161 acggtgtgta tctgccgttc cataaagcaa accaattcct cggtatggaa ccgctgccga
    59221 catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc
    59281 gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca
    59341 tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat
    59401 tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg
    59461 cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg
    59521 tgatgattga gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa accccgcaca
    59581 tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatc cgtattctgc
    59641 agccagggat ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc
    59701 tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
    59761 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
    59821 cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca cgcaattgca
    59881 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
    59941 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
    60001 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
    60061 cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt
    60121 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgtcag
    60181 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
    60241 aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc tgatattcac
    60301 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccactagg aacagttcgg
    60361 tacggttaag gccctgcgcg gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg
    60421 tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg
    60481 ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg atgaaggcat
    60541 ctttcaccac gcccgaaacg aatgccgcac actgacgcga agagagacgc tctttggtct
    60601 gcccggcaaa ctgcggatcc tgcattttaa ccgacagaac ataggcgcag cgatcccaga
    60661 tatcttccgc cgacagcttc acgccgcgcg gcagaatgtt gcggtattca cagaactcac
    60721 gcatcgcgtc caacaggccc tgacgcagac cattaacatg ggtaccgccc tgcatcgttg
    60781 ggataaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
    60841 cccagtccac agcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg
    60901 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc
    60961 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtactg
    61021 ctttggcctt cagcacatgc gtcaggcgtg aaacagaaaa acgcgggctg tcaaagaagg
    61081 tttcatccgg ccagaagtgc acgctggtac cggtattgcg tttaccgcaa gtgccgacaa
    61141 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
    61201 ggcgcacgtt aacttctacg cgcttcgaca aggcgttaac taccgaaatc cccacgccat
    61261 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
    61321 aaagaatcag ttcaaccgcc ggtactcctt cttccgggtg aatatccacc ggcatcccgc
    61381 gcccatcgtc aatcacttct aacgactggt cagcatgtaa gattacatcc acgcgttttg
    61441 cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag
    61501 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
    61561 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
    61621 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
    61681 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc
    61741 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
    61801 gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca
    61861 tattgctgcc cggtgtaggg gttctcgttc tgaccgagat agtccgtcag caattcaaac
    61921 gggcgtaccg ccgggtttac caccactgcg ggcagcataa aacattgcga caaccaggtg
    61981 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
    62041 acaatggatt ccagcagttc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
    62101 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
    62161 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
    62221 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
    62281 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
    62341 agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt
    62401 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
    62461 ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac
    62521 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca
    62581 gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca
    62641 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc ttcgccgggg
    62701 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
    62761 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
    62821 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctggtgtg
    62881 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagggctt
    62941 cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa aatccttcct ctggcctcac
    63001 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctagt
    63061 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
    63121 ggttaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg
    63181 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
    63241 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
    63301 tcactagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
    63361 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
    63421 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt
    63481 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
    63541 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
    63601 catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg
    63661 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
    63721 aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac
    63781 gggcaacatc ttccacactt tcaccctctt caatcatccc ggcaaccatc tccagtagcc
    63841 agggggtttc gctggtgtcg tacgcggcaa tccgaatctg ctcaatcagc acaacttcat
    63901 cacgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc
    63961 gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg
    64021 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
    64081 taacgggcag gttgtctggc ttaagcattg ttaatgtcct agcactaata gtgaattaaa
    64141 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tatgttaaat tgaggcacat
    64201 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
    64261 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
    64321 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
    64381 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
    64441 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
    64501 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
    64561 ctcgaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
    64621 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga
    64681 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
    64741 cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac
    64801 ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta
    64861 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
    64921 gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt
    64981 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
    65041 gtcgctgtta caggcacgct tgagccagga cctggagcgc gagcaaattc gccaggcgca
    65101 ggatggtcac ttaccgactc tggatttaac ggcttctacc gggatttctg acacctctta
    65161 tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgatagca atatgggcca
    65221 gaacaaagtt ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
    65281 ggtgaaacag gcacagtaca actttgttgg tgccagtgag caactggaaa gcgcacatcg
    65341 tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcttcta tcagtagtat
    65401 taacgcctac aaacaagccg tagtttccgc gcaaagctca ttagacgcga tggaagcggg
    65461 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcgaccacca cgctgtacaa
    65521 cgccaagcaa gagctggcga atgcgcgtta taactacctg attaatcagc tgaatattaa
    65581 gtcagccctg ggtacgttga acgagcagga tttgctggca ctgaacaatg cgctgagcaa
    65641 accggtttcc actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc
    65701 tgatggttat gcgcctgata gcccggcacc cgtcgttcag caaacatccg cacgcactac
    65761 caccagtaac ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt
    65821 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
    65881 caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa
    65941 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca
    66001 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
    66061 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
    66121 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
    66181 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gtcagcaagc
    66241 accagcccag gctggcatgg caccagaaaa ccaggcgcag gctcagcaat ccagcgggag
    66301 tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg
    66361 atttgcacag cagccgctgt tctcctcgaa aaacccggcc agtccggctt acggtaaata
    66421 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
    66481 gaagacggca atggcaccaa aaccggcgac caccactacc gttacccgtg gcggttttgg
    66541 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc
    66601 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgtgag
    66661 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
    66721 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
    66781 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
    66841 aaattccgca taccaaaaca cacctggagt tttgtgcgtc aatcatggct gactcaccag
    66901 ccatcgcttt attcgcgtct tgatctggcg tgggatggca ctggcgaacc taaacttctg
    66961 gaaaataacg ccgatacgcc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
    67021 ctggaagatc agcttaacgc cggtaacttg tcggagggca gcgaccagtt taacagtctg
    67081 caagaaaaac tgatcgatcg cttcgttgag ctgcgtgaac agtatggctt ccagttgctg
    67141 catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac
    67201 tgcgcaaccg aagctgaaat cgccaccgag ttcctctaca tcgacgagat aggtttaggc
    67261 gaaaaaggcc agttcacgga tttacaggat caggtgattt ctaacctgtt caagctttat
    67321 ccgtgggaat ttatgctgcg cgaaatgttc tccaccaagc tggaagatgc tggcgtacgc
    67381 tggctggaac cggcgtggaa gagcattatc tccaacaagg cacttctacc gctactgtgg
    67441 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
    67501 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
    67561 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
    67621 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
    67681 ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt gatcacccag
    67741 gatatgtctc ggttttatcc acatattttt gttgaataag ccacgatacc ggatggcact
    67801 cgccatccgg taattgttag cctatctgca ccgacagcat acttaaactg cccatttcta
    67861 taccatcgac cggaatggta attggctcct gcccatccca cacacctaac acatacaaca
    67921 acggcagata gtgctctggc gttgggttcg ataacgcgcc accttcatgg tcgagataat
    67981 tcaccagagg atgctgctct accggccctt gccacgtcag attcgctttc acatactcat
    68041 taaacgacgt cgcccacgga tacggtgaac tatcaccgtg ccacttcact gtgcgcaggt
    68101 tatgcaccac gttaccgctg gcgaccaaca ttattccttc atcacgcagc gccgcgagtt
    68161 tgcgccccat ttcgaaatgc caggcgacag gtttgctact gtcgatactc aactgcacca
    68221 tagggatatc ggcgtcagga tacatcttaa tcagcacgcc ccacgagccg tggtcaaagc
    68281 cccaggcttc tttatccagc gtcaccggga tcggcgctaa cagctcaacc agacgctgtg
    68341 ccagcgcagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
    68401 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
    68461 agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc
    68521 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    68581 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    68641 cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    68701 ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc
    68761 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    68821 gacaaaaacc ctcgaaccgc ttactggcgt tttcgctagg ttttgcggcg gggatcatgt
    68881 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgccgg
    68941 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ctttggcctg gaccgcatgt
    69001 tgccgcatgc gcatccgcag gatttaatgc aaaaatcggt gcagctgttg ccaaaatcga
    69061 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    69121 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    69181 tcgcgttgca caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg
    69241 ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg
    69301 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcaatca
    69361 tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    69421 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    69481 gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt
    69541 ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgtc
    69601 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    69661 cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc
    69721 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    69781 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    69841 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    69901 ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa aaaccgaagc
    69961 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    70021 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    70081 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    70141 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    70201 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    70261 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    70321 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    70381 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    70441 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    70501 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    70561 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    70621 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact
    70681 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    70741 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    70801 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    70861 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    70921 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    70981 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    71041 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    71101 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    71161 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    71221 ctcgtgagaa actggcgctg cttgaacaaa gaatcagcga actggaaaat cgcagtactg
    71281 agatcaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta
    71341 atgcactgta aattatgaat taatcctcta atttacagtg ttttctatta cattatcgct
    71401 taatcccatt cagagtatca tcacaaaaat gaatgattgc aattgaaaat ataattgtta
    71461 tgtattcttt aagttttact taatcgatat ttagactggc cc
//
LOCUS       DADLDT020000004        70704 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000004 DADLDT020000000
VERSION     DADLDT020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70704
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.004"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..794
                     /locus_tag="JQH22_000231"
     CDS             <1..794
                     /locus_tag="JQH22_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFB2204687.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="JQH22_000232"
     CDS             805..1896
                     /locus_tag="JQH22_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2204688.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JQH22_000233"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JQH22_000233"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFB2204689.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="JQH22_000234"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="JQH22_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFB2204690.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="JQH22_000235"
     CDS             4858..5253
                     /locus_tag="JQH22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFB2204691.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="JQH22_000236"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="JQH22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFB2204692.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="JQH22_000237"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="JQH22_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HFB2204693.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="JQH22_000238"
     CDS             complement(6562..7602)
                     /locus_tag="JQH22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFB2204694.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="JQH22_000239"
     CDS             complement(7675..8310)
                     /locus_tag="JQH22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFB2204695.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="JQH22_000240"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="JQH22_000240"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFB2204696.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="JQH22_000241"
     CDS             complement(8936..9379)
                     /locus_tag="JQH22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFB2204697.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="JQH22_000242"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="JQH22_000242"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFB2204698.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="JQH22_000243"
     CDS             complement(9719..10222)
                     /locus_tag="JQH22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFB2204699.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="JQH22_000244"
     CDS             complement(10216..10740)
                     /locus_tag="JQH22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFB2204700.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="JQH22_000245"
     CDS             10949..11944
                     /locus_tag="JQH22_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFB2204701.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="JQH22_000246"
     CDS             11953..12831
                     /locus_tag="JQH22_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFB2204702.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="JQH22_000247"
     CDS             12912..13919
                     /locus_tag="JQH22_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFB2204703.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="JQH22_000248"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="JQH22_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFB2204704.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="JQH22_000249"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="JQH22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFB2204705.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="JQH22_000250"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="JQH22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFB2204706.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="JQH22_000251"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="JQH22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFB2204707.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="JQH22_000252"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="JQH22_000252"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFB2204708.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="JQH22_000253"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="JQH22_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFB2204709.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="JQH22_000254"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="JQH22_000254"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFB2204710.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="JQH22_000255"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="JQH22_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFB2204711.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="JQH22_000256"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="JQH22_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFB2204712.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="JQH22_000257"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="JQH22_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HFB2204713.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="JQH22_000258"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="JQH22_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFB2204714.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="JQH22_000259"
     tRNA            complement(27653..27729)
                     /locus_tag="JQH22_000259"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="JQH22_000260"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="JQH22_000260"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFB2204715.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="JQH22_000261"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="JQH22_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="JQH22_000262"
     tRNA            complement(31513..31599)
                     /locus_tag="JQH22_000262"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="JQH22_000263"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="JQH22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFB2204716.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="JQH22_000264"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="JQH22_000264"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFB2204717.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="JQH22_000265"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="JQH22_000265"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFB2204718.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="JQH22_000266"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="JQH22_000266"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFB2204719.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="JQH22_000267"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="JQH22_000267"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFB2204720.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="JQH22_000268"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="JQH22_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFB2204721.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="JQH22_000269"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="JQH22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFB2204722.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="JQH22_000270"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="JQH22_000270"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFB2204723.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="JQH22_000271"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="JQH22_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFB2204724.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="JQH22_000272"
     CDS             complement(41211..42176)
                     /locus_tag="JQH22_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFB2204725.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="JQH22_000273"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="JQH22_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFB2204726.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="JQH22_000274"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="JQH22_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFB2204727.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="JQH22_000275"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="JQH22_000275"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFB2204728.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="JQH22_000276"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="JQH22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFB2204729.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="JQH22_000277"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="JQH22_000277"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFB2204730.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="JQH22_000278"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="JQH22_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFB2204731.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="JQH22_000279"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="JQH22_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFB2204732.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="JQH22_000280"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="JQH22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFB2204733.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="JQH22_000281"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="JQH22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFB2204734.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="JQH22_000282"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="JQH22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFB2204735.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="JQH22_000283"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="JQH22_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFB2204736.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="JQH22_000284"
     CDS             49714..50679
                     /locus_tag="JQH22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFB2204737.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="JQH22_000285"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="JQH22_000285"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFB2204738.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="JQH22_000286"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="JQH22_000286"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFB2204739.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="JQH22_000287"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="JQH22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFB2204740.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="JQH22_000288"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="JQH22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFB2204741.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="JQH22_000289"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="JQH22_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFB2204742.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="JQH22_000290"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="JQH22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFB2204743.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="JQH22_000291"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="JQH22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFB2204744.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="JQH22_000292"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="JQH22_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFB2204745.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="JQH22_000293"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="JQH22_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFB2204746.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="JQH22_000294"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="JQH22_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFB2204747.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="JQH22_000295"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="JQH22_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFB2204748.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="JQH22_000296"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="JQH22_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="JQH22_000297"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="JQH22_000297"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFB2204749.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="JQH22_000298"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="JQH22_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFB2204750.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="JQH22_000299"
     CDS             complement(62540..63469)
                     /locus_tag="JQH22_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFB2204751.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="JQH22_000300"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="JQH22_000300"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFB2204752.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="JQH22_000301"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="JQH22_000301"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFB2204753.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="JQH22_000302"
     CDS             70219..70698
                     /locus_tag="JQH22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HFB2204754.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17315 a        19179 c        17549 g        16661 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aagaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcacggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaag
//
LOCUS       DADLDT020000005        67101 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000005 DADLDT020000000
VERSION     DADLDT020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 67101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67101
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.005"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JQH22_000303"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JQH22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFB2204755.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JQH22_000304"
     CDS             complement(1151..1237)
                     /locus_tag="JQH22_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFB2204756.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JQH22_000305"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JQH22_000305"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFB2204757.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JQH22_000306"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JQH22_000306"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFB2204758.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="JQH22_000307"
     CDS             3586..3630
                     /locus_tag="JQH22_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204759.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JQH22_000308"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JQH22_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFB2204760.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JQH22_000309"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JQH22_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFB2204761.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JQH22_000310"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JQH22_000310"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFB2204762.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JQH22_000311"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JQH22_000311"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFB2204763.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JQH22_000312"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JQH22_000312"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFB2204764.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JQH22_000313"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JQH22_000313"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFB2204765.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JQH22_000314"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JQH22_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFB2204766.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JQH22_000315"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JQH22_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFB2204767.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="JQH22_000316"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="JQH22_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFB2204768.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="JQH22_000317"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="JQH22_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFB2204769.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="JQH22_000318"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="JQH22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFB2204770.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="JQH22_000319"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="JQH22_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFB2204771.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="JQH22_000320"
     CDS             complement(16982..17746)
                     /locus_tag="JQH22_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2204772.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JQH22_000321"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JQH22_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFB2204773.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="JQH22_000322"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="JQH22_000322"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFB2204774.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="JQH22_000323"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="JQH22_000323"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFB2204775.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="JQH22_000324"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="JQH22_000324"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFB2204776.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="JQH22_000325"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="JQH22_000325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFB2204777.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="JQH22_000326"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="JQH22_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFB2204778.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="JQH22_000327"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="JQH22_000327"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFB2204779.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="JQH22_000328"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="JQH22_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFB2204780.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="JQH22_000329"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="JQH22_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFB2204781.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="JQH22_000330"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="JQH22_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFB2204782.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="JQH22_000331"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="JQH22_000331"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFB2204783.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="JQH22_000332"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="JQH22_000332"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFB2204784.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="JQH22_000333"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="JQH22_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFB2204785.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="JQH22_000334"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="JQH22_000334"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFB2204786.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="JQH22_000335"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="JQH22_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFB2204787.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="JQH22_000336"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="JQH22_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFB2204788.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="JQH22_000337"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="JQH22_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFB2204789.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="JQH22_000338"
     CDS             complement(40422..41816)
                     /locus_tag="JQH22_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2204790.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="JQH22_000339"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="JQH22_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFB2204791.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="JQH22_000340"
     CDS             complement(42159..43445)
                     /locus_tag="JQH22_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFB2204792.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="JQH22_000341"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="JQH22_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="JQH22_000342"
     CDS             complement(44452..45222)
                     /locus_tag="JQH22_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFB2204793.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45694..47208
                     /gene="caiT"
                     /locus_tag="JQH22_000343"
     CDS             45694..47208
                     /gene="caiT"
                     /locus_tag="JQH22_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HFB2204794.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47239..48381
                     /gene="caiA"
                     /locus_tag="JQH22_000344"
     CDS             47239..48381
                     /gene="caiA"
                     /locus_tag="JQH22_000344"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFB2204795.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48510..49727
                     /gene="caiB"
                     /locus_tag="JQH22_000345"
     CDS             48510..49727
                     /gene="caiB"
                     /locus_tag="JQH22_000345"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFB2204796.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49801..51354
                     /gene="caiC"
                     /locus_tag="JQH22_000346"
     CDS             49801..51354
                     /gene="caiC"
                     /locus_tag="JQH22_000346"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFB2204797.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51463..52248
                     /gene="caiD"
                     /locus_tag="JQH22_000347"
     CDS             51463..52248
                     /gene="caiD"
                     /locus_tag="JQH22_000347"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFB2204798.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52254..52844
                     /gene="caiE"
                     /locus_tag="JQH22_000348"
     CDS             52254..52844
                     /gene="caiE"
                     /locus_tag="JQH22_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFB2204799.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52930..53325)
                     /gene="caiF"
                     /locus_tag="JQH22_000349"
     CDS             complement(52930..53325)
                     /gene="caiF"
                     /locus_tag="JQH22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFB2204800.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53587..56808)
                     /gene="carB"
                     /locus_tag="JQH22_000350"
     CDS             complement(53587..56808)
                     /gene="carB"
                     /locus_tag="JQH22_000350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFB2204801.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56826..57974)
                     /gene="carA"
                     /locus_tag="JQH22_000351"
     CDS             complement(56826..57974)
                     /gene="carA"
                     /locus_tag="JQH22_000351"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFB2204802.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58430..59251)
                     /gene="dapB"
                     /locus_tag="JQH22_000352"
     CDS             complement(58430..59251)
                     /gene="dapB"
                     /locus_tag="JQH22_000352"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFB2204803.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59418..60332)
                     /gene="rihC"
                     /locus_tag="JQH22_000353"
     CDS             complement(59418..60332)
                     /gene="rihC"
                     /locus_tag="JQH22_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFB2204804.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60398..61348)
                     /gene="ispH"
                     /locus_tag="JQH22_000354"
                     /gene_synonym="lytB"
     CDS             complement(60398..61348)
                     /gene="ispH"
                     /locus_tag="JQH22_000354"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFB2204805.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61350..61799)
                     /gene="fkpB"
                     /locus_tag="JQH22_000355"
     CDS             complement(61350..61799)
                     /gene="fkpB"
                     /locus_tag="JQH22_000355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFB2204806.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61886..62380)
                     /gene="lspA"
                     /locus_tag="JQH22_000356"
     CDS             complement(61886..62380)
                     /gene="lspA"
                     /locus_tag="JQH22_000356"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFB2204807.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62380..65196)
                     /gene="ileS"
                     /locus_tag="JQH22_000357"
     CDS             complement(62380..65196)
                     /gene="ileS"
                     /locus_tag="JQH22_000357"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFB2204808.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65239..66180)
                     /gene="ribF"
                     /locus_tag="JQH22_000358"
     CDS             complement(65239..66180)
                     /gene="ribF"
                     /locus_tag="JQH22_000358"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFB2204809.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66188..66406)
                     /locus_tag="JQH22_000359"
     CDS             complement(66188..66406)
                     /locus_tag="JQH22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFB2204810.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66509..66772
                     /gene="rpsT"
                     /locus_tag="JQH22_000360"
     CDS             66509..66772
                     /gene="rpsT"
                     /locus_tag="JQH22_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFB2204811.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16291 a        18260 c        17267 g        15283 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatatttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaatgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg cctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaaccgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaagaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctagtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagttcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagccacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggaa ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggttgaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acactatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggta caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccag cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata
    45361 ttgttaagtt cctcaccaat attgaaaaca tggcgtagca aaaagaaatt tttaatattg
    45421 ttttatagat caccaatatt gaaagtcaga tgctattttt ttacccacaa aagaatataa
    45481 caaactgatt aataaccaaa attaacgaaa aacgccctct attcactcga aacaaaaatg
    45541 tgacaccaat cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt
    45601 ccacgaggtt aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    45661 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    45721 atagaaccga aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca
    45781 gtcagagatc tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    45841 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    45901 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc
    45961 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    46021 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga
    46081 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    46141 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    46201 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggt
    46261 accatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    46321 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    46381 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    46441 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    46501 ctgggttggg tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    46561 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    46621 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc
    46681 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    46741 ggcatggtga tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac
    46801 actctgctgt tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt
    46861 gtggcgcgcg ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    46921 ggcttcttca tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc
    46981 ctggcgatgt ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt
    47041 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    47101 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    47161 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    47221 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    47281 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    47341 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    47401 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    47461 atggagctgg gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc
    47521 aataccttcc tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgcggc
    47581 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    47641 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    47701 tttattacca gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    47761 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    47821 accaaacttg agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat
    47881 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    47941 gaagagttcg accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc
    48001 gcctttgaag atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt
    48061 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    48121 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    48181 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag
    48241 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg
    48301 cgtgtagacc gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg
    48361 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc
    48421 aggcctacgg gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat
    48481 gcgctacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac
    48541 cgctggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac
    48601 aaatgttcgc agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca
    48661 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    48721 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    48781 tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    48841 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    48901 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    48961 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    49021 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    49081 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    49141 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    49201 cctactacgc cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg
    49261 tgggcattac ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta
    49321 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    49381 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    49441 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    49501 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    49561 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    49621 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    49681 acattcagga gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca
    49741 gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca
    49801 atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac
    49861 ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat
    49921 cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc
    49981 aaaggcaaca aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc
    50041 gggctggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa
    50101 agcgagtgga tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat
    50161 cctatgtatc aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca
    50221 gatgtggcac ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa
    50281 cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc
    50341 acctccggca ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc
    50401 gctggatatt actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta
    50461 atgcctgcgt ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg
    50521 gccacctttg tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag
    50581 taccgcgcca ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg
    50641 ccttcagcga acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg
    50701 gagcaggaaa aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg
    50761 atgacggaaa ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg
    50821 tcgattggtc gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg
    50881 ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc
    50941 aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta
    51001 cataccggcg ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc
    51061 tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc
    51121 gccacccatc cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat
    51181 gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt
    51241 tttcgcttct gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga
    51301 aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa
    51361 cgtcagggaa ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca
    51421 gggatgttgt cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat
    51481 ctgacccgca atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt
    51541 gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa
    51601 ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta
    51661 aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga
    51721 ttaaccgaaa ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt
    51781 ggcggcggtt tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc
    51841 ttcgccctgc cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg
    51901 ccgaagatcc tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc
    51961 gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg
    52021 gataacgccc gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg
    52081 ctgaaagaga tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt
    52141 cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg
    52201 ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct
    52261 attacgcctt tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg
    52321 ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac
    52381 tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca
    52441 ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg
    52501 gagcgatcct gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa
    52561 ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag
    52621 caggctttag tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca
    52681 gtgttagtga cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg
    52741 tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc
    52801 gcccccgttt gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac
    52861 ctggttgctg gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac
    52921 gagataaaat taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt
    52981 aagttccgtg agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc
    53041 ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag
    53101 tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct
    53161 tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat
    53221 atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc
    53281 ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc
    53341 acaaattaaa agtctggcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat
    53401 tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta
    53461 tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct
    53521 catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccacgac
    53581 acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc
    53641 ggcattcagc gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt
    53701 atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt
    53761 ggtgttgatg atgtaggtat attcgccatt cttgatacgg tcctgaatgt gcggacggcc
    53821 ttcatgcacc ttgtttacca gacgcggatt gatacccgct tcgcccagca caatcgccgt
    53881 gccgtgggtc gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac
    53941 gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga
    54001 gttgctgccc agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac
    54061 ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcctggga atttattgaa
    54121 cggcagcacc acttctttca ccgagtagta cggcggaata acttctttgg ttacgccctg
    54181 ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt
    54241 ggctttggag acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac
    54301 ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg cgcacctgca attcgaaggc
    54361 cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg
    54421 cagcgaacat gcggagtcac cggagtgcac acccgcctgc tcgatgtgct ccatgatgcc
    54481 gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc
    54541 aaggaaatga tccagcaaca ctggcgcatc gttagacacg ctaaccgccg tctggaagta
    54601 gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga
    54661 cggacgcacc accagcgggt agccaatctc ttttgccttc tcaactgcca tttcaatagc
    54721 agtaacggtg gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg
    54781 ttcacggtct tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc
    54841 ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc
    54901 tttcggcttc tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta
    54961 gaggcgatcg gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat
    55021 ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc
    55081 gataccctga ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt
    55141 agacggattc gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc
    55201 aaactctgcc gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg
    55261 cagcttacgg atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa
    55321 gcctttgcgt ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc
    55381 cactttctct tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt
    55441 agtcaggttg aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca
    55501 gatacgctct gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc
    55561 atccaggctc actttcgggt cgaagccagt tgcgcccact tccaggccgc gcagcgcttt
    55621 ttgcagggat tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg
    55681 agtggtcaga cggtcgttag caccggcgaa tttttcgaag ttgaagcgag gaattttagt
    55741 aaccacatag tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt
    55801 catcagttcg tctagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa
    55861 accggtcgct ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac
    55921 aatcaggcga ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac
    55981 gccgatttca cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcggt
    56041 cagcgtttgg gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa
    56101 gttttcgata gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc
    56161 gtactctttc cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc
    56221 cagaccgcgg gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct
    56281 accgcccatg gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaacagc
    56341 cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt
    56401 catcgctacg tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc
    56461 aatcatggtg acaccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt
    56521 cagcgccgtc tgaccgccca tggttggcag caccgcgtcc gggcgctctt tttcaataat
    56581 cttgcgtacc acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc
    56641 ggtcatgatg gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag
    56701 ggctttacat gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg
    56761 gcccgcaccc agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact
    56821 cctgattact tagcggtttt acggtactgc tcaattaact tgataaagtg gtcgaacaac
    56881 ggcgcggcgt cgtgtggacc agggctggct tcagggtgac cctggaagct gaacgccggc
    56941 ttatcgatgc gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc
    57001 aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc
    57061 accacgtttt tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt
    57121 ttgacagtct tcgcaccgct cgccagcgcc agcagctgat gaccgagaca gatgccgaat
    57181 accggaatat cggtttcgag gaacttctgg atggcggtaa tggcgtaatc gcacggggcc
    57241 gggtcgccag gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc
    57301 gcagaagttt gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg
    57361 atgttgcgct tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt
    57421 ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct
    57481 gcggtggtca cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt
    57541 tctaacgcca gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct
    57601 ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta
    57661 tggcgcttca ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc
    57721 aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta
    57781 gtgccgacat tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga
    57841 tcagtgagga tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc
    57901 gaacctgttg cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc
    57961 gctgacttaa tcaaaacacc ctccagagaa tactcactca ctttatttgc atattaattc
    58021 acagtgatga cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc
    58081 attctggaga tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt
    58141 ggctcatgca tgttttacag gcattaagcg acaaaaacat catctaacct gttctggaaa
    58201 acgcttgcgc agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat
    58261 ggtcaaaatt tacattaaaa caacaaaaat agaagacgaa acatgtaatt tttggcagtt
    58321 atagaaaatt taaaagcata acaccaaaaa taaaagggct aaaaattaaa gccctttata
    58381 attaataaat taaatgtgtt acttttgcat cataataaac attttgtggt tacaaattat
    58441 tgagatcaag cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag
    58501 ccgatcttac cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca
    58561 gacgctcgcc aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca
    58621 cggtagcaaa accaatggta cctggcacac gttcaccggt atggccttca cgactgtaga
    58681 ccgcgcaatc tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg
    58741 cggtgcctga cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg
    58801 tgtagtcacc catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa
    58861 cgctaaaatt ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt
    58921 taccggcttc gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tgccgacaaa
    58981 aagcgagatg gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat
    59041 cttttatcgc atcaagactg ctttgcacgg taacgcctgt tttccctgca ccagccagct
    59101 caccggcgtc gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg cccagctgca
    59161 cgccttctaa tgccagtgcc gcctgaatca actggcggcc catacgcccc ccggctcccg
    59221 cgatggcaac gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga
    59281 ccgttttcag agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa
    59341 attaatgata aacagggagg gatatcagtg aaaggcatga gtcgatgaat gactgcatgc
    59401 cgataacatg tgacaggtta cggcaccaga gccagcacct cagccaccca ctgttggaag
    59461 ccttttacat tcagatccaa tgccacctgt acattggctg gtttgcccag gcaaccgtcg
    59521 atatcaacca ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc gacaaaacac
    59581 ggcttgaggg tgaataagtc cgggcgcacc agccaggcga tggcgcagag atcgtgcatt
    59641 cgcagaccgc tttgcatact gccgctacgg tagtggctaa acagggcgtg gagcattttt
    59701 ccggtgcgat tcaactccgg taaagtagcg agatagtcag gggttaatat tgcctgattg
    59761 gtgacatcca gaccgcacat gacaatttcg atgccgctac ggaagacaca ggcagccgct
    59821 tctggatcgg cagcgatatt aaactcggca tttggtgtac agttgccgcg accagcagaa
    59881 ccgcccatga tcaccagacg acgaatatac ggtttacatt ccgggcattg taaaagtaac
    59941 agcgcaatat tggttaacgg gccgatggcc accagggtaa cgggctctgg tgcacgcatc
    60001 agggcatccc gaatcgccag aaacgccggt atcccgagtg gctttcggtt gtgctcaaca
    60061 aagtcgtagc cagccattcc cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt
    60121 accagcggca cagcggctcc ctgggcgagc ggaatctccg cattccagaa atgcagcagt
    60181 tgcagggcat tgcgggtagt tttctcaacc gagacattac ccgcgacggt ggtcatcagt
    60241 tgcaggtcga gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg
    60301 gggtcagtat cgaggaagat aggtaaacgc atgttttctt cataaaaaat gccggtaaca
    60361 agaccggcat tttcgcataa cttaggctgc taatgactta atcgacttca cgaatatcga
    60421 cacgcagctc tttcggcact tcgaaaacaa tattttcttc acggccttcc agcggaatgg
    60481 cttcaccacc acccaactgc tgcaaacgtg ccaccacatt ctgcaccaga atatccggag
    60541 ccgatgcgcc cgcagtcacg ccgacgcatt taacctcttt cacccactct tcctggatat
    60601 ctttcgcatc gtcaatcaaa aacgcgtgtt tgcccatacg ctgggccagc tccgccagac
    60661 ggttggagtt ggaggagttt ttcgaaccga ccaccaacac aacttccgcc tgttctgcca
    60721 gggcgcgtac cgcttcctga cggttagtcg tggcgtagca gatgtcatct ttgcgcggac
    60781 cgacaatttt cgggaagcgt ttacgcagcg cgtcgatcac atcagacgtg tcatccaccg
    60841 acagcgtggt ttgggtcata aaggagagct tctcttcgtt tttgaccgtc agtttccaca
    60901 catcgtctgg cgattcgacc agatacattc ccccttcggg gttgctgtac tgccccatcg
    60961 tcccttccac ttccgggtgc ccggcgtggc cgatgagaat agattcttcg ccacggcgac
    61021 tggcgcgggc aacttccata tgcactttgg tcaccagcgg acaggtggca tcaaacaccg
    61081 tcaaatcgcg gcttttcgct tcgttacgaa ccgcctgaga aacaccgtgt gcggagaaaa
    61141 tcaggatcgc gccgtccggc acttcgctaa tctgctcaat aaagatagcc ccacgctcgc
    61201 gcaagctatc gaccacgtag cggttatgca ccacttcgtg acggacatat atcggtgcgc
    61261 cgtaaatggc cagcgcgttt tcaacaatgc tgatagcgcg gtctaccccg gcacaaaaac
    61321 cacgcgggtt ggccaacagg atctgcatgt tacgcctcca gtgccggatc gatttccagc
    61381 acttcaatat caaaatgaac ggtctgcccg gccagcggat ggttgaaatc aacggtaatg
    61441 gagtcgccgt taatttcgcg gatcacacca ggcatctcac tgccatccat tgcggtaaaa
    61501 agcatgattg cgccaatttc tggctcgcct gcatccataa attcacggcg ggagaagtac
    61561 tgaatcaggt ccggtgacgg cacgccaaac gccgcatctg gctccagcga gaaggtggtt
    61621 ttgtcgccca ctttcagccc cagcaggtgt tgctccagcc cttcagaaag agaagcatcg
    61681 cccaggcgga acagcgccgg tttaccgttg ttgcgagtag actcggcggt ggtgccatcg
    61741 tcgagtttta gcgtgaagtg caccaggacg gcgctattgc tctgtacaga ttcagacatg
    61801 caggtttgct cttatttttg aggatttttg ccggatgcgg cctacgattt cgtagcaatc
    61861 tgtaggccgc atccggcagg gtttattatt gttttttcgc tttggaaggt aaaaaccctt
    61921 ccagcacgat cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga
    61981 agtgccagtc gccgacgtag aagtcgatca tatcgacaac gaagccgtgc cacaggcggt
    62041 cgaacaggtt gcccagcgcg ccgccaataa tcagcgcgta agcgatattg tttagcttct
    62101 gcgtggcctt cgagcgatac atcatcactg ccaggagcac gctaatacca atcgcaatac
    62161 cggcaaagaa ccaacgctgc cagccgccgc tatcggcaag gaaactaaac gccgcgccat
    62221 agttacgcgc ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa
    62281 agttctggag gatcaggtat ttgctgccca gatcgataat cagcacgact accaccagcc
    62341 acagccagcg tagccctgtt gaacagatcg attgactcat caggcaaact tacgtttttc
    62401 accgtcaccg gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt
    62461 gccgacatcc tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg ctttactcaa
    62521 cgcgactttc agccctttga gtacttcgct ctgctgagca tcagcaggtg cgtcgttata
    62581 gtctgcaacg gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc ccagcgcggt
    62641 cagtttcgcc gccagttccg gttctgcata caaggttact gccgcttcca gcgagccacc
    62701 cactttcttg tcggcacgcg cttgctcaat gactttgttc acttcgccac gcactttcaa
    62761 cagctcgtcc cagaacgcat cgttcattgc ttcactgtct gccagaccaa acaggccttc
    62821 gtaccactca ccggtgaaga cgtatttttc acgttcgccc ggcagatagc cccacacttc
    62881 atcagcggtg aaggagagga ttggtgccat ccagcgcacc agcgcttcag cgatgtgata
    62941 cagcgcagtc tggcagctac gacgcgccac gctatccgct ttggcggtgt actgacggtc
    63001 tttgatgatg tcgaggtaga aggaacccat ctcaacggag cagaagcgca tcagacgctg
    63061 taccacttcg tggaaatcgt atgcttcgta cgccttgagg atgtcttcct gtgccgcttt
    63121 cgcacaacct acggcccagc gatccagtac caccatctct tccggtttca ccatatcttt
    63181 tgctggatca aaaccattca ggtttgccag caggaagcgc gcggtgttac ggatacgacg
    63241 atagctatcg gcagcacgtt tcaggatctc gtcagaaacg gccatttcac cggtgtagtc
    63301 ggttgatgcc acccacagac gcagaatatc cgcgcccagt ttgttcatca catcctgcgg
    63361 cgaaacggtg ttgccgatgg atttagacat cttgcgaccc tgaccatcca cggtaaagcc
    63421 gtgggtcagt acctgacgat acggcgcttt gcctttcatc gcggtggaga tcattaggga
    63481 agacatgaac caaccacggt gctggtcaga accttccaga tacatgtccg ctgcgtgacc
    63541 ggcaaattcc ggacgcacgt caacaacaga agagtgggta gatccggagt caaaccatac
    63601 gtcgagagta tccggcactt tcacatactg gtcagcttcg tcgccgagga tctctttcgc
    63661 atcgagatcc caccacgcct ggatgccgtc aacttcaacg cgtttagcca cttcttccat
    63721 cagttcgagg gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg
    63781 tacgccccag gtgcgctgac gggagataca ccagtcagga cggttagcaa ccatcgactc
    63841 gatacgcgcc tggccccagt ccgggagcca ctgcacgcct ttgatctctt tcagtgactg
    63901 cgcacgcaga cctttctgat ccatgctgac gaaccactgc ggtgtcgcgc ggaagatgat
    63961 cggcgttttg tgacgccagc agcacggata gctgtgctgc attttctcaa cgtgcagcag
    64021 cgcgcctttt tcctgcagca gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc
    64081 atccagcgtc ggataagtgc ccggcagata agtgccgtcc gggccaaccg ggttagcggt
    64141 ttccaggccg tatttctgac cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg
    64201 aacggcaccg gtaccggcat ccagggtaac gtgatcgccg aggattgccg gaacgtcgaa
    64261 gcccataaac ggatgggtaa agcgcagcag ctcaagctct gcacctttta ccgtgccgag
    64321 aatggtgtaa tcggtcacgc cgatacgctg cattacgctt tcaaccagat ctttcgccag
    64381 aatcacggcc tgaccgtcga tctgcaccag cgcatagtcg aaatcaggtg caatagagat
    64441 tgcgcggttc gcaggcagag tccacggcgt agtggtccag atcaccagcg agattgggcc
    64501 gttaacgttg cttacgccaa atttcgcttt cagcgcatcc tgatcgaccg cctggaaagc
    64561 gacgtcaatg gacggagaag ttttgtcgta atactcaact tccgcttctg ccagtgcaga
    64621 acggcagtct acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac cattgccgat
    64681 gattttgccc agcgcgcgga tgatgttggc ttcagttttg aagtccatag tcagatacgg
    64741 gtgcgaccag tcgcccagca cgcccagacg gataaagtct ttgcgttgac cgtcaacctg
    64801 ggtcgccgcg tattcgcggc acttggcgcg gaactcggcg gcggtgaatt tctcacccgg
    64861 cttaccgtat tcttgctcta ctttcagctc gatcggcaga ccgtggcagt cccagccagg
    64921 cacatacggc gagtcataac cggaaagccc tttggacttc acgataatgt ctttcagaat
    64981 cttgttaacc gagtgaccaa tatgaatgct gccattcgca taaggagggc catcatgcag
    65041 aatgaaggtt tttttgcctt ttttagccgc acggatgatg ccgtacagat catcatcagt
    65101 ccaacgcgcc agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt
    65161 ttccggcaaa ttcagggttg atttatagtc actcatcaga ttctcggttc cgtgtttcgg
    65221 tttgattaca taacaggctt aagccggttt tgttagccca aaaaattcgc gggcggttaa
    65281 ttcatcacgc gcaatctgcg ctttcagttc gtccagcgac gcaaatcgct gctcattgcg
    65341 tattttttta cgcagcacta cttgtatatg gcgaccgtaa aggtccattg caacatctaa
    65401 caaatgcact tccagctgct ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc
    65461 cacgccgggt aacggctttt caccgaggcc cagcacttct accgcataaa cccctttcac
    65521 cggggaaacc tgacggcgta gcggtacatt cgccgtcggg aaacctatag tgcgccctaa
    65581 ttcatcaccg tggactacac gcccggagat ggcaaacggg tgccccagta aactttctgc
    65641 cagagccaga ttgtcatcag ccagcgcctg acgcacggcg gtgctgctga tacgcacacc
    65701 accttcgcaa aaagtttgcg tactggtgat atcgaagccg tattccatgc cagctttctg
    65761 taataacaag aaatcgcctt cacgaccagc gccaaagcgg aaatcatcac ctacggcaag
    65821 aaattttacg cgcaaatgct tcaccaaaag atcgctgatg aaattttgcg cggttaacgc
    65881 cgcaaaacgc ctgtcgaaac gcacgcacag cacgtaatca acgccacact ctgcaaggta
    65941 acgcagtttt tcccgcagcc gggtcagtct tgccggggct ttatcggtag caaacagttc
    66001 cagtggttga ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc gcccttcttc
    66061 ctgcaagccc tgtaacagcg cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt
    66121 cagcacacac ccttcttgcg gggcctggct gagattatgt atgccgcgta tcagcttcat
    66181 gtctggctca aaacagtgaa aatcgtccga gtataccttg tacagcggtc aaggttaacc
    66241 ggcgattgag taccgaaatc agaaagaagt ggcttttcat tgccatggag caaatcacgg
    66301 gaagaaactg accgcctgct gcaattttta tcgcggaaaa gctgtattca caccccgcaa
    66361 gctggtagaa tcctgcgcca tcactacgta acgagtgccg gcacattaac ggcgcttatt
    66421 tgcacaaatc cattgacaaa agaaggctaa aagggcatat tcctcggcct ttgaattgtc
    66481 catatagaac acatttggga gttggacctt ggctaatatc aaatcagcta agaagcgcgc
    66541 cattcagtct gaaaaggctc gtaagcacaa cgcaagccgt cgctctatga tgcgtacttt
    66601 catcaagaaa gtatacgcag ctatcgaagc tggcgacaaa gctgctgcac agaaagcatt
    66661 taacgaaatg caaccgatcg tggaccgtca ggctgctaaa ggtctgatcc acaaaaacaa
    66721 agctgcacgt cataaggcta acctgactgc acagatcaac aaactggctt aatcgccaat
    66781 ttgctgaagc tttgtgaaaa agcccgcgca agcgggtttt tttatgcctg ctgcttttgt
    66841 gatgcgtgag agaacacact gccggttgcc agatagtcaa aagagacatg ccgttgtact
    66901 atctgaactc ttcgtggctt aagaatcgta gaaccatcgg ctgatacatt ctgaacaaga
    66961 atatctactc aatctccaga ctcccccaca aagaatatgg atattgtgat acacattgag
    67021 gtattaaact taagaccact ctatttttga attaaattcc tatatacttt aaccattcat
    67081 aaatatctcc tcattagacc t
//
LOCUS       DADLDT020000006        65613 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000006 DADLDT020000000
VERSION     DADLDT020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.006"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JQH22_000361"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JQH22_000361"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFB2204812.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JQH22_000362"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JQH22_000362"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFB2204813.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JQH22_000363"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JQH22_000363"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFB2204814.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JQH22_000364"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JQH22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFB2204815.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JQH22_000365"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JQH22_000365"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFB2204816.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JQH22_000366"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JQH22_000366"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFB2204817.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JQH22_000367"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JQH22_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFB2204818.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JQH22_000368"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JQH22_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFB2204819.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JQH22_000369"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JQH22_000369"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFB2204820.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JQH22_000370"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JQH22_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFB2204821.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="JQH22_000371"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="JQH22_000371"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFB2204822.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JQH22_000372"
     CDS             complement(15665..16702)
                     /locus_tag="JQH22_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFB2204823.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="JQH22_000373"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="JQH22_000373"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFB2204824.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="JQH22_000374"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="JQH22_000374"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFB2204825.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="JQH22_000375"
     CDS             19444..19884
                     /locus_tag="JQH22_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFB2204826.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="JQH22_000376"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="JQH22_000376"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFB2204827.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="JQH22_000377"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="JQH22_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="JQH22_000378"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="JQH22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFB2204828.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="JQH22_000379"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="JQH22_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFB2204829.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="JQH22_000380"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="JQH22_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFB2204830.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="JQH22_000381"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="JQH22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFB2204831.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="JQH22_000382"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="JQH22_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFB2204832.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="JQH22_000383"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="JQH22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFB2204833.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="JQH22_000384"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="JQH22_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFB2204834.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="JQH22_000385"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="JQH22_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFB2204835.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="JQH22_000386"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="JQH22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFB2204836.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="JQH22_000387"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="JQH22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFB2204837.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="JQH22_000388"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="JQH22_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFB2204838.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="JQH22_000389"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="JQH22_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HFB2204839.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="JQH22_000390"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="JQH22_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFB2204840.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="JQH22_000391"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="JQH22_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFB2204841.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="JQH22_000392"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="JQH22_000392"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFB2204842.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="JQH22_000393"
     CDS             37412..37681
                     /locus_tag="JQH22_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFB2204843.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="JQH22_000394"
     CDS             complement(37684..38043)
                     /locus_tag="JQH22_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFB2204844.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="JQH22_000395"
     CDS             38184..38810
                     /locus_tag="JQH22_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFB2204845.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="JQH22_000396"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="JQH22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFB2204846.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="JQH22_000397"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="JQH22_000397"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFB2204847.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="JQH22_000398"
     CDS             41650..42315
                     /locus_tag="JQH22_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFB2204848.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="JQH22_000399"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="JQH22_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFB2204849.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="JQH22_000400"
     CDS             complement(42949..44166)
                     /locus_tag="JQH22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2204850.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="JQH22_000401"
     CDS             44300..45349
                     /locus_tag="JQH22_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFB2204851.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="JQH22_000402"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="JQH22_000402"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFB2204852.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="JQH22_000403"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="JQH22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HFB2204853.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="JQH22_000404"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="JQH22_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFB2204854.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="JQH22_000405"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="JQH22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFB2204855.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="JQH22_000406"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="JQH22_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFB2204856.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="JQH22_000407"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="JQH22_000407"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFB2204857.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="JQH22_000408"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="JQH22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFB2204858.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="JQH22_000409"
     CDS             complement(51544..51903)
                     /locus_tag="JQH22_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFB2204859.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="JQH22_000410"
     CDS             complement(51900..52175)
                     /locus_tag="JQH22_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFB2204860.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="JQH22_000411"
     CDS             complement(52234..53358)
                     /locus_tag="JQH22_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2204861.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="JQH22_000412"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="JQH22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFB2204862.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="JQH22_000413"
     CDS             complement(56090..57157)
                     /locus_tag="JQH22_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2204863.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="JQH22_000414"
     CDS             complement(57523..59145)
                     /locus_tag="JQH22_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFB2204864.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="JQH22_000415"
     CDS             complement(59407..60996)
                     /locus_tag="JQH22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFB2204865.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="JQH22_000416"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="JQH22_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="JQH22_000417"
     CDS             complement(62499..62975)
                     /locus_tag="JQH22_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204866.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="JQH22_000418"
     CDS             complement(62968..63732)
                     /locus_tag="JQH22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFB2204867.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="JQH22_000419"
     CDS             63978..64739
                     /locus_tag="JQH22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFB2204868.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="JQH22_000420"
     CDS             complement(64846..65346)
                     /locus_tag="JQH22_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204869.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15938 a        17620 c        16160 g        15895 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccaa gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gctagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcc
//
LOCUS       DADLDT020000007        60413 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000007 DADLDT020000000
VERSION     DADLDT020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.007"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..316
                     /locus_tag="JQH22_000421"
     CDS             <1..316
                     /locus_tag="JQH22_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2204870.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="JQH22_000422"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="JQH22_000422"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFB2204871.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="JQH22_000423"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="JQH22_000423"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFB2204872.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="JQH22_000424"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="JQH22_000424"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFB2204873.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="JQH22_000425"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="JQH22_000425"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFB2204874.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="JQH22_000426"
     CDS             complement(3720..4949)
                     /locus_tag="JQH22_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFB2204875.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="JQH22_000427"
     CDS             complement(5013..5453)
                     /locus_tag="JQH22_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFB2204876.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="JQH22_000428"
     CDS             complement(5558..6328)
                     /locus_tag="JQH22_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2204877.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="JQH22_000429"
     CDS             complement(6325..7251)
                     /locus_tag="JQH22_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2204878.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="JQH22_000430"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="JQH22_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFB2204879.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="JQH22_000431"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="JQH22_000431"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFB2204880.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="JQH22_000432"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="JQH22_000432"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFB2204881.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="JQH22_000433"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="JQH22_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFB2204882.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="JQH22_000434"
     CDS             12958..13305
                     /locus_tag="JQH22_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFB2204883.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="JQH22_000435"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="JQH22_000435"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFB2204884.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="JQH22_000436"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="JQH22_000436"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFB2204885.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="JQH22_000437"
     CDS             complement(15125..15487)
                     /locus_tag="JQH22_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HFB2204886.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="JQH22_000438"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="JQH22_000438"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFB2204887.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="JQH22_000439"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="JQH22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="JQH22_000440"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="JQH22_000440"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFB2204888.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="JQH22_000441"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="JQH22_000441"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFB2204889.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="JQH22_000442"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="JQH22_000442"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFB2204890.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="JQH22_000443"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="JQH22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFB2204891.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="JQH22_000444"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="JQH22_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFB2204892.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="JQH22_000445"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="JQH22_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFB2204893.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="JQH22_000446"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="JQH22_000446"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFB2204894.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="JQH22_000447"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="JQH22_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFB2204895.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="JQH22_000448"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="JQH22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFB2204896.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="JQH22_000449"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="JQH22_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="JQH22_000450"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="JQH22_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFB2204897.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="JQH22_000451"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="JQH22_000451"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFB2204898.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="JQH22_000452"
     CDS             complement(36342..36380)
                     /locus_tag="JQH22_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204899.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="JQH22_000453"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="JQH22_000453"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFB2204900.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="JQH22_000454"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="JQH22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFB2204901.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="JQH22_000455"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="JQH22_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFB2204902.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="JQH22_000456"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="JQH22_000456"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFB2204903.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="JQH22_000457"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="JQH22_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFB2204904.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="JQH22_000458"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="JQH22_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HFB2204905.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="JQH22_000459"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="JQH22_000459"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFB2204906.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="JQH22_000460"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="JQH22_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFB2204907.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="JQH22_000461"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="JQH22_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFB2204908.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="JQH22_000462"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="JQH22_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFB2204909.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="JQH22_000463"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="JQH22_000463"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFB2204910.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="JQH22_000464"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="JQH22_000464"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFB2204911.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="JQH22_000465"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="JQH22_000465"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFB2204912.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="JQH22_000466"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="JQH22_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFB2204913.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="JQH22_000467"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="JQH22_000467"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFB2204914.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="JQH22_000468"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="JQH22_000468"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFB2204915.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="JQH22_000469"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="JQH22_000469"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFB2204916.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="JQH22_000470"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="JQH22_000470"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFB2204917.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="JQH22_000471"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="JQH22_000471"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFB2204918.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="JQH22_000472"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="JQH22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFB2204919.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="JQH22_000473"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="JQH22_000473"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFB2204920.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="JQH22_000474"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="JQH22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFB2204921.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16733 c        14846 g        14362 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgaacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacttcctt gatgtaaata aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact ggacgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga attgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcaataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacagctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgac tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DADLDT020000008        60283 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000008 DADLDT020000000
VERSION     DADLDT020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 60283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60283
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.008"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..756)
                     /locus_tag="JQH22_000475"
     CDS             complement(<1..756)
                     /locus_tag="JQH22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HFB2204922.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKES"
     gene            complement(853..1329)
                     /gene="greB"
                     /locus_tag="JQH22_000476"
     CDS             complement(853..1329)
                     /gene="greB"
                     /locus_tag="JQH22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFB2204923.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            1557..2276
                     /gene="ompR"
                     /locus_tag="JQH22_000477"
     CDS             1557..2276
                     /gene="ompR"
                     /locus_tag="JQH22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFB2204924.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            2273..3625
                     /gene="envZ"
                     /locus_tag="JQH22_000478"
     CDS             2273..3625
                     /gene="envZ"
                     /locus_tag="JQH22_000478"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFB2204925.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(3701..5323)
                     /gene="pckA"
                     /locus_tag="JQH22_000479"
     CDS             complement(3701..5323)
                     /gene="pckA"
                     /locus_tag="JQH22_000479"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFB2204926.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            5702..7426
                     /locus_tag="JQH22_000480"
     CDS             5702..7426
                     /locus_tag="JQH22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFB2204927.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(7570..8439)
                     /gene="hslO"
                     /locus_tag="JQH22_000481"
     CDS             complement(7570..8439)
                     /gene="hslO"
                     /locus_tag="JQH22_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFB2204928.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(8464..8865)
                     /gene="hslR"
                     /locus_tag="JQH22_000482"
     CDS             complement(8464..8865)
                     /gene="hslR"
                     /locus_tag="JQH22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFB2204929.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(8876..9544)
                     /gene="yrfG"
                     /locus_tag="JQH22_000483"
     CDS             complement(8876..9544)
                     /gene="yrfG"
                     /locus_tag="JQH22_000483"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFB2204930.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(9609..11744)
                     /gene="igaA"
                     /locus_tag="JQH22_000484"
     CDS             complement(9609..11744)
                     /gene="igaA"
                     /locus_tag="JQH22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFB2204931.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            12064..12624
                     /gene="nudE"
                     /locus_tag="JQH22_000485"
     CDS             12064..12624
                     /gene="nudE"
                     /locus_tag="JQH22_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFB2204932.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(12789..15341)
                     /gene="mrcA"
                     /locus_tag="JQH22_000486"
     CDS             complement(12789..15341)
                     /gene="mrcA"
                     /locus_tag="JQH22_000486"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFB2204933.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            15461..16240
                     /gene="hofM"
                     /locus_tag="JQH22_000487"
     CDS             15461..16240
                     /gene="hofM"
                     /locus_tag="JQH22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFB2204934.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            16240..16779
                     /gene="hofN"
                     /locus_tag="JQH22_000488"
     CDS             16240..16779
                     /gene="hofN"
                     /locus_tag="JQH22_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFB2204935.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            16763..17203
                     /gene="hofO"
                     /locus_tag="JQH22_000489"
     CDS             16763..17203
                     /gene="hofO"
                     /locus_tag="JQH22_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFB2204936.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            17193..17597
                     /gene="hofP"
                     /locus_tag="JQH22_000490"
     CDS             17193..17597
                     /gene="hofP"
                     /locus_tag="JQH22_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFB2204937.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            17509..18747
                     /gene="hofQ"
                     /locus_tag="JQH22_000491"
     CDS             17509..18747
                     /gene="hofQ"
                     /locus_tag="JQH22_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFB2204938.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            19148..19669
                     /gene="aroK"
                     /locus_tag="JQH22_000492"
     CDS             19148..19669
                     /gene="aroK"
                     /locus_tag="JQH22_000492"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFB2204939.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            19726..20814
                     /gene="aroB"
                     /locus_tag="JQH22_000493"
     CDS             19726..20814
                     /gene="aroB"
                     /locus_tag="JQH22_000493"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFB2204940.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            20906..22192
                     /gene="damX"
                     /locus_tag="JQH22_000494"
     CDS             20906..22192
                     /gene="damX"
                     /locus_tag="JQH22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFB2204941.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            22299..23135
                     /gene="dam"
                     /locus_tag="JQH22_000495"
     CDS             22299..23135
                     /gene="dam"
                     /locus_tag="JQH22_000495"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFB2204942.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            23153..23830
                     /gene="rpe"
                     /locus_tag="JQH22_000496"
     CDS             23153..23830
                     /gene="rpe"
                     /locus_tag="JQH22_000496"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFB2204943.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            23823..24581
                     /gene="gph"
                     /locus_tag="JQH22_000497"
     CDS             23823..24581
                     /gene="gph"
                     /locus_tag="JQH22_000497"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFB2204944.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            24574..25578
                     /gene="trpS"
                     /locus_tag="JQH22_000498"
     CDS             24574..25578
                     /gene="trpS"
                     /locus_tag="JQH22_000498"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFB2204945.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            25848..26753
                     /locus_tag="JQH22_000499"
     CDS             25848..26753
                     /locus_tag="JQH22_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2204946.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            26770..27132
                     /locus_tag="JQH22_000500"
     CDS             26770..27132
                     /locus_tag="JQH22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFB2204947.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            27216..28379
                     /locus_tag="JQH22_000501"
     CDS             27216..28379
                     /locus_tag="JQH22_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFB2204948.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            28380..29606
                     /locus_tag="JQH22_000502"
     CDS             28380..29606
                     /locus_tag="JQH22_000502"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFB2204949.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            29603..30481
                     /locus_tag="JQH22_000503"
     CDS             29603..30481
                     /locus_tag="JQH22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFB2204950.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            30492..30845
                     /locus_tag="JQH22_000504"
     CDS             30492..30845
                     /locus_tag="JQH22_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFB2204951.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            30857..32161
                     /locus_tag="JQH22_000505"
     CDS             30857..32161
                     /locus_tag="JQH22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFB2204952.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            32173..33258
                     /locus_tag="JQH22_000506"
     CDS             32173..33258
                     /locus_tag="JQH22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFB2204953.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(33418..34149)
                     /gene="phoB"
                     /locus_tag="JQH22_000507"
     CDS             complement(33418..34149)
                     /gene="phoB"
                     /locus_tag="JQH22_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HFB2204954.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34249..35034)
                     /gene="frlD"
                     /locus_tag="JQH22_000508"
     CDS             complement(34249..35034)
                     /gene="frlD"
                     /locus_tag="JQH22_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFB2204955.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(35031..35861)
                     /gene="frlC"
                     /locus_tag="JQH22_000509"
     CDS             complement(35031..35861)
                     /gene="frlC"
                     /locus_tag="JQH22_000509"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFB2204956.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(35911..36933)
                     /gene="frlB"
                     /locus_tag="JQH22_000510"
     CDS             complement(35911..36933)
                     /gene="frlB"
                     /locus_tag="JQH22_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFB2204957.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(36954..38342)
                     /gene="frlA"
                     /locus_tag="JQH22_000511"
                     /pseudo
     CDS             complement(36954..38342)
                     /gene="frlA"
                     /locus_tag="JQH22_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(38586..38753)
                     /locus_tag="JQH22_000512"
     CDS             complement(38586..38753)
                     /locus_tag="JQH22_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFB2204958.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(39000..40373)
                     /gene="cobA"
                     /locus_tag="JQH22_000513"
     CDS             complement(39000..40373)
                     /gene="cobA"
                     /locus_tag="JQH22_000513"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFB2204959.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(40392..41198)
                     /gene="nirC"
                     /locus_tag="JQH22_000514"
     CDS             complement(40392..41198)
                     /gene="nirC"
                     /locus_tag="JQH22_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFB2204960.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(41324..41650)
                     /gene="nirD"
                     /locus_tag="JQH22_000515"
     CDS             complement(41324..41650)
                     /gene="nirD"
                     /locus_tag="JQH22_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFB2204961.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(41647..44190)
                     /gene="nirB"
                     /locus_tag="JQH22_000516"
     CDS             complement(41647..44190)
                     /gene="nirB"
                     /locus_tag="JQH22_000516"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFB2204962.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(44452..45633)
                     /gene="tsgA"
                     /locus_tag="JQH22_000517"
     CDS             complement(44452..45633)
                     /gene="tsgA"
                     /locus_tag="JQH22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFB2204963.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            45904..46485
                     /gene="ppiA"
                     /locus_tag="JQH22_000518"
                     /gene_synonym="rotA"
     CDS             45904..46485
                     /gene="ppiA"
                     /locus_tag="JQH22_000518"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFB2204964.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            46590..46757
                     /locus_tag="JQH22_000519"
     CDS             46590..46757
                     /locus_tag="JQH22_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFB2204965.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            46747..47349
                     /locus_tag="JQH22_000520"
     CDS             46747..47349
                     /locus_tag="JQH22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFB2204966.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            47381..47944
                     /gene="pabA"
                     /locus_tag="JQH22_000521"
     CDS             47381..47944
                     /gene="pabA"
                     /locus_tag="JQH22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFB2204967.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            48030..49250
                     /gene="argD/dapC"
                     /locus_tag="JQH22_000522"
     CDS             48030..49250
                     /gene="argD/dapC"
                     /locus_tag="JQH22_000522"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFB2204968.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(49317..51407)
                     /locus_tag="JQH22_000523"
     CDS             complement(49317..51407)
                     /locus_tag="JQH22_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFB2204969.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(51458..52090)
                     /gene="crp"
                     /locus_tag="JQH22_000524"
     CDS             complement(51458..52090)
                     /gene="crp"
                     /locus_tag="JQH22_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFB2204970.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            52392..52796
                     /locus_tag="JQH22_000525"
     CDS             52392..52796
                     /locus_tag="JQH22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFB2204971.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(52851..53720)
                     /locus_tag="JQH22_000526"
     CDS             complement(52851..53720)
                     /locus_tag="JQH22_000526"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFB2204972.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(53774..53992)
                     /locus_tag="JQH22_000527"
     CDS             complement(53774..53992)
                     /locus_tag="JQH22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFB2204973.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(53986..55008)
                     /locus_tag="JQH22_000528"
     CDS             complement(53986..55008)
                     /locus_tag="JQH22_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFB2204974.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(55008..56921)
                     /locus_tag="JQH22_000529"
     CDS             complement(55008..56921)
                     /locus_tag="JQH22_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2204975.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            57052..57603
                     /gene="kefG"
                     /locus_tag="JQH22_000530"
     CDS             57052..57603
                     /gene="kefG"
                     /locus_tag="JQH22_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFB2204976.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            57603..59340
                     /gene="kefB"
                     /locus_tag="JQH22_000531"
                     /pseudo
     CDS             57603..59340
                     /gene="kefB"
                     /locus_tag="JQH22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            59350..59550
                     /locus_tag="JQH22_000532"
     CDS             59350..59550
                     /locus_tag="JQH22_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HFB2204977.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            59645..60235
                     /gene="slyD"
                     /locus_tag="JQH22_000533"
     CDS             59645..60235
                     /gene="slyD"
                     /locus_tag="JQH22_000533"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFB2204978.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
BASE COUNT        14016 a        15266 c        16488 g        14513 t
ORIGIN      
        1 gctctctttg ggcggctcat cgaactgcgg atcggcattc agcgaaagct ggagtacgcc
       61 ttcgttacgc ccacggaaca tcgccagcgc gcggtgagaa ggcaccgtgg acaacggttc
      121 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
      181 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
      241 ttcggcaaac cgttccatca ggatatagcg cgcgccatcc agcgcggctt tggtatctgc
      301 cacgccttta tcggcatcaa catattgtgc agcggcgact tctggcgtgt gtgacggatc
      361 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
      421 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
      481 gttgatggcc ttcgccagat catcggtgag tttgccttgc tcggaaatgg atttgaggat
      541 cgcctgacgt ctctcttcca gctcgcgcag atagctcaga cgcgtttcca gattacgcag
      601 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgcgataa acggcacggt
      661 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
      721 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
      781 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
      841 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagt tcgcttcccc
      901 ggcaggggta ttcaccaccg ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
      961 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
     1021 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaatacttt
     1081 gccttcctgc tgaggggagt aatcgacgat tttgagattt tccaggcatt tagtgagata
     1141 gcgcacgcga cggtcgattt cacgcagacg ctttttatta tactgatagt cagcattttc
     1201 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
     1261 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
     1321 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
     1381 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
     1441 aatatgcttt gtaacaattt aggctgaaat ttataccaga tttagctggt gacgaacgtg
     1501 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
     1561 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
     1621 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
     1681 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
     1741 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
     1801 tgacggcgaa aggggaagaa gtggaccgta tcgtaggcct ggagattggc gctgacgact
     1861 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tattcgtgcg gtgctgcgtc
     1921 gtcaggcgaa cgaactgcca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
     1981 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agatgagccg atgccgctca
     2041 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
     2101 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
     2161 acgtgcagat ttcgcgtctg cgccgcatgg tagaagaaga tccagcgcat ccgcgttaca
     2221 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
     2281 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
     2341 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
     2401 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
     2461 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
     2521 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
     2581 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
     2641 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
     2701 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
     2761 tacgctggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
     2821 ccgaccgttg gtcgatctcg aacacgcagc cttgcaggtt ggtaaaggga ttattccgcc
     2881 gccgctgcgt gaatatggcg cttcggaggt gcgttccgtt acccgtgcct ttaaccatat
     2941 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     3001 cgacttgcgc acgccgctga cgcgtattcg cctggcgact gagatgatga gcgagcagga
     3061 tggctatctg gcagaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     3121 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atcttaatgc
     3181 agtgctcggt gaggtgattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     3241 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     3301 tatggtggtc aacgccgccc gttatggcaa tggctggatc aaagtcagca gcggaacgga
     3361 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     3421 taagcacctg ttccagccgt ttgtccgcgg cgacagtgcg cgcaccatta gcggcacggg
     3481 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     3541 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     3601 gcagggcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
     3661 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
     3721 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg ataaacagtt
     3781 tcgccagggt ttcggctttt tcctgccact gttccggaga agcgtaggtg ttacgcggat
     3841 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
     3901 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
     3961 tatctttaat cgagatacgt ttgccagtgc cgttccagcc agtgttaacc agataagcct
     4021 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
     4081 acaggaacgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
     4141 cagtaccggc cagtttggcg gtgaagccag agaggaagtg atactgggtt tgatcggcag
     4201 tcaggcgaga aaccggcggc aacacgccga aagcatcagc agtcaggaag ataaccttag
     4261 tcgcgtggcc cgctttggaa accggcttaa cgatgttatc gatgtgatag attggataag
     4321 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
     4381 cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca ggttccgctt
     4441 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaaattaaac acgccgtcat
     4501 cgtcccagcc gtgttcgtca tcgccaatca ggcggcgttt cgggtcggtg gaaagggtgg
     4561 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ccaacgttgg
     4621 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
     4681 tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg cgctcggtca
     4741 ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc gggttagtgc
     4801 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct tcatcgctcg
     4861 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
     4921 cggaaagacg agtatccggg ttcgcaccac agaaagcgtc gacaacgaac agacgtttgc
     4981 cggaaagctg cctggtcacc aggcctttca gatgctgcca ggtttccgga gagagaggtt
     5041 tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
     5101 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
     5161 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
     5221 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
     5281 aagcctcgag ttcttgcggg ggcaaaccat tgttaacgcg catttcactg ctccttagcc
     5341 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
     5401 gatttacgta tctggagaaa ttcatatttg acaaaaaact ccgtgattcc tgtcacgaaa
     5461 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
     5521 tcttggttta tatggcggga atattgtgag tggaatcgca ttcctgaatg aaattgatgc
     5581 aaattcaacc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
     5641 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
     5701 catggataac gttgaacttt caccggcaac acgctggggg atgattgcta ccggattact
     5761 tcaggggctg gtttgctacc tgcttatcgc ctggcttgcc ggaaaaaatc acagttggat
     5821 tgtttatggc gtgcctgcga ccgtcgcgtt ttcatccgtt ttactcttct ccgtgatctc
     5881 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgacagggat
     5941 gagcggttgg ctgaagtggc aaactgatgg catgaacccc tggagagctg aaaaggcgat
     6001 ttgggatttt ggctgctatc tgctgttgat ggcaatgttg ttgctaccgt ggatacaaca
     6061 aagccttcgt atccgcaatg gcagtagccg ctatagctac ttttaccagt cagtatggca
     6121 taacgtactt atattactgg tgatttttct ctccaatggc ctgacgtggc tggtgctttt
     6181 actgtggggt gagttgttta aacttgttgg catcacgttt ttcaatacgc tcttttttgc
     6241 aaccgactgg tttatgtatc ttacatttgg tctggttacc gcactggcgg tgatcctcgc
     6301 gcgtacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgctaa tcgccacggg
     6361 gttgctgccg ttagtatcat tgctaacccc gatgtttatc atcaccctgc cgtttacggg
     6421 cctgagcgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
     6481 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
     6541 gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg tgttcgtcgc
     6601 cacctggggg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
     6661 cgcgctggcg gagctggtgt tactggtgtg gtcgctgggg tattttgtca gtatcgtctg
     6721 gcgtaatggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
     6781 ggtgttggtg atactggtgc ttcttaattc gccggtgctg gacagtatgc gtattagcgt
     6841 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
     6901 catgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
     6961 cgggtttatg aaagacccga aacgcgcgcg ggatctgctg atggcgttag atggagagca
     7021 acatcttcag gaacaggtat cggaaaaagt attcgccgaa aatgtgttaa ttgcccctgg
     7081 ttctgttaaa ccggatgcga cattctggtc ggccttaatc caggatcgct ataacgtgat
     7141 gacctgtatt gaaaaagacg cctgcgtact ggtcgagcaa gatctgaata gtgatggtca
     7201 ggcggagcgg atcctgtttg cttttaatga tgacagagtc attgtctatg gctttgactc
     7261 agacagaaaa gaatgggacg cgcttgatat gagtttactt ccgaacgaaa taacgaaaga
     7321 aaaattactc acagctgcga aggatggaaa actggggacg aggccgaaag cgtggcgcga
     7381 tctgacggta gatggtgaaa cgctggaaat aaacttaagt aagtgacaac gcttttatcg
     7441 gatacgacgc tgtatctgac caaaataatt tgcgatttat ttcataggcg ctggtaagcg
     7501 cagcacatca ggccattaag tcgtaaaatc cggcagagcc ctctctgccg gacatactca
     7561 ttaatgaact tacggatctg ccggagacgc gttgttgcgg atttcagcaa tatccatcgc
     7621 attgaacaga tagtggttac cgcagtaatc acaatgcatg tcaatttcgc catcttccgc
     7681 caggatgcta tcaacttctt catcaggcag cgttttcagc gcatcggcgc aacgttcacg
     7741 cgagcaggtg catttgaact ccacatcctg cggatcgtaa accgtcacct cttcttcgtg
     7801 atacaaacgc cacaacactt cgtttgccgg taaggtcagc agttcttcgg ttttgatggt
     7861 ttcggttagc gtcgccaggt ggtcaaagtc gtcctgttgg gtattttgcg caggcattac
     7921 ctgcaacaac ataccgcctg cagccggttt gccgtctacg tcgccggtgc gaataaacag
     7981 gcgcgtcggc agctgttcag aacgcataaa gtaatcttct aggcaggccg ccagggtatc
     8041 accttccaga ccaactacgc cctgatagcg ttcgccttcg ctcggggtaa tggtgatcac
     8101 cacgtaacca ttgccgacca gcgttttcag gtcggcattt tctggaattt cgccctgcac
     8161 gcgcgccaca ccgcgcattt gctggttatt gttaccgtta ataaccgcca gattcatcgg
     8221 accgtcgccc tgcagctgta cggtgatatc accatcaaac ttcagcgtag cggttaacag
     8281 gctggtcgca accagcagtt ctgccagcac gtttttaacg ggctgcggat aatcgtggtt
     8341 ctcaaggatc tgttgcaggg tttccgaaac ggttaccagt tcgccgcgaa cggcaaaatt
     8401 ttcaaacaga tagcgatgta attggtcatg ttgcggcata atcatctctc ttgcaggtga
     8461 cagttattca ctgtcgccgt gtttaaatcg taacaggtcg cggcgctctt ttttgtccgg
     8521 gcgtcggtcc gggtgcggca tggttaaggc attaagttta cgtgccatcg ccattttttc
     8581 gcgtttctct acactttccg cagtctcttc atacagcaag gctgcctcgc tggcggggcg
     8641 acgctgttca gtaatcgcct ttacaatcac cgtgcgttcg tcatttccct ggcgcagagt
     8701 gagcgtggca ttcagctcga cgattttgct cggcttgctg cgctgcccgt tgtaatgcac
     8761 cttaccgcct tcaatcattt cacgggccag cgcgcgggtt ttataaaaac gggcagccca
     8821 tagccattta tccagtcgaa cctcaacagc aggtttctct ttcatggcgt ctccttcaca
     8881 ttagcgaggg gatcaggcgg cggtagtcat tcagtgacgg atggcgttga tactgtttct
     8941 cggcaatccc ggaatcagga ttagtcacgc cgaggcagta acgaatacca aattgcgcgg
     9001 cagcatcgag aatcgcttcg ctgtcatcaa taaacagcgt tctttcagct ttcagacccg
     9061 tagcttcggc caccgcatgc cataaccgct gatcctcttt cggataacca aatgtgtggg
     9121 tggaaagtaa taaatcaagg tgtgcgtcca gacccgtatg ctcaagtttt accgccaggt
     9181 tgtgcggatg agcattggtg agcaaaattc gctgcttacc gctggctttc agtgcctcaa
     9241 gaaacggaat ggtatcttca cgcagtacgg cgcgcggtcc catctcggtg gtcatcgcac
     9301 agatatccag acccagttgc tcactccagt aatcaagaca gtaccagttt agcgtatgct
     9361 gtacgtcgtg atattgctgg cgcatatatt ccatcgcttc ctgtggcgta accccgtttt
     9421 tcgcgcccca tgtttcaggc accagctttt gccagaaata gttatcgaag gcgaggtcga
     9481 gcaacgtgcc gtccatatcc agcagaacgg tatctacgtc ctgccaggca atgttgatat
     9541 gcatgaggga aatctccaga gtgaagcaat ttgcgcgaca gggtagcata acctgccgcg
     9601 caaacgtgtt attcgataag gctttctgaa ggggtgatca gttgcgggtt caggcagctt
     9661 tcataatagg cctgaatctc catcatgcga gtgcgatgac gctggtaacg acgccaggcc
     9721 tgtacgccat tgtaaatggc gctacccagc atcgtcagta gcagcaatgt ggtgctgaga
     9781 tagcgccaca ggccggaacg atccgggatc ggatgaaggc caatatgctg cgtaccattg
     9841 gcgtcagtga agatttttgt gacgatacct tcggcgttaa acggcgtatg catcagcatt
     9901 tgtgccagtt tctggaaagc gttccactgt tcttgcggcg ggtagtcgta aagtgatgcc
     9961 gaaggccagg gctgatcaac aaaatcgctg ccttcgtcgc tgacaatcag gaatccgccg
    10021 ggggccggac tgtttagtga ttgtgccgct cgcgccgttt catgcgtgat aaacggcgcg
    10081 gtggaggttg ccaccaggtt atccagcgat tccgcactca ccgggcgtaa taacacattc
    10141 acgccatcga gcttcccggc gttggcgcgt tttaccagcg cgtcccagtc tttactgttg
    10201 ccgagattga ccagcgcatt tttcagtcgc acacagtcat ctttggcaga acataaatcc
    10261 gctgtcttca gtacaatgtc gccaaaatca tcaagcaata ccatgccgga tttttgaatc
    10321 gctgaacgca atgaggcact gacgcgagat tcatcttccg gtttagggtg cagctggcga
    10381 ttaactgctt cagtcaatgc cgtcgctttg ttgaccagtt cagattctgg taatggtaat
    10441 gagcgggcgt cattccagat gatctgcgag cagtcaaacg gtaaaaaagg tgaattggtt
    10501 ttcgcgctcc aggttccgga agtgcgaata ttacacattc ccgtaccgct aatacgcaat
    10561 gtatcgccta cccgcacgcc agcgtcagcc agttgtttta cgctggtggc ttcaatggtc
    10621 tgcgcgcctt tcatccatga gagagtgaat ttcagcggca tatccagcgg gatccagaat
    10681 aacagcataa acagcaccag cagcgagccc gcagcgataa tcgtactgcg cagccagtgc
    10741 tgtaacggaa agttttttac ttcatcatgc agcgaaagat atcgcccctg acgcactacg
    10801 tggcggtcga gatagatatc aatatcggtt tgttgaccga gatcctgagc aatgtatggc
    10861 tgccagtgtg cgggatagac caggtcgata ataccgagtg aaatattgtt gatctgttcc
    10921 tgatcgtttt cgccaaacaa tccccaacga cggggtgtac cgcgcagaca atgaatttct
    10981 cgcagggagg attttgccgg gggcgcgaat agcccccaca gacctgcgcc cagcagcagt
    11041 aatgcgccgc ctgccagcca cggaacaaat acatccgggg taatcaggca gaaaaagaac
    11101 atcaggaagg aggcgacgat cagcaacgct tcacgcagcc cgcgcggacg actcagcgca
    11161 tattcttcat gcgtttcttt gcgaatattg agtagctcga tttgctcact ctcttccccg
    11221 cgaatcgacg cctgcgtgga cggaacaggt tgcaaggcat agccgcgcgt ttcctgcatg
    11281 tactcctgca gcgtatgacc gttgagtgaa ataaccaacg gcagcgaatc ggtatgaatc
    11341 agttcaacgg tattctcatc gttgatgtac tgttcccaga aggggggcag gtggacttct
    11401 accgaatcga ggtagtaacg ccatttattc ggatcgtcgg tagagatgcc gtaacgtgtg
    11461 atagcgtgtg tcagcatcat gacgttgttg ctttcggcat tcagcgccag agagatcggt
    11521 gccgcgctag ctcccgttgg gccaggcacc tgtaatacct gcgtcaggct ctcaagataa
    11581 ttttcaacgg cgctacgttc ttcaggtgtg agtttacgcg tttgcgcatc cgcgaaggcg
    11641 ttggtccagg gcagctgacg ccgtctggat cgcactttta tcagccatcc cgcaagtagt
    11701 gagcaggcca gcaaagcagc taaaaaaatc acaatggtgc tcatgctttc cccatcttac
    11761 ttagtctgtc aggcgtggtc agtcgcaccg gacacaatac atgattctgc ggttggctat
    11821 cggcaagcgt ggagcttaac ttgagcatca ttaagcacat cacagtaacc gctgatcata
    11881 gcaaaccctc tgaaggcgga atatcagcaa attcctggag ttatttcaac ctattgactt
    11941 ttaatttgat ttgaggacga ttatttatta agttacataa atgtaaataa catgcaattc
    12001 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
    12061 acgatgaaca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccgttcc
    12121 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
    12181 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggatgatcac
    12241 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
    12301 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
    12361 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
    12421 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
    12481 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
    12541 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
    12601 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgcttatcag
    12661 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg ataacgcgtt
    12721 cacccgcatc cggcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    12781 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    12841 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    12901 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggaggcggc
    12961 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    13021 tgggcactct tggcaccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    13081 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    13141 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    13201 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cggtaccctg ccagcctggt
    13261 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    13321 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    13381 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    13441 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcactgg aatatcgcat
    13501 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    13561 ttgctgataa accacgggtc cactaggaag ccgccgttcg ccatgaccgc gtagccgcgc
    13621 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    13681 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatcgcc
    13741 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    13801 ggaccagcat actgcggtgg tgagttcttc ggctgccagt cagaaccggc acctgcatcc
    13861 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    13921 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    13981 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accattttgc
    14041 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    14101 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    14161 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    14221 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctc cctgaggatt ggcgctggtg
    14281 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
    14341 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    14401 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgcgcggc ctgctgcact
    14461 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    14521 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    14581 gcgtgataat tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ctgggtgata
    14641 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    14701 gagtagagcg gattaaaggt ggaaggcgct ttcggcaacc cggctatcac cgccatttcg
    14761 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    14821 taggcgcggt aaccaaggta aatcttgttc agataaagct cgaggatttc gtctttcgtc
    14881 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat catcgtgcgt
    14941 tctggactga ggaagaaatt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    15001 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaactccg
    15061 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggtggg
    15121 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    15181 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt caccacatcc
    15241 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    15301 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    15361 ttcatttggg cagtttataa acaaacgcgc ggtagtataa aggcaagcca gacgcattga
    15421 tatacccgtc agagtgacgg gtgataagga gatcatcaca atgacattta agatctggca
    15481 aattggtttg catttacaac agcaagaagc ggcagcggtt gcgatcgtgc ggggcgcaaa
    15541 agaatgcttt ttgcaacgct ggtggcggtt gccgctggcg cacgacatta tcaaagatgg
    15601 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccgtggagtc gcgaactgcc
    15661 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    15721 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    15781 ccgcgagctg gatatggatc cggactccct gcgcttcgat tacagcgaag actcactcag
    15841 ccccgcttat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttacgctggc
    15901 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    15961 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    16021 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    16081 gctggcggca gcgttatccg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    16141 tgacccctgg gaggccgttt ctgttcgtca gccgccgcta ccgccgcctg gtggagactt
    16201 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    16261 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    16321 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    16381 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    16441 acgaagccac gtttgctgga gcaacagcag ttacgcgaac agcgttcgca acggcagcgc
    16501 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    16561 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    16621 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    16681 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    16741 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    16801 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    16861 atcatcgaca caccatgaag agcgcgacgc attaattcga ctacgggcaa gtcatcacca
    16921 gcagtgggcc gcactgtatc gcctggtaga caccactccc ttcagcgagg aaaaaacgct
    16981 gcccttttcg ccactggatt ttcagttatc cggcgcgcaa ctggtttcct ggcatccatc
    17041 cgcgcaggga ggcgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcatttac
    17101 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    17161 tcttttgttc acgctacaac tggagatgcc gcatgagggt taaacgctgg ttgttggcag
    17221 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaatcgccg gaagatctat
    17281 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
    17341 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    17401 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
    17461 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    17521 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    17581 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    17641 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    17701 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    17761 gaaggcaaca ttctctcagt gcattccatt gcctggcaga atgacaatat cgcccgccag
    17821 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    17881 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    17941 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    18001 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    18061 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    18121 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    18181 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    18241 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    18301 ctgctggcct cacatcttca gcctgccagc attaaacagg agagcgaaat tccatatcag
    18361 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggatg
    18421 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    18481 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    18541 gagatcgaaa cgcaggtcga ggtaaaaagc ggagaaacgt tggcgctggg cggcattttt
    18601 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    18661 gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt ggtgtttatc
    18721 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    18781 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    18841 gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    18901 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    18961 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    19021 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    19081 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    19141 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    19201 cactattggg cgccagttag ctcaacagct caatatggaa ttttacgatt ccgatcaaga
    19261 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    19321 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    19381 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    19441 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    19501 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    19561 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    19621 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    19681 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    19741 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    19801 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccag cgaaaccctg
    19861 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    19921 agcgttatcc tccctgacgg cgagcagtat aaaagcctag ctgtactcga taccgtcttt
    19981 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    20041 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    20101 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    20161 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    20221 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    20281 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    20341 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    20401 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    20461 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    20521 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    20581 agttctgccg aaacgcagcg tattataacc ctgctcacgc gggctgggtt accggtcaat
    20641 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    20701 cttgcgggag agatgcgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    20761 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
    20821 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
    20881 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    20941 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    21001 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    21061 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    21121 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    21181 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    21241 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga ccacttccag
    21301 cgatcaaacc gcgtctggcg agaagagtat tgatcttgct ggcaatgcga ccgatcaggc
    21361 gaatggcgtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    21421 gctgccaccg atctcttcta cgccgactca agggcaaacc ccggtggcaa cggatggtca
    21481 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac aaaatcagca
    21541 acagctgaac aatgtggcgg tcaattccac gttgccgact gaacccgcaa cggttgcgcc
    21601 tgttcgcaat ggcaatgcat cgcgtgactc ggcgaaaacg caaaccgctg aacgtccgtc
    21661 cactacgcgc ccagttcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    21721 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
    21781 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    21841 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    21901 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    21961 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    22021 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    22081 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    22141 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    22201 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    22261 ttctccacag ccggagaagg tgtaattagt taatcggcat gaagaaaaat cgcgcttttt
    22321 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    22381 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    22441 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    22501 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    22561 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    22621 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    22681 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    22741 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    22801 gcatggagcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    22861 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    22921 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    22981 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    23041 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    23101 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    23161 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    23221 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    23281 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactacg gcattaccgc
    23341 ccctatcgac gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    23401 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    23461 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    23521 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ccgtcaaccc
    23581 tggtttcggc ggtcagtctt tcattcctca aacactggat aaactgcgcg aagtacgtcg
    23641 ccgtatcgac gagtctggct ttgacattcg tctggaagtg gacggtggcg tgaaggtgaa
    23701 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    23761 cgaccagcca gactataaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    23821 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tcttgatggt acgctggtcg
    23881 acagtgctcc tggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttgcccg
    23941 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    24001 gcgcattgac ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg gggaaaccgc
    24061 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    24121 gctactatgg cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    24181 tgggcgcgtt gcaggctaaa ggcctgccgc taggcttggt caccaacaaa ccgacgccgt
    24241 tcgtcgcgcc gctgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    24301 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    24361 tgggaattgc cccacaacag atgctgtttg tcggcgactc acggaatgat attcaggcgg
    24421 caaaagcggc aggttgccca tcagttggct taacctacgg atacaactac ggcgaggcta
    24481 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    24541 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    24601 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    24661 aacatgcagg atgactacca ttgcgtttac tgtatcgttg accaacacgc gatcaccgtg
    24721 cgccaggatg cacagaagct gcgtaaagcg acgttggata cgctggcctt gtatctggct
    24781 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    24841 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagtcg catgacgcag
    24901 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tggctatccg
    24961 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    25021 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    25081 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    25141 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    25201 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    25261 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    25321 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    25381 tatggtcatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    25441 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    25501 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    25561 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    25621 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    25681 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcttttcg
    25741 caacaacccg aaaaaatgtg aagcgcctcg ccgtttccac atctcgtcgt cgccacttat
    25801 cttcatttta atgaagatat aaatattcat tttattgaaa atatattatg cgtcgaacgt
    25861 ttatcaaaaa agaaggcgtc gtcatcacga cactggcccg ttatttgttg ggtgaaaaat
    25921 gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca tcggttggcc
    25981 tgacgcaggc cgcgttgaaa acgctggaat caagcggagc gatacggatt gaacgccgtg
    26041 ggcgcaatgg cagttatctt gtcgagatgg ataacaaagc attgctgagt catgtggata
    26101 tcaacaacgt ggtatgtgcg atgcctttgc cctatacccg cttgtacgaa ggtttagcga
    26161 gcggattgaa agcccagttt gatggcattc ctttttacta tgcgcatatg cgcggcgcgg
    26221 atattcgcgt ggagtgttta cttaacggtg tctatgacat ggcagtagtt tcccgcctgg
    26281 cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata
    26341 cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag
    26401 taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt tttggcgata
    26461 gtgatgtgga acgagtcgat ctctcttatc acgagagttt acaacgcatt gttaaaggcg
    26521 atgtcgatgc ggtgatctgg aacgtggtgg cggaaaacga actgaccatg ctgggattag
    26581 aggcgacgcc gctaacagac gatccgcgat ttttacaggc caccgaagcc gtggtcctga
    26641 cgcgagtcga tgattaccca atgcaacaat tgctgcgtgc tgttgtagat aaacacgccc
    26701 tgcttgccca tcaacaacgg gtggtgagtg gggaacagga acccagttat taaacgaaag
    26761 gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat tgataaggac
    26821 gtctgcaaag gcatgatgca ggtcgtcaac gtactggaaa aagagtgcca tctgccggtg
    26881 cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat gcgcagtcgc
    26941 cgtggtgaag aaatagagcc gctggataac gagttgctgg cggaactggc gcaatccagc
    27001 cactggcaag ccgttgtgca attgcatcag gagttgttga aggaattcgc tctggaagtt
    27061 aacccgtgtg aagaaggcta ttttctggcc aatctttatg gattatggat ggctgctaac
    27121 gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc aaaaaaatga
    27181 atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc
    27241 agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaaa atcgccccgg
    27301 acagctgggt gatcgacgtg gatcagatac tggaaaacgg taagcggctc attgagacgg
    27361 cgcggcttta cggcattgaa ctgtatctga tgaccaagca atttggtcgt aatccgtggc
    27421 tggcggaaaa attgctggca ttaggctaca gcggcattgt ggcggtggat tacaaagagg
    27481 cgcgagtcat gcgccgcgct ggtttgccag tggcgcatca ggggcatctg gtacaaatcc
    27541 cttgtcatca ggttgctgac gccgttgaac agggcaccga catcatcacc gtgtttactc
    27601 tcgacaaagc gcgggaagtt tctgcggcag cggttaaggc cggacgggtc cagcctgtgc
    27661 tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc ggttttgtac
    27721 agcattcgct gcacgaggtg attgctgaaa ttaagaaact gccggggctg catttagccg
    27781 gactaaccca tttcccttgc ctgctttggg atgaggctgc tgggaaagtt ttgccaacac
    27841 cgaatcttca cacgctggta caagcacggg atcaactggc gaaatctggt attgcgattg
    27901 agcaactgaa cgtaccttca gcgaccagct gcacttcgct gccattgctg gcggaatacg
    27961 gtgcgactca tgccgaaccc ggtcatgcgc tgaccggtac tattccggca aaccagcagg
    28021 gcgatcagcc tgaacgtatc gcgatgctct ggttaagtga aatctcccat catttccgtg
    28081 gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa catgcgctgg
    28141 tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg gatgacagca
    28201 gtatcgacta caccctgccg ctggcaggcg agtatccggt gagcagtgca gtggtgctct
    28261 gttttcgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc ggtattcagc
    28321 gcggcgaacc agaaatagtt ggtcgttatg acagcctcgg caaccctctg gaggcataaa
    28381 tggcgcgatt tgtggtgtta gtgattgata gctttggcgt aggggcaatg aaagatgtca
    28441 cgctggtgcg cccgcaagat gccggggcga atacctgtgg tcacatcctg agccagttgc
    28501 cgcatttgca gctaccaacg ctggagaagc tggggctaat caacacattg ggttatgcgc
    28561 caggcgatat gcagccgtca gattccgcaa cctggggcgt ggcagagctg caacatgaag
    28621 gtggcgatac ctttatgggg catcaggaaa ttttaggcac gcgcccgtta ccgccgctgc
    28681 ggatgccttt tcgcgatgtg attgaccgtg ttgagcaggc attagtttcc gctggctggc
    28741 aggtggagcg ccgtggcgat gatctgcaat ttctgtcggt caatcaggcg gttgcgattg
    28801 gcgataatct cgaggcggat ttaggccaag tctataacat taccgccaat ctctctgtga
    28861 tctcttttga cgacgcaatc aaaattggtc gtatcgtgcg tgaccaggta caggtcggtc
    28921 gggtcattac atttggcggc ctgttaacca acagtcagcg tattctcgat gccgcagaaa
    28981 gcaaagaagg gcgctttatt ggtattaatg cgccgcgttc tggcgcttat gacaacggct
    29041 tccaggtcgt gcatatgggc tacggtgttg atgaaaaagt gcaggtgccg caaaaactgt
    29101 atgaagcggg cgtgccaacc gtgctggtgg gtaaggtggc agatatcgtc agcaatcctt
    29161 atggtgtgag ctggcaaaac ctggtggata gccagcggat tatggatatc accctcgacg
    29221 aatttaacac ccatccgacg gcgtttattt gcatcaatat ccaggaaacc gacctcgctg
    29281 gtcatgcaga agacgtcgca cgttatgccg aacgtttgca ggtcgttgac cgtaaccttg
    29341 cccggcttgt tgaggcgatg cagccagatg attgcctggt cgtgatggcg gatcacggta
    29401 acgatccgac cattggtcac agccaccata cccgtgaagt ggtgccagtg ctggtttatc
    29461 agcaagggct ggtcgccacg cagctcggtg tgcgcaccac gctttctgat gtgggagcta
    29521 ccgtgtgtga atttttccgt gcgccaccgc cacaaaatgg tcgctctttt ctttcctccc
    29581 tccggtttgc aggagacacc ctatgagttt tgatccgacg ggttacaccc tggcccatga
    29641 gcatctgcat attgatctct ccggctttaa aaacaacgtg gactgccgcc ttgatcagta
    29701 tgcgttcatt tgccaggaga tgaacgacct gatgacccgg ggtgtacgta atgtgattga
    29761 gatgaccaac cgttacatgg ggcgcaatgc gcaatttatg ctcgatgtaa tgcgcgaaac
    29821 cgggatcaac gtggtggcct gtactggtta ttaccaggat gcgtttttcc cggaacatgt
    29881 ggtgacccgc agcgtgcagg aactggcgca ggagatggtc gatgaaattg aacagggtat
    29941 cgatggcacg gagctgaaag ccgggatcat cgcggagatc ggctccagcg aaggaaagat
    30001 tacgccgctg gaagagaagg tatttattgc cgctgcgctg gcgcataacc agaccggacg
    30061 cccgatctcc acgcatacgt cgttcagcac catggggctg gaacaactgg ctctgttgca
    30121 agcccacgga gttgatcttt cgcgcgtcac cgttggtcac tgcgatctga aagacaacct
    30181 cgacaacatt ttgaagatga tcgatctcgg cgcgtacgtg cagttcgaca ccattggtaa
    30241 gaacagttac tacccggacg aaaagcgcat tgcgatgctc cacgcgctac gtgaccgtgg
    30301 gttgctgaac cgcgtcatgc tgtcgatgga tattacgcgc cgctcccatt taaaagccaa
    30361 tggtggttat ggctatgact atttattgac cacctttatt ccgcaattgc gccagtcagg
    30421 attcagtcag gccgatgtgg atgtgatgtt acgtgaaaat ccctctcaat ttttccaata
    30481 aggacagact catgaaaaag attggcgttg caggcttaca gcgtgagcag attaaaaaaa
    30541 ccattgaagc gacggctcct ggctgttttg aagttttcat tcacaacgac atggaagcgg
    30601 caatgaaggt gaaatccggg caactagatt attacattgg cgcgtgtaat accggtgcgg
    30661 gcgcggcatt gtcgattgcc atcgcggtga ttggctataa caaaagttgc accattgcca
    30721 aaccaggcat taaagcgaaa gacgagcata tcgccaaaat gatcgctgaa gggaaagtgg
    30781 cgtttggcct ttccgttgag cacgtcgaac acgcgattcc gatgttgatt aaccatctga
    30841 aataaaaggc acgactatgg atctgtatat tcagattatc gtggtggcgt gcctgacggg
    30901 tatgacatcg cttctggcgc atcgctcggc ggctgttttt catgacggca tccacccgat
    30961 cctgccgcaa ctgattgaag gctatatgaa ccgtcgcgag gcggggagta tcgcttttgg
    31021 tctgagcatt ggttttgtgg cctcggtggg gatctctttt accctgaaaa ccgggctgct
    31081 caacgcatgg ttactctttc ttcctaccga tatcctcggc gtactggcga taaacagcct
    31141 gatggcgttt ggtcttggcg ctatctgggg cgtgttgatc cttacttgcc tgttgccagt
    31201 taaccagctg ctgaccgcgc tgccggtgga tgtattaggt agcctcgggg aattaagctc
    31261 gccggtggtt tctgcttttg cactgttccc gctggtggcg attttctacc agtttggctg
    31321 gaagcaaagt ctggtcgccg ccgtggttgt tctgatgacc cgtgtggtag tcgtgcgcta
    31381 tttcccacat cttaaccctg aatccatcga aatctttatt ggcatggtga tgctgctggg
    31441 gatcgcgata actcacgacc tgcgtcatcg tgatgaaaat gacatcgatg ccagcgggct
    31501 ttcggtgttt gaagagcgca cgtcgcggat tatcaaaaac ttaccttata tcgccatcgt
    31561 gggagcattg attgccgccg ttgccagtat gaagattttt gctggtagcg aagtttcgat
    31621 cttcacactg gagaaagcgt attccgcagg cgtgacgccg gaacaatcgc aaacgctgat
    31681 taatcaggcg gctctggcag aatttatgcg cggactgggg tttgtgccgt tgattgccac
    31741 caccgcctta gcaacgggtg tgtatgcagt tgcgggcttt acctttgttt atgcggtggg
    31801 ctatctctcg ccgaatccga tggttgcagc ggtattaggc gcagtggtta tttcggcgga
    31861 agtcttgctg cttcgttcga tcggcaaatg gctgggacgc tacccgtcgg tgcgtaatgc
    31921 gtcggataac atccgtaacg ccatgaatat gctgatggaa gtggcgctgc tggtcggttc
    31981 gattttcgca gcaattaaga tggcgggtta taccggattc tctatcgcgg ttgccattta
    32041 cttcctcaac gaatccctgg gccgtccggt acagaaaatg gcggcaccgg tcgtggcagt
    32101 aatgatcacc ggtattctgc tgaatgttct ttactggctt ggcctgttcg ttccggctta
    32161 aggaaggcac ctatgaagac gtttcctttg caaagcctga ctcttgctga agcgcagcaa
    32221 aagcagtttg cgctggtgga cactatttgc cgccattttc caggcagcga gtttcttgct
    32281 ggcggtgatt tagggttaac tccaagtctg aatcaaccgc gtattaccca acgggtggag
    32341 caggtgctgg ctgatgcatt tcacgcacag gccgcggcgc tggtgcaggg cgcggggact
    32401 ggcgcgattc gcgccgggct ggcggctttg ctcaaaccgg ggcagcgtct tctggtgcat
    32461 gacgcgcctg tttacccgac gacacgggtt attattgagc agatggggct gacgcttatt
    32521 actgctgatt tcaatgacct gtcggcactg aagcaggtcg tcgacgagca acaacctgat
    32581 gccgcgctgg tgcagcatac gcgccagcag ccgcaggaca gctatgtgct ggcagatgtg
    32641 ctggcaacgt tgcgcgcggc aggtgttcca gcgttaaccg atgataacta tgcggtgatg
    32701 aaggtagcgc gaatcggctg tgaatgcggc gcgaatgtct cgacattctc ctgcttcaag
    32761 ctgtttgggc cggggggcgt tggtgcagtg gttggcgatg ccgatgttat tagtcggatt
    32821 cgcgccacgc tttactccgg cggtagccag atccagggct cacaggcgct ggaagtcttg
    32881 cgtggtctgg tgttcgcgcc agtgatgcat gcggtgcagg cgggagtatc cgaacggttg
    32941 ctggctttgc ttaacggcgg cgcggtggcg gaagtgaaaa gcgccgtcat tgccaatgcg
    33001 cagtcgaagg tgttgattgt cgagtttcat cagctgattg ccgccagggt gctggaagaa
    33061 gcgcaaaagc gtggcgcgtt gccttacccg gtcggtgcag agtcgaaata tgaaatcccg
    33121 ccgctcttct atcgactttc aggaacgttt cgccaggcga atccacaact cgaacattgt
    33181 gcgattcgca ttaacccgaa tcgcagcggt gaagagacgg tgctgcggat tttgcgtgag
    33241 agtattgcga gtatttaatg ctggatagca ccgttcggca atcaagctaa attccgcact
    33301 gaacggtccc ctcgcccctt tggggagagg gttagggtga ggggaacgtg cgggatgtaa
    33361 aattcagtgc gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta
    33421 atgtctttta ttatcaatcg ttaaggtgat gcgattggcg cggcagaaga gttcggagac
    33481 gtgcaccggt ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc
    33541 cagttcgcat ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt
    33601 tttgtcgctg accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc
    33661 ttcgacgtaa atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat
    33721 aaacagcggc tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc
    33781 ggggatgttc agcttttcgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt
    33841 ctctttcgtc gacttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag
    33901 ggcgttttca actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac
    33961 gccgtctgcc actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg
    34021 tgtacaaagc tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat
    34081 atcatccagc agccgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt
    34141 agctgacata atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc
    34201 ggcaagtaac agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc
    34261 gtggtactga atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt
    34321 catccccgca gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt
    34381 gtcgataacc gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat
    34441 gctgccgttt tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt
    34501 catcttcaga cgcagcgctt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg
    34561 cggcaccagt gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag
    34621 tttgcccgca gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg
    34681 cacaatgtca tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat
    34741 cacgccttcg gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg
    34801 tgcggtaacg ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc
    34861 ctgcttcagc tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat
    34921 gccgtagcga gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt
    34981 cagttgcggg tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca
    35041 tcctccggca gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg
    35101 ttcatataca tcgtcaccag ctccaccgta cagtaaccct catagccccg atcaataata
    35161 tcgcgcatca gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg
    35221 gccccgtcgc tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa
    35281 ctcatcaccg gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc
    35341 ggcgaaggca ccagcgccag cgcatgaagc acatcattag cattacacac gacgttcgat
    35401 tcatacggcg ttaacggttc gaggatcaga tccatgccga tgttttccgc gtactcacac
    35461 agttcgctta agttctctgc cagccgcccc cagataacgt taggcggcgt gagatagccc
    35521 gcgtgggccg cagaaatcag cgtataaccc gcgttcatct cttttgccat atctatcgcc
    35581 agcttgatca tgtccaggct ttcacgacgc atatgttcat cgcccagcat catgttatac
    35641 ggatagccgt tggtttctgg cgtgtaaccg ataatcggca tctgatacgt ctgcgccagc
    35701 gccttgattt gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg
    35761 ccccaaattt cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata
    35821 ggcagccgct ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa
    35881 atccggcccg ttgccgggcc gggagaggga ttaatattcc accagaccac cgtaatagcg
    35941 gcgttcatcc gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg
    36001 cacgaacatc aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc
    36061 gatgacgatc acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg
    36121 gcgactttca tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag
    36181 cgggccatgg cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat
    36241 cagcgttaca atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata
    36301 aatcatcggc cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg
    36361 taccagatga ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc
    36421 cgcgttcggc gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg
    36481 aatttcccag atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg
    36541 gctgtccgcg cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag
    36601 cgctttgatt acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc
    36661 gtcgaggcga tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag
    36721 atcggaaaag cgatccgcca gatgtttcgc cgtttgcgcc gcgttgagtg gcgagccgca
    36781 ggcaacgaaa taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac
    36841 cagcggaacg tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag
    36901 aaagtccacg gtgcttttat caatatccaa cattttattt tcttcagata acattacgac
    36961 aaagcgttga gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca
    37021 ataacgatga cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc
    37081 agaatgaggc ttgacgcaat ggcgagggtg gtcatcagcc cgaaagccgg agtacgccac
    37141 agcggtttgt aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt
    37201 ttgaaacaca tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg
    37261 aagaagatcc ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga
    37321 tgcacatggc cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc
    37381 ggctggtaca tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca
    37441 acaaagagcc cagcggtgct gccgagtgcg gggatctagg tcagggcgtc ggaaatcggc
    37501 gtttcagaat tggcgagttt gtcgaagggc atcaggccgg aaatcaccag cgccagcagg
    37561 gtgtagagca ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg
    37621 gggtttttaa tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg
    37681 gtggcagaga tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg
    37741 gtaagggcgg caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg
    37801 aacgggataa ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag
    37861 cgcaggtgta gcaacataaa ggcgataatt aatccggcgg cgataaattt accgagcaac
    37921 ggatcgatag gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat
    37981 gacggcgcat cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt
    38041 ccggcatttt tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc
    38101 gcatagacgc acatttgcgg gatcacaatt aacccgccaa tgacaaacgc gagcaccgta
    38161 agccacggcg tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg
    38221 acggttgtcc cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttct
    38281 tggcttccca tagcgatacc tttttcttgt ccaatacgtt gtaggggaga gtttgcagtc
    38341 atttaaaagg taacatgaca atgcatgatg aatataacaa catattatgt tatgtaatgt
    38401 ggaggaagat cacaaaattt cgcacaggat cgcgctgtgg ctaatggatg tagttataaa
    38461 attgaattta aagtgaaaat atttttacgg gcgggggcaa gaaggacata gaaacaaata
    38521 cgccctcgga aaatccagag ggcgtcgggc aattaaaccg gtgttagccg atttctgtca
    38581 gagacttact gtgcagtagg accgcaaccg ccaatgattt tagaaataga aattgccggg
    38641 tgcagcaggt agtcgtaaga gcagttctta tcacggtttt caatatgacc agtacatgca
    38701 gttaacgtag ccagtactgc acctaccagt gcaactttaa taaatttgtt catttacatt
    38761 ccctatgtat tatatattaa ttaatattca ttgattatat gaatataagc agactgcttg
    38821 ttaagaacga tacaggaata tttaattggc gtcagtgagc gaatgactta attttgtaag
    38881 tcgctgtaac ggtgaatata acccattagc agataatatt tattaagggg ttatgtttta
    38941 tttgctgaat atataaaaag agtcattaag aaggccagaa ttctctggcc cgggttaaat
    39001 taatgattgg agaaccagtt cagtttatcg cgcaggccaa caacccgacc aataataatt
    39061 agcgatggac tgttcatttg ctgagcaagt tcaccgagtt gcgtgagcgt accgtcaatc
    39121 acgcgctgcg tgactgccgt tccgttttcg acaattgcca ccggcatttc gccaggcata
    39181 ccgtgttcaa tcagcttttg ctgaatagtc gcggcctgat tcaaccccat atagaacacc
    39241 agcgtctgtt tttctgccgc caggttttcc cagtccagct cgccaccggt ttttaagtgt
    39301 ccggtaatta agcgtacgct ctgggcataa tcgcgatgcg tgagtggaat acccgaatag
    39361 gcagagcaac cagaagctgc ggtaataccc ggaaccaccg agaacggaat acccgcgttg
    39421 cacagtgttt ccagctcttc gccaccacgg ccaaaaataa acggatcgcc acctttcagc
    39481 cgcaccacgc gtttgccttt ttgcgcttcc cgcagcagga tctggttaat ctcttcctgc
    39541 ggtacacagt ggtatcccgc gcgtttgccg acgaaaacgc gatcagcatc gcggcgtacc
    39601 agattcataa tatcgtcaga aaccagacgg tcgtagacca ccacatctgc ctgctgaatt
    39661 tgttgcagtc ctttcagcgt cagcagcccg gcatcgcccg gacctgcacc aaccagcacc
    39721 acttcaccgc gatggtcgag cggttcgttg attaactgtt cggtcgtttc agtaatggct
    39781 ttctgatcgt tgtttgccag cgactgcgcc aggcggtcgt taacgaacaa tttctcccag
    39841 aaacggcgac gctcactcat cgtggcgaac tgttgtttca ctcgcccgcg taattgcccg
    39901 gcatatttcg ccacctggcc cagatgcaac ggcagcagtg attcgagttt ttcgcgcaac
    39961 aggcgcgcca gaaccggaga ggtgccgcca gaggagaccg ctaccattag cggtgagcgg
    40021 tcaataatcg acggcataat aaagctggcg gctttcggcg catcaactac gttacagaag
    40081 atgcgacgag cttcagcggc ttcgctgacg cgctggttaa gcgtgtcatc atccgtcgct
    40141 gcaatcgcca gccagcaggt gtcgagaagg ctttcatcaa atggcccttc gacgagggtt
    40201 aacatgcctg catctgccca tgcggtgaac tgtggaataa acgctaatgc attcaccgtt
    40261 aagcgagcgc ctgcgtctaa cagcaacctt gctttgcgtt ccgcgacatc accaccgccg
    40321 acaatcagac agtcgcgatc gcgtaattgg caaaatatag gcaaatgatc cacgtaaaaa
    40381 ccccttagta attaaccggc agctgtttca gtttgattaa atttgtccgc aaccggacga
    40441 ttcgctttcg gcgtagcata ccaataaccc aatcccatga atacggcacc tgataaagta
    40501 ttacccagcg tcacccacag caggttatga ccaatacccg ccagcgtgta ggcttcgctg
    40561 tggttgccga accaggagag cgcgaacagc gtcatgttag cgatagagtg ctcgtagccg
    40621 gacgcgataa atgccagcag acaccaccag atagcgataa atttcgccgc cccttcagtg
    40681 cgcagcgcca tccagattgc taggcaaacc agccagttac acaatgcacc tttgaagaac
    40741 agtaccattg ccggtgcagt ggttttagcc agcgcgacgg agtgaacgat gctggtatct
    40801 accggcagca ggctaccgcc gccccagcta tagagcatgg caacgaagac ggaaccgacc
    40861 aggttaccca gccaggtttg cggcaggatt gcccacattt gcccgtggct gatgctgccc
    40921 gcttttaccc caaaggtgag gaacatggtg tgtccggtga acagttcaga accggcgata
    40981 atcaccagcg ttaaggcgat accaaaggtc gcgcccatca ccagcggacg tacggacggg
    41041 tcgagcaaat taccgagcgt gaaaatcagg atgatcccaa gacccacata tgcgcccgcc
    41101 atggcggagc tgacccaaaa gccgagcggg ttatttgccg acaggcgtgc aatgcgcgca
    41161 gcattagccg cacacttatt aatagtgtct gtaaacattt gattatcctt taaaaaataa
    41221 aaacaaaaaa taaaacaaaa agtttaaaaa atgtcctgca atataccgaa ggtcattaca
    41281 ggaccatgca aaaaggggag gcattgcgcc tcccgttaaa acattaaccg cgcagctgca
    41341 ccacgccgtc tttcactcgt gcttcgtaat gtttgacgga aaactgttcg tcttccatgc
    41401 acaagccatc acttaagcga aaacgctgtt ttttcagcgg gctggcgacc cacagctcgc
    41461 cctggtgttc cgcaatcagc ccgcgtgata gcacgctgga ctcgaagaac ggatcaatgt
    41521 tgctgatcgc aaacacctgg tcgctgtgat acgggcggaa aatcgctacc tgcttgtcac
    41581 ctaacagtgc gcagacgccg gtttcaggca ggatgtcatc gattttgcag atgtctttcc
    41641 actggctcat gcgttgtcct ccaccagagt taccgggata cgttcatacg gcgttgccgg
    41701 acggtgctgt tcgcgctctg gcaccatctg cacgttcggg tcacgcttgt cgctgttgat
    41761 gaagtgtttg aagcgagtct gcgcggacgg tgtattgacc gtttcagtcc actcacacac
    41821 taccgcttca cgcaggcgcg ccatctcttc ttccagatga gcgttcagcc ccagtttgtc
    41881 gtcaatgatc accgctttca ggtaatcgat gccgccttcg aggttttcta accacggcgc
    41941 ggtacgcgtc agtttgtcgg cggtacggat gtagaacatc atgaagcggt cgagatattt
    42001 gatcagcgtt tcgcgatcga tatccgctgc cagcagatcc gcatgacgcg gtttcatgcc
    42061 gccgttaccg caaacataca ggttccagcc tttttcagtg gcgataatac ccacgtcttt
    42121 accctgagct tctgaacatt cacgggtaca gccggagaca ccgaacttca ttttgtgcgg
    42181 cgtacggatg cctttgtagc ggttttccag ttccacgccg aggccgacgc tatcgccaac
    42241 accgtagcgg caccaggtgc tacccacgca ggttttcgcc atacgcagtg ctttcgcata
    42301 ggcatgaccg gtttcgaagc cagcttcaat cagctgacgc cagatttccg gcagatcgtc
    42361 tttctgtgcg ccaaacatcg ccaggcgctg ggagccggtg attttggtgt agagattaaa
    42421 ttcacgcgcg atacgaccca ctgccatcag cccttccggg gtgatttcac cgcccggaga
    42481 acgcgggatc accgagtagg tgccgtcttt ctggatgtta gcgaggaagt tgtcgttaga
    42541 atcctgcagc ggagtatgtt ccggcttcag aatgtattcg ttccagcagg aggccagcag
    42601 cgaaccgacg gttggtttac aaacttcaca accgtagcct ttgccgtgtt tcgccagcag
    42661 ttcttcgaag gttttaatgc cttcaacgcg gatcaaatgg aacagttcct gacgcgaata
    42721 agcaaagtgt tcgcacaggt tgttgttaac ttcgatgccc tgtttcgcca gttccgcgtt
    42781 cagtacctga gtgaccagcg ggatacagcc accgcagcca gtacccgctt tggtttcagc
    42841 tttcagcgcc gcaactgtgt ggcagccttt gttgatggca gcaatcagat cacctttagt
    42901 gacgtcgaag caggagcaga tttgcgcgct gtccggcagt ttatcaacac cgatagacgg
    42961 cttaccgcta cccgagtgcg ccggcagaat cagagaatcc gggttttccg gcagctcgat
    43021 agcgttcagc accagttgca gcaggttgcc gtagtcgctg gtatcgccca ccagtaccgc
    43081 accgagcagg gttttgttgt cttcgctgac aatcaggcgt ttgtagatct ctttactttc
    43141 gtcgaggtaa acatagctac gtgcgccagg cgtgcgaccg tgcgcatcac caataccacc
    43201 tacgtctacg cccagcagtt tcagcttggc gctaaggtca gcaccttcaa aggcgttttc
    43261 gctacccaga atatggtcaa cggcgacctg cgccattttg tagcctggtg ctaccagacc
    43321 aaatacacgg ttgttccagc ttgcgcattc accgatggcg tagatatccg gatcggaagt
    43381 ctggcaggaa tcattaatga caatcccccc acgcggagca acgtccagac cacactgggt
    43441 tgccagctta tcgcgcggac ggataccggt agagaagacg ataaagtcga cttccagttc
    43501 gctgccgtcg gcaaaacgca tggttttacg cgcttcgaca ccttcctgca caatctcaag
    43561 ggtgtttttg ctggtgtgaa cgcgcacgcc catactttcg attttgcgac gcagctgctc
    43621 gccacccatc tgatcaagct gttccgccat cagcataggg gcaaattcga taacgtgggt
    43681 ttcaatacct aagtttttca gcgcgccagc ggcttccaga cctaacaggc cgccaccaac
    43741 aacggcaccg cgtttgctgc gacgggcgca ggattcaatg gcgttgaggt cttcaatggt
    43801 gcgatagaca aagcagtcct gagtatcaga acctttgatt ggtgggatcc acgggtagga
    43861 accggttgcc atgatcagct tgtcataaaa aacggtacgt ccggcgctgg agtgaatcac
    43921 cttctcctga cggttgatgg tgatagcgcg ttcgccgacc agaactttga tgccgtgttt
    43981 ctcgtagaag ccttcgcgca ccagcgacag ctcttcggcg gtgtggtgag agaagtaaga
    44041 cgagaggtgt acgcggtcat aagcgatgcg cggttcttca cagaaaacgg taatatcaaa
    44101 gttggccgca tcagatttat caagaagatc ttcgataaag cgatggccga ccataccgtt
    44161 accgataatt gcgagtctga ctttgctcat ttttgcctcg atttcttttc tattaccgcc
    44221 taccttaacg attcagcaac cccgcttatt gatgtaaatc aaattcacct ttatatactc
    44281 cttaaggggt atattgctga tttgtataaa ttttcttaag tcacggaatt gtctgacatt
    44341 tcatgtttgt gcaaaaaatg gacaaaatga acgggtttat agaaatagat gcacgtagcg
    44401 ggggaagaga ggaaaaggcc ggacgacgat agtgccgtcc ggcattagca attaatgaga
    44461 ggtcagggtg ttatgctgac ggtgacggct gacgaaacct aacaggaagc acatcacaaa
    44521 gacgacagcg tacagaccgt ttgctgtaag cagtgccgcc tgcggaccgc tatgttcaac
    44581 aatcgggccg gtaaccacaa aggtcaacat agtaccgatg gttccgcagg tcaggacaaa
    44641 gttaaccagt tttggcgacg gtactttggt ctgctgtgaa cccagggtga tgatggtggt
    44701 atagatcgcg ctggagaaga agcccagagc gagaattgac cacgccatat gtactggtgt
    44761 tccggtgtta aagacgtaca tcagaatcgc agccagacca gccagtacgg tcagaatgcg
    44821 ttgcaaatca aagaagcgaa gaataaagct gaacgcccac atgccgacca tgtatgacat
    44881 ccagaagtta ctcaccagcg tgcccgcgtc gttcaggctc atgcccaggc ctttggcata
    44941 ctcaggcacc caggagataa aacctaactg accgaggatg tagcacagcg ccgcaacaga
    45001 gagaaacagt acgccgatcc cccacttttc tttctctacc ggagcatccg tttttggtgc
    45061 acgtttgccc agtgccggga actcacagcc gaaggtcaga ataaaaatag cgacatacac
    45121 cagcccgatg caggcataaa cccagtacca ctcaatgctg cgcgccagta gaaacgcggc
    45181 gatcattggg aaaatcatcc cagccatact gaagaaggag tcagtaaata acaggcggga
    45241 accgcgctga cgcccttcat acatttgtgt tatcaggaat gtaccaatcg acatagtaat
    45301 gccgctgacc accccgagaa tgaacatcgc cgtcgagaac agcgccaggc tgtggctgaa
    45361 catcaaaccg gcaaccgcca gcaccatcag gagaaagcca aaacgtaact gcgttttcaa
    45421 cgggacgatt tccatcagcc aggcgttgag gaagatagag attaaaatgc cggcgttgag
    45481 gaaggtgaag gtgttactca tactggaaac aggcagattg aaataatcgg cgatatttcc
    45541 catcaccatc ccggtgacaa taaccaacgc accagtcagt gcgtaggaga gaaagctaat
    45601 ccatgtgagc ttgatgcgat tgctgttagt catgattggc ctgcgttcaa aaataaaatg
    45661 gcatagcggg atatgccgcg agcgggcgat tttaggtgat tttatgatct gtttaaatgt
    45721 tttattgcaa tcggttgcta aattgcattt taagaggtga ttttgatcac ggaataaaaa
    45781 gtgatcgtca ggttacatat atttcagata cgtaaaatta ggtaaaggga tggccttgtt
    45841 cttgaaggct atttagaatc tcttcacttg ctttttttct gctctgtttg ttaaggaaat
    45901 ctcatgttca aatcgaccct ggcggcgatg gctgctgttt tcgctctttc tgctctttct
    45961 gctctttctc ccgcagcaat ggcagcgaaa ggggacccgc acgtattgtt gacaacctca
    46021 gctggtaaca tcgaactgga gctggataaa caaaaagcgc cagtgtctgt gcaaaacttt
    46081 gtcgattatg tgaacagcgg tttttataac aacactacct ttcaccgcgt cattcctggc
    46141 tttatgattc agggcggcgg tttcaccgag cagatgcagc agaaaaaacc aaacccgcca
    46201 atcaaaaatg aagccgataa cggcctgcgc aacacgcgtg gcaccatcgc gatggcgcgt
    46261 accgctgaca aagacagcgc caccagccag ttctttatca acgttgccga taacgccttc
    46321 cttgaccatg gtcagcgtga tttcggttac gcggtatttg gtaaagtggt gaaaggcatg
    46381 gacgttgccg ataagatttc ccaggtgccg actcatgatg ttggtccgta ccagaatgtg
    46441 ccgtcaaaac cggtagttat cctttccgct aaagtcctgc cgtaatgatt tctcgcgcgg
    46501 gcaatcttgc ccgcgcttct gctctcccgg cgtaacccgg atttgccgct tatacttgtg
    46561 gcaaatggac acgttcaggg aggcatcaag tgaagaaact caccgataag caaaagtccc
    46621 gtctctggga gcttcagcgt aatcgtaatt tccaggccag tcgccgtctt gaaggcgtcg
    46681 agatgccttt agtcactctt actgccgcag aggctttagc gcgccttgaa gagctgagga
    46741 ggcactatga gcgataaatt cggcgaaggg cgcgatccgt atctttatcc tggccttgat
    46801 atcatgcgta accggctgaa catccgccag cagcagcggc tggaacaggc cgcttacgaa
    46861 atgacggcgc tgcgtgctgc gaccattgag cttggtccgc tggtgcgcgg tttaccgcat
    46921 ttgcgcacta tccatcgcca gctgtaccag gatattttcg actgggcagg gcaactgcgt
    46981 gaagttgata tttatcaggg cgatacgccg ttctgccact ttgcttatat cgaaaaagag
    47041 ggcaatgccc tgatgcagga tctggaggaa gaaggttatc tggttggcct ggagaaagcg
    47101 aagttcgtcg agcggctggc gcattactat tgtgaaatca acgtgctgca tcccttccgg
    47161 gtgggaagtg gtctggcaca gcggatcttc ttcgagcaac tggcgattca tgccggatat
    47221 caactgagct ggcagggtat cgaaaaagag gcctggaatc aggcaaatca gagtggggca
    47281 atgggggatc tcaccgcact gcagatgata tttagcaaag tggtaagcga agccggggaa
    47341 tctgagtaaa atagcgcggt tctttcgtac cggagccgcc atgatcctgc ttatagataa
    47401 ctacgattct tttacctgga acctctacca gtacttttgt gaactggggg cggatgtgct
    47461 ggttaagcgc aacgatgcgt tgacgctggc ggatatcgac gcccttaaac cacaaaaaat
    47521 tgtcatctca cctggcccct gtacgccaga tgaagccggg atctcccttg acgttattcg
    47581 ccactatgcc gggcgcttgc cgattcttgg cgtctgcctc ggtcatcagg caatggcgca
    47641 ggcatttggc ggcaaagttg tgcgcgccgc aaaggtcatg cacggcaaaa cctcgccgat
    47701 tacacatagc ggtgagggcg tatttcgggg gctggcaaat ccacttaccg tgacacgcta
    47761 ccattcgctg gtggtggaac ctgactcatt accagcgtgc tttgacgtga cggcctggag
    47821 cgaaacccga gagattatgg ggattcgcca tcgccagtgg gatctggaag gtgtgcagtt
    47881 ccatccagaa agtattctta gcgaacaagg acatcaactg ctggctaatt tcctgcatct
    47941 ctgatttccg attgccattc agtgattttt tatgcatatt ttgtgattat aatttcacat
    48001 ttatttatgc gtaacagggt gatcatgaga tggcaattga acaaacagca attacacgcg
    48061 cgactttcga tgaagtgatc ctgccgattt atgctccggc agagtttatt ccggtaaaag
    48121 gtcagggcag ccgaatctgg gatcagcaag gcaaggagta tgtcgatttc gcgggtggca
    48181 ttgcagttac ggcgttgggc cattgccatc ctgcgctggt gaacgcgtta aaaacccagg
    48241 gcgaaactct gtggcatatc agtaacgttt tcaccaatga accggcgctg cgtcttgggc
    48301 gcaaactgat tgaggcgacg tttgccgaac gcgtggtgtt tatgaactcc ggcacggaag
    48361 ctaacgaaac cgcctttaaa ctggcacgcc attacgcctg tgtgcgtcat agcccgttca
    48421 aaaccaaaat tattgccttc cataacgctt ttcatggtcg ctcgctgttt accgtttcgg
    48481 tgggtgggca gccaaaatat tccgacggct ttgggccgaa accggcagac atcatccacg
    48541 ttccctttaa cgatctccat gcagtgaaag cggtgatgga tgatcacacc tgtgcggtgg
    48601 tggttgagcc gatccagggc gagggcggtg tgacggcagc gacgccagag tttttgcagg
    48661 gcttgcgcga gctgtgcgat caacatcagg cattattggt gtttgatgaa gtgcagtgcg
    48721 ggatggggcg gactggcgat ttgtttgctt acaggtacta cggtgtgacg ccggatattc
    48781 tgacttctgc gaaagcgtta ggcggcggct tcccgattag cgccatgctg accacggcgg
    48841 aaattgcttc tgcgtttcat cctggttctc acggttccac ctacggcggt aatcctctgg
    48901 cctgtgcagt agcgggcgcg gcgtttgata tcatcaatac ccctgaagtg ctggaaggca
    48961 ttcaggcgaa acgccagcgt tttgttgacc atttgcagaa gatcgatcag cagtacgatg
    49021 tgtttagcga tattcgcggt atggggctgt taattggcgc ggagctgaaa ccacactaca
    49081 aaggtcgggc gcgtgatttc ctgtatgcgg gtgcagaggt tggcgtaatg gtgctgaatg
    49141 ccggaccgga tgtgatgcgt ttagcaccgt cgctggtggt ggaagatgcg gatatcgatg
    49201 aagggatgca acgtttcgcc cacgcggtgg cgaaggtggt tggggcgtaa ataagacgta
    49261 tcaggcaaaa tatgtcggat gcggcgtgaa tgccttatcc gatggtttag cctgtattac
    49321 gccttcgaat cccgcaactt gcgactcagc caaatcccgt gatgcggtcg ctgacgccat
    49381 gccatcgacg aaatggtgtg catggtgttc agatggccaa taacccgctg taaatgctgc
    49441 tccagcgtac ctgccgcgcc ttcgtgcggc tgcatctccg gcgcatccat gatattggca
    49501 tcgccagaac tacccggttc gtcatactcc agtcgctgct gacaacgctg aatggcgatt
    49561 tcacaagact gtaaatactc ttgtgccagt tcaggtggca atgcccggtg ttcccgcgcc
    49621 agcgtggtca tggcattaat atgctcaaca ataaactggc tgtgcgttac ccacagtttc
    49681 atatctgcca gataatggct gttgaatgcc ggttcctgca tcgcctgatt caatgagtta
    49741 tacagagtgt tatgcgcctg atttacccgc attctctgcc aggccagtgg cgttggttgc
    49801 gggtcctcgc tcagaatcaa gcgaatcgct tcctgatagg cttctaaagc atcatgggcg
    49861 tttttacgca ataacccgct ctgccactgc ggccacagcc agacggtacc accgaaagca
    49921 atcaaacaac caataatggt atcgataaga cgtggaagga tgtactgctc accgttcagc
    49981 cacaacagtt gcagggtata cactgcggta atagtaaaac cgaccgttgc ccagccgtag
    50041 tttttgcgca atatcaggta gctggcgagg gtggtaatca gcatcaacgt cagggtgtaa
    50101 ctttcgggaa ttttaaagtg cagcgctacg cccgcaatga ttaacccgac cacggttccc
    50161 acggagcgat tcacaatcct cagacgggtt gcgccatagc cattttgtgt caccagcaat
    50221 accgtcatca ggatccagta cgacttcggc agatgcagca cggtgcccat caggctggca
    50281 acgcttaaca tcacactgag tcgtccggca ttgcgtagcg ccggagactt tagtgacaga
    50341 taacttttca gtgccggaag caacggcatt cgccgctgtt tatcggccag taagtcacgg
    50401 gcatagagcg gtttttgggt gcgcagcacg cgggcgatgc ggctgaaatg ccagtagcag
    50461 aattgcccaa ccggattatc cggatgctgg cgggcgattt tttccagtgc gccaatttgc
    50521 ttttccatcg taaaacgcgt tggcaggcga tggtaaagaa tgtcatcagc cagcacgcgc
    50581 aggcgagcgg cgacggtttg cgcattccag cggataactt cttccgcatg gctacgctcg
    50641 accagttttt gcacctcttc cggctgatgc aaactgaccg aaatatgttc ctgcaaatcc
    50701 agcgcctcct ggaaaatacg cagcatccgc ttgtaatcgg tattgttttg cgcggaaagc
    50761 atatgcattt gctgatagca ctgagtaatt aaatcgaccg ctttttgctg gcgcaccagc
    50821 agcggtggca gcgctttttc aggatcggtg tgctgggtaa gcaggctgta tttggcttca
    50881 caataatctg ccagttcacg gtacagcaga cttagtgact cgcgcagcgg ttgttcgcgc
    50941 cagatccaga accaaaacca gttaaacaat ccgtaccaga gagtgcccaa cgcatagatg
    51001 agcaatggtt cccagaccgg catgtatccc gccagactga gggtaaaaat ggcggcgagc
    51061 agcgatgcag gaagcaattt tgcgtgcaat ggccccagct cagcagtgac gccaagtacc
    51121 agcgttaatc cggtcagcaa aaagggcagg ggaacatctt ttaccagtag cagctgtgtc
    51181 agtaagctac aggtggcaaa cagcgacgca ccaatgatta agtgtttgaa aaaacgttta
    51241 tgaggcgtat caaggcccgc aatattgcaa caggcaggaa cgagggagaa gagcagaccg
    51301 aatcgtaatt cgccaagcat taacccaacg gccacgggca aacatagcac cagcgtttgt
    51361 cgaagtgcat agttgatatc ggggtgataa atcagtctgc gccacatcgg gggaaacaaa
    51421 atggcgcgct accaggtaac gcgccactcc gacgggatta acgagtgccg taaacgacga
    51481 tggttttacc gtgtgcggag atcaggttct gatcttccag catcttcaga atgcgtccca
    51541 cggtttcacg agaacagccg acaatctgac cgatttcctg acgggtaatt ttgatttgca
    51601 taccgtccgg gtgagtcata gcatctggtt gttttgccag gttcagcaga gtctgtgcaa
    51661 tgcggcccgt cacgtcgagg aacgccaggt tgcccacttt ctctgaagtg acttgcagac
    51721 gacgcgccat ctgtgcagac agacgcatca gaatgtccgg gtttacctga atcaattggc
    51781 gaaatttttt gtacgaaatt tcagccactt cacaggcggt tttcgcacgt acccatgcgc
    51841 tacgttcctg gccctcttca aacaggccca gttcgccaat aaaatcaccc tgattcagat
    51901 aggagaggat catttcttta ccctcttcgt ctttgatcag cactgccaca gagcctttaa
    51961 cgatgtagta cagcgtttcc gctttttcac cctggtgaat aagcgtgctc ttggatgggt
    52021 acttatgaat gtggcaatga gacaagaacc attcgagagt cgggtctgtt tgcggtttgc
    52081 caagcaccat gcgcggttat cctctgttat aagctttctc cagagccaga taacgccgct
    52141 gtctctggat tgccgaaata tgcttcccgc tacctgggaa ggggctatca actgtactgc
    52201 acggtaatgt gacgtccttt gcatacatgc agtacatcaa tgtattactg tagcatcctg
    52261 actgttttag catagctttc gctttgtgtc tcctggtgtc tcgcttcagc atgacccagg
    52321 tcgccttccg ttgcgcgatt tggttagtac gcgtactctg tcaggaaaat tgacgcagtg
    52381 gagtagcaaa aatgcaagcg cgagtgaagt gggtcgaagg gttaactttt ctgggcgaat
    52441 ccgcctctgg tcaccagatt ttaatggacg gcaactcagg cgataaagca ccaagtccga
    52501 tggaaatggt gttgatggcg gcgggtggtt gcagtgccat cgatgtggtt tcgatcctgc
    52561 aaaaagggcg tcaggatgtg gtcgattgtg aagtaaaatt gacctctgaa cgccgcgaag
    52621 aggcaccacg cctgtttacg cacattaatc tgcattttat cgtcaccggt cgcgacctga
    52681 aagacgcagc ggttgcgcgt gcggttgatc tctctgccga gaaatattgt tcagtggcgt
    52741 tgatgctgga aaaagcggtg aatattactc actcgtatga agtggttgcc gcgtgactta
    52801 actatcccgt tgtgaagccg gatagtattt tatccggcat aatagcaaag ttactcgatt
    52861 ttctttcctt ccatcaatcg ttgcaccagc ggcagcataa ttaattccat tgccagcccc
    52921 attttaccgc caggtaccac taacgtattg atgtgggaaa tgaatgagcc ttgcaacatc
    52981 gccagcagcc aagggaaatc gatcccttcc agattacgaa aatggatcac cacaaagctt
    53041 tcatccagcg acgggatacc ttttgccgcg aacgggtttg aagtgtcgac ggtgggaacg
    53101 cgctggaagt taagatgggt gcgggaaaac tgcggtgtga tgtagttgat atagtcttcc
    53161 attgaacgca ctaccgagtc catcactgct tctcgtgagt gcccgcgctc gctggtgtcg
    53221 cggatcagtt tttgaatcca ctcaaggtta acgataggca ctacgccgac cagtaagtcc
    53281 acatgctgcg caacgttatg ctgtggcgtg actacgccgc cgtgtaaacc ttcataaaac
    53341 agtacatcag tgggttctgg taaaggttgc cagggggtga atgttcccgg tacctgattc
    53401 cagggtacgg cttcgtcgta ggtatgcaga tatttgcgag atttcccttt gccgctctga
    53461 ccgtattcaa tgaaggtttg ttccagcagg ccgaagtcgt tcgcttcggg gccgaagtag
    53521 ctgatatgcc gcccggcgtc gcgcgctttg cggatcgcca tgtccatttc cgggcgggtg
    53581 taacggtgaa aactgtcgcc ttccacctca gctgcatgca gatttaactg cgcgaatatt
    53641 ttacgaaacg cgaggctggt ggtggtggtc cccgcgccgc tggatcctgt taccgcaatg
    53701 accggatgtt tggcagacat agtaactcca tgacttagct atcactatta tctcgtccgc
    53761 cgacggttgc aggttattcg cgaaactgac tgcgcggcat aatattgacc gtttcgtgca
    53821 gctccgacca taccagcacc gcttctccgc actgtagctg gcgtttgacg tcggcgactt
    53881 tctgttcaag tgtacgctca tgttcaccat aatcggtgcc ttcacgtaac acaaagcttt
    53941 caattaaatt ttccagcgtt tcgggggaga ggtcttgcca cggaatcaac atgatttcgc
    54001 ctccagatac gttgttaacc agtcaggaat gcgtgactcc agccacattt gcggatgaag
    54061 taatgtaccg ccaataaagc caacatgacc gccatgttca gtcagttgat actccacctg
    54121 cgaggggaga ctttccggtt ttgggatcac ctgatgatcc ataaacggat cgtctttggc
    54181 gtgaataatc agcgtcggtt tggcgatccg gttcagcatt ggcatggcgc tacactgacg
    54241 ataatagtcg atagcgtcgg cgtagccgtg aattctggcg gtgattagat cgtcaaattc
    54301 acggatgcga cgtaccgatt ttaactgcgc gagattaatc ggcagcgttc cggggtaggc
    54361 tgccagcttg cgcgcggcat tggcttttaa caggttcagc aagtaacgct gataaacgcg
    54421 ggaaaagccc ttttccatat gatagctaca ggcttccagc ataaacggcg cagagacaat
    54481 caccgccgca tcaaccggga gatcattgcc ttcctttgcc agcaaacagg ccagcatatt
    54541 accgccgagc gaatagccga cggcagccgt tggcgcatga ccaaattcgc gttgcagcca
    54601 gcgtaaaaac caactggcgt cttcggtttc gcccgaatgg taaatgcggt gcatacggtt
    54661 tggttcaccg ctgcatccgc gaaaatgcat caccacgccc agccagccgc gcttttgcgc
    54721 cgcctcaacc agaccgtggg cgtaagggct attgagactg ccttccagcc cgtgaaacac
    54781 cactaaacgc ggtttatgtt tcgcctgtgc ggggtcttca ctccacgcga gatcgacaaa
    54841 atcgccgtcg ggcaactcca gccgctgcca gtacggggtg aatttcacct gacgacgaaa
    54901 caaacgcggc agcatagttt gtagatgaca attgctaaag ccgcgcatag gggtgaattc
    54961 agcactgctg ctaaattcat tggcatcggt cgtcgttatc tgcgccatca gttgctttgg
    55021 ccttccagca gcatctgctc aagctgctcc tgggcttcca gccacgccat ttcgcactct
    55081 tccaggccgg atttggcgct ggcttgctgt tgcaggcagg cggtcaactc cgctttacgg
    55141 ctctggtcat acagttcgct gtcgccgagt ttctcttccg cctgcgccag ttgcgcgttc
    55201 agcttctcca tctctttttc cagacgggca atctctttac gcagcggctg ggtttgcgca
    55261 cgcagctccg cttcccgacg cttctgatct ttacgtgcct gggcgctgtt cgcgttctct
    55321 tttggcgctt cgtcggtctg gttttcctgc ttttgtacgt cgctcaacca ctgttgataa
    55381 tcttccagat cgccgtcgaa cggttcgact ttacgatcgt gaaccaggta gagatcgtca
    55441 gtggtggaac gcagcaaatg acggtcgtgc gaaacgacaa ccagcgcgcc ttcaaactcg
    55501 attaatgctt cggtgagtgc ctgacgcatg tcgaggtcaa ggtggttagt cggttcgtcg
    55561 agcagcagca gattcggacg ctgccagaca attaatgcca gcaccaggcg ggctttttcc
    55621 ccaccggaga agcggcgcgt ttcttcggtt actttatcgc ccaggaaacc aaagccgccg
    55681 aggtagtcac gcagtttttg ttccagctcc tgcggcgcta aacgtgccag atgttgaata
    55741 ggtgattcgt cggcgcgcag gtattcaagt tgatgctggg cgaagtagcc gagcttgatc
    55801 cctttcgcca gaccaatttc accgctgact ggcgcaagtt caccggctaa cagtttgatt
    55861 aatgtcgatt tacctgcgcc gttgcggcct aacagaccaa tgcgcgagcc ggggaccaga
    55921 ttcaatttaa tcgagtcgag aataatgcga tcgccatagc ctgcgctgac cttttccatc
    55981 ttcagtaacg gatttggcag gctttccggc gcgcggaagc taaagcggaa cgggttgtcg
    56041 acgtgtgcgg gggcaatcag ctccatacgt tccagcatct taatgcggct ctgggcctgc
    56101 ttcgctttgg tggctttggc acggaaacga tcgatataac tttgcagatg tgccacgcgt
    56161 tcctgctggc tttcgtacat cgcttgttgc tgcgccagac gggtggcgcg ctgtacttca
    56221 aacgaactgt agttgccggt gtactcgaac atgctttgtt gttcgatatg aataatttta
    56281 tcaacgatcg gatcgaggaa gtcgcggtcg tgagagatca ggatcagcgt gccctgatag
    56341 ctcttcaacc atttttccag ccagataacg gcatcgagat cgaggtggtt agtcggttcg
    56401 tcgagcagca gcaagtctga acggcaaatc agcgcctggg caaggttaag acgcatacgc
    56461 cagccaccgg agaaatcact taccgggcgc tccagttgtt cattgctgaa accgaggccg
    56521 tgcagcaggc tggcggcacg ggagcgaata ctccatgcgt caatagcatc cagcttgcta
    56581 tgaatggtcg caatggcgtg cccgtcgtta cgttcgttgg cgtcgtgcag ctgcgcttcc
    56641 agttgacgat attcacggtc gccgtcaatg acatattcca gcgccgcttg cggtaacgct
    56701 ggcgtttcct gattcaccca cgccagttgc cagcttcccg gaaaggtgta gctgccgccg
    56761 tcggcgctga tttcattttt cagcaatgcc agcagggtag atttaccaca gccgttttta
    56821 cccaccaggc cgactttctg cccggggttg atggtggcgg tggcattatc cagcaggacg
    56881 cgcacgccgc gacgaatttg taacgaggag aaaacaatca taaggcgccg tatgttcaga
    56941 ctatgttaac ttatcattat gataatgtaa tgtatgggcg agctgccgca ccgggcgcaa
    57001 tggtagccca aaacagcgac tatacacaaa aaccataccg ggagggggat gatgtctcag
    57061 ccagcgaaag ttttgctgct gtatgcccat ccggaatctc aggactcggt ggcaaaccgg
    57121 gtactgctta aaccggccac gcagctcagc aatgttaccg tgcacgacct ttacgcgcac
    57181 tatcccgatt tttttattga tatcccccgt gagcaggcat tactgcgcga gcacgaggtg
    57241 attgtctttc agcatcctct ttatacctat agctgcccgg cgctactgaa agagtggctg
    57301 gaccgggtat taagtcgtgg ttttgccagc gggccgggag gaaaccaact ggcgggaaag
    57361 tactggcgta acgtgattac caccggcgag ccggaaagtg cttaccgtta tgacgcgctg
    57421 aatcgctacc cgatgagcga tgtgctgcgc ccctttgaac tggcggcggg catgtgccgg
    57481 atgcattggt taagtcccat cattatttac tgggcgagac ggcaaagcgc aaaggagctg
    57541 gcgagccacg ccagagccta cggtgactgg ttggcaaatc cgctgtctcc aggaggccgc
    57601 tgatggaagg ttccgatttt ttactcgcag gagtgctgtt tctcttcgcg gctggggatt
    57661 ggcgctgtgt tgggatattt gctggcgggg attgcaattg gcccgtgggg gctggggttt
    57721 attagcgacg tcgatgagat cctccacttt tcggaactcg gcgtggtatt cctgatgttt
    57781 atcatcggcc ttgagttgaa tccctccaaa ctttggcaac tgcggcgttc gatttttggc
    57841 gtaggcgcgg cacaggtgct gttaagcgcg gcgttgctgg cgggattatt gatgctgacg
    57901 gatttcgcct ggcaggcggc ggtggtcggt ggcattggct ttgcgatgtc ttcaactgca
    57961 atggcgttgc aattgatgcg tgagaaaggg atgaatcgca gcgaatccgg ccagctcggg
    58021 ttttcggttc tgctgtttca ggatctggca gtgatcccag cactggcgtt agtgccgttg
    58081 ttggcggggt cggcagacga acatttcgac tggatgaaga tcggcatgaa ggtgctggag
    58141 tttgtcggca tgctgattgg tgggcgctat ttactgcgtc cggtattccg ctttattgca
    58201 gcttctggcg tgcgggaagt gttcaccgcc gcgacgctgc tgctggtgtt gggttccgca
    58261 ttgtttatgg atgcgctggg gctgtcgatg gcgctcggta catttattgc gggcgtgctg
    58321 ctggcggaaa gtgaatatcg ccatgaactg gaaacggcta tcgatccctt caaaggcttg
    58381 ctgctcggtt tgttctttat ctctgtcggc atgtcgctca acctcggggt gctttatacc
    58441 catctgttgt gggtagtgat aagcgtggtt gtgctggtgg cggtgaaaat tctcgtgctg
    58501 tatctgctgg cgcgattgta tggcgtgcgt agctcagagc ggatgcagtt tgctggcgtg
    58561 ttgagtcagg gcggtgagtt tgcctttgtc ctcttttcta ccgcttcttc acaacgttta
    58621 ttccagggcg accagatggc gccgttgctg atgaagctgg tggataaatg gctatcccgc
    58681 cagtttaacg gaccggaaga agaagacgaa aaaccgtggg tcaacgatga taaaccccag
    58741 gtcattgtcg tgggcttcgg gcgttttggt caggtgattg gtcgtttgct gatggcaaat
    58801 aaaatgcgca ttaccgtgct ggagcgggat atcagcgccg ttaacctgat gcgtaaatat
    58861 ggctacaaag tttattacgg cgacgccacg caggtcgatc ttttacgttc tgcgggtgca
    58921 gaggccgctg agtctatcgt cattacctgt aacgagccgg aagacaccat gaagctggtg
    58981 gaaatatgcc agcagcactt tccgcatttg catattcttg cgcgagcgcg cggacgtgtg
    59041 gaagcgcatg agttattaca ggcaggggtg acgcagtttt cccgtgaaac attctccagt
    59101 gcgttagagc tggggcgcaa gacgctggtc acgcttggca tgcatccgca tcaggcacag
    59161 cgggcgcaac tgcattttcg ccgcctggat atgcgaatgc tgcgggagtt aatcccgatg
    59221 catgctgata ccgtacaaat ttctcgcgcc agggaagccc gacgcgaact ggaagagatt
    59281 ttccagcgtg aaatgcaaca agaacgacgc cagctggacg gctgggatga atttgagtag
    59341 agggtaagga tggcaatccg aaaacgtttt atagcgggcg caaaatgccc ggcctgtcag
    59401 gcgcaggatt caatggcgat gtggcgcgaa aataatattg atattgttga atgtgttaag
    59461 tgcggacatc agatgcgaga agcagacaaa gaagcccgcg atcacgttca caaagatgag
    59521 caagtgatcg ggatttttca tccggactag cgatatgcgc cgagtttttt taagctagtg
    59581 agtacacggc tgcagaattc cgctacaatc tgcgccacta ttcttcccat gctcaggaga
    59641 tatcatgaaa gtagcaaaag acctggtggt cagcctggcc tatcaggtac gtacagaaga
    59701 cggtgtgttg gttgatgagt ctccggtgag tgcgccgctg gactacctgc atggtcacgg
    59761 ttccctgatc tctggcctgg aaacggcgct ggaaggtcat gaagttggcg acaaatttga
    59821 tgtcgctgtt ggcgcgaacg acgcttacgg tcagtacgac gaaaacctag tgcaacgtgt
    59881 tcctaaagac gtatttatgg gcgttgatga actgcaggta ggtatgcgtt tcctggctga
    59941 aactgaccag ggtccggtac cggttgaaat cactgcggtt gaagacgatc acgtagtggt
    60001 tgatggtaac cacatgctgg ccggtcagaa cctgaaattc aacgttgaag ttgtggcgat
    60061 tcgcgaagcg accgaagaag aactggctca tggccacgtt cacggcgcgc acgatcatca
    60121 ccacgatcac gaccacgacg gttgctgcgg cggtcatggc cacgatcacg gtcatgaaca
    60181 cggtggcgaa ggctgctgtg gcggtaaagg caacggcggt tgtggttgcc actaataccg
    60241 aaaaggtgac aaaaaagcgg ggaatccccg ctttttttac gcc
//
LOCUS       DADLDT020000009        55430 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000009 DADLDT020000000
VERSION     DADLDT020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.009"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQH22_000534"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQH22_000534"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFB2204979.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQH22_000535"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQH22_000535"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFB2204980.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQH22_000536"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQH22_000536"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFB2204981.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JQH22_000537"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JQH22_000537"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFB2204982.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JQH22_000538"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JQH22_000538"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFB2204983.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="JQH22_000539"
     CDS             7826..9559
                     /locus_tag="JQH22_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFB2204984.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JQH22_000540"
     CDS             9708..10559
                     /locus_tag="JQH22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2204985.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSRCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JQH22_000541"
     CDS             complement(10546..10887)
                     /locus_tag="JQH22_000541"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFB2204986.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JQH22_000542"
     CDS             complement(10889..11767)
                     /locus_tag="JQH22_000542"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFB2204987.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JQH22_000543"
     CDS             complement(11733..14030)
                     /locus_tag="JQH22_000543"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFB2204988.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLGTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JQH22_000544"
     CDS             complement(14081..14401)
                     /locus_tag="JQH22_000544"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFB2204989.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JQH22_000545"
     CDS             complement(14416..15495)
                     /locus_tag="JQH22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFB2204990.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="JQH22_000546"
     CDS             15804..16907
                     /locus_tag="JQH22_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFB2204991.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="JQH22_000547"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="JQH22_000547"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFB2204992.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JQH22_000548"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JQH22_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="JQH22_000549"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="JQH22_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="JQH22_000550"
     CDS             20366..20983
                     /locus_tag="JQH22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFB2204993.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="JQH22_000551"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="JQH22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFB2204994.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="JQH22_000552"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="JQH22_000552"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFB2204995.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="JQH22_000553"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="JQH22_000553"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFB2204996.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="JQH22_000554"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="JQH22_000554"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFB2204997.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="JQH22_000555"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="JQH22_000555"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFB2204998.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="JQH22_000556"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="JQH22_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFB2204999.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="JQH22_000557"
     CDS             30130..30738
                     /locus_tag="JQH22_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFB2205000.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="JQH22_000558"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="JQH22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFB2205001.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="JQH22_000559"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="JQH22_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFB2205002.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="JQH22_000560"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="JQH22_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFB2205003.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="JQH22_000561"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="JQH22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFB2205004.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="JQH22_000562"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="JQH22_000562"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFB2205005.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="JQH22_000563"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="JQH22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFB2205006.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="JQH22_000564"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="JQH22_000564"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFB2205007.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="JQH22_000565"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="JQH22_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFB2205008.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="JQH22_000566"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="JQH22_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFB2205009.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="JQH22_000567"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="JQH22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="JQH22_000568"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="JQH22_000568"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFB2205010.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="JQH22_000569"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="JQH22_000569"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFB2205011.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="JQH22_000570"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="JQH22_000570"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFB2205012.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="JQH22_000571"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="JQH22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFB2205013.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="JQH22_000572"
     CDS             complement(44853..45152)
                     /locus_tag="JQH22_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFB2205014.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="JQH22_000573"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="JQH22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="JQH22_000574"
     CDS             45905..46504
                     /locus_tag="JQH22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFB2205015.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="JQH22_000575"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="JQH22_000575"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFB2205016.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="JQH22_000576"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="JQH22_000576"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFB2205017.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="JQH22_000577"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="JQH22_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFB2205018.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="JQH22_000578"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="JQH22_000578"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFB2205019.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="JQH22_000579"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="JQH22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFB2205020.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="JQH22_000580"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="JQH22_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFB2205021.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="JQH22_000581"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="JQH22_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFB2205022.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="JQH22_000582"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="JQH22_000582"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFB2205023.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="JQH22_000583"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="JQH22_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HFB2205024.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13504 a        15021 c        14162 g        12743 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cgctgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gccaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DADLDT020000010        53210 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000010 DADLDT020000000
VERSION     DADLDT020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.010"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(11..322)
                     /locus_tag="JQH22_000584"
     CDS             complement(11..322)
                     /locus_tag="JQH22_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFB2205025.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JQH22_000585"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JQH22_000585"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JQH22_000586"
     CDS             701..1054
                     /locus_tag="JQH22_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFB2205026.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQH22_000587"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQH22_000587"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFB2205027.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JQH22_000588"
     CDS             complement(2810..3280)
                     /locus_tag="JQH22_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFB2205028.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQH22_000589"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQH22_000589"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFB2205029.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQH22_000590"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQH22_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFB2205030.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQH22_000591"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQH22_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFB2205031.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JQH22_000592"
     CDS             complement(5283..8432)
                     /locus_tag="JQH22_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HFB2205032.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQH22_000593"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQH22_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFB2205033.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JQH22_000594"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JQH22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFB2205034.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JQH22_000595"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JQH22_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFB2205035.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQH22_000596"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQH22_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFB2205036.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQH22_000597"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQH22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFB2205037.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JQH22_000598"
     CDS             14911..15288
                     /locus_tag="JQH22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFB2205038.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JQH22_000599"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JQH22_000599"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFB2205039.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JQH22_000600"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JQH22_000600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFB2205040.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="JQH22_000600"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="JQH22_000601"
     CDS             18099..18428
                     /locus_tag="JQH22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFB2205041.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JQH22_000602"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JQH22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HFB2205042.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JQH22_000603"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JQH22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFB2205043.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JQH22_000604"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JQH22_000604"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFB2205044.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JQH22_000605"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JQH22_000605"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFB2205045.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQH22_000606"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQH22_000606"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFB2205046.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="JQH22_000607"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="JQH22_000607"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFB2205047.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="JQH22_000608"
     CDS             complement(25481..27157)
                     /locus_tag="JQH22_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HFB2205048.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="JQH22_000609"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="JQH22_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFB2205049.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="JQH22_000610"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="JQH22_000610"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFB2205050.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQH22_000611"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQH22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFB2205051.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="JQH22_000612"
     CDS             complement(31205..31999)
                     /locus_tag="JQH22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFB2205052.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="JQH22_000613"
     CDS             32137..32478
                     /locus_tag="JQH22_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HFB2205053.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQH22_000614"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQH22_000614"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFB2205054.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAELQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIARVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="JQH22_000615"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="JQH22_000615"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFB2205055.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="JQH22_000616"
     CDS             36394..37686
                     /locus_tag="JQH22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2205056.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="JQH22_000617"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="JQH22_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFB2205057.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="JQH22_000618"
     CDS             complement(38219..38677)
                     /locus_tag="JQH22_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFB2205058.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="JQH22_000619"
     CDS             complement(38674..39591)
                     /locus_tag="JQH22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HFB2205059.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="JQH22_000620"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="JQH22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFB2205060.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="JQH22_000621"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="JQH22_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFB2205061.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQH22_000622"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQH22_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFB2205062.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQH22_000623"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQH22_000623"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFB2205063.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQH22_000624"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQH22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="JQH22_000625"
     CDS             43550..44236
                     /locus_tag="JQH22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2205064.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="JQH22_000626"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="JQH22_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JQH22_000627"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JQH22_000627"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFB2205065.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JQH22_000628"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JQH22_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFB2205066.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JQH22_000629"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JQH22_000629"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFB2205067.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JQH22_000630"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JQH22_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFB2205068.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JQH22_000631"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JQH22_000631"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFB2205069.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="JQH22_000632"
     CDS             52938..>53210
                     /locus_tag="JQH22_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFB2205070.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12829 a        13689 c        14020 g        12672 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actctggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagctccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DADLDT020000011        52075 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000011 DADLDT020000000
VERSION     DADLDT020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52075
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.011"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="JQH22_000633"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="JQH22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFB2205071.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="JQH22_000634"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="JQH22_000634"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFB2205072.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="JQH22_000635"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="JQH22_000635"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFB2205073.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTAQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="JQH22_000636"
     CDS             complement(5906..6949)
                     /locus_tag="JQH22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFB2205074.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="JQH22_000637"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="JQH22_000637"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFB2205075.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="JQH22_000638"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="JQH22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFB2205076.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="JQH22_000639"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="JQH22_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="JQH22_000640"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="JQH22_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFB2205077.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="JQH22_000641"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="JQH22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFB2205078.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="JQH22_000642"
     CDS             15474..15830
                     /locus_tag="JQH22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFB2205079.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="JQH22_000643"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="JQH22_000643"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFB2205080.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="JQH22_000644"
     CDS             16989..17189
                     /locus_tag="JQH22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFB2205081.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="JQH22_000645"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="JQH22_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFB2205082.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="JQH22_000646"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="JQH22_000646"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFB2205083.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="JQH22_000647"
     CDS             complement(20101..20964)
                     /locus_tag="JQH22_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFB2205084.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="JQH22_000648"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="JQH22_000648"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFB2205085.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="JQH22_000649"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="JQH22_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFB2205086.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="JQH22_000650"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="JQH22_000650"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFB2205087.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="JQH22_000651"
     CDS             24133..24705
                     /locus_tag="JQH22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFB2205088.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="JQH22_000652"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="JQH22_000652"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFB2205089.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="JQH22_000653"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="JQH22_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFB2205090.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="JQH22_000654"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="JQH22_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="JQH22_000655"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="JQH22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFB2205091.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="JQH22_000656"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="JQH22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFB2205092.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="JQH22_000657"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="JQH22_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFB2205093.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="JQH22_000658"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="JQH22_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFB2205094.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="JQH22_000659"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="JQH22_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="JQH22_000660"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="JQH22_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFB2205095.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="JQH22_000661"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="JQH22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFB2205096.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="JQH22_000662"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="JQH22_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFB2205097.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="JQH22_000663"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="JQH22_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFB2205098.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="JQH22_000664"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="JQH22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="JQH22_000665"
     CDS             complement(39099..40160)
                     /locus_tag="JQH22_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFB2205099.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="JQH22_000666"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="JQH22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFB2205100.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="JQH22_000667"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="JQH22_000667"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFB2205101.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="JQH22_000668"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="JQH22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFB2205102.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="JQH22_000669"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="JQH22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFB2205103.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="JQH22_000670"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="JQH22_000670"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFB2205104.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="JQH22_000671"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="JQH22_000671"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFB2205105.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="JQH22_000672"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="JQH22_000672"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFB2205106.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="JQH22_000673"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="JQH22_000673"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFB2205107.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="JQH22_000674"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="JQH22_000674"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFB2205108.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="JQH22_000675"
     CDS             complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="JQH22_000675"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFB2205109.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12155 a        13738 c        13522 g        12660 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacggctc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgctt
//
LOCUS       DADLDT020000012        49530 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000012 DADLDT020000000
VERSION     DADLDT020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49530
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.012"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="JQH22_000676"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="JQH22_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFB2205110.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="JQH22_000677"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="JQH22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFB2205111.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="JQH22_000678"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="JQH22_000678"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFB2205112.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="JQH22_000679"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="JQH22_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFB2205113.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="JQH22_000680"
     CDS             5718..6659
                     /locus_tag="JQH22_000680"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFB2205114.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="JQH22_000681"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="JQH22_000681"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFB2205115.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="JQH22_000682"
     CDS             7679..8929
                     /locus_tag="JQH22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFB2205116.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="JQH22_000683"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="JQH22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="JQH22_000684"
                     /pseudo
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="JQH22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10866..12116
                     /locus_tag="JQH22_000685"
     CDS             10866..12116
                     /locus_tag="JQH22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFB2205117.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="JQH22_000686"
     CDS             complement(12172..13260)
                     /locus_tag="JQH22_000686"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFB2205118.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="JQH22_000687"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="JQH22_000687"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFB2205119.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="JQH22_000688"
     CDS             complement(14194..15243)
                     /locus_tag="JQH22_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFB2205120.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="JQH22_000689"
     CDS             15342..16223
                     /locus_tag="JQH22_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2205121.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="JQH22_000690"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="JQH22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFB2205122.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="JQH22_000691"
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="JQH22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFB2205123.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="JQH22_000692"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="JQH22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="JQH22_000693"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="JQH22_000693"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFB2205124.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="JQH22_000694"
     CDS             21991..23067
                     /locus_tag="JQH22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HFB2205125.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="JQH22_000695"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="JQH22_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFB2205126.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="JQH22_000696"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="JQH22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFB2205127.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="JQH22_000697"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="JQH22_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFB2205128.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="JQH22_000698"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="JQH22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFB2205129.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="JQH22_000699"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="JQH22_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="JQH22_000700"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="JQH22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFB2205130.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="JQH22_000701"
     CDS             complement(30809..31048)
                     /locus_tag="JQH22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFB2205131.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="JQH22_000702"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="JQH22_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFB2205132.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="JQH22_000703"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="JQH22_000703"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFB2205133.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="JQH22_000704"
     CDS             complement(32934..33629)
                     /locus_tag="JQH22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFB2205134.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="JQH22_000705"
     CDS             complement(33626..34024)
                     /locus_tag="JQH22_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFB2205135.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="JQH22_000706"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="JQH22_000706"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFB2205136.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="JQH22_000707"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="JQH22_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HFB2205137.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="JQH22_000708"
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="JQH22_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFB2205138.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37377..38138
                     /locus_tag="JQH22_000709"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="JQH22_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="JQH22_000710"
     CDS             complement(38255..38833)
                     /locus_tag="JQH22_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2205139.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="JQH22_000711"
     CDS             39003..39590
                     /locus_tag="JQH22_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HFB2205140.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="JQH22_000712"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="JQH22_000712"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFB2205141.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="JQH22_000713"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="JQH22_000713"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFB2205142.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="JQH22_000714"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="JQH22_000714"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFB2205143.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="JQH22_000715"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="JQH22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFB2205144.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="JQH22_000716"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="JQH22_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFB2205145.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="JQH22_000717"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="JQH22_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFB2205146.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="JQH22_000718"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="JQH22_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFB2205147.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="JQH22_000719"
     CDS             complement(48911..49018)
                     /locus_tag="JQH22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFB2205148.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49530)
                     /locus_tag="JQH22_000720"
                     /pseudo
     CDS             complement(49078..49530)
                     /locus_tag="JQH22_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11954 a        12199 c        13310 g        12067 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ctagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgta gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gcggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagatc cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cacagaatgt ctgaattaaa
     6661 aatctcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgaccg gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgctaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcagata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtatg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagcag gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaatatt tggattgata
    18001 attgttatcg tttgcattat cgttacgaca caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agactctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ttggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcgggtct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gattgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atctgcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggatg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctcaggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc gactgcgacg
    34921 cagtgatgat tggtcgcggg gcgttcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagtcctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat tcagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagagc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattata cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatttgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatacacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatctttca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccggtgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttactggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc
//
LOCUS       DADLDT020000013        47978 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000013 DADLDT020000000
VERSION     DADLDT020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47978
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.013"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..76)
                     /locus_tag="JQH22_000721"
     tRNA            complement(1..76)
                     /locus_tag="JQH22_000721"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Val,seq:tac)
     gene            complement(128..203)
                     /locus_tag="JQH22_000722"
     tRNA            complement(128..203)
                     /locus_tag="JQH22_000722"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Lys,seq:ttt)
     gene            complement(206..281)
                     /locus_tag="JQH22_000723"
     tRNA            complement(206..281)
                     /locus_tag="JQH22_000723"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Val,seq:tac)
     gene            complement(418..493)
                     /locus_tag="JQH22_000724"
     tRNA            complement(418..493)
                     /locus_tag="JQH22_000724"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Lys,seq:ttt)
     gene            complement(658..1449)
                     /gene="cpoB"
                     /locus_tag="JQH22_000725"
     CDS             complement(658..1449)
                     /gene="cpoB"
                     /locus_tag="JQH22_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFB2205149.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1459..1980)
                     /gene="pal"
                     /locus_tag="JQH22_000726"
     CDS             complement(1459..1980)
                     /gene="pal"
                     /locus_tag="JQH22_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFB2205150.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2015..3307)
                     /gene="tolB"
                     /locus_tag="JQH22_000727"
     CDS             complement(2015..3307)
                     /gene="tolB"
                     /locus_tag="JQH22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HFB2205151.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3440..4681)
                     /gene="tolA"
                     /locus_tag="JQH22_000728"
     CDS             complement(3440..4681)
                     /gene="tolA"
                     /locus_tag="JQH22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFB2205152.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4746..5174)
                     /gene="tolR"
                     /locus_tag="JQH22_000729"
     CDS             complement(4746..5174)
                     /gene="tolR"
                     /locus_tag="JQH22_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFB2205153.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5178..5870)
                     /gene="tolQ"
                     /locus_tag="JQH22_000730"
     CDS             complement(5178..5870)
                     /gene="tolQ"
                     /locus_tag="JQH22_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFB2205154.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5867..6271)
                     /gene="ybgC"
                     /locus_tag="JQH22_000731"
     CDS             complement(5867..6271)
                     /gene="ybgC"
                     /locus_tag="JQH22_000731"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFB2205155.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6421..6714)
                     /gene="ybgE"
                     /locus_tag="JQH22_000732"
     CDS             complement(6421..6714)
                     /gene="ybgE"
                     /locus_tag="JQH22_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFB2205156.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6714..6827)
                     /gene="cydX"
                     /locus_tag="JQH22_000733"
     CDS             complement(6714..6827)
                     /gene="cydX"
                     /locus_tag="JQH22_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFB2205157.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6842..7981)
                     /gene="cydB"
                     /locus_tag="JQH22_000734"
     CDS             complement(6842..7981)
                     /gene="cydB"
                     /locus_tag="JQH22_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFB2205158.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7997..9565)
                     /gene="cydA"
                     /locus_tag="JQH22_000735"
     CDS             complement(7997..9565)
                     /gene="cydA"
                     /locus_tag="JQH22_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFB2205159.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10414..13047)
                     /gene="mngB"
                     /locus_tag="JQH22_000736"
                     /pseudo
     CDS             complement(10414..13047)
                     /gene="mngB"
                     /locus_tag="JQH22_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(13065..14981)
                     /gene="mngA"
                     /locus_tag="JQH22_000737"
     CDS             complement(13065..14981)
                     /gene="mngA"
                     /locus_tag="JQH22_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFB2205160.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15151..15873
                     /gene="mngR"
                     /locus_tag="JQH22_000738"
     CDS             15151..15873
                     /gene="mngR"
                     /locus_tag="JQH22_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFB2205161.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15976..16845)
                     /gene="sucD"
                     /locus_tag="JQH22_000739"
     CDS             complement(15976..16845)
                     /gene="sucD"
                     /locus_tag="JQH22_000739"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFB2205162.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16845..18011)
                     /gene="sucC"
                     /locus_tag="JQH22_000740"
     CDS             complement(16845..18011)
                     /gene="sucC"
                     /locus_tag="JQH22_000740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFB2205163.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18105..19322)
                     /gene="odhB"
                     /locus_tag="JQH22_000741"
     CDS             complement(18105..19322)
                     /gene="odhB"
                     /locus_tag="JQH22_000741"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFB2205164.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19337..22138)
                     /gene="sucA"
                     /locus_tag="JQH22_000742"
     CDS             complement(19337..22138)
                     /gene="sucA"
                     /locus_tag="JQH22_000742"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFB2205165.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22439..23155)
                     /gene="sdhB"
                     /locus_tag="JQH22_000743"
     CDS             complement(22439..23155)
                     /gene="sdhB"
                     /locus_tag="JQH22_000743"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFB2205166.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23171..24937)
                     /gene="sdhA"
                     /locus_tag="JQH22_000744"
     CDS             complement(23171..24937)
                     /gene="sdhA"
                     /locus_tag="JQH22_000744"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFB2205167.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24937..25284)
                     /gene="sdhD"
                     /locus_tag="JQH22_000745"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24937..25284)
                     /gene="sdhD"
                     /locus_tag="JQH22_000745"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFB2205168.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25278..25682)
                     /gene="sdhC"
                     /locus_tag="JQH22_000746"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25278..25682)
                     /gene="sdhC"
                     /locus_tag="JQH22_000746"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFB2205169.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26063..26170
                     /locus_tag="JQH22_000747"
     CDS             26063..26170
                     /locus_tag="JQH22_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205170.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26376..27659
                     /gene="gltA"
                     /locus_tag="JQH22_000748"
     CDS             26376..27659
                     /gene="gltA"
                     /locus_tag="JQH22_000748"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFB2205171.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28050..28616
                     /locus_tag="JQH22_000749"
     CDS             28050..28616
                     /locus_tag="JQH22_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFB2205172.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28667..31126
                     /locus_tag="JQH22_000750"
     CDS             28667..31126
                     /locus_tag="JQH22_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFB2205173.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            31141..31872
                     /locus_tag="JQH22_000751"
     CDS             31141..31872
                     /locus_tag="JQH22_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFB2205174.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31869..32945
                     /locus_tag="JQH22_000752"
                     /pseudo
     CDS             31869..32945
                     /locus_tag="JQH22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            33097..34143
                     /locus_tag="JQH22_000753"
     CDS             33097..34143
                     /locus_tag="JQH22_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFB2205175.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34140..34931)
                     /gene="nei"
                     /locus_tag="JQH22_000754"
     CDS             complement(34140..34931)
                     /gene="nei"
                     /locus_tag="JQH22_000754"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFB2205176.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34967..35701)
                     /gene="pxpA"
                     /locus_tag="JQH22_000755"
     CDS             complement(34967..35701)
                     /gene="pxpA"
                     /locus_tag="JQH22_000755"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFB2205177.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35691..36623)
                     /gene="pxpC"
                     /locus_tag="JQH22_000756"
                     /pseudo
     CDS             complement(35691..36623)
                     /gene="pxpC"
                     /locus_tag="JQH22_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36617..37273)
                     /gene="pxpB"
                     /locus_tag="JQH22_000757"
     CDS             complement(36617..37273)
                     /gene="pxpB"
                     /locus_tag="JQH22_000757"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFB2205178.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37296..38039)
                     /gene="ybgI"
                     /locus_tag="JQH22_000758"
     CDS             complement(37296..38039)
                     /gene="ybgI"
                     /locus_tag="JQH22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFB2205179.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38310..39791
                     /gene="dtpD"
                     /locus_tag="JQH22_000759"
     CDS             38310..39791
                     /gene="dtpD"
                     /locus_tag="JQH22_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFB2205180.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39941..41359)
                     /gene="phrB"
                     /locus_tag="JQH22_000760"
     CDS             complement(39941..41359)
                     /gene="phrB"
                     /locus_tag="JQH22_000760"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFB2205181.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41356..41865)
                     /locus_tag="JQH22_000761"
     CDS             complement(41356..41865)
                     /locus_tag="JQH22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFB2205182.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41994..42281
                     /locus_tag="JQH22_000762"
     CDS             41994..42281
                     /locus_tag="JQH22_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HFB2205183.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42336..42716)
                     /locus_tag="JQH22_000763"
     CDS             complement(42336..42716)
                     /locus_tag="JQH22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205184.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42719..47383)
                     /locus_tag="JQH22_000764"
                     /pseudo
     CDS             complement(42719..47383)
                     /locus_tag="JQH22_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(47402..47605)
                     /locus_tag="JQH22_000765"
                     /pseudo
     CDS             complement(47402..47605)
                     /locus_tag="JQH22_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11404 a        13167 c        11641 g        11766 t
ORIGIN      
        1 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
       61 gctgagctaa tcacccgata ctgcgctgga tactacaaac tacttccacc gaacccaccg
      121 taattagtgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc
      181 tctaccaact gagctaacga cccggtggtg ggtgatgacg ggatcgaacc gccgaccccc
      241 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgacactacg ctggatacta
      301 caactaacta tgttagtggt gggtcgtgca ggatgactcg acttcgtctc gcccttcggc
      361 gccgttgctg atgcaacgtt atccttcacg ttttactacc gcttccacct tagcgattgg
      421 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
      481 gagctaacga cccacttttt cgttgctttc ggtttgtttg atatcccgtg gcaacggcgg
      541 catatattac tgatttcaga cttgagcgca acaaaaattt cgatgcagat cactcaactg
      601 cttatgattc gcacgacacg accagaaata atgcgacttc tggtcgtgtg ttatgcatta
      661 catcgcgttc agacgttttt gtgcctgttt agcgccatca gtaccagggt atttactgat
      721 aacctgctgg tatacggctt tcgcttttgc ggtgtcacct ttgtcctgca tgatgacgcc
      781 gactttaaac atcgcatcag cagcctttgg tgacttcgga tagtttttca ctaccgaagc
      841 aaaatagtac gccgcatcat cttttttacc cttgttgtag tttaactgac ccagccaata
      901 attggcgttt ggcaggtaag ttgaatcagg gtaatttttg atgaaattct gaaatgccac
      961 cattgcgtca tcctggcggg atttatcctg caccagcgca atagctgcat tgtaatccgt
     1021 gtttgcatcg ccgcttttca ccggcgcgcc agcattcgca gtaccagcat cagctgtcgg
     1081 cgtcgttgat gccgtcgcac cgctttgatc gctgctggtt gattgcgccg ctgcaccacc
     1141 gctgctgagg ctgtcgatct gcaacaggat ctgcttttgc cgctccacga cctgattcag
     1201 ttgatactga ttttcctgaa tctgaccacg cagggaatca atatcggatt gattatcaga
     1261 aagttgttgc tggagttggg ttaaaagctg gctgtgagca ttagaaatac gctcaagttg
     1321 agtgacgcgg tcttcgaccg agcctgagcc gacactactg attggtgcct gagcaaaagc
     1381 ggcccagggg gccgctatac caaccagtaa cgacagactc aatagttgat gtctgaagtt
     1441 actgctcatg caattctctt agtaaaccag taccgcacga cggtttttgg agtatgccgc
     1501 ttcgtcatga cccagtactg caggtttttc tttaccgtaa gaaacgatgg agatctggtc
     1561 tgcagaaacg cctttaccct gcaggtacat cttaacggcg ttcgcacgac gttcacccag
     1621 ggagatgttg tattccggag taccacgttc gtccgcgtga ccttctacgg tgactttgta
     1681 agacgggttg ctacgcagga agtttgcatg tgcatccagc atttgagcga agtcagaacg
     1741 gatatcgtac ttgtccagat cgaagtaaac gatgttgttc tgctgcagct gttgcatttg
     1801 cagacgagcc tgctcttcgg aagacatgtt gccgttgccg ccgttcgcat ccataccagt
     1861 gccggcaccc agcatgcctt cgctgccgtc attgctggcg ttcttgttgg aagaacatgc
     1921 cgcaattgcc ataacaggca gagcaatcat cagccctttc agcactttgt tcagttgcat
     1981 ttcaatgatt cctttactat tcaattaatt attatcacag atacggcgac caggcaggga
     2041 atttgacctg tccatcagtt gccggaagac gcgctttgaa acgcccatct gtagaaacca
     2101 aattcagcac ggatcccatc ccctgagaag agctgtagat taccatagtg ccgttaggtg
     2161 ccagacttgg cgtttcatcc aggaacgtgg acgacagaac ttgtacgcct cccgttgcca
     2221 gatcttgttt ggcaatgtgc tgctgcccac cattggagct gaccattacc ataaatttac
     2281 cgtcgctgct gacatccgca tcctggttct gcgaaccttc ccaggtaata cgttgtggcg
     2341 caccgccgtt gatattcact ttataaacct gcggacgacc ggcctggtca gaagtaaatg
     2401 ccaggttctg gctatccggg aaccaggtcg gttcggtatt gttactgcga ccatcagtca
     2461 cctggcggat ctgaccagaa gccaaatcca ttacgtacag gttcagacta ccggttttcg
     2521 acaaggcgaa tgccagtttg ctgccgtctg gcgagaatgc aggtgcaccg ttgtgacgcg
     2581 ggaatgaagc cacctgacgt acagcgccat ttgccagcgt ctgaataacc agcgcggaac
     2641 gaccgctttc gaaggtcaca taagccagtt tagaaccgtc tggtgaccac gccggagaca
     2701 tcagcggctg cggtgaacgg tgaacgacaa actggttgta accgtcatag tcagatacgc
     2761 gcagttcata cgggaactga ccgccgttgg tctgaacaac gtaggcaata cgggtacgga
     2821 acgcaccttt aatgccggtc agtttttcaa acacttcatc actggcggta tgaccagcat
     2881 aacgcagcca ctgcttgttc actttgtacg agttctgagc aagtacagta cccggtgcgc
     2941 cgccagtgtc aacaagttga taagcaacat tgtaagaacc atccggattc ggagtgacct
     3001 gaccgacaac tacagcgtca atgcccagtg cggaccatgc agctggttgt acttcctgcg
     3061 cactacccgg ctgctgtggc agacgagcgc gatctaacgg attaaattta ccgctgttac
     3121 gcaagtcagc agcaacgatg ccgccaatat cttcaggtgc cgcaccaggc cccgcccact
     3181 ggaaaggaac aacaccaata ggacgaccgg aatctacacc gctgtcgatc acaatgcgga
     3241 cttcagcatg cagaactgat gcccacagta tgagaaaacc aaatgctact cgtaatgcct
     3301 gcttcatcat atctccctta tctggaccga tggcccacga taatttagca gaatgttaac
     3361 aaactaaaat acacaaaact accagaaccc cgtggcaacc ggtgcctgat gttgaccgtc
     3421 cgaacagtca acatcgcgat tacggtttga agtccaatgg cgcgtttttg aacacttcat
     3481 atactgcctg gcttggtggt ttcgggatct tcgcaagttt agctgctgcc aacgcagcct
     3541 gacaaagtgc gggatcgcca ccttcaggtt tgatatccag taacatacca tcgggtgcca
     3601 gttttatgcg cagcgtacag gttttgcctg cataggacga tgcgtcatag aacttacttt
     3661 cgatagcaga tttaatctgc ccggcatagt tattgatatc ggcccctgat gcgccattgt
     3721 ttttagtatt accactcccg gcaggcgaag cattgttccc tttcgcccct ccccccgttt
     3781 tcggtgcatt cttaccagag cttagctcac cgaaaatatc atctgcctct gcggcagctt
     3841 ttgctgcagc ggctttttca gctgctgctt ttgccgctgc cgcttttttg tctgcggcgg
     3901 ctttttctgc cgctgctttc ttatcagctg ctgccttatc agcagccgct ttttctgctg
     3961 cttttttgtc ggctgcagct ttctctgctg ccgctttctt atcagctgca gccttttcag
     4021 cagccgcttt cttctcagct tctgccttgg ctttttcagc agcttcagtt gctgctttct
     4081 ttcttgcttc agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg
     4141 ctacctcggc ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt
     4201 tcttcgcgtc tgcagccgct ttcttcgctg cttcttccgc agctttagca tctgccgccg
     4261 ctttcgctgc cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt
     4321 cagcctgctt tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt
     4381 cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc
     4441 gctgttcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa
     4501 ctaccgcacc tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg
     4561 aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca
     4621 gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca
     4681 ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca
     4741 gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag
     4801 ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc
     4861 gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa
     4921 cgctccaggc gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca
     4981 atcactggcg gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg
     5041 acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc
     5101 agtacgtcca gcaacggaac aatgttgatt tcggacttga gatcgcgacg acctcgtcca
     5161 cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc
     5221 agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc
     5281 tggttgagac ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc
     5341 gcaatcaacg cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc
     5401 ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg
     5461 ctgatggagc caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg
     5521 ttcatggaga tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta
     5581 ttggcacgat gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca
     5641 gtcagattat cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac
     5701 cagaatttat cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc
     5761 tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta
     5821 accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact
     5881 ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa
     5941 ccagaacctc tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa
     6001 ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc
     6061 gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca
     6121 tcagcgcctg ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa
     6181 aagcgacgta actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc
     6241 gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta
     6301 gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta
     6361 agggcgcgta aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat
     6421 ttaaaagaag aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa
     6481 aatcccttgc caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca
     6541 gacggcccac atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa
     6601 acgagatggg tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc
     6661 ccgcaggggg cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg
     6721 tcttcttgac cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca
     6781 aacgaacagg caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta
     6841 cttagtacag agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt
     6901 tccagtaaca ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg
     6961 tcatgacgtt aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca
     7021 tcatggtgct ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc
     7081 aggccagcgt cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca
     7141 ggatggtcag cagcagcaga accacaccca gtgccggaat agcccacaga attggcgtgt
     7201 tgttgaagtt aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag
     7261 aggctgcgta atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc
     7321 atacgccagc cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac
     7381 gggtacgcag gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa
     7441 tgatcatccc tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga
     7501 agttaccggt gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca
     7561 ggttaccgaa cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc
     7621 agtcccacat gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg
     7681 gacggaagaa caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag
     7741 cggcatagac catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct
     7801 ggttaccgtc ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac
     7861 gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac
     7921 caatcagcag aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca
     7981 tttgacgact cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat
     8041 agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt
     8101 ctgccaccag gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc
     8161 ctgcggtcag tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt
     8221 ggcggccata ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac
     8281 cgtacagcgc ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc
     8341 agaaagagag cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg
     8401 caaagtacag cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt
     8461 cagtcgcatc agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt
     8521 tcttcatgct attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt
     8581 cgagcagaga gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct
     8641 ctttcaggcc gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag
     8701 ggatctgaat cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa
     8761 aggcagcagg cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct
     8821 gcacgtcgcc catttcgtag ccggattcat cacccagaac aataacagac agaacagcag
     8881 ccataccgaa gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt
     8941 tcagcatata ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg
     9001 ctacagtgtg aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca
     9061 ccatctccat acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc
     9121 cgttcgcaac cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga
     9181 cacacatatg ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg
     9241 tggattcgag gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc
     9301 ctacatagtg ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg
     9361 tagccacacc cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt
     9421 tataaatctg tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca
     9481 taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc
     9541 gcgacagttc gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga
     9601 gtgaaccccg ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc
     9661 atcataccat gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa
     9721 aaactcaaat tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt
     9781 tgttgaagca aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc
     9841 agccaatttg atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat
     9901 tgataaatat caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga
     9961 tatttatgtt taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag
    10021 ataaaaagag acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc
    10081 taatacgacg cagagtttca ggattattaa taaaggcata aatctccccc ccgccgatat
    10141 taacatttaa ttaacttata attatattat aattaactca cccgtggtta ttattttgtt
    10201 aaaaataaag ccacatatac gtagcttgag agtgaagata aacacaaagt tacctgatgc
    10261 gctacgcttg ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag
    10321 accgaataaa gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg
    10381 gccgcgcata tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg
    10441 tgactggccg ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat
    10501 gtgttcatcc atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc
    10561 atcacaggtt gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc
    10621 agctttctta agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact
    10681 ttccggcacg ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta
    10741 acactgtact ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt
    10801 accggtataa ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg
    10861 gactggcatt ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag
    10921 taagcccacg ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc
    10981 aaattcacgt agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc
    11041 atagttgagc atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt
    11101 gttcattgca ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac
    11161 actatcggtg ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt
    11221 atcaaggttg atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc
    11281 gacatctact ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc
    11341 catttcatag cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg
    11401 tttcgcattc gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc
    11461 accatcatca gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt
    11521 atctaccagt tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag
    11581 tatcccttca gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata
    11641 aagcgtgcga tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa
    11701 gggatcgtaa ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg
    11761 ggcatggcga ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct
    11821 ggcgcgcagc cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa
    11881 caggacgagt ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa
    11941 acgcagcaga ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg
    12001 aactttgtca ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt
    12061 ccacattttc tccagcaagc cgtggtggta tccaaaaccc aacgtcctgg ccagcgttgc
    12121 cagcggttcc agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc
    12181 catgcgcgtg gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc
    12241 tttcagggtt gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg
    12301 gctcatcaca aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat
    12361 attttgctgc aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga
    12421 cgctttttcc agcacgtcaa aataactgtc gaggcgtttg cgtaatccgt tttcgtccgc
    12481 aggtaagtac ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc
    12541 gtcactgctt tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg
    12601 ccagaacatg gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat
    12661 gccaaaggaa tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg
    12721 cattccgtac atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg
    12781 ggtatactag gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt
    12841 gttttccggt ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta
    12901 atatttgtat tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag
    12961 cagaatgcgt gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt
    13021 gatgtgaacg cgagataccg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca
    13081 tcagtcagat agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc
    13141 gagattgcag cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc
    13201 gcgccattat catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg
    13261 atattcatcg caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata
    13321 acccgcagcg gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct
    13381 aacagccagg tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc
    13441 atcgtggaag cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac
    13501 gggccgtaaa cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact
    13561 ggccctccaa ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta
    13621 cttcctgaca gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc
    13681 cccaccacaa acagcatcag actgcccgct cccaatgtac cgagcaccgg gtagagataa
    13741 aaagtcagga agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc
    13801 aagtaaccgg ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg
    13861 gcggcaagtc cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg
    13921 gtataggctg ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg
    13981 tacatccaca gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac
    14041 agtaataccg cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggcaaaagag
    14101 atcccgctca acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct
    14161 tgttgtaagg tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct
    14221 tctgcatggc gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc
    14281 tcactctcct tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga
    14341 tccgccgtta aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg
    14401 ccgagtttgc gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc
    14461 gccgggcagg ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc
    14521 gtgcctccct tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata
    14581 cgcgcccgaa tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc
    14641 gtagtacccg cttcattgag gggaatcgcc agcaacacca ctaaatcaac cgcttccggg
    14701 ccatccacgc cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct
    14761 tttaccgcag cagttttgcc atgcggcaca gccaaccctt cacttaaggc ggtcgggcca
    14821 aggctttcac ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga
    14881 gcagcaagac gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc
    14941 agacacaacg catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg
    15001 tttttatagt cgcgccagtg tgcccgacaa aacaatacca ttgcgtaatc aatttcactt
    15061 gattgaaatt atttgcattt tatttgtatt gtttacagga tgaatttatc gggcataatg
    15121 gctaaattcg tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt
    15181 gctgatcgca ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct
    15241 accgaatcgg cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg
    15301 cgccagttag tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa
    15361 gaagagcggg tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac
    15421 cgtcacgtcg atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt
    15481 cttcagcaac agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc
    15541 tatcgcaagc aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg
    15601 gatctcacct ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag
    15661 atggttatcg atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc
    15721 cgtctgctta atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta
    15781 gttgatggtc gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt
    15841 accctgatag cgcagcgaaa atcatcgcga taagcacaaa aaggccatca tacgatggcc
    15901 aaccatgtcg aaaccgacag cccttcgcca ttcctgtaga gggaagcggc gagggctatt
    15961 tcttattaca gatatttatt tcagaacagt tttcagtgct tcaccgatat ccgccaggct
    16021 gcgaacggtt ttcacgcctg cggcttccag agcagcgaat ttctcatccg cagtcccttt
    16081 cccaccggca atgatcgcac ccgcgtggcc catacgtttg cctttcggcg cagtcacacc
    16141 agcgatgtaa cccacaactg gcttggtaac atgctctttg atgtacgctg ccgcttcttc
    16201 ttcagcgcta ccgccgatct caccgatcat cacgatcgct tcggtctgcg gatctttttc
    16261 gaacatttcg agaatgtcga taaagttaga gccagggatc gggtcaccgc cgataccgac
    16321 acaggtcgac tgaccgaaac cgtaatccgt ggtctgttta accgcttcat aggtcagtgt
    16381 accggaacgg gaaacgatac ccactttacc cggtttgtga atgtgacccg gctggatacc
    16441 aattttgcat tcacccgggg tgataacgcc tgggcagttc gggccgatca tacgaacgcc
    16501 tgcttcgtcc agtttcactt tcacggtcag catatccagc gtcgggatgc cttcagtgat
    16561 ggtgataatc agtttgatgc ctgcatcgat agcttccaga atggagtctt tgcagaacgg
    16621 tgctggtacg taaataacag acgcggaagc gccagtagca gcaacagctt cacgcacggt
    16681 gttgaacacc ggcaggccga ggtgggtagt gccgccttta cctggagtta cgccaccaac
    16741 cattttagtg ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc taccggtaaa
    16801 gccctggcag ataaccttgg tgtttttatc aattaaaatg gacattattt cccctccact
    16861 gcggcaacaa cctgctgagc tgcatccgtc agaccttttg ctgcaataat attcaggccg
    16921 ctgtcagcca gtttcttcgc gccgagttcg gcgttgttac cttccagacg taccacgacc
    16981 ggtacgttaa cacccacttc cgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa
    17041 cgaacgatac cgccgaagat gttaaccaga acggctttca ctttgtcgtc agagaggatg
    17101 attttgaacg cttcggttac acgttctttg gttgcgccgc cgccaacgtc caggaagtta
    17161 gccggttcgc cgccgtgcag tttaactatg tccatcgtac ccatcgccag acctgcgccg
    17221 ttaaccatac aaccgatgtt accgtccagc gcaacgtagt tcagttccca ctgtgcagcc
    17281 tgtgcttcac gcggatcttc ctgcgactgg tcacgcattt cgcgcagatc aggctggcgg
    17341 aacagggcgt tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg
    17401 gtgatgacca gcgggttgat ttcgatcagc gccaggtcgc gctccaggaa aatggtcgcc
    17461 aggcccatga agattttggt gaactgctga accagtttac cttccagacc cagtttgaac
    17521 gccagctcgc gtccctgata cggcatcggg ccagtcagcg gatcaagcgc aactttatgg
    17581 atcaggtgcg gagtttcttc cgccactttt tcgatttcca cgccgccttc ggtggaggcc
    17641 ataaagacca cgcgacggga actacggtct acaacggcac cgagatacag ctctttagcg
    17701 atatcggtcg ccgcttcaac cagaatctgg ttaaccggtt ggccattggc atctgtttga
    17761 tacgttacca gacgcttgcc cagccagttt tctgcaaaag cacggatgtc ttctttgctg
    17821 tttacaactt tcacaccgcc cgctttaccg cggccaccag cgtgaacctg acatttcact
    17881 acccacggac cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg agtagtacag
    17941 gcataaccca ccggtgctgg taagccatag cgggcaaaca gttgttttgc ctgatattca
    18001 tgtaagttca tgtgttctgt ccatccttca gtaatcgtta tcttttaaac cgtagaccga
    18061 caccctatcc acgtcggtct acagtgcagg tgaagcttaa actactacac gtccagcagc
    18121 agacgcgtcg gctcttccag taactctttg atcgttacca ggaagcccac ggattcgcga
    18181 ccatcgatca gacggtgatc gtaggacagc gccaggtaca tcatcggcag gatctcaacc
    18241 tgaccattca ccgccatcgg acgatctttg atagcgtgca tacccagaat tgcgctctgc
    18301 ggcgggttga tgatcggcgt agacatcagg gaaccgaaca caccaccgtt ggtgatggtg
    18361 aagttaccac cggtcagatc ttcaaccgtc agcttgccat cacggccttt gactgccagc
    18421 tctttgattt tcttctcgat gtctgccatg ccgagggtat cgacatcacg cagaaccggc
    18481 gtcaccaggc cgcgcggcgt agaaaccgcc atgctgacgt cgaaatagtt gtggtaaacc
    18541 acgtcatcgc cgtcgataga cgcgttcact tccgggtaac gtttcagggc ttcaaccacc
    18601 gctttcacgt agaaggacat aaagcccaga cggatgccgt ggcgtttttc aaacgcttca
    18661 ccgtactgct tacgcagatc cataatcggc ttcatgttga cttcgttgaa cgtggtcagc
    18721 atggcggtgg agtttttcgc ttccagcaga cgctctgcca cacgcttacg caggcgagtc
    18781 atcgggacac gtttttcact acgtgcagcc agagccggtt gcgccgccgg agcagccgct
    18841 gccggagcag actctttcgc cggggctttc gccagatgtt tttccacatc ttcacgagtc
    18901 agacgaccac ccacaccggt gcctttaatg gcgctggcgt cgagattgtg ttcagccagc
    18961 aggcgacgga tcgccgggct taacgcatcg ttgttttgct cttccagaga cgcctgctgg
    19021 cgttgcgccg gagtggacgc tttctcttca gatttggcgc tggtttcttt accggcgctg
    19081 ttgccttcac gcaggcgacc aaggatctga cgagacgtta ccgttgtacc ttcatcttcc
    19141 agaaccgcat ccagaatgcc gtctgctgat gccggtactt ccagtaccac tttgtcagtt
    19201 tcgatttcta ccagcacttc atcacgtacg actgcgtcgc cgggtttttt atgccaggtt
    19261 gcgacggtgg catcggctac ggattcaggc aggtcaggga ccagaatatc tacgctactc
    19321 attgtgtatc ctttatttat tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt
    19381 tctggtgaac ggacatatac cctaccgccg gagaggcgga ggccgggcgg cctgcataac
    19441 gcagagaagc cccaaacgga atcacttcac ggaaatgatg ctggctgcag taccatgcgc
    19501 cctggttgag cggctcttcc tggcaccaga caaaatcctt gacgtgagca aactgctgca
    19561 acacttcctg catcgcttta tgcgggaacg ggtagagttg ctcgatacgc acaatggcga
    19621 catcgtgttg attgttctta cgacgctgtt ccagcaggtc gtaataaacc ttaccagaac
    19681 acatcactac gcgcttcacg cccttcggat caagctcgtc gatttcaccg atggctggca
    19741 ggaaggtgcc gttcgccagt tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg
    19801 atttcggcga catcacgacc agcggacgac gcatcccgcg cagcgcctga cgacgcagca
    19861 tgtggtaaac ctgtgccggg gtagacggta cgcaaacctg catgttttgc tcagcacaaa
    19921 gttgcagata acgttccaga cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt
    19981 gcggcagcaa catcaccaga ccacacatcc ggccccattt ctgctcgcca gaggagatga
    20041 actggtcgat aaccacctgc gcaccgttgg cgaagtcacc gaactgcgct tcccagatgg
    20101 tcagggtgcg tggttctgcg gtggcataac catattcaaa cgccagcacg gcttcttctg
    20161 acagtacgga gtcccagaca cggaacgcgc cctgaccgtt atggatgtgt tgcagcggcg
    20221 tgtaagtgga accgttagac tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc
    20281 gaccggagtc ttcacccgac aggcgaaccg gaatgccttc atcaaccagc gtggcgtaag
    20341 cgaggttttc cgcaccgccc cagtcgaaca gtttctcacc ggctgccatc gcctggcgat
    20401 cgccataaat cttggcaacg cgagactgca tttcaactgc ttctggtacc gtactgatac
    20461 gtttagccag ttcctgcagg cgcttcatct caactttgtt cgggtactct tcgtcccatt
    20521 cgtggttgag gtacggcgac caggtgaaag agtgcatgtt catcggacgc cactctgcca
    20581 ctacgcaatc gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt
    20641 ccagcgtcgc cactttttcc tgctccagct tgtcagcgta gattttgcgc ggtgtcggat
    20701 gttttttgat tttctgatac atcagtggct gggttgcgct cggctcgtcg gcttcgttgt
    20761 ggccgtgacg gcggtagcat accaggtcga tgaagacatc acgtttaaag gtgttacgga
    20821 aatcgagcgc cagacgggtc acaaaggcaa cggcttccgg atcatccgcg ttaacgtgga
    20881 aaatcggtgc ctgaaccatc ttaccgatat cagtacagta cggtgtagaa cgggcatcca
    20941 gcgggttaga ggtggtgaaa ccaacctggt tgttgataac gatacgtacc gtaccgccaa
    21001 cttcataacc acgcgctttc gacatgttca gagtttcctg aaccacgccc tgcccggtca
    21061 ctgcggcgtc accgtggatg gtgattggca gcactttgtt gctgttcggc tcatcaagtc
    21121 tgtccagacg ggcacgaaca gagccgataa cgaccgggct tacaatctca aggtgagacg
    21181 ggttaaacgc cagcgccagg tgcaccaggc cgccatcggt ctggaagtca gacgagaagc
    21241 ccatgtggta tttcacgtcg cctgtgccga ggtgttcttt atgtttaccg gcgaactcgt
    21301 cgaacaagtc ttgcggtttt ttacccagca cgttcaccag cacgttcaga cgaccacggt
    21361 gcgccatccc gagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt
    21421 taagcatcgg gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt
    21481 ttgcgccgag gtaacgctca aggccttcag cggcggtcag ttcgcttaag aagcgttttt
    21541 tctcttcgct attgaaagtc gcgcgaccag actcgatacg ctgttggatc cagcgttttt
    21601 cttcggtgct ggtgatgtgc atatactcgg caccaatcgg gccgcagtag gtttgcttga
    21661 gggcggaaat aagctcgccg agtttcatgg tttctttgcc gctggcaaat gaaccgacgt
    21721 tgaaggtttc ctggaagtct gcttcggtca gatcgtggaa agacggatcc agatcggcca
    21781 ctttatcttg ctgccacagt cccagcggat ctagattcgc atgctggtga ccacggaagc
    21841 ggtatgcgtt aatgagctgc aggactttaa cctgcttcac attggtgtca gggtcggaga
    21901 tcgttgaaga gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag
    21961 agtggaattg atccggtttg actcccgtac caggtaactg ctggaacgtc gaacgccagt
    22021 tagcgtcaac cgagtcagga tcggttaaga agtcttcata gagctgttct atccagctct
    22081 ggtttgcgcc agagaggtaa gaagagtcca accaggcttt caaagcgctg ttctgcatcg
    22141 tgatccctta agcatctttt ttatgcttac ttcgccgtgg atactaccac gcacagtgat
    22201 gtgcgtgccg gggttcactt gcgagctctt gtctacgtag tggctcgcga aggaaccttt
    22261 aaaaactgtc ttagtgtcag gggcagtttt taaagatttc ctgcatttct ttgttacgcc
    22321 tgatgcgctg cgcttatcag gcctgcgggt aatgacttgt aggccggata aggcgttcac
    22381 gccgcatccg gcactggttg cctgatgcaa cgcttgcgcg tcttatcagg cctacagttt
    22441 acgcattacg ttgcaacaac atcgacttga tatggccgat ggcgcgcgtc gggttcagcc
    22501 ccttcggaca tacactgacg cagttcatga tgctgtgaca gcggaatacg ctgaatgcat
    22561 cgctcaaacc gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc aggaaacgat
    22621 atgccgctaa caagcctgcc gggccgataa acttatcggg attccaccag aaagacggac
    22681 aagaggttga acaacatgcg cagagaatac attcatacag cccgtcgagt ttttcgcgct
    22741 gctctggcat ctgtaaatgc tcgcgagctg gcggattttg tccattattc aacaggtaag
    22801 gcttaatttt ctcatattgc gcatagaatt gtcccatgtc taccaccaaa tcgcggatca
    22861 ccggtaaacc tggcagcggg cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa
    22921 tcggggtaat acaggccaga ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca
    22981 caccttcacg gcaggagcgg cggaacgaca ggctgggatc tttctctttc agctggataa
    23041 gcgcatccag cagcatcatg tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac
    23101 gcggagcatc atcaacatcc gggttatagc gataaattga aaactcgagt ctcattttcc
    23161 tgtctccgca ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca
    23221 tgttgacgct tcggcgcgtc atggattccg actcaggcag atacagggag tggcacagcc
    23281 agttttcatc atcacgatcc gggaagtcga agcggctatg tgcgccacgg ctttcggtac
    23341 ggaagttggc agaaacagcc gttgcatacg ccgtttccat caggttatcc agttccaggc
    23401 actcaacgcg ctgggtgttg aactcgctgg aagtgtcatc cagacgggca tttttcagac
    23461 gctcgcggat cactttcaac tgctcaagcc ctttcgccat cgcatcacct tcacggaaga
    23521 ccgagaagtt atgctgcata cattcttgca gcgctttacg gatcgccacc ggatcttcac
    23581 cgttacgatt attgttccac cggttcaggc gatccagaga cgcttcaaca tcagactcgc
    23641 tggcatcgtg cagtgcgccc tgctcggcga tagactcttg cagatgcaga cctgccgcgc
    23701 gaccaaagac caccaggtcc agcagcgagt tgccgcccag acggttagcg ccgtgtaccg
    23761 atacacaagc gatttcacca acggcaaaca gtcccggaac aaccacatct tcgcctttct
    23821 cattcacagt cagcgcctga ccggtaactt tggtcggaat accgcccatc atgtagtgac
    23881 aggttgggat aaccggaatc ggctctttca ccggatcgac gtgagcgaag gtacgggaaa
    23941 gctccaggat acccggcaga cgggattcga gaacttcttt acccaggtga tcgagtttca
    24001 gtttcgcgtg tggcccccac ggaccatcac agccgcgacc ttcacggatt tcaatcatga
    24061 tggaacgcgc caccacatca cggcccgcca ggtctttggc gttcggcgca taacgctcca
    24121 taaagcgttc gccatgtttg ttcagcagat aaccgccttc accacggcaa ccttcggtga
    24181 ccagtacgcc cgcaccggca atgccggtcg ggtggaattg ccacatttcc atatcctgca
    24241 ccggtacgcc tgcgcggata gccatgccga caccgtcgcc agtgttaatg tgggcgttag
    24301 tggtggactg ataaatacgc cctgctccgc cagtcgccag cacggtagcg cgggctttga
    24361 aataaaccac ttcaccggtt tcgatgcaca gtgcggtaca acccaccacc gcgccatcct
    24421 ggtttttcac cagatccagc gcataccact cggagaaaat ggtggtgtgg tttttcaggt
    24481 tctgctgata aagcgtgtgc aacagtgcgt gaccggtacg gtcagcagcc gccgcagtgc
    24541 gtgccgcctg ctcgccgccg aagtttttcg actgaccgcc aaacggacgt tgatagatac
    24601 gaccatcatc gagacgcgag aacggcaggc ccatgtgttc gagttccaga atcgcttccg
    24661 gcccggtttt acacatatat tcaatcgcgt cctggtcacc gatatagtcc gaccctttta
    24721 cggtgtcgta catatgccat tcccagttat cttcatgggt attacccagc gcaacggtaa
    24781 tgccgccttg cgcagaaacg gtatgggaac gggtcgggaa gactttagag agcagggcac
    24841 aggtctggcc gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa
    24901 tcacaactgc atcaaattct ctgactggca atttcatcac acaccccaca ccacaacgaa
    24961 tccataaatc acgtaaacca ccagtgcaac gacaatcacc agttgcagca tcaggcgcaa
    25021 ggccagcggt ttaacgtagt cggtcaacac ctgccacatg ccgatccagg catgaatcaa
    25081 gatagaaaac agcgccagca gggtgaacac tttggtgaac gcagaggcga agaaaccgat
    25141 ccagacttca tatgtcagtt cgccactggt ggcgaaaaaa ccgaccatat aaatgatgta
    25201 gagcgtcagg acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc
    25261 taatgcggag gcgttgctta ccatacgagg actcctgcga gaagtgaaag cacgacagta
    25321 ataacaaagg agattttggc ggagcgttta cccgcttcga atgtttcttc cagatagcca
    25381 aaatccatca tcatgtggcg aatacctacg acgacgtgat acgccagagc ggtaaggatg
    25441 ccccacatga taaatttgac gaagaagctg cccataatcg cggaagcttg ctcgaaacct
    25501 tcaggggaag agaggctggt acccagaagc cactgcagga tgcccactgc aacaaaggtg
    25561 atcacaccgg aaacgcgatg gagaatggac gctatcgccg tgatggggaa ccggatggtc
    25621 tgtaggtcca gattaacagg tctttgtttt ttcacatttc ttatcatgaa taacgcccac
    25681 atgctgttct tattattccc tggggactac gggcacagag gttaactttc tgttacctgg
    25741 agacgtcggg atttccttcc tccggtctgc ttgcgggtca gacagcgtcc tttccataac
    25801 tgcgcgtcat gcaaaacact gcttccagat gcgaaaacga cacgttacaa cgctgggtgg
    25861 ctcgggattg cagggtgttc cggagacctg gcggcagtat aggctgttca caaaatcatt
    25921 acaattaacc tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata
    25981 acaacattta tttaattttt aatcatctaa tttgacaatc attcaacaaa gttgttacaa
    26041 acattaccag gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat
    26101 taacttatgt aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtag
    26161 acaagtttaa taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga
    26221 ttctttacct gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg
    26281 cgagccaaat aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg
    26341 cagtcttacg caataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca
    26401 acctcaacgg ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg
    26461 ttattgatat ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt
    26521 caaccgcatc ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc
    26581 accgcggttt cccgatcgat cagctggcga ccgattctaa ctacctagaa gtttgttaca
    26641 tcctgctgaa tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga
    26701 cccgtcatac catgatccac gagcagatta cccgtctgtt ccacgctttc cgtcgcgact
    26761 cacatccaat ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact
    26821 cgctggatgt taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa
    26881 tgccgaccat ggccgcgatg tgttacaagt attccattgg tcagccattt gtttatccgc
    26941 gcaacgatct ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac
    27001 cgtatgaagt taatccgatt ctggaacgtg ctatggaccg tattctgatc ctgcacgctg
    27061 accatgaaca gaacgcctct acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc
    27121 cgtttgcctg tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta
    27181 acgaagcggc gctgaaaatg ctggaagaaa ttagctccgt taaacacatt ccggaatttg
    27241 ttcgtcgtgc gaaagataaa aatgattctt tccgcctgat gggcttcggt caccgcgtgt
    27301 acaaaaatta cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag
    27361 agctgggcac caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga
    27421 acgacccgta ctttatcgag aagaaactgt acccgaacgt cgatttctac tctggtatca
    27481 tcctgaaagc gatgggtatt ccgtcttcca tgttcaccgt cattttcgca atggcacgta
    27541 ccgttggctg gatcgcccac tggagcgaaa tgcacagtta cggtatgaag attgcccgtc
    27601 cgcgtcagct gtatacagga tatgaaaaac gcgactttaa aagcgatatc aagcgttaat
    27661 ggttgattgc taagttgtaa atattttaac ccgccgttca aatggcgggt taatttttat
    27721 atgcctaaac acaaaaaatt gtaaaaataa aatccactat cggacattca tatattctaa
    27781 aaaaaaatac atcggtataa attataaaca aatagatgat ttttaatcat ctatcttcct
    27841 ctcagttaca tgaataaacc accaaatata gcgcacccct ataattccca atactttatt
    27901 catcaaacat ttaattcata ccatcaatta attactttta catttaataa aattaataat
    27961 atttttaaga aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac
    28021 ttttttcttt tctgttaaag gactcagtta tgtttaaagg acaaaaaaca ttggccgcac
    28081 tggccgtatc tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa
    28141 ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata
    28201 aagaggttga actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg
    28261 ccgttgctgt cgacctgctc ttagtcaact gtgatctgga aaactccagc aacggttccg
    28321 gtggcaagat ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag
    28381 atccgattct caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga
    28441 ataaagatca aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga
    28501 cctccagcga acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac
    28561 ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat
    28621 tactttggcg gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acaccgtgaa
    28681 tatttatcga ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt
    28741 cgaattcaat ctgaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt
    28801 aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa
    28861 ccaaatcagt aacgagcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg
    28921 catcaatgat ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc
    28981 attgataaat cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca
    29041 agccaatcag caactgaata ttagtattcc gcaagcctgg ctggcgtggc actcagaaaa
    29101 ctggacccca ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt
    29161 gtttgccagc agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg
    29221 aacagccgga atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcatac
    29281 tgatagcgat gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc
    29341 gttaccgcaa ttaggttcaa aattaaccct cggcgaaacg gattttagtc ccaatatttt
    29401 cgacggtttt tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga
    29461 actacgcggc tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat
    29521 cagtcaatca ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga
    29581 cgaccttaat cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg
    29641 ggtgaacaat ttccaggttt cagcagcatc gacgcccttc ctgactcgtc agggacaggt
    29701 tcgctataaa ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga
    29761 aacctttttt agcaatgaag tttcctgggg gatgctgtca aacacctcgc tgtacagcgg
    29821 cctgctgctt tctggtgatg actaccattc tgccgcaatg ggtattgggc aaaatatgct
    29881 gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt agccattttg atactcagca
    29941 ggacgagcgg ggcttaagct accgttttaa ttacagcaaa caagtggatg ccactaacag
    30001 cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta
    30061 tctggatcac aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc
    30121 tgtgggccaa ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac
    30181 ctggtggaat gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat
    30241 tggtgactgg agagatatct ctatttctac gtcattcaat acaacccatt acgaagataa
    30301 agatcgcgac aaccagattt acctgtcgat ttcgctcccc ttcggtaacg gtggtcgggt
    30361 tggctatgac atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac
    30421 gctggatgaa cgtaatagct ggggcatgtc tgccggactg caatccgacc gtcctgacaa
    30481 tggagcccag gtgagcggta actatcagca cctgagttca gcgggtgagt gggatatttc
    30541 tggtacctat gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac
    30601 cgcaacccaa tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat
    30661 ggtcagcacc gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca
    30721 ttttggcatt gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa
    30781 catgaatgac ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat
    30841 tgaaggcgcg ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat
    30901 cattcgcaac gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag
    30961 cggcattagc gtggggatgg ttggcgagga aggacatgcc tggttaagcg gagtcgctga
    31021 aaatcaaaag tttaccgtgg tctggggtga tagccagcat tgctcgctcc atcttcctga
    31081 acatatggaa gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa
    31141 atgacattta tgaaaggact accccttttg ttgttggttg ccagtctgtg cagccacgct
    31201 gcactacaac ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg
    31261 cgggtggata atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa
    31321 aaaggggaga aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg
    31381 aaagcaacaa cacaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat
    31441 cgcgaaacgc tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc
    31501 gaccatgccg ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct
    31561 gcattacgca agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccagcagggc
    31621 aaccaactga cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga
    31681 aatgaaaaag gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc
    31741 aacacaaaca ccggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac
    31801 ggcgcgttac gcatgacgac gctcaactgc agcggacaat gccatttaca agccgtggag
    31861 gcgaagaaat gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgtcgt
    31921 tgaagtctgc gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat
    31981 cagaagcgat tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatctggg
    32041 aatctgacga tattaaaata agatctggga atctgacgat attaaaattc ccgtctattg
    32101 tgacaacaat accaacggca actttgaaag cgaacatgtc tacgcatggg taaaccccta
    32161 cccgggggtg caggatcgtt attatcaact cggagtaact tacaatggcg ttgattatga
    32221 cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt atcgacagta agaacattga
    32281 catttatacc cctgatggga tggcagaaca aaatttgctc gggtgatccc gctaatattc
    32341 acatgtcgcg aacctttctc gctcgcatgc ggttatacgt caaaatacga gaaatgccgc
    32401 cgcatgatta tcaaagtacg cttagcgact atatcgtcgt gcaatttgac ggtgccggta
    32461 gcgttaacga agaccctact gcccaaaacc tgaaatatca tattactggt ctggaaaaca
    32521 ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc ggaaacacag atgattgatt
    32581 ttggtaaatt taatttgctg gatattcgtc gccacacaat gtcgaaaaca ttcagtatta
    32641 agacgacaaa atcacaaaat gaccaatgca ccgacggatt taaagtcagt tcctcctttt
    32701 ataccgaaga aacgttggtt gaagaagata aagcattgtt gattggtaat ggtctgaagc
    32761 tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa taaatatgct gaatacgccg
    32821 atttcaccag cgacatgttg gtctacgaaa aaacctatac agctgaactt tcgtccattg
    32881 caggtacccc catcgaggct gggcccttcg atacagtggt gctttttaaa attaactata
    32941 actgatttgc catgatgccc tttgcagccg gaaagggcat ctctttcaga aaattgctga
    33001 ttttcccctt ttccttgatt gctgttattt tttcctagcc cggctttctt tatgctgtat
    33061 cgatacacca aagagaaaat aatgagggag cgaaggatgc cagttttgca gtggggaatg
    33121 ttatgtgtgc tatcacttct cctttctatt ggtttcctcg cggtacatct cccggcggcg
    33181 ctattacttg ggccgatgat cgccgggatc atcttcagta tgcgcggaat tactctgcaa
    33241 cttccccgct ccgcttttct cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac
    33301 ctcactggtt cgattctcac cacgctggcc gccaactggc cgatcgtgct tgctatttta
    33361 ctggtgacgc tgctttccag cgccatcgtg ggttggttat tggtgcgcta tagctcactg
    33421 cctggaaata ccggtgcctg gggttcttct ccaggcggcg cagcggcaat ggtcgctatg
    33481 gcgcaagatt acggcgcaga tattcgcctg gtggcgttta tgcaatatct gcgagtgctg
    33541 ttcgtcgcgg gggccgctgt tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg
    33601 gttaaccagc aaatcgtctg gttcccgcca gtgagcatta atctcctgct tacgattttg
    33661 ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc
    33721 atcccgatgc tggcgggcgc agtgctccag tctggtcagc tcatcaccat cgaactaccg
    33781 gaatggctgc tggcgatggc gtatatggca attggctggc ggattggtct tggtttcgat
    33841 aaacaaatct tactgcgggc actacgcccg ctaccgcaaa tcctgctgtc gatttttgct
    33901 ctgctggcta tttgtgcagg tatggcgtgg ggactgaccc ggtttatgca tattgatttt
    33961 atgactgcct acctcgccac cagtcccggc gggcttgata ccgtggcggt catcgccgca
    34021 gggagcaatg ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt
    34081 ttgctgacgg ggccagccat tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc
    34141 tagtgctggc agcctggaca ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg
    34201 atgccgccac agcgttcgca cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc
    34261 gccccatgat gcttattctc atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc
    34321 tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt cgctttatga
    34381 tttccagtca acccaacctg ccagaggatc tccacccgca aataattgcc aagcccagcc
    34441 agaaacgcct gatcgagcag taatcctgca aactgacggt tacgaaagcg cggcgacaat
    34501 aaccgttcct ttaccacctc cggcgtcaga ttcggatcca gcacatcggg accaacgcgt
    34561 tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta
    34621 taaagcagaa tggttttgtc agccgtttgc agttttaccc gcaatactcg cgtggtctgc
    34681 ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa gctgattatg gctgtagagc
    34741 gttaaaccgt tgggaaaatg agttaacaac gccttaccac gcgtttccac atgggtaacg
    34801 tgttgaccga taagttgtga ttgataagtt tttaactgcg ggaaggcaaa ccagacatca
    34861 gttagtggtt tgcctttgat cgccgcctcc aggttatccg ctgcacggcg gatctccggg
    34921 ccttcaggca tggtgatatc cttttttcat tctttattaa gcggggttat gctgcgacaa
    34981 caatcccctt tttggcaaat gtagctcgta atcggcgggc gaacgccaga gcgtgctcgc
    35041 cgtcgccatg caggcagacc gtttgcgccg cgaccgttgc ccattcgccg gtgatacttt
    35101 tgactctgcc gtgttgcacc atctccagcg tttgcgccaa cgcctgttct tcgttttcaa
    35161 tcaacgcgcc tggctgactt cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg
    35221 caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc agctcgcttc
    35281 ctgccagccc aacgagaacc aatgccggat cgcaagcgta taccgctctg gcgatggcgt
    35341 ctgccagttg tgcttctttc gccgcctggt tgtacaacat gccgtgcggt ttgacatgac
    35401 gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc gccaatttga tacagcgtct
    35461 gggcgtaaac ggtttccggc ggcagctgca tggcgctgcg accaaaattt tccctgtcgg
    35521 gaaaactcgg gtgagcgcca atcgcgacac cattttttat tgcttcacgc acgcaagcct
    35581 gcatggtttg cgcatcgcct gcatgaaatc cacaagcaat attggcagag gaaaccagcg
    35641 ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg tcaattttca
    35701 ttgtgcagcc gccacgctaa ttgttcaaaa taacgttgct gatcttgccg cgctttcagt
    35761 gcctcttcca gtgaacactg gacaaaatga atcggctgac cgagcggaat ttgcgccaga
    35821 tggtacatat cagcctcaat gatacaggca atacgtggat aaccaccggt ggtctgtgcg
    35881 tcgttcatca acacaatcgg ctgcccgtta tgcggcacct gcaccacgct cggtaacaaa
    35941 ccgtgagata acagttcgcg atcggtgatg cgttttaaaa tttgcccctg taaacgatag
    36001 cccattcggt tactttgcga gctaagctgc cagggcgaac gccagaatgc atcctgcgag
    36061 gcgcgatcga actcatgata ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc
    36121 agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg atttgccaat cgggagtcag
    36181 tcaccatcct tcagtaaacg gccttccagc ccgccaatcc ccactttgag atcggtgctg
    36241 catgacccca ttaccggcgg aacatcaata ccacccgcga ccgccagata actgcgcatc
    36301 ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg
    36361 gtccagacgg cgttatcatc caaccgcgct tcgcaacctg cacccgtcag ggcaaaccac
    36421 ccatcagttt cgaactcaac tgttaactga ccgagcgtga tctccagcgc gggggcattg
    36481 gcgtcattac ccaccagcag gttagcaatg cgcagcgcag gcatatccag tgcgccacag
    36541 tggctgatac ccgactggca aaaaccgtga cgaccgccat cctgcacagt ggtgtacatg
    36601 cccgcacgaa taatcttcag catattccct ccttctgcgg tacaaatcgc acgctgtctc
    36661 ccggacgtaa taagatgggt tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac
    36721 caatcaactg ccagccaccc ggcgttgcca gcggataaac accagtctgc ggcccgccga
    36781 tcccgacaga acctgccgga acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt
    36841 gttccggcaa actcccgaga tacgggaagc ccggttgaaa acctaaaaac cagaccacgt
    36901 attccacgga ggagtgcaat tcaacaacct gtttttcgct caacccgcaa tacgccgcga
    36961 ccaccgccaa atccggtccg cctgcaccac cgtaaaccac cggaatttca ataaagcgag
    37021 actcaggttc cagggcctcg ctctcctccc accagcgttg caaacgctct atggcatcca
    37081 gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt
    37141 caaccacatt cggcatatcc accagacgct gcgccagtcg ccagatccgt ttctggctag
    37201 ccagcgtcac cggcggttcc agttccagca ctaccgccgt ttcacctatc agataacaac
    37261 gcgctcgttg cactttcgat ccctctgatt attcgttatg caggattagg aatatcaata
    37321 aaggtcacat caagatcggt attttcattc agccactcgc tcaatgcgcg aataccaccg
    37381 cgttcagtgg catggtgacc tgcagcataa aaatgcaatc cctgctcgcg ggctgaatga
    37441 atggtctgtt cagaaacttc gccagtaata aaagcatcca cgccaaaacg cgcggcgcta
    37501 tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc gctggaccac ctcaggtccg
    37561 gtatcgccac accataacgg cttacgtccc agacgcgctt caatccagga ggctagttcc
    37621 attcccggca caggcatggt cagctctccc cacggcacca acggctcaat ttcgcccatg
    37681 accgtgatcc ccagtaacgc cgccagctgt gcgttattgc ccagctcagg atgtgcgtca
    37741 agcggcaaat gccagccata caggttgata tcattcgcca gcaacgtttt taaacggtta
    37801 cgcttcattc cgcgaatgac cggagactct cctttccaga agtagccatg atgcacaatg
    37861 accgcatctg cgcccagacg cactgcctca tcgagcaaag cctggctggc ggtgacaccg
    37921 gtaacaattt tttgcaccgt ctctttgcct tccacctgca aaccattcgg cgcgtaatca
    37981 ctaatcgccg cgctgttcag tttttcgtta atcagttgtt ccagttcggt gtttttcatc
    38041 ttcgcgcctt ccgtattctg atagctacac cgttaacata gcgttacatc gcctgttcgt
    38101 cgatagccaa tcttttgaca tattaagaaa caaacccgac gccaaaagac gcaaattgtt
    38161 tatatcatca cgcttcagct aatgtatatt tatgcaatca gacagcatat tgcataaata
    38221 atcagatctg tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg caactattac
    38281 gtgacgttgc cgtcaattgt ggaattatta tgaataaaca cgcatcacaa ccgcgcgcta
    38341 tttactatgt cgttgcgctg caaatctggg aatattttag cttttacggc atgcgtgccc
    38401 tgctgattct ctatctcacc aatcaactaa aatacaacga tactcacgcc tacgagttat
    38461 ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt ttggcggata
    38521 aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg atcggtcatg
    38581 tggtgctggg tgccagtgag atccatccgt cattcctcta tctgtccctg gcgattatcg
    38641 tctgcggcta tggcctgttt aaatctaacg taagctgtct gctcggcgag ctgtatgagc
    38701 caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta tgcggcgggt aacgtgggat
    38761 ctattatcgc acctatcgcc tgtggttacg cccaggaaga gtacagctgg gcgatgggct
    38821 ttggcctggc ggcggttggc atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc
    38881 atttcactca tacccgcggc gttaacaaaa aagtactgcg tgcgacaaac tttctcctgc
    38941 cgaactgggg atggctgctg gttctgctgg tggcaacgcc tgcactgatt accgtgctgt
    39001 tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac tattattggt ctgggtgtac
    39061 tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg gaaggagctg gggctgattg
    39121 tgacgctcac cttcttcagt atgttgttct gggccttcgc acaacagggc ggtagctcga
    39181 ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc gttccgaccg
    39241 cgatgttcca gtcgattaat gccttcgcag ttatgctgtg cggtgtgttc ctggcgtggg
    39301 tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg
    39361 gccttggcct gatgagcgcc ggattctgca ttctgacctt aagcgcccgc tggtccgcaa
    39421 tgtatggtca ctcttctctg ccactgatgg tgttaggcct cgcggtgatg ggctttgcgg
    39481 aactgtttat cgacccggtt gccatgtcgc aaattacgcg cattgaaatt ccgggcgtga
    39541 ccggcgtatt aaccggcatc tacatgctgc tttctggcgc gatcgcgaac tatctggcgg
    39601 gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg atcaactact
    39661 ccatcaatgc atatattgaa gtatttgatc aaattacctg gggcgcactg gcgtgtgtag
    39721 gactggtact gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc
    39781 tggagtcata ataggccagc accgatgcct gatgcgacgc ttgacgcgtc ttatcaggcc
    39841 tacgtatttc ctgcaattta ttgaatttgc acaaatttgt aggctggatt aggcgttcac
    39901 gccgcatccg gcatctgggc tcgattgcct gacgcgtctg ttatttcccc ttccgcgccg
    39961 cctcatacgc tgccaacgtt tgtactctcg cctctttgtg ctcgactatc ggttgcggat
    40021 aatccagctt cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg
    40081 gtacatcgcg cagctccggt aaccatcgac gaataaactc gccctcacgg tcaaatttct
    40141 cgccctgagt tatcggattg aaaatacgaa aatacggcgc agcatcagta cccgtagaag
    40201 ccgcccactg ccagccaccg ttattagctg ccaaatcacc atcaatcagc tgcgacatga
    40261 aatatcgctc gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgtaa
    40321 tcatccgtag ccggttatgc atccagccag tgctgttaag ctgacgcata gcggcatcaa
    40381 taatcgggta tcccgttttg cctttctgcc aggcctgtaa atgtgcggga ttgctttgcc
    40441 actgcacacg atccgtccag gcaataaatg gacgatgttt acacaacgag gggtaatacg
    40501 tcatcagatg acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc
    40561 caccgtccag cgcctgcggc tgttccgcca acaagcgatg caaacactgg cgaggcgata
    40621 acccgcccgt tgccaagctg gcggacaaac ggctggtgcc ttccactgcc ggaaaatctc
    40681 gttgttgctc atactctcca gcaccgttct cgcaaaactg gcgtaattgc gcaatcgccg
    40741 ctttttcttc caccgggaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa
    40801 tggatggcgc gggctttatc gatccgctac tacgaacttt tggtgcagcg acgcactccg
    40861 gcatcccttc ccgcagccgt ttcagccagg cattcttaaa aggcgtaaag actttgtaca
    40921 tctcgtgatt gccggtcatc accgcccccg gcggcaggat cacgctgtca tcaaatcctt
    40981 cacacaccac gttacgcagc gctctttcaa cgtccacatc ccgcgctcgc tcattcacct
    41041 catactgata gttataaaaa agatgagtaa cgctgttttc cgcgcacacc tgtttaacta
    41101 tttcgacact ggcgacaaag tcatccactt cacggaacaa taaaggaata cctttttccg
    41161 ccagcgcgat ttgtagccca ttcagttgag cattgataag ttcagcctga cgcggcgaca
    41221 tgttatgcgt ctcccactgg cgtggtgtag cgatatacag cgccagcacg cgtgcagacg
    41281 aattgcggca ggcggcagcc agtgcgagat tatcgtgcag acgtaaatcc tggcgaaacc
    41341 aggccagatg ggtagtcata aaactcctga atggcgcaag ttaatcacac gcggccaaag
    41401 ttcgaaataa cgctgccctg aaagccaggc gtcaggataa agcgccatat agtgtttgat
    41461 acgcatcagc ggcgcaagaa gtggttgctc gccacggcga taactgtcga taagcgcagc
    41521 cagctgctgg cgttctgtgg aatcaatatg cgggcgaaaa taaccctgaa cgtgcatcaa
    41581 aacgttggtg tgatcgcgcg ggttggcggg atgagaaagc aaaacaatta cgcgttggcg
    41641 gtactggttg taaaaatcat ccagattttg ccactgatga atatcagcca caaacgggcc
    41701 aagtttgcgg tactcgggct gagaatgggc cagaaagacg agtttatagc ggctgtgata
    41761 atcgagaagc gccccgcgcg ttaagccatg ttctttcagt cgatgtaact catgaagggc
    41821 gaagatacgg attagggtgc tgtcatcaaa tcgttgtaga ttcattactg ctcctgaacg
    41881 gtatacagga gtaagtgtag gcgttggtaa atgatgtttg tgaagttaat ccgctagcag
    41941 ggcaagatta cgaaagcgcg ctccccgcaa gcaactaatc aaggtagtta atcatggctg
    42001 aacacgatat tacgccagaa gtcaccattt ccaaaacaca gcgccgctat aaagtcggct
    42061 atgtcagagc ccgccaccag gaccgcaaaa ccggtatagc ccggtattat tcacaacatc
    42121 ccagcctgca ccttaagggc gactggctga aagaggccgg atttgatacc ggacggggtg
    42181 tcacggtgaa gatttcggag gggggcctga ccatcatcgc tgacagtgac gaggtgcagg
    42241 aactgcggga tgggatgttt agtgtgctga acgaaagtta aatattaaaa aagctgggaa
    42301 gatttaaaac cttcctagct ttttattctg tcttattata atggatactc atcatcttcc
    42361 gatgaattta taaattgctc caagtttaca tgaagagtac ttaacctagt ttttaactca
    42421 ttaagtgaag cgataatttc atattttcca acataatcat atgatggatc gtattcatta
    42481 tctatgtcag aagctaaaaa tgatgatatt cgtagtttaa atttagcttc atctttatta
    42541 cattcattgg cataaggtga atgccctatc atagcatatt catctaatct aaaaggataa
    42601 gatggtttta attcatcgat caatgaaatt tcaatccatg ttctatatcc aggcattaat
    42661 ggactaattt ctttgagcaa aacctttcca cttaataatg tattgagatt gttcattatt
    42721 atgtctccca cgggaaaaat atatgtctaa cacttccacc atgttgatga acttgtaaat
    42781 gtggtaaatc accatcagga tttcctagtc catgacctga cactctatat gaaggttttg
    42841 ttacagaagg atcttttata tcatcccaat gatatgaacc tgcttttgtt ttatttccag
    42901 taagatattc cattaagttc ttagtgctgg ggcctgactc acctgtcata tttttataat
    42961 tatgacctaa atatctatcc ataaacaatt gttcagcgtc acttctagac ctaacagtaa
    43021 ctttggtttc actactatct aataaacgat tcgctttttg gttactaaca gcacatggag
    43081 ttaatccaag tggatcagca tattttagag gattaggtgc ataactgtag agatttaaac
    43141 cgcctctcaa ccctatcggg tcagttacca tgaaccgccc gacatccgga tcatagtacc
    43201 tgtgcagatt gtagtgcagc cccgtttccc tgtcgaggta ctgtccctgg tagcgcaggt
    43261 tctgctccac cccgccgtgg tccttctcct gtatcgtgtt accccagacc tgataccccg
    43321 tctcccacac gattttcccg tcgctgtccg tcatctcttc cggcgcgccg ttcacatccg
    43381 tgtgaaaata cagtatccgg ttcgggccct ctttgcccgc cttatccacc cgcgccagcg
    43441 gttcataact cccctggtcc ctgtagatgt acaggctgcc tgttccctcc ggcctgctct
    43501 cctgcgccag ccgcattccg tcccataaaa attttgtccg gttgtacggc ttaccgtcag
    43561 ggctgatgtg ctgtttttcc gtccgcctcc ccagtgcatc gtacacatac cggtatgtcc
    43621 cccgcgctgt cgtcacttcc accatccggt gttccgcatc ataacggtaa ctctgcgttc
    43681 cgctccggcc ctccttttcc accgcacggc caaattcgtc ataacggtag tgcacaccac
    43741 ggtaagacat cagccggttg aacggcacca cgctgttttc tgccgtggcc ctttcccccg
    43801 cgcgcctccc cagcaggttt cccgccgcgt cgtactgcca gctcgccaga tacccctcat
    43861 cctccgaacg acacgccgtg atgcgccccg tggggtcgta gaagtaatcc gtttcccccc
    43921 gccggctgtg ccgcgtcttc agcagttcat cctcgccact gtagctgtac tgccggtccg
    43981 tgaacgtctc cggtaccacc ccgcgcatcc cgctgtatgt ctccctgcgc ttcagccgcc
    44041 cgccggggtc gtaaagctgc cgcgtgctca gtcgcccctg gcttctgagc acttcacggt
    44101 gcaggtggtc gcgcgtgaac tcgctgatga cctcccggtc cagatttatc tgctggagat
    44161 ggccgctgcc gtagtacaga tggcgctgcg ttctgccgtc cggaaaggtg gtggcagtcc
    44221 ggttgcccag tttatcgtac tggtgggtca ggatgccctg cgccgtctcc tcgctcagca
    44281 tccgccccag tctgtcgtag ctgaagcgga taacttccgg cgcgtcagtt tcgtgccgct
    44341 ggcgacggat ttccagtagc tggtctgccg catcatggac gtattccgta cggccatcgg
    44401 ctgtctcttt tgccgtgagc cggcccgccg catcacgcgc cagccggtgg cggatttcac
    44461 cgccccggtc cccggtgaac accacttccg tgaccgcgtt cagcgcgttg tagcggtacg
    44521 actggcggca gccgtccggg tttatctgct ccgccagacg gtccagcaca tcatggcgga
    44581 accggtagcg ttctccgttc tcgttcgtga gcgtgaccag acgcccgtaa gcgtcgtacc
    44641 cgaatgccgt cctgcgcccc atcgcgtccg tccggctgcg cacccggccc tgcccgtccc
    44701 gctgccagcg cgttatcctc cccgtcgggc tggtgtactt taccagcctg cccgccgcgt
    44761 ctgactggta attcaccgtg cggccgtccg ccagtatcac ctccgtcaga tgaccgctgt
    44821 cgtcataccc gtagcgcgtg ctgtggctct gcgcatccgt cattctgata agcctcgtgc
    44881 gttcgtcgta gaaccacgcc gtctgcgagc cggaacagtc cgtgtgccgc atgagctgcc
    44941 cgtcttcatt ccactggagg tatttattcc ccccgcgcgc atccgttatc tgcaccacct
    45001 agccgtggtg gtcatactga taccgggtgt tctgctgcag cgggtcggtc acggcaagaa
    45061 ggttgccgcg ttcatcgtac acacagcgcc aggtgtttcc cgccgcgtcc acctccgttt
    45121 ccggctggtg ccacaccggg tgccactgcg cctgctccac ccgccccagc gggtcgtgcg
    45181 tctccgtgat atgcccctgc aggtcataca catagcgcca tttgccgccc tgagggtcgg
    45241 tcatccccag caacagtcgt tcttcgtcgc tccagcggaa tgtcgtcacc tgtccggctt
    45301 cgtcctggta cgccgtcacc tgattctgcg catcccactg gtgataacgt gtgcgcccca
    45361 gtccgtccgt cacgcacagc ctgcgctgcg ccaggtccca cgccagccgg tactgttcac
    45421 cgctgtccgt gtggtgcccc gtcacccgcc atgccgcgtc atccggacct tcataccagc
    45481 cccactggta ataataccct tcgccgcccg gctggcggtg cagcgtcatg cagtggtttt
    45541 cgtcgtaagc aaactcacgc agcaggtgcc ccgtgctgtc gtacacgccg ttcaggtggc
    45601 cggcctcgtc atagctgtaa cgacgcagca gtaaaggtgc agtctcaggg aaatgctgat
    45661 aaatctgtgt cacccgccgg ggatgcgccg ccagttcgta atacagacgg atgcacggac
    45721 gctgctgttc gccgctgatt tccacaatgc ggccccggtc gtcgtagtac agattcagac
    45781 agttgctgtt acggtcgccc agcattttca gccgctggcg ctgcgggtgg tgcgggtcag
    45841 cctcaaacag aaaacactgc ccgtcatcgc tgctgataag ccagtgcccc tgctcaccac
    45901 gccttaccgc cagcccttca ccggggctgt aaaacccgcc ccccggcagt acggcttcca
    45961 tatccaagcg tcgccccatg cccgacacaa aggtcatgca gttttcgtct gcgctacccg
    46021 gtgtgcacac gaggctgact ttatacatta cactccagcc ctgaccaaac attccttccg
    46081 tacgttcgtc acgactgctg tacacgcgct gccagtccag cggaaagtgg cccggcagac
    46141 tgaaatcgaa gtcatcgtca tcggccaggt atttggcccc ggtggcggca ttgaccggat
    46201 gcggaagggc actggtggcc ttctgcgtca gcatgctcat cccgacactg gcagcaaaac
    46261 aggcgatttt agaaagcatt ttgcccgggc gaagaaacat cagcgccacg gttgccacca
    46321 gggttatctg cgacctgccg cttctgatat ccctgaccac cagtaacgga ccgccaatgc
    46381 gcacatccgg tgacacaaac gccccgttcc ccggctcatt caccaccctg gcctcgcagg
    46441 tacagcgtgc cccgctgcgt gtcgccggct ggctgttgat acgcacatcc cgcgcgcctt
    46501 ccgcgatata ttccgctgcg ggcggctcgt catcgccttt gaacagatta ctgatgctgc
    46561 caataaccgc attcactccc tgaaaggcgg tttccagcat accgcccgga ttcagcaggt
    46621 taagggccgt tcccaggggg tcatccagaa aggcggcttt tttttgtgcg agaaactgcg
    46681 tcagtgagga aggatgttta tggcagtcga ttttatcgtc aggggtcggg gtggatcccg
    46741 gggccgcact ggccacagtg ggctgccata gttccgacac aaaatgtgtc acgccatcca
    46801 ccatttcaga acgcccttct tccgtactga gctggtcgtg cagagcccgg gacatcagca
    46861 caggccctgt caccgggtta accgccaggg ctatggcccc cgccatttcc agatattccc
    46921 cggccacatc ccggaacgtt ctggcatcct cctgacgctg cgcttcttcc tgttctgcct
    46981 tcgcctgcgc ttcctgcgcc tctatgtcct gccgcgatgc cgcagtcgcc gctgcccgcg
    47041 cggcaggttt gctgttgatc catgtgtcac cagagccggt gaggatgtaa ccttcaatct
    47101 gaggcgggaa aagcatattt gcgagggctg aacaggcttc gctgaccttt tctgtcacgc
    47161 cggtgtacac catcgccaca cccgccacaa aaccggtcag cacgacggcc gtgcacccca
    47221 tcgtgatccc cactgacgtc gccagtaacg aggttgtgcc gacaatgagg gcatcaataa
    47281 cgacatttgc ggccacctcc agcaccgccc ccagcacatc tgccataaac gacgtatgca
    47341 gcagggcatc accctgtctt gccgcccaga atgcctcttc catgtcccgt tccctgtatg
    47401 attaattcgt cctgacttcc tgccgcatcc tgaagcgcag attcttttta atttcagccc
    47461 agcgcgtcat atctgccggg gtcagcggtg tggttctggc gtaggtgaag gccatcatga
    47521 cccgctcacc cggcaataca aatgccacct gacactggta cacctgctcg ttaccccgga
    47581 gaaactggct ctgtatttcc aaaccgcctc gttgagataa tattccgtca taatgtaaat
    47641 agtccgtttt aataaaggaa ttatttattg acaggttctg tttttttaat catgaatgta
    47701 atacccatca ccgctttaca gcacacttta tctttccagc cataatcact aataatatta
    47761 agcaattagc aatatttaca acgaccagag cggatacata gttatgtatt taacaatccc
    47821 aatatattca ctatgcaaat aaaaccacta gtaacagaca cgctaccgcc tccatataaa
    47881 aatggacacc ttataaaatt gtatcttgca tagaaaaagc tgaaatatcg gatgcgtttt
    47941 aattatcaac tataaatcta taagcttttt attaacaa
//
LOCUS       DADLDT020000014        47944 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000014 DADLDT020000000
VERSION     DADLDT020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47944
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.014"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1227
                     /locus_tag="JQH22_000766"
     CDS             <1..1227
                     /locus_tag="JQH22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFB2205185.1"
                     /translation="EFELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLEN
                     HPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYM
                     IIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGN
                     IALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELV
                     YVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDK
                     NFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPE
                     TVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAE
                     KYLLAQFGVDIGEAAQ"
     gene            1224..2189
                     /gene="yggF"
                     /locus_tag="JQH22_000767"
     CDS             1224..2189
                     /gene="yggF"
                     /locus_tag="JQH22_000767"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFB2205186.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2211..2720
                     /gene="fumE"
                     /locus_tag="JQH22_000768"
     CDS             2211..2720
                     /gene="fumE"
                     /locus_tag="JQH22_000768"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFB2205187.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2717..3430
                     /locus_tag="JQH22_000769"
     CDS             2717..3430
                     /locus_tag="JQH22_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFB2205188.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3402..4084)
                     /locus_tag="JQH22_000770"
                     /pseudo
     CDS             complement(3402..4084)
                     /locus_tag="JQH22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4078..4755)
                     /locus_tag="JQH22_000771"
     CDS             complement(4078..4755)
                     /locus_tag="JQH22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFB2205189.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4743..5126)
                     /locus_tag="JQH22_000772"
     CDS             complement(4743..5126)
                     /locus_tag="JQH22_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFB2205190.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5142..5450)
                     /locus_tag="JQH22_000773"
     CDS             complement(5142..5450)
                     /locus_tag="JQH22_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205191.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5451..6029)
                     /locus_tag="JQH22_000774"
     CDS             complement(5451..6029)
                     /locus_tag="JQH22_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFB2205192.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6052..6483)
                     /locus_tag="JQH22_000775"
                     /pseudo
     CDS             complement(6052..6483)
                     /locus_tag="JQH22_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6855..7874
                     /gene="epd"
                     /locus_tag="JQH22_000776"
     CDS             6855..7874
                     /gene="epd"
                     /locus_tag="JQH22_000776"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFB2205193.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7924..9087
                     /gene="pgk"
                     /locus_tag="JQH22_000777"
     CDS             7924..9087
                     /gene="pgk"
                     /locus_tag="JQH22_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFB2205194.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9302..10381
                     /gene="fbaA"
                     /locus_tag="JQH22_000778"
     CDS             9302..10381
                     /gene="fbaA"
                     /locus_tag="JQH22_000778"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFB2205195.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10572..11432
                     /gene="mscS"
                     /locus_tag="JQH22_000779"
     CDS             10572..11432
                     /gene="mscS"
                     /locus_tag="JQH22_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFB2205196.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11571..12206
                     /gene="argO"
                     /locus_tag="JQH22_000780"
     CDS             11571..12206
                     /gene="argO"
                     /locus_tag="JQH22_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFB2205197.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12299..13039
                     /locus_tag="JQH22_000781"
     CDS             12299..13039
                     /locus_tag="JQH22_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFB2205198.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13206..14156
                     /locus_tag="JQH22_000782"
     CDS             13206..14156
                     /locus_tag="JQH22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2205199.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="JQH22_000783"
     CDS             complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="JQH22_000783"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFB2205200.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="JQH22_000784"
     CDS             complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="JQH22_000784"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFB2205201.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="JQH22_000785"
     CDS             complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="JQH22_000785"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFB2205202.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="JQH22_000786"
     CDS             complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="JQH22_000786"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFB2205203.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19723..20616)
                     /gene="argP"
                     /locus_tag="JQH22_000787"
     CDS             complement(19723..20616)
                     /gene="argP"
                     /locus_tag="JQH22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFB2205204.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20999..21658
                     /gene="rpiA"
                     /locus_tag="JQH22_000788"
     CDS             20999..21658
                     /gene="rpiA"
                     /locus_tag="JQH22_000788"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFB2205205.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21914..23146
                     /gene="serA"
                     /locus_tag="JQH22_000789"
     CDS             21914..23146
                     /gene="serA"
                     /locus_tag="JQH22_000789"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFB2205206.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23375..23434
                     /gene="ibsC"
                     /locus_tag="JQH22_000790"
     CDS             23375..23434
                     /gene="ibsC"
                     /locus_tag="JQH22_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFB2205207.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23767..23826
                     /locus_tag="JQH22_000791"
     CDS             23767..23826
                     /locus_tag="JQH22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFB2205208.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23927..24529)
                     /locus_tag="JQH22_000792"
     CDS             complement(23927..24529)
                     /locus_tag="JQH22_000792"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFB2205209.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24550..24733)
                     /gene="ssrS"
                     /locus_tag="JQH22_000793"
     ncRNA           complement(24550..24733)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JQH22_000793"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="JQH22_000794"
     CDS             complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="JQH22_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFB2205210.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25272..25850
                     /gene="ygfB"
                     /locus_tag="JQH22_000795"
     CDS             25272..25850
                     /gene="ygfB"
                     /locus_tag="JQH22_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFB2205211.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25876..27201
                     /gene="pepP"
                     /locus_tag="JQH22_000796"
     CDS             25876..27201
                     /gene="pepP"
                     /locus_tag="JQH22_000796"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFB2205212.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27198..28376
                     /gene="ubiH"
                     /locus_tag="JQH22_000797"
                     /gene_synonym="visB"
     CDS             27198..28376
                     /gene="ubiH"
                     /locus_tag="JQH22_000797"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFB2205213.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28399..29601
                     /gene="ubiI"
                     /locus_tag="JQH22_000798"
                     /gene_synonym="visC"
     CDS             28399..29601
                     /gene="ubiI"
                     /locus_tag="JQH22_000798"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFB2205214.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29675..29758)
                     /locus_tag="JQH22_000799"
     CDS             complement(29675..29758)
                     /locus_tag="JQH22_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205215.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30049..31143
                     /gene="gcvT"
                     /locus_tag="JQH22_000800"
     CDS             30049..31143
                     /gene="gcvT"
                     /locus_tag="JQH22_000800"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFB2205216.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31167..31556
                     /gene="gcvH"
                     /locus_tag="JQH22_000801"
     CDS             31167..31556
                     /gene="gcvH"
                     /locus_tag="JQH22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFB2205217.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31675..34548
                     /gene="gcvP"
                     /locus_tag="JQH22_000802"
     CDS             31675..34548
                     /gene="gcvP"
                     /locus_tag="JQH22_000802"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFB2205218.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34815..35557
                     /locus_tag="JQH22_000803"
                     /pseudo
     CDS             34815..35557
                     /locus_tag="JQH22_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="JQH22_000804"
     CDS             complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="JQH22_000804"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFB2205219.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="JQH22_000805"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="JQH22_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="JQH22_000806"
     CDS             37566..38225
                     /locus_tag="JQH22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFB2205220.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="JQH22_000807"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="JQH22_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFB2205221.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="JQH22_000808"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="JQH22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFB2205222.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="JQH22_000809"
     CDS             39891..40298
                     /locus_tag="JQH22_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFB2205223.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="JQH22_000810"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="JQH22_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFB2205224.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="JQH22_000811"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="JQH22_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFB2205225.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="JQH22_000812"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="JQH22_000812"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFB2205226.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="JQH22_000813"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="JQH22_000813"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFB2205227.1"
                     /translation="MKQQIQLRRREVYETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="JQH22_000814"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="JQH22_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFB2205228.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="JQH22_000815"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="JQH22_000815"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFB2205229.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="JQH22_000816"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="JQH22_000816"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFB2205230.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="JQH22_000817"
     CDS             47703..47774
                     /locus_tag="JQH22_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205231.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="JQH22_000818"
     CDS             complement(47771..47896)
                     /locus_tag="JQH22_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205232.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
BASE COUNT        11472 a        12014 c        12962 g        11496 t
ORIGIN      
        1 gaatttgaac tgccagaaat taccgataat gaactgttag tgagtgtaat ttctgacagc
       61 gtctgtttat cgacctggaa agcggcgtta ctcggtagtg aacataaacg cgtacccgac
      121 gatttagaaa atcatccggt cattaccggg catgaatgtg ccggggttat tgtcgaagtg
      181 ggtaaaaatc tcactggcaa atataaaaaa ggccagcgtt ttgtattgca accggcgatg
      241 gggttaccaa gcggatattc agcgggctac agctacgaat attttggcgg caatgccact
      301 tatatgatta ttcccgaaat cgccattaat ttgggctgcg tattaccgta tcacggctct
      361 tattttgctg cggcgtcgct ggcagagcct atgtgctgca ttattggtgc ttatcatgcc
      421 aattatcaca ccacgcaata tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat
      481 attgcactgc tggcgtgtgc gggtccgatg ggcattggcg ctatcgatta cgccattaac
      541 ggtggcatac aaccgtcacg ggtggtggtg gtcgatatcg acgacaaacg cctggcgcag
      601 gtgcagaagc tgctgccggt ggatctggcg gccagtaaag gcattgagct agtgtatgtg
      661 aataccaaag ggatgagcga tcctatccag atgctgcggg cgctgacagg agatgtcggg
      721 ttcgatgaca tttttgttta tgcggcggtg cctgctgtcg ttgagatggc tgacgaatta
      781 ctggcggaag atggctgtct gaacttcttt gccgggctga cggataaaaa cttcaaagtg
      841 ccgtttaatt tctacaacgt ccattacaac agcacgcacg tagtcggtac atccggcggt
      901 tcaacggacg acatgaaaga ggcgattgcc cttagcgcca caggacagtt acagccgtcg
      961 tttatggtga cccatattgg tggcctggat gcggtgccag aaaccgtgct caatctgccg
     1021 gatatccctg gcggtaaaaa actcatttat aacggcgtga ccatgccgct cactgccatt
     1081 gccgattttg ccgaaaaagg caaaaccgat ccgctgttta aagagttggc gcggctggtt
     1141 gaggaaacgc acggcatctg gaatgaacag gccgagaaat atctgctggc acaatttggc
     1201 gttgatatcg gggaggccgc gcaatgatgt ccctggcgtg gccattattt cgcgttacgg
     1261 aacaggcagc gttggctgcc tggccgcaca ccggatgtgg cgacaaaaat aaaattgatg
     1321 gcctggcggt caccgcgatg cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg
     1381 ttatcggcga aggagaaatt gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca
     1441 aaggcgatgg gccagaagtc gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg
     1501 cgatggggca gagcaatgcg ctggcggtca tggctttcgc tcctcgagat agcctgttac
     1561 atgcgcccga tatgtatatg aaaaagctgg ttgttaatcg actggctgcc ggggcgattg
     1621 atctgtcttt gcctctggcg gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc
     1681 tggacaagct gcgcatggtt acgctggata aaccgcgtct gagtgccgcg attgaagaag
     1741 caacacagct gggcgtgaag gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc
     1801 tgacctgctg gcaggataat ccttacgatg tgatgtacac catcggcggt gcgccagaag
     1861 gcgtgatttc tgcctgtgcc gttaaagcgt taggcgggga tatgcaggcc gagctgattg
     1921 acttttgtca ggctaaaggg gattacacgg aaaatcggca gattgcagag caggagcgca
     1981 agcgttgtaa ggcaatgggc gttgacgtta accgtgtgta ctcgctcgat gaactggtga
     2041 gggggaacga tatcctcttt agcgctacgg gcgtgacggg cggcgagttg gtgaacggta
     2101 tccaacagac ggcgaatggg gtgcggacgc agacattact gatcggcggc gcggaccaaa
     2161 cgtgtaatat aatagactcc ctgcattgat ggtgatctat cggaagacca atggcgacgc
     2221 tgacagaaga tgatgtgctt gagcaactgg atgcacagga caatttattt tcatttatga
     2281 aaactgcgca ttctattttg ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg
     2341 ataacgatga agagatcgtc gaatatgcag tgaagccgtt actcgcccaa agcggcccgc
     2401 ttgacgatat tgatgttgcg ctgcgtttga tttatgcgct ggggaaaatg gataaatggc
     2461 tgtatgccga tatcacgcac ttttcccagt actggcatta cctgaacgaa caggatgaaa
     2521 cgcccggatt tgccgatgat ataacctggg attttatcag caatgtcaac agtataactc
     2581 gcaatgcgac gctctacgat gcgttaaaag cgatgaagtt cgccgatttc gccgtctggt
     2641 cagaggcgcg ttttagcgga atggtcaaaa cggcgctgac gctggcagta acgaccacct
     2701 taaaggaatt aacgccgtga aaattgaatt aacggtgaat gggctgaacg tgcaagcgca
     2761 gtaccatgat gaagagatag aacgcgtgca taaactgttg ctgcgtatgc tggcggcctt
     2821 acagacggta aatccgcagc ggcgtacggt ggtttttctt tgcgctccgc cggggacggg
     2881 taaatctacc ctaaccacct tctgggaata tctcgcgcaa caagacccgg agctgcccgc
     2941 tattcaggcg ctcccgatgg atggttttca ccattacaat agctggctgg acgcgcatca
     3001 attgcgcccc ttcaaaggcg caccagagac atttgacgtt gcgaaactgg cggaaaatct
     3061 gcgccaggtg gtggaagggg attgtacgtg gccgcagtac gatcgacaaa agcatgatcc
     3121 tgttgaagat gcgttgcacg ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt
     3181 gctggacgac gagaagtggt gccagcttgc gcaattttgt gatttctcga tctttatcaa
     3241 cgcgccagct gctgcgttgc gggagcgtct ggtggggcgc aagctggcgg gaggggtatc
     3301 gctggcagat gcagaagcct tttatgaccg taccgacggg ccgaatgttc gccgggtgct
     3361 ggaagagagt ctgcctgcaa atttgacctt aatgatgacc gctacgggtg aatatcgtct
     3421 ggtggattaa cgttcctgat tacaccatct tccagccgca ccacacgaga gaaatgacgc
     3481 cgggtaaatg cggcgtcatg actgattgct accacgctcg tgcctcgctg gcggcacttc
     3541 tccagccagc tttcaaacac gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc
     3601 agcagtaata gtggcggaga gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc
     3661 gcactatgca gatccaacgg gtgcgagttg gcagtgtctg ctaactgaca acattgcagg
     3721 gcagcatggg tacgttgagt gatttcatcc gccgggcatt tttgcagttt caaaccaaat
     3781 gcgatctcgt cagcgacggt gctgtgaaaa agttggtttt ccgcttcctg aaacagaaca
     3841 gtacgccaac ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca
     3901 gcataaccgt accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt
     3961 tccctgcgcc attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa
     4021 tgtcgcaaag acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta
     4081 acatagtggc tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa
     4141 aggtgtgcga tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg
     4201 taagcgctgc aacagcattt cgctggcctg cggcgtcagg cggctaaagg cttcatcaag
     4261 aattaagagc tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc
     4321 accggagagt gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc
     4381 ggcatcaatc cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc
     4441 aacttcttct tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac
     4501 gagctggatg gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag
     4561 cgtccccgtg cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg
     4621 tgccagcgta cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac
     4681 atagtgaaag tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg
     4741 ttctaacgta accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa
     4801 cgcgctacgc gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg
     4861 atccgaaatg cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg
     4921 tgtgttagcg gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc
     4981 agcggcacgc cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc
     5041 tcaacgacca ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca
     5101 aacagagcgc gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa
     5161 acaatcgcca gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt
     5221 ggcgtgccgc ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc
     5281 agcgagaaca tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata
     5341 aggcagaaaa aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc
     5401 gtggtgcagg ccgccaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg
     5461 gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag
     5521 acaatcaccg ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc
     5581 gactccacca ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta
     5641 ccggtgacac cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc
     5701 gtaataatca cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc
     5761 agccaaccgg agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg
     5821 tcggtcagca atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga
     5881 ccaagcagta ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc
     5941 atgctggcca gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt
     6001 gcctgactgg aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac
     6061 gggggaaata tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag
     6121 ttcctcaagg ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat
     6181 atgaccgcca agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact
     6241 ctgttccagc gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc
     6301 ttcttgcccg gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta
     6361 ggcggcatga agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc
     6421 ggggaggagg cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat
     6481 cattggagta ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata
     6541 ttctgatata agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt
     6601 gattctgcaa agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga
     6661 taattgcttt ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct
     6721 gaagcgtttc agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg
     6781 taaggtttcc ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac
     6841 cttgcaggag atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta
     6901 atgtggttcg tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca
     6961 acgaactggc ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc
     7021 gttttgcatg ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc
     7081 gcgtattgca tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag
     7141 tcctcgactg caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg
     7201 gggccaaaaa agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt
     7261 acggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta
     7321 ccacgaattg cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg
     7381 gcactgtgac cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc
     7441 ctgacctgcg tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg
     7501 ccgctggtat cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac
     7561 gtgtgccaac cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa
     7621 aagccaatga agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag
     7681 ttgactatac ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca
     7741 ttgtcgatgg cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt
     7801 gcgataacga atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg
     7861 ttgctttcag gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc
     7921 accatgtctg taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt
     7981 gcggatctga acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct
     8041 tctctgccga ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac
     8101 ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac
     8161 tacctgaaag acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt
     8221 gacgttgctg aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag
     8281 aaagacgacg aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac
     8341 gcattcggta ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac
     8401 gttgcgtgcg caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa
     8461 gaacctgctc gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc
     8521 gttctggact ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac
     8581 acctttatcg cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt
     8641 gacgaagcta aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta
     8701 gcaaccgagt tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct
     8761 gacgagcaga tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag
     8821 aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc
     8881 aaaggtactg aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc
     8941 ggcggcgaca ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc
     9001 tccactggcg gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg
     9061 atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt
     9121 cagcgcgctt ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc
     9181 gacaagagag tgaatcccca ggagcttaca taagtaagtg actgggggga gcgaacgcag
     9241 acgcagcaca tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga
     9301 catgtctaag atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa
     9361 agttttccag gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac
     9421 tgactccatc aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca
     9481 gttctccaac ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca
     9541 gggtgctgct atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca
     9601 ttatggtgtt ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat
     9661 cgacggtctg ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc
     9721 ttctcacatg atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa
     9781 atacctggag cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg
     9841 tggtgaagaa gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc
     9901 ggaagacgtt gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc
     9961 tgcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac
    10021 catcctgcgt gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct
    10081 gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag
    10141 ctacggcgta gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt
    10201 tctgaactac tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg
    10261 cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac
    10321 ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta
    10381 agtcattcct ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca
    10441 aggtgattca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc
    10501 ttgtcagact gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa
    10561 aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc
    10621 tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga
    10681 tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga
    10741 tctcccgtaa aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta
    10801 ttatcgcctt tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca
    10861 ttgctgtact cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta
    10921 acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg
    10981 acctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta
    11041 ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga aatattatta
    11101 acttctcccg cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg
    11161 atatcgatca ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga
    11221 aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg
    11281 tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta
    11341 ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact
    11401 ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct
    11461 ggcctctctc ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac
    11521 aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt
    11581 attactttca aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg
    11641 cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg
    11701 ctatcagcga tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc
    11761 agtcgccgtg gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg
    11821 gttttggcgc ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc
    11881 tgaagcaagg cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc
    11941 atgtttacct ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac
    12001 caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg
    12061 ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca
    12121 atctggtagt gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg
    12181 ctcatgcaca agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct
    12241 aagcttgcct ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt
    12301 gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc
    12361 taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt
    12421 gccagacatt gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc
    12481 caagaaacag gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat
    12541 cgcgaaaaaa gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga
    12601 tggtaaaagt atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca
    12661 gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg
    12721 ttctgtgtcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc
    12781 gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg
    12841 gccggtatat agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat
    12901 gatgaaagcc gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca
    12961 gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa
    13021 aacacctgca gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc
    13081 cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    13141 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag
    13201 gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca
    13261 ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca
    13321 ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca
    13381 gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt
    13441 ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca
    13501 tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg
    13561 aaggcgtgac tgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg
    13621 accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg
    13681 aaaacctgac tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt
    13741 atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc
    13801 aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc
    13861 ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac
    13921 attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat
    13981 attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc
    14041 atcgttatac cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta
    14101 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    14161 gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat
    14221 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    14281 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg
    14341 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa
    14401 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    14461 gaccccggct acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt
    14521 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    14581 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    14641 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    14701 caccgattcc tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc
    14761 cagacgtgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    14821 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    14881 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    14941 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    15001 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc
    15061 cagttctgca acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg
    15121 cagcagccag gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc
    15181 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    15241 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    15301 acgtaaactg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    15361 tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt
    15421 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga
    15481 acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    15541 ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    15601 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    15661 tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    15721 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    15781 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccacttc cacaacatgg
    15841 ttgaggatgc cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc
    15901 tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac
    15961 gggacgccga ggtttacagg cgtcattgag aaggttgagg tactggcggc gatgatcaga
    16021 tcggaactca tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg
    16081 atcggtttcg ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag
    16141 agcggatcgc gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg
    16201 gatccacttg gcgcccgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac
    16261 gcctgcataa gatcatcaat aaagacttta cttaaggcat taagttttcg tccatagtta
    16321 aactcaatga ccgccacttt gttgatagtg gcaacgttca catactgata agacataaaa
    16381 attcctttaa tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg
    16441 cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg tggtaatagc gatcgaaatc
    16501 ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac
    16561 cgattgttgt tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc
    16621 gatgatggcg tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagacc
    16681 ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg
    16741 ccgggcaatg gcgacattgg tatggttatc gccatcgtct ttgttgataa cgagcagatc
    16801 agccacttcc atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg
    16861 caacgagata aaacagtcca ccatgcgggc gacttccgtt tccgactgcg cgacgccaac
    16921 cgtttcgaca atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgtgc
    16981 tcgctgactg gcaccgccca gatgaccgga ggagggtacc gggcgaataa acgccgcctc
    17041 ggcacgcgcc aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg
    17101 gctgctggga tcgaccgcaa taacagcaac ctttaatccc tcacgaatca acaacatgcc
    17161 aaaggcttca agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag
    17221 ggcgttacca cagaacggca taatagcgtc aagcagctgt gtacttagcg cctgatgacg
    17281 tgggtgacgg ctttccacca gcgtcatggc ttgggcgagc gtggcacgct caccctgacg
    17341 taagcggcga atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat
    17401 tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc
    17461 cgcgctcttg caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga
    17521 tatcttcgcg tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac
    17581 cggcagccag tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc aggcgggcga
    17641 tctcttcagg tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg
    17701 cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa
    17761 tgcgcgggcg acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga
    17821 actcggaggc cgatttctca gactgatgat agctttgcgc aatcacgccg gtaacacact
    17881 ggcttggcac cagataacgg tcgaaagcga cttccagcgc atcggaaatt tcacccaggg
    17941 tggcgcgaac gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg
    18001 cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc
    18061 gttccagcga agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg
    18121 tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt
    18181 cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt
    18241 cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt
    18301 tgacgatttg atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgt
    18361 ggcagagttc tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga
    18421 aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc
    18481 ccagcgtcgc agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt
    18541 cagtcaggct ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcggatcct
    18601 gtgcgccaaa tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga
    18661 cgttcataaa cagatccatg ccgataccga agaataacga caggcgagga gcgaagtcat
    18721 caattttcag tccggcagag attgctgctt tgatgtactc aatcccatcg gcgatcgtaa
    18781 atgctacctg ctgcacgcag ttggcacccg cttcccccat gtggtaaccg ctgatactga
    18841 tggtattaaa tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca
    18901 ttgacggttt tggtgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt
    18961 tttgaatggt gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat
    19021 aaaacgccag tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca
    19081 gcgggatctg gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt
    19141 tgccgacgtc acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt
    19201 caaacgcaac ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg
    19261 actcttttgc tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca
    19321 tagtggcacg cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca
    19381 gattatcgag atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct
    19441 gaaccagcga gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc
    19501 actcctgctc gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg
    19561 cttacggtga gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt
    19621 tttttgccgc ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg
    19681 aggacaacgc ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac
    19741 tttgtgacca taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc
    19801 aaagcggtgc cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc
    19861 accgctggcc agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg
    19921 gcgagcaagt tgtacgaacg cttctgaaga attaacgata tggcagggta cgctgccagg
    19981 aggaagatcg aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc
    20041 gaccactggc gctttcaata atgccgaacg cgttacgccg ttaggaaaat atttttcggc
    20101 aaacggtttt gagctgacga acagatagtc gagcgcaccg agtttatcga caagacaact
    20161 cggcagcgcc tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc
    20221 ctgagtacgg gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac
    20281 aggagccagt gccggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag
    20341 cagcagcgga gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac
    20401 ctggcgcagc agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg
    20461 caccaacagc ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga
    20521 ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc
    20581 cagtgcctgt aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat
    20641 gctttttcct gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg
    20701 ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt tgcgctattg
    20761 catttcgctt agcgacttcg cgccctggtt atgttgaatg gcgtggcgtt attgcctctg
    20821 ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg
    20881 atgcccgcca ttgcgcgata ttttatggat gagttaacca cccagcttgc caacggggtc
    20941 tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat
    21001 gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg
    21061 taccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggcac
    21121 gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa
    21181 aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga
    21241 tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac
    21301 ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg acgcctccaa
    21361 gcaggttgat attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag
    21421 tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt
    21481 gaccgataat ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc
    21541 gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg
    21601 tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc
    21661 tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca
    21721 cgcttttacc aggcaattgt cgattgctct aaataaatcc tctaaaccag catattcatc
    21781 caagaattac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc
    21841 aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga
    21901 caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt
    21961 agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga
    22021 atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt
    22081 catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact
    22141 ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa
    22201 gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt
    22261 gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg
    22321 tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat
    22381 catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta
    22441 tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc aggtacagca
    22501 tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc
    22561 caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct cgctgctgat
    22621 taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa
    22681 acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc
    22741 atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc
    22801 gactcaggaa gcgcaggaga atatcggctt ggaagttgcg ggtaaattga tcaagtattc
    22861 tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg
    22921 tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa
    22981 aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat
    23041 gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc
    23101 aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga
    23161 aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg
    23221 cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg
    23281 ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc
    23341 cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat
    23401 tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcaaggg aggagtcatc
    23461 ctcccttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg
    23521 cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc
    23581 cgtaatagat cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc
    23641 tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat
    23701 agctgggcag catggggctg taacggtaaa gccctcaacg aagcgagggc ttgaaggaga
    23761 agggttatga tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct
    23821 tattaacagt cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg
    23881 ttaaagaatc aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga
    23941 ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt
    24001 ccaccaactg acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt
    24061 gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg
    24121 cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca
    24181 gttttggctc atggatcttc aacctgttca tcaccagttc gctttgcgga tggtaattca
    24241 ggaacagcaa attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg
    24301 cgcgccagag ttgttctatt agcggctggg tgtcgagttc accatcaaaa gagaggaata
    24361 cagcgaccgt atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt
    24421 gacccatttc cagctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc
    24481 ggatttcttg tcgggataat gtcagtggga gctctggtag ttgcgtcatg gctggtgcca
    24541 gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa
    24601 ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgaa ccgagcatgc tcaccaaccg
    24661 cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac
    24721 atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc
    24781 aaagttttgg ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt
    24841 ctgctgcagc atccgaatac gctgttccat acttgccgcg tagtcacgag tctttgcttt
    24901 ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt
    24961 tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg
    25021 attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg
    25081 gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc
    25141 tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc
    25201 ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac
    25261 gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc
    25321 tgaaccaaca agggacgggt ctgacccctg ccgagatgca tggtttaatc agtgggatga
    25381 tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg acgaacgaag
    25441 gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg
    25501 atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca
    25561 gcgttttcga tcgggctgat gcactggcag gttgggtcaa tcacttcctg cttggtcttg
    25621 gcgttacgca accgaagctg gacaaagtga ccggcgaaac cggtgaagcc atcgacgatc
    25681 tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag cttgaaatgt
    25741 cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta
    25801 ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa
    25861 cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc
    25921 ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca
    25981 cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc
    26041 tttaacgaac cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc
    26101 gttctgttta accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgcttaggc
    26161 caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaatcaat
    26221 cagcaacttt atcaactact taacggcctg gatgtggttt accatgccca gggcgaatat
    26281 gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa
    26341 aatctcaccg caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg
    26401 ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca
    26461 catacacggg cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa
    26521 attcaccacg aatttaaccg ccacggtgcg cgctatcctt cctataacac cattgtaggc
    26581 agcggtgaaa acggctgcat tctgcactac accgaaaacg agagtgaact gcgcgacggt
    26641 gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga tattacccgc
    26701 accttcccgg tcaacggcaa attcacccag gcccagcgtg aaatctacga cattgtgctg
    26761 gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa cttccattct ggaagtcact
    26821 ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct gaaaggtaat
    26881 gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac
    26941 tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg
    27001 gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg
    27061 ccagaacaat atcgcggtat cggcattcgc attgaagacg acattgtgat taccgaaacc
    27121 ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg
    27181 gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc
    27241 tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga
    27301 ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg
    27361 gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg
    27421 ccatcactac cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag
    27481 aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc
    27541 tgtttgcatt gctgcgaaaa gcacctggcg taatgctgca ttgccccgat cgcgtagcta
    27601 acgttgcccg tactcagagt catgttgaag tgacgctgga gagcggcgag atgctgacgg
    27661 gccgcgtgct gatagccgct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg
    27721 attggcagca ggagccttac gaacaactgg cagtgattgc caacgttgct acttctgttg
    27781 cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga
    27841 tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc
    27901 tgtcgtggag cgacgagaag ttttgccgtg aactccagtc tgcctttggc tggcgactgg
    27961 ggcaaattac ccacgccggt aaacgcagtg cttatccgct ggcattaacc cgcgccgcca
    28021 gaccgattac ccatcgcact gtgctggtgg gcaatgcggc gcaaactctg cacccgattg
    28081 ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctgactc
    28141 aggcgcatga gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcagcagc
    28201 gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg
    28261 ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat
    28321 tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc
    28381 ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg
    28441 gggctggcgg ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag
    28501 cgcgtaccgg aacctctggc ggtagatgca ctaccacaac tgcgcgtttc ggctatcaat
    28561 gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct cgctcgtagg
    28621 gccagttgtt atcacggcat ggaagtgtgg gacaaagaca gttttggtca catctcgttt
    28681 gacgatcaaa gtatgggcta tagccatctt gggcacatcg ttgaaaactc agtgattcac
    28741 tacgcgctgt ggaacaaagc gcagcagtcg tcagatatta ctctgttggc ccccacagaa
    28801 ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga tggcagcatg
    28861 ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt cctggttgcg caacaaagca
    28921 gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg
    28981 gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt
    29041 ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc agaggaagcg
    29101 cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat
    29161 aatcgccttg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccgct gacggggcgt
    29221 tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc gcataccatt
    29281 cacccactgg cggggcaggg cgttaatctc ggatttatgg acgccgcaga gctgattgct
    29341 gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta cctgcgccgc
    29401 tatgaacgta gccgcaagca cagcgcggcg ctgatgctgg ctggtatgca gggttttcgc
    29461 gatctctttt ccggcgctaa tccggcgaaa aaactgctgc gtgatattgg tttgaaactg
    29521 gccgacacgc ttcctggcgt taagccgcaa cttatccgcc aggcaatggg attaaacgat
    29581 ttgcctgaat ggctgcgtta aaaatttctc ctctgttgtt tatttgatac ccatcacact
    29641 ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact aatttcacct
    29701 ccgttttcgc attatatttt ctaataccat gattttttga tttagtgttt tttgacattt
    29761 ttttagctct taatattgtc ttattcaaat tgactttctc atcacatcat ctttgtatag
    29821 aaattggtgt attttttggt tttttattct gtcgcgattt ttgcattttt taaccataag
    29881 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
    29941 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt tgtggtgata
    30001 atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
    30061 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
    30121 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc
    30181 ggaatgtttg atgtgtcgca tatgaccatc gtcgatctcc gcggcagccg cacccgggag
    30241 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagagg caaagccctt
    30301 tactcgggga tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt
    30361 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
    30421 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
    30481 attgccgtgc aagggccgaa tgctcaggca aaagctgcca cactgtttaa tgatgctcag
    30541 cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggtgggcga tctgtttatt
    30601 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
    30661 gccgatttct ggcgtgcgct ggtggaagcg ggcgttaagc cctgcggtct gggggcgcgt
    30721 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt
    30781 tctcctttag ccgccaacat gggctggact atcgcctggg aaccgacaga tcgtgacttt
    30841 atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact ggttggtctg
    30901 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac cgatgcgcag
    30961 ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct gggttacagc
    31021 attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
    31081 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
    31141 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
    31201 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
    31261 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
    31321 ttagcgcggg cgatgactgc gcggttgccg aatcggtaaa agcggcgtca gacatttatg
    31381 cgccagtaag cggtgaaatc gtggcagtaa acgacgcact gagcgattcc ccggaactgg
    31441 tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc gatgaaagcg
    31501 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagat gagtaacggc
    31561 tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc accctcactc
    31621 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    31681 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    31741 gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc
    31801 ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg cgcaccggcg
    31861 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    31921 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    31981 ctggaaaatc cgggctggta taccgcgtac actccgtatc agcctgaagt ctcccagggc
    32041 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg actggatatg
    32101 gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc gatggcgaaa
    32161 cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccaatgt gcatccgcaa
    32221 acgctggatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat tgtcgatgac
    32281 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact
    32341 accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg caaaattgtg
    32401 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    32461 gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca
    32521 cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg ccgtattatc
    32581 ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca gactcgcgag
    32641 caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    32701 aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg tatcgctaac
    32761 cgcattcacc gtctgaccga tattctggcg gcgggcctgc aacaaaaagg tctgaaactg
    32821 cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg
    32881 acgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc cgtggggatc
    32941 acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagtgt gctgctgggc
    33001 gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga cagccgctct
    33061 atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctat
    33121 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    33181 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    33241 atcccaatca cttggacgga atttgccgaa ctgcacccgt tttgcccgcc ggaacaggct
    33301 gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact gaccggttac
    33361 gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg cctgctggcg
    33421 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    33481 tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt ggtggttgtg
    33541 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    33601 ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt gtatgaagaa
    33661 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    33721 gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc ggacgtttcg
    33781 cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc gggtatggga
    33841 ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc
    33901 gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag cgcctctatc
    33961 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    34021 caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc cttcccggtg
    34081 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    34141 aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga ctacggtttc
    34201 cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    34261 gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg cgcagaaatt
    34321 gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa cgcgccgcac
    34381 attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcggtattc
    34441 ccggcaggcg tggcagacaa atactggccg acggtgaaac gtctggatga tgtttacggc
    34501 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt cactgattcg
    34561 agtattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac aaaagtgctc
    34621 agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccggcactgg
    34681 cgcgaatatt taccctcacc ccgaccctct ccctgaaagg gcgaggggga aaagcgtgcc
    34741 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    34801 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    34861 caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc
    34921 tccacgcggc gcaggaagtg atgaacttaa taatgcaagc cggtggcaaa gcattcgtgc
    34981 tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca atcgatcagc
    35041 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    35101 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    35161 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    35221 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa tatgttgatt
    35281 acgcggcatc gaaaggggcg attgattcgt taaccaccgg actatcgctg gaagtcgccg
    35341 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    35401 gcgcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccacc ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctgaca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtctatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 caca
//
LOCUS       DADLDT020000015        47285 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000015 DADLDT020000000
VERSION     DADLDT020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47285
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.015"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="JQH22_000819"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="JQH22_000819"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFB2205233.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="JQH22_000820"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="JQH22_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFB2205234.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="JQH22_000821"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="JQH22_000821"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFB2205235.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="JQH22_000822"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="JQH22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFB2205236.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="JQH22_000823"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="JQH22_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFB2205237.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="JQH22_000824"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="JQH22_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFB2205238.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="JQH22_000825"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="JQH22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFB2205239.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="JQH22_000826"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="JQH22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFB2205240.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="JQH22_000827"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="JQH22_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFB2205241.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="JQH22_000828"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="JQH22_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFB2205242.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="JQH22_000829"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="JQH22_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFB2205243.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="JQH22_000830"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="JQH22_000830"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFB2205244.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="JQH22_000831"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="JQH22_000831"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFB2205245.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="JQH22_000832"
     CDS             11233..12087
                     /locus_tag="JQH22_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFB2205246.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="JQH22_000833"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="JQH22_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFB2205247.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="JQH22_000834"
     CDS             complement(13550..14689)
                     /locus_tag="JQH22_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFB2205248.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="JQH22_000835"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="JQH22_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFB2205249.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="JQH22_000836"
     CDS             15875..17236
                     /locus_tag="JQH22_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HFB2205250.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="JQH22_000837"
     CDS             17233..17751
                     /locus_tag="JQH22_000837"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFB2205251.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="JQH22_000838"
     CDS             17751..18071
                     /locus_tag="JQH22_000838"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFB2205252.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="JQH22_000839"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="JQH22_000839"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFB2205253.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="JQH22_000840"
     CDS             18890..20092
                     /locus_tag="JQH22_000840"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFB2205254.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="JQH22_000841"
     CDS             20189..20611
                     /locus_tag="JQH22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFB2205255.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="JQH22_000842"
     CDS             complement(20659..21531)
                     /locus_tag="JQH22_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFB2205256.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="JQH22_000843"
     CDS             complement(21543..22637)
                     /locus_tag="JQH22_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFB2205257.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="JQH22_000844"
     CDS             complement(22670..23668)
                     /locus_tag="JQH22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2205258.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="JQH22_000845"
     CDS             complement(23693..25204)
                     /locus_tag="JQH22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFB2205259.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="JQH22_000846"
     CDS             complement(25227..26210)
                     /locus_tag="JQH22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFB2205260.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="JQH22_000847"
     CDS             complement(26307..29408)
                     /locus_tag="JQH22_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFB2205261.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="JQH22_000848"
     CDS             complement(29415..29588)
                     /locus_tag="JQH22_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205262.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30889
                     /locus_tag="JQH22_000849"
                     /pseudo
     CDS             29706..30889
                     /locus_tag="JQH22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(31087..32340)
                     /gene="glyA"
                     /locus_tag="JQH22_000850"
     CDS             complement(31087..32340)
                     /gene="glyA"
                     /locus_tag="JQH22_000850"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFB2205263.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32667..33857
                     /gene="hmpA"
                     /locus_tag="JQH22_000851"
     CDS             32667..33857
                     /gene="hmpA"
                     /locus_tag="JQH22_000851"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFB2205264.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33902..34240)
                     /gene="glnB"
                     /locus_tag="JQH22_000852"
     CDS             complement(33902..34240)
                     /gene="glnB"
                     /locus_tag="JQH22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFB2205265.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34301..35635)
                     /gene="glrR"
                     /locus_tag="JQH22_000853"
     CDS             complement(34301..35635)
                     /gene="glrR"
                     /locus_tag="JQH22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFB2205266.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35625..36338)
                     /gene="qseG"
                     /locus_tag="JQH22_000854"
     CDS             complement(35625..36338)
                     /gene="qseG"
                     /locus_tag="JQH22_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFB2205267.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36503..37930)
                     /gene="qseE"
                     /locus_tag="JQH22_000855"
     CDS             complement(36503..37930)
                     /gene="qseE"
                     /locus_tag="JQH22_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFB2205268.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38506..42393)
                     /gene="purL"
                     /locus_tag="JQH22_000856"
                     /gene_synonym="purI"
     CDS             complement(38506..42393)
                     /gene="purL"
                     /locus_tag="JQH22_000856"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFB2205269.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42652..44208
                     /gene="mltF"
                     /locus_tag="JQH22_000857"
     CDS             42652..44208
                     /gene="mltF"
                     /locus_tag="JQH22_000857"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFB2205270.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44205..44741)
                     /gene="tadA"
                     /locus_tag="JQH22_000858"
     CDS             complement(44205..44741)
                     /gene="tadA"
                     /locus_tag="JQH22_000858"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFB2205271.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44766..45401)
                     /gene="yfhb"
                     /locus_tag="JQH22_000859"
     CDS             complement(44766..45401)
                     /gene="yfhb"
                     /locus_tag="JQH22_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFB2205272.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45610..46458
                     /locus_tag="JQH22_000860"
     CDS             45610..46458
                     /locus_tag="JQH22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFB2205273.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46797..47285)
                     /locus_tag="JQH22_000861"
                     /pseudo
     CDS             complement(46797..47285)
                     /locus_tag="JQH22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11274 a        12954 c        11887 g        11170 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaggcat
    30241 tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat ggcaaaataa
    30301 ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct cgtcgttcgt
    30361 gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg gtcacaccat
    30421 cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg aaaccgtcgc
    30481 ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac cggttaatac
    30541 tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc agagtggaga
    30601 accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga gtctgtataa
    30661 cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt gtgcctttgg
    30721 tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg accgcgacga
    30781 aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg cacaacaggt
    30841 gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat ggcgcgataa
    30901 cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg cctgatgcgc
    30961 tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat tttgtaggcc
    31021 tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag caaatcaccg
    31081 tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac ctttgatgcg
    31141 ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc cagccagttc
    31201 tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta cacgaatacc
    31261 ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga cggtgatgtt
    31321 agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa ccagatcaac
    31381 caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct cgaggaacac
    31441 ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga actcaggctc
    31501 catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg gaccgccctg
    31561 accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac cacctttcgc
    31621 caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag taacaacgtg
    31681 agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa cgtgcgccat
    31741 atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg cccagtcaac
    31801 cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt ctttggcttg
    31861 tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag gaacgatgtt
    31921 gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc catgcgccag
    31981 gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg caaagttagc
    32041 ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt ctttcgcacg
    32101 atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt agcgtttgcc
    32161 cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca ttacgcgcgg
    32221 gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta ctttttcctg
    32281 ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac gctttaacat
    32341 ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg tcctgttgga
    32401 caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa aggaatttac
    32461 gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg caaccggaaa
    32521 cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct ttctgaaaaa
    32581 caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg agatacatca
    32641 attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct acagtaaaag
    32701 ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc tacgaccgta
    32761 tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg
    32821 atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt gaaaacctgc
    32881 ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc cagatcaaac
    32941 cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa atgttcagcc
    33001 cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct aatgtattta
    33061 tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt tgggaaggta
    33121 ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc agcttcgaac
    33181 tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat ctcggcgtct
    33241 ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg actcgcaaac
    33301 cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag gtatccaact
    33361 ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt
    33421 tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt gttggtcaaa
    33481 cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca caagtgaact
    33541 ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt aaggaactgg
    33601 ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc gaagccgatc
    33661 gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt
    33721 tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg cagtttaccg
    33781 cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa tgctttggcc
    33841 cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc cttgacgcgg
    33901 tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga gccacgtcaa
    33961 agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata atggtatcga
    34021 cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc ggcagaaaat
    34081 ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga ccaaagcctt
    34141 tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg cggacatcgt
    34201 ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt gaaaaggttg
    34261 cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg aaatcgtttg
    34321 catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc cccgccattc
    34381 tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa tagttgagtt
    34441 caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc tccagcgcct
    34501 gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac tgttcaatca
    34561 cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt ttcatcgcat
    34621 cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc aacaggtgat
    34681 ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc aggctgacaa
    34741 cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct tttgacagat
    34801 cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta ctgcccagcg
    34861 ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc gcaggcatat
    34921 cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa ccttcgcgat
    34981 tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac tccagcaatt
    35041 gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc gggctggcgt
    35101 tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta atcaaaacgc
    35161 tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc agcgggctgc
    35221 gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc tccagcgcat
    35281 cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa ctaaaaacgc
    35341 cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga ataattactg
    35401 gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc atttcatcca
    35461 tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt aatccttcag
    35521 cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg ccaagcagtt
    35581 tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg ctcatcagga
    35641 gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc cggcgagaaa
    35701 tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt ttccagcttg
    35761 cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa tgtatccagc
    35821 tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc cagttgcagt
    35881 tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc cgggatctcg
    35941 gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt aattttggcg
    36001 tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc atattggcga
    36061 gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc ccgcagccag
    36121 taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct acattcagta
    36181 gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt atcaatggct
    36241 ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc tgccagccac
    36301 agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact taaagcctat
    36361 cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga aaccggggcg
    36421 tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa ataaaatagc
    36481 ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat tcaatgcgga
    36541 aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata cggcgaatac
    36601 aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt cgctggtggc
    36661 ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg
    36721 tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg caaatgttac
    36781 cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg ctcatcagca
    36841 gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc atcattttag
    36901 cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct aattcaacat
    36961 tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt tgttcgatca
    37021 gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt tgctctggcg
    37081 taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata ctcgccagtg
    37141 gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat tccagccatg
    37201 acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg ctcggtccac
    37261 agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc atgcgctcga
    37321 tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc agactcacca
    37381 gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt tcacgctgaa
    37441 gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg gtattggcac
    37501 tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg ttacactgaa
    37561 gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg tctggcagta
    37621 cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga taaaccttcg
    37681 caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc tccagcgccg
    37741 cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta accagcgccg
    37801 cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc aacaggggta
    37861 gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga aaaacgggcc
    37921 agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc gaggggaagt
    37981 tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg gtgcctaact
    38041 cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga cggcaggcac
    38101 cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac ataagtcggt
    38161 gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat agtttggtaa
    38221 cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg aatgtaaatc
    38281 agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta caataaaatc
    38341 aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt gatttacaga
    38401 cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa tgccggatgc
    38461 ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc ccaactgctt
    38521 acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt ccggatgcca
    38581 ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg tgactcgacc
    38641 actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag ccgggtaggt
    38701 ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt tgctttccag
    38761 tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag agacagcaat
    38821 cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg ttacttcaac
    38881 caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg gccacaactc
    38941 actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac atacccccag
    39001 ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac ggtcattgaa
    39061 caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg agaaacctcc
    39121 gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca ggtcgctcat
    39181 atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt caacatgcga
    39241 gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg caatatacgg
    39301 tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg gatcggcgtc
    39361 gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca ggcgctgcat
    39421 ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc tgaatacagt
    39481 ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa cataatggac
    39541 gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt cagcggcacg
    39601 aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat cgtcacccag
    39661 agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg ccagcgttac
    39721 cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg caaccagcgc
    39781 ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg ccggtttgtc
    39841 gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat tgcctttgcc
    39901 caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga tggtgtgacg
    39961 tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg tcatttcgcg
    40021 ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac ccaccgggat
    40081 cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt catacaggcc
    40141 cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt taatgcgttt
    40201 gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg ccagacgggc
    40261 agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca tcgcttcacc
    40321 gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca cctgccacgg
    40381 ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga tggtcaccag
    40441 gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt ctgcaatggt
    40501 gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat cgcgggtcat
    40561 cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat tatcaaaatg
    40621 acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg cgtagggtgc
    40681 gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa ccgccagcac
    40741 gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg gttcgtcgct
    40801 tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca gctccggcat
    40861 cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt tagcatcacc
    40921 aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg ggttgtcgcg
    40981 ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg aagacgccgc
    41041 gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct tcgcaccgac
    41101 gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc ccgccagcat
    41161 gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt tttcttcata
    41221 agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg ccgcaccgcc
    41281 cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct taccgaaatc
    41341 ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga aaccaaccaa
    41401 accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt caccgccgga
    41461 accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag tttcgacttt
    41521 catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt ggtcagcaaa
    41581 gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag cagagagaac
    41641 gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt tcggctgttg
    41701 ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct cagagttcgc
    41761 ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg tgaaagcatc
    41821 ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt tagcgtcgat
    41881 cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg gttgatggtg
    41941 ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca tgcggtcgtg
    42001 cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt ctatatagta
    42061 agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg caatatcggt
    42121 cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca ggagtttgcc
    42181 ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa gttgtgcgtg
    42241 ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg cgtaaatatt
    42301 gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc ggaatgccga
    42361 cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta agctttcggt
    42421 gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt
    42481 ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat ggctgtaata
    42541 agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa acctttcact
    42601 attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta attgaaaaaa
    42661 ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt cgctctctgg
    42721 ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca agcgcgggga
    42781 gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa cgggaaacct
    42841 tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa
    42901 gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg taacgccgac
    42961 ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca gcctggccct
    43021 acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg cccacgtacg
    43081 ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat
    43141 ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa
    43201 ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac cattgctgat
    43261 tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc
    43321 accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac cctttccgcc
    43381 gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg cattgaagag
    43441 aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt tttacgcgcc
    43501 gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga agaaattgac
    43561 tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca ggccacttca
    43621 ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag cctcggcatt
    43681 acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt gcaggatatg
    43741 atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc cctcgctgcg
    43801 tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa aaccaaaggg
    43861 aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca gaaaccctat
    43921 tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta tgtcgaaaat
    43981 attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa gcaggctaca
    44041 gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga gttgggcaaa
    44101 gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt gacccattct
    44161 ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc cgtcgaggat
    44221 tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc actgagcaac
    44281 gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt catacccgga
    44341 tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg cgcaccaaag
    44401 accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca tggttcaagc
    44461 gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc accctgccgc
    44521 agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg gttccagcct
    44581 tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc ccgctcatcc
    44641 caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct aaattcgact
    44701 tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg acgctatgct
    44761 ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg atgctgacag
    44821 aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag ccgcagcggc
    44881 gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg catcgtcaat
    44941 acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg cggcagccag
    45001 ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat caaccagata
    45061 tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta
    45121 acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg tgcttcgctg
    45181 tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc acgacctttt
    45241 atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc attcagcggt
    45301 tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg caatgttcca
    45361 tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc ctctggactg
    45421 aaacatcctg attaattttc gctactacat agcctaacag atagatcatc acttttccgg
    45481 caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt agcggcaatg
    45541 gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta tttcatcgcg
    45601 aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct tgcccagagt
    45661 gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca tttaagctct
    45721 cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa
    45781 ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag
    45841 ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc gttacggctg
    45901 gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct aaacgttaat
    45961 agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg gataattctg
    46021 accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct gatgaagatt
    46081 ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt acaggcgtcg
    46141 tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga gttaaacctg
    46201 gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg ctttactccg
    46261 aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga acaagcgaca
    46321 aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt gctgttcatt
    46381 gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag tgaagcgctg
    46441 gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt
    46501 tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg atatgtgtga
    46561 aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt gaatgtgttg
    46621 ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa caccactttc
    46681 tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc tgtgacatac
    46741 aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct gcggttttat
    46801 ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata gatgatcgcc
    46861 accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca ttattctggt
    46921 gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc cagtcctgct
    46981 tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat cacatggaag
    47041 aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg aaagttgatg
    47101 cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg ccccagccga
    47161 tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag ttttttcagc
    47221 cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc gcatcctgca
    47281 cagtt
//
LOCUS       DADLDT020000016        46409 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000016 DADLDT020000000
VERSION     DADLDT020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 46409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46409
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.016"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            99..164
                     /locus_tag="JQH22_000862"
     CDS             99..164
                     /locus_tag="JQH22_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205274.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            157..501
                     /gene="erpA"
                     /locus_tag="JQH22_000863"
     CDS             157..501
                     /gene="erpA"
                     /locus_tag="JQH22_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFB2205275.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(548..1171)
                     /locus_tag="JQH22_000864"
     CDS             complement(548..1171)
                     /locus_tag="JQH22_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFB2205276.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(1209..2009)
                     /gene="btuF"
                     /locus_tag="JQH22_000865"
     CDS             complement(1209..2009)
                     /gene="btuF"
                     /locus_tag="JQH22_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFB2205277.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(2002..2700)
                     /gene="mtnN"
                     /locus_tag="JQH22_000866"
     CDS             complement(2002..2700)
                     /gene="mtnN"
                     /locus_tag="JQH22_000866"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFB2205278.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            2784..4301
                     /gene="dgt"
                     /locus_tag="JQH22_000867"
     CDS             2784..4301
                     /gene="dgt"
                     /locus_tag="JQH22_000867"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFB2205279.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            4431..5855
                     /gene="degP"
                     /locus_tag="JQH22_000868"
     CDS             4431..5855
                     /gene="degP"
                     /locus_tag="JQH22_000868"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFB2205280.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            6010..7166
                     /gene="cdaR"
                     /locus_tag="JQH22_000869"
                     /pseudo
     CDS             6010..7166
                     /gene="cdaR"
                     /locus_tag="JQH22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(7255..7641)
                     /locus_tag="JQH22_000870"
     CDS             complement(7255..7641)
                     /locus_tag="JQH22_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFB2205281.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(7955..8779)
                     /gene="dapD"
                     /locus_tag="JQH22_000871"
     CDS             complement(7955..8779)
                     /gene="dapD"
                     /locus_tag="JQH22_000871"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFB2205282.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(8810..11482)
                     /gene="glnD"
                     /locus_tag="JQH22_000872"
     CDS             complement(8810..11482)
                     /gene="glnD"
                     /locus_tag="JQH22_000872"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFB2205283.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(11544..12338)
                     /gene="map"
                     /locus_tag="JQH22_000873"
     CDS             complement(11544..12338)
                     /gene="map"
                     /locus_tag="JQH22_000873"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFB2205284.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            12706..13431
                     /gene="rpsB"
                     /locus_tag="JQH22_000874"
     CDS             12706..13431
                     /gene="rpsB"
                     /locus_tag="JQH22_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFB2205285.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            13689..14540
                     /gene="tsf"
                     /locus_tag="JQH22_000875"
     CDS             13689..14540
                     /gene="tsf"
                     /locus_tag="JQH22_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HFB2205286.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            14687..15412
                     /gene="pyrH"
                     /locus_tag="JQH22_000876"
     CDS             14687..15412
                     /gene="pyrH"
                     /locus_tag="JQH22_000876"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFB2205287.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            15704..16261
                     /gene="frr"
                     /locus_tag="JQH22_000877"
     CDS             15704..16261
                     /gene="frr"
                     /locus_tag="JQH22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFB2205288.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            16353..17549
                     /gene="ispC"
                     /locus_tag="JQH22_000878"
     CDS             16353..17549
                     /gene="ispC"
                     /locus_tag="JQH22_000878"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFB2205289.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            17738..18496
                     /gene="ispU"
                     /locus_tag="JQH22_000879"
                     /gene_synonym="uppS"
     CDS             17738..18496
                     /gene="ispU"
                     /locus_tag="JQH22_000879"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFB2205290.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            18509..19366
                     /gene="cdsA"
                     /locus_tag="JQH22_000880"
     CDS             18509..19366
                     /gene="cdsA"
                     /locus_tag="JQH22_000880"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFB2205291.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            19378..20730
                     /gene="rseP"
                     /locus_tag="JQH22_000881"
     CDS             19378..20730
                     /gene="rseP"
                     /locus_tag="JQH22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFB2205292.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            20760..23192
                     /gene="bamA"
                     /locus_tag="JQH22_000882"
     CDS             20760..23192
                     /gene="bamA"
                     /locus_tag="JQH22_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFB2205293.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            23314..23799
                     /gene="skp"
                     /locus_tag="JQH22_000883"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             23314..23799
                     /gene="skp"
                     /locus_tag="JQH22_000883"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFB2205294.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            23803..24828
                     /gene="lpxD"
                     /locus_tag="JQH22_000884"
     CDS             23803..24828
                     /gene="lpxD"
                     /locus_tag="JQH22_000884"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFB2205295.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            24933..25388
                     /gene="fabZ"
                     /locus_tag="JQH22_000885"
     CDS             24933..25388
                     /gene="fabZ"
                     /locus_tag="JQH22_000885"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFB2205296.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            25392..26180
                     /gene="lpxA"
                     /locus_tag="JQH22_000886"
     CDS             25392..26180
                     /gene="lpxA"
                     /locus_tag="JQH22_000886"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFB2205297.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            26180..27328
                     /gene="lpxB"
                     /locus_tag="JQH22_000887"
     CDS             26180..27328
                     /gene="lpxB"
                     /locus_tag="JQH22_000887"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFB2205298.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            27325..27921
                     /gene="rnhB"
                     /locus_tag="JQH22_000888"
     CDS             27325..27921
                     /gene="rnhB"
                     /locus_tag="JQH22_000888"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFB2205299.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            27958..31440
                     /gene="dnaE"
                     /locus_tag="JQH22_000889"
     CDS             27958..31440
                     /gene="dnaE"
                     /locus_tag="JQH22_000889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFB2205300.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            31453..32412
                     /gene="accA"
                     /locus_tag="JQH22_000890"
     CDS             31453..32412
                     /gene="accA"
                     /locus_tag="JQH22_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFB2205301.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            32511..34652
                     /gene="ldcC"
                     /locus_tag="JQH22_000891"
     CDS             32511..34652
                     /gene="ldcC"
                     /locus_tag="JQH22_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFB2205302.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCPV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            34709..35098
                     /locus_tag="JQH22_000892"
     CDS             34709..35098
                     /locus_tag="JQH22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFB2205303.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            35163..36461
                     /gene="tilS"
                     /locus_tag="JQH22_000893"
     CDS             35163..36461
                     /gene="tilS"
                     /locus_tag="JQH22_000893"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFB2205304.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLKLPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(36510..36770)
                     /gene="rof"
                     /locus_tag="JQH22_000894"
     CDS             complement(36510..36770)
                     /gene="rof"
                     /locus_tag="JQH22_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFB2205305.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(36757..36957)
                     /locus_tag="JQH22_000895"
     CDS             complement(36757..36957)
                     /locus_tag="JQH22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFB2205306.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            37123..37668
                     /locus_tag="JQH22_000896"
     CDS             37123..37668
                     /locus_tag="JQH22_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFB2205307.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            37665..38087
                     /gene="arfB"
                     /locus_tag="JQH22_000897"
     CDS             37665..38087
                     /gene="arfB"
                     /locus_tag="JQH22_000897"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFB2205308.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            38101..38811
                     /gene="nlpE"
                     /locus_tag="JQH22_000898"
                     /gene_synonym="cutF"
     CDS             38101..38811
                     /gene="nlpE"
                     /locus_tag="JQH22_000898"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFB2205309.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(38966..39790)
                     /locus_tag="JQH22_000899"
     CDS             complement(38966..39790)
                     /locus_tag="JQH22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFB2205310.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(39843..41561)
                     /gene="proS"
                     /locus_tag="JQH22_000900"
     CDS             complement(39843..41561)
                     /gene="proS"
                     /locus_tag="JQH22_000900"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFB2205311.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(41672..42379)
                     /gene="tsaA"
                     /locus_tag="JQH22_000901"
     CDS             complement(41672..42379)
                     /gene="tsaA"
                     /locus_tag="JQH22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFB2205312.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(42376..42780)
                     /gene="rcsF"
                     /locus_tag="JQH22_000902"
     CDS             complement(42376..42780)
                     /gene="rcsF"
                     /locus_tag="JQH22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFB2205313.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(42898..43713)
                     /gene="metQ"
                     /locus_tag="JQH22_000903"
     CDS             complement(42898..43713)
                     /gene="metQ"
                     /locus_tag="JQH22_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFB2205314.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(43753..44406)
                     /gene="metI"
                     /locus_tag="JQH22_000904"
     CDS             complement(43753..44406)
                     /gene="metI"
                     /locus_tag="JQH22_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFB2205315.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(44399..45430)
                     /gene="metN"
                     /locus_tag="JQH22_000905"
     CDS             complement(44399..45430)
                     /gene="metN"
                     /locus_tag="JQH22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFB2205316.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            45618..46193
                     /gene="gmhB"
                     /locus_tag="JQH22_000906"
     CDS             45618..46193
                     /gene="gmhB"
                     /locus_tag="JQH22_000906"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFB2205317.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        11297 a        11366 c        12598 g        11148 t
ORIGIN      
        1 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
       61 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
      121 aatttgcccg attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
      181 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
      241 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
      301 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
      361 ctggtggttg atccgatgag cctgcaatat ctggtcggcg gttccgttga ctacaccgaa
      421 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
      481 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
      541 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
      601 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
      661 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
      721 cgtagatttc tgtacgtaaa atcatgggga tttcgcggac cagtacatca cgaatgatcc
      781 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
      841 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
      901 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
      961 tgaccatggc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
     1021 gcgccatgtc gcgaattgtt ccgtcgccta ctgcggtaac cacgcccagt accagaacac
     1081 caaaagggtc catacgcaat tttccggcta acaaaacgcc gtagatggca aataccgctg
     1141 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag
     1201 gtcaggcact aatctacctg tgaaagcgca ttacagagct gttgtgctgc gaggataata
     1261 cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
     1321 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatagcc
     1381 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct
     1441 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca
     1501 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
     1561 tattgcgctt tcaattgcac gtactgatcc agcagggatt gcgcggcttg ttcggccttg
     1621 tctggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
     1681 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
     1741 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
     1801 ttcatcccct gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa
     1861 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
     1921 gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg gcgcgagaaa agacagggcg
     1981 accagcgccc tgaacagtga tttagccatg tgccagtttc tgcaccagtg actcaaccat
     2041 caggctggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
     2101 atcggccacg tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac
     2161 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
     2221 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
     2281 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
     2341 tttaaagcct gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc
     2401 cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc
     2461 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcag
     2521 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
     2581 tccattcagt tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc
     2641 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
     2701 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
     2761 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
     2821 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg
     2881 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
     2941 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
     3001 tccagcaggt gggacgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat
     3061 tgctggaagc atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt
     3121 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcagcgataa
     3181 atgactggtt tcgccaacgt ttgcacccgg aagatgccga aagccagcct ctgactgacg
     3241 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc
     3301 ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg
     3361 tgcatacatt gatgcggatg aatctcactt gggcacaggt tggcggtatt ttaaaatata
     3421 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
     3481 cgggttatta tctttctgaa gaagcctata ttgcccgctt gcgtaaagaa cttaatttgg
     3541 cgctttacag tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt
     3601 gtgtggcaga ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc
     3661 atcatttgca cgaagcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg
     3721 aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt
     3781 ttatgtattt acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta
     3841 ttgataatct gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca
     3901 gcgaatgcag cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc
     3961 atccggatgt cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga
     4021 tttatcgtcc tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac
     4081 gggtgaaacg tttccctatt gagtcgcgct tattccacaa actctcgacg cgccatcggc
     4141 tggcctatgt cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg
     4201 aatattatta ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg
     4261 cgtgggatga ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg
     4321 aacaataaat ttttaccttt tgcagaaact ttagttcgga acttcgggct ataaaacgaa
     4381 tctgaagaac acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa
     4441 ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg
     4501 caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga
     4561 tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta
     4621 atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc
     4681 aggaaggttc tccgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg
     4741 gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag
     4801 gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctga
     4861 gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc
     4921 tgatccaaat ccagaacccg aaaaaactga ccgcaattaa gatggcggat tctgatgcac
     4981 tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc gagacggtaa
     5041 cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact
     5101 tcatccagac cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga
     5161 acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta
     5221 tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg gtggaatacg
     5281 gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga
     5341 aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct
     5401 ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccga
     5461 tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga
     5521 ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg cagcagagca
     5581 gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca
     5641 acaaaggcaa agatcagggc gtggtagtga acaacgtgaa gacgggcact ccggctgcgc
     5701 agatcggcct gaagaaaggt gatgtgatta ttggtgcgaa ccagcaggca gtgaaaaaca
     5761 tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc
     5821 gcggcgacag caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaatac
     5881 gggaaggggt tctccttaca atctgtgaac ttcgccacaa ctccatacat cttcatcatc
     5941 ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac
     6001 ggaggactta tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt
     6061 accatgcgca tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc
     6121 agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag
     6181 ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc tgcacggtgt gcggcagggg
     6241 attaatctac cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa
     6301 ccagagaatc tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa
     6361 cagtcgcggt tgatgcactt gttggcgcag gatagccgtt tgcgggaaga actggtgatg
     6421 aacctgattc aggcagagga gaatactccc gcacttctga atgggcgcaa cggctgggga
     6481 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg
     6541 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
     6601 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
     6661 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
     6721 tgaaagagta cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg
     6781 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
     6841 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
     6901 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
     6961 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc
     7021 tggcaacgtc aaaggcgttg tttattcatc gtaataccct ggagtatcgg cttaatcgta
     7081 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
     7141 cgttacaact ggatgaagag cggtagatta tgcgttaagg ttgaggcagc gctggctcat
     7201 tcccctcagc ccgaagggga gaggggaatg cccgagttaa ccgtcaattc gggattattt
     7261 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
     7321 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
     7381 acagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
     7441 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
     7501 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
     7561 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
     7621 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
     7681 tagcatttgg cttggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
     7741 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat attccatggg
     7801 atggattagc acatttatct tcgttttgcg aattacatga cagtccgaaa caagatgaaa
     7861 aagtatttaa aaaaggggga ggaagaatga gtaggacgaa ttattctgag ccagaaagca
     7921 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
     7981 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
     8041 ggctgtattt gccatctttt gacggcagat tacctgaaac aaccacagac cccgccggaa
     8101 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
     8161 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcagagc
     8221 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
     8281 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
     8341 cgacggtcgc ccaggtatca accatggtgc cttcatcaac atatgcgccg atgttaacgt
     8401 aagacggcat cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc gccgctggtg
     8461 gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg aatttcatcg
     8521 gcactttgtc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
     8581 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
     8641 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
     8701 taacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
     8761 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
     8821 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
     8881 gcgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
     8941 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actcgcgcca
     9001 gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg
     9061 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
     9121 gctgcggtgg ctgccagcta ctttgcgtca gcacctgctc cagaccaaac cgaataacct
     9181 catggcgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
     9241 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
     9301 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
     9361 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
     9421 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
     9481 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
     9541 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
     9601 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
     9661 tattccacag tgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
     9721 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
     9781 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
     9841 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
     9901 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
     9961 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    10021 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    10081 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    10141 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag tccgcgccgc accgctccgg
    10201 ggtgacgcag aatgctcaaa aacagttttc gtgcttgcgg aatattacac agcggttgtt
    10261 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    10321 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    10381 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgagtggac
    10441 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    10501 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    10561 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    10621 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    10681 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    10741 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    10801 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    10861 aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc gtagaacttg tcggaaggcc
    10921 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    10981 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccaacatgc
    11041 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    11101 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    11161 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcaaccaatg
    11221 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcta
    11281 tgaactcggt acgtgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    11341 ccagccaacg ctggaaagta tcgatatggg cttttatccc acagacggtt aattcatcac
    11401 ggggccaggc gcatggattt tgcggttgac cgggcagggt ggagagagcg gtgtttgcgt
    11461 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    11521 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    11581 tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    11641 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    11701 tcttttttac ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg
    11761 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    11821 ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaatttctga
    11881 atcgccgcac cgatttcgcg cagattaatg cctggtttta ccatgcgtag cgccaggtac
    11941 aggctttctt gcgtgatgcg gcacaggcgt tcgcccatga tggtcggctt accgacgata
    12001 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    12061 atatcgccat ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta
    12121 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    12181 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    12241 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    12301 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    12361 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    12421 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacgtc
    12481 tgatgctgta cacagcgcca acaattattg gtgtccacga cgtatttgtg gtataaagcg
    12541 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    12601 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    12661 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    12721 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    12781 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    12841 actgtaccga tgttcaacga agctctggct gaactgaaca agattgtttc tcgcaaaggt
    12901 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    12961 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    13021 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    13081 ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    13141 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    13201 gctgaccacg aacacattgc tatcaaagaa gcaaacaact tgggtattcc ggtatttgct
    13261 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    13321 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    13381 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    13441 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    13501 cttacatcag taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata
    13561 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    13621 gccccctttt tcacttttat atctgtgtgg tttaatgccg ggcagatcac atctccgagg
    13681 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    13741 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    13801 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    13861 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    13921 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    13981 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    14041 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    14101 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    14161 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    14221 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    14281 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    14341 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    14401 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    14461 gtttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    14521 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    14581 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    14641 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    14701 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    14761 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    14821 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    14881 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    14941 aatgaacggc ttggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    15001 gtccgctatt ccattgaatg gcgtgtgcga tagctacagc tgggcagaag ctatcagcct
    15061 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    15121 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    15181 caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat tcaaccgcaa ccatgtacga
    15241 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    15301 cacgctggct cgtgaccata aattaccgat tcgtgttttc aatatgaaca aaccgggtgc
    15361 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    15421 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag
    15481 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacaaaag
    15541 tacgtgagga tggcgagcac tgctcggggc caaaatggca aataaaatag cctaataatc
    15601 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    15661 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    15721 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    15781 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    15841 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    15901 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcctccga
    15961 tctcggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    16021 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    16081 tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    16141 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    16201 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    16261 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    16321 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    16381 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    16441 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    16501 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    16561 agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    16621 cgcttgagga ggttgatcag gtgatggcag ccattgttgg tgctgctggg ctgttaccta
    16681 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    16741 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    16801 atagcgaaca taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    16861 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    16921 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagct tgccgtcatc
    16981 cgaactggtc gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    17041 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    17101 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    17161 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    17221 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    17281 cggattatga gcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg
    17341 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    17401 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    17461 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgagt gaagtcgcca
    17521 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    17581 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    17641 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    17701 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    17761 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    17821 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    17881 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    17941 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    18001 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    18061 tattggcgat accagtcgtt ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    18121 gctaacagcc ggaaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    18181 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    18241 agatcagata gatgaagaga tgttaaacca gcatgtctgt atgcatgaac tggcccctgt
    18301 agatttagta attaggactg ggggagagca tcgcattagt aactttttgc tttggcaaat
    18361 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    18421 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    18481 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    18541 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    18601 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    18661 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    18721 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    18781 ttcgttgggt tggtggattg tcgcgctatt gctggtgctg ttttaccctg gttccgcagc
    18841 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    18901 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    18961 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    19021 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    19081 aggctttata ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc
    19141 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    19201 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    19261 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    19321 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    19381 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    19441 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    19501 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    19561 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    19621 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    19681 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    19741 attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgcg
    19801 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    19861 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    19921 gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    19981 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    20041 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    20101 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    20161 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt
    20221 cccttgtctt tgacattaat cccggggagt aaaccgggta atggtaaagc gattggtttt
    20281 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    20341 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    20401 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    20461 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    20521 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    20581 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    20641 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    20701 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    20761 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    20821 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    20881 cggccctcct cagtatgccg gtgcgtacag gcgacacggt taatgatgaa gatatcagta
    20941 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    21001 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    21061 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    21121 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    21181 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    21241 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    21301 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    21361 acgaagtgcc gtggtggaat gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    21421 gcgatcttga aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatcg
    21481 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    21541 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    21601 ccgctgaaat tgagcagctg actaagatcg agccgggcga gctgtataac ggcactaaag
    21661 tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg ctatggttat gcctatccgc
    21721 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    21781 ttgatgtggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    21841 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggttg gggagcgatc
    21901 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    21961 atacccaacg tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac
    22021 gtaacactgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    22081 aggctggcgt gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    22141 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    22201 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    22261 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgattaacg
    22321 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    22381 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    22441 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    22501 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    22561 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    22621 tcgatgacga tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt
    22681 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    22741 gtggcttcca gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta
    22801 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    22861 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    22921 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttcattc ttctgggata
    22981 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    23041 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    23101 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    23161 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    23221 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    23281 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    23341 cttggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg
    23401 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag
    23461 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    23521 aaactgcagt ccatgaaagc gggcagcgat cgcaccaaac tggaaaaaga cgtgatggct
    23581 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    23641 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    23701 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    23761 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    23821 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    23881 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    23941 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    24001 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    24061 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc
    24121 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    24181 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    24241 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    24301 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    24361 aacgatcgtg gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc
    24421 gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc
    24481 aatggcgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    24541 acggcggttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    24601 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    24661 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    24721 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    24781 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    24841 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    24901 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    24961 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    25021 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    25081 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    25141 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    25201 tgtactactt cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc
    25261 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    25321 ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    25381 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    25441 gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg
    25501 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    25561 gccgcgataa tgagatttat cagtttgcct ccatcggcga agttaaccag gatttgaaat
    25621 atgctggcga accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    25681 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    25741 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca
    25801 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    25861 ccgcagtcca tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg
    25921 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    25981 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    26041 atgcgtataa gctaatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    26101 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    26161 cgcgcggtct gattcgttaa tgactgacca gcgtccatta acgattgccc tggtcgccgg
    26221 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    26281 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    26341 gtacgaaatg gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    26401 cttactgcat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt
    26461 tgttggtatt gatgcgcccg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    26521 tatcaaaacc attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt
    26581 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    26641 tgacaaatac aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt
    26701 agatccagat aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct
    26761 ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcttgaa
    26821 aactgcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa
    26881 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctgtcggt
    26941 tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc agcgatgcgg cactactggc
    27001 gtcggggacg gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg
    27061 catgaagcct tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct
    27121 gccaaatctg ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc
    27181 gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat
    27241 gcacgatacc ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    27301 acaagccgtt ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt
    27361 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    27421 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    27481 cgccgtctgg cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    27541 gcggaacccc acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag
    27601 cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    27661 ccgaaattac cgatgcctgc gatggctgtg gtgaaagggg atagccgcgt accagaaatc
    27721 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    27781 gttttcccgc aatatggttt tgcccaacat aaagggtatc caaccgcttt tcatctggaa
    27841 aaactggctg aatatggcgc gactgaacat catcgtcgca gctttgggcc tgtcaaacgc
    27901 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    27961 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcttg
    28021 gccaaaaccg caccgctggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc
    28081 accgatttca ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg
    28141 attaagccta tcgtcggggc agattttaat gtccagtgcg acctgctggg tgatgagtta
    28201 acccacctga cggtactggc ggcgaacaat accggctatc agaatctggc gttgctgatc
    28261 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    28321 atcgaattaa acgaagggtt gatccttctt tccggcgggc gcatgggcga cgtcggacgc
    28381 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    28441 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat
    28501 ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat
    28561 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    28621 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    28681 agcgaagagg agatgtgtga gctgtttgcc gacatcccag aagcccttgc caacaccgtt
    28741 gagatcgcca aacgctgtaa cgtaactatg cgtcttggtg aatacttcct gccgcagttc
    28801 ccgaccgggg acatgagcac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa
    28861 gagcgtctgg catttttatt ccctgacgag gaagaacgtc ttaaacgccg cccggaatat
    28921 gacgaacgtc tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc
    28981 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc
    29041 cgtggctccg gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg
    29101 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    29161 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcggacatg
    29221 tacggtcgtg atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg
    29281 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgaccg tatctcgaaa
    29341 ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    29401 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    29461 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    29521 attaccgatt ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt
    29581 gataaaagcg acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg
    29641 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    29701 ccaccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    29761 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    29821 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccctggt
    29881 ccgttgcagt cagggatggt ggataacttt atcgaccgta aacatggtcg tgaagagatc
    29941 tcctatccgg acgtgcagtg gcagcatgaa agcctgaaat cggtactgga gccaacctac
    30001 ggcattatct tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc
    30061 ctcggtggcg cggatatgct gcgccgtgcg atgggtaaga aaaagccgga agagatggct
    30121 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    30181 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct
    30241 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag
    30301 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    30361 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    30421 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgccat caaaggggta
    30481 ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    30541 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    30601 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    30661 ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    30721 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    30781 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg cctgtacctg
    30841 accggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    30901 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    30961 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    31021 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    31081 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    31141 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    31201 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    31261 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    31321 gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    31381 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    31441 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    31501 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    31561 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    31621 gatctcggtg catggcagat tgcgcaactg gcacgccatc cgcagcgtcc ttataccctg
    31681 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg tgcgtatgca
    31741 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    31801 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    31861 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcct
    31921 atcatcacct ttatcgacac cccgggggct tatcctggcg tgggcgcaga agagcgtggt
    31981 cagtctgaag ccattgcacg caacctgcgt gaaatgtctc gccttggcgt accggtagtt
    32041 tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa
    32101 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    32161 ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    32221 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggcgctcac
    32281 cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat
    32341 ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    32401 ggttacgcgt aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg
    32461 ccacggtttg agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca
    32521 ttgccattat gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt
    32581 cggcgctggt ggcgcaaggc tttcagatta tctggccaca aaacagcgtt gatttgctga
    32641 aatttatcga acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc
    32701 tcgatttatg cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca
    32761 acacccactc gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg
    32821 aatatgcgct ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accaacgaat
    32881 atcttgataa cattacgccg ccgttcacga aagccttgtt tacctacgtc aaagagcgga
    32941 agtacacctt ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg
    33001 gctgtctgtt ttatgatttt ttcggcggga atacccttaa ggctgatgtc tctatttcgg
    33061 tcaccgagct tggttcgttg cttgaccaca ccgggccaca tctggaagcg gaagagtaca
    33121 tcgcgcggac ttttggcgcg gaacagagtt atatcgttac caacggaaca tcgacgtcga
    33181 acaaaattgt gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaact
    33241 gccataaatc actggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac
    33301 cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg ccgtgaattt actcgcgaca
    33361 gcatcgaaga gaaagtcgct gccactacgc aagcacaatg gccggttcat gcggtgatca
    33421 ccaactccac ctatgatggt ttgctctaca acaccgactg gatcaaacag acgctggatg
    33481 tcccgtcgat ccacttcgat tccgcctggg tgccgtatac tcattttcat ccaatctacc
    33541 agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa agttttcttc gaaacgcaat
    33601 cgacccacaa aatgctggcg gcgttatcgc aggcttcact gatccacatt aaaggcgagt
    33661 atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc
    33721 ccattgttgc ttcggttgag acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc
    33781 tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggctgcggg
    33841 aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat
    33901 gcaggcccgt tgcgcctggc gaacaatggc acggctttaa cgatgcggat gccgatcata
    33961 tgtttctcga tccggttaaa gtcactattt tgacaccggg gatggacgaa cagggcaata
    34021 tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt cctcgacgaa cgtgggatcg
    34081 tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggc atcgataaaa
    34141 ccaaagcaat gggattattg cgtgggttga cggaatttaa gcgctcttac gatctcaacc
    34201 tgcggatcaa aaatatgctg cccgatctct atgctgaaga tcccgatttc taccacaata
    34261 tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac gatctttccg
    34321 gtttgatgtt gcgggcattc gatactttgc cggaaatgat catgacgcca catcaggcat
    34381 ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag
    34441 tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa
    34501 tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt cccgtagggc
    34561 aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt
    34621 accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttcc gggcgagtaa
    34681 cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc
    34741 gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac
    34801 gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa
    34861 tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc
    34921 ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc
    34981 gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa
    35041 acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg
    35101 cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca
    35161 tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattctg gtggccttta
    35221 gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc
    35281 cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct
    35341 gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg
    35401 tacaacttgc gcaagagggg ctggggattg aggcccaggc gcggcaggca cgttatcagg
    35461 catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc
    35521 aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta
    35581 tggcggaagt ttcggagttt gctggaacac ggcttatccg cccgttgctt gcccgcacgc
    35641 ggggggaact ggagcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta
    35701 atcaggacga ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc
    35761 agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc cacactttgt gctgaacaag
    35821 agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga
    35881 cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg tcgcgcggcg attattcgcc
    35941 gctggctggc agggcagaat gcaccgatgc cttcccgcga cgcgctggtg cgcatctggc
    36001 aggaagtggc gctggcgcgg gaagatgctt ccccctgttt acgtttgggc gcctttgaaa
    36061 tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa agcgaaacca
    36121 ttgtgctgtg gcagacgtgg ctacaaccgc tgaaattacc ggcggggctg ggaaccgtac
    36181 agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt
    36241 tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg cggacgtaag ctgaagaaaa
    36301 tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac cacgccactg ctgttttatg
    36361 gagaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg
    36421 agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg
    36481 catcaaacgt cgcatccggc gaaagtcaat caggattcgc ttaccaccac cgtaccgatt
    36541 tcagggtggc taaagctggt aattttatcc agacgcagct cgcgggtttc gccagcggcc
    36601 tcgacgacca gatactccac atttttgcgg gagactaaat cactggcttt tgcctgcaat
    36661 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    36721 ttatcgtaat catcacaatt aattggttga tacgtatcat tcattgacat aatcgctcac
    36781 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    36841 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    36901 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    36961 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    37021 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    37081 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    37141 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    37201 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cttgctggcg
    37261 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    37321 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    37381 ccggatgagc gacgaattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc
    37441 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt
    37501 gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc
    37561 gatcgtacca tgacgctgca ggcaacgatt caggatggtg ttatctggtt atcggatgat
    37621 aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    37681 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgctg
    37741 gcgggcagca tgttaataag acctcaacgg ctattcatct gcgttttgac attcgggcgt
    37801 ccagcctgcc agagtattac aaagagcgtc tgctcgccgc cagccatcat ttgatcagca
    37861 gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    37921 ccgctctggc ccggctggtg gcagtgatta aagatttaac aaaagaacaa aaagcccgac
    37981 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    38041 caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatgg
    38101 atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga
    38161 tgtaataatc gggccgaagt cgatacgctt tctccggcgc aggctgccga actgaaaccg
    38221 atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt gcgaaggaat cgaaatctct
    38281 ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa
    38341 gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta
    38401 accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc
    38461 gatcgtgaag gcaatccaat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc
    38521 agtttaccta tgacgccgat gaccctgcga ggcatgtatt tttatatggc tgatgcggcg
    38581 accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag
    38641 cgtagctact tggctgcgcg cggtcacagt gaaaaaccgg tgttactgtc agtagaaggt
    38701 cactttacgc ttgaggctaa tccggatact ggtgcgccaa ccaaagtatt agcgcccgat
    38761 acggcgggta aattttaccc gaataaggat tgcagtagtt tggggcagta aacctgctta
    38821 aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt atcaggccta cagcatataa
    38881 tgcaacattt tgaattagca cgattttgta ggccggataa ggcgtttacg ccgcatccgg
    38941 cattttgtgc acgaggctgg ctttatcact gagtcagccc aacaaagcga cccgccttga
    39001 tataaatccc aggcttcagg tgcccaacat actgtaactg cgtttctggc ttaaacgcag
    39061 agacatcacc aatacgcata tgcatcagat cggcagggct aatccagccc gattgcgcca
    39121 gtgtaagtgg atgcccggat ttggcaaatg catcagtgac aaattccgaa caaaaccacg
    39181 actttttgtc tccttcgcac acactgctta actgcgcttt cgccaggtcg ctgacgcact
    39241 gttggcgaaa atcctcggag aacggattca gtgagcacat ctggcgagtc accataaagg
    39301 gaataaattc gacaatgccg cgatagttat aaccgctatc tttgatttta ttggcaaacg
    39361 cggtgatatc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg aaaagcttat
    39421 cactatgctt catcgctttt ttaagggaaa caatctggac gccagcgcct gtcgcttctg
    39481 caacgttatt atcacccaga aagattgcaa cgtgactcac agaggaagtg ctgaagacgc
    39541 ggattccaaa tgaggttacc ccaaggcttg aggagaacag caaatcgccg ggtttgagat
    39601 caggtgccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttga
    39661 cagcccatgt ttttgcctcg gcatctactg ctgtggctga tgggtctggc tggctgatat
    39721 caacggtaca agccgaaagt aaaagaaagc tggggagaag caggcggcag tacgcctttg
    39781 gtttatccat tttatacaat ccatgtaaaa aagggccctg aaattcagga ccctttctgg
    39841 catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt aattaactgt
    39901 ttctcgccgt tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc
    39961 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    40021 tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct gtacagtttc
    40081 tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat cgccacctgg
    40141 aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc
    40201 gcagctacca cacgcgttac cccgataccg tagcaaccca tcgtcaggat ttggttacgg
    40261 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    40321 atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctggccatc cgggcttgga
    40381 tcgccagcca ccacgttacg gatatctgca acttccgggg tagcgacatc gcgatcccag
    40441 ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    40501 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    40561 ccggctttaa ccacagcacg aatttcttct tcggtcgcga aagtcagcgg gcttgcaacc
    40621 tgcggcagtt tttctgcttt aacttcgttc agctcgtgat caccgcgcac cagcagcgca
    40681 accagcggga agctactgcc ttcaaccgct ttaaccagca gagtcttaac cgttttctca
    40741 atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc
    40801 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    40861 tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc accgctctgc
    40921 gccagcacct ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc ggcctgtacg
    40981 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    41041 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    41101 tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    41161 tgatagaagt tcagcggcag ctgtttgtaa gagctaagct cgttacgaat caggtcagtg
    41221 ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc
    41281 agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga
    41341 accaccggca tcgacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    41401 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    41461 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    41521 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    41581 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc
    41641 aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa
    41701 aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg tagccagacg gcataggttt
    41761 tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga
    41821 taaacagcgt taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg
    41881 cggtaaaact caccgccatc tctgcagctg gcgcactttg cgcatagctg gcactggcat
    41941 cgggaagcga ttcggcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga
    42001 ccagatccag actgccgagc ttcagaatca cgctgccttt atggcaaaca acctctttca
    42061 gctctaccag cgacatgcca attgggttag ggcggaaagt agagcgtgtt gcgaaaaccc
    42121 ccattctggc gttaccgccg aggcgcggtg gacgcacagt cggacgccag ccgccttcca
    42181 tcgtttgatg aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg
    42241 cgtcggcctg gttgtaggaa gcaatgagat gcagttcgcc gttggcgctt tttaccagac
    42301 ctggctggcg cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct
    42361 cgaactggaa actgctcatt tcgccgtaat gttaagcgca gagccgatac atacagcctg
    42421 acgatagcag cccggcgtac cgctggtgac ttcgcagcta tgcagtaata cagcattggc
    42481 tttcatttta gaggcattga tttgcatctg cttacgtgcg gttggaatgc tcggcggaga
    42541 gtcctgatta gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt
    42601 gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg
    42661 ttttgcaggc tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct
    42721 gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat
    42781 agtatttcct caatgtatga tcaaaacgtc aatattgaat caggagcttg taaaaatgac
    42841 aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaaaatta
    42901 ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcatcaga
    42961 ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac
    43021 gatcaggttt acgtatgggg agtctttatc ttcgacaaag ataccgtctt tcgccggagt
    43081 caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc
    43141 cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc
    43201 aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac
    43261 tttttgcagc agcagcagtg aacgaccaag attagttgga tcgtttggca cggcaacctg
    43321 cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata
    43381 aacaaaagtg ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag
    43441 gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg
    43501 cagaacatag tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac
    43561 tttctgcgcg acttctgcta cctgctgttc ggcaccaaca atcacgccga ctttaatgtg
    43621 gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc
    43681 cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca attatttatg
    43741 ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat
    43801 taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc
    43861 gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat
    43921 cgcggaataa ccgaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa
    43981 cgcttccggt aacagcactt tacagacgat ctgcattggc gtggcaccca ttgcgcggga
    44041 agcttcaatt aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa
    44101 cggtgctgca ccaacggtta acggaacaat tgctgcctgc aaaccaatcg atgtaccgac
    44161 aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa
    44221 aatgttcaca atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg
    44281 acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc acaaacccaa aaaatccgga
    44341 tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc
    44401 agacataacc cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt
    44461 gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat gccgaattta acgccaccgg
    44521 cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag
    44581 aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt
    44641 cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg
    44701 tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg
    44761 tgtcctgctc gatcagttcg ccattgctga tgaccgccac gcaatcacaa atacgcttca
    44821 caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt
    44881 tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac
    44941 acagcaatac tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc
    45001 caccggaaag attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca
    45061 attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag
    45121 ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa
    45181 tctggcggcg agctttggtc aactcggatt ctgacagcgt ggtcaattcc tggccatcga
    45241 ccagcacgct accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct
    45301 tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga
    45361 cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa
    45421 gttttatcat tgattattta ttatcgtcat taagttagtc gtagcatctc gaatgcctga
    45481 aacgggcaac gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg
    45541 tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga tactagcgtc ccatgcctta
    45601 ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca
    45661 ccattaatgt cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg
    45721 ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc
    45781 agtctggcat tgctcgcggt aaatttaccg aagcacagtt tgaaacgctg accgagtgga
    45841 tggactggtc gctggcggac cgagatgtcg atctggatgg tatctattat tgcccgcatc
    45901 atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga ttgccgcaaa ccacatccgg
    45961 ggatgctttt gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg
    46021 gcgataaatt agaagatatg caggcagcgg ttgcggcgaa cgtgggaaca aaagtgctgg
    46081 tgcgtacggg taaacctatt acacctgaag cagaaaacgc ggcagattgg gtgttaaata
    46141 gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgcaa tgattaaaag
    46201 atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagccga ctccctataa
    46261 tgcgcctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    46321 gagaaaaaat cctgaaattc agggttgact ctgaaagagg aaagtgtgat atacgccacc
    46381 tcgcgacagt gagctgaaag ctgcgtcgc
//
LOCUS       DADLDT020000017        45785 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000017 DADLDT020000000
VERSION     DADLDT020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.017"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            95..2248
                     /locus_tag="JQH22_000907"
     CDS             95..2248
                     /locus_tag="JQH22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFB2205318.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="JQH22_000908"
     CDS             2301..4031
                     /locus_tag="JQH22_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205319.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQH22_000909"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQH22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFB2205320.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="JQH22_000910"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="JQH22_000910"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFB2205321.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="JQH22_000911"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="JQH22_000911"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFB2205322.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQH22_000912"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQH22_000912"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFB2205323.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="JQH22_000913"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="JQH22_000913"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFB2205324.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="JQH22_000914"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="JQH22_000914"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFB2205325.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="JQH22_000915"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="JQH22_000915"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFB2205326.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="JQH22_000916"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="JQH22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFB2205327.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQH22_000917"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQH22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFB2205328.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQH22_000918"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQH22_000918"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFB2205329.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQH22_000919"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQH22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFB2205330.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQH22_000920"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQH22_000920"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFB2205331.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQH22_000921"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQH22_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFB2205332.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="JQH22_000922"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="JQH22_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFB2205333.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="JQH22_000923"
     CDS             16289..16828
                     /locus_tag="JQH22_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFB2205334.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="JQH22_000924"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="JQH22_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFB2205335.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="JQH22_000925"
     CDS             complement(18187..18534)
                     /locus_tag="JQH22_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HFB2205336.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="JQH22_000926"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="JQH22_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFB2205337.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="JQH22_000927"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="JQH22_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFB2205338.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="JQH22_000928"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="JQH22_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFB2205339.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="JQH22_000929"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="JQH22_000929"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFB2205340.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="JQH22_000930"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="JQH22_000930"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFB2205341.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="JQH22_000931"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="JQH22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="JQH22_000932"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="JQH22_000932"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFB2205342.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="JQH22_000933"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="JQH22_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFB2205343.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="JQH22_000934"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="JQH22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFB2205344.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="JQH22_000935"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="JQH22_000935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFB2205345.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="JQH22_000936"
                     /pseudo
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="JQH22_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="JQH22_000937"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="JQH22_000937"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFB2205346.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="JQH22_000938"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="JQH22_000938"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFB2205347.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="JQH22_000939"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="JQH22_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFB2205348.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="JQH22_000940"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="JQH22_000940"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFB2205349.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="JQH22_000941"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="JQH22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFB2205350.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="JQH22_000942"
     CDS             complement(39992..40447)
                     /locus_tag="JQH22_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205351.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="JQH22_000943"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="JQH22_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="JQH22_000944"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="JQH22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFB2205352.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="JQH22_000945"
     CDS             complement(41290..41967)
                     /locus_tag="JQH22_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFB2205353.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42228..42416)
                     /gene="yaiA"
                     /locus_tag="JQH22_000946"
     CDS             complement(42228..42416)
                     /gene="yaiA"
                     /locus_tag="JQH22_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFB2205354.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="JQH22_000947"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="JQH22_000947"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFB2205355.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="JQH22_000948"
     CDS             complement(43173..43631)
                     /locus_tag="JQH22_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFB2205356.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="JQH22_000949"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="JQH22_000949"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFB2205357.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="JQH22_000950"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="JQH22_000950"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFB2205358.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11905 a        11939 c        11199 g        10742 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatctacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttatga tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaattgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgc
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgtgcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt tcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaattttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccaa
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaaca ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccaa gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt cgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ctagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcagatggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatcgtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtggc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga tagtcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caagcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttat tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcacta tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgagatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DADLDT020000018        44109 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000018 DADLDT020000000
VERSION     DADLDT020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44109
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.018"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            46..120
                     /locus_tag="JQH22_000951"
     tRNA            46..120
                     /locus_tag="JQH22_000951"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Gln,seq:ctg)
     gene            158..232
                     /locus_tag="JQH22_000952"
     tRNA            158..232
                     /locus_tag="JQH22_000952"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:190..192,aa:Gln,seq:ctg)
     gene            complement(386..1561)
                     /gene="ubiF"
                     /locus_tag="JQH22_000953"
     CDS             complement(386..1561)
                     /gene="ubiF"
                     /locus_tag="JQH22_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HFB2205359.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1707..3131
                     /gene="miaB"
                     /locus_tag="JQH22_000954"
     CDS             1707..3131
                     /gene="miaB"
                     /locus_tag="JQH22_000954"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFB2205360.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3284..4324
                     /locus_tag="JQH22_000955"
     CDS             3284..4324
                     /locus_tag="JQH22_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFB2205361.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4321..4788
                     /gene="ybeY"
                     /locus_tag="JQH22_000956"
     CDS             4321..4788
                     /gene="ybeY"
                     /locus_tag="JQH22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFB2205362.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4878..5756
                     /gene="corC"
                     /locus_tag="JQH22_000957"
     CDS             4878..5756
                     /gene="corC"
                     /locus_tag="JQH22_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFB2205363.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5781..7319
                     /gene="lnt"
                     /locus_tag="JQH22_000958"
     CDS             5781..7319
                     /gene="lnt"
                     /locus_tag="JQH22_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFB2205364.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7716..8624
                     /gene="gltI"
                     /locus_tag="JQH22_000959"
     CDS             7716..8624
                     /gene="gltI"
                     /locus_tag="JQH22_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFB2205365.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8794..9534
                     /gene="gltJ"
                     /locus_tag="JQH22_000960"
     CDS             8794..9534
                     /gene="gltJ"
                     /locus_tag="JQH22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFB2205366.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9534..10208
                     /gene="gltK"
                     /locus_tag="JQH22_000961"
     CDS             9534..10208
                     /gene="gltK"
                     /locus_tag="JQH22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFB2205367.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10208..10933
                     /gene="gltL"
                     /locus_tag="JQH22_000962"
     CDS             10208..10933
                     /gene="gltL"
                     /locus_tag="JQH22_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HFB2205368.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11051..11986
                     /gene="rihA"
                     /locus_tag="JQH22_000963"
     CDS             11051..11986
                     /gene="rihA"
                     /locus_tag="JQH22_000963"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFB2205369.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12070..13731
                     /gene="hscC"
                     /locus_tag="JQH22_000964"
                     /pseudo
     CDS             12070..13731
                     /gene="hscC"
                     /locus_tag="JQH22_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13791..15242)
                     /gene="djlC"
                     /locus_tag="JQH22_000965"
     CDS             complement(13791..15242)
                     /gene="djlC"
                     /locus_tag="JQH22_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFB2205370.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15239..15946)
                     /locus_tag="JQH22_000966"
     CDS             complement(15239..15946)
                     /locus_tag="JQH22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFB2205371.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            16048..16602
                     /locus_tag="JQH22_000967"
     CDS             16048..16602
                     /locus_tag="JQH22_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFB2205372.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16612..18039)
                     /locus_tag="JQH22_000968"
     CDS             complement(16612..18039)
                     /locus_tag="JQH22_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFB2205373.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18036..18743)
                     /locus_tag="JQH22_000969"
     CDS             complement(18036..18743)
                     /locus_tag="JQH22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFB2205374.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18907..19884
                     /locus_tag="JQH22_000970"
     CDS             18907..19884
                     /locus_tag="JQH22_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFB2205375.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19954..20436)
                     /locus_tag="JQH22_000971"
     CDS             complement(19954..20436)
                     /locus_tag="JQH22_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFB2205376.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20671..23253
                     /gene="leuS"
                     /locus_tag="JQH22_000972"
     CDS             20671..23253
                     /gene="leuS"
                     /locus_tag="JQH22_000972"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFB2205377.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23268..23849
                     /gene="lptE"
                     /locus_tag="JQH22_000973"
     CDS             23268..23849
                     /gene="lptE"
                     /locus_tag="JQH22_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFB2205378.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23849..24880
                     /gene="holA"
                     /locus_tag="JQH22_000974"
     CDS             23849..24880
                     /gene="holA"
                     /locus_tag="JQH22_000974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFB2205379.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24882..25523
                     /gene="nadD"
                     /locus_tag="JQH22_000975"
     CDS             24882..25523
                     /gene="nadD"
                     /locus_tag="JQH22_000975"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFB2205380.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25547..26149
                     /locus_tag="JQH22_000976"
     CDS             25547..26149
                     /locus_tag="JQH22_000976"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFB2205381.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDIFATLRVLNSRAIGVENA"
     gene            26409..26726
                     /gene="rsfS"
                     /locus_tag="JQH22_000977"
                     /gene_synonym="rsfA"
     CDS             26409..26726
                     /gene="rsfS"
                     /locus_tag="JQH22_000977"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFB2205382.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26730..27197
                     /gene="rlmH"
                     /locus_tag="JQH22_000978"
     CDS             26730..27197
                     /gene="rlmH"
                     /locus_tag="JQH22_000978"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFB2205383.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27228..29129
                     /gene="mrdA"
                     /locus_tag="JQH22_000979"
     CDS             27228..29129
                     /gene="mrdA"
                     /locus_tag="JQH22_000979"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFB2205384.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29132..30244
                     /gene="mrdB"
                     /locus_tag="JQH22_000980"
                     /gene_synonym="rodA"
     CDS             29132..30244
                     /gene="mrdB"
                     /locus_tag="JQH22_000980"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFB2205385.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30255..31343
                     /gene="rlpA"
                     /locus_tag="JQH22_000981"
     CDS             30255..31343
                     /gene="rlpA"
                     /locus_tag="JQH22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFB2205386.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31482..32693
                     /gene="dacA"
                     /locus_tag="JQH22_000982"
     CDS             31482..32693
                     /gene="dacA"
                     /locus_tag="JQH22_000982"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFB2205387.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32804..33067
                     /locus_tag="JQH22_000983"
     CDS             32804..33067
                     /locus_tag="JQH22_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFB2205388.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33168..33809
                     /gene="lipB"
                     /locus_tag="JQH22_000984"
     CDS             33168..33809
                     /gene="lipB"
                     /locus_tag="JQH22_000984"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFB2205389.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34068..35021
                     /locus_tag="JQH22_000985"
     CDS             34068..35021
                     /locus_tag="JQH22_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFB2205390.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35230..36195
                     /gene="lipA"
                     /locus_tag="JQH22_000986"
     CDS             35230..36195
                     /gene="lipA"
                     /locus_tag="JQH22_000986"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFB2205391.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36296..36499)
                     /gene="tatE"
                     /locus_tag="JQH22_000987"
     CDS             complement(36296..36499)
                     /gene="tatE"
                     /locus_tag="JQH22_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFB2205392.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36628..37416)
                     /locus_tag="JQH22_000988"
     CDS             complement(36628..37416)
                     /locus_tag="JQH22_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFB2205393.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37509..37892
                     /gene="crcB"
                     /locus_tag="JQH22_000989"
     CDS             37509..37892
                     /gene="crcB"
                     /locus_tag="JQH22_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFB2205394.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37945..38154)
                     /gene="cspE"
                     /locus_tag="JQH22_000990"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37945..38154)
                     /gene="cspE"
                     /locus_tag="JQH22_000990"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFB2205395.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38329..38889)
                     /gene="pagP"
                     /locus_tag="JQH22_000991"
     CDS             complement(38329..38889)
                     /gene="pagP"
                     /locus_tag="JQH22_000991"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFB2205396.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39477..40862
                     /gene="dcuC"
                     /locus_tag="JQH22_000992"
     CDS             39477..40862
                     /gene="dcuC"
                     /locus_tag="JQH22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFB2205397.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(40903..41244)
                     /locus_tag="JQH22_000993"
                     /pseudo
     CDS             complement(40903..41244)
                     /locus_tag="JQH22_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41582..42091)
                     /locus_tag="JQH22_000994"
     CDS             complement(41582..42091)
                     /locus_tag="JQH22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFB2205398.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42254..42499
                     /locus_tag="JQH22_000995"
     CDS             42254..42499
                     /locus_tag="JQH22_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2205399.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42474..44105
                     /locus_tag="JQH22_000996"
                     /pseudo
     CDS             42474..44105
                     /locus_tag="JQH22_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        11099 a        11030 c        11004 g        10976 t
ORIGIN      
        1 tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag
       61 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
      121 atttattcaa gacgcttaca tcgtaagtgc acccagttgg ggtatcgcca agcggtaagg
      181 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa
      241 aagctcgctt cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg
      301 acacgtctta tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggctg
      361 gcacgcatcc ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt
      421 tcaacacgcc agcacgctct gccgccatta acccgagatt acgcacaaaa cgcagcggcg
      481 gcagattatt actgaatccg gcataaaaca gatccatacc actttgcata atgaagttat
      541 ccgccatgcg ccgcatctgg taacgcttga ggacaggata actggcccac gcttcaccgt
      601 agctgcgggc attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattca
      661 ccccctgccc cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg
      721 gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg
      781 taacgtaacc cagacgcgac gggaaatgct tcgcgatttc cgcctggagt tgcgccatat
      841 tcatattctg caactggcga atacgcgccg gagagtcata ccacaccagc gatgcccagt
      901 tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt
      961 cgccgggatc gttctcacac tgtacgctaa tcaacatgca cgactgcgca tactgccagg
     1021 catgaacgcc aattcccgcc atctgccgca cctgagaatt tgcgccgtcg gcaccaatca
     1081 ccagtttcgc gcggatcgtt tcaccgccct tcagttccag ctcctgaaga tcattatctc
     1141 gatgtaatgc aatcagcgag gttggcacac gtaacgttac tttcggatgc gcttccagcg
     1201 cctgccacaa cgcctgttgc aggactgtgt tttccaccat gtagccaagc aacggcaact
     1261 ttaattcggc tgcgtcaaat accacatgtg ccgtttccca ctcccaggtt tccagtctgc
     1321 ggtaaggatg gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg
     1381 ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg ttggctgtca gcgacaaacg
     1441 gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg
     1501 ccagtgcgcc gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca
     1561 tggtcaatca tcctgttagc aatatgcttt aagtttacag gatttttacc cccaagggct
     1621 gataacgctc atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct
     1681 ggctactatt tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct
     1741 gtcagatgaa cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct
     1801 atcaactgac cgacgtggcg gaagaagcgg atgtgctact gctgaacacc tgctcaatcc
     1861 gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga
     1921 agaatccaga cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca
     1981 ttcgccagcg cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc
     2041 cggagatgat caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg
     2101 aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga agggccgacc gcgtttgtct
     2161 ccatcatgga aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg
     2221 aagaggtaag ccgtccgtct gacgatattc tgtttgagat tgcccagctt gcggctcagg
     2281 gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg
     2341 acggcaccac cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg
     2401 atcgtattcg ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt
     2461 accgcgacac gcctgagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc
     2521 gcattctgaa cctgatggga cgtacccata cggcgctgga gtacaaagcg atcatccgta
     2581 aactgcgtgc ggcgcgtccg gatattcaga tcagttctga cttcattgtt ggcttccctg
     2641 gcgaaaccac cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca
     2701 tgagctacag ctttatcttc tctgcacgtc caggaacgcc tgccgccgat atggttgatg
     2761 atgttccgga agaagagaag aagcagcgtc tgtatattct gcaagaacgt atcaatcagc
     2821 aagcgatggc atggagccgt cggatgctcg gcaccaccca gcgtattctg gtagaaggga
     2881 cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga aaataaccgc gtggtcaact
     2941 tcgagggcac accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc
     3001 cgaactctct gcgcggtaaa gtggtgcgaa ctgaagatga aatgggtctg cgcatggcag
     3061 aaacaccgga gtcggtgatt gcccgcaccc gcaaagaaaa cgaccttggc gtgggttatt
     3121 atcagccgta attctcaggc ctgccgttcc ggtgggcctt gttttttctc tctttatccc
     3181 catctttatc gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg
     3241 gtgaatgttc agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg
     3301 cgaaatcacc ctggagccag cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga
     3361 tgacaacatc aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgataacca
     3421 ctttaaactg accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct
     3481 gtatgtcgat actgccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca
     3541 ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg gagagcgtgc cggagtacgg
     3601 caaagcggtc aatatcaaaa ctaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc
     3661 gcagtacatc gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac
     3721 gggtaaaacc tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggaaattcg
     3781 ccgtattctg ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg
     3841 cgatttaagc cagaaagtag acccgtatct gcgcccactg tacgacgcgc tgtttgaaat
     3901 gctgggcttt gagaaagtcg agaaactgat tgagcgcaac gttattgaag tcgcgccgct
     3961 ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc attctcgatg agagccagaa
     4021 cactaccatc gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt
     4081 tatcaccggc gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca
     4141 tgccattgaa gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga
     4201 cgtggttcgt cacccggtgg tggcgcgtat cgttaacgct tatgaagcct gggaagaagc
     4261 agaacaaaaa cgtaaagcgg cgctggcagc agaacgcaag cgcgaagaac aggaacaaaa
     4321 atgagtcagg tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa
     4381 gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agagtcggaa
     4441 gtgactattc gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt
     4501 aaggataagc cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggagatg
     4561 tcgctactgg gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa
     4621 ggcaaaccac tggaggcgca ttgggcgcat atggtggtgc acggcagtct gcatttgtta
     4681 ggttacgatc acattgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt
     4741 atgcttgctc tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat
     4801 ttgactggca gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg
     4861 agaacccata cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca
     4921 agaagggatt tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg
     4981 acgaactgct ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc
     5041 gcgatatgct cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc
     5101 cccgctccca gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca
     5161 tcatcgagtc cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag
     5221 ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca
     5281 tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc
     5341 tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg
     5401 tttccggtct ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg
     5461 agtatgacga agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc
     5521 gcgcactggc ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag
     5581 aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg
     5641 aaactatcga catcgacggt taccagttca aagtggcgat ggccgatagt cggcgtatta
     5701 ttcaggttca tgtcaaaatc ccggatgact caccccagcc aaagctggat gaataaaacc
     5761 gaaactggat agataactac atggcttttg cctcattaat tgaacgccag cgtattcgcc
     5821 tgctgctggc gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgacgtct
     5881 ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgtcgcccac
     5941 tccagtctgc cgctattggc ttttgctggg gatttggcct ctttggcagc ggtattaact
     6001 gggtctatgt cagcatcgcg acctttggcg ggatgcctgg cccggttaac atcttcctgg
     6061 tggtgctgct ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc
     6121 gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc cacccctgct ctctggcaag
     6181 tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc gtggttacag ttcggctata
     6241 gccagattga tggcccgtta aaagggctgg caccgctaat gggcgtggaa gccattaact
     6301 tcctgctgat gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc
     6361 cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac atccaatggt
     6421 ttaccccgca accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat
     6481 cgctgaaatg ggacgaaggc cagcttctta atacgctaaa gatttactac aacgcaacgg
     6541 caccgctgat gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg
     6601 aaattaatca gcaaccgttc ctcaaagcac tggacggtga gttgcgtgat aaaggtagct
     6661 cgctggtaac cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca
     6721 acaccatcat cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc gatcgctata
     6781 acaaaaacca tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt
     6841 tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc
     6901 cgctgtcggc aaatggtatt gagcttactg cggctatttg ctacgagatc attctcgccg
     6961 agcaagtgcg cgataacttc cgcccggata ccgactacct gctgactatc tccaacgatg
     7021 cgtggtttgg taaatctatt ggtccatggc aacacttcca gatggcgcga atgcgtgcgc
     7081 tggagctggc gcgcccactg ttgcgcagca ccaacaacgg cattacggcg gtgattggcc
     7141 cgcagggtga gattcaggcg atgatcccgc agttcgcccg cgaggtgtta accactaacg
     7201 tgacgccgac caccggactc acaccatacg cacgtaccgg caactggccg ctgtgggtgc
     7261 tgacggcact gtttggtttt gctgctgtgt tgatgagtct gcgtcagcga cgtaaataat
     7321 cccttcttat cgtgccggaa tacatcccat atttcggcac gctcaccgga tgcggcatca
     7381 gtgcattatc cggataacaa tattctccta tccaactttg gcacatctat tgctttgtta
     7441 tacaaggcaa agcttgaacc agcatcagta caacccggtt gcaccacagc aatccaccgg
     7501 tagcaccaaa agagtgcatt ttaagaattt tgcttcagaa tggtgcggtg cacctcgcaa
     7561 aaacaaacaa taaaacatca atatctttac atttagccaa actaaatgtt acctaacagg
     7621 cacaacactg cacaataaag ttgcagacga taacaacaca aacactcaca acgggtatcc
     7681 atgcgttctt aacgcagaag ataaaggtgt tggatatgca attacgtaaa cctgccacag
     7741 caatcctcgc cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag
     7801 caggcagtac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat
     7861 cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt
     7921 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa
     7981 aactgattcc gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt
     8041 ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca
     8101 ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg cggcgatatc aaagattttg
     8161 ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca
     8221 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
     8281 actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgctctgc
     8341 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc
     8401 agtctcagga agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga
     8461 tggatgacac tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat
     8521 ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa
     8581 tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg
     8641 cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg
     8701 ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta
     8761 acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt
     8821 tttttacaac aagccccgtt cggcaatacc acctatcttg gttgggtctg gagtggtttt
     8881 caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt
     8941 ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc
     9001 gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg
     9061 gagctgctgc cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag
     9121 tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag
     9181 gtacgcgccg ctattcagtc gctaccgcgt gggcagaaga atgccgcgct ggcgatgggc
     9241 ctgacgctac cgcagactta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc
     9301 ccgccgatga cttccgagat gatgaacctg gtgaaaaact ccgccatcgc ctcgaccatc
     9361 ggtctggtgg atatggcggc gcaggcgggt aaattgctcg actactcggc tcacgcctgg
     9421 gagtcgttta ccgccatcac gcttgcgtat gtgttgatta acgcttttat catgctggtg
     9481 atgacgctgg ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa ataatgtacg
     9541 agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca
     9601 ccctgaaaat caccgtcacg gcagtcgtga ttggtatttt gtggggtacc atgctggcgg
     9661 tgatgcgctt gtccagcttc gcgcctgttt cgtggtttgc caaagcctac gttaacgtat
     9721 tccgctcgat tcctttagtc atggttttgc tgtggtttta cttgattgtg ccgggatttc
     9781 tgcaaaatgt gctgggatta tcgccaaaaa atgatattcg tttaatttcg gcgatggtgg
     9841 cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgccggt attcaaagta
     9901 tttcccgtgg tcagtcgagc gccgcgctgg cgttggggat gactcactgg cagtcgatga
     9961 aactgattat tctgccgcag gctttccgcg cgatggtgcc gctgctgctc actcagggca
    10021 tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta
    10081 ccgcctcaac cattggtgag cgtgatggta cgcaggtcga gatgatcctg tttgccggat
    10141 ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga
    10201 cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac
    10261 cgactgctca accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc
    10321 cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat
    10381 caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc tgcgctcccg
    10441 cgtcgggatg gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct
    10501 gaccctggcg caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt
    10561 gaaattgctg gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc
    10621 cggcggtcag cagcagcgcg tggcaatcgc tcgcgcattg tgtatggctc ctattgcgat
    10681 gctgttcgac gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt
    10741 gatggtggaa ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt
    10801 tgcccgcaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc
    10861 gccgaaagac gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa
    10921 aatcctgcat taatcactct ggcgcacata cccaattgcg cgccatctca cgctttatcg
    10981 tttcgcccct cgtatctgtt acatcctggc gttacccttg tcgcaaagaa gcacacaaca
    11041 aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg atgacgctat
    11101 cgcaatagtt ctcgcactcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc
    11161 cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa
    11221 tcgcaccgat attccggtgg caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat
    11281 cgcggacaat gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccgacgtt
    11341 cgcaccgcaa aactgtacgg cggtggagct gatggcgaaa acgctgcgcg aaagtgcgga
    11401 acctgtcacg attgtttcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca
    11461 tccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg
    11521 taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt
    11581 cttccagtca gggatcccgg tggtgatggc cggtctggat gttactcata aagcgcaaat
    11641 ccacgttgaa gacaccgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc
    11701 cgaactgctg gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc
    11761 accgctgcat gacccatgca ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt
    11821 tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc cagggtatga cggttgttga
    11881 ttattattat ctgactggca ataaaccgaa tgccactgta attgtcgatg ttgatcgtca
    11941 gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaacact cgtcattgat
    12001 taagatcacc acgcgttacc tgggtggtct taatctattt atctcttttt ctttcaagat
    12061 ataaatgcaa tggataatgc agaactcgcc ataggtatcg atctcggcac taccaatagt
    12121 ttaattgccg tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat
    12181 ttaacaccat ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct
    12241 gtatcacggc gtacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc
    12301 agctggcggt taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc
    12361 tcattaaaag aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc
    12421 gttccggctt atttcagtga tgaacaacgc aagcataccc gtttagcagc ggacttagcc
    12481 gggttaaatg cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg
    12541 catacccaac aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt
    12601 acggtgcttg agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc
    12661 ctcggtggcg aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc
    12721 acaaggacca cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca
    12781 aaatgtagca atcaatcgcc aatgcacatt cgctggtagt accaggatga aacgcgggaa
    12841 tgcgaatttt acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga
    12901 gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg
    12961 gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt
    13021 ggcaaattac cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc
    13081 caggccgcct gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc
    13141 ccttactcgt tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt
    13201 attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg
    13261 gaacaggatt caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac
    13321 attctggtgg aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat
    13381 attcgcttta gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc
    13441 agcgttaagt ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa
    13501 gagagtcgga cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc
    13561 acctttaaag ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag
    13621 attggccggg tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc
    13681 gatgaggtca ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg
    13741 ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac
    13801 aacatcgcga ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata
    13861 atgctggtaa tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt
    13921 gttggctcat cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg
    13981 gtgataatgg cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc
    14041 agcatctctt gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc
    14101 agtaacggtg aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct
    14161 ttgcgtttga agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta
    14221 tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc
    14281 ggttgacctt gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa
    14341 taatccgggc aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg
    14401 taactttccc agtacgcgcc gagatcagca agcaggcttt ccccctcgcc gcagtacaaa
    14461 cgttgtgcac actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt
    14521 ttggctacag gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt
    14581 ttgcgatgta actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca
    14641 gtgacatagt caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc
    14701 tggttctgta acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc
    14761 tgaatggcct caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac
    14821 gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct
    14881 tctatagcga ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta
    14941 tttaattgct gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc
    15001 agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca
    15061 tctgcttcct cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat
    15121 gctttaaacc cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc
    15181 tggcggataa tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc
    15241 ataggtcgct cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc
    15301 agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc
    15361 cggcgctatc gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact
    15421 gagaagattg ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt
    15481 gatagtaatg gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg
    15541 tcagccagtt gttgagcaca ccggcccgtg acagaaaccc cattcgaaca taatcccagg
    15601 ctttgattcc cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca
    15661 accctctctc cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc
    15721 atgtcagata taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag
    15781 tctgaattaa atcatcacga ttatttattc cccaggatga tgagagatcg aattctttta
    15841 tcccggtata catttttggc gagtcatgcg cgggtttgca actctgatta agtatttcca
    15901 tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt attcattttt taataatccc
    15961 ttaagtgcat taactgacta cttttttatt attgatgagt ataaacttga cgctttcaaa
    16021 ataaaaagaa aatcgaagca ttcacacatg aataaaaaat taatgtatat attcgcaatt
    16081 tttatagttg cagcaattac ctgtattagc caacccaaga aaacgatgtt gcgtgataaa
    16141 gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg
    16201 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
    16261 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
    16321 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactgaggtg gatctacctg
    16381 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
    16441 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
    16501 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
    16561 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
    16621 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
    16681 gacgcccaga cagcccagcc cgccatcatc aacgggcatt ggctgtggtt gcggctccgg
    16741 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
    16801 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
    16861 ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
    16921 atgttctgta tcacgcgtga gtgagatccg cggcttacgt ccttcgtgca aaaccatctc
    16981 gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
    17041 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag
    17101 atcacgcgct tcaatcatca tcaccactaa tgggtaataa tagacccgat gctgacggca
    17161 ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag
    17221 ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg
    17281 cgcatcttca ttgtcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg
    17341 gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
    17401 ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga
    17461 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caactggcag
    17521 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
    17581 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
    17641 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
    17701 ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg
    17761 ccaggcggag gacagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
    17821 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
    17881 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
    17941 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
    18001 cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc
    18061 gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca
    18121 tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
    18181 gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga
    18241 ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga
    18301 tgaatacgcg actggatcca tagactttct tcttcactca accagttatt aagtacgccc
    18361 attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag
    18421 cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca
    18481 agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
    18541 cgagcggcat gaccgttctc gatcattcgg tgaattatgc gcagcaggtc atcacggtca
    18601 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat
    18661 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
    18721 attttttgcg accccatgtc catataattt atccttaaac attcatttaa tgaatgtaaa
    18781 ctacatttcg aggctcagtt atttatactt ttctgacgca acataatacg cataaaataa
    18841 ggcttacgca aaaatggaat acataaaaat ataatatatt ttatctacaa ccataaggaa
    18901 atgataatga tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
    18961 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
    19021 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag
    19081 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
    19141 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
    19201 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa
    19261 gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
    19321 ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg
    19381 tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat
    19441 aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
    19501 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
    19561 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
    19621 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
    19681 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
    19741 tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
    19801 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
    19861 cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc
    19921 atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
    19981 ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
    20041 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
    20101 cccatgatga ttgaggtcct ggaaaatttc gcgccattca agctgcgttt tatcggtgat
    20161 gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc
    20221 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg
    20281 cgtcagctca tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
    20341 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc
    20401 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
    20461 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
    20521 aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
    20581 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
    20641 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
    20701 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
    20761 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
    20821 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
    20881 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
    20941 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
    21001 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
    21061 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
    21121 aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
    21181 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
    21241 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
    21301 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
    21361 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
    21421 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
    21481 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
    21541 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt
    21601 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
    21661 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
    21721 aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt
    21781 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
    21841 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
    21901 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gccagcgtta ctggggcgcg
    21961 ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg
    22021 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
    22081 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
    22141 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
    22201 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
    22261 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
    22321 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
    22381 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
    22441 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
    22501 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
    22561 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
    22621 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
    22681 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
    22741 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
    22801 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
    22861 gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg
    22921 caggaagcac tgcaggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc
    22981 acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
    23041 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
    23101 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
    23161 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
    23221 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
    23281 caacactgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
    23341 acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
    23401 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
    23461 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
    23521 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
    23581 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt
    23641 cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
    23701 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
    23761 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
    23821 cacgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
    23881 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
    23941 gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca
    24001 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
    24061 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
    24121 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctattgat
    24181 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
    24241 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
    24301 tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
    24361 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
    24421 ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
    24481 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
    24541 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
    24601 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
    24661 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
    24721 tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
    24781 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
    24841 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
    24901 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
    24961 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
    25021 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
    25081 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc
    25141 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
    25201 gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
    25261 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
    25321 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
    25381 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
    25441 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta
    25501 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
    25561 aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac
    25621 acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt
    25681 ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
    25741 tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg
    25801 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
    25861 gtggtgcaat gactggcagc atgctatccc cacgaatggt gaaggatttc aggcattttc
    25921 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt
    25981 aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc
    26041 cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatattcgc
    26101 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aacgcatgac ttttctgttt
    26161 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
    26221 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    26281 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    26341 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    26401 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    26461 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    26521 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    26581 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    26641 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    26701 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    26761 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    26821 tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg gacatcaagc
    26881 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    26941 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    27001 ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    27061 cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    27121 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    27181 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    27241 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    27301 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    27361 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    27421 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta
    27481 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    27541 tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    27601 gttcacaccg tttcacctct attccggtga aaactaacct aaccgaagta caagtagctc
    27661 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    27721 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    27781 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    27841 ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    27901 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    27961 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    28021 tgctggcggg tagccgcgca gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg
    28081 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    28141 attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    28201 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg
    28261 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    28321 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    28381 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    28441 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    28501 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    28561 agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgact ccaatccaga
    28621 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    28681 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    28741 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    28801 gccctaatgg tacggcgaat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    28861 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    28921 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    28981 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    29041 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    29101 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    29161 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    29221 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    29281 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    29341 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    29401 tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    29461 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    29521 cgacgtttgc ccgccatcgt tgaagaatac tggcatcgcg ctggtgctga tatttatgcc
    29581 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    29641 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    29701 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    29761 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    29821 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    29881 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    29941 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    30001 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    30061 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    30121 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    30181 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    30241 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    30301 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    30361 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    30421 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    30481 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    30541 cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg cttccgatcc
    30601 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    30661 atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc
    30721 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    30781 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    30841 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    30901 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    30961 gtagcggttt cctcggcgct ccaacgacct tagcgcctag tgtactggaa ggcagcgaac
    31021 cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca acctcgcctg
    31081 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    31141 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    31201 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    31261 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    31321 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    31381 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    31441 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    31501 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    31561 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    31621 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    31681 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    31741 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    31801 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    31861 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    31921 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    31981 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    32041 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    32101 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    32161 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    32221 acaaagcagg ttacaatctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    32281 cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    32341 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    32401 aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    32461 tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    32521 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    32581 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    32641 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    32701 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    32761 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    32821 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    32881 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    32941 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    33001 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    33061 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    33121 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    33181 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg gctatgcatg
    33241 aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc gagcactatc
    33301 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    33361 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga
    33421 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    33481 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    33541 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac
    33601 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    33661 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    33721 aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca
    33781 atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg
    33841 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    33901 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    33961 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    34021 taatttttta aatattattt tcccatgaat aaaattggag taagtgcgtg gatagtaata
    34081 atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa
    34141 ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag
    34201 ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc
    34261 agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc
    34321 ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg
    34381 gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc
    34441 cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact cactatccgc
    34501 agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa
    34561 ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt
    34621 tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct ttagaagatg
    34681 acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg caaaatttca
    34741 gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat ttcaccagct
    34801 acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca
    34861 gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct tttccgtcct
    34921 taaatgagga gcaaatagat ttctctatcc actacaacaa attcatcctg cgtgatccca
    34981 tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca
    35041 gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc
    35101 gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag
    35161 ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac
    35221 acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat
    35281 aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag
    35341 ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc
    35401 gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg
    35461 gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt
    35521 tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg
    35581 aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac
    35641 cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac tgccattcgg
    35701 gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg tcgaatggat
    35761 cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa cctggaaaac
    35821 gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc gctgaagctg
    35881 ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtgggt
    35941 ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg
    36001 acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt
    36061 tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc
    36121 catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaagga
    36181 atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt
    36241 tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
    36301 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
    36361 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
    36421 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg
    36481 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
    36541 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
    36601 gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt
    36661 gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
    36721 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
    36781 gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
    36841 cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca
    36901 ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg ccagctctgg
    36961 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg
    37021 agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag
    37081 atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca gcatattcca
    37141 tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca tgttacgttt
    37201 actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga
    37261 tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa acagcgatac
    37321 gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac
    37381 agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa
    37441 aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg
    37501 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtag
    37561 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc
    37621 tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga
    37681 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
    37741 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
    37801 gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc gcactggcat
    37861 tctggctgtt ttcggcctca accgtacact aaagaccaaa aaaacccgct gattaagcgg
    37921 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
    37981 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
    38041 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
    38101 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
    38161 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt atcagtacac caattgtggt
    38221 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgttg
    38281 cttattttag ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
    38341 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
    38401 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
    38461 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
    38521 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
    38581 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
    38641 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
    38701 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
    38761 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc ctctcatctg cgttagcaaa
    38821 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
    38881 cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta
    38941 caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct gctctattta
    39001 tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc gtcgttttct
    39061 gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa tgcaatcaag
    39121 gaaaaattat tttatttttt taacgagata caacaatcat cttaacgaag tatataatat
    39181 atatcgtcaa ccatgctcat attagattat tttttgagcg cacagccgca gctaacacca
    39241 ttgcaattaa caaatttgca tcaatccacc atcaatttac acacattatt atgtgataat
    39301 tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact tcaatgtctt
    39361 aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg catatttttt
    39421 ccttaagctt taggaagttt ttattattta ctttgggcct ggagacagga aaaattatgc
    39481 tgacattcat tgaactcctt attggggttg tggttattgt gggtgtggct cgctacatca
    39541 ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg cctgttattg ctgattatca
    39601 gtgccattat ggggcacaaa gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca
    39661 cggatatcgt tgaatacgtt aaaatattac taatgagccg cggcggcgac ctcggcatga
    39721 tgattatgat gctgtgtggc tttgccgctt acatgaccca tatcggcgcg aatgatatgg
    39781 tggtcaagct ggcgtcaaaa ccattgcagt atattaactc cccttacctg ctgatgattg
    39841 ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc ttccgcaacc ggtctgggtg
    39901 ttttgctgat ggcaaccctg tttccggtga tggtaaacgt tggtatcagt cgtggcgcag
    39961 cagctgccat ttgtgcctcc ccggcggcga ttattctcgc accgacttca ggggatgtgg
    40021 tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc
    40081 ctatctcaat tgctgcaatt atcggcatgg cgatcgccca cttcttctgg caacgttatc
    40141 tggataaaaa agagcacatc tctcatgaaa tgttagatgt cagtgaaatc accaccactg
    40201 cccccgcgtt ttatgccatt ttgccgttca cgccgatcat cggtgtactg atttttgacg
    40261 gtaaatgggg tccgcaatta cacatcatca ctattctggt gatttgtatg ctgattgcct
    40321 ccattctgga gttcctccgc agctttaata cccagaaagt tttctctggt ctggaagtgg
    40381 cttatcgcgg gatggcagat gcgtttgcta acgtggtgat gctgctggtt gccgctgggg
    40441 tattcgctca ggggcttagc accatcggct ttattcaaag tctgatttct atcgctacct
    40501 cgtttggttc ggcgagtatc atcctgatgc tggtattggt gattctgacc atgctggcgg
    40561 cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac
    40621 tggcgcactc ttccggcatt aacccggcgt atttgactat cccgatgctg caggcgtcaa
    40681 acctgggtcg taccctatca cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga
    40741 aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc ggtgcttgtt gggctagtga
    40801 ttgttatcgt tgctacagag ctgatggtgc caggaacggc agcagcggtc acagacaagt
    40861 aaatagtaat gccggaggca agtttcctcc ggctttatca tgtcacccac tgtggtatat
    40921 gcgttgtggt ctgccaactt tgccgtgaac aatttcagca ataatcagat ggcggctggc
    40981 gcaatattca agataacgcc tggcagtggt gcggctgatg gtcagtgcct gcgccaccgt
    41041 ttctgccgta tgttgcacac caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa
    41101 ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg cgagcatagg cattaaacat
    41161 ctcatcaatt tgcttctggc tggcgctatc aatactttcc agcatatgtt tacgctggcg
    41221 gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg aagtacgaaa aaaccgggag
    41281 aacattggat tattcgggat ctgatgggat tagatttggt ggggcttgca agcctgtagt
    41341 acaaatttta ggcgttaacc aatgaaacgc gaaagatagt aaaaaattgc ttttgtttca
    41401 ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa tcatagcttc aaaaaacgtt
    41461 caaaaccgat aacaaccagg ctgtcaccaa atgactcata tcacaaatca gctcatgccg
    41521 tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg gctcatggtt
    41581 gttatacggc ttttttcgca tcttgtggtt ccaacaaagg agctacaggc tcttcctgtt
    41641 tatgaacacc atacattgac aaaatctctt ggcgagtttc tggaggtaat ttatgcagcg
    41701 ccaaagctgt ttgtagtgtt tggcgatcaa tacccagcgt tgccaatagg gtgtcaacgg
    41761 agatggaggg gcgttcttgc attaatccgg aaagcccata tttagccatg tttagctcta
    41821 tctctcggac ttcattttct cctagtttat tgattaacag ggagaacatt ttcaatgtat
    41881 cagcattcat ttcttcgata gcaatttttt gcatagccgg tattgccggg atatattcaa
    41941 ccccaggccc gcgacgccct tcttgtgaac gcttcggcca tccttctttc tttgctcgtt
    42001 tatgcacccc ctgtattgtc ccaggcattc catccagttc agtaatttgt ttgggtgtca
    42061 tccactggtt tatctcattt gcgcttttca taaactaagt ttaccagatt tatctatata
    42121 aaaatcaatg gattacaaaa taaaccacat taactgatat aaaccggttt tgtttatttg
    42181 gtaatgtata aaacagataa tcaagacgaa ttatttgttt tggtaaccct ttatcatacg
    42241 caatatggtt ggtgtgcgaa agatgagtaa cgattggcat cgggccgacg tgattgcggc
    42301 cctcaaaaaa acaggaaccc ctctggcaaa attgtccaga cgttcaggtt attcaaccgg
    42361 gacgcttgca aatgcattag tcaggccatg gcctaaaggt gaacgcatta ttgctgacgc
    42421 cttaaacatg aaacctgagg aaatttggcc tagtagatat ctaagggatc acaatgagag
    42481 agtggatatc agcaattgaa tgcatgggtt tatctggttt acctggttca ttaatggggg
    42541 ttcacaaaag agctcagaga gaaggatggc tcaagagaag aagagaagga attagagggg
    42601 ttgcttacga ataccatata tcttctcttc ctaacattgc tcgagctgaa gttttgctcc
    42661 aacaaggaaa atttaaaacc agtcagggat gttttgaaat cgcccgcccc acgctggaag
    42721 ctcatgatta tgatcgtgag gcactatgga gcaaatggga taaagccagc gattcccagc
    42781 gcagcctggc tgaaaaatgg ttgccctcaa ttcaggccac agacgaaatg ctgaaccagg
    42841 ggatttcaac gaaaacggct tttgcgaccg ttgcagggca ttaccaggtc agcgcatcca
    42901 ctttgcggga caagtattac caggtacaga agtttgcgaa gcctgactgg gcggctgcac
    42961 ttgttgatgg acgtggagca tcccgtagca atgttcacaa aagtgaattt gacgaggatg
    43021 cctggcagtt tctgattgca gattatctgc gaccggaaaa acccgccttc cgcaaatgtt
    43081 atgagcgtct ggaactggca gcccgcgagc atggctggag tattccctcc cgtgccacgg
    43141 cctttcgccg gattcagcaa ctggacgagg caatggttgt tgcctgtcgt gaaggtgaac
    43201 atgcactgat gcatctgata ccggcacagc agcgaactgt ggaacacctg gacgccatgc
    43261 agtggatcaa cggcgacggt tatctgcata acgtctttgt acgctggttt aacggtgatg
    43321 tgatccgtcc gaaaacatgg ttctggcagg atgtgaaaac ccgaaaaatt ctgggctggc
    43381 gctgcgatgt gagcgagaac attgattcaa ttcgcctctc gttcatggat gttgtgactc
    43441 gctacagtat cccggaggat tttcacatca ccattgataa cacccgtggt gctgcgaata
    43501 aatggctgac gggaggcgcg cccaatcgct accgctttaa ggtaaaagag gacgatccaa
    43561 aaggactgtt tttactgatg ggtgcgaaaa tgcactggac aagcgttgtt gccggtaaag
    43621 gctggggcca ggcaaaacct gttgaacgtg ctttcggtgt tggtgggctt gaggaatacg
    43681 ttgataagca tccggcactg gctggcgcat atacggggcc aaatccgcag gcaaaacctg
    43741 ataactatgg cgaccacgct gttgatgcag agctgtttct gaaaaccttt gccgaaggtg
    43801 tggcgatgtt caatgccaga acaggccgtg aaacagaaat gtgcgggggc aaactctcgt
    43861 ttgatgatgt tttcgagcgt gaatacgcca gaacgattgt gcgtaagcca accgaagaac
    43921 aaaaacggat gctgttactg cctgccgagg cggtgaacgt ttcacgcaaa ggcgagttta
    43981 cgcttaaagt tggcggctcc cttaaaggcg cgaaaaacgt ttattacaac atggcattaa
    44041 tgaatgccgg agtgaaaaaa gttgtggtca gatttgatcc acagcagtta cacagcacgg
    44101 tttattgag
//
LOCUS       DADLDT020000019        43854 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000019 DADLDT020000000
VERSION     DADLDT020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43854
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.019"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(16..603)
                     /gene="mog"
                     /locus_tag="JQH22_000997"
     CDS             complement(16..603)
                     /gene="mog"
                     /locus_tag="JQH22_000997"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFB2205400.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(718..1671)
                     /gene="tal"
                     /locus_tag="JQH22_000998"
     CDS             complement(718..1671)
                     /gene="tal"
                     /locus_tag="JQH22_000998"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFB2205401.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1950..3379
                     /locus_tag="JQH22_000999"
                     /pseudo
     CDS             1950..3379
                     /locus_tag="JQH22_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3449..4225
                     /gene="yaaA"
                     /locus_tag="JQH22_001000"
     CDS             3449..4225
                     /gene="yaaA"
                     /locus_tag="JQH22_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFB2205402.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4378..4674)
                     /locus_tag="JQH22_001001"
     CDS             complement(4378..4674)
                     /locus_tag="JQH22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFB2205403.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4888..6174)
                     /gene="thrC"
                     /locus_tag="JQH22_001002"
     CDS             complement(4888..6174)
                     /gene="thrC"
                     /locus_tag="JQH22_001002"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFB2205404.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6175..7107)
                     /gene="thrB"
                     /locus_tag="JQH22_001003"
     CDS             complement(6175..7107)
                     /gene="thrB"
                     /locus_tag="JQH22_001003"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFB2205405.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7109..9571)
                     /gene="thrA"
                     /locus_tag="JQH22_001004"
     CDS             complement(7109..9571)
                     /gene="thrA"
                     /locus_tag="JQH22_001004"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFB2205406.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9597..9716)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9652..9717)
                     /gene="thrL"
                     /locus_tag="JQH22_001005"
     CDS             complement(9652..9717)
                     /gene="thrL"
                     /locus_tag="JQH22_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFB2205407.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9931..10617)
                     /locus_tag="JQH22_001006"
     CDS             complement(9931..10617)
                     /locus_tag="JQH22_001006"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFB2205408.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11017..11157)
                     /locus_tag="JQH22_001007"
     CDS             complement(11017..11157)
                     /locus_tag="JQH22_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205409.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11253..11969
                     /gene="arcA"
                     /locus_tag="JQH22_001008"
     CDS             11253..11969
                     /gene="arcA"
                     /locus_tag="JQH22_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFB2205410.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12028..13380)
                     /gene="creD"
                     /locus_tag="JQH22_001009"
     CDS             complement(12028..13380)
                     /gene="creD"
                     /locus_tag="JQH22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFB2205411.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13438..14862)
                     /gene="creC"
                     /locus_tag="JQH22_001010"
     CDS             complement(13438..14862)
                     /gene="creC"
                     /locus_tag="JQH22_001010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFB2205412.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14862..15551)
                     /gene="creB"
                     /locus_tag="JQH22_001011"
     CDS             complement(14862..15551)
                     /gene="creB"
                     /locus_tag="JQH22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFB2205413.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15564..16037)
                     /gene="creA"
                     /locus_tag="JQH22_001012"
     CDS             complement(15564..16037)
                     /gene="creA"
                     /locus_tag="JQH22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFB2205414.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16248..17117
                     /gene="robA"
                     /locus_tag="JQH22_001013"
     CDS             16248..17117
                     /gene="robA"
                     /locus_tag="JQH22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFB2205415.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17114..17761)
                     /gene="gpmB"
                     /locus_tag="JQH22_001014"
     CDS             complement(17114..17761)
                     /gene="gpmB"
                     /locus_tag="JQH22_001014"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFB2205416.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17813..18333
                     /gene="yjjX"
                     /locus_tag="JQH22_001015"
                     /pseudo
     CDS             17813..18333
                     /gene="yjjX"
                     /locus_tag="JQH22_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18418..18744)
                     /gene="trpR"
                     /locus_tag="JQH22_001016"
     CDS             complement(18418..18744)
                     /gene="trpR"
                     /locus_tag="JQH22_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFB2205417.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18834..20771)
                     /gene="sltY"
                     /locus_tag="JQH22_001017"
     CDS             complement(18834..20771)
                     /gene="sltY"
                     /locus_tag="JQH22_001017"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFB2205418.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20982..22649
                     /gene="ettA"
                     /locus_tag="JQH22_001018"
     CDS             20982..22649
                     /gene="ettA"
                     /locus_tag="JQH22_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFB2205419.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22756..22923)
                     /locus_tag="JQH22_001019"
     CDS             complement(22756..22923)
                     /locus_tag="JQH22_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205420.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22957..24189)
                     /gene="nadR"
                     /locus_tag="JQH22_001020"
     CDS             complement(22957..24189)
                     /gene="nadR"
                     /locus_tag="JQH22_001020"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFB2205421.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24210..25592)
                     /gene="radA"
                     /locus_tag="JQH22_001021"
     CDS             complement(24210..25592)
                     /gene="radA"
                     /locus_tag="JQH22_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFB2205422.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25641..26609)
                     /gene="serB"
                     /locus_tag="JQH22_001022"
     CDS             complement(25641..26609)
                     /gene="serB"
                     /locus_tag="JQH22_001022"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFB2205423.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26715..27359
                     /locus_tag="JQH22_001023"
     CDS             26715..27359
                     /locus_tag="JQH22_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFB2205424.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27387..28414
                     /gene="lplA"
                     /locus_tag="JQH22_001024"
                     /pseudo
     CDS             27387..28414
                     /gene="lplA"
                     /locus_tag="JQH22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28446..28709
                     /locus_tag="JQH22_001025"
     CDS             28446..28709
                     /locus_tag="JQH22_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2205425.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28870..29589)
                     /gene="deoD"
                     /locus_tag="JQH22_001026"
     CDS             complement(28870..29589)
                     /gene="deoD"
                     /locus_tag="JQH22_001026"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFB2205426.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29646..30869)
                     /gene="deoB"
                     /locus_tag="JQH22_001027"
     CDS             complement(29646..30869)
                     /gene="deoB"
                     /locus_tag="JQH22_001027"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFB2205427.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30921..32243)
                     /gene="deoA"
                     /locus_tag="JQH22_001028"
     CDS             complement(30921..32243)
                     /gene="deoA"
                     /locus_tag="JQH22_001028"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFB2205428.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32370..33149)
                     /gene="deoC"
                     /locus_tag="JQH22_001029"
     CDS             complement(32370..33149)
                     /gene="deoC"
                     /locus_tag="JQH22_001029"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFB2205429.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33407..34948
                     /locus_tag="JQH22_001030"
     CDS             33407..34948
                     /locus_tag="JQH22_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFB2205430.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVGILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34920..35783
                     /locus_tag="JQH22_001031"
     CDS             34920..35783
                     /locus_tag="JQH22_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFB2205431.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35997..36779)
                     /locus_tag="JQH22_001032"
     CDS             complement(35997..36779)
                     /locus_tag="JQH22_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFB2205432.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36776..37849)
                     /locus_tag="JQH22_001033"
     CDS             complement(36776..37849)
                     /locus_tag="JQH22_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFB2205433.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37971..38132)
                     /locus_tag="JQH22_001034"
     CDS             complement(37971..38132)
                     /locus_tag="JQH22_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFB2205434.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38249..38854)
                     /gene="osmY"
                     /locus_tag="JQH22_001035"
     CDS             complement(38249..38854)
                     /gene="osmY"
                     /locus_tag="JQH22_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFB2205435.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39247..40836)
                     /gene="prfC"
                     /locus_tag="JQH22_001036"
     CDS             complement(39247..40836)
                     /gene="prfC"
                     /locus_tag="JQH22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFB2205436.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40927..41604)
                     /gene="yjjG"
                     /locus_tag="JQH22_001037"
     CDS             complement(40927..41604)
                     /gene="yjjG"
                     /locus_tag="JQH22_001037"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFB2205437.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41619..42065)
                     /gene="rimI"
                     /locus_tag="JQH22_001038"
     CDS             complement(41619..42065)
                     /gene="rimI"
                     /locus_tag="JQH22_001038"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFB2205438.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42034..42447)
                     /gene="holD"
                     /locus_tag="JQH22_001039"
     CDS             complement(42034..42447)
                     /gene="holD"
                     /locus_tag="JQH22_001039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFB2205439.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42550..43581
                     /gene="rsmC"
                     /locus_tag="JQH22_001040"
     CDS             42550..43581
                     /gene="rsmC"
                     /locus_tag="JQH22_001040"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFB2205440.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        10323 a        11990 c        11101 g        10440 t
ORIGIN      
        1 ctcgggggga tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc
       61 aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac
      121 gctggcaaaa ataccatgca ccacaacgtt accctcagcg tccttcacac cttccagcgt
      181 ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac
      241 gcccacctga cgcgaaagga tcgcagttgg tacaaaatgc aggctgatct ggcgcatctg
      301 ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc
      361 acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac
      421 cagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag
      481 ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgctggaa tgcctttatc
      541 ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt
      601 catcaagatt tttccgttta aacaatctcg ttaaacagaa tgatacactg cgaagggagt
      661 gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta
      721 cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc
      781 ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc
      841 agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc
      901 cccttcgctc tccgccagct ctttcagcag tgccggtgcg atggtcagac ggtcacagcc
      961 tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata
     1021 accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc
     1081 cggagcgtac tctttcttat cggtattcgc tttgtaccag tcaagaatac ggccaacaaa
     1141 cggcgagatc aggaacacgc ccgcctccgc acaagcacga gcctgagcga aggagaacag
     1201 cagggtcagg ttacagttga taccttcttt ttccagctgt tctgcagcac ggataccctg
     1261 ccaggtagaa gccagtttga tcagaatacg atcgttgcta atacctgcat cgttgtagag
     1321 tttgatcagg cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc
     1381 aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc
     1441 cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca
     1501 ggcgacagca tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag
     1561 agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt
     1621 gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt
     1681 tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca
     1741 acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga
     1801 cactgcgttg atatcgccca ccatttttat acaaaacctc atgtatgcta cgcagaagtt
     1861 atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt
     1921 catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg
     1981 tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc
     2041 gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca
     2101 ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc
     2161 gcgtgggtag cggcaacctg gccggcgttg cgctggcaat taccgccggt ggaccaggtg
     2221 ccgtcttttg gatgtgggtt gctgcgttta tcggcatggc gacctcgttt gccgaatgtt
     2281 cccttgcaca actttataaa gaacgtgacg tcaatgggca gtttcgtggc ggaccggcat
     2341 ggtatatggc gcgcgggctg gggatgcgct ggatgggcgt tctgttcgcc gtctttttgc
     2401 tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga
     2461 gtttttcttt tgattttcct ccgctggtga cgggcattat tctcgctgtc tttgctctgc
     2521 tggcaatcac tcgcggtctt catggcgtcg cccggctcat gcaggggttt gtcccgttga
     2581 tggcgataat ctgggtactg accagcctgg tgatttgcgt aataaatatc gggcaacttc
     2641 cccacgtcat ttggtctatt tttgagagtg cttttggctg gcaggaagcg gcaggcggcg
     2701 cggcgggata taccttaagc caggcgatta ctaacggttt tcagcgcagt atgttttcca
     2761 atgaggtggg aatgggatcg acgccaaacg cggcagcggc agctgcatcc tggcctccgc
     2821 atccggcagc acaagggatt gtccagatga ttggcatttt tatcgacacc ctggtcatct
     2881 gtacggcaag cgccatgctg atattactgg cgggtaacgg cacaacctac atgccgctgg
     2941 aaggtattca gcttatccag aaggcgatgc gggtgctaat gggttcctag ggtgctgaat
     3001 ttgttaccct cgtggttatt ctgtttgcct tcagctccat cgttgccaac tacatttatg
     3061 ccgaaaacaa tctcttcttt ttacgcctga acaaccctaa agcgatctgg tgtttgcgga
     3121 tctgcacctt cgcaacgatc atcggcggca ccttgctaag ccttccgctg atgtggcaac
     3181 tggcagatat ataatggcct gcatggctat taccaatttg accgccattt tactgctctc
     3241 gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
     3301 ggtgtttgat ccgttgcgtt atccggatat cggtcgccaa ctttctccgg acgcgtggga
     3361 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
     3421 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
     3481 tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc
     3541 ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
     3601 catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt
     3661 cacgccggcg aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct acaccggctt
     3721 gcaggccgag accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
     3781 ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat
     3841 ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat
     3901 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
     3961 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
     4021 ggtgttcctc gatgagaaga acggcaaatt taagatcatc agcttctacg ctaagaaagc
     4081 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
     4141 tggttttaat agcgaaggtt acttctttga tgaagattcc tccagcaatg gcgaactggt
     4201 gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct
     4261 tatccggcct acaaaagcat gcagattcaa tatattgcag aattaacgta ggcctgataa
     4321 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
     4381 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg
     4441 gcgcggcggt gacggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
     4501 tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg
     4561 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat
     4621 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
     4681 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg
     4741 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgataat ttctcctttt
     4801 tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc
     4861 cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc
     4921 agaaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc
     4981 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
     5041 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac gcagcgcacg
     5101 atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt
     5161 ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt
     5221 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
     5281 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac
     5341 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga atgacttcgc
     5401 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
     5461 gcgcgcctcc tgcggcagct gcgcaacagc ttcaaagtag tagcaaatct gcgccagcaa
     5521 acggctgatg ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc
     5581 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
     5641 ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg
     5701 tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgcagc
     5761 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcatccg caatatgggt
     5821 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
     5881 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
     5941 cgcgcgcacg cgctcttcca ggatttcctg tgggatttca tcaccaataa acgccgagag
     6001 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
     6061 gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
     6121 ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt
     6181 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
     6241 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
     6301 tagagcgaac aaggtcgggc cggagccgga gataccgctc actaccgcgc cgatttccgc
     6361 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat
     6421 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
     6481 gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
     6541 agcccgggct tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc
     6601 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
     6661 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag
     6721 ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccgcagt gttcattcat
     6781 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac cgatcggcat
     6841 attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc aaaaacgctc
     6901 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
     6961 gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
     7021 accatcaacg ggtgccaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
     7081 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
     7141 gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
     7201 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
     7261 ctttgaacag cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt
     7321 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
     7381 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg
     7441 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
     7501 ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc
     7561 gcgggtccgg ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca
     7621 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
     7681 tcaccaattc atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
     7741 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
     7801 gatgatagta atccatcgac gaggtgttgg cctttttgtt cggcgtaaca acgtggaaac
     7861 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
     7921 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
     7981 gctctttggc ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag
     8041 tgagcagagc cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca
     8101 gccagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
     8161 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
     8221 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
     8281 caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
     8341 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccgtcaccg
     8401 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
     8461 cagctcgcac acagtcgctt tgtggaacgc agaaactgat actgtattcg gaagatgatt
     8521 gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg cgcgccgcca
     8581 tgccaaccat ccctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
     8641 tggaaatgcc cttgaccggt aattcgtctt catcacggct ggcaccaatg agcgtacctg
     8701 gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
     8761 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
     8821 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc
     8881 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
     8941 cggagtagtc ggaaccgtta cgtccaagca ccaccagttc gcctttttca ttaccggcgg
     9001 tgaaacctgc catcaacacc atgtgatcag ccggaatgcg gcttgccgca atacggcggg
     9061 tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagc agtttttcga
     9121 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
     9181 tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg cactgcccca
     9241 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctgatcg acgaaagttt
     9301 tcaattgcgc cagcgggaac cccggctggg cggcggcgag tcccgtcaaa agttcggcaa
     9361 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
     9421 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
     9481 cctgcctggc attgctttcc agaatatcgg caacacgcag aaaacgttct gcatttgcca
     9541 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
     9601 aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc
     9661 accgttacct gtggtaatgg tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga
     9721 tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg cctatattgg ttaaagtatt
     9781 tagtgaccta agtcaataaa attttaattt actcacggca ggtaaccagt tcagaagctg
     9841 ctatcagaca ctcttttttt aatccacaca gagacatatt gcccgttgca gtcagaatga
     9901 aaagctgaaa aatacttact aaggcgtttt ttatttggtg atattttttt caatatcatg
     9961 cagcaaacgg tgcaacattg ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa
    10021 ccagtcgacc agttttatgt catctgccac tgccagagcc gtcagcaatg ccatgactcg
    10081 ttcgcgtaaa gcttgcagtt gatgttggtc tgccgttgtg tcacttttcg ccggttgttg
    10141 tattaatgtt gctaattgat agcaatagac catcaccgcc tgccccagat tgagcgaagg
    10201 ataatccgcc accatcggca caccagtaag cacgtcagcc aacgctaact cttcgttagt
    10261 cagcccggaa tcttcgcgac caaacaccag cgcggcatgg ctcatccatg aagatttttc
    10321 ctctaacagc ggcaccagtt ctactggcgt ggcgtagtaa tgatatttcg cccgactacg
    10381 cgcagtggtg gcgacagtga aatcgacatc gtgtaacgat tcagccagtg tcgggaaaac
    10441 tttaatatta tcaataatat caccagatcc atgtgcgacc cagcgggtgg ctggctccag
    10501 gtgtgcctga ctatcgacaa tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc
    10561 cgctgcccca atattttctg ctctggcggg tgcgaccagg ataatcgtta tacgcatatt
    10621 gccactcttc ttgaccaaat aaccgcgaac agggtgatca ctgtcaactt attacgcggt
    10681 gcgaatttac aaattcttaa cgtaagccgc agaaaaagcc ctttacttag cttaaaaaag
    10741 gctaaactat tttctgactg cactaacggt ttaattgtta aaaaatgcta catatccttc
    10801 tgtttactta ggataatttt ataaaaaata aatctcgaca attggattca ccacgtttat
    10861 tagttgtatg atgcaactag ttggattatt aaaataatgt gacgaaagct agcatttaga
    10921 tacgatgatt tcatcaaact gttaacgtgc tacaattgaa cttgatatat gtcaacgaag
    10981 cgtagtttta ttgggtgtcc ggcccctctt agcctgttat gttgctgtta aaatggttag
    11041 gatgacagcc gtttttgaca ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat
    11101 gatgttgttg acgttgatgg aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt
    11161 acgccgatca tgttaatttg cagcatgcat caggcaggtc agggactttt gtacttcctg
    11221 tttcgattta gttggcaatt taggtagcaa acatgcagac cccgcacatt cttatcgttg
    11281 aagacgagtt ggtaacacgc aacacgttga aaagtatttt cgaagcggaa ggctatgatg
    11341 ttttcgaagc gacagatggc gcggaaatgc atcagatcct ctctgaatat gacatcaacc
    11401 tggtgatcat ggatatcaat ctgccgggta agaacggtct tctgttagcg cgtgaactgc
    11461 gcgagcaggc gaatgttgcg ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa
    11521 ttctcggcct cgaaatcggt gcagatgact acatcaccaa accgttcaac ccgcgtgaac
    11581 tgacgattcg tgcacgcaac ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag
    11641 aacgtcgtag cgttgaaagc tacaagttca atggttggga actggacatc aacagccgtt
    11701 cgttgatcgg ccctgatggc gagcagtaca agctgccgcg cagcgagttc cgcgccatgc
    11761 ttcacttctg tgaaaaccca ggcaaaattc agtctcgtgc tgaactgctg aagaaaatga
    11821 ccggccgtga gctgaaaccg cacgaccgta ctgtagacgt gacgatccgc cgtattcgta
    11881 aacatttcga atctacgccg gatacgccgg aaatcatcgc caccattcac ggtgaaggtt
    11941 atcgcttctg cggtgatctg gaagattaat cggctttacc accgtcaaaa aaacggcgct
    12001 ttttagcgcc gtttttattt ttcaacctta tttccagata cgtaattcat cgtccgttgt
    12061 aacttcttta ctggctttca ttttcggcag tgaaaacgca taccagtcta tattacgagt
    12121 cacaaacatc atgccggcca gcgccaccac cagcacactg gttcccaaca acagcgcgct
    12181 atcggcagag ttgagcagtc cccacatcac tccatccagc aacaacagcg cgaggataaa
    12241 caacatgcta ttgcgccagc ctttcaatac cgcctgcaaa taaataccgt tcattaacgc
    12301 cccaatcaga ctggcgatta tccatgccac ggtaaaaccg atatgttcag aaagcgccag
    12361 caagagcaaa taaaacatca ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa
    12421 acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata aaagtcagtg caatcagcag
    12481 aatggcgtac ttagtcgccc ggtccgttaa ttggtattga tcggctggcg tcgtcactgc
    12541 gacgctaaac gcggggaagt tttcccagcc ggtatcattg cctgaagcaa aacgctcacc
    12601 gagattatta gcaaaccagc tgctttgcca ttgcgcctga aaacctgact cgctagcttc
    12661 ccgtttggct ggtagaaaat cacctaaaaa actgggatgc ggccagttgc tggttaaggt
    12721 catttcgcta ttacgcccgg caggcaccac agaaagatcg ccggtaccgc ttaaattcag
    12781 tgccatattc agctgcaggt tctgcttacg ccagtcccct tcgggtaaag ggatatgcac
    12841 gccctgcccg ccttgctcta acccggtacc gggttcaatg gtcagtgccg ttccgttaac
    12901 ttcaggcgct ttcaccacac caataccacg cgcatccccg acgctaatca caataaaggg
    12961 cttgcctaag atgatatttg gcgcgttgag ctcgctaaga cgcgaaacat cgaaatcggc
    13021 ttttagcgtt aaatcactgt gccagacctg accggtataa atccctatct tgcgttcttc
    13081 cacgttctga ttgccatcaa ccatcaatga ctcaggtaac aaaaaatgga taaaacttcg
    13141 tttccgctcc acggttttat cctcttcctg caccgtataa agctcggtca caggaatagc
    13201 gataagcggc ccaacgagtt tttgcggccc gctggtactt tggcgaatcg catcttccac
    13261 atcgctacgg taatcggcac gttcgacaat cacttgccga atcagcatta tcggaatcaa
    13321 caacagcaat actgcaccaa acaaggtggt cattttccag aacagggggg atttcaacat
    13381 agtcttctcc tttgcaatgg cagcaggata cgaaggctat gtgggaagaa tttgaagcta
    13441 tgtgaagtga cggtgaagtc gtagcgaggc cagcacgcca ccttcctgca cgttgcgcag
    13501 cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg aacgccaacc ccagaccgct
    13561 gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc
    13621 gtagtcagga ataccactac cggtatccag caccttaagc gtgacgtgtt cctgatccac
    13681 ttcggcgctt agcgttatgc aaccgctctc gggagtaaaa tcgatggcgt tatccagtaa
    13741 attccccagc gcctgatcca gtaacgccgg ttcagcagca acattaacct cggtgggcat
    13801 aacatgcaaa gtgatgtttt tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg
    13861 aaataatgcc gccacatcaa cagcagtcag aacgacttcc tgacgattct ccagtcttgc
    13921 ctggcgtagt aacgtttcca ccagcgcctg cattcgcgca ttttgtgtca gaatgttgtc
    13981 agtaaaacga gccactactt caggcggcgg accttcgcgt aaaatttccg ccgcgccacg
    14041 aatcgccgcc agtgggcttt ttagctcatg ggttaacgca taaacatact gctcaatata
    14101 gtttttccct tccagcttca cgcgcatact ttccagcgcc tgcgcgagtt tacgcagctc
    14161 gctactaccg agatcgggaa gaggaacggg cttattgtca gtgacggaat cagcatagcg
    14221 agtgagccgg gcaatggagc ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc
    14281 aatccccaac aaaatggcgc tggcccataa aattcgccgc tcgctgcgtt taatgaccgg
    14341 agccatcgcc gcgttcggtt tgcctacgct caaaacgcca ataagccgcg agccgcccat
    14401 aatcggcgcg gcaacataca tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct
    14461 gcgcgcacca tactgaccac gcaacgttag ccagacgtca ttccagcgcg aataatcctg
    14521 tccaacggct ttatttgccg aatcgaacaa tactttgcca tgcgcatcgg tcatatagac
    14581 atgatattca ttgcgcactt tgttaatgcc accaatattg gcgcgaaacg ggcgatgttg
    14641 tagctgatta aacgcctgcg ccagttgccc atgcgttggg tccccagaga gcaaatcggg
    14701 acgcgccagc tccgcaagta acgttgccgt gtcgatcaac gtcccctccg ttgctcttcg
    14761 cacgcccggt ttaacttctt tgacaaaaat agccagtacg aaccaggccg ccaccgccac
    14821 cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc attacaagcc cctcaggcta
    14881 tatcccatgc cgcgatgagt attaatcggt gaaagatcgg ggttgatggc gcgcagcttg
    14941 gcacgcagcg ttttaatgtg gatatcgacg gtgcgatcgt aggtgtcctg cgcatcttcc
    15001 cacacgctat ccattagttg ctggcgggac cagacgcggc ctggcgactt gagtaacgtc
    15061 ttcagcaata aaaactcata ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc
    15121 gccgcgggtt cattcaattc aaaatgacca atacggatga cgggagacgg cgtcgagaac
    15181 ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca cttcgcgggg tgaaaacggt
    15241 ttagccacat agtcgtcagc gccaatttcc agcccaagca ggcgatcgac ctcttcactt
    15301 cgggccgtca ggaacagtac aggtaacgcc ggatggagcg ccagtaactg gcggcacaat
    15361 tcaaagccac tgatatcagg caaaccaacg tcgagaatca tgacgtcggg aacctgctgg
    15421 cgagctttat ccagcaccgg caaacctcgc tcaaagacct cgacggcaaa accttcctgc
    15481 tgcaacatgt agaccagcgt gtcggctatc ccttgctcat cttccactaa ccagaccgtt
    15541 tcccgttgca taaatcgcct ctgttattgc cgccacggca tgacaggaac cgcgctaatc
    15601 gcgtttttcg gcgaaccttc tacaactttg tcggagtaag ccagataagc gagcgcgttg
    15661 cgtttggcgt cataaaagcg cacgacctgt aacgacttaa agaccaggga cgtgcgtttt
    15721 ttgaatacta cctcgccctg agctttgccg tttttaatac gatccgacag ttcaatcggc
    15781 ccgacttgct gacaagaaat ggccgcatcg gaggtatctt ctgccaggcc caatccccct
    15841 ttaataccac cggttttcgc ccggctcaca taacaggtga catttttcac atcgggatcg
    15901 tcaaaggctt ccacaacaat tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc
    15961 gaaccaatct cttctgcatg agccaatggc cccagcatta tcaggcttaa agacaggatc
    16021 aaatgcttgt atttcatatt gttaccattt ttatctatta cacgagcaat tagttcgtca
    16081 cggtaaataa catattgatt aagaacaaaa tctcaattct tttatttccg tcaagcccta
    16141 aaacatactc tactaagaaa aaaacactga atgctaaaac agcaaaaaat gctattatcc
    16201 aattacctga tgtcaggtgc tcgttgttga aaggatgagg atattttatg gatcaggccg
    16261 gcattattcg cgacctttta atctggctgg aaggtcatct ggatcagccc ctgtcgctcg
    16321 acaatgtagc ggcgaaagca ggttattcca agtggcactt acagagaatg tttaaagatg
    16381 tcactggcca tgctattggc gcgtatattc gtgctcgtcg tttgtcgaaa tcggcggtcg
    16441 cactacgcct gactgcgcgt ccgattctgg acatcgcgct gcaataccgc ttcgactctc
    16501 aacagacatt tacccgcgca ttcaagaagc agtttgccca gactcctgca ctttaccgcc
    16561 gttctcctga atggagcgcc tttggtattc gcccgccgct acgtctgggt gaattcacaa
    16621 tgccagagca caaatttgtc accctggaag atacgccgct gattggtgtt acccagagct
    16681 actcctgttc gctggagcaa atctctgatt tccgccatga aatgcgttat cagttctggc
    16741 acgattttct cggcaacgcg ccgaccattc cgccggtgct ctacggcctg aatgaaacgc
    16801 gtccgagtca ggataaagac gacgaacaag aggtattcta taccaccgcg ttagcccagg
    16861 atcaggcaga tggctatgta ctgacggggc atccggtgat gctgcagggc ggcgaatatg
    16921 tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga gtttatcctg acggtatacg
    16981 gaacgtgcat gccaatgctc aatctgacgc gccgtaaagg tcaggatatt gagcgatact
    17041 acccggcaga agacgccaaa gcgggagatc gcccaattaa tctgcgctgt gaactgctga
    17101 ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg ggcgtccaga tgcgagatgt
    17161 cccctgcggt ttcaacgacc cagccagacg ccagccacag gctttcctga taatccacgc
    17221 gcgaaataga acagttacgc agacgtaagc ggcgctctac ccatgctggt aatccgagaa
    17281 tcgtactcac cagacatccc agtgcaatac catggctcac cagcagcggt cggcttccct
    17341 gcggtaagtc ccggcaagat tccagtgccg cattgacgcg atcgctgagc tcctgcattg
    17401 actcgccttc aggaatacgc ccgtcaacgg tgccattgac cagctgccga cgccagttct
    17461 cttcttcttc ggtcagagaa tcgatatgtc ttttttccag cacacccatg tttaattcac
    17521 gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg ggcgatgatt tccgccgtac
    17581 gccgggtgcg tcctaaatcg ctactgataa tatgcgtaat gccaagctct ttggcacggg
    17641 ttgccacctg catcgcctgt tgctcacctt tggcggtcag cgggctgtca gactggccct
    17701 gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg gactaggtat acctgtaaca
    17761 tgcttttttt ccgtatactg ctgtaacgtt aattttctga gtaatgctga ttatgcacca
    17821 agttgtctgt gcgaccacca atcccgctaa aattcaggcc attctgcagg catttcacga
    17881 gatcttcggc gaaggatcct gccatattgc atccgttgcc gtcgagagcg gtgtaccgga
    17941 acaacccttt ggcagtgagg aaacgcgcgc tggcgcacga aatcgggtag caaatgcccg
    18001 ccgtttactt ccagaggctg atttttgggt ggcgattgaa gctggcattg atggcgacag
    18061 cactttcagt tgggtggtga ttgaaaacgc cagccagcgc ggcgaggcgc gttcggcaac
    18121 tttgccgtta cctgcggtga ttctggaaaa agttcgtgaa ggcgaggcgc ttggtcccgt
    18181 gagtcgcgtt ataccggtat tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt
    18241 accgccggga aactcactcg cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt
    18301 ccgtttcaca atgccgtgta tcaggcattg taggacggat aaggcgttca cgccgcatcc
    18361 gacattcggt gcacgatgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca
    18421 atcgcttttc agcaacacct cttccagcca ctgacgcagc tccacgggcg cggctttcag
    18481 gctgttagat ccacgcgtaa tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg
    18541 ctgactcatt tcgccacgca acagctcttc gacaatacgc acgcgagtcc ccaacgcttc
    18601 gcgctcatct ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt tttggtaggc
    18661 attcttaagc aggtcgacaa aacgtaacca ctcctggtga cgctgttctg ccatcgctgc
    18721 tgaatagggt gattgttggg ccataatatg tcgccattgt tagcggggga agcaaaatga
    18781 ctcccccggt tgtactcgct aaagagtacg atagcatatc ataaacgtgc ggatcagtaa
    18841 cgacgtcccc attccgtggc gctcatcaac gtcggtttat cccccatgaa atagcggtag
    18901 taagcatcat aagccagcac gttcttcaca taaccgcgcg tctcggagaa cggaatactc
    18961 tcgacaaatg ccacagcgtc gatacgcccg gcgctgttgc caagccaggt tcgcacccgt
    19021 ccaggtccgg cgttataagc tgctgaggag aaaatacggt tattgccaaa ctgctgataa
    19081 acatattgca ggtaactggt gccaatgttg atgttcgttt ccggatccag caattgccca
    19141 gggctgctat aaccaggaat agagaacatc ttcaccgtat gggtcgctgt accaggcata
    19201 atctgcatca ggccgctggc ccctaccggt gatttcactt tcggattcca ggcgctctcc
    19261 tgacgggcaa tcgccatcgc atagctttgc gggatctctt tcccactggt gtaacgtttg
    19321 aaaagatcgt tgtaagccag cgggaatcgc tcttccagat gatcccaaag cttcccggcg
    19381 atcgttgcct gaacgctaag atcccaccat tggttattga aagcataccg agccagttga
    19441 gcctgctctg tttttgactt gctcttcacc agattggccc actcgctacg cgcggtatta
    19501 tcgagattcc agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg agtcagggtg
    19561 ctgtcaacat tctgcggcgc tttatcaatc ttcagctcat actcttcacc gatgcgttgt
    19621 gctgcaacca tcgggtagaa accacgctgt tgtatgagtt gatgcaaaat ctctttcgct
    19681 tcagcttcac gtccgcgttc cagcaataaa tccgcctgcc agtaacgcca ttcatctttc
    19741 tcttttgctt ccatcggcag acgcgccagc caggtattca ggccgcgacg atcgccggtg
    19801 ccaagcgcca ttcgcacacg gcgttcaatc aacgacgtgg attgcgagcg cataatggcg
    19861 tcatcgcgcc atttcgcctg ctcatcggtg acatcattgc ccatcaaacg ccaggcgacg
    19921 atatcgcgca gctcctgaat ctgatcttca ttaagctgct gcgcctgggc aagcgacggg
    19981 atcatcagcc gtgcattttc ggcatcctgc cgcgccacac tggcaaacgc caccgccgcc
    20041 atttgacggg taaaatcggt cgcgccggtt gtacgcgcga aggtcagtac cgtattaggg
    20101 ttgttcgcca gtgaaatgat tgccgaggcg atagtctggt aatcggcagg catctgccct
    20161 gccagcactg ttaccaggcc tgtgttaccc gctttcatcg ccagacggat acgctctaaa
    20221 tacgccagcg gatcttgttt acctgacgca cgccagacgc taaataactt gtcacaggcg
    20281 ttaggctggc tcttgccggt tagccacagc tctttcgccc cttgccaggc ttcttcactc
    20341 tgcccggtgt tccatttcgc atagtagtaa ttacattgcg cttcggtagt tccgggcttt
    20401 tccgggctaa aggctaacaa gccacgccag tcttcacgcc gcgccagttc attgacgaaa
    20461 cgagattgca gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa gttagtgacc
    20521 gtcaccgccg gttgattcat caggtcatcg gtgatctggc ggtattccag gtagggataa
    20581 agcggataat ccttcagtcc aggcatcatt tgttcgacca catccatctg tcgattatcc
    20641 caggcctgct tgatttgcgc gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc
    20701 acgctgctga ccgtcagcag acagacaccg gcagccaaca gccgccaggt aacttgtttg
    20761 gctttttcca caagcgcatc ctctaagtgt aaatacatca atgcagcgtc atgccgcgta
    20821 atagatttat gctaaccagt cattgccgtt tacgccacgt tacggacact tttttacttt
    20881 tactgcgaag gtgatcgacc aactgggatt aggcggggaa acaggctaca cttcgccttt
    20941 gaaaactcat catcacgata aacatagagg cgaagtccaa cgtggctcaa ttcgtttata
    21001 ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa aacatctctc
    21061 tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg ggtaagtcca
    21121 ccctgctgcg cattatggcg ggcattgata aagacatcga aggtgaagcg cgtccgcagc
    21181 cagacatcaa gattggttat ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc
    21241 gtgagtccat tgaagaagcg gtttcagaag tggttaacgc cctgaaacgc ctggatgaag
    21301 tgtatgcgct gtacgccgat ccggatgccg attttgacaa gctggccgct gaacaaggcc
    21361 gtctggaaga gatcattcag gctcacgacg gtcataacct gaacgtacag ctggagcgtg
    21421 cggcggatgc gctgcgtctg ccagattggg acgcgaaaat cgctaacctc tccggtggtg
    21481 agcgtcgtcg cgtggcgttg tgccgcctgc tgctggaaaa accagacatg ctgctgctcg
    21541 acgaaccgac caaccacctg gatgccgaat ccgtggcgtg gctggaacgc ttcctgcacg
    21601 acttcgaagg caccgtggtg gcgattactc acgaccgtta cttcctcgat aacgttgcag
    21661 gctggatcct cgaactggac cgcggtgaag gtattccgtg ggaaggtaac tactcctcct
    21721 ggctggagca gaaagatcag cgtctggcgc aggaagcttc acaagaagcg gcgcgtcgta
    21781 agtcgatcga gaaagagctg gagtgggtac gtcagggaac taaaggccgc cagtcgaaag
    21841 gtaaagcacg tctggcacgc tttgaagagc tgaacagcac cgaataccag aaacgtaacg
    21901 aaaccaacga actgtttatt ccacctggac cgcgtctggg cgataaagtg ctggaagtca
    21961 gcaacctgcg taaatcctac ggcgatcgcc tgctgattga tgacctgagc ttctcgatcc
    22021 cgaaaggggc gatcgtcggg atcatcggtc cgaacggcgc gggtaaatcg accctgttcc
    22081 gtatgatctc cggtcaggaa cagccggaca gcggcaccat cactttgggt gaaacggtga
    22141 aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc gtttgggaag
    22201 aagtttccgg cgggctggat attatgaaga tcggcaacac cgagatgcca agccgcgcct
    22261 acgttggccg ctttaacttt aaaggggttg atcagggtaa acgtgttggc gaactttccg
    22321 gtggtgagcg cggtcgtctg catctggcga agctgctgca ggttggcggc aacatgctgc
    22381 tgctcgacga accaaccaac gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc
    22441 tgctggagtt cccgggctgt gcgatggtta tctcgcacga ccgttggttc ctcgaccgta
    22501 tcgccacgca catcctggac taccaggatg aaggtaaagt tgagttcttc gaaggtaact
    22561 ttactgagta cgaagagtac aaaaaacgca cgctgggcgc agacgcgctg gagccgaagc
    22621 gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg cgcgaacgcc
    22681 ttatccggcc tacaaaacca gcaatttcaa tacgttgtaa aactgtaggc ctgataagac
    22741 gcggtaagcg tcgcatcagg cattgagcac cgaatgccgg atgcggcgtg aacacctttt
    22801 ccggtctaca acagccttag catcctcaat ttcgtggcgg gtcacccgtg caggtttttt
    22861 caaaccttgt ttaatctcga cagcctcttc cagagaggtt ttcaactcat caaaaaaact
    22921 cattttatac ctcgcctttg agcagtttcg tcacggttat ccttgctccc ccatcatctc
    22981 ccgcaccagc tccacgcagc gcaggaaacg gctgtcgtaa tcgtcctctt caacccgcac
    23041 gaattcgata ttgttctctt ccagcatctc caccagcaag ttctggaact ctttgcgatc
    23101 caccgaactg ccgaggctgc gtaaaccatc cgccacccac ggcgtgttgt tctccagcag
    23161 gatcaccaga tcgaaacggt attcatcaat cagcgcctgc acgaacggat gctcacgccc
    23221 ttcgtacttt ttgcagaacg cctgggtagt gacaaaatcg gtgtcaataa acgccacttt
    23281 attggcgtat ttcactgcaa aatcaatgta ttgtgcgtgg cccaacgcga ttttatcgta
    23341 gtcggaatac tgcaatgcga tctcatcacc gccgaggtgt gaaaagacat aatcgcggcc
    23401 atattcccac gcactggtgg tgttgaagat attggcaagt ttgtttacca gggtggattt
    23461 accgctcgac tcgccgccaa ggatcgccac ggtacgcaca aaaaacggct tcacttcggt
    23521 aggaatatat tcccagtagc ggaacgggtt ttcgcggatc tgcgcaccgc tgatactcat
    23581 aaaggtacgt ttcggatcga ccagcaccgt ctcgatcccc agatgttcca tatactgtgg
    23641 cgcatcggct tcttccgagg tgtagatcag gtccggctgg atcccctttt cagccataaa
    23701 ctttttgatg ccgttgctcc acacatccca gccatgcgga tacggctcca tgccctcttc
    23761 gttgaaagca tgaatacgga tatttttctg atatttaaag gtttgcaata gccagcgcag
    23821 acgatccggc accgtgggct gctgcgacat ggcgctgtct tcaaacaacg cgcggtcgcg
    23881 ggtatcgtca aaacccataa tgatatgcag ctcgtcaacc tggctacagg cgcgctggat
    23941 aaggtagata tgtccggtat gcagcgggta gaacttaccg aatacgacac caatggtttt
    24001 cttctgccgg ggaaattcaa gccccaaaaa acggtgcaat gcctccagct tttgcgcgct
    24061 ggggctttta attttggcat tcagtaactg gcttaaatac cctttggtca taccgctggc
    24121 atcagctacc tgctgtagcg tgcagccctg ttgcttgatg gcagttttca ggtaatcaaa
    24181 tgacgacata tctccctccg tatctctcat tataagtcgt cgaacacgct aagcgcgtcg
    24241 gagagttttt taacgccaaa aatctgcatc ccttccggcg cttttttcgg tacgttagcc
    24301 gccggaacaa tcgcccggcg aaaaccgtgt ttcgccgctt cagaaatccg ctcctggcca
    24361 ctgggcaccg ggcggatctc ccctgccagc ccgacttcac caaacaccac cagatcctgt
    24421 ggcagcggtc tgtcacgcag gctggaaacc atcgccagca gtaacgctaa gtcgccgctg
    24481 gtttcggtta ccttcacgcc gccgaccacg ttcacaaaca catcctgatc ggccatttgc
    24541 agaccaccgt gacggtgcaa gacagccagc aggattgcca gacggttttg ttccagcccc
    24601 actgccacgc ggcgcggatt cgccattatc gagtgatcga ccagcgcctg aatctccacc
    24661 agcaacggac gcgttccttc ccacaccacc atcacggagc tgccggaggt cacttcatct
    24721 ccgcgactta agaaaattgc cgaagggttg ctgacttcac gcagcccctg ttcggtcatc
    24781 gcgaagacgc ccagctcatt caccgcgccg aagcggtttt tatggctgcg taaggtgcga
    24841 aaacgggagt cggcatcgcc atccagaagc accgaacagt cgatacagtg ttccagcact
    24901 ttcgggccag ccagcgaacc atctttggtt acgtgtccaa ccatgacaat cgccacaccg
    24961 cgcgttttgg cgaaacgcgt cagataagcc gccgtttcac gcacctgcgc cacgctacca
    25021 ggcgatgatt gtacatccgc catatgcatc acctggatcg agtcaattac catcagcttc
    25081 ggttgctctt cttcggcaat caggcagatc tgctcgatgc tggtttccga caacatattg
    25141 agattgtcag tcggcaggcc aaggcgatga gcgcgcattg ccacctgttg cagcgactct
    25201 tcgccggtga catacagcgt tttcatctgc tgggccagtt tgcacagcgt ttgcagcaac
    25261 agcgtggatt tccccgcacc agggttaccg ccaatcagaa tggcacttcc tggcaccacg
    25321 ccgcctccta gtacgcggtc gaactcttta aatccggtgg aaaaacgcgg cagctcttca
    25381 aggctgatat cggagagttt ctggactttt gccaccccgg cgctaccggc atagccgctg
    25441 agacgctcgt tacgcgccac cgttggcgac gcagcaagac gcacctcggt gatggtgttc
    25501 caggcatgac aggcactgca ctgcccctgc cagcgcggat aatcggcccc gcattcatta
    25561 caaacaaagg cgcgttttgg agcttttgcc acggtgtacc tcgttaatgc tgtgccgccc
    25621 gcaggatgac gggcaggcaa ttacttctga ttcaggctgc cagagaggat gcagaatacc
    25681 cccatcaggt cagcgtgacg gatggtgact tccgcctttt cattcacttt tggcttggca
    25741 tggtaggcaa tccccagccc tgctgctttg atcatcggca ggtcattagc tccatcgcca
    25801 atcgccacgg tctgcgccag cgggatttca tactcctgcg cgaggcgagt cagagttttc
    25861 gctttgtact gcgcgtctac gatgtcgccg atcacattgc cggtaaattt accgtccatg
    25921 atctccagtt cattggctac cacggctgtc aggcgcagct tgtcgcgcag gtattcagca
    25981 aagaaagtaa agccgccgga ggcaatcgcc actttccagc ccagcgtttc cagcttgagc
    26041 accagctgcg ttaagcctgg catcagcggc agattttcac gcacctgttg cagaatattg
    26101 gcgtcagcgc ctttcagcgt cgccacgcgg ctgcgcaggc tggcggtaaa atcgagttcg
    26161 ccgcgcatcg cccgttcggt tacctccgcc accatctcgc ccgttccggc cagcttggca
    26221 atttcatcaa tacattcaat ctggatggcg gtggaatcca tgtccatcac cagcaaaccc
    26281 ggcgtgcgca ggtgcgggat tttccccagc ggggcgacat ccagctgcgc ttcgtgggcc
    26341 aggcgtgtag cccgtgcggt gagtgaacct gccagacgaa tcacctgata atcttccacg
    26401 caccaggcgg caacaatcac catcgccgca cccagtttgc tctggtattg ggtcagacgc
    26461 tgtttatcca gcccacgacc atacagcagc cagccgctac gacctgcgtg gtaatccagt
    26521 ggcatcactt catcaccact taatgaaaga ggcagacccg gccataaaga gacatcttca
    26581 ggcaggtcgc accaggtaat gttaggcatt aaggctcctg taaaatcgtt cgaagcaggg
    26641 aaaataacgc atgaggctac cttgtatcca ttgcttctgg caacattaag tctcaaattt
    26701 tcaaagggtg gaagatggct cgcacaaaac tgaaattccg gctacatcgg gcagtgattg
    26761 tcctgttctg tcttgccttg ttagtggcac tgatgcaggg ggcgtcatgg tttagtcaaa
    26821 accaccagcg acagcgtaat ccacagctgg aagaactggc ccgcaccctg gcgcgtcagg
    26881 tgacgctgaa cgttgcaccg ctgatgcgta ccgactcacc ggatgaaaaa cgcattcagg
    26941 cgatcctcga tcagttaaca gatgaaagcc gtatcctcga cgcgggtgtg tatgacgaac
    27001 aaggcgatct tatcgcacgt tctggcgaaa gcgtcgaagt gcgcgatcgg ctggcgctcg
    27061 acggtaaaaa agcaggcggc tattttaacc agcagattgt cgagccaatt gcgggtaaaa
    27121 acggaccgct cggctatctg cgcctgacac tcgacaccca tacgctcgcc accgaagccc
    27181 aacaggtgga taacaccact aacattttac gcctgatgtt gctgctctca ctggcaatcg
    27241 gcgtagtgct gacccgcacg ctgctacagg gtaaacgcac ccgctggcag caatcgccct
    27301 tcctgttaac cgccagcaaa ccggtgccgg aagaggaaga aagcgagaaa aaagagtgac
    27361 ccattactac aagaaaggaa atcgttatgt ccacattacg actgctcatc tctgactctt
    27421 acgacccgtg gtttaacctg gcggtggaag agtgtatttt tcgccaaatg cccgccacgc
    27481 agcgcgttct gtttctctgg cgcaatgccg acacggtagt aattggtcgc gcgcagaacc
    27541 cgtggaaaga gtgtaatacc cggcggatgg aagaagataa cgtccgcctg gcgcggcgca
    27601 gtggcggtgg cggtggcggt ggcggcgcgg tgttccacga tctcggcaat acctgcttta
    27661 cctttatggc tggcaagccg gagtacgata aaactctctc cacgtcgatt gtgctcaatg
    27721 cgctgaacgc gctcggcgtc agcgccgaag cgtccggacg taacgatctg gtggtgaaaa
    27781 ccgtcgaagg cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgcggct
    27841 tccaccacgg caccttgcta ctcaatgccg acctcagccg cctggcaaac tatctcaatc
    27901 cggataaaaa gaaactggcg gcgaaaggca ttacttcagt gcgttcccgc gtgaccaacc
    27961 tcaccgagct gctgccgggg ctcaccatga gcaggtttgc gaggccataa ccgaggcctt
    28021 tttcgcccat tatggcgagc acgtggaagc ggaaatcatc tccccggaca aaacgccaga
    28081 cttgccaaac ttcgccgaaa cctttgcccg tcagagtagc tgagagtgga acttcggtca
    28141 ggctccggca ttctcgcatc tgctggatga acgctttagc tggggcggcg tggaactgca
    28201 tttcgacgtt gaaaaaggcc atatcacccg cgcccaggta tttaccgaca gcctcaacct
    28261 cgcgccgctg gaagccctcg ccgggcgact gcaaggctgc ctgtaccgcg cggatatgct
    28321 gcaacaagag tgcgaagcgc tgttggttga cttcccggac caggaaaaag agctacggga
    28381 gttgtcgacg tggatagcgg gggcggtaag gtaactcgct gattaaaata tccaaggaag
    28441 gaaatatgat cgatctggaa aaccaggaac gggagattat caatttaatg ttcagccagc
    28501 gtatttcctg gctagccgcc gtgcgtatca ggcataaatt atcgcttgct gaagtaagta
    28561 aaatgttagg tatctcgatt aattctctaa aacagatcga aaagacggaa cggcttagct
    28621 caaacatcaa aagcaaaatg gcagaaattt atggttgtcc accagagttg ctgatatgcc
    28681 cttcctggat gacggctgaa cataaatgaa tagattcgtg aatagatact tgaatgtttt
    28741 ttattttcta tccatatgaa aaataatagt aaatctataa atcagttgct attgatgaat
    28801 agatactaaa aagccggagc agtcccccac tccggcttaa cacgacaatc gcctttcagc
    28861 gaaacacaat tactctttat cgcccagcag aacggattcc agtgcgattt tgatcatgtc
    28921 gttgaaggtg gtctgacgct cagcggcagt ggtctgctcg tgagtgcgga tgtggtcaga
    28981 cacggtgcag atggtcaggg ctttcgcgcc aaactctgca gccacgccgt agataccagc
    29041 cgcttccatt tccacgccga ggatgccgta tttttccatc acgtcgaaca tttcgccgtc
    29101 cggagagtag aacaggtcag cggagaacag gttacccacg cgagcatcaa cgcccagtgc
    29161 tttagctgca tctactgcgt tacgcaccat gtcaaagtca gcgatagcgg caaagtcatg
    29221 gtctttaaaa cggatgcggt taactttgga atcggtgcag gcccccatac cgataacgac
    29281 gtcgcgcagt tttacgtgcg gcagaactgc gccacaggaa ccaacgcgga taattttctt
    29341 cacgccgaaa tcggtgatca gttctttggt gtagatggag caggacggga tacccatacc
    29401 gtgacccatt acggaaattt tgcggccttt gtaagtacca gtgaagccca gcataccgcg
    29461 aacgttgttc acttcacggg catcttcaag gaaagtttca gcaatatact tcgcacgcag
    29521 cgggtcgcct ggcatcaaaa ctacgtcagc gaaatcgccc atttctgcat taatgtgtgg
    29581 ggtagccatt gttttatcct ttttgtgaca taacaaaggc ggagtcaacg ctccgcccaa
    29641 atccatcaga acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga
    29701 gtctggccga tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg
    29761 ccatatacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatcg
    29821 caaccgtggt cagcggtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc
    29881 ggcagacggc ggtcgaacag ttccagaccc gcagcataac cggcgacgtc gcggcggtgg
    29941 ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct
    30001 ttcatctctt tgatggtggc gtcaaacagc gcgtccaagc cagtcgcttt cacttttttg
    30061 gtgataccgc agttggcgta gatgtctgca attttaccga cagaaaccac ctggccgtgt
    30121 ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caagtcgtga
    30181 cggttaccgg tacgctggaa gttaccagct ttgtcaccga taaacggacg agcgataaca
    30241 cgaccgatat tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag
    30301 agtttatcca gaccgaaagt ttcttcatgg caggcaatct ggaacacgga gtcagcggag
    30361 gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagttgatc cagaatgacc
    30421 gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc gaccagttta
    30481 tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaacaga
    30541 accgggacac cggcgatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg
    30601 tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa
    30661 ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc
    30721 ggccctttac gaccattatc agcttcgcct ttggcacaag cttctgcgat atgacccagg
    30781 gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag
    30841 tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa
    30901 atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc
    30961 ggtgcgttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg
    31021 ttttcgtctt tcgcgaggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaga
    31081 cgcgccatat cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg acgacgaccg
    31141 ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct
    31201 tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc
    31261 tcaacgaagt cggtcgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct
    31321 ttaccgttgt ccagcaccgc ctgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt
    31381 ttgccggaga tcagcatctc cacgcacagc gccatcgtga catcaaacag acgcgggtta
    31441 cgatactcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg
    31501 gaggccagta cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta
    31561 gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg
    31621 ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgccag tttcttcgcc
    31681 aggatcgagg cagtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg
    31741 tagaaacgtt tatcagccgg agccagtgaa ctggtctgac cgataatcgc cacgccgacg
    31801 tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat
    31861 tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccggagat catcggaata
    31921 tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtgacatc gccgacgccg
    31981 ccggtcgagt gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg
    32041 gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg
    32101 aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg aaatagtgtt gtcgcgaata
    32161 ccgttgataa agaaacgaat ttcttcatcg ctcagcgcat gaccatcacg ttttttacga
    32221 ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt
    32281 gccggatgcg tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag
    32341 cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcgccg
    32401 tggcccagcg ctttcagcag gcttgccagc aggctggaag caccaaagcg gtagtgacgc
    32461 gcatcagccc agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct
    32521 tccgcagtac gcacgccgcc cgccggtttg aaaccaacgg ttttttctac gcccatatca
    32581 cggatcactt ccatcatgat gcgagcgctt tccggcgtcg cgttcacagc cactttaccg
    32641 gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc
    32701 agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc
    32761 gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtc acccgccatc
    32821 agcgcgcggt acgggaacac cacgtcaact tcatcagcac cgtaggcgat tgccgcacgg
    32881 gtttctgcca gcgcgatttc gatgtcgtcg ttaccgtgtg ggaagttggt taccgtagcg
    32941 atacggattt ccggggtgcc ctgctctttc agagttttgc gagcaatcgg gataaaacga
    33001 ggatagatac agatagcggc ggtattgccg accggagttt tggcctgatg acacagagca
    33061 attactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca
    33121 cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt
    33181 caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg atacacatca
    33241 caattaagga aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt
    33301 cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga
    33361 aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca
    33421 tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga
    33481 gcagaagcgc cattttctgg cactggaagc agaaaacaat ctgccttacc cacagcttcc
    33541 tgccgaagcc cgccgcgcgc tggatgaagg tgtaatctgc gatatgtttg aaggtcatgc
    33601 gccgtacaaa ccgcgctatg tcttacccga ttacgctcgt tttctggcga acggttccga
    33661 atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct
    33721 ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt
    33781 gttgcaaccg tatgttggaa ttctaacaca aaacgagatc gatgttcgaa taaaacgttt
    33841 ctggcgttac ctcgacagaa ccctgccaga tgcctttatg cacgccaata tcggcccgtc
    33901 tgattcgccc attacccgcg cgatcttacg tgcagacgcg caggtttcac cgaacctgac
    33961 ctttatctac gatcctgaaa tcacccctga tgacctgctg ctggaagtgg cgaagaacat
    34021 ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa ttttcacaaa
    34081 agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg gtggcagcac
    34141 gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg atgacttctt
    34201 tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg cgcggtgtga
    34261 attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag aagggctgat
    34321 taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag cggttaactt
    34381 gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg caaatgaagt
    34441 gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg tgaaatatgg
    34501 ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata tcggcaccac
    34561 gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcacccatc tgcaaactgt
    34621 cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc tcgacgaaac
    34681 catcaaacgt aacccgcagg cactggtaca gctttgcctc ggtgccttta aagccggaat
    34741 gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg gttatatggt
    34801 gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca ccacctggct
    34861 gggcgaagaa gccgcacgca acactcgtat tctggaacgc cagccgcgcg tgataagcca
    34921 tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt gacgggccag
    34981 gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg ctgcaaaaac tgtcacaatc
    35041 cgtggacgat gggacgttgc aatgactgtg gggagtgcgt gccacagtgt ccgcatcagg
    35101 cgttgcagat tgttgacggc aaagtggtgt ggaacgctgt ggtctgcgag caatgtgata
    35161 cctgcctgaa gatgtgtccg caacatgcca caccaatggc gcaatccatg agcgtggacg
    35221 aagtgcttag ccatgtgcgc aaagcggtgc tgtttattga agggataacg gtaagcggcg
    35281 gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc aaaaacgatc
    35341 cgcaactgcg ccatctcacc tgtctggtgg acagtaacgg tatgttgagc gaaaccggct
    35401 gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagca tggggtagcg
    35461 aatgtcatca acaattaacc ggacgcgata atcaacagat taagcgcagc atctatttgc
    35521 tggcagagcg cggcaagctg gcggaactgc gtttgctgat gatcccaggc caggtggatt
    35581 atttgcaaca catcgaagaa ctggcggcgt ttatcaaggg acttggtgat gttccggttc
    35641 gcctgaacgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg gcgagcgcca
    35701 cgccggaaga cgttgagccg ttggctgatg cattaaaggt gcgcggggtg agccggttga
    35761 tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc acagacttgt
    35821 aggcctgata agacgcgtca agcgtcgcat caggcattgt gcgccaactg ccggatgcgg
    35881 cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat aagacgcgtt
    35941 aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag ggcaaactac
    36001 ccacgaacgt taaacaccgc atacgtgtta ttaagcaaca cttcggcaat ctcatccgcg
    36061 ggttccgggc gtaactcgca aagcacggca aacacacggg cagcctgttc cgggcggtta
    36121 ggctgcccct gaaaaccgtt gagcggcata tccggcgcat cggtttccag caacaacgat
    36181 gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata ggtgatagta
    36241 ccgcctacgc cgattttgta gcccagctgt acaaaccgtt cagcctgttg ctggctgcca
    36301 gaaaatccgt ggacaacacc agcacgcggc aaatcgtggt gtttaagatg catcgccagt
    36361 ttgtcgtgcg tgcgccgtga atgcaggatc accggcagat cgtagcgttt cgccagtttc
    36421 agttgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc gaagagatcc
    36481 agaccgatct cccccaccgc caccaccttc gccggacgcc tttccagcgc ctgctgtagc
    36541 tgctcaagag acacatcgct atgtttttcc aacataccag gatgcaagcc caatgcggca
    36601 tacagcggtt gataattttc cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc
    36661 gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca
    36721 tcgccaataa aaggcgggaa atcaaaatgg cagtgagtgt cgataaaacg gcaaatcaag
    36781 ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcgctc acttcggcaa
    36841 ccagcgtatc gttggcgact ggcgcaggcg gaatgacaat cgattccggc gtcaccactg
    36901 gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc gtggcgagga
    36961 aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga atccgactgc
    37021 caagcgcgat actatgtaat ggcttcggcg gataaatttc ggatatccgc agcttgcccg
    37081 gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc tgcaccagat
    37141 tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg aaccactgcg
    37201 gcgtgtagta catttgtgac ggcacagtgc ggatgacgac caacgtttta gcgccctgcc
    37261 ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag ttaatgcctt
    37321 ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga atcacatcca
    37381 gccagttttg tttggttggt aaaaagtaat tcggctcgta gtcatcctga cgacaggcgc
    37441 acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt tgcaacggca
    37501 tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct ccacggacaa
    37561 agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact ttgcgcgcgt
    37621 aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat gtgccgagat
    37681 aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa
    37741 ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg aacggcctta
    37801 gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccact tctgccttcc
    37861 tgtttttgtt gtgaaagaaa gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa
    37921 gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt
    37981 tttcggccca tgaacaaact cactaggaac agaataatcc cgacgacaaa gacaatttta
    38041 gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc gattaacgcg
    38101 ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat agttaatgtc
    38161 accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg tgcacatcgc
    38221 gcctcccgac aaagttcggg aggacgaatt acttagtttt cagatcattt ttaacgcttt
    38281 tcacaccatc taccgctttg gcgatacttt cagcacggtc actttgtgcc tgagaatcta
    38341 cggtaccgga gagctgaacc acgccatcgg tggtttcaac tttcacttta cgtgaaggga
    38401 cgatatcgtc cgccagcagt ttggctttga tttcactggt ggtggcggtg tcacccgcgt
    38461 agcccttcac cgagccttct ttagcgtcgc gaacgtgcag tttgtcgctg acagaggtca
    38521 cgccttcaac gcctttcgcc actttcactg cctcttcggc ctgggcctga ctttcaacga
    38581 aaccgctcag ggtcacgact ttttgatcgg ttttgacaga gatatcggtg ctcttgatgt
    38641 tgtcatgatc caccagcgcc gccttcactt tcgcggtgat ggcgctgtca tccatgaaat
    38701 taccgacttt attcatagag ctatcgactt tttgccctgc gctttcattg gtagtctgcg
    38761 cgttgttttc cgcgtaggca gagccggtcg cgacggcaga ggtcaacatt acagccagca
    38821 gagttttcga aatcttcagt cttgtcatag tcatcgattt attcctgtat gtttgctcgt
    38881 aatttgagcc tagcaacacg aggttgcatt gctgaatagg gagagacttc accctctaca
    38941 gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa taacgaccat tacagcctct
    39001 gaatcagtta ttaatatcgg tagaatgaca atcgacggct ttagatactg atatctacgc
    39061 attgaacggt atttaacgcc atcagaaatg tcatcacttt gttaaatata gatcacaatt
    39121 ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag tgaatgtttt aagaacattc
    39181 cgtaagcggc taataaggaa gggaaattga cagggcgcag cggctaccgc gccctggagg
    39241 caagaattaa tgctcgcggg tctggtggaa ctgaacgtcc ggataacgtt cctgtgccag
    39301 gcgcaggttg accatgctgg tagcgatgta agcgaggtta tcgccgccat caagcgccag
    39361 ttggctttcg ttcttacgct tgaactcttc gaatttcttc gcgtctgcac attctaccca
    39421 gcgggcagtg gcaacgttga ctgactcata cactgcttca acgttgtatt cgctcttcag
    39481 gcgcgctacc accacatcaa actgcagcac accaaccgca ccaacgatca gatcgttgtt
    39541 ggagattgga cggaacacct gcaccgcgcc ctcttcggaa agctgtacca gccctttgag
    39601 cagctgtttt tgcttcagcg gatctttcag gcggatacga cggaacagtt ctggtgcgaa
    39661 gttcggaata ccggtgaact tcatcatctc accctgggta aaggtgtcgc cgatctgaat
    39721 ggtgccgtgg ttgtgcaggc cgaggatatc gcccggatac gcttcttcaa cgtgcgaacg
    39781 gtcacccgcc ataaaggtca gcgcgtcgga gatcaccaca tctttcgcag tgcgcacctg
    39841 gcgcagtttc atgccttttt catatttacc ggacaccaca cgcataaacg ccacgcggtc
    39901 gcggtgtttc ggatccatgt tggcctgaat tttaaatacg aagccggtaa atttatcttc
    39961 gctcgcttct acggtacggg tatcggtctg acgcggcatc ggcgcaggcg cccactccac
    40021 caggccattc agcatatgat caacgccgaa gttacccagc gcagtaccga agaataccgg
    40081 agtgatttcg cccgcaagga acagctcttt gtcgaactcg ttagacgcgc ctttaaccag
    40141 ttccagttcg tcacgcagct gctgtgccag atcttcacca accgcagcat cgagatccgg
    40201 gttattcagc cctttaacaa tgcggacttc ctgaatggtg tgccctttac cgctctgata
    40261 gagataggtt tcatctttat aaaggtggta aacgccttta aacagcttgc cgcagccaat
    40321 tggccaagtg atcggtgcgc agccaatttt cagctcgttc tcaacttcat cgagcagttc
    40381 catcgggtcg cggatatcac ggtcaagttt gttcataaag gtgaggatcg gcgtgtcgcg
    40441 cagacgggta acttccatca gcttacgggt acgatcttca acaccttttg cggcgtcgat
    40501 aaccatcagg cagcagtcca ccgccgtcag ggtacgatag gtatcttccg agaagtcttc
    40561 gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga tacggaaact gcatcacaga
    40621 cgtagtaatg gagatcccac gctgcttttc catctccatc cagtccgact tagcgtgctg
    40681 gttggaacca cggcctttta ctgtaccggc ggtctgaatg gcctgtccga acagcagcac
    40741 cttctcggtg atggtagttt taccggcgtc cgggtgagaa ataatggcaa aagtgcggcg
    40801 cttcgccacc tctttcaaat aaggagacaa cgtcataatt ctttcttctt gagtaagcgg
    40861 catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat tttacccatc aacggggggg
    40921 aggcaatcag tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt
    40981 gggcgcgatg ccttctggtt gctcgcgatt gtgcgcattc agccagcagg tcgcaagccc
    41041 ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg ccaaccatca gcacgcgtga
    41101 acggtcagga ttgcccgcct gttccagcgc ataatcgaaa attttcttat tcggtttggc
    41161 aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa tcacgcaggc ccgtccgttc
    41221 cagacgcact tgctgcaagg cgctaaaacc gttggtgatg atgccgattt tagcgttgct
    41281 acgaatggcg ttaagcagag aaaccgcgcc cggcaacggc gtacagattt ccgccatcgc
    41341 attaataaag gcatcgttaa gcaagcctgc ttcaactttc agccgttcgg cccagctctc
    41401 gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg ttttgataat ccacccacag
    41461 tggcttgtta acggcctgat agtcctgaaa atcttcagcg gtaaaggtga cgctgtaatc
    41521 aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta aacagcgttt catcggcatc
    41581 aaagaaaatc cagtcccact tcattattcc accttgtctt acatactgat tggcaacgcc
    41641 atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg aatcgtcgcc
    41701 tcgttaaagc ctaaactttc gtacagggca atggcggcag catttgaggc acggacttcc
    41761 agccatagtg tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc
    41821 ctacccagcc cctgacgctg atagtcggga tcgaccgcaa tattgaacaa tgtagcttca
    41881 tccagcacca cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt taactgaaag
    41941 ttgagataac gctcgccctg gttgctggca aaggtttttt cactccacgg aaaggagtgg
    42001 gcgcgttgtt caatgtggta agccgctggt aaatcagtcg tttcgaggga agaaatcgtg
    42061 ttcatatgtg caaatttgtt gccataacgc ggcgcgtgcc gttgggtttg cccgtaattc
    42121 ggtgagcgcc ggtgatgcca cctgagcgcc ttccaatgat agcggttcgt cagtacccaa
    42181 ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg attttttctg gcgtcagttg
    42241 cagcacttga tcggggctga cggttaatgc gcgcagaaca tcgctcacta aaggatcagt
    42301 cagggcggga agatcgtttg ccaccatcac cagacggacg tgtgccgggc tcgcaatggc
    42361 aatctccccc tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc ccagttgctg
    42421 taactgccag tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata
    42481 tagcaaattc gtcgataccg cgccaacata tggctataat cgccgccagt atcaattgag
    42541 gagcattcca tgtctgcatt taccccggca agtgaagtct tgctgcgtca cagtgatgat
    42601 ttcgaacaaa gccgtattct gtttgccgga gacttacagg atgacctgcc cgcgcgttta
    42661 gataccgcgg ccagccgtgc tcatacccag caattccacc attggcaggt gttaagctgc
    42721 cagatggggg ataacgcccg ttttagtctg gtcgccacgg cgaatgatgt cgcagattgc
    42781 gatacgctga tttactactg gccgaagaac aaaccggaag cccagttcca gttgatgaat
    42841 ttactttctc tgctgccagt ggggacagat atttttgtcg ttggcgagaa ccgcagcggc
    42901 gtgcgcagcg ctgagcagat gctggcagat tatgcgccgt tgaataaagt cgacagcgct
    42961 cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc cagtatttga tgcggataaa
    43021 ttctggggcg aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg cgtgtttagc
    43081 cgcgacggtc tggatgtcgg tagccagttg ctgctctcga cgttaactcc gcacacgaaa
    43141 ggtaaagtgc tggatgtcgg ctgtggcgcg ggtgtacttt cagttgcctt tgcgcgccat
    43201 tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgccc cggcggtaga agccagccgc
    43261 gcaacacttg cagccaacgg tgttgaaggt gaagtctttg ccagcaacgt cttttccgag
    43321 gtgaaaggtt gttttgatat gatcatctcc aacccgccgt tccacgatgg gatgcaaacc
    43381 agcctggatg cggcgcaaac gctgattcgt ggcgcggtgc gtcatcttaa tagcggcggc
    43441 gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg acgtgctgga tgagacattt
    43501 ggcttccatg aagttatcgc gcaaaccggg cgcttcaagg tatatcgcgc cattatgacc
    43561 cgccaggcga agaaaggttg agtatctgat atctgtcgcc gcataccgtg tcggatgcgg
    43621 cgtgaacgcc ttatccgacc tacaaaacct gcacgttagc ccttcgtagg ccagataaga
    43681 cgcgccagcg tcgcatctgg catctgcccc cgcatgccta ccaaacctgc acgctagttt
    43741 cctgatggac atttttccag caattacaca tcaggcgata attaactatt gacgaaaagc
    43801 tgaaaaccac tagaatgcgc ctccgtggta gcaattcttt ttaagaattg atgg
//
LOCUS       DADLDT020000020        40802 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000020 DADLDT020000000
VERSION     DADLDT020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40802
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.020"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="JQH22_001041"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="JQH22_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFB2205441.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="JQH22_001042"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="JQH22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFB2205442.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="JQH22_001043"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="JQH22_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFB2205443.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="JQH22_001044"
     CDS             complement(3100..6843)
                     /locus_tag="JQH22_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFB2205444.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWDTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="JQH22_001045"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="JQH22_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFB2205445.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="JQH22_001046"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="JQH22_001046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFB2205446.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="JQH22_001047"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="JQH22_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFB2205447.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="JQH22_001048"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="JQH22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFB2205448.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="JQH22_001049"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="JQH22_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFB2205449.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="JQH22_001050"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="JQH22_001050"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFB2205450.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="JQH22_001051"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="JQH22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFB2205451.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="JQH22_001052"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="JQH22_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFB2205452.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="JQH22_001053"
     CDS             16240..16347
                     /locus_tag="JQH22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFB2205453.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="JQH22_001054"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="JQH22_001054"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFB2205454.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="JQH22_001055"
     CDS             complement(17386..18195)
                     /locus_tag="JQH22_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HFB2205455.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="JQH22_001056"
     CDS             complement(18199..18591)
                     /locus_tag="JQH22_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HFB2205456.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="JQH22_001057"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="JQH22_001057"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFB2205457.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="JQH22_001058"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="JQH22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFB2205458.1"
                     /translation="MKPSIVAKLEALHERHEEVQTLLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="JQH22_001059"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="JQH22_001059"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFB2205459.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="JQH22_001060"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="JQH22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HFB2205460.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="JQH22_001061"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="JQH22_001061"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFB2205461.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="JQH22_001062"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="JQH22_001062"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFB2205462.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="JQH22_001063"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="JQH22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFB2205463.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="JQH22_001064"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="JQH22_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFB2205464.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="JQH22_001065"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="JQH22_001065"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFB2205465.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="JQH22_001066"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="JQH22_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFB2205466.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="JQH22_001067"
     CDS             29637..32504
                     /locus_tag="JQH22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFB2205467.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="JQH22_001068"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="JQH22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HFB2205468.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="JQH22_001069"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="JQH22_001069"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFB2205469.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="JQH22_001070"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="JQH22_001070"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HFB2205470.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="JQH22_001071"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="JQH22_001071"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HFB2205471.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="JQH22_001072"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="JQH22_001072"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFB2205472.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="JQH22_001073"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="JQH22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10086 a        10575 c        10175 g         9966 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgtcccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgtctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcttggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 at
//
LOCUS       DADLDT020000021        40713 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000021 DADLDT020000000
VERSION     DADLDT020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40713
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.021"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            155..496
                     /locus_tag="JQH22_001074"
     CDS             155..496
                     /locus_tag="JQH22_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFB2205473.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(487..1413)
                     /locus_tag="JQH22_001075"
     CDS             complement(487..1413)
                     /locus_tag="JQH22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2205474.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1503..2501
                     /locus_tag="JQH22_001076"
     CDS             1503..2501
                     /locus_tag="JQH22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HFB2205475.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2498..2716)
                     /locus_tag="JQH22_001077"
     CDS             complement(2498..2716)
                     /locus_tag="JQH22_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFB2205476.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2718..4733)
                     /gene="ligA"
                     /locus_tag="JQH22_001078"
     CDS             complement(2718..4733)
                     /gene="ligA"
                     /locus_tag="JQH22_001078"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFB2205477.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4804..5790)
                     /gene="zipA"
                     /locus_tag="JQH22_001079"
     CDS             complement(4804..5790)
                     /gene="zipA"
                     /locus_tag="JQH22_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFB2205478.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6020..6781
                     /gene="cysZ"
                     /locus_tag="JQH22_001080"
     CDS             6020..6781
                     /gene="cysZ"
                     /locus_tag="JQH22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFB2205479.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6966..7937
                     /gene="cysK"
                     /locus_tag="JQH22_001081"
     CDS             6966..7937
                     /gene="cysK"
                     /locus_tag="JQH22_001081"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFB2205480.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8321..8578
                     /gene="ptsH"
                     /locus_tag="JQH22_001082"
     CDS             8321..8578
                     /gene="ptsH"
                     /locus_tag="JQH22_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFB2205481.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8623..10350
                     /gene="ptsI"
                     /locus_tag="JQH22_001083"
     CDS             8623..10350
                     /gene="ptsI"
                     /locus_tag="JQH22_001083"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFB2205482.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10391..10900
                     /gene="crr"
                     /locus_tag="JQH22_001084"
     CDS             10391..10900
                     /gene="crr"
                     /locus_tag="JQH22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFB2205483.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10942..11793)
                     /gene="pdxK"
                     /locus_tag="JQH22_001085"
                     /gene_synonym="thiJ"
     CDS             complement(10942..11793)
                     /gene="pdxK"
                     /locus_tag="JQH22_001085"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFB2205484.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11898..12272
                     /locus_tag="JQH22_001086"
     CDS             11898..12272
                     /locus_tag="JQH22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFB2205485.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12305..13028
                     /locus_tag="JQH22_001087"
                     /pseudo
     CDS             12305..13028
                     /locus_tag="JQH22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13232..14143)
                     /gene="cysM"
                     /locus_tag="JQH22_001088"
     CDS             complement(13232..14143)
                     /gene="cysM"
                     /locus_tag="JQH22_001088"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFB2205486.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14277..15374)
                     /gene="cysA"
                     /locus_tag="JQH22_001089"
     CDS             complement(14277..15374)
                     /gene="cysA"
                     /locus_tag="JQH22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFB2205487.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15364..16239)
                     /gene="cysW"
                     /locus_tag="JQH22_001090"
     CDS             complement(15364..16239)
                     /gene="cysW"
                     /locus_tag="JQH22_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFB2205488.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16239..17072)
                     /gene="cysT"
                     /locus_tag="JQH22_001091"
     CDS             complement(16239..17072)
                     /gene="cysT"
                     /locus_tag="JQH22_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFB2205489.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17072..18088)
                     /gene="cysP"
                     /locus_tag="JQH22_001092"
     CDS             complement(17072..18088)
                     /gene="cysP"
                     /locus_tag="JQH22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFB2205490.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18392..19183)
                     /gene="ucpA"
                     /locus_tag="JQH22_001093"
     CDS             complement(18392..19183)
                     /gene="ucpA"
                     /locus_tag="JQH22_001093"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFB2205491.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19370..20170)
                     /gene="murR"
                     /locus_tag="JQH22_001094"
     CDS             complement(19370..20170)
                     /gene="murR"
                     /locus_tag="JQH22_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFB2205492.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20334..21230
                     /gene="murQ"
                     /locus_tag="JQH22_001095"
     CDS             20334..21230
                     /gene="murQ"
                     /locus_tag="JQH22_001095"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFB2205493.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21234..22658
                     /gene="murP"
                     /locus_tag="JQH22_001096"
     CDS             21234..22658
                     /gene="murP"
                     /locus_tag="JQH22_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFB2205494.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22838..23737)
                     /gene="yfeX"
                     /locus_tag="JQH22_001097"
     CDS             complement(22838..23737)
                     /gene="yfeX"
                     /locus_tag="JQH22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFB2205495.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23833..24408)
                     /locus_tag="JQH22_001098"
     CDS             complement(23833..24408)
                     /locus_tag="JQH22_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFB2205496.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24469..24918)
                     /locus_tag="JQH22_001099"
     CDS             complement(24469..24918)
                     /locus_tag="JQH22_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFB2205497.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24905..25330)
                     /locus_tag="JQH22_001100"
     CDS             complement(24905..25330)
                     /locus_tag="JQH22_001100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFB2205498.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25544..26413
                     /gene="amiA"
                     /locus_tag="JQH22_001101"
     CDS             25544..26413
                     /gene="amiA"
                     /locus_tag="JQH22_001101"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFB2205499.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26417..27316
                     /gene="hemF"
                     /locus_tag="JQH22_001102"
     CDS             26417..27316
                     /gene="hemF"
                     /locus_tag="JQH22_001102"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFB2205500.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27322..28374)
                     /gene="eutR"
                     /locus_tag="JQH22_001103"
                     /pseudo
     CDS             complement(27322..28374)
                     /gene="eutR"
                     /locus_tag="JQH22_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28420..28920)
                     /gene="eutK"
                     /locus_tag="JQH22_001104"
     CDS             complement(28420..28920)
                     /gene="eutK"
                     /locus_tag="JQH22_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFB2205501.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(28933..29592)
                     /gene="eutL"
                     /locus_tag="JQH22_001105"
     CDS             complement(28933..29592)
                     /gene="eutL"
                     /locus_tag="JQH22_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFB2205502.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30774..31565
                     /locus_tag="JQH22_001106"
     CDS             30774..31565
                     /locus_tag="JQH22_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205503.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32285..32680
                     /locus_tag="JQH22_001107"
     CDS             32285..32680
                     /locus_tag="JQH22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFB2205504.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32698..32961
                     /locus_tag="JQH22_001108"
     CDS             32698..32961
                     /locus_tag="JQH22_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205505.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            32984..33526
                     /locus_tag="JQH22_001109"
     CDS             32984..33526
                     /locus_tag="JQH22_001109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205506.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33665..33952
                     /locus_tag="JQH22_001110"
     CDS             33665..33952
                     /locus_tag="JQH22_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205507.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            33991..34197
                     /locus_tag="JQH22_001111"
     CDS             33991..34197
                     /locus_tag="JQH22_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205508.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34509..34994
                     /locus_tag="JQH22_001112"
     CDS             34509..34994
                     /locus_tag="JQH22_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFB2205509.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            34975..35253
                     /locus_tag="JQH22_001113"
     CDS             34975..35253
                     /locus_tag="JQH22_001113"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205510.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35246..36706
                     /locus_tag="JQH22_001114"
     CDS             35246..36706
                     /locus_tag="JQH22_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2205511.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36740..37549
                     /gene="murI"
                     /locus_tag="JQH22_001115"
     CDS             36740..37549
                     /gene="murI"
                     /locus_tag="JQH22_001115"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFB2205512.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37668..38362)
                     /locus_tag="JQH22_001116"
     CDS             complement(join(37668..38116,38116..38362))
                     /locus_tag="JQH22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFB2205513.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38881..40254)
                     /gene="eutA"
                     /locus_tag="JQH22_001117"
     CDS             complement(38881..40254)
                     /gene="eutA"
                     /locus_tag="JQH22_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFB2205514.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40229..40708)
                     /gene="eutP"
                     /locus_tag="JQH22_001118"
     CDS             complement(40229..40708)
                     /gene="eutP"
                     /locus_tag="JQH22_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFB2205515.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        10301 a        10481 c         9875 g        10056 t
ORIGIN      
        1 ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc ctaaaggcgc
       61 ttttttgcta tctgcgattt gtaagattga tttttcttct caagttcctt gttcttctac
      121 cgatattacc tcgtacagtg acgaggactg aacaatggcc actatctctt caacttcaat
      181 tccttccata caaacacaga gtagtaacag ggcatcacaa ggtagcgatg ttgcttcaca
      241 aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa caaattaaag agattgtaga
      301 cactagcggc agtgctgaag acaagcaaaa acaggccgaa ttgattcaac agcaaatcac
      361 cctactggaa acacaactgg cgcagttgca gaaacagcag gcagaaaagg cgcaggagaa
      421 agagcagcgt ttgtctctga atgtatccct tctaaatcct gttgaaaaaa cgactcatat
      481 cgatatctat atctgataca acggatcccc ttcccgacca gccattagcg cattgccctg
      541 gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt tcttcctgcg
      601 caccagcatc aactggcgtt caaccaccgg cgtaatgcgc ttaaccacca gtgggctacc
      661 ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg ccgatgcctg cggctaccat
      721 cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa taccattgcg
      781 cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca acaccaattt
      841 cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc tatcgcgatg
      901 gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc ccactgggcc
      961 tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca tcacccactg
     1021 ctgcggacga tcatgcagga caaactgaat atctggatag cggcgatggc tttcagcaat
     1081 gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa cttttccgct
     1141 cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat ccagcagtcg
     1201 ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac gcgtggttct
     1261 gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac ttactgccga
     1321 ctggctcaag ccgatacgct ctcctgcacg actaaaactt ttctcctgcg ctactgtgac
     1381 gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat ggatgcaata
     1441 aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca tctggagtct
     1501 ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg gtggtgttgc
     1561 tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat ctgaccaccg
     1621 cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag gcgattattg
     1681 ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc gtgctgtttc
     1741 cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg atgctctact
     1801 ccggttttct ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc gccttcacgt
     1861 caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc agcctgctgg
     1921 ggattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt gcaaggggca
     1981 gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg ttggggcatc
     2041 tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt gcgaaaactg
     2101 accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc gttaatggta
     2161 tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc tgcgttcttc
     2221 tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgagcttc aataaggcag
     2281 atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc atcccgatgg
     2341 caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg atgattttcc
     2401 atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc cagaccgaac
     2461 agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc ttcaggggct
     2521 ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt agctcaccga
     2581 gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc tccggcaggt
     2641 caattaagcg acgccctttg tatttgccaa acggcattat cgtattggct atttcaatca
     2701 gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc gtcgatgact
     2761 tcaatgccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc cgctatcacc
     2821 agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac cagtcgagct
     2881 ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt tttaccagca
     2941 aacgggctgt caatctcttc cgcgttgata acgatcggcg caggccagtg aacaccttcc
     3001 gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt gtgaacgtgg
     3061 gatgcaacga caatgccaac atcaggcacc ttttgcagct cttcaatcga agcggcttcc
     3121 agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc gccgacttca
     3181 cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt ttccagcgcg
     3241 ttaaccacgt tttgtgccaa ttttggcccc atacgctcca gtccggtcag tttgcctgcg
     3301 gtgagtttga acagatctgc cggagtatgg acatattctt tttcaaccag ctgatcgatg
     3361 attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg tttcagcgac
     3421 tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc ttcaccttcc
     3481 acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac ctcacgggta
     3541 tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc gccagcgcgg
     3601 cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc cgcattgtgt
     3661 aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg cgcaacaggc
     3721 gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt catctgctcc
     3781 tgcgccggga atttaaacgc aaccgcccag cgcggggcac gcgcgacaaa gccaagctgc
     3841 tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa gcccagcgtc
     3901 gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga ttcacaaagc
     3961 gttacccgat cgctgaccgg caccccccac tttttaaatt gcagtaaacg gccaagatga
     4021 gtatccggca gctcgccacc ttccagaaca ccaacgccat agcagaaaaa agtgagcggt
     4081 cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc attacgtggg
     4141 ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc gaaccccgcc
     4201 tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc tccgtgcagc
     4261 ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc ggtggtgcca
     4321 tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaagg
     4381 ccatccagct tcagctcaca gcaccaggtg actttctcgt tgtttttcag acggtcctgc
     4441 acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag tgacagcatt
     4501 ggtacttcat ggcgtatctg gctgaaagct gccagcggcg cagcacctac acgttgggta
     4561 ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag ttcgcgcatc
     4621 agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca
     4681 tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac
     4741 catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt tgccttaagt
     4801 gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg
     4861 cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat cggcaatatg
     4921 ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag acggtacctg
     4981 cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa aggttcccgg
     5041 tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc taagatgacg
     5101 atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt taagaagcag
     5161 ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca ccgcttcttt
     5221 gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct gtggtgccgc
     5281 tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat gcacaggctg
     5341 cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag gctgcggctg
     5401 ataggcaggc tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc
     5461 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gtaggtactg
     5521 gtgttgcggc gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac
     5581 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
     5641 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
     5701 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
     5761 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
     5821 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
     5881 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
     5941 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca
     6001 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
     6061 ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt
     6121 accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct
     6181 cgatgtctgg atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta
     6241 tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac
     6301 gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg
     6361 attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg
     6421 aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact
     6481 aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt
     6541 tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc
     6601 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
     6661 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt
     6721 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta
     6781 acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc
     6841 ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt
     6901 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
     6961 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
     7021 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
     7081 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
     7141 tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct
     7201 atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg
     7261 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag
     7321 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc
     7381 tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc
     7441 cggaaatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
     7501 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
     7561 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag
     7621 agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
     7681 tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
     7741 cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg
     7801 ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc
     7861 taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga
     7921 aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg
     7981 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
     8041 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
     8101 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gcttaatgac
     8161 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt
     8221 cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
     8281 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
     8341 cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
     8401 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
     8461 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
     8521 cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
     8581 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
     8641 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
     8701 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
     8761 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
     8821 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
     8881 caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa
     8941 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
     9001 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
     9061 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
     9121 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
     9181 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
     9241 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
     9301 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg
     9361 gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt
     9421 caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
     9481 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg
     9541 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg
     9601 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
     9661 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
     9721 aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg
     9781 cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
     9841 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
     9901 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
     9961 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
    10021 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    10081 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    10141 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    10201 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    10261 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    10321 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    10381 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga
    10441 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    10501 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg
    10561 taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc
    10621 gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt
    10681 tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg
    10741 cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac
    10801 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt
    10861 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    10921 ggcgcccatc ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct
    10981 ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc
    11041 atcgtttaac gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc
    11101 agtccctttc aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact
    11161 gaccaccacc acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac
    11221 ccattttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg
    11281 gcaattttta ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag
    11341 cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc
    11401 aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc
    11461 agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac
    11521 agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca
    11581 ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac
    11641 cgtcggcacg gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt
    11701 gcctacgctt ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct
    11761 actcttatcg ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc
    11821 tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac
    11881 aaagataagg taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac
    11941 tccccgctta cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg
    12001 gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg
    12061 aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg
    12121 agcagtttat tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga
    12181 agatccccgg taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga
    12241 cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg
    12301 taacatgaaa aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga
    12361 ttacgaaggg gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca
    12421 tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc
    12481 aaaaatgaaa aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt
    12541 tgatgatgaa gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt
    12601 tactgatcca ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg
    12661 tgctgatgct ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc
    12721 aaatgttaat tgaaactatg tggggtatga aatacatcgc tatggacagt attcttgaag
    12781 aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta
    12841 cctacgcgac tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa
    12901 agatgggact caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg
    12961 acggtcagga ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg
    13021 aaaattaaaa actgcatttg tcggcagcaa caactgccga caaatcagat tattgacaaa
    13081 gtgcactttg tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta
    13141 attcaatata ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc
    13201 gtttgtcatc agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct
    13261 cttccccaaa cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca
    13321 ccaccgcacc agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc
    13381 tgacgccaca gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc
    13441 gctgatgaat atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt
    13501 ccgcaggcca gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa
    13561 tggtcaccgg tttggattgt tcgcgcataa agcatgaaac gccggtgata gtgccggtcg
    13621 tccccatgct ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc
    13681 cggtggtggt gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc
    13741 cttcgccacg attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt
    13801 tggtgacaag aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca
    13861 tgttgtcggg catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg
    13921 caatgccggt gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc
    13981 gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt
    14041 tattgccttc cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact
    14101 tcaccagagg cgtattgcct attgtttgtt ctaatgtact cacaatctct atccatacgt
    14161 ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca
    14221 ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag
    14281 gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac
    14341 agccgcgcat gttgcagacc aacgaataaa cgctcgccat gctgcggggc atcgtcgcca
    14401 tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg
    14461 tagtgacctt tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta
    14521 cggcggctga tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca
    14581 ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt
    14641 ccctgcaggc ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc
    14701 catacctgat tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga
    14761 tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc
    14821 tcttcatgca gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg
    14881 ccgaacggtt catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc
    14941 acacgctgtt tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc
    15001 tggaccattt ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg
    15061 cgacgcggca gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac
    15121 agcgcgtaat gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg
    15181 tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc
    15241 agcagcgtgg ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga
    15301 atatccagtg agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca
    15361 atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca
    15421 ctgcaacata ctctttaaaa acagggtgat aatcgccatc aacgtcaaca gcgcggcggc
    15481 ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga
    15541 aagtgtttcg ccgcgaatcg agccggaaac caccgacact gcaccaaact cgccaatcgc
    15601 gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac
    15661 gcgacggaac atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct
    15721 gccctggctt aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat
    15781 ggtgaccagc accattccgg gccaggagaa cataatttgc agattatgtt catccagcca
    15841 gccgccgagc gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg
    15901 cgatacagca aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa
    15961 gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag
    16021 cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa
    16081 aaccggcatc agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag
    16141 gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg
    16201 gcgcgcgtca taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga
    16261 ccaaagcgac tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc
    16321 accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc
    16381 agcgacgtca cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat
    16441 tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc
    16501 ggcagcacca ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct
    16561 tcatattccg ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg
    16621 ctggtaaagg ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac
    16681 ttcgccagcc attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg
    16741 gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc
    16801 ggaaagcgat aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc
    16861 gatgccacaa acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg
    16921 atcacctccc agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc
    16981 ggcagcagca atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc
    17041 ggcagcacgc gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt
    17101 ctaactcgcc gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt
    17161 cttccacgcg gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg
    17221 ggttattcac acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc
    17281 agttcaggta ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc
    17341 acgccaccgg gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg
    17401 cttcatactg tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca
    17461 agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt
    17521 ttttcaggaa ctgggtcata aactgttcgg ttttggcttt atcgccaccg tcagctttat
    17581 ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg
    17641 ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt
    17701 tgcccttacg caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc
    17761 gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca
    17821 cctggttata agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg
    17881 atgacccggc atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt
    17941 gctgttcaaa cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt
    18001 tcagcagttc cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg
    18061 agttcttttt cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa
    18121 tagccatcat atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat
    18181 ttggaagtta aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt
    18241 ggacacggac agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact
    18301 gcccggggcc aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc
    18361 cccacacatg cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca
    18421 gtgtgctgcc gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg
    18481 cgaggaaggc cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg
    18541 cttttgccat ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc
    18601 tttccgccat tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt
    18661 gcgcgtactc caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat
    18721 acgccgtttc gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc
    18781 gaccatcttt gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt
    18841 taatattgat atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca
    18901 gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc
    18961 gtttgatagc tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc
    19021 gatgaccacg accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca
    19081 agattaggtt cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca
    19141 atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt
    19201 taatacagag cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc
    19261 ggatcagctc actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag
    19321 gcgttgtgtt agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt
    19381 gcacaagacc aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg
    19441 acgagctacg ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc
    19501 gcagcggaga gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg
    19561 cttctgcgca caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat
    19621 cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca
    19681 cgcgatagcc aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc
    19741 ccaggcccgt tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc
    19801 gcgcgtagtc gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt
    19861 tgcgggcaat cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg
    19921 tggcattcgt tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag
    19981 taaatccttg cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga
    20041 gctgtttcgc catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa
    20101 agtcagcaat tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca
    20161 ggtacaacat cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac
    20221 gctcttcaca ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata
    20281 tatgattcca tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac
    20341 tagaaaagat gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga
    20401 cgctggaaat gtgccggatt atcaacgatg aagataaaac cgtaccgcct gccgttgatc
    20461 gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg
    20521 gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg
    20581 aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg
    20641 aatatgccat tcagcacgcg gtggaaggag ctgaagatag ccgggaaggt ggtgttaacg
    20701 atctgaaaaa tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc
    20761 gcacgccgta tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg
    20821 gaatttcctg taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga
    20881 ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa agcaggtaca gcgcagaaac
    20941 tggtgctcaa tatgctttcc accgggctga tgattaaatc cggcaaagtg ttcggcaacc
    21001 tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta
    21061 aaaacgccac cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca
    21121 actgtaaaac ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac
    21181 gcctggatca acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca
    21241 aagagatcag cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata
    21301 tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg
    21361 ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg
    21421 tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc
    21481 tgggcgatgc accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa
    21541 aagccaaaca aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc
    21601 cgctgatccc cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca
    21661 cggtgatgca cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga
    21721 aggtgttcag caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc
    21781 aggcatttgg cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt
    21841 ataaccccgc tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca
    21901 tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc gtgggcctgc gcccgcattg
    21961 aaggcatggt gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca
    22021 ccctgctgat caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat
    22081 tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt
    22141 tagccgggct gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt
    22201 acctcgcgtt aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag
    22261 gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc
    22321 tacgcagtca ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga
    22381 tttacggtgt caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg
    22441 cgggcggttt gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg
    22501 cctttgggcc gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc
    22561 cggcaatggc ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca
    22621 ccacgctctt tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc
    22681 tatctttctc tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca
    22741 gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat
    22801 gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca
    22861 gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac
    22921 gtttgccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg
    22981 cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct
    23041 ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg
    23101 tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca
    23161 tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca
    23221 caaacacata gctgccgccg gaatccacgc cgtctttgat aaccgccact tcgcggcgcg
    23281 tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca
    23341 cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg
    23401 ccgcctgagc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat
    23461 cgaactgggt cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg
    23521 ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa
    23581 gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg
    23641 ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc
    23701 aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg
    23761 ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg
    23821 ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc
    23881 gtccgaaggc attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg
    23941 ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca
    24001 attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc
    24061 agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat
    24121 caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc
    24181 ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc
    24241 ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga
    24301 tccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc
    24361 ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt
    24421 ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc
    24481 ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc
    24541 cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg
    24601 ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt
    24661 gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg
    24721 aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc
    24781 ctgctcacgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag
    24841 cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg
    24901 gaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt
    24961 catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct
    25021 gaattttcgg gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg
    25081 caattccacg cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc
    25141 cgtcataacc gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca
    25201 aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca
    25261 gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc
    25321 gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg
    25381 tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca
    25441 actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga
    25501 tacgatataa catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc
    25561 actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt
    25621 gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag
    25681 caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg
    25741 cggaattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc
    25801 gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg
    25861 ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc
    25921 agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc
    25981 ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg
    26041 cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca
    26101 gcaagtactg tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc
    26161 gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc
    26221 cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat
    26281 caccaacccg gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac
    26341 agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc
    26401 gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct
    26461 tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag
    26521 ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt
    26581 cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac
    26641 cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt
    26701 gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga
    26761 aaaaccgggt gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta
    26821 tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt
    26881 tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca
    26941 tcgcaacgaa cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt
    27001 cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc
    27061 aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta
    27121 ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct
    27181 gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga
    27241 gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt
    27301 cagggattgg gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga
    27361 ctgcttctcg gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc
    27421 ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg
    27481 gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc
    27541 gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac
    27601 cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg
    27661 gtaactctga tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc
    27721 ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt
    27781 ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt
    27841 atgctgctct ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg
    27901 attgtgcaaa aagttagcct gacaggtgat gacatcttca gacagcacta cgcccaggat
    27961 cgtataatcg tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg tcgctatttc
    28021 tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc
    28081 aaaccagaac gagttcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc
    28141 acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg
    28201 atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc aaagcatgtt catagacatc
    28261 cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc
    28321 gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt
    28381 aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata
    28441 acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc
    28501 tttttccagc ggccagccaa agtgggcggc cacttctcct gccgtcattc cctgacatac
    28561 tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg
    28621 ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc
    28681 gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca
    28741 acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaagcg
    28801 accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc
    28861 cgctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat
    28921 cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt
    28981 aaaggcgtta caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta
    29041 gttggtttca gacggtggcg ggacgtaggt caccagctgt acgtcggcag atttcaacgc
    29101 ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc
    29161 aagcgtgatc ccggcggttg atgaaagata agaaccggta cgcgaaacca catgcgccag
    29221 gaaggcggtg ttttctgcgt cattggccca ctggaaagct gcgccatttt caatatgtgc
    29281 aaccatcgca tccagaccgg cacgcacttc cgccgggttt ggcccaccaa gcataatcag
    29341 cacttcaccg gcagtcggtg acgggccgtg tgccgctccc gcatacagcg agcgaccata
    29401 taccacttcg accatcgctt gtttggtcgc ttcgtcagcc gcaatgtagg ttacgtcgtc
    29461 agaatcggca gaaatcagcc cgaggctacg aatatgcggc ggcaatttta gttcacgcgc
    29521 aaattcagcg ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc
    29581 taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag
    29641 catacgtttc gccaaatcca caatgacggc acagcagcac gaaagagaag caaaagtcag
    29701 ctacctggta cactaggcag cgggtcagca aaggggggca aatgcccctt ttttcatgtc
    29761 agataatcag atcattacct caataaacta ataatcacat cgcggttaca accagtttga
    29821 tagtgatagc tcaaacccag acgtggcgct ggctgcaagc aaaataagcc gcaaaaatgc
    29881 gaccccatgt ttactagccg caaaaatgcg gtcctatttg ccagaacctt ttattattca
    29941 attggttagt gcaagaaaaa gcgtgattac tgttcaagaa gtatccagaa aggtcatgat
    30001 tttgacgctg aaaaaaaata ggtgggcatt tttgcccact cactagccgc aaatttgccc
    30061 actcactagc cgcatttttg cggcccaaag taataaatac agagaaataa taaatacaga
    30121 tcagcgccaa gatttttcgt cttttcggcg gaatcatgag actagtgctt acgtcctgat
    30181 aacctccaga gcagacctta acttctgtgc agcaggacta cgctcattgc cgctcggcta
    30241 ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat aatgttaaag gactctttaa
    30301 atcattcctt gacgaattaa ctagctggct taagcggaat agcatgcagg ataaaaacaa
    30361 aaccagctta gcagtgggga gagcttcgct tcgctcgtct cctggggcca atgctcaatg
    30421 aaacgacgta aggcacggag cgctcaccat aggtagacac ataaacatct gagagcgcac
    30481 cttctatcgc gctacgcgct caataaaaac caacgacacc gccacactgc aagaccaatg
    30541 gtttttatcg cgtatacaga ccagacagga aacagcagca aagcagcgta tagccaggtg
    30601 cagacagcac actcacacga cgcacagcag gcttaagtga tcactcagga aactttatcc
    30661 ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat tcgaaaggaa tggcccatag
    30721 aacgcgatga acacacataa gcgctacact tacttaacgt cgccacagag cgattgagag
    30781 cgcttaaaat cgatgcgatt tcattaaatt tttctcactc gcctgaatgg tggcgatcag
    30841 gactgatcgc aacattgccc cgcctcgata ttgagcgcat aatagataca tacattaagc
    30901 ggaaaagaga taagtttatt gtgaaaatca gggggttaca tatcatgcgc atatcagaac
    30961 aacaacggca gatcctcgac tatctggcaa aaaacgaatc aatcaccgca cgacaagccg
    31021 cagatctcgt ttatggtggc aatgttaccc gcgtccaggt tgacgccgcc cgccgctcac
    31081 tcatgacgat ggttagtaat gggctaatca ggaaacaagg ccgcgctttc gtagctgaaa
    31141 gacatatcaa tcacgcttat agccaggacg ttgaagaatt gcgccgccag atcctttggg
    31201 tattcagcaa acagggtcag gagtatttcc tttctgaaca cccaaatttc gccgccaaag
    31261 agttaacagt aagcggattg tcgtgcgcac tggattatct tgtcggtgaa ggttgcgtta
    31321 cttacaagca gctatggcac gccctgaata cgcttgtaga agatggttta ctcattgcca
    31381 cgcgtgagaa catcgaatac tggataatgc cgcgttcagg tcgccgcaat catgcacggt
    31441 atatttcagg caacagatac agaacgccgg actttaatcc gacacaggaa gatcgggacc
    31501 gtgccgaaat gatgtgcagg gaacaggaga aaagaattat ggataagtta ttttcgatgc
    31561 agtgaacaca agcgggcgca atgcccgttt ttattgcctt attccacgcc tgacgaatca
    31621 atttgatcac acctgagaaa atgcatagcc agaagttaag gccgttatat tgcgattggg
    31681 agcgttttaa acggtatcaa tttaattaat tctgatgtgg tgatttgcag gcaaaaaaaa
    31741 cccgccggag aagcgggaaa tagagacata tcgttgtagt tattaacaga tcactttgag
    31801 gtcgagggcg gctaaccctc gctaatatta tcccgctatt accttgcggg tgcaaataat
    31861 cttttattgc tttgatgatt taataaatag cactttttgc taacaaaaac tgctgtcaat
    31921 atatggccaa aaatgtcaac agaaaaaata aggtttataa tattaagtat cacatgggta
    31981 atgggttcag ggaatcggaa tccaccgcct cacggaaatt caaaatttgc ttcttctttc
    32041 ctccgtgggc ggcttctttt tcgtatcgag gtatttatga aagcaataac attagaacag
    32101 ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc gctggctaaa aaaaccatca
    32161 accggagtcc ctttgccatt taccccacgc ggtactcgcc aattattctg ggagcacgaa
    32221 tgcaaagaat ggttgtacag aacagaacga taataatcac gcatcacatt ttgaggtaaa
    32281 catcatgacc aatatcgaaa gattagaacg ctgtgccgat attatgcggc gcagatggat
    32341 ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca aaagacgtta tcaaaggcag
    32401 catgaccaat aaagggcatt tggttgtaac ggtacatgaa ggtaaattca aagtgacact
    32461 cacctatcag aaagcctgct atgtatacac atacggcgct tatgacgaga cattatatga
    32521 agtgcaccac gttaatttga acaaacagga taaccgcatc aagaatattc gcctgatgcc
    32581 gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa cttattcggc tgggccacac
    32641 acaatggctt caaccgcaat taattgaaca atcactataa acacaggagt aaataccatg
    32701 cattcattag acagttattt tcagcgcacg acagccccta aatcagccgc ccaggaacgc
    32761 cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt atattgcgga caagttcgtg
    32821 gaaacggttc gtccacttgt tgatgaagta gctgataaac ttcagtctga aatgccggaa
    32881 gatatggaag gcactgcaaa gcggcgactc atttgcgaat taagtcgtcg ttttggcgtg
    32941 tcaataagcg cttttaaata atcgaaaaaa ggggggaaag ataatggacg catttgaggt
    33001 actggcaaat aaaggcgctg aactggtagc acagcgtgat aaaaccgcca acgaagggga
    33061 acgcgcactg ctaaataaac agattaaagc gatccgtatg gcccagttta aattaatcag
    33121 taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa atcgttgctg acgctattaa
    33181 agccgccgga ctggagaaac gcattgccgg gctaaaaacg aacgcagaga aaatggagat
    33241 gcacaaacag agcggtgctc cgaatcccga tgagtacttc tcaccggatg ttgagtttat
    33301 ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag gaacagcgtc gctattttga
    33361 tggagtggat agttcggcag gtattgacct gaactcgtat ttcggtcgtg aaattgagca
    33421 ttttgatgcc gccccgtcta tgccggagcc agaaccagaa atggccaccg atcctgaagc
    33481 tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg tactgatcgg ataaaagaaa
    33541 gccgggaatt accccggctg acttaacaat gtgaccaatg aacaaatcgg acaagacaaa
    33601 caggataagc agtctcgaaa caatgttcgg gaactatatt agcacaagtc cggagaaaat
    33661 catcatgttg gcactacgca tcatgcaggg catcgctaag accctcgcag aacatgtctt
    33721 agacctgaaa cattcgccac tgagcaaaca ggccatgaag cgccaaacgc ttcgtctgtg
    33781 ggctgaatat tcgcttggaa ctatcaataa aatcatcgac atgaaatcgg ggccgagcaa
    33841 ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt ctcattcgca gagacattca
    33901 tagccagtta cacagcgtgg gaatcgatat taatgatggg acgggcgatt aattttcgga
    33961 caagtactat gcgcgcgcga ggagctgaac atgaagcaag atattgcaga caggctggag
    34021 atactcgaag gccagcgggc agaagcgaaa caactacgta agcaggccag aagagcgcac
    34081 aggaacaacg aagcagagtt actaaccaaa tacatcagtt tcaccaatta ctgcatctac
    34141 gagtgttaca aggaagatgc agaggactgg ctggattcat tgccagaaca gtactgagca
    34201 gaataacacc acgtcatgtt gttctgaggt gagcatatca cctctgttca ggtggccaaa
    34261 tttagtgtgc cacttcagca tcaccgttga aatcacaata acgatttttt tgtaaatatg
    34321 ctctcacata tgtaggaata gaccaggttg gcattgaaac acatctacca tgaccataat
    34381 aaagtaattt tttgatatgt ttatggtttt atattaatta aaatatatct aaaattgata
    34441 gttttaaaat tgcaaaatcc agattgtgtt tgtaatgttt aatctgattg tattgagtgg
    34501 cgtttattat gaaaaataaa tcaggtaatt cttgttatag agaagcagtc gatattcata
    34561 gctcacttga gctttcattt atattgcata aatatcattt gcgtgtagat gagaagaaat
    34621 ctcgaaatgt attcagcgcc atacgtgatg agctcatcct tgatggggat tcacgacaaa
    34681 acttagctac tttctgtcaa acatggcttg atgatgaaat ccgtgagttg cttaatttag
    34741 ctattgataa aaacatgatt tataaagatg aatatccaca aatggcggaa cctgaaggtc
    34801 gctgtgtgcg tatgctttcc gattcgtgga gtttccccga ttcgcgacac accttaggtt
    34861 gctcaaccat tggttcatct gaagctgcta tgctcggccg cttggcgtta aaatggcagt
    34921 ggtgtaaaaa acgcgaagta caaggtaaat caaccgaacc tgatttgcga tcctgtgcaa
    34981 atatgctggc ataaattctg ccgttactgt gatgtgaaat tgtgtgaaat tccacttgat
    35041 gatcatcacc tgataatgaa tgctaaagag gttttgaagc atgtcgatga aaacactatt
    35101 ggtgtcgtta cgactttagg tattacttat ttttgtaaag cagattgcgt tagtgttttt
    35161 aagataagtg ttaaagttaa tattagtttt tatatttaca tcgtaattat atgtttctat
    35221 tttaaaaatg ttcgagagta taaaaatgtc taatgctaat atcgcaccca aaagtaagaa
    35281 aatgacagtg gggacactcg ctatcatgaa tataactgct gtagtaagtc tacgatttat
    35341 accttctgaa gcagaatacg gcttaggtgc cattttttac tatacctttg ctgcaattat
    35401 gtttttagta cctgttgcct ttgttgcggc tgagctagct acaacatatc cacaaaaagg
    35461 gggggcattt cgatggatag gagaagcatt tggggctcgc tggggttttg taggaatgct
    35521 tatgacctgg ctacaagtca tcccctactt tccaacagta ctgacatttg gtgcagtctc
    35581 ggttgcattt attgatgttg atatgggggt ggcagaatca attgccagta ataaatggta
    35641 tattttattt ttcgtactat ttgtttactg gggagctatt tgtgttgcat tacgtggcgt
    35701 tggtattttc tcgcaggtat ctaagtggtg cggtattata gggacaatta ttccagctat
    35761 tatccttgtt attcttggtt tctcctatct gtggttcagc ggtaaaccag cggtgatccc
    35821 attaggttgg ggtgatcttt tccctaatat agctaacttc cagaatattg ttcttgccgc
    35881 cagcatattt cttgcttatg cagggatgga aatgaatgcg gtccatgtta acgatttaga
    35941 taatccaact aaaaaatacc caattgcaat taccatcgct tctataggta ctattgcaat
    36001 attcctgctc agtactttag gggtcgcctt tattattcct acagataaaa ttagtctaac
    36061 tcaaagtttg ttggttgcct acgatatgct gttcgaatgg gctggagtgc catggcttgg
    36121 ctctgtgatg gcatttatgc tagcaattgg cgtactgggt ggggtggtta cttggattgc
    36181 agggcctaat acaggtgtat tagcgatcgc gaaagctggt tatttaccta agtttttcca
    36241 aaaaaccaat cgtcatggaa tggggcacca tctaatgttt gtacaaggta taattgtctc
    36301 agttctttct gttacttttg tgataatgcc ttcagttcaa gcagcttttc agattttatc
    36361 acagttaaca gttatgctct atttagttat gtatatgctc atgttcgcta gtgctatcta
    36421 tctacgacat tctcaacctg atacaaaacg cccatatcga gcccctgctt tatttttctg
    36481 ggcaacactt ggattcttag ggtcttttct ggcttttgta ttaagtttta ttccgcccga
    36541 gcaaatccca actggtagtc caaccagcta tgttctttat ttgttagtat tagttgccct
    36601 atttttatta attccaatat taatttacgc cttccgaaaa tctgactgga aagatccaaa
    36661 ctctgatttc gaaccatttt cttgggaaaa aagaactagt aattaaattt tccataagct
    36721 acaaaagatg aggttaaata tgtttagtgc tgaatctcca attggtgtaa tagactcagg
    36781 tgtaggtgga ttgacatcgg taaaggaaat aattaatttg cttcctggtg aagatattat
    36841 ctattgtgga gataatatga atgcacctta tggcaaccgt tctgccgatg atatcatcag
    36901 tttgactaaa aaaatgctga cttttttaca gtcaaggaat gtcaaactag ttgctgtcgc
    36961 atgtaatact atatcctcaa ctttggaatc cgaagaatac agcggatatg ctaagagctt
    37021 tccgtttcct atcttatcca tcattgaacc tgcagtagaa gatgtgattc gccagcaata
    37081 taaaaatgtt ggtattatcg ctactgagtt tactattaaa acggggtgtc acaaagagct
    37141 aataaaaaaa ttgaattcaa caataaatgt atttggagaa ccaagcaaaa atcttgcaat
    37201 gttaattgag gaaggtaatc ttaatgctcc tgcaatcttg aatgacatca aaaaacatgt
    37261 taaccattta atttccctcc atccagtaaa tgaaattatt ctcggctgta ctcactatcc
    37321 aattgttcag aatttttttg aagcagttgc accggatata aaatttatta atcctgcaca
    37381 tgatcaggct atctctatta aaaatcactt gggacagcta aatttattaa actcctcaaa
    37441 tataggaact ttgcaaataa atactagtgg aagtatggag atctacaaaa cggtactagg
    37501 ggaattgagc attacgaaac ctcatacatt cagtatcagg cagttttaaa tttatccggt
    37561 gcaccgtaaa atcccgtcct tctgaactgc accccaaaag ttggacacca actgagtaag
    37621 gtgcatttta tggacaaacc cagttacata ctgggtagtg catccaatta gtagaacatg
    37681 tgtttttcga taaacgctcc gatcactttt tcgtggatct caactgagcg tgagaagcag
    37741 attgttttac gacccaaccg cttaatgcgg gtgcgtagcg tcagattatt acgctcaatg
    37801 cgttgggtga atattttgcc ggtcagatgc ttattcttcg gcacctcccg gccatagctg
    37861 ccccagtcat cgctggtcag catgccgatg ttgaagggtg taagcagtgc cagtagctcc
    37921 cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca gtacaccgcc tgttttggtg
    37981 ttgtacgcgt acccgagcca atgttgccgg gctttactgc caacgtagct ccattgctca
    38041 tcaagctcgc agataaacgc cacatcagca tgggcaacag gcgaagaagt tattcgcttt
    38101 ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc aattttcagt gtcctggcgg
    38161 tatcgcgaac cccggcacca ttaaaggcca tttcagttat cagctctttc atacccggct
    38221 tacgtgcttg ataagtataa gtgaactgaa acacacggtg gcagtcacgg cagcgaagtc
    38281 tgtcacggcc tttagggttc tgaccatggc ggtaaacctg agctgactga caacggggac
    38341 aatgaatatt aacgctggtc atgagataac ctcaaaagcc cgtattatac atcagattca
    38401 actaattaga ggcatcaccc atccagcatt ataaaacata ctaacaataa ctaaattata
    38461 ataatcacaa cttacaatca aataataaat gcataagatt aacaatgatt ttttcgggtt
    38521 atatttatct aaaaaagcag aatattatgt aacttattat tttctaagcg aaaatttatt
    38581 gtgcggtcag actaacaaac ttgcgtacca cccgaagaac atgcgaagat ttaagtattc
    38641 tttctttttt ggtttttatt tttcattttc tcttttcttc tctttccggt tctgttctgg
    38701 ttttggtttt ttccttttta tttttattct ttcttcaggg ttatgctaaa acaccaaaac
    38761 atttttcacc acaaaagcat ccaatcatct tgccagacca atcaccttaa ccggatacac
    38821 tgccggatat aatcccacta acacccagac accaagcacc atacctgata actaccgtat
    38881 tcaggaagga aatgcgagtg atttcaccgt caccggcaca accgatccgc caaaaagagg
    38941 cgtaccaatg tcgatatagt cccccgcgcg gacaatcact tcgtcaatga ctgccaacgg
    39001 gagttgttgt agctgtgggc gcaacaacat gcccagagct ttaccaaagt cctgcccggc
    39061 caccaccagc aggggatgcg gattcggaaa acgcgcgacg aaatcgacca gcgcgttgat
    39121 gaccgtcaga accgcggcgt aacgcacagg cagcgaggcg ggaagcgcca gcacgtacac
    39181 gtcagttttg ggatcaagat ccagctgaag cagcgcctgt tgccaggcac tcaccagatc
    39241 cgtttcatca atcgggatcg ccaccggcaa attgcgcagc ggcagttgta cgccctccag
    39301 ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc gcgccaatca ccgtggagcg
    39361 tacggtttgc gccggaaact gcacattcat ctcacgcagg cgcggatggt catgcagcgc
    39421 cgttgccaga agtgggccaa tatcggcaaa acagaacggg ttggcgggct ggtggcgata
    39481 acattcgccc acgcctccag aaagcgtaat gatttcgggc gtaacacctg cgggcagcaa
    39541 accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt ccgtcaatca cttcgacaat
    39601 cagcgccgcc atccgccggg taacctgcac cagctgcgcg ccggtcagcg aacgggcgtc
    39661 agtgcctgca ccgaagcact catccacaat catctgcccc ggtttatgag cgtaaaccac
    39721 gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg ttgagacagg cagtgccgct
    39781 gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca ccgccgatat cgatattcag
    39841 tacccgacac agccgttgtt cagaaagggt ttgcgccccg gctccgtgac cggcgatcac
    39901 ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg cccagcgact gtttttcctt
    39961 catcactttt tccatcagct gtgcgaccag gctttcggta aactggcctt ccggcagctg
    40021 ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg tcggctggca cgctggcggg
    40081 cacggacgcc gttaccggga tcgattcatc acattcggtg atggtaaaac ccagtagatc
    40141 cgccacttca cgggcttccg gggtaataat gctggcgcgc agaaccacgg acattgcctg
    40201 ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg atgagttttt tcacctgctt
    40261 cctcctgttt ggtgagagat gccagataat ccaccagttg ctgcacgctt tgcgggtcgt
    40321 ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc aagcaataac tttcgcgttg
    40381 cggcgacgtc agcatctggc atgtccgttt tactgatgac ggcgatttgc cgtttgctga
    40441 cgccaatatc cagcaacccg gcaggcaacc gactttccgg gtcatttgcg ccatggacat
    40501 agatcagcat gtctacatcc tgtagcgtgg taattaaggc gtgataccag cggggatggc
    40561 taaaatattc acccggcgta tcaatatcgc ccttatcatt aaattccacg gccagtgttt
    40621 ttctggcgag ggtataattc ccctgcaacg cattaaataa cgttgttttt cctgccccga
    40681 cgctacccac aaacgcaatt cgtttcatga atg
//
LOCUS       DADLDT020000022        40315 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000022 DADLDT020000000
VERSION     DADLDT020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40315
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.022"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="JQH22_001119"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="JQH22_001119"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFB2205516.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JQH22_001120"
     CDS             complement(1672..2730)
                     /locus_tag="JQH22_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFB2205517.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JQH22_001121"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JQH22_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JQH22_001122"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JQH22_001122"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFB2205518.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQH22_001123"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQH22_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFB2205519.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQH22_001124"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQH22_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFB2205520.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JQH22_001125"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JQH22_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFB2205521.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JQH22_001126"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JQH22_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFB2205522.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQH22_001127"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQH22_001127"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFB2205523.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQH22_001128"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQH22_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFB2205524.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQH22_001129"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQH22_001129"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFB2205525.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQH22_001130"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQH22_001130"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFB2205526.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JQH22_001131"
     CDS             complement(17699..18322)
                     /locus_tag="JQH22_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFB2205527.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JQH22_001132"
     CDS             18619..18894
                     /locus_tag="JQH22_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205528.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JQH22_001133"
     CDS             complement(18971..19213)
                     /locus_tag="JQH22_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFB2205529.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JQH22_001134"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JQH22_001134"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFB2205530.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JQH22_001135"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JQH22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFB2205531.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQH22_001136"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQH22_001136"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFB2205532.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="JQH22_001137"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="JQH22_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="JQH22_001138"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="JQH22_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFB2205533.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="JQH22_001139"
     CDS             24840..25697
                     /locus_tag="JQH22_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2205534.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQH22_001140"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQH22_001140"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFB2205535.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQNILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQH22_001141"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQH22_001141"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFB2205536.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQH22_001142"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQH22_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="JQH22_001143"
     CDS             complement(30355..31602)
                     /locus_tag="JQH22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFB2205537.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="JQH22_001144"
     CDS             complement(31624..31950)
                     /locus_tag="JQH22_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HFB2205538.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQH22_001145"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQH22_001145"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFB2205539.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="JQH22_001146"
     CDS             33338..34594
                     /locus_tag="JQH22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFB2205540.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="JQH22_001147"
     CDS             34709..35035
                     /locus_tag="JQH22_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFB2205541.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="JQH22_001148"
     CDS             complement(35176..36414)
                     /locus_tag="JQH22_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFB2205542.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="JQH22_001149"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="JQH22_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFB2205543.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="JQH22_001150"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="JQH22_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10752 a        10025 c         9312 g        10226 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaagtca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tatcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatatttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccctat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaggt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagctaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgacg
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctggtt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttg
//
LOCUS       DADLDT020000023        39986 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000023 DADLDT020000000
VERSION     DADLDT020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39986
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.023"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(94..1347)
                     /gene="amiC"
                     /locus_tag="JQH22_001151"
     CDS             complement(94..1347)
                     /gene="amiC"
                     /locus_tag="JQH22_001151"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFB2205544.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1579..2910
                     /gene="argA"
                     /locus_tag="JQH22_001152"
     CDS             1579..2910
                     /gene="argA"
                     /locus_tag="JQH22_001152"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFB2205545.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2972..4798)
                     /gene="recD"
                     /locus_tag="JQH22_001153"
     CDS             complement(2972..4798)
                     /gene="recD"
                     /locus_tag="JQH22_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFB2205546.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4798..8340)
                     /gene="recB"
                     /locus_tag="JQH22_001154"
     CDS             complement(4798..8340)
                     /gene="recB"
                     /locus_tag="JQH22_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFB2205547.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8333..11221)
                     /gene="ptrA"
                     /locus_tag="JQH22_001155"
     CDS             complement(8333..11221)
                     /gene="ptrA"
                     /locus_tag="JQH22_001155"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFB2205548.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11397..14765)
                     /gene="recC"
                     /locus_tag="JQH22_001156"
     CDS             complement(11397..14765)
                     /gene="recC"
                     /locus_tag="JQH22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFB2205549.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14778..15110)
                     /locus_tag="JQH22_001157"
     CDS             complement(14778..15110)
                     /locus_tag="JQH22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFB2205550.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(15095..15502)
                     /locus_tag="JQH22_001158"
     CDS             complement(15095..15502)
                     /locus_tag="JQH22_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFB2205551.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15499..16062)
                     /locus_tag="JQH22_001159"
     CDS             complement(15499..16062)
                     /locus_tag="JQH22_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFB2205552.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16053..16523)
                     /locus_tag="JQH22_001160"
                     /pseudo
     CDS             complement(16053..16523)
                     /locus_tag="JQH22_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16707..17501)
                     /gene="thyA"
                     /locus_tag="JQH22_001161"
     CDS             complement(16707..17501)
                     /gene="thyA"
                     /locus_tag="JQH22_001161"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFB2205553.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17508..18383)
                     /gene="lgt"
                     /locus_tag="JQH22_001162"
     CDS             complement(17508..18383)
                     /gene="lgt"
                     /locus_tag="JQH22_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFB2205554.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPDSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18534..20780)
                     /gene="ptsP"
                     /locus_tag="JQH22_001163"
     CDS             complement(18534..20780)
                     /gene="ptsP"
                     /locus_tag="JQH22_001163"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFB2205555.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20793..21323)
                     /gene="rppH"
                     /locus_tag="JQH22_001164"
     CDS             complement(20793..21323)
                     /gene="rppH"
                     /locus_tag="JQH22_001164"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFB2205556.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINLQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22008..22697
                     /gene="mutH"
                     /locus_tag="JQH22_001165"
     CDS             22008..22697
                     /gene="mutH"
                     /locus_tag="JQH22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFB2205557.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22766..23479
                     /locus_tag="JQH22_001166"
     CDS             22766..23479
                     /locus_tag="JQH22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFB2205558.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23617..23835
                     /gene="ygdR"
                     /locus_tag="JQH22_001167"
     CDS             23617..23835
                     /gene="ygdR"
                     /locus_tag="JQH22_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFB2205559.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23943..24983
                     /locus_tag="JQH22_001168"
     CDS             23943..24983
                     /locus_tag="JQH22_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFB2205560.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25016..26206)
                     /gene="lplT"
                     /locus_tag="JQH22_001169"
     CDS             complement(25016..26206)
                     /gene="lplT"
                     /locus_tag="JQH22_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFB2205561.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26199..28358)
                     /gene="aas"
                     /locus_tag="JQH22_001170"
     CDS             complement(26199..28358)
                     /gene="aas"
                     /locus_tag="JQH22_001170"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFB2205562.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28944..29975
                     /gene="galR"
                     /locus_tag="JQH22_001171"
     CDS             28944..29975
                     /gene="galR"
                     /locus_tag="JQH22_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFB2205563.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29982..31244)
                     /gene="lysA"
                     /locus_tag="JQH22_001172"
     CDS             complement(29982..31244)
                     /gene="lysA"
                     /locus_tag="JQH22_001172"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFB2205564.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31366..32301
                     /gene="lysR"
                     /locus_tag="JQH22_001173"
     CDS             31366..32301
                     /gene="lysR"
                     /locus_tag="JQH22_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFB2205565.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32288..32980)
                     /gene="ygeA"
                     /locus_tag="JQH22_001174"
     CDS             complement(32288..32980)
                     /gene="ygeA"
                     /locus_tag="JQH22_001174"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFB2205566.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33109..34527)
                     /gene="araE"
                     /locus_tag="JQH22_001175"
     CDS             complement(33109..34527)
                     /gene="araE"
                     /locus_tag="JQH22_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFB2205567.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34842..35603)
                     /gene="kduD"
                     /locus_tag="JQH22_001176"
     CDS             complement(34842..35603)
                     /gene="kduD"
                     /locus_tag="JQH22_001176"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFB2205568.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(35633..36469)
                     /gene="kduI"
                     /locus_tag="JQH22_001177"
     CDS             complement(35633..36469)
                     /gene="kduI"
                     /locus_tag="JQH22_001177"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFB2205569.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36756..37937)
                     /locus_tag="JQH22_001178"
     CDS             complement(36756..37937)
                     /locus_tag="JQH22_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFB2205570.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38192..39421
                     /locus_tag="JQH22_001179"
                     /pseudo
     CDS             38192..39421
                     /locus_tag="JQH22_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         9921 a        10814 c         9704 g         9547 t
ORIGIN      
        1 aatccggccc ccgcaaccaa ttattgaaca ccctaacggg tgtttttttg tttctgatct
       61 cccataaaaa aagcgccatt cagcgccttt ttatcatccc cttctcgcca gcgttgcccc
      121 atcggcaaaa tacgctttaa tccccgcaag aatagactcc gcaacttcct gctggaaagt
      181 cgccgttttc agtttacgct cttcctcaac gttactgata aacgccgttt cgaccagaat
      241 ggagggaata tctggtgcct ttagtacggc aaacccggcc tgttcaactt gatttttatg
      301 cagcttgttg atttttccga gcttattcag taccgcttta ccaaacttaa ggctgtcagc
      361 aatagtcagc gactgcacca tatcgaacat ggtgtggtcg acatagcggt caccgctttt
      421 gctcacgcca ccaatcaagt ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt
      481 actggttgcg cctttggttg agagcgcaaa caccgaggaa ccgctcggct gacgactggt
      541 aaaggcgtcg gcatggatag agacaaacag gtcagcacgc tgtttctggg cttttgctac
      601 gcgcacttgc aacggaatga agatgtcttc attgcgcgtc atgtacacct tcatattgcc
      661 ctcttcctcg atcagagagc gcaaacggcg agctatttgc aataccacat ctttttcgcg
      721 cgttttgtat ttccccaccg caccggagtc ttcgccaccg tggccagggt caagcataat
      781 gacaatcgga cgatcgcgcc ctgctttacc cggttgtgga ccactttgcg ctggcggcac
      841 ctgcttttcg aggtcgcctt tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat
      901 atcctgtgca ttggccggat agaggtccat caccagacgc tctttaaacc cggcgaccgg
      961 cgcaagggca aacagctgcg gttttacgtt ttgctttaat tcaaaaacca tgcgtacggt
     1021 ttgcgggtca aattgcccga cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg
     1081 tgccgccatc cccttgagta ccgagttcag gtttacatct tcgatatcca ccaccacgcg
     1141 ttcagggtta ctcaacgcga actgcttata tttcagctga cgatttgatt ctaccgtcac
     1201 gcgggtgtag ctggacgcag gccagacgcg caccgccacg acctggctaa ccgcagccag
     1261 gctgacctga cttacgctca atagccacat agcgcccgcg ccttgcagta aacgacgacg
     1321 gctgattgca gtgttggatc ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt
     1381 aacaagtaaa cgactaattt gaccggaaac tttagcgaaa gacgcataat ctgtcatcta
     1441 ataaacggta aacattcttt ttatattcac ggcattactg ataaaaaagt cgctctcgca
     1501 taaaatttac acttgcaccc tgcgaaaaaa cagaataaaa atacaataat ttcaaataat
     1561 catgcaaagg ggtgtgccgt ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc
     1621 cattcggttc cctatatcaa tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt
     1681 gaagccattg agcatgagaa tttctccagt atcgttaatg atatcgggtt gttgcacagc
     1741 ctcggcatcc gtctggtggt ggtctatggc gcacgtccgc agatcgacgc aaatctggct
     1801 gcgcatcacc acgaaccgct gtatcacaag aatatacgtg tgaccgacgc caaaacactg
     1861 gaactggtga agcaggctgc gggaacattg caactggata ttactgctcg cctgtcaatg
     1921 agtctcaata acacgccgct gcagggcgcg catatcaacg tcgttagtgg caattttatt
     1981 attgcccagc cgctgggcgt cgatgacggc gtggattact gccatagcgg gcgtatccgg
     2041 cggattgatg aagacgcgct ccatcgtcaa ctggaaagcg gtgcaatagt gctaatgggg
     2101 ccggtcgctg tttcagtcac tggcgagagc tttaatctga cctcggaaga gattgccact
     2161 caactagcca tcaaactgaa agctgaaaag atgattggtt tttgctcttc ccagggcgtt
     2221 actaatgacg acggtgatat tgtctccgaa cttttcccta acgaagcgca agcacgggta
     2281 gaagcccagg aagagaaagg cgattacaac tccggtacgg tgcgcttttt gcgtggcgca
     2341 gtgaaagcct gccgcagcgg cgtgcgccgc tgtcatttaa tcagttatca ggaagatggc
     2401 gcgctgttgc aagagttgtt ctcacgcgac ggtatcggta cgcagattgt gatggaaagc
     2461 gccgagcaga ttcgtcgcgc aacaatcaac gatattggcg gtattctgga gttgattcgc
     2521 ccgctggagc agcaaggtat tctggtgcgc cgttctcgcg agcagctgga gatggaaatc
     2581 gacaaattca ccattattca gcgcgataac acgactattg cctgcgccgc gctctatccg
     2641 ttcccggaag agaagattgg ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt
     2701 tcatcacggg gcgaggttct gctggaacgc attgccgctc aggcgaagca gagcggctta
     2761 agcaaattgt ttgtgctgac cacgcgcagt cttcactggt tccaggaacg tggatttacc
     2821 ccggtggata ttgatttact gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa
     2881 tccaaagtgt tgatggcgga tttagggtaa gcgatgaaaa tcaccggatg cggcaggcgt
     2941 aacactcgtg cgtcgcatcc ggcaattacg tttattcccg cgaactaaac aacgccgcca
     3001 gaccactgcg ccgctcagta cgagtggcga ttgccgcact taatatacgc tcatcggcat
     3061 acagcgacag acgacgacgc gcgcgggtca ccgcggtgta taccagctct cgcgttacta
     3121 ccggcgtgcg ttggctcggt aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt
     3181 tatgtaccgt catcgcccac gtagtttcgt gctctggcaa gcgactcggt tgcacagact
     3241 taatattgcc gtccggcatc gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa
     3301 taccgatatc gccattaaac aacccaagcg cgctgtcatt acgggcaatc atcaccggtc
     3361 ggccttcgta ccaacgagag tgcggatgac gatgaatttt gcgcttctgt tgcataaact
     3421 gttcaattcg ctcattcagt ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa
     3481 gctggtactc attgaacgcc tgaatgatta aatccggctc ggcacgcgct tgcagcagat
     3541 ccagataacg tccgtaaccc gcaagagctt cctcaagcat cgcaatataa tcttcgccgc
     3601 tctgtaaaag tcgtttttcg atatcagtaa aatcctgctg aaaaacggtt ttcactgccg
     3661 ttttatcacc acggttgatc gccgcagcta actgaccaat gccagaatcg ctgccgaaac
     3721 gatagctttt ttgcagcagg cagagactgt cgcgcaaaga tgccgcttct gtgccggttc
     3781 ctgccggaac gtgagtcccc gtcaggcggc ttagctgcct ggcacgctcg gcggtaaagc
     3841 ccgcgttggc ataagcgcag atatcgccca gcacagcccc agcctcaacc gaggccagtt
     3901 gatcacgatc gccgagaaag atcactcgcg catgatctgg caaggcgtcg atcagtctcg
     3961 acatcatagg cagatcgatc attgacgctt catctaccac cagcacatca agatgcagcg
     4021 ggttaccggc atgatgacgt aaacgctggc tacccggctg cgcgcccagc aatcggtgca
     4081 aagtgctggc atcttccgga atgcgtttct tttgttcatc ggtcagcggt aactgtcgca
     4141 aagccttgcc gagagattcg gttaagcgcg cggcagcttt acccgttggt gcagccagac
     4201 ggatacggca gcgttcgccg tcggccattt gaattaacgc tgccagcaac ttcgccacgg
     4261 tggtcgtttt accggtgcct gggccgccgg aaatcaccga gatccgccgc gtcagcgcca
     4321 ctgccgctgc aactttttgc cagttaattt catcgcttac tggaaaaagt ttgtccaggg
     4381 tttgcgccag tagagcttca tcaacctcaa tggtatgatt cacctcattg aagaagcgcg
     4441 ctaccgtccg ctcgttacac cacatgcgat tcaaataaag acgatcgcca cagaggatca
     4501 tcggcgtggg ttcatctccc ctgctgaccg cttgagaagc cagcaagcat tcttcccaat
     4561 tttgtagctc accgatttca ctgacacagg tcgccaacag cggattcgac gcctcgttat
     4621 tttccagtcg tgaaagcggc aaacagacgt gtccctctcc ggcatcatga cttaacagtg
     4681 ccgccgcgag ggtgacggca ggatgttcat ctcccgccac ggtcagggca aattgcacat
     4741 ccagcgggcg tagctgtttg tgctccacag cttccagtaa ttgcttttgc aatttcatta
     4801 cgcctcctcc agggtcatac cggcaaacat ctcatccatc agggcaatca acccggcgtt
     4861 gggtcgggtt gtgtaaatcc cttgttgcgg atgttcttta tcaacgccac gcaggaacag
     4921 atagataacg ccgccaaagt gaagatcata gtcgtaatca gcaatgcgat ggcgcagata
     4981 acgatgcagc gccagggtat aaagctgata ttgcagatca tagcggtgtg cctgcatcgc
     5041 tgctgccata gcctgttggg tgtaagccga actgtcttca cccagccagt tggatttata
     5101 gtcgagcagg taataacgcc cttcgtggcg gaataccagg tcgataaagc cttttaacat
     5161 gccgcgtacc tgcatgaact ccagcggcgg gcagccagcg gatagcgggt caaactggcg
     5221 aattagtgca tcaagctggc tggcgatgag cggttcgcta atcggcaggt aaaactccat
     5281 ctccacctgt ttatcgcggt cggaaagctg actcaggcta acacccgttt cattgagagg
     5341 tgcctggagg acagccgtga tccactcggt caacaccggt tcccactgcg attcaaagcc
     5401 gccgagttcc agtttttcct gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc
     5461 ttcaaacaaa ctgtgcaaga atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt
     5521 taacgtcggt tcttcaacga cgctgaccac gcctgcggca tcaacatcca gccgtggcat
     5581 cagatcctga gcgataccgt gaccacgctg ctgcaagccg gagtagctgg tgacacgcca
     5641 gttatcgccg ggcaatcgtt gtagtgtcct cgcattcagt tctgcagtta aagcatcatt
     5701 aacctgccag ggctggttat caccagtttg tgccgtttgc caggcaatat catcatcgca
     5761 taacgcttca atacaggtgc gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa
     5821 acgcccgagc gcactttggt ggacgtcggt gtcacctttt ttatcgccac gacggcgcac
     5881 cagcggtgca acgccgagac tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa
     5941 acgcagatct tccgccagac gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt
     6001 aagatccaaa actgcctcaa acgagtggcg atcgtgataa aacgcctgat cctggacgcg
     6061 gaaattggta ataaatggca accagaccag cggatattcc agccctttcg atttatggat
     6121 cgtgacaatc tgcaccagat gtttatcact ttcgaggcgc atttgttggc tggaggcatt
     6181 actgtctggc tcgaggatat gttgcgataa ccagcgtacc agcgcatgtt cactttccag
     6241 ctgcgttccg gcttcttgaa gcagttcgct gatatgcaag atatcggtaa gacgccgctc
     6301 accgcctgcc gttgccagca agttttctgc aatgttacgc gccgacatca gcgcccgcag
     6361 catcggcata acgccacgtt tgcgccagat ttgccgataa ccatcgaact cttcgactac
     6421 cgcatcccac gcatgttcgt cattattcag cgtttcaata tccagcgcat tcagccccat
     6481 cattgacgtt gccagcgcac tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg
     6541 caacaaccaa agcatttcct gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag
     6601 gtaaacggaa gggatttcca gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg
     6661 gctgcgcacc agcacgctga tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat
     6721 cagcaacgct tcgccccgct gtccggcttg tagccagtcg cggatttgcg cagcacatac
     6781 ctgcgccatg gtactttgat agtcgccaac gccgcagctt tcgccttcca tcagccacat
     6841 tttcatcgca ggctgcgttt cacctttaaa tacaaaacgt aacgcctgat ttttcccggc
     6901 tgatttcact ggaataaacg gtatttcgcg aaacatgaac gtgtcatcag tctggctgaa
     6961 aagcttgttc acactgttca ccattccagg tgcggagcgc cagttggtgt ctaaagtgta
     7021 gtgggcgtga acttcactac gcgccttcat ataagtgaag atatccgcac cccggaatgc
     7081 atatatggcc tgcttcgggt cgccaatcag caacaatgcg gtttccggct gatggtgcca
     7141 gatacggcga aaaattcggt actgctgggg gtcggtatcc tgaaattcat cgatcattgc
     7201 caccgggaat cgcgtacgga tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc
     7261 ggaatcgagc cgacttaaca tgtcatcaaa acccaattcg ccacggcggc gtttttcacg
     7321 cgctactgtt tcgcggatct cagccaatgc gcgggtgatc aacagatcgc ggatcgacaa
     7381 tggttctgca agcagttgat cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc
     7441 ggccttcgtg cgatcttcta agaaacgctg ggagaatttt tccagcgact ccggcaactg
     7501 ataactgttt gtctcttctt ctgcccaggc gctgatcttt tcgatccatt tagcctgatt
     7561 gctacggtta aacttgcgtc gatcaatacc agaagattcg atcagcgcat ccagttcacc
     7621 cactgcgtcg cgccactgct gttttaccgt atcaatacgc gccacaattt gcgcgtggcg
     7681 ggaagccagc gtttcatcat cgggcggcgg tgctttgata accggcggtt cgccttgcag
     7741 ataacgatta atatcgcgca gcaacgcctg cggccctttc caggtttcaa agacgacctg
     7801 ggctatttca cgcggcagcg ggtagcagtg gcgacgccag aaatcggcgc aggcctggta
     7861 gcgtagcaga gactcatctt caatcagctg ctgctcaaac agcatgccgg attcaaaggc
     7921 attcaggttg agcatgcgct ggcaaaagcc gtgaatagta aagactgccg cttcatccat
     7981 ctgccgttcg gctaacaaca accactgcgc ggcttgcgct ttatcgtcga tctcttccag
     8041 caggcgttcg tacagtggat tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg
     8101 gatattgcta cggatacgac cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac
     8161 cagcagttct tcaacgatca gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa
     8221 caggcgcaaa tagagcgccg caatcgtaaa ggttttgcct gtgccggcag aggcttcaat
     8281 caggcgctcg ccctgtaagg gcaagcgcaa aggatctagt gtctcggcga catcactcat
     8341 tcttttcact catcaggggc attgtttgct gcaacgcgct gacgttctcc cacactttcc
     8401 agccttcagg atgcacatat tcggctttcc cgttctggct gccggaaatc tgcgacagaa
     8461 tagccatgcc ttgcggctcg accaccgcct gatggaagaa atcagcaagt ttttgcggcg
     8521 tcagcagttt tatctgggcc acgattttat cacgcgaatc gaagcgcata ttgccgcgat
     8581 cgaaatcttt acttaacttc gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct
     8641 gggtaattac cgcctgctgg atttgcgcaa actcatctgg cttcatcgct cgcaattttg
     8701 cctctgcggt tgggaaaaac gccttgtaac gctcccacaa gaatgaaggc tgtttatcat
     8761 tgctttgcaa aaggaagccc atgccccact gacgccccac gctcattgga aacgcaaaca
     8821 cggcatagcc caattgttct tcggtacgca actgattgta gaaccacggc tgtacgatct
     8881 gccccaacag agagctatag gctgagctgg tgtattcatc gtagccagtc ggtacaaata
     8941 ccgctgccag tgcggagtcg gtgctgttac cggctttttc aaagatgacg gattgttttt
     9001 tatcgaccac tacatctttg tttcgacacc actctgaacc atcagcgccc aactgttttt
     9061 gcacatcgcg tgccagcgtt gttgcctggg cctcggtcat gttgccgata accataaact
     9121 ctggtcgagc ccctgatttt aaggcgtcgc gataggccag cacctctttc aacgtaatgg
     9181 agggcaaaat tttacgccgt tcatctcgcg agaagtacgg cacttgcgag agcatctgcg
     9241 cgggcataat cgcctgctca aacgctttac ccttttctgc ggaatccatc atctggttat
     9301 accaggactt cgcctgctca agctgctctt ccgtagcggt atagctaaag tacccctcga
     9361 gcaatgcctg gaacagctgc ggcagacgct gggtgtaacc attagcatta atcataaggc
     9421 cgttgttagc gttggtggaa aaacttatgc caccaaccga cgcctggttg cttaactgat
     9481 caagcgccag ccctgcgaga taatcattga gcgcaaacat cacctgattg cgggcgctgt
     9541 ccatggcttt cggattacgc aaaatcaggc tgacatcagc tttgggctcg ctggcaaaat
     9601 aacggcttgg cgcatacacc acgcgcagat tcgactcatc aacaatcagc tctggatggt
     9661 cgtatttctt ctctgactta atcagcgaga aatcatcagg aatataaggg ttaagctctg
     9721 gcaaagagag cgcaatgtcg gcggcttttt tctgccagtc ggcgaaagtt tgtgcgctga
     9781 ttttatcgac ctgatacggc gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg
     9841 ggctgatata ccagatacgc gcattctgcg gcgtcatcat cgccagacgt tcctttactg
     9901 ctttagcatc gtaccgatcg gcaatattga ctgcatccag cgtatgctca acaggaacgc
     9961 gaatcatggt atctgccagc cattcgacgt aatccatatc acgggtgatc gacggataac
    10021 ggaagtcgat atccagcaca ttcgccagtt catcgaagta ttgtttatca atgccttttt
    10081 cacgtaacag attgagatag ctaaaaattg ccgccacaac ctgatcgcga ttagccaagc
    10141 ctttatcagt taaagacgca gagatcgcta atacgccgct gttgccgttg acgataggat
    10201 cggagttggc gctaatgccc tcaactaatc cctgcttttg cagccagtca gaaagtgtac
    10261 ctgggctgcg attgccaatc agataggtaa tcaattcatc ggttttacta cggaacttcg
    10321 ctgagttgtt atcgatgcga aactcaacgc gcaacacttt acgcggcagc gcagggacgt
    10381 aatgaatgat aatgcccttt tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt
    10441 tgctctcttt gttcggcacg cgaccaaagg tgtccgccgc catttttgcc aactccggca
    10501 gcggtttatt actgtaaata accgccttca tcaaattggc ggaatagtac ttctcgtgga
    10561 aatctttcag cgcctgctgc accggattac caggtttgtc gcttaaagtt tcgaggttac
    10621 caccagaaaa ctttgaaccg gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca
    10681 tgcgcatccc gtcacgcgta cgcgccatgg ttaattcagc gttcactgca ttacgctcac
    10741 gttcggcgta tttcttgtcg agcaagggtt ctgcaatagc atcggccagg cggtctaccg
    10801 caccgggcaa ggcgtcgttc tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc
    10861 tggcattgtg actaccgccg tgcattttga gatattcggc cagactgtca gcctgcgggt
    10921 actttttcga ccccatcaga ctcatatgtt caaggtaatg tgccagcccc tggtacgcct
    10981 cgggatcttc cagcgaccca acgggcacta ccagcgccga gagcgattta actgcctgcg
    11041 gatcagaaac cagcaagacc accataccgt tatccagacg tatagcctga tactggcggt
    11101 tatctttatc acttttacgg atggtttcct gaatcggctg ccatcccgtt tctgcctgac
    11161 ttaatggtgc ccagagggca acaaacaaca ataatgcttt gaaccaggtg ctgcggggca
    11221 ttcacggacc tcataatcaa ctttattttc tggacagatt caacaggtta acgcaaatca
    11281 tctgccagaa tttaatcttg tgctgcacga gtcagcctat gtttatataa ccatcagtcc
    11341 gtgactggtg cgcatcataa agtaagcgga tagattgcgc aatttttata caacactcat
    11401 gactgattaa agcgaaacag cggtaacagg aaacgttgcg actgttcaac gatggcctcc
    11461 attgtctctg gtgttaattg ccgccagagc cgttgatacc agatatcatc accttcgcca
    11521 cgcaccatca tgttgccttc gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg
    11581 gaatcgtcat ccagcatggc atcgttttgc gcgtcataac aggtttttag ccacgcgccg
    11641 ccactttcag gtaacaccag caatggcgcg gacattcctt cacgataccc ctcaatcagt
    11701 tgtgagaggt aatgcaaagc ctgttcggct gcaagcggcg gaaaacgcca ctcaccgtct
    11761 ttgcgtagaa aaaggcgact ttcgccatta ccaccgctgg cacagtagac aaggtgttcc
    11821 agccaaagtt gcattccctg cgccacactt aataaagagg gacgccagcg caacaggcca
    11881 tccggctgca cctgcggcaa ccagccagtt atctgcacac cgttgcaggc gagatcaatt
    11941 tccatactct gccccggctg gcgacaggca atgactctgt cggcaagctg ctgcatctcc
    12001 tggcactgtg tttcccagaa aatttcacca aaagcgccat acggtaaatc ccctgccgcc
    12061 cggaagcggc ggaacaagcg ttcggcatca tcctgctcaa ccagtgcatt caataactgc
    12121 tgattgattt gataacggct aagtccttcc agaataaatg gctcggtgtc tgggatttcg
    12181 ctgtcttcag tatgaaagtt cacctgcaaa cgcatctgga agaacgcccg caccggatgt
    12241 gcccagaatc gttgtagcgt ttccagcggc acggtttccg gtaaggtaaa cggcagcggc
    12301 tgaacaaatt cagaatgtgc tttaccagcc tggctggccg caggtagcca ttcacgagca
    12361 tagctttgtc gttcgcctgg ctggtagttt tgcggatcaa acggcatccg ggtatggagg
    12421 caagtaagat gcgcttttac ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc
    12481 ggtagataat gactttgccc gatgtagtcg atcagttcct gcaccagtac cgacgggaaa
    12541 cgttcactgt tatcctgaat ggaacgcccg atatagctga tatagagttt ttgctgcgcg
    12601 gaaattaacg cttccaggaa caggtagcgg tcgtcatcgc gacggctacg gtcgccacgc
    12661 ttcggtttct ggctcatcag gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg
    12721 tcgttcattc ccagcaggca aaccactttg aacggaattg aacgcattgg catcagagta
    12781 caaatgttaa ccggtccggc gagaaaacgc tggctgatac gttcttgatc cagacgctgt
    12841 gccagttcat cacgcaatag tgacagcggc accgcgtcgc catactgcgc acctaaacct
    12901 tcggcgataa tcgcctgcca ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca
    12961 tccggcagga agaaggcgtt gagcatatcg cgacaaaccg gcaaccactc ttctagcgga
    13021 cgctcctgtg ccagcccgcg acgccagatg tttagctgca ttagcagtga agccagatgc
    13081 cccaccagtt ctgcaattaa gccgctcgat tcatcataag gtagaaccga ttgccactcg
    13141 ccctgcgcgc tctccatcgc gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg
    13201 tgttgtccgg tggcggggag ttccagctcg cgaacgttgt cgtcatctat cccccaacga
    13261 atgccggatt cgttgaccca ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac
    13321 cgcgccgcca gcaccggcac atccagcagc gccagcacat cctctgacac aaagcgactg
    13381 tcaggcagtg ataacaggct gataaacgct tccagtaccg gatgtgactg ccgcgcacga
    13441 cggtcggaaa tggcgtaagg caggtaacga tccgcaggtg cgctaccaaa cactgcctga
    13501 ataaacggac tgtagctgtc gatatcagcc accatcacga tgatgtcgcg cggagtaagt
    13561 gtcggggctt cctccagcat cgccagcaag cgatcgtgta aaacttcaac ttcacgctgc
    13621 gggctatggc aaacgtggaa tgtgatactg ctatccagtg gatcaagcgg gcgtttgtta
    13681 tcgctacggg aaaactcttc gatgttcaca cccgcaacgg cgcggttttc cagttccaga
    13741 atgtcagact gaatgttatg cagcaggtta tctggcgtca catcgacaaa agcgtccagc
    13801 tcctggctgc tctccaggtc agaaaggaga taaatgtagt cgcgcccaag cttcccccat
    13861 gaggccagca gcgagttgcc gacatcctgt tcaccatcgc tgttaaagag ctgtccggca
    13921 ttttcgctat cgcgaaatag cggcaattcg cgatcttcaa aactgtgtcg acgctgacga
    13981 gtcagcagtt tcgccagata agcaggatct ttaatatcgc cccagtaata acggcagggg
    14041 ttggtaaaca ggagatggat ttcaatatgt ttacccagcg cctggagcgc ctggagataa
    14101 acaggcggta acgcggaaat accgcatata aagacgcgcg aaggtaaccc cggcgggcag
    14161 gtcgtcgcgg actccagcgt ttcgataaag cgctgataga gattcgcgcg gtgccagcgc
    14221 ggttgtccga gttcatgggt atattccacc agcgccttcc acaacggggc ttgccaggcc
    14281 tgtgcttctc ccagcccttc aaccaaatgt cctgtttccc actgtgccag ccagtccgga
    14341 cgatagacca gatactggtc aaacaggtcc gccgcttttg aggaaagctg gaacagtttt
    14401 cgcttgtcgc tatcgtcagt cagataatgc cgcaacaggg taaagtcttc gcgctccagc
    14461 aattgcggca gcagagtcat cagtttccag ctcatgctct gtttgttaaa ggcgctctct
    14521 ttggggattt ccggtaacac ccggacgaac atatcccaga taaagctcgc tggcagcgga
    14581 aaatcaatgt ttgccgcaat accaaacttt tgcgacaggg tcatttgcag ccactgcgcc
    14641 ataccggtac tttgcaccag aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt
    14701 tcgacaataa actccatcaa cgcttccagc acgtccagac gattggaatg gtagaccctt
    14761 aacatagcgg ctcctgacta ctgacgattc gggcaatgca ggcgggtcat ctcgccttct
    14821 ctgcccccgg gtgaaactag cgtaacgctg atgctgacac atcccgcctg cgatgtctgc
    14881 attcggttga cctgccagtt ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa
    14941 ccatgccgcc agagctgttg gtactggttt ctgctggcga gactgttcat tgatgttcgc
    15001 tggatacccg ataacgcagt gacaatcatc accatcagca ccatcagcac catcgcaacc
    15061 attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat aatgccccct
    15121 ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaattttt ccatcaatga
    15181 cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aatcaataag gcttcattat
    15241 ctgccagtaa acgcaaacaa acctgggcat cggttccagc gtactgcgag cactgaactg
    15301 ctggctgcgt ctgccaggag tgcatttttc cccacgccag cgccgactga acgatggcct
    15361 ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga tcctgctgac
    15421 tcattccttg caatagcaag ctacccaaaa ccagcagcat caggaccaga gccagtgacg
    15481 aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg catccaccac
    15541 ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg gcgagaagcc
    15601 gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg gattagtgac
    15661 tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc
    15721 cttcagacga aatccaatct ggtcggactc ttttaccggt tcgcgatccc agataccgtt
    15781 actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt ccagcccttc
    15841 gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga cggtaaatac
    15901 ccgcagccag atttcatctt ccagtgccag cttacgagtg ctcgttaaac tttcacgctg
    15961 taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg ccatagcaat
    16021 caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt ttctccttgc
    16081 tgataaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc cgttgagttt
    16141 ttgaagcgaa tatgcccggc ccatgcggta ttacgcaggc caaagaaagc aagcgaaggt
    16201 gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg tgaactgcca
    16261 tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc ctccctgata
    16321 acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata gagcaaatag
    16381 tcccgcgcct agctggcggt ttgccaaagc cgttgcgact gctgccagta ttgccagcca
    16441 tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc
    16501 gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa aagacgaggg
    16561 ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag gacgttgcaa
    16621 aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac gcacactggc
    16681 gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaac gcccggatgt
    16741 ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta
    16801 cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga
    16861 tccatatggt tgctgtacag gtgcgtatcg ccaccggtcc agacaaaatc acccacttcc
    16921 agatcgcact gctgcgccat catatgcacc agtaacgcgt agctggcaat gttgaacggc
    16981 aggccgagga agacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc
    17041 acatagaact ggaagaatgc atggcacggt gccagcgcca ttttatccag ttcgcctacg
    17101 ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc
    17161 gtagtaatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca ctgtttacca
    17221 tacactggcc cgaggtcgcc attttcatcg gcccattcgt cccagatggt gacattgttt
    17281 tcgtgtagat aagcaatgtt agtatcgccc tgcaggaacc acagcagttc gtggatgatg
    17341 gaacgcaggt ggcaacgttt ggttgtcacc agcgggaatc catcctgcag gttaaaacgc
    17401 atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct
    17461 tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt
    17521 tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg
    17581 atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg
    17641 ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcct
    17701 gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcacc
    17761 acaccttcca gcagcagttc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg
    17821 tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag
    17881 cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta
    17941 ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca
    18001 gagacctgga agaaggaacg tttagtacgg cgggcgaaga taatcatcac cacgataacg
    18061 ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag atacagcgga
    18121 tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc cccgaggaag
    18181 acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca gccactgtcc
    18241 ggacgattcg cccgacgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc
    18301 aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga
    18361 aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga
    18421 acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca
    18481 aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac
    18541 cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatgacga
    18601 acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa
    18661 tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg
    18721 cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac
    18781 aaacgaagat cgataccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac
    18841 attgcaggat gaagactgtc gtaaatgttc gccacccggg tattgttgcg atcaacggcc
    18901 agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga
    18961 tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga
    19021 atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct
    19081 tcgtcaactt catcgaggct ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg
    19141 gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta
    19201 atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc
    19261 tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc
    19321 agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg
    19381 aatgggattt cagtgcgata aagaccgatg ccatcaatac agctgcccag tttttcttca
    19441 tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc
    19501 gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc
    19561 cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca
    19621 tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta
    19681 gggatcccca gcgcacgtac catgatcgca gcatgggagt tggctgagcc atctcgcacg
    19741 acaacaccga ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct
    19801 gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga
    19861 aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg
    19921 cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc
    19981 accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg
    20041 tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg
    20101 ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc
    20161 agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc
    20221 tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg
    20281 cgatattgcc caaacagggc agtcaactgc gactgagaaa gaatagctgc catctgggtg
    20341 gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt
    20401 accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg
    20461 aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca
    20521 aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc
    20581 gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt
    20641 cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg
    20701 gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt
    20761 tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat
    20821 gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc
    20881 attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa
    20941 cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcagatt gatttctgca
    21001 tcgccgctta ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc
    21061 gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca
    21121 aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc
    21181 tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca
    21241 aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg
    21301 cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat
    21361 tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca
    21421 attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt
    21481 ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc
    21541 ggagcattct gaataagccc gatatagaaa ttttaaaata ttcattatta gtcaatgcat
    21601 tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta
    21661 cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag
    21721 cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg aaaacagtag
    21781 tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta
    21841 ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc
    21901 ctaaaaagca atataaatca gagaattgaa caacacatgt ggaaaaagtt acactgcgaa
    21961 tattcggcac ataattactg tttgtttttt aaccaaggta tcatgacatg tcccaacctc
    22021 gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt
    22081 ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa
    22141 aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga
    22201 gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata
    22261 gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg
    22321 gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg
    22381 ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga
    22441 gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga
    22501 ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac
    22561 cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc
    22621 tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc
    22681 attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct
    22741 tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcttg
    22801 gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt
    22861 cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg
    22921 gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatctctt gggttacgcg
    22981 cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct
    23041 gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga
    23101 aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat
    23161 tatgctgctg gatattatct tcagcctcga ctcggtgatt accgctgtgg gtctgtcaga
    23221 tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc
    23281 gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt
    23341 cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg
    23401 ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa
    23461 caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt
    23521 ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag
    23581 actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt
    23641 tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc
    23701 aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg
    23761 gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa
    23821 attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc
    23881 cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg
    23941 ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca
    24001 cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg
    24061 tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg
    24121 aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg
    24181 aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc
    24241 gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc
    24301 gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact
    24361 gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata
    24421 cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga
    24481 acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc
    24541 gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg
    24601 cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca
    24661 cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta
    24721 gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata
    24781 tcgccagacg tcatggcctt gatcctgctc agatggcgct cgcgtttgta cgctgtcaac
    24841 cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg
    24901 aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg gtacatcagg
    24961 tttatactta tcctgcacca taaagattaa cgccggttaa aaccggcgtt aaatattaat
    25021 gacggcgctg ccagatccac agcgccgtta ttgccagcgc aaatagcgca ccgaagccaa
    25081 tgccaatggg cacgaccggg atgcctacca ttaccgccag cgagtaaatg cccagcatca
    25141 acaacatggc gctgttttcg ccaaggtttt gtactgcaat cgcattcccc gccccgacgc
    25201 tttttttacc ccgctcctgt agcaacgcat tgagcggaac gacaaaaaag ccccccagca
    25261 cgccaatcag catcaacaag gcataggctg gcagcagctc gtgttgcagg gagaaaatcg
    25321 gtaccaccac gccaatcaaa atcccggctg gcatacagcg tgacacggtt tccagcgtaa
    25381 ccagcttcgc tgccgcacct gcgccaacca cgataccaat cactaccatc gcgttgagat
    25441 aggtcggcgt agcgttatcg gtaatgccca gcgccaccgg tacccacagc accaacagga
    25501 aacgcagcgt gacacccgct ccccagaata aactggtgcc caccagcgaa aaacgcgttt
    25561 caccattgca ccatagcgag gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat
    25621 tccaggactg ccccggacgc gccgccagtt tgggaatgta gatattggca acgaccgcac
    25681 caccgtaggc cagtgcgcat gcggccagag cgaccaggac atgccagtca gccagcacac
    25741 caccggctac ggaaccgagt aaaatcgccg ctatggcaga agcttccatt aaaccgttag
    25801 cttttactaa cttactaccc gtggttaatt cgccgagaat accgtatttc gccggtgaat
    25861 aggctgcagc accaacaccc accagcgtat agccgagaaa cggattgata ccaaagcaga
    25921 tactggctgc gcccagcagc ttcaggccgt tggcaaacat catcacccgg cctttggcga
    25981 agctatccgc cacctgcccg acaaacggcg ccaaaagaat gtaagcacct acaaacacca
    26041 tttgcaggat gggctggctc cactccggat agaactgcgc tttcagtaac gtcagagtgg
    26101 caaacagtag cgcgttatcg ccaaacgcag agagaaactg cgccacgata accgctttca
    26161 tccccttcga ccacaacgaa gtgttaatgt gcactgactc actcatcgtg ttgttccgct
    26221 tcgtctaccc agcttttcaa cgtgacaaag tcaggtttgc cgctgccaag taatggcatc
    26281 tgtttcagat agcgaatatc gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca
    26341 tactgttgca acttatcgcg cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg
    26401 cctttgctgg catcgctctt aatcgcagtg gcatggactt tatctggcga aacaccaagt
    26461 gccagttgtt ccaccatttc cagcgacacc atttcgcctg caattttggc aaagcgtttt
    26521 gcgcggccct gaatctgcac aaagccctgc tcgtcaaaac gcacaatatc gccagtgtca
    26581 taccagccgc gctccatttc gccgcgaaca ttctcggcgg tgggcacttc cagtacacct
    26641 ggcttctcca cccgcagata gccgttcatt atgttcggcc ctttcagttg caggcgtccg
    26701 ccctcttcga taccagggac cgacaacagg cgtgcatcca ttcctggtag aatacgccct
    26761 accgtaccgg gtttcgccgc catcggtacg ttgatagaaa cgacaggcgc gcattcggtc
    26821 acaccgtagc cttcaaggat gcgcaggcca aatttatcct gccaaagctg tttggtactt
    26881 tcttgtagtt tttctgcgcc tgccaccaca tagcgtagac gatagaagtc atacgggttg
    26941 gcgaaacgcg cgtagtgacc gaggaaagtc gaggtaccga acaacacggt gcaactgcgg
    27001 tcatacacca agtccggcac aatgcggtaa tgcagcgggc ttggataaag gaacacttct
    27061 gcacctgtaa gcagtggcgt aaacaggcct accgtcagcc caaaggagtg aaacagcggt
    27121 aatgccgaca taaagcgatc gttggtggtg aagtcggcaa tcgttttaat ctgctcgacg
    27181 ttcgccagaa tgcttttatg gctatggacg acgccttttg gatggccttc agaaccggag
    27241 gtaaagagga tcagcgcctc ttcttccggc tgctgtttta cctgcgccag gcgcggcatc
    27301 agcaaatgag cgaagatcca tactttgtcg gcagtggtga catctgcttt taaatcttcc
    27361 agatagaccc agcgcacctg agtaagttgc tccggcagat gccagagttt gcctttatcg
    27421 agaaactggc gggaagtgaa gatggttttg atttcagccg ccgtaatagc actggtcagc
    27481 ccttttaccc cggcagtgta gttcatcatt gcgggaatgc ggcgacgcgc gatggcccca
    27541 aaaatcactg ccgcactgat gcctgcattg ggcagcatta agccgatgcg ttcgccttca
    27601 acactgtatt tttcaaggat gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag
    27661 gagtctgggg taaagttgac gtcttcgaca catttcttcc cggctccgaa gcggtacatt
    27721 gcactcagta aagattcgta cagagtttca cgcgggcgca ccgccatacg cgcttccatc
    27781 attatttgat gcagcatttc gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc
    27841 ggcatcgcca cctgcgttgg aggcaaaata tgcagagtaa tttgcgggaa caagcgacgt
    27901 ttaaccagac ctttcaggcg gctgaagtgc gtaagttccg ccccttcaat acgcacagga
    27961 ataaccgttg caccagactt cgccgcgaca aaacccgcgc catcgtagat tttcatcagc
    28021 gagcctgtag tggtgatgcg tccttcaggg aaaatcacca ctggtcggcc ctgttccacc
    28081 agacgtacca gatgtttaat agccataggt tgcgtcgggt cgagaggaac aaagtcgata
    28141 aatgatttca gccaacgcat ataccactgt tggcttattg aggtgtaaac ggcaaacact
    28201 ggacgcacag gtaaaaacag tccaagcaaa atgccatcaa taaaagagac gtgattaggc
    28261 gtaattagta cgcgctcgcc cttcagtgcc tgggtgtcac ccgtaacgcg aacgcgatac
    28321 aaaacacggc acaaatttcg aaaaaagcta aaaagcattc caactccctt tgctctgatt
    28381 cagtaaaagc gaatggaggg agattacacg agataaagaa cgcgagcgac agtaaattag
    28441 gtgcgaaaaa aaacctgcgc atccgcgcag gttggtgcaa gagacagggt acgaagagcg
    28501 taccgaataa tctcaccaat caatacctct gggatcttga ttgtggtctg cacgacgact
    28561 cttcgccagc gagaaaacgc aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc
    28621 ggttactgtt acagattgat gaccggcaaa aaaaaacctg cgcatctgcg caggctggtg
    28681 taattcatgt gctcaacacg aagttgactt cacctatcaa tacctctggg atcaccactt
    28741 tagcaacctg aagccaaacg ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa
    28801 ccaaaggttt gcaatctctt tttttgtctt acttatttgc gtttggctca cattcccacg
    28861 atgaaaacac gccacccctt gaaccaacgg gcgttttccg taacactgaa acaatgtaag
    28921 cgtttaccca ctaaggtatt ttcatggcga ccataaagga tgtagcccga ctggcaggcg
    28981 tttcagtcgc caccgtttcc cgcgtcatta ataattcacc caaagccagc gaagcttccc
    29041 ggctggctgt gcatagtgca atggagtctc ttagctatca cccgaacgcc aacgcccgtg
    29101 cgctggcgca gcagaccact gaaacggtcg gtctgatcgt tggtgatgtt tccgatccgt
    29161 ttttcggtgc aatggtgaaa gcggtcgaac aggtggctta tcacaccggt aatttcttat
    29221 tgattggcaa cggttaccac aacgaacaaa aagagcgtca ggctattgag caactgatcc
    29281 gccatcgctg tgctgcgttg gtcgtccatg ccaaaatgat cccggatgcc gatttagcct
    29341 cattaatgaa acaaatgccc ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa
    29401 accgttgtat tgctcttgac gatcgttacg gtgcctggct ggcaacgcgt catttaattc
    29461 agcaaggtca tacccgcatt ggttatctgt gctctaacca ctctatttct gacgccgaag
    29521 atcgtctgca agggtattac gatgcccttg ctgaaagtgg tattccggcc aatgatcggc
    29581 tggtgacatt tggcgaacca gacgaaagcg gcggcgaaca ggcaatgacc gagcttttgg
    29641 gacgaggaag aaatttcact gcggtagcct gttataacga ttcaatggcg gcgggcgcga
    29701 tgggcgttct caatgataat ggtattgatg taccgggtga gatttcgtta attggctttg
    29761 atgatgtgct ggtgtcacgc tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg
    29821 taacgatggc gacccaggct gccgaactgg ctttggcact ggcggataat cgccctctcc
    29881 cggaaattac taatgtattt agtccgacgc tggtacgtcg tcattcagtg tcaactccgt
    29941 cgctggaggc aagtcatcat gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc
    30001 agtaattctt cgatggtctg gcgacggcga atcaaccgcg cctgaccatt atcaaacaga
    30061 acttctggta acagcggacg gctattgtag ttggatgaca ttgatgcgcc atatgcccct
    30121 gtatcatgca gtaccagata atcacctgcc ttcacttccg gcaaggcgcg ggtttcaaca
    30181 tttccccctt cctgctgggt aaagacatcg cccgattcac ataacggccc ggcgacgacg
    30241 gtttccaccg ttggcgcgtg ttccagagaa cgaccatcag ctgccagggc actgatatgg
    30301 tggtaactac cgtacattgc cgggcgcatc agatcgttga acccggcatc aaccagcaca
    30361 aagtggcggc tacccatttg tttgacgctc cgcacctgag taattaatac gccagactgc
    30421 gctaccagga agcgacccgg ttcaatttcc agtttcacag ggtggcccaa atggcgagcg
    30481 atttgctcac gcgcggcatt ccacagacca taataatgtt cggtatcaac cgcctcttca
    30541 ccctgttgat aaggaataga aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg
    30601 aattcgataa cctgacgcac catagcacca cacacctgct ccagatgggc ataatcaacg
    30661 ccagaaccaa tgtgcatgtg aatgccgact agctgcagat gatgacgttg tatcacgtcc
    30721 agtgcggcgg gcagatcggt gtaccagata ccgtgcttgc tgttttcgcc accggtattg
    30781 gttttttggc tatgtccgtg accaaacccc ggattaacgc gcagccatac ccgatgccct
    30841 ggcgaaacct ggcccagttg gtcgagcgta tcaacagaac ccgcattcac cggaatttgc
    30901 aattcactga cgcgttcaag cgtcgcctga tcgataacat ctgccgtaaa aacaatatca
    30961 tcggggtgcg tttgcggatt gtaacccgcc gccaacgcac gctctatttc gcctaacgag
    31021 acggaatcca ctttcacgcc ctgctcacgc attaagcgca aaatatgaat attggaacag
    31081 gctttctgtg caaagcgcac cacttcaaac tgtttcagcg ctgcaatctg ccgacgaata
    31141 atttgcgcat cgtagaccca caccgggcag ccaaattcag cgggcaaacg cagcagattt
    31201 tcggcggtga gatcggtatc ggtgctgaac agtgaatgtg gcataacaaa ctccagatga
    31261 gtgctttttt atgattacgc cacatcataa aaagaataaa aaatatcgat ttatgtcgag
    31321 tctatgcaaa aatgatatgg attaccggat tgcgagagag cgctaatggc cgccgttaac
    31381 ttacgtcata ttgaaatttt tcatgcggta atgaccgccg gaagcctgac tgaggcagca
    31441 cacctgctac acacctcaca gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg
    31501 atcgggctga aattgtttga gcgcgtacgt ggacgattac atcctaccgt gcaaggactg
    31561 cgtctgtttg aagaagtgca acgatcctgg tacggactgg atcgcattgt cagcgccgca
    31621 gaaagtctgc gcgagtttcg ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg
    31681 caatcttttt taccgcagct cctgcaaccc tttctggcac gttatcccga tgtcagctta
    31741 aatatcgtgc cccaggaatc accgctactt gaagagtggc tctcggccca gcgtcatgat
    31801 ttaggactca ctgagacact ccatacgcct acgggaacag aacgtaccga attactctct
    31861 ttagatgaag tgtgtgtgtt acctccgggt catccgctgg cggtaaaaaa ggtattaacg
    31921 ccggatgatt ttcagggtga gaattacatc agcctttccc gtactgacag ctatcgccag
    31981 ttgctggatc agctatttac tgaacatcag gttaaacgac gcatgatcgt agaaacccac
    32041 agcgccgcgt cagtctgcgc aatggtacgg gcgggggtag gtatttcggt ggttaacccg
    32101 ctcaccgcac tagattatgc ggcaagcggt ttagtggtgc ggcggttcag tattgcggtt
    32161 ccgttcaccg tcagcctgat ccgccccctg caccgcccgt catcagcgct ggttcaggcg
    32221 tttagtgggc atttacaagc ggggttaccg aaactggtca cttctcttga cgctattttg
    32281 tcgtcagcta cgacagcata aaagcgacag catcctcggc atggatcgcc gcggtatcaa
    32341 acacaggcaa aacactgcgc tcttctggca ccagtaaacc aatttctgtg cagccaaaaa
    32401 tgacgccctg tgcgccctgt tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg
    32461 acgcttcggt aaattgcccc agacacagtt cttcaaaaat aatctgatta attttcgccc
    32521 gttcatccgc ttcaggaata agacaattga tggaaaattg ttccgtcagc cgcccgcgat
    32581 aaaaatcctg ttccatggtg taacgcgtac ccagcagcgc cacacgagtc attccggccc
    32641 cggtaattgc acgtccggtg gcatccgcaa tgtgtaagaa aggcagagtg caacgtgact
    32701 caatggcatc cgccacttta tgcatcgtat tggtacataa cacaatacct tctgcgcccg
    32761 cccgctgtaa gccaagcgcc gcctcagcca gaatgtcccc ggttttatcc cattccccgc
    32821 gacgctggca ctcttctatt tcatgaaaat cgacgctatg tagcagcatt tgcgcagagt
    32881 gaagcccacc aagccgctgt ttaatgcctt catttatcag acgatagtaa ggaatggtgg
    32941 attcccagct cattcctccc agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt
    33001 taccgcaaca aggctgaata aggaacgaga caaacgcctc aacggccagg tgcccaatct
    33061 ctattaacga aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc agacgccgat
    33121 atttctcaac ttctcgcctg ccatcagttt gcgttcgata tgttccagcg tgacattttt
    33181 ggtttccgga atgagccaga aagtaatacc cacaaacgca atgttcagcg cagtgtagag
    33241 ccagaacgta ccggcagcgc caatgctatc aagcagtgtc aggaaggccg cgccgataat
    33301 catattcgac acccagtttg tcgtcgtcga acaggtaata ccgaaatcgc ggcatttcag
    33361 cggctgaatt tcagagcaca ggatccacac cactggcgcg gcgctcatcg cataaccggc
    33421 aatacacatc atcgtcatgc caacagagag ccaggacaag ccactggaag ccgtaccgtt
    33481 atcaaactgc atcaggcaat agcccagcac cagagtgcct aacgccatca cgctgaaacc
    33541 aattttcaga gccggtttac gacctgcttt atctaccgta aagaccgcaa tgaaggtcgc
    33601 gaacataaag gtcagtccca cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa
    33661 gcccgccatt ttgaagatac gcggcgcgta gtacatgatg atgttcatac cggtaaactg
    33721 ctgcatcgcc tgcaacaaca taccgagaaa cacagcacga cggacgttac ggttgatctt
    33781 aaacagtgcc caaccgccct gttttaactt caggctttca cgaatttcgt tgagttcttc
    33841 tcgcgctttt tccgacgtat cgcgcagcat acgcaacact tcttccgcct caatatgacg
    33901 ccccttttcc gccagccagc gcgggctatt tggcaggaag accaccagaa taatcagcag
    33961 aactgctggt aaagcaagac cccccaacat tgcgcgccag ttaccgctat aactgaacgc
    34021 tgtatcggat aaaaacgcca gcacgatgcc gagtgtgacc atcaactggt acatactgat
    34081 catcttaccg cgaacgtttt cacttgccat ttcagaaaga tacagaggag cggtgtaaga
    34141 cgcgatcccg acagcaatgc ccagcaccac acgagcggcg attaacatct ctacgctggt
    34201 cgcaaaagcg gaccctatag aaccgagtac aaacaggatg gcccccgcca tcaggctgta
    34261 tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg ctgcgccgag
    34321 catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt gatcggtaat
    34381 gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc ctgcgaccgc
    34441 agcagctacc gaaacaaaca tattcatacg ccgcgtatca cgcaaagaac gtggcgttaa
    34501 agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa gacattgtga
    34561 aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa cagcataaat atggattaaa
    34621 ttgctgcgac atgccgttat gtgatggata ttccaatttt aaaattaagt tgaattattg
    34681 agattattat taacctccta attttaaagc agataaaatt cataaagttc attaattgat
    34741 aattgatatg gattatttca taaccatgat atggattatg atgatctaga gatataaaaa
    34801 accctgccat gcggcagggt cataaaagta agaggaatga attaacgcgc cagccaaccg
    34861 ccatccacag caatggcata accatttaca taatctgaag cgctggaggc aagaaacact
    34921 accggcccca tcaggtcact cggcagtccc caacggccag ctggaatgcg atcgagaatt
    34981 tccgcgctac gttgttcatc tgcccgcagt tgttgggtat tgttggtcgc catgtaaccc
    35041 ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgtttgccat caatcgcgtc
    35101 acacccatca cgccgctttt tgatgcggta taagaaggca cacggatccc gccctggaag
    35161 gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat aaagtgtttc
    35221 gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac atcgtcccag
    35281 tctttttcgc tgaactcgag agcgtcttcg cggcgaatca atccggcgtt attcaccagg
    35341 atatcaatat gaccaaattc agctaccgcg cgatccagca gtccaggaat gccatcaatc
    35401 tttcgcagat cggcggtcag gcttaaaaaa cgacgcccct gcgccgtgac ctgctcgatg
    35461 gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg cgccagcccc
    35521 aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac tttaccttgg
    35581 agagaaaatg cacttaaaat cataacaaca ccttattcgt ttatgcccac aactagcgca
    35641 aatctttaac gcccacatgg tccatatcat taaagacctg gttttcaccg accatgcccc
    35701 agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctc ggggagatca
    35761 ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc atcatgtgga
    35821 aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg cgctcgtggg
    35881 tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc cccatactca
    35941 attgacaggt ttccagtaca tccgggacaa aatatttgtt aatcgtgcga cggttactgg
    36001 tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt ttggtgggat
    36061 acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg gtatcaatac
    36121 tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg tgaccgattt
    36181 catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata acacctaact
    36241 cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg ccaacggaaa
    36301 ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg ctgtaaacca
    36361 tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattgcgc agcccttggg
    36421 tatccagcgt ttttgcgtgc gcaccgtgga tgctctgtct tacgtccaca tcaacctccg
    36481 ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt tttctctata
    36541 aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat gttttatttt
    36601 taatttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag aatggaaaaa
    36661 tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac agattaatga
    36721 cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc aatggtcaat
    36781 gccacgccct ggcccccgcc gatacaaagc gttgccagcc ctttgcgggc attacgtttc
    36841 accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg
    36901 agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag catcttgcca
    36961 accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac atcagccaac
    37021 tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat
    37081 gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg taaattcaac
    37141 gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt tatggatgat
    37201 gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg ggctaaacct
    37261 tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt ctgaccgtta
    37321 ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc
    37381 gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat gccatattcg
    37441 cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc ccacaaccca
    37501 tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt gcggctatca
    37561 gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac aatatcagcc
    37621 tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagaccgga accgcaaacg
    37681 tcattaatcg tgattgcaga aacgctgtta ggaagaccac ctttaatagc cgattgcctt
    37741 gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc atccaccgca
    37801 tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc aagttccacg
    37861 gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa cgccccgaca
    37921 atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta aatgattgtt
    37981 aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga aatatgcctg
    38041 agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc accaatagat
    38101 gggtagatag ggcgttaatc tcccatactt aacttggttt atggtaaatt gccctccatt
    38161 ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta aagaagaaac
    38221 tctgtgaagt ttcgcgctct atggcacagc cgttgatgca ggcacgctat tccttcctat
    38281 tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg cctggccttt
    38341 aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcaaaaa catcagcagg
    38401 tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta atctgattac
    38461 cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg caattaccaa
    38521 ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc gtatgttggt
    38581 gagtctgggt gttgtattaa ttctgaatct catttttctg atgggacgtc atgccactat
    38641 tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc tttccattta
    38701 ccttgtcggt agttggcaac ctgatctatt aacaacccag gtagagttca atcagaatac
    38761 ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc atacgcccat
    38821 tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta tggataaatg
    38881 caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt tctttgtctt
    38941 tagctgcctg ctttctattc catcttcgta tattgaagcg gctaaagaag aaggggtcac
    39001 cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa tttccgggat
    39061 tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg gcgttattga
    39121 aggtgccaca gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga aaagtcgtgc
    39181 atttaaccac gcactatcaa ttatgttggt atcgctgatt accttcattg tttgttgcat
    39241 taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg ccatgatact
    39301 tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct ggcgttccat
    39361 cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt tctttagcta
    39421 atatccgttg tggatggttc aaaattgcta caattttgaa ccattccatt tttcataata
    39481 ttcatttgtt attattccgc cctatcactt ctctcgtcat atcctagaat tatcagttag
    39541 ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc ataacttaga
    39601 ttcgcaagat atcttctggt cattcaaaaa caatttgcga tatagatatt ctcataccat
    39661 gagatagtta tctggaccac gttcgaccat gcaagattaa atcagtgaaa cataatcata
    39721 tttaataacg agaaatgctt ttttaaatat cttaatcaga attatagcaa atacagatta
    39781 atcgcaattt ttcccagcaa aattacaaaa tcaaggtgta ttgacatcat caatcaccaa
    39841 tgaaattatg caattatata cggataggga ggttcttaac atggggcttt gtagtcgtta
    39901 taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag aagagtcacc
    39961 gcgtttgcag tactgcgcaa actcgt
//
LOCUS       DADLDT020000024        39745 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000024 DADLDT020000000
VERSION     DADLDT020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39745
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.024"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..155
                     /locus_tag="JQH22_001180"
     CDS             <1..155
                     /locus_tag="JQH22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFB2205571.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="JQH22_001181"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="JQH22_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFB2205572.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3035
                     /gene="uidA"
                     /locus_tag="JQH22_001182"
     CDS             1362..3035
                     /gene="uidA"
                     /locus_tag="JQH22_001182"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFB2205573.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="JQH22_001183"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="JQH22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="JQH22_001184"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="JQH22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFB2205574.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="JQH22_001185"
     CDS             complement(5626..7134)
                     /locus_tag="JQH22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFB2205575.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JQH22_001186"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JQH22_001186"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFB2205576.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="JQH22_001187"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="JQH22_001187"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFB2205577.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="JQH22_001188"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="JQH22_001188"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFB2205578.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JQH22_001189"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JQH22_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFB2205579.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="JQH22_001190"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="JQH22_001190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFB2205580.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="JQH22_001191"
     CDS             complement(14283..15602)
                     /locus_tag="JQH22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFB2205581.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPQLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="JQH22_001192"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="JQH22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFB2205582.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="JQH22_001193"
     CDS             16699..17034
                     /locus_tag="JQH22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFB2205583.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="JQH22_001194"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="JQH22_001194"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFB2205584.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="JQH22_001195"
     CDS             complement(17790..19172)
                     /locus_tag="JQH22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2205585.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="JQH22_001196"
     CDS             complement(19358..20302)
                     /locus_tag="JQH22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFB2205586.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="JQH22_001197"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="JQH22_001197"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFB2205587.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="JQH22_001198"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="JQH22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFB2205588.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="JQH22_001199"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="JQH22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFB2205589.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="JQH22_001200"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="JQH22_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFB2205590.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="JQH22_001201"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="JQH22_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFB2205591.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="JQH22_001202"
     CDS             complement(25947..26768)
                     /locus_tag="JQH22_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFB2205592.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="JQH22_001203"
     CDS             complement(26868..26951)
                     /locus_tag="JQH22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205593.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="JQH22_001204"
     CDS             complement(27044..27379)
                     /locus_tag="JQH22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HFB2205594.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27947..28204)
                     /locus_tag="JQH22_001205"
     CDS             complement(27947..28204)
                     /locus_tag="JQH22_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205595.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28220..29029)
                     /locus_tag="JQH22_001206"
     CDS             complement(28220..29029)
                     /locus_tag="JQH22_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2205596.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29136..30038
                     /locus_tag="JQH22_001207"
     CDS             29136..30038
                     /locus_tag="JQH22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2205597.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="JQH22_001208"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="JQH22_001208"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HFB2205598.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="JQH22_001209"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="JQH22_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HFB2205599.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="JQH22_001210"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="JQH22_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFB2205600.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="JQH22_001211"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="JQH22_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFB2205601.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /locus_tag="JQH22_001212"
     CDS             complement(34274..35128)
                     /locus_tag="JQH22_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HFB2205602.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="JQH22_001213"
     CDS             complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="JQH22_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HFB2205603.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="JQH22_001214"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="JQH22_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37857..38966)
                     /locus_tag="JQH22_001215"
     CDS             complement(37857..38966)
                     /locus_tag="JQH22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFB2205604.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38949..>39745
                     /locus_tag="JQH22_001216"
     CDS             38949..>39745
                     /locus_tag="JQH22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFB2205605.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSASL"
BASE COUNT        10118 a         9635 c        10173 g         9819 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtgg
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gttgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ccagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgactagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac gctacttaag
    38941 gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt tctttctctg
    39001 ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg ctgacacaaa
    39061 tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac actgtccgct
    39121 tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc acagaccgga
    39181 ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc ctactggaca
    39241 atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct gagcagttcc
    39301 tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg gttgacgaga
    39361 agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag cagaggggca
    39421 ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact ggtgatacct
    39481 ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat aataaccata
    39541 ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag ggggatagac
    39601 cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa tcatctgtac
    39661 tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg aaacaatcca
    39721 gctttaagga cttgtcagcg tccct
//
LOCUS       DADLDT020000025        38862 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000025 DADLDT020000000
VERSION     DADLDT020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38862
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.025"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1546
                     /locus_tag="JQH22_001217"
     CDS             <1..1546
                     /locus_tag="JQH22_001217"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFB2205606.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
     gene            1705..2264
                     /locus_tag="JQH22_001218"
                     /pseudo
     CDS             1705..2264
                     /locus_tag="JQH22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2265..3914
                     /locus_tag="JQH22_001219"
     CDS             2265..3914
                     /locus_tag="JQH22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFB2205607.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="JQH22_001220"
     CDS             3919..4695
                     /locus_tag="JQH22_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFB2205608.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="JQH22_001221"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="JQH22_001221"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFB2205609.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JQH22_001222"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JQH22_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HFB2205610.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JQH22_001223"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JQH22_001223"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFB2205611.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="JQH22_001224"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="JQH22_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFB2205612.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="JQH22_001225"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="JQH22_001225"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFB2205613.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="JQH22_001226"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="JQH22_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFB2205614.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="JQH22_001227"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="JQH22_001227"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFB2205615.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="JQH22_001228"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="JQH22_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFB2205616.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18109..18468
                     /locus_tag="JQH22_001229"
                     /pseudo
     CDS             18109..18468
                     /locus_tag="JQH22_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18702..19721
                     /locus_tag="JQH22_001230"
     CDS             18702..19721
                     /locus_tag="JQH22_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFB2205617.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(19871..20359)
                     /gene="eco"
                     /locus_tag="JQH22_001231"
     CDS             complement(19871..20359)
                     /gene="eco"
                     /locus_tag="JQH22_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFB2205618.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20494..20658)
                     /locus_tag="JQH22_001232"
     CDS             complement(20494..20658)
                     /locus_tag="JQH22_001232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205619.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            20767..21261
                     /gene="napF"
                     /locus_tag="JQH22_001233"
     CDS             20767..21261
                     /gene="napF"
                     /locus_tag="JQH22_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFB2205620.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21251..21514
                     /gene="napD"
                     /locus_tag="JQH22_001234"
     CDS             21251..21514
                     /gene="napD"
                     /locus_tag="JQH22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFB2205621.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21511..23997
                     /gene="napA"
                     /locus_tag="JQH22_001235"
     CDS             21511..23997
                     /gene="napA"
                     /locus_tag="JQH22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFB2205622.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELVEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            24004..24699
                     /gene="napG"
                     /locus_tag="JQH22_001236"
     CDS             24004..24699
                     /gene="napG"
                     /locus_tag="JQH22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFB2205623.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24686..25549
                     /gene="napH"
                     /locus_tag="JQH22_001237"
     CDS             24686..25549
                     /gene="napH"
                     /locus_tag="JQH22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFB2205624.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25546..25995
                     /gene="napB"
                     /locus_tag="JQH22_001238"
     CDS             25546..25995
                     /gene="napB"
                     /locus_tag="JQH22_001238"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFB2205625.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            26005..26607
                     /gene="napC"
                     /locus_tag="JQH22_001239"
     CDS             26005..26607
                     /gene="napC"
                     /locus_tag="JQH22_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFB2205626.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26626..27243
                     /gene="ccmA"
                     /locus_tag="JQH22_001240"
     CDS             26626..27243
                     /gene="ccmA"
                     /locus_tag="JQH22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFB2205627.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27240..27902
                     /gene="ccmB"
                     /locus_tag="JQH22_001241"
     CDS             27240..27902
                     /gene="ccmB"
                     /locus_tag="JQH22_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFB2205628.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27944..28681
                     /gene="ccmC"
                     /locus_tag="JQH22_001242"
     CDS             27944..28681
                     /gene="ccmC"
                     /locus_tag="JQH22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFB2205629.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28678..28887
                     /gene="ccmD"
                     /locus_tag="JQH22_001243"
     CDS             28678..28887
                     /gene="ccmD"
                     /locus_tag="JQH22_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFB2205630.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28884..29363
                     /gene="ccmE"
                     /locus_tag="JQH22_001244"
     CDS             28884..29363
                     /gene="ccmE"
                     /locus_tag="JQH22_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFB2205631.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29360..31303
                     /gene="ccmF"
                     /locus_tag="JQH22_001245"
     CDS             29360..31303
                     /gene="ccmF"
                     /locus_tag="JQH22_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFB2205632.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31300..31857
                     /gene="dsbE"
                     /locus_tag="JQH22_001246"
     CDS             31300..31857
                     /gene="dsbE"
                     /locus_tag="JQH22_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFB2205633.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            31854..32906
                     /gene="ccmH"
                     /locus_tag="JQH22_001247"
     CDS             31854..32906
                     /gene="ccmH"
                     /locus_tag="JQH22_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFB2205634.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33117..33764)
                     /gene="narP"
                     /locus_tag="JQH22_001248"
     CDS             complement(33117..33764)
                     /gene="narP"
                     /locus_tag="JQH22_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFB2205635.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34084..36675
                     /gene="yejO"
                     /locus_tag="JQH22_001249"
     CDS             34084..36675
                     /gene="yejO"
                     /locus_tag="JQH22_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFB2205636.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(38085..38363)
                     /locus_tag="JQH22_001250"
     CDS             complement(38085..38363)
                     /locus_tag="JQH22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFB2205637.1"
                     /translation="MIHDSKAEDLESKGLYRRAAARWAEVMQQVNTDKEREQAVKRRA
                     ECIHKAARSPVILDNFGGLREAANRTQADMGIEIAKSVWRNYQTDKVL"
     gene            complement(38366..>38862)
                     /locus_tag="JQH22_001251"
     CDS             complement(38366..>38862)
                     /locus_tag="JQH22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HFB2205638.1"
                     /translation="YGIPKKKTQTTQQDKTTSAHKVIPGNQNAPQSRQQKPTGATPWR
                     HMTKRQGKNRRRANRLIELWPELFSLESPKPLKVGIFEDLIQDIAVRAQAFGSGALRA
                     ALASYAHSPRYYRALMAGGVRYDLNSQPCGEVTQQEQQNAEARLMALKEKRKRKSNKE
                     QAGV"
BASE COUNT         9067 a         9459 c        10745 g         9591 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctctagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagccga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttaccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggtaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tattgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgagtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgccatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg agcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
    17941 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
    18001 tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg
    18061 cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg
    18121 actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg
    18181 aaagcgtccg aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc
    18241 acgttgggga cctattgacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc
    18301 ctggaggggg gcgcgcagga gagttcgaac ggctagaaca acgccggaac cgggcattct
    18361 gcactgatgg aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa
    18421 gcagtcgcca ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggtcgag
    18481 cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc
    18541 tggggcgagg ataacgttaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg
    18601 ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta
    18661 gtgatggaag ggcgcgatcc gcaacagaaa ttggcactga tgtgaactat ggtgagatca
    18721 cccgccagtt aattgcttcc ttacagaaga aatctaactt ctcgctgcaa ctcagcagcg
    18781 aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt gccgatctga
    18841 aaaacggcac tgcacagaac atccgcgcca aatttgtctt tatcggcgcg ggcggtgcgg
    18901 cgctgaagct gttacaggaa tcggggattc cggaagcgaa agactacgcc ggtttcccgg
    18961 tgggcggaca gttccttgtt tcggaaaatc cagacgtggt taatcgccat ctggcgaaag
    19021 tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc gatacccgtg
    19081 ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca accaaattcc
    19141 tcaaaaacgg ttcattgtgg gatctgatga gttccaccac cacctctaac gtgatgccga
    19201 tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt caggtgatgt
    19261 tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaagcg aaaaaagagg
    19321 actggcgttt gtggcaagcg gggcagcgtg tgcagattat caagcgtgat gccgagaaag
    19381 gtggcgtact gcgtctgggt accgaagtgg tcagtgacca gcaagggacc attgcggcgc
    19441 tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttgaat ctactggaaa
    19501 aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc
    19561 cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag ttgcagtaca
    19621 ccagcgaagt gctggggctg aaatacgaca aaccgcaagc agcagatagt acgccgaaac
    19681 cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc acatccggca
    19741 tggtatgccg gatgtggcgt atactgataa gacgcgccag cgtcgcatca ggcaaccggc
    19801 tcgggcgtta gatgttggat gcggcgtaac cgccttatcc aaccaacggg gtgccgtact
    19861 tcacaacagt ttagcgaact tccgcgttgt caattttctc ttccgccttc cagacgcggt
    19921 atttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca
    19981 gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc
    20041 aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa gacataataa tcatagcccc
    20101 agccttccag cgttttgctt tccagcttcc cgccgagacg atgcaaattg caatcgactt
    20161 ccagcgtctg accgattaac agttctactt tcagggtaga ttcatcttct tgcggggtta
    20221 actgaatcac ctgacgcttc atgcctgttt cagcttgtgg ataaggcgcg attttttcca
    20281 gtggctggac gctttctgcc gcccaggcgg aagtggtagc gaaagcggca aacaatactt
    20341 caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga
    20401 tgtcgctgaa ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag
    20461 aacattcaga caattttacc tgctctttta gtgttaaatt ctgatgaggg agcaaaccag
    20521 cagaaatatc tctggtgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg
    20581 agcaaaagcc cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac
    20641 cccgatcggg gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc
    20701 ttattaatta tgcggtctat ttggtcgctc tcaattttca gagcgcgtta atgatggaag
    20761 gtcaatgtga agattgatgc atcccgtcgg ggcatactca ctggttgctg gcgcaaagcc
    20821 agtaacggta tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc
    20881 cgctgcgacg cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cacgggtggc
    20941 tatccgagcg ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct
    21001 tgccccgaat cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatc
    21061 ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag
    21121 ccaatggcaa ttatctttcg cccgacgctc tccgggatct accaggcgca acttaatagc
    21181 caactttgca acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg
    21241 gagtatctcc atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga
    21301 acgaatttca gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag
    21361 cgacgcgccg agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat
    21421 ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca
    21481 ccagcaggaa gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa
    21541 gctaacgccg ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc
    21601 gccgttgttg gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt
    21661 actggttgcg gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac
    21721 ccggacgcac cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc
    21781 atgtacggta aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac
    21841 aaagaaggcg aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa
    21901 ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag
    21961 tggactatct gggaaggtta tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac
    22021 aacatcgacc cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc
    22081 tttggtatgg atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg
    22141 ctgtggggcg ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaacagt
    22201 cgtctctcta accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc
    22261 gagctggcgg ataacggcat catctttacg ccgcaatctg acctggtgat cctgaactac
    22321 atcgccaact atatcattca aaacaatgcg ataaatcagg acttcttcag caagcacgtt
    22381 aacctgcgca aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa
    22441 aaagcagcga agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa
    22501 gccttcgttg ctgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac
    22561 cagttagaac aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg
    22621 acgatgggct tcaaccagca tacccgtggt gtgtgggcta acaacctggt ctacaacctg
    22681 cacctgctga ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcaa
    22741 ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac
    22801 atggtggtaa ctaacgagaa acatcgtgat atctgtgaga agaagtggaa tatcccgagc
    22861 ggcaccattc cggcgaaaat cggtcttcat gcggtggcac aagaccgtgc gctgaaagac
    22921 ggcaagctga atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc
    22981 aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg
    23041 tatccgacag tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag
    23101 aaagagggcg cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag
    23161 gcgccaggcg aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa
    23221 actgaagaag tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg
    23281 ctgtacgaag ttctgtatgc cacccccgaa gtgagcaaat tcccggtatc cgaactggtg
    23341 gaagatcagc tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc
    23401 gaagagtacg catggtttgg tcgcggtcac ggtcacgatc tggcaccgtt cgatgactac
    23461 cacaaagcgc gcggtctgcg ctggccggtg gtgaatggta aagaaacaca gtggcgttac
    23521 agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg
    23581 gatggcaaag cggtgatctt cgcactgccg ttcgaaccgg cggcggaagc accggatgaa
    23641 gagtacgacc tgtggctctc taccggacgc gttctggagc actggcacac cggcagtatg
    23701 actcgccgtg tgccggaact gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg
    23761 ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt
    23821 ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg
    23881 tacatgccgt tcttcgacgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat
    23941 ccgctctcga aagagacgga cttcaagaag tgcgcggtca aactggagaa ggtgtaagcc
    24001 attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt
    24061 cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca
    24121 cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt
    24181 gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg
    24241 acgctggcct ctggtctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc
    24301 gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag
    24361 attgaatcga tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt
    24421 ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag
    24481 gccatcacct tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg
    24541 acggttcaca gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa
    24601 caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac
    24661 cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc
    24721 gctggagaaa aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca
    24781 gttcttcgtg ctggggatgt ttttgagcgg tccatggttt ggtgtgtgga tcttgcacgg
    24841 caactacagc agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct
    24901 gcaaagtctt gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat
    24961 caccgtgctc tacgccctgg cgggaaagcg attattttgc agctgggtct gcccgctgaa
    25021 cccgattacc gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac
    25081 gatccctcgc cacattcgct acgtgctgtt ggtggtcatt ctggtgggat cagccttaac
    25141 tggcacgctc atttgggaat ggatcaaccc cgtttctctg atggggcgta gcctggtaat
    25201 gggcttcggc agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt
    25261 tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag
    25321 caaaggtgtg attaccgttg cggccagcga tcggcagaaa tgtaaccgct gtatggattg
    25381 ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc
    25441 ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga
    25501 ggatgtattt acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac
    25561 ctgaagaaag cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt
    25621 tgggcggcta atggcgtcga ttttagccag tcgccggaag tatccgggac gcaggaaggg
    25681 gccattcgga tgccaaaaga gcaggatcgg atgccgctga actatgtgaa tcagccgcca
    25741 atgatcccgc atagcgttga aggttatcag gtaacgacca ataccaatcg ctgcttgcag
    25801 tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt
    25861 atggacagcg acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa
    25921 tgtcacgtac cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa
    25981 ggttacggga aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag
    26041 cgcctgtgga aatggtggcg tacccccagc catctggcgc tggggacgct gctgttgatc
    26101 ggttttgttg gcggcatcgt cttctggggg ggctttaaca ccgggatgga aaaagccaat
    26161 accgaagagt tctgcattag ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg
    26221 gattccgtgc actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt
    26281 ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt
    26341 aaaatttttg gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca
    26401 cagaatgagt ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac
    26461 ttcgagtata tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg
    26521 gtgaaagatg ggcaaacctg tattgattgc cataaaggga tcgcgcacaa gctgcccgat
    26581 atgcgtgaag tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga
    26641 gagttacttt gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac
    26701 gcaggagagt gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt
    26761 ttgctgacgg ggttgtctcg ccctgacgct ggcgaggttc tctggcaagg gcagcccttg
    26821 catcaggtac gcgacagcta ccatcaaacc ctgttatgga taggccatca gccggggatc
    26881 aaaacccggc tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgatgccgca
    26941 caatgtctgg aagccttggc gcaggccggg cttgccggat tcgaagatat tccggtaaat
    27001 caactttctg ccgggcaaca acgccgcgtc gctttagcgc gtctgtggtt gacccgtgcc
    27061 acattatgga tcctcgacga gccttttacc acgattgacg ttaacggcgt cgatcgtctg
    27121 acccagcgta tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag
    27181 ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca
    27241 tgatgttctg gcgtattttc cgtcttgaac tgcgcgtagc gtttcgccat agcgccgaaa
    27301 tcgccaaccc gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc
    27361 cggagccgca actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt
    27421 catccttgct ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac
    27481 aattgatgtt gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact
    27541 ggatggtaac cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa
    27601 tggatgttta tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg
    27661 gctttctcgg tgcaccgggc gtggcgctga cagtgggact taagcgcggt ggtgtgctgc
    27721 tcagcatact ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg
    27781 acgcggcttc tatgcatttg cccgttgatg ggtatctggc aattttaggc gcgttgctgg
    27841 caggcaccgc gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat
    27901 aacgctggat tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca
    27961 tcaactggcg atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc
    28021 aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc
    28081 tgattatcag cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg
    28141 gtcgatgggc atttatgcat caatggcggt ggcggcgttt attggccttg tctggcagat
    28201 gaaaatggcc aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat
    28261 tgccctggtt accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga
    28321 tgcacgtctg acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg
    28381 gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg
    28441 cgtggtgaat ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg
    28501 atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat
    28561 ttttggcttc ctgctcctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt
    28621 gctgatggaa aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg
    28681 acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc
    28741 tggctggcgg tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg
    28801 caacatcgcg caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt
    28861 gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct
    28921 gtgccgtgtt ggcagggcta gcgctgacta tcggtctggt gctatatgcg ctgcgctcga
    28981 atatcgatct cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaga
    29041 tgccggaagt cggtcagcgt ctgcgcgttg gcgggatggt gatgccgggt agcgtgcagc
    29101 gcgatcccaa ttcgctgaaa gtgaccttca ccatttacga tgctgaaggc tcagtggatg
    29161 tctcttacga aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg
    29221 gcgagctgga aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa
    29281 actacacgcc gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg
    29341 tttataagga cccagcatca tgatgccgga aattggaaac gggctgctgt gcttggcgtt
    29401 agggattgcg ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg
    29461 catgatggcg tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt
    29521 tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa
    29581 ctccaatacc cagcttccgg tgtggtatcg cgtggcggct acttggggcg cgcatgaagg
    29641 ttcgcttctg ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag
    29701 tcagcgtatt ccgctggata ttgtggcccg tgtactggcg ataatgggga tggtcagcgt
    29761 cggctttttg ctgttcattc tctttacctc taacccgttc tcacgcacgc tgccgaactt
    29821 cccgattgaa gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc
    29881 gcctctgctt tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc
    29941 tttgctgagc gggcgtctgg acagcactta tgcgcgtttt actcgcccgt ggacgctggc
    30001 ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact
    30061 cggctggggt ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct
    30121 ggtggggact gcactgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc
    30181 gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt
    30241 gcgttccggc gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt
    30301 tatcctcgcc tttatggtgc tggtgattgg cggttcgctg ctgctgtttg ccgcgcgtgg
    30361 acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg cgggaatctt tgctgttagc
    30421 gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg ctggggacgc tgctgccgct
    30481 ggtgcacaag caacttggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat
    30541 gtttacctgg ctgatggtgc cgtttgcgct actgcttggt gtcggtcctc tggtgcgctg
    30601 ggggcgcgat cgcccacgta aaatccgcaa tttattgatt atcgccttca tctctacgct
    30661 ggtgctgtct ctgctgttgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct
    30721 cggcctggca atggcctgct ggattgcggt gctggcaact gcggaagctg cgctgcgtat
    30781 ttcacgcggc acgaaaacca ccttcagtta ttgggggatg gtggcggctc acctggggct
    30841 ggcagtgaca attgttggca ttgcctttag ccagaattat agcgttgagc gtgatgtgcg
    30901 catgaagtcc ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa
    30961 agaggtgact ggcccgaact ggcgcggcgg tgtggcgact attggcgtaa cgcgagaggg
    31021 taaaccggaa acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat
    31081 gaccgaagcg gcaattgacg gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga
    31141 gctggaaaac ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg
    31201 ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg
    31261 taagcgcgtg agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta
    31321 attccgttga ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat
    31381 gccgaagggg atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag
    31441 tttcgtctcg aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag
    31501 ggcaaaccag tactgcttaa cgtctgggcg acttggtgtc cgacctgccg tgcggaacat
    31561 caatatctga atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac
    31621 gatcgccaga aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg
    31681 tttgatggcg acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaaccttc
    31741 cttattgacg gcaacggtat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc
    31801 tgggaagaaa agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt
    31861 ttttattggg cgtgctgatg ctgatgatct ccggctcagc gctggcgacc atcgacgtgt
    31921 tgcagtttaa agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct
    31981 gcccgaaatg ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc
    32041 gtcagaaagt gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata
    32101 tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc
    32161 tgctgtgggt gctgccagta gtggctattg gcattggcgg ttgggtcata tacgcccgct
    32221 cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga acaaagcgtg ccggaaggta
    32281 agcgtgccgg atatgttgtt tatctgccgg gtattgtggt ggcgttaatt gtggctggcg
    32341 tcagctacta ccagactggc aattatcagc aggtgaaaat ctggcagcag gccacggcac
    32401 aggctccggc gttgctggac agggcgctgg acccgaaagc cgatccgctc aacgaagaag
    32461 agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag
    32521 aaggctggat tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg
    32581 atgcatacgc tactgcgtat cgcctcgatc cgaagaacag cgatgccgca ctgggatacg
    32641 ctgaagcgtt gactcgttca tctgatccca acgacaaccg cctcggcggt gaactgctac
    32701 gtcagctggt gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg
    32761 cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct
    32821 tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac
    32881 atttgtcgcc gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctagac
    32941 tgatgacaaa gtgcgcttag tttatgccgg atgcggcgtg aacgccttat ccggcctaca
    33001 aaattgtgta aattcaataa attgatcgat ccacgtaggc ctgataagcg tagcgcatca
    33061 ggcagttttg cgtttgtcat aaatctcagc ccgatgataa atccatcggg ctatttttat
    33121 tgtgccccgc gttgttgcag gaacagaatg gtggccgcca cgcgtgagcg gacattgagt
    33181 ttacgcagca gattgcgaat atgtactttt actgtctgct cggaaatatt caacaccgat
    33241 gcaatctgtt tatttgacag cccctgtgcc agctcgtgca gaacatccag ttcgcgctcc
    33301 gtcagcacgc tgaagggatc ttcttccgcg ccaaacattt cacgttcacg taagtactga
    33361 ttgacgcgtt cgctaaagac tttgctgcct ttcgctccgg tacgaatcgc ttccagcaat
    33421 acttccgggt cgctgtcttt caacagataa ccgtctgcgc ctgcgtctat cagcgcaaag
    33481 acatcgctgg aggcatcgga tacggtcagg ataataattt gcgcggtaac gccatccctg
    33541 cgcaaggcat tgagggtatc caggccactc atacctttca tattgagatc cagcaagatc
    33601 acgtcgatat ccagtctatt cgccagatcg atcgcgctcg cgccttcgcc cgcttcggcg
    33661 accacttcaa agccaggatc aagctccagt aactgacgaa caccgcgtcg cataagtgga
    33721 tgatcatcca caatcatcac ctgaaaaggc gttgcttcag gcatagtagt ctcctgaggt
    33781 tttattagaa tcattatgtt tgagcgtagc gttggtaacg ctaccaggcg cgtgaatgtt
    33841 accccatttt atgtatggac atgtagtgaa aactacaacc ttgtcgttcg ctttggcatc
    33901 atttttatta taaagacgga attttgcatc agcttctcat caagaaaaac aactaaaaaa
    33961 tcaatcacaa tacttgtgca tttctttaaa cagtttttaa aaactgactc cgggtatgga
    34021 gctatgggta ttttctgtac ccaatgcttt taactgcaat taatttcata ggatgaaagc
    34081 tcaatgcatc aatctggttc tgtttctctt tgtcgttccg caatatctgt tctggtggct
    34141 acagcgttat attcacccat agcattggca tcaacagttg agtatggtga gacagttgat
    34201 ggtgttatcc tggaaaaaga tatccagctg gtttatggga ccgccaataa tacgaaaatc
    34261 aatcctggcg gagaacagca tataaaagaa tttggtgtaa gtagtaatac tgaaattaac
    34321 ggagggtatc aatacattga aatgaatggt accgcagaat actcagtatt aaatgatggt
    34381 tatcaaattg ttcaaatggg tggcgcggca aaccagacta ctctcaataa tggtgtgcta
    34441 caggtttatg gcccagcgaa tgataccacg attaaaggcg ggcgcttaat cgttgaaaaa
    34501 gatgggggga ccgtttttgc cgctatcgaa aagggaggac tactggaggt taaagagggg
    34561 ggatttgcgc ttgcggtaga tcagaaagca ggcggtgcta ttaaaacaac cacgcgggcc
    34621 atggaggtat tcggaacaaa ccgtctcggt cagttcgata tcaagaatgg tattgctaat
    34681 aatatgttgt tggaaaacgg cggaagtttg cgagttgaag aaaatgactt cgcttataat
    34741 actactgtgg atagtggcgg cttactggag gttatggatg gcgggactgc aactggcgtt
    34801 gataaaaaag caggcgggaa attaattgtc tcaacgaatg cgctggaagt gagtggtaca
    34861 aacagtaaag gccaatttag tataaaggat ggtgtgtcaa aaaattatga actggatgat
    34921 ggttccgggc tcattgttat ggaggacacg caggccattg acactatcct tgatgagcat
    34981 gccactatgc aatcgctggg aaaggatact ggtacgaaag tgcaggcaaa tgcggtatat
    35041 gatctcggtc gatcagacca gaatggaagt atcacgtatt cctcaaaagc catctctgaa
    35101 aatatggtaa tcaacaatgg ccgcgctaat gtctgggctg gcacaatggt taacgtgtca
    35161 gtcagaggga atgatggcat tcttgaggtc atgaagccgc aaataaatta tgcacccgct
    35221 atgttggtgg gtaaggtagt ggtttctgag ggcgcttctt ttagaacgca tggtgccgtg
    35281 gataccagca aagcggacgt ttcgctcgaa aatagcgcat ggaccatcat tgccgatatc
    35341 actacgacga accaaaacac tcgcctcaac ttagccaacc ttgcgatgtc cggcgcaaat
    35401 gtgattatga tggctgagcc agtgactcgt tcatctgtga cggcaagtgc ggaaaatttc
    35461 attacgttga ccaccaatac cctgtcggga aacggcaatt tttatatgcg taccgatatg
    35521 gccaatcatc agagcgatca gctcaacgtc accggtcagg caacaggtga tttcaaaata
    35581 ttcgtgacgg acaccggtgc cagcccggca gcaggagata gccttacact ggtaacaacg
    35641 ggcggcggtg atgctgcatt tacgttgggc aatgccggag gcgttgttga tatcggtacg
    35701 tatgaatata ccttgctgga taatggcaac catagctgga gcctcgcaga gaatcgcgcg
    35761 caaattaccc cttcaaccac tgatgtgctg aacatggcag ccgcacaacc gctggtattt
    35821 gatgcagaac tggacaccgt gcgtgagcgt cttggtagcg taaaaggcgt tagttacgat
    35881 acggtgatgt ggagttcggc aattaacacc cgcaacaacg tgaccactga tgcgggagct
    35941 ggttttgagc aaacattgac gggcctgacg ctcggtatcg atagccgttt ctcccgtgaa
    36001 gaaagcagta caattcgcgg cttgttcttt ggttactctc attctgatat tggttttgat
    36061 cgcggcggaa aaggtaatat cgatagctat accctggggg cttatgccgg ttgggagcat
    36121 cagaacggtg cctatgttga tggggtggtg aaagttgacc gttttgccaa caccatccat
    36181 ggcaagatga gtaatggggc aacagcgttt ggcgattaca atagtaacgg cgcgggtgct
    36241 catgttgaga gcgggttccg ttgggttgac ggattgtgga gtgttagacc ctatctggcc
    36301 tttaccggct ttaccacaga tggtcaggac tacacgttat caaacggcat gcgcgcggat
    36361 gtgggaaata cccggatatt acgcgctgaa gcgggaacgg cggtaagcta tcacatggac
    36421 ctgcaaaacg gtacgacgct ggaaccctgg ctgaaagccg ccgtgcgtca ggaatacgcc
    36481 gattctaacc aggtgaaagt taatgacgat ggcaaattta ataatgatgt ggctggaacc
    36541 agtggcgttt atcaggctgg gataaggtca tcgtttaccc cgacgttaag cggtcatttg
    36601 tcagtcagct atggcaatgg cgcaggggta gaatcgccgt ggaatactca ggcgggtgtg
    36661 gtctggacgt tctgataaca gaaaataaac aggctgtgat gtgtcacggt ctgtttatcg
    36721 aattaattgc agatataaaa aaaccaaccg taagggttgg ttttttcttg ggatttttgg
    36781 cacgagagta gtttaattga gcctgtaact gcatgatttt aaatatgtag attaattgag
    36841 ttttgaaatc gtatatccaa acgtataact aaagtgattt gctgcaaaaa tgtacagctt
    36901 taaccgttat cattttccgg ctatccgcta tgaagctggc tggtggatga tcacggataa
    36961 cctgacatct acagccgcct taaaaagctg aacccgacaa gcatatcggc atcatgtcca
    37021 ccagcgtgac gcattttttt aattgcgcgg gcgtaaaaat ttgacggggc ggacagttcc
    37081 ataagtaatg gattgcagga atttagcccc cctcctgaat gcaattttcg tatatatata
    37141 tacaaaacta cctgtcccac ctgtcccacc tgtcccacct gtcccactag cacttaatag
    37201 attgattttg ttagaaatga attaggtaag gtgggacaag tccaggctgt tttgggacaa
    37261 gttgggggga agtgggacaa gtcgagcaag tgaatcaagc cgtgttagcg cggtaaaccg
    37321 actgtgctgc cgcaattttg cgttgtcggg agggtactgt aacagccacc atacgcgcac
    37381 aatcttcggt ggtaacgcaa tccagacgtg cgcaacatcc ccagacaggt aaatcattac
    37441 ctagcacagc taattgcgcc agaatcgatt acagcgcgtt tctgtggcat tcaagaacgg
    37501 ctatcactag tgtattgata tgtaatccgc atatgagttt aagcaacaaa atgatccctt
    37561 ttcgaaaaag gcactaaccg ttttttccgc aatgatccgt tggtgatttt gtaacttatt
    37621 gttttgtatg tatgatttta aatttggtag ggttataaat aggggatggt tttgtcaaga
    37681 attgcaaact atgcttattt attgctcttg gttcgtttgt tttctgttcg ttaatggggg
    37741 atcatttttc actttttggg ggatcatttc ggagggggtg gggctggcaa cacgccagca
    37801 ccctcgatgc gtttgtgttt agcagtactc ccagtctgtg gctagtagac tctcaggaga
    37861 tagcgtaacg ctatcctctg tgacgggatt caacgcaatc aaatcgtttc cgcgccgttc
    37921 gagaaagacg atgtacggaa gatgccaggc actgcggcga gctggcacgc gtcggcctgg
    37981 ctgcaacttg tcgataatat ccaccagcga aacgttcatg tgattcaaaa aactcctgaa
    38041 acgctctaca actcaataac acaaaaaata atgcagaact gccactataa aaccttgtct
    38101 gtctggtagt tccgccagac agattttgcg atttctatcc ccatatccgc ctgtgtgcgg
    38161 tttgctgcct ctctcaagcc cccgaaatta tccagtatga cgggcgaacg tgctgccttg
    38221 tggatacatt ccgcgcgacg ttttactgcc tgttcgcgct ccttatcggt gttcacctgt
    38281 tgcatgacct cagcccaccg ggccgccgct ctccggtaca ggcctttaga ctccagatct
    38341 tccgctttac tgtcgtgaat catactcata cgccagcttg ctctttgttc gacttacgtt
    38401 tgcgcttctc tttcagtgcc atcagtcgtg cttccgcgtt ctgctgttcc tgttgtgtca
    38461 cctcgccgca tggctggcta ttcagatcgt aacgcacgcc accagccatt aaggcacggt
    38521 aataacgcgg agaatgagca taagatgcta gcgctgcacg taatgcccct gacccaaaag
    38581 cctgcgccct gacggcaata tcctgtatca ggtcttcaaa tatccctacc ttaagcggct
    38641 tcggtgattc caggctgaat aattcaggcc atagttcaat aagacggtta gcgcgcctgc
    38701 ggtttttgcc ctggcgtttg gtcatatgcc gccatggtgt cgcccctgtg ggcttctgtt
    38761 ggcggctctg cggtgcgttc tgattgccgg gtatcacttt atgcgctgat gtggttttat
    38821 cctgctgtgt cgtctgtgtc tttttctttg gtatgccgta aa
//
LOCUS       DADLDT020000026        38775 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000026 DADLDT020000000
VERSION     DADLDT020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.026"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JQH22_001252"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JQH22_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFB2205639.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="JQH22_001253"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="JQH22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFB2205640.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="JQH22_001254"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="JQH22_001254"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFB2205641.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="JQH22_001255"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="JQH22_001255"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFB2205642.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="JQH22_001256"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="JQH22_001256"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFB2205643.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="JQH22_001257"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="JQH22_001257"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFB2205644.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="JQH22_001258"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="JQH22_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFB2205645.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="JQH22_001259"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="JQH22_001259"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFB2205646.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="JQH22_001260"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="JQH22_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFB2205647.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="JQH22_001261"
     CDS             7138..7749
                     /locus_tag="JQH22_001261"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFB2205648.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="JQH22_001262"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="JQH22_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFB2205649.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="JQH22_001263"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="JQH22_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="JQH22_001264"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="JQH22_001264"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFB2205650.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="JQH22_001265"
     CDS             complement(11943..12302)
                     /locus_tag="JQH22_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205651.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="JQH22_001266"
     CDS             complement(12299..12805)
                     /locus_tag="JQH22_001266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205652.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="JQH22_001267"
     CDS             complement(12951..13514)
                     /locus_tag="JQH22_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205653.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="JQH22_001268"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="JQH22_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="JQH22_001269"
     CDS             complement(14722..15096)
                     /locus_tag="JQH22_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HFB2205654.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="JQH22_001270"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="JQH22_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HFB2205655.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="JQH22_001271"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="JQH22_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFB2205656.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="JQH22_001272"
     CDS             complement(17166..18056)
                     /locus_tag="JQH22_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFB2205657.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="JQH22_001273"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="JQH22_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFB2205658.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="JQH22_001274"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="JQH22_001274"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFB2205659.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="JQH22_001275"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="JQH22_001275"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFB2205660.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="JQH22_001276"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="JQH22_001276"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFB2205661.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="JQH22_001277"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="JQH22_001277"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFB2205662.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="JQH22_001278"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="JQH22_001278"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFB2205663.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="JQH22_001279"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="JQH22_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFB2205664.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="JQH22_001280"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="JQH22_001280"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFB2205665.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="JQH22_001281"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="JQH22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFB2205666.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="JQH22_001282"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="JQH22_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFB2205667.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="JQH22_001283"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="JQH22_001283"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFB2205668.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="JQH22_001284"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="JQH22_001284"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFB2205669.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="JQH22_001285"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="JQH22_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFB2205670.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="JQH22_001286"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="JQH22_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFB2205671.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="JQH22_001287"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="JQH22_001287"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFB2205672.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="JQH22_001288"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="JQH22_001288"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="JQH22_001289"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="JQH22_001289"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFB2205673.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="JQH22_001290"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="JQH22_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFB2205674.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="JQH22_001291"
     CDS             complement(38654..>38775)
                     /locus_tag="JQH22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFB2205675.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9708 c         8723 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DADLDT020000027        37628 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000027 DADLDT020000000
VERSION     DADLDT020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.027"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..175)
                     /locus_tag="JQH22_001292"
     CDS             complement(<1..175)
                     /locus_tag="JQH22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFB2205676.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JQH22_001293"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JQH22_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFB2205677.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JQH22_001294"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JQH22_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFB2205678.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JQH22_001295"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JQH22_001295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFB2205679.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JQH22_001296"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JQH22_001296"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFB2205680.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JQH22_001297"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JQH22_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFB2205681.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="JQH22_001298"
     CDS             complement(5179..5421)
                     /locus_tag="JQH22_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFB2205682.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JQH22_001299"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JQH22_001299"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFB2205683.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JQH22_001300"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JQH22_001300"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFB2205684.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="JQH22_001301"
     CDS             complement(8338..9312)
                     /locus_tag="JQH22_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFB2205685.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="JQH22_001302"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="JQH22_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFB2205686.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="JQH22_001303"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="JQH22_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFB2205687.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="JQH22_001304"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="JQH22_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFB2205688.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="JQH22_001305"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="JQH22_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFB2205689.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="JQH22_001306"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="JQH22_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFB2205690.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="JQH22_001307"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="JQH22_001307"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFB2205691.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="JQH22_001308"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="JQH22_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFB2205692.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="JQH22_001309"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="JQH22_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFB2205693.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JQH22_001310"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JQH22_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFB2205694.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="JQH22_001311"
     CDS             23179..23382
                     /locus_tag="JQH22_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HFB2205695.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="JQH22_001312"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="JQH22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFB2205696.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="JQH22_001313"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="JQH22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFB2205697.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="JQH22_001314"
     CDS             26387..26659
                     /locus_tag="JQH22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFB2205698.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JQH22_001315"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JQH22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFB2205699.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JQH22_001316"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JQH22_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFB2205700.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JQH22_001317"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JQH22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFB2205701.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JQH22_001318"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JQH22_001318"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFB2205702.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JQH22_001319"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JQH22_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="JQH22_001320"
     CDS             complement(31906..32304)
                     /locus_tag="JQH22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HFB2205703.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="JQH22_001321"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="JQH22_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFB2205704.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="JQH22_001322"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="JQH22_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFB2205705.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="JQH22_001323"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="JQH22_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFB2205706.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="JQH22_001324"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="JQH22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HFB2205707.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="JQH22_001325"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="JQH22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFB2205708.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQH22_001326"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQH22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFB2205709.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9416 c         9897 g         9201 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccataccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgtttgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg tctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 aggcaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgattcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 taggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acagcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttccccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgt tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DADLDT020000028        36833 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000028 DADLDT020000000
VERSION     DADLDT020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.028"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..247
                     /locus_tag="JQH22_001327"
     CDS             <1..247
                     /locus_tag="JQH22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFB2205710.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JQH22_001328"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JQH22_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFB2205711.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JQH22_001329"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JQH22_001329"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFB2205712.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JQH22_001330"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JQH22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFB2205713.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JQH22_001331"
     CDS             complement(3258..3542)
                     /locus_tag="JQH22_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205714.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JQH22_001332"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JQH22_001332"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFB2205715.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JQH22_001333"
     CDS             complement(4443..5651)
                     /locus_tag="JQH22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2205716.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="JQH22_001334"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="JQH22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFB2205717.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JQH22_001335"
     CDS             complement(6446..8131)
                     /locus_tag="JQH22_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFB2205718.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLDFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JQH22_001336"
     CDS             8401..8778
                     /locus_tag="JQH22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFB2205719.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JQH22_001337"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JQH22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFB2205720.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JQH22_001338"
     CDS             9225..9512
                     /locus_tag="JQH22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFB2205721.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JQH22_001339"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JQH22_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JQH22_001340"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JQH22_001340"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFB2205722.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JQH22_001341"
     CDS             11269..11745
                     /locus_tag="JQH22_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFB2205723.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JQH22_001342"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JQH22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFB2205724.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="JQH22_001343"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="JQH22_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFB2205725.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JQH22_001344"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JQH22_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFB2205726.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JQH22_001345"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JQH22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFB2205727.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JQH22_001346"
     CDS             16302..16790
                     /locus_tag="JQH22_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFB2205728.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JQH22_001347"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JQH22_001347"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFB2205729.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JQH22_001348"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JQH22_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HFB2205730.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JQH22_001349"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JQH22_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFB2205731.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JQH22_001350"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JQH22_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFB2205732.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JQH22_001351"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JQH22_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2205733.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JQH22_001352"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JQH22_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFB2205734.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JQH22_001353"
     CDS             complement(22242..22757)
                     /locus_tag="JQH22_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFB2205735.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="JQH22_001354"
     CDS             23406..25175
                     /locus_tag="JQH22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFB2205736.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="JQH22_001355"
     CDS             25386..25709
                     /locus_tag="JQH22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFB2205737.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="JQH22_001356"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="JQH22_001356"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFB2205738.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="JQH22_001357"
     CDS             complement(26533..27546)
                     /locus_tag="JQH22_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFB2205739.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="JQH22_001358"
     CDS             complement(27645..29075)
                     /locus_tag="JQH22_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFB2205740.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JQH22_001359"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JQH22_001359"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFB2205741.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JQH22_001360"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JQH22_001360"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFB2205742.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JQH22_001361"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JQH22_001361"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFB2205743.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JQH22_001362"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JQH22_001362"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFB2205744.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JQH22_001363"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JQH22_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFB2205745.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JQH22_001364"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JQH22_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFB2205746.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9214 a         9156 c         9440 g         9023 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagtca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggataccta gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata actcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaa
//
LOCUS       DADLDT020000029        34944 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000029 DADLDT020000000
VERSION     DADLDT020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34944
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.029"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..2261
                     /locus_tag="JQH22_001365"
     CDS             <1..2261
                     /locus_tag="JQH22_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFB2205747.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3346
                     /locus_tag="JQH22_001366"
     CDS             2258..3346
                     /locus_tag="JQH22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HFB2205748.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="JQH22_001367"
     CDS             complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="JQH22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFB2205749.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="JQH22_001368"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="JQH22_001368"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFB2205750.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /gene="uxaA"
                     /locus_tag="JQH22_001369"
     CDS             6643..8130
                     /gene="uxaA"
                     /locus_tag="JQH22_001369"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFB2205751.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="JQH22_001370"
     CDS             8213..8764
                     /locus_tag="JQH22_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFB2205752.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="JQH22_001371"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="JQH22_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFB2205753.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="JQH22_001372"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="JQH22_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFB2205754.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12843)
                     /locus_tag="JQH22_001373"
     CDS             complement(11845..12843)
                     /locus_tag="JQH22_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFB2205755.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12922..13614)
                     /locus_tag="JQH22_001374"
     CDS             complement(12922..13614)
                     /locus_tag="JQH22_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFB2205756.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="JQH22_001375"
     CDS             complement(13687..14190)
                     /locus_tag="JQH22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFB2205757.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="JQH22_001376"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="JQH22_001376"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFB2205758.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /gene="higB"
                     /locus_tag="JQH22_001377"
     CDS             15696..16010
                     /gene="higB"
                     /locus_tag="JQH22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFB2205759.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="JQH22_001378"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="JQH22_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFB2205760.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="JQH22_001379"
     CDS             complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="JQH22_001379"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFB2205761.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21071)
                     /gene="ygjK"
                     /locus_tag="JQH22_001380"
                     /pseudo
     CDS             complement(18712..21071)
                     /gene="ygjK"
                     /locus_tag="JQH22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(21088..21507)
                     /locus_tag="JQH22_001381"
     CDS             complement(21088..21507)
                     /locus_tag="JQH22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205762.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21485..21838)
                     /locus_tag="JQH22_001382"
     CDS             complement(21485..21838)
                     /locus_tag="JQH22_001382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205763.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21839..22159)
                     /locus_tag="JQH22_001383"
     CDS             complement(21839..22159)
                     /locus_tag="JQH22_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205764.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22293..23726)
                     /locus_tag="JQH22_001384"
     CDS             complement(22293..23726)
                     /locus_tag="JQH22_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2205765.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23789..24238)
                     /locus_tag="JQH22_001385"
     CDS             complement(23789..24238)
                     /locus_tag="JQH22_001385"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFB2205766.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24235..27312)
                     /gene="ebgA"
                     /locus_tag="JQH22_001386"
     CDS             complement(24235..27312)
                     /gene="ebgA"
                     /locus_tag="JQH22_001386"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFB2205767.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNVRPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27494..28477)
                     /gene="ebgR"
                     /locus_tag="JQH22_001387"
     CDS             complement(27494..28477)
                     /gene="ebgR"
                     /locus_tag="JQH22_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFB2205768.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28695..29026
                     /locus_tag="JQH22_001388"
                     /pseudo
     CDS             28695..29026
                     /locus_tag="JQH22_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29068..30447)
                     /gene="ygjG"
                     /locus_tag="JQH22_001389"
     CDS             complement(29068..30447)
                     /gene="ygjG"
                     /locus_tag="JQH22_001389"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFB2205769.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30865..32385
                     /gene="aer"
                     /locus_tag="JQH22_001390"
                     /pseudo
     CDS             30865..32385
                     /gene="aer"
                     /locus_tag="JQH22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32539..33162)
                     /gene="nfeR"
                     /locus_tag="JQH22_001391"
                     /gene_synonym="yqjI"
     CDS             complement(32539..33162)
                     /gene="nfeR"
                     /locus_tag="JQH22_001391"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFB2205770.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33439..34184
                     /gene="nfeF"
                     /locus_tag="JQH22_001392"
                     /pseudo
     CDS             33439..34184
                     /gene="nfeF"
                     /locus_tag="JQH22_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34238..34313)
                     /locus_tag="JQH22_001393"
     tRNA            complement(34238..34313)
                     /locus_tag="JQH22_001393"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34278..34280),aa:Ile,seq:cat)
     gene            34438..34944
                     /gene="mug"
                     /locus_tag="JQH22_001394"
                     /pseudo
     CDS             34438..34944
                     /gene="mug"
                     /locus_tag="JQH22_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8539 a         8974 c         9177 g         8254 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcatgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacg gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccgtctt gccagacagt caggcggtcg aaattgctgg ccataaagtt gatgtattct
    20821 tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tcgggccgtc tggcaacaga
    20881 tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg
    20941 taatccttca tgtactgcgg cgcaccagta cggttaatca cgtttttata attgtcggcg
    21001 ttagcggcat gagcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt
    21061 ttgattttca tgagattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg
    21121 gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa
    21181 acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc
    21241 agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccttttaa
    21301 cgcttcatac atggcgctca ccatcatccc gctttcactt cccggtacgt ccagcccgtc
    21361 ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggggtaaaca ggcccgtgag
    21421 atagagttgt cctgtgccgt cggtaatttc cagaccattc atccaggtga tgccttcggc
    21481 aaggtcatac tggatgtaac cgttcatcca ttgccggata ggtgtatttg tcgtcgtagc
    21541 gatcgtactt gccaaaatgt agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg
    21601 cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgcg gtcataatgt
    21661 cgcgggtatt gggaatgacg tagcccaaat cgggtgtgaa gtcgccccac cattgcaggt
    21721 catccagcga ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat
    21781 aaccgccata gaaacggtta atcccacctt cgaaaccacc ccagctatgg tcaggcacct
    21841 aggcattacc gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc
    21901 aggagccgta cttcatctcc aggccacctt caatataggt gccgtcgctg tatttatgtt
    21961 tattctcacc ctctaaatcc atatatccac caagccctaa ttcgccgtaa aaacgaaatg
    22021 aaggagaaga tgtcgaggta attgcgggtg tttttgtgtc gtcatgttct gccggacgtg
    22081 cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat gctctgcatg
    22141 gtgcagtgat taatttcatt gtgttaatcc atgagctaaa gaaaaagtac agccttttat
    22201 cgttgtaatt ataaaaatat aaaggcagaa actattttcc ctctcctcat ccaggaagag
    22261 ggaaaatgcc tgctcatttt ttattagtct gcttaatcaa cattgctggc tggcgtcgct
    22321 tcaatatggc gctcttcagc cgttaatccc tttatatatt tgctgtattt ccaccacgca
    22381 aagccgagga agataacaat accgccgacg ttataaaaaa tgatggtcag aatattcgcg
    22441 ccagtcggga atgtcgaagc gacaaaccct acggcaaata tcgcaatcag cattgaaaca
    22501 acgataatac cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg atctaatttg
    22561 gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg gaagcatgga cgctgcggcg
    22621 gtcatattaa taatggtatt catcagatcc tgcacggtat tggaaccgag catcgggata
    22681 atcatcagcg ggatgacgat cagaaactgg atccatgctg cgcgcgccgg aacgccgttt
    22741 tcgttaagtt cgacggtttt cttaccaaag atgccttccg ggatttcgga gaagaaaatt
    22801 ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca ttgcggtaaa ggacaccaga
    22861 ccgacaaagc gattcatcaa cgcttccggc agaccaaaat acgccgccat gccgtggaat
    22921 acctgcaccg atccgccggt aaatttcaac tctttgctgc tgacgaagac gttaatcagc
    22981 accgaggaga cggaatacag tacgccgata aaaatcccgg cgaggatgat cactttaacg
    23041 aacgatttcg aaccgccttt gacgtcgtta acgtacaccg cgacggactc cgcgccacct
    23101 gcggccataa agatccaggt ggtaacgccg aggaacgccc agttgaagtt cgggatcatc
    23161 gcgtcaacgg tgatcggatc ggcaggctgt acgccgccaa ccagtgccgt acctgccagt
    23221 aaaatgtagg agagcgttaa cagcagcatc agcgttgaag tgacggaggt aattggcccc
    23281 agcattttcg ccccgttggt ggaaacccag gtggagaagg cgaacagcac catactgata
    23341 acggtggtgg caaccggcgt cataatatat tcgtagccga ggaaggcata cgaagcataa
    23401 gcaataacgc gcggcaacag cgaggtgaaa aagaacagat ttacgaacca gtaagtgtag
    23461 gcagtaataa atgcccaacg tccgcccagc gaacttttta cccacgcgta gacaccggct
    23521 tctgagtttt tatttaacga aacaaattct gcgatgatca gacagaaggg aataaaataa
    23581 aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc caagctcaat attattattg
    23641 ataacgttgt taaagctgta aacggcggca aaagtcagcg agagcaagcc gaatttgccg
    23701 attgtattac gtttggtatc agacataaca catctccgaa aatgtgtagg gtagcggtgg
    23761 cggcaaactc cttttgccgc cgtagttgtt atttgttatg gaaataacca tcttcgatgg
    23821 tgactttgag aaccactttt ttgaccgcgt tattgcagat aaaccgatac gcttcatgga
    23881 tatcgcaaat aacgatttgc ccttcgtgga cctctacggt ttctccgcag ccttttaaat
    23941 attcacggtc agtttcatca cgataatatt ccactacctg taatgtctct ttcggcgcat
    24001 attcaatttt ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg
    24061 taaatagcgc atcggtcgcg gagtcatttt ccacacggta ggtcaatgaa tcaccgatgg
    24121 agtgggcgac gccaggctga atgttgtcga tattttcaat cgcttcaacg cagcgttgcc
    24181 acttcttgcc agaggcgtaa atctggcgga actgttctaa gttatcgatg atcctcattg
    24241 cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag
    24301 gctttgcgcg gtagcttctc cgccagaaac cggcagcaac gtaaagccgt agctgaagtc
    24361 acggaaccag acgcgccagg agtccagcac ctcgctgccc caggagttgg agccgaggcc
    24421 aagtagctgg tggtcgaggt tcagggtgat gtcatcactg cgctgcagct cgttacagtg
    24481 ctgggcagcg tggatgtttt cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg
    24541 cggaaccacc agcagaccgt tgccgtggcg gttagtcagt gccgtccagc ggacatgctg
    24601 acggttgccg ttgttctgcg ggaagggata gttctcgaac atggcatcga cggtgctgcg
    24661 ccagatatcg atgatgttag cctgctggct gtcggcgtag ttttcgcccg gtccacgacc
    24721 gtaatacgcc acctgatcgt attcgccgtt aatccccatg gtgaaaccga tgcacggaat
    24781 gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc
    24841 agcgatgcgc cagatgtagg tacagcgcat cccgaagtca aacaccggcg gtgcaataac
    24901 tgtgcggctg atgatcaaca cttcaccatc gctctgttct acggcaaagt cgcgcagatg
    24961 ttcctgcatg atctgcaaat gattcggctg ccacaaccct tcgtactcct gcttgtggtt
    25021 gtcgatcatc ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt
    25081 tacctggcag gatgtcggtt tgccactcat ttttgagaag gtgatcgcga agttgtagcc
    25141 gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc ggacgcacat tatttggtgc
    25201 gaaaggcact ggctgcgcgg tgttttcctt cagcgggaac tgataagagg cgatagaatg
    25261 cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc
    25321 gcgggcgtcc agctgcggca gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc
    25381 gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag
    25441 ggtgtagtca tcaagcgtgg taaaccacag tttattttcg actttcaact cgccgcgagt
    25501 cagcgcgtgg atttttaccg gcgcgataac ctgtttgtac tctttcaggc ccgatcccgg
    25561 cgtctgatcg gaatagatca aaccatcaag acagaagtta tagttgttgg gatagtcgcc
    25621 gccgaattta taccagacat tgccgtggtc gtcctgtgcc tggatcccgt ggtcgcacca
    25681 ctcccagaca taatgaccct gaatgcaatc gtgcttatag aagacgttct ggtactccgt
    25741 cagcccgccc ggtccgttcc ccatcgcatg agcatattca cagatgatgc gcggcttcgg
    25801 atgcgggtat tcaccaaact cattcatcag cggcacgcgg gtgtacatgg tggaaataat
    25861 atcgaccact tcagcatcgc gatcttcttc gtaatgaacc agtcgcgtgt catccagcgc
    25921 tttcgccgca tggtacatcg cgcggatgtt acagccatag ccggattcat tgcccagcga
    25981 ccagatgatg atcgacggat ggtttttctg tgcgtggata tggcgaacaa tgcgctcgac
    26041 gtagactttt tcccactgcg gatcgtcggt aatacggctg atatcgccga cattagcaaa
    26101 gccgtgcgat tcgacgtcgg tttccgccat cacaaacagg ccgtagatat cacacagttc
    26161 gtaaaaacgc ggatcgttcg ggtagtgagc ggtacgcacg gagttgatat tgtgctgctt
    26221 catcaactgg agatctttct cgacgcgatc cattccaacg gcgcggcctt tgcgatgatc
    26281 gttgtcgtga cggttgacgc cgtgcagcat cacataacgg ttattgatcc agaacagacc
    26341 gtcgcgcact ttgatatcac ggaagccaac gcgttgtggc accacttcca gaacgttgcc
    26401 gttggcgtct ttcagcgtca tgaccagatg gtaaagataa ggggattctg ctgaccattg
    26461 ctgcggctgt tcgacagtaa aagcaaagct ggcgctggtc agtttttcaa ttgccaaatg
    26521 atcaatggcg ctgctgtgca ccacgcgttc gccatcaaac agggtatatt ccagcgtcgt
    26581 gacgacaggg gaggcggcga gattttccag caccacttcg caggaaagcg tggcatcgca
    26641 ataggcttcg tcaaagtcgg tacgcacagt gaaatcgtta atatgcgtta ggtgttttcc
    26701 gaccagataa acatcgcgga agatccccgc cgaccaccac atatcctggt cttccacgta
    26761 ggtagagtcc gcccactgca tcacgcgcac acacaacagg ttgtcgccgg ttttaaccat
    26821 cgcgctgatg tcaaactctg cggtcaggcg actgcccttg ctgaaaccca catactgacc
    26881 gttaacgtag acttcaaaat aggtttcgac gccgtcaaat ttaatcagcg tctgtttacc
    26941 ctgccagccg tcgctgaggg tgaaaatacg ttgataggca ccggttgggt tatcgctggg
    27001 gacaaacggc acatcgatgg ggaacggaaa accttcgtcg gtatattgca gtttgccgtg
    27061 accttccatt tgccacatgg cggggacggt aatatgcccc cagtcagcca ttaactcaga
    27121 ggtgaaggct tctggtactt gcagcggatg gtcaaaaaag tggaaattcc actgactgct
    27181 taagggcaga aacaggctgc tggtttcgcg ggcaaaggta cgcgcttgcg caacagaatc
    27241 atatgaaaaa aagtacgcac gcggcgcaag tcggttttcg tgggtgagct gaatgttttc
    27301 ccagcgattc atagggcctc ccggtagaga gaaatcggaa ccattcatgg tgatggtagt
    27361 ttgtgtaatt gctgggggtt agtaaaattt ttactaaaaa gcagcaggta aatgcattta
    27421 ctttaactgg atcacgaaaa taaacggcag cactgtccgg caaaaatgat tgccggacag
    27481 tgtgtaaggg gatttaacgg gtcgtgccgc gcagttttaa tttgctggga acgaagacta
    27541 gcagcggcag cgcgcgacca tcgcgggctt tttcatacac caggttaacg ccctgacttc
    27601 ccatcatctc ggaatggatg cgcacggtgg agagcggcgg aaaggtaaat cgcgcggtgg
    27661 ggatatcgtt aacgctgata agcgaaatat cttgtgggat gttcaggcca cgttcatgaa
    27721 ttgcccgcag tacgccgata gcaatggaat cggaagcaac aaacagtgcc ttcggatagt
    27781 cttcccgcgc cagcatttgt tttgccagtt cataacccga cgaactggaa aaaccgccgc
    27841 gccagatatc ctcttcgcgt accacttgtt tcagtcggcc atattccgca aaggcgacct
    27901 cacgaatatc cgccttgcca ggctcatctt caccgccaat aaaaccaata cgattaacgc
    27961 cctggttgat atagaagtcg atgatttctt tactgatgcg tgccagatcg atatccaccg
    28021 catcgtaacc gctgccgggt tcgtgaaagt cgataaaaca gatattgtcg gtcaacgcgc
    28081 tggcagcggc gcgcagggcg ggcgtgggtt tgccgacaat taaaataccg gtgacgtttt
    28141 taatgtctgg taatccgttg tgttcataac agttggtcag ttcgatcgcc agcttttcgc
    28201 actgagtttc aatgccgtgg cggatcgcca gatagtaagg atcgttgatc tccagctcct
    28261 gctggtagct gtagatagcc agaatatggt gttggttgac tgcgcctgtc tgtagtttac
    28321 gggcgctact ggtcttgtac tccagctttt cggcgatctc gagaatgcga tgtttcgtct
    28381 cttctttcac attcaatgtc ggatcgtcat ttaagaccct ggatactgtc gccagggata
    28441 cgccagcttc gattgcgatg tcttttaatg ttgccatgtt tacctttttt actttccctg
    28501 atgtgcgccc ccattgtaga ggaagtgctt atcgggtcac ctgttttcag taaataaagc
    28561 gcgtttcttt gacgctgtaa cggtaacaca gcggcttagg aatgtgatca cgcctgcatt
    28621 ctccattttt agcctacagg attgagaaaa acttttctat agtttagtaa aattttatcc
    28681 gaagggagga agcaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    28741 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    28801 ttgatgtggg gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga
    28861 ggagctgatg gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg
    28921 tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt
    28981 gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc
    29041 gacgtcgtat cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc
    29101 cagcgcctta cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg
    29161 cggttcaatg cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg
    29221 gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa tcgccatcaa
    29281 catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa
    29341 accgtccagc aacatatcgc ctttttgctc agcctgagcc ggtaagttct gctccagcaa
    29401 cacattgatg gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt
    29461 atgcaggaat gggttgtcga acagaactga aaacacctct tcagtggcga tggtcgcgcc
    29521 aatcggcatc acgccgccgc cgagggcttt tgccagacac agaatgtccg gctgaacgtt
    29581 ctcatgttcg caggcaaaca tcttgcccgt gcgccccatg cccgtttgta cttcatcgag
    29641 gatcatcagt gcgccgaact catcgcacag cttacgcacg gcggtgagat agcccggcgg
    29701 cggcagaatt acgccacctt cgccctgaat cggttcgagg atcaccgcag ccacatcatc
    29761 accggttttt ttgcactcat taagagccgt gcgcatggct tcgatattgc caaacggcac
    29821 atgacggaac cctggcagca gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc
    29881 cgacagcgcg ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa aagtaaattt
    29941 gccgcgcggc gactgataag ccttcgccag cttcagcgcc gcttcaacgg actcggtgcc
    30001 gctattacag aagaagctgt atttcagttt accgggcgtt agcgcagcaa gggttttcgc
    30061 cagcatagcc cgtaacggat cgagtagctc ctggctgtgc agtggttgtt tcgcaagttg
    30121 attctgtacg gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc
    30181 tcccaggcag tcgataaact cctgtccctg ggtgtcgaca agcgtattta aacctcccgc
    30241 ttgccactct acggctccgt aatccccgcc ggcggtaaca gatttgcgat actctaaaaa
    30301 ccccggattg acatgctctt tgaagtattc aatcacctct cggttaagtg ctttcatctc
    30361 ctcatgatcc agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc
    30421 cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata
    30481 ccgatttaag tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa
    30541 cacaaatgcg gttatcattt gtgcaatatt taatgctatc cgttaacatt tgttgttatt
    30601 ttgcacggtt tttgtcgggt atggatttga tgattgctgc gcaaatgcac gttttcaggg
    30661 caataattgc ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa
    30721 gtattttaaa tcaaagaatt agatttgtgc tttaatcgca aattgcgatc taaatcaaat
    30781 taatcggtta aagataaccg cagcggggcc gacataaact ctgacaagaa gttaacaacc
    30841 atataacctg cacaggacgc gaacatgtct tctcatccgt atgtcaccca gcaaaatacc
    30901 ccgctggcgg acgataccac tctgatgtcc actaccgatc tgcaaagcta tatcactcat
    30961 gctaatgaca cttttgtgca ggtgagcggc tttaccttgc aagagttaca agggcagccg
    31021 cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat gtggttcacc
    31081 ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat
    31141 tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg
    31201 tcgattcgta cccgggcgac ggatgaagag attgcggcgg tggagccgct gtacaaagcg
    31261 ttgaactccg gacgtaccag taagcgtatt cataaaggcc tggtggtgcg taaaggctgg
    31321 ctgggtaaac tgccttcatt accgcttcgc tggcggacgc gtggagtgat gaccctgatg
    31381 tttatcttgc tggcggccat gctttggttt gttgctgccc cggtggtgac gtatttcctc
    31441 tgtgtgttag tggtattgtt ggcaagcgct tgttttgaat ggcagattgt gcgcccgata
    31501 gaaaatgtcg cccgtcaggc actgaaggtg gcgaccggag agcgtaatag tgttgagcac
    31561 ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg tagggcagct tggcctgatg
    31621 tgccgttggc taattaacga tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag
    31681 acgctggcga aaggcaccga tgaactgaac gaacataccc agcagacagt tgataacgtt
    31741 cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg
    31801 gcgtcggctg ccgataaact ttctatcacc gccagtaatg cggcagtgca gggtggggag
    31861 gcgatgacca cggtgatcaa gacaatggac gatatcgccg acagtaccta gcgcattggc
    31921 accattactt cgctgattaa cgatattgcg tttcagacca atattctggc cctgaatgcg
    31981 gcggtggaag cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa
    32041 gtgcgtcatt tagccagccg cagcgctaat gctgccaacg atattcgcaa gctgattgat
    32101 gccagtgctg ataaggtgca atccggttcg cagcaggtac acgccgccgg acggacgatg
    32161 gaagatattg tggcacaggt gaaaaacgtc acccagttga tcgcccagat tagccattca
    32221 acgctggaac aggccgatgg gctttccagc ctgacccgtg cagtggatga gcttaacctg
    32281 atcacccaga aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa
    32341 caccgcgcca gccgactgga agacgcggtg acggtgctgc attaatcggt atgccggatc
    32401 cggcggttgg agcacaatgc ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg
    32461 tgtagcgggt gtaggccgga taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc
    32521 cactggcgga tgcggcgatt aatccagttg cgtaatatca aaagcggcgc ggtcgatcac
    32581 cgcaatgatc ttttttattt gtgcatcact gatatcgctc tggttgacgc gtaaatccag
    32641 caccgcttta aaattatcca gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc
    32701 aacgcaacgc gctttgatgc gttcttcaat catctccact tgttcgcggt tttcttccag
    32761 ccactgcgca ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct cttcgcggat
    32821 ggtaatcagc gactgctcct gcagaaaatc cagcgtcggg tagatgacgc ccgggcttgg
    32881 ggtgtaattc ccctgggtta gattctcaat cgctttaatc aattcgtaac cgtggctgtc
    32941 atcgcgcgag agaatatcca gaatcaccag acgcaattca ccgtgaccaa agaaacgttg
    33001 ccgacgaccg ccgccgcgac cgtggcgacc accgcaacat tgaccgtgtt catgctggtg
    33061 accgtgtccg cagtgctcgt gttctgactt ctcacctttg cagcagccct catgccgtgg
    33121 ctggccttca tgtttacaac acccttcgtg atgatggctc atatcagcct ccttttgctt
    33181 gattagatat atctaattta tatctgatgt gagcattact gcaagtacaa aaatgatttt
    33241 taaacgtgga agcacctcac atctaggccg gataagacgt tcacgccgca tccggcagca
    33301 ttgctatggc actgaatgcc ttcacatact tcagacatat cagttctaca aatcgcttgc
    33361 atttatcatg attaacaatc attatcattt gcgaatttta tttagatata tctgattacc
    33421 atcacgaagg cgataacaat gaataacacc ccccgctacc cgcagcgcgt tcgcaatgat
    33481 ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca tcagcgccgg ttttcagcgc
    33541 attgtccttg gcggcgaggc gttggacggt tttacatcgc gcggctttga cgatcacagc
    33601 aaactcttct ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagagc
    33661 atcgtctggc aggaaggacc acgcccaccg tcgcgtgact atacgccact gtatgacgaa
    33721 ctacgccatg aactggcgat cgatttcttt attcacgacg gtggggtcgc cagcggctgg
    33781 gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt
    33841 atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt
    33901 tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct
    33961 gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc
    34021 aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga
    34081 taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg
    34141 acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga
    34201 aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa
    34261 ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag
    34321 cggattataa agtaactccg tgtcgcaatc cagccattac cgcgcgcctg ctgtttttat
    34381 aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg agtaaatatg
    34441 gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt
    34501 tcatccgccg ggactggttt tccctttgct catccggcaa atcgcttctg gaaggtgata
    34561 tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat
    34621 tatcgttgtg gcatcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca
    34681 aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga ttatcagccg
    34741 caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcta gcgcggtgca
    34801 cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc
    34861 agcggtttaa gtcgcgtttc actggagaaa ctggttgaag cgtatcgcga gctggaccag
    34921 gcgctggtag tgcgtgggcg ataa
//
LOCUS       DADLDT020000030        34774 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000030 DADLDT020000000
VERSION     DADLDT020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34774
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.030"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(18..93)
                     /locus_tag="JQH22_001395"
     tRNA            complement(18..93)
                     /locus_tag="JQH22_001395"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JQH22_001396"
     tRNA            complement(152..228)
                     /locus_tag="JQH22_001396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JQH22_001397"
     CDS             complement(331..1716)
                     /locus_tag="JQH22_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2205771.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQH22_001398"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQH22_001398"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFB2205772.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQH22_001399"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQH22_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFB2205773.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQH22_001400"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQH22_001400"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFB2205774.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQH22_001401"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQH22_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFB2205775.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JQH22_001402"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JQH22_001402"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFB2205776.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JQH22_001403"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JQH22_001403"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFB2205777.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQH22_001404"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQH22_001404"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFB2205778.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQH22_001405"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQH22_001405"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFB2205779.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQH22_001406"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQH22_001406"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFB2205780.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQH22_001407"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQH22_001407"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HFB2205781.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQH22_001408"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQH22_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFB2205782.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQH22_001409"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQH22_001409"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFB2205783.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQH22_001410"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQH22_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFB2205784.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQH22_001411"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQH22_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFB2205785.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQH22_001412"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQH22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFB2205786.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JQH22_001413"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JQH22_001413"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFB2205787.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JQH22_001414"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JQH22_001414"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFB2205788.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQH22_001415"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQH22_001415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFB2205789.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="JQH22_001416"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="JQH22_001416"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFB2205790.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JQH22_001417"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JQH22_001417"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFB2205791.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="JQH22_001418"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="JQH22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFB2205792.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JQH22_001419"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JQH22_001419"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFB2205793.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JQH22_001420"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JQH22_001420"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFB2205794.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JQH22_001421"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JQH22_001421"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFB2205795.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JQH22_001422"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JQH22_001422"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFB2205796.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="JQH22_001423"
     CDS             complement(29521..31167)
                     /locus_tag="JQH22_001423"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFB2205797.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="JQH22_001424"
     CDS             complement(31170..31220)
                     /locus_tag="JQH22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205798.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="JQH22_001425"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="JQH22_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFB2205799.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33276
                     /locus_tag="JQH22_001426"
     CDS             31756..33276
                     /locus_tag="JQH22_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFB2205800.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="JQH22_001427"
     CDS             complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="JQH22_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFB2205801.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33758..34597
                     /gene="hdfR"
                     /locus_tag="JQH22_001428"
     CDS             33758..34597
                     /gene="hdfR"
                     /locus_tag="JQH22_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFB2205802.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34693..34768)
                     /locus_tag="JQH22_001429"
     tRNA            complement(34693..34768)
                     /locus_tag="JQH22_001429"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34733..34735),aa:Trp,seq:cca)
BASE COUNT         8477 a         9517 c         8781 g         7999 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccggtactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgttcgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 atattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg tacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtggaatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc gatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatta ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca accggctctc ggggcccttt
    32881 ctcgaccgct tcgatctctc actggagatc ccattaccac cccccggcat tttgagtaaa
    32941 acggtagtgc cgggagaaag cagcgccacc gttaaacaac gcgtaatggc cgccagagag
    33001 cgccaattta agcggcagaa taaactgaat gcctggctgg atagtccgga aatacgccaa
    33061 ttctgcaaac ttgagagcga agatgcgatg tggttggaag aaacgctgat ccatctgggg
    33121 ttatcgattc gtgcctggca gcggttactg aaagttgcac gaaccattgc tgatattgat
    33181 cagtctgaca ttatcacacg tcagcatttg caggaggcag ttagctatcg tgcgattgac
    33241 cgtttgctca tccatctgca aaaactactg acataaaaaa agggcatttc gcccttttta
    33301 ttaatcgtca gaatcggtgt agtcttcagc accttcaacc tgcggtttac cgccggaaag
    33361 ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac ttggaccaca cgcgttctgc
    33421 ttctgtcact ggctcacgtt cgccacggca tactgctacg aagagtttct cttcctcggt
    33481 aaccggctcg cgtttgccaa gatccaactc attgaaggca taaccatgac gctcaagcag
    33541 ttgtgcctct ttgatggtga aatcaccatg acgagagaat ccacgtggat aatgtttatt
    33601 gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt
    33661 gaccgagcta gttatggcgc ggagtattag ttacgcttga cagagtgtaa aacaaaacat
    33721 ttaaatcata acgacaaata attttgcgga gagcactgtg gatacggaat tgttaaaaac
    33781 tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac
    33841 ccagtcagca gtgagctttc gaatcagaca actggaaaat caactgggtg tgaacctttt
    33901 cacccgccac agaaacaata tccgtttaac cgctgccggt gaaaaactac tgccttatgc
    33961 agaaacgctc atgagcacct ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca
    34021 taacgagttt tctatcggtg ccagcgcctc gttgtgggaa tgtatgctta atcagtggtt
    34081 gggacgcttg tatcaaaatc aggatgccca tacaggctta cagttcgaag cgcgaattgc
    34141 ccaacggcag tctctggtaa aacagctgca tgaacgccag cttgatcttc ttattaccac
    34201 tgaagcgccc aaaatggacg aatttagtag tcagttgctg gggtatttca ctttagcgct
    34261 ttataccagt gccccttcta aactaaaggg agatcttaac tatctgcgac tggagtgggg
    34321 gccagatttt caacagcatg aggcaggttt gatcggtgct gacgaagttc ccattctgac
    34381 aaccagttct gctgaactgg cacagcaaca gattgcgatg cttaatggtt gcacctggct
    34441 acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc gttgtcgata gcacaacact
    34501 ttcacggccg ctttatgcca tatggctgca aaatagcgat aaaaatgcgt tgattcgcga
    34561 tcttttgaaa attaacgtgc tggatgaagt gtattaatct gaatggctgg caaggatgcc
    34621 ggtagaagga tttacttcgg agaggattat ttcagataaa aaaaatcctt agctttcgct
    34681 aaggatgatt tctggcaggg gcggagagac tcgaactccc aacacccggt tttggagacc
    34741 ggtgctctac caattgaact acgcccctaa ttag
//
LOCUS       DADLDT020000031        34660 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000031 DADLDT020000000
VERSION     DADLDT020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.031"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1128)
                     /locus_tag="JQH22_001430"
     CDS             complement(<1..1128)
                     /locus_tag="JQH22_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFB2205803.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVGNAANLLI"
     gene            complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="JQH22_001431"
     CDS             complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="JQH22_001431"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFB2205804.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="JQH22_001432"
     CDS             complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="JQH22_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFB2205805.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="JQH22_001433"
     CDS             complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="JQH22_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFB2205806.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="JQH22_001434"
     CDS             complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="JQH22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFB2205807.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="JQH22_001435"
     CDS             complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="JQH22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFB2205808.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3432..4409
                     /gene="pspF"
                     /locus_tag="JQH22_001436"
     CDS             3432..4409
                     /gene="pspF"
                     /locus_tag="JQH22_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFB2205809.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="JQH22_001437"
     CDS             complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="JQH22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFB2205810.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="JQH22_001438"
     CDS             complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="JQH22_001438"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFB2205811.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="JQH22_001439"
     CDS             complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="JQH22_001439"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFB2205812.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="JQH22_001440"
     CDS             complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="JQH22_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFB2205813.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="JQH22_001441"
     CDS             complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="JQH22_001441"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFB2205814.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10311..11729
                     /gene="puuA"
                     /locus_tag="JQH22_001442"
     CDS             10311..11729
                     /gene="puuA"
                     /locus_tag="JQH22_001442"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFB2205815.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11878..12042
                     /locus_tag="JQH22_001443"
     CDS             11878..12042
                     /locus_tag="JQH22_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205816.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12032..13417
                     /gene="puuP"
                     /locus_tag="JQH22_001444"
     CDS             12032..13417
                     /gene="puuP"
                     /locus_tag="JQH22_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFB2205817.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13551..13796
                     /locus_tag="JQH22_001445"
     CDS             13551..13796
                     /locus_tag="JQH22_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFB2205818.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14109..15752
                     /gene="sapA"
                     /locus_tag="JQH22_001446"
     CDS             14109..15752
                     /gene="sapA"
                     /locus_tag="JQH22_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HFB2205819.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15749..16714
                     /gene="sapB"
                     /locus_tag="JQH22_001447"
     CDS             15749..16714
                     /gene="sapB"
                     /locus_tag="JQH22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HFB2205820.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16701..17591
                     /gene="sapC"
                     /locus_tag="JQH22_001448"
     CDS             16701..17591
                     /gene="sapC"
                     /locus_tag="JQH22_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFB2205821.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17591..18583
                     /gene="sapD"
                     /locus_tag="JQH22_001449"
     CDS             17591..18583
                     /gene="sapD"
                     /locus_tag="JQH22_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFB2205822.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18585..19391
                     /gene="sapF"
                     /locus_tag="JQH22_001450"
     CDS             18585..19391
                     /gene="sapF"
                     /locus_tag="JQH22_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFB2205823.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19459..19812
                     /locus_tag="JQH22_001451"
     CDS             19459..19812
                     /locus_tag="JQH22_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFB2205824.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20180..20968
                     /gene="fabI"
                     /locus_tag="JQH22_001452"
     CDS             20180..20968
                     /gene="fabI"
                     /locus_tag="JQH22_001452"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFB2205825.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21112..22239
                     /locus_tag="JQH22_001453"
     CDS             21112..22239
                     /locus_tag="JQH22_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFB2205826.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22307..24241
                     /gene="rnb"
                     /locus_tag="JQH22_001454"
     CDS             22307..24241
                     /gene="rnb"
                     /locus_tag="JQH22_001454"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFB2205827.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24319..24421)
                     /locus_tag="JQH22_001455"
                     /pseudo
     CDS             complement(24319..24421)
                     /locus_tag="JQH22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24475..26449
                     /gene="pdeR"
                     /locus_tag="JQH22_001456"
                     /pseudo
     CDS             24475..26449
                     /gene="pdeR"
                     /locus_tag="JQH22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26597..26770
                     /locus_tag="JQH22_001457"
     CDS             26597..26770
                     /locus_tag="JQH22_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205828.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26860..27609
                     /locus_tag="JQH22_001458"
     CDS             26860..27609
                     /locus_tag="JQH22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HFB2205829.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27878..28096
                     /gene="osmB"
                     /locus_tag="JQH22_001459"
     CDS             27878..28096
                     /gene="osmB"
                     /locus_tag="JQH22_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFB2205830.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="JQH22_001460"
     CDS             complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="JQH22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFB2205831.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="JQH22_001461"
     CDS             complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="JQH22_001461"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFB2205832.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="JQH22_001462"
     CDS             complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="JQH22_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFB2205833.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="JQH22_001463"
     CDS             complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="JQH22_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFB2205834.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="JQH22_001464"
     CDS             complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="JQH22_001464"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFB2205835.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32045..32635
                     /gene="ribA"
                     /locus_tag="JQH22_001465"
     CDS             32045..32635
                     /gene="ribA"
                     /locus_tag="JQH22_001465"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFB2205836.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32699..>34660)
                     /gene="acnA"
                     /locus_tag="JQH22_001466"
     CDS             complement(32699..>34660)
                     /gene="acnA"
                     /locus_tag="JQH22_001466"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFB2205837.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEP
                     IGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8172 a         9039 c         8750 g         8699 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg
      781 aatatcctgc cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaatcc
      841 atcatcagat gaccatgggt aaaaaggtaa taaatgaaca tgtgaaaaaa tgctttgcag
      901 ccattgatgg tagaactgat taaatatggg tagtggtttt aaatcattac tgtgaaattg
      961 atcggcatag gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt
     1021 aattaatcgt ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa
     1081 tgttccaccg tagatttcgt ccatgtaatc cgtaattttc tgtttcatgt tgcctttccg
     1141 cggtagcggt cccatgaggc gagagactac tccgcagaaa ataaactgtc aattgagcac
     1201 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
     1261 acgcaaataa tctataggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg
     1321 ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac
     1381 cggcgttctc aacgtgggta tatcccatct cgctaaggag ctcttttgcc tgcccggact
     1441 ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa
     1501 tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat
     1561 actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca
     1621 ccagtgccag agcaagtaag ccttttttta aacatgtaag cgtccttcta tggatgaaaa
     1681 ttgccatcca ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat
     1741 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
     1801 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
     1861 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca agcttcacct
     1921 tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct
     1981 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
     2041 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
     2101 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
     2161 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac
     2221 cggtacatca aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg
     2281 aatacgccat aactttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
     2341 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
     2401 tcaaccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
     2461 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga
     2521 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga
     2581 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
     2641 gttcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
     2701 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
     2761 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
     2821 acgagtttgc cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
     2881 atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg
     2941 tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgctg
     3001 cacgtgccag atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
     3061 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcac
     3121 gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgca
     3181 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
     3241 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
     3301 cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat
     3361 ctgattgaag aatcaacagc aacatgccag gatgaattag cgaattacac taacaagtgg
     3421 cgaatttcat catggccgaa tacaaagata atttacttgg tgaggcgaac agctttctcg
     3481 aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg
     3541 aacgcggcac cggtaaagag ttgattgcca gccgcctgca ttatctctcc tcccgttggc
     3601 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
     3661 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg
     3721 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc
     3781 aggaaaaatt attgcgtgtg attgagtacg gtgaactgga gcgcgttggc ggtagccagc
     3841 cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
     3901 atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta gtgcaactgc
     3961 cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt gccattcaga
     4021 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
     4081 tgctgaatta tcgttggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
     4141 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
     4201 ggcgtccacc tgaagaagcc atcgccgttt cagaaaatac ctcgcttccg acgctgccgc
     4261 tggatttacg cgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
     4321 aggggaaata taaccagaag cgcgcggctg aattactggg gttaacttat catcagttcc
     4381 gcgcgttgtt gaaaaagcac cagatttagc gaagttgagc aatacaactt gccggatgcg
     4441 gcgtgaatgc tttattcggc atacataatc aatacgtcgt ttgcaggcct gataagcgca
     4501 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
     4561 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaagcga atcacgttgc
     4621 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
     4681 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg
     4741 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
     4801 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatat
     4861 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
     4921 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
     4981 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
     5041 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
     5101 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
     5161 cgcactcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
     5221 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg
     5281 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
     5341 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
     5401 caatcacgcc aggtcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
     5461 ccgcgccggt agtgaagaat gcggttttag cctgcccgct cactggggca agggtgttaa
     5521 ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa
     5581 actgttgcaa ttgctgctcc accgccgcga ccagatcagg gtggcgatgc ccggtattca
     5641 gcaccgcaat gcctgcggcg aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag
     5701 cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
     5761 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
     5821 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc gcccatcgcg
     5881 gtaaatggca cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
     5941 gcatcgaaac gctcggcgtc accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg
     6001 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcaaggcgg
     6061 ccgaactgcg gcatgtgcga caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt
     6121 ttcacgccct tcaactgagg gaaggttttt agtagtttcg gtaccacaag acgctcaacg
     6181 tcatccgggt tgcgtgcacc atacactaca cccccgccgt acagcaggcg gttgtcggcg
     6241 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
     6301 agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt gccgcacggc
     6361 atgctgcgtt tcgctagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
     6421 atcacatact tcgccgtcac ctgaccattg gcagtccgaa ccacggctgg cgtggtgtgc
     6481 tggatctgcg tcacggcgga aagctcatac acgcgcccgc cgttcaggcg gatggcatcg
     6541 gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc cagcagcgca
     6601 ccagtgtagc gatcgctggc gacctcgcgg cgaatagcgt tgacgtccag cagctccagc
     6661 tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc gagttgttta
     6721 tcattcatcg ccacaaacag accgccgggg cggtagtcgc agtcaatctg ataacgtttg
     6781 atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat acgggcggtg
     6841 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
     6901 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
     6961 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
     7021 caaacgtcgc aggtgattga ctcattcagt gtgtcgaatg gtgcatattt attcgcactg
     7081 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
     7141 tttcagttca gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
     7201 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
     7261 accggctttc aggcgtcggc tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc
     7321 cgcgccaagg ccgtactggc tgtcgttggc aagctgcagc gcctgatctt ctgatgtgaa
     7381 acgggtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
     7441 cacatccacg aagatggtcg ggccgatggc cgctgccagc ccggcgttgc gaccatccag
     7501 cagcagttgc cccttgcctt cgccttcacg aataaaggtg tgtaccgagt cagcgtgagc
     7561 gctgtcgatt aaggtgccca tggtggttgc gggatcaagt ggatggcccg gctgccagtt
     7621 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagtaacaa
     7681 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
     7741 tgccgctttt tgcagatccg gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc
     7801 ttccagccag acacgtttca tgttgctatc acccgcgtct ttcagcagtt gtttacccgt
     7861 acgcgtggag ccggtaaagg cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc
     7921 cgcttcatga ccaaaacccg tcaccacgtt caacacacca tccggcaagc ctgcttcttt
     7981 cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt ttagaatcac
     8041 gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcaaca gcgggaagtt
     8101 ccacggcacg atggcggcaa tcacgccgac cggttcacgc acgatcatcg ccagctcatg
     8161 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
     8221 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
     8281 gagagtttcc agcagtgcca gctcttcggc gtgggcttcc attaaatcgg cgagtttatt
     8341 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
     8401 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
     8461 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
     8521 ttcaccgtta ataaataagc ggttttcaat ggcgagactt aacgctttat cctgccagta
     8581 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
     8641 gtgaacgtca gggcgcagag atccccgggc tgcatgcccg tttatttctg gcgacattct
     8701 ttatggattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
     8761 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
     8821 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
     8881 atcgtcagaa caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt
     8941 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
     9001 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg
     9061 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
     9121 agtgacaaac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
     9181 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
     9241 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
     9301 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
     9361 ggtgctgcca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
     9421 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
     9481 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
     9541 ccccttgtcc atgtagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
     9601 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
     9661 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
     9721 aaccaccggt tgccaccacc agttcttgta aaccccggca gatggcgaaa atggggatgc
     9781 gcctttcgag tgcggcatta attatcgcca tgctcagaag atcacgcccg ggatcggcgt
     9841 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctactaggaa
     9901 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat
     9961 gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    10021 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    10081 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    10141 gctttaaatg acctttatgt tcaatatttt ttcaatctag cagtggattt tcatttttgc
    10201 aaactcaatt taacatttga caaacatttg gtttgcatac agattcgaat gatggtcatt
    10261 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    10321 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcaccttta
    10381 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    10441 ttactgattt gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagc
    10501 tcgagaaagg gtgttatttc ccggcctcgg tatttgcaat ggatattctg ggcaacgtag
    10561 tagaagaagc aggtctgggt caggaaatgg gcgagccgga tcgtacctgt gttcctgttc
    10621 tcggttcctt aactccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    10681 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    10741 ggcagcagct gcgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt
    10801 tactggatcg ccagcgcgac gctgaagggg atctgcaacc gccctgcgcg cctggcaccg
    10861 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg
    10921 tgctcaatga tattgatgaa ctggcgcagt tgcagctgat tccggcagat ggcgcggtcg
    10981 ctgaggcctc gccgggtcag tttgaaatca acctttacca tactgataac gtgctggaag
    11041 cctgcgatga tgcgctggca ctaaaacgcc tagtgcgtct gatggcagaa aagcataaga
    11101 tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcggg atgcatatcc
    11161 atatcagtat gcaaaataat cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt
    11221 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt
    11281 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc
    11341 aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggtgaccgtc
    11401 ataatcaccg cgtggaatat cacgtggcgg gtgccgatgc caacccatat ctggtgatgg
    11461 cagcgatttt tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag
    11521 tcgaaggcaa cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc
    11581 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    11641 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    11701 aaattgagtg gatgttgaaa aacgcgtaat tccccttgcg gggttgtttc atcggcctga
    11761 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    11821 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    11881 ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac
    11941 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    12001 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    12061 tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    12121 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    12181 tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    12241 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    12301 gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    12361 tctctttttg ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    12421 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    12481 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    12541 catggtggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    12601 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    12661 tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    12721 gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg
    12781 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    12841 taaagacccg gacgccgcac tgcctgaaat tgcgctgtac gtcggcggca agctctttca
    12901 gtcgattttc ctctgcacca cgtttgtgaa cacattagcg tctggcctgg cttcacatgc
    12961 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    13021 tggctatgtg cacccaaaat ggcggactcc ggcactcaac gtcattatgg tcgggattgt
    13081 cgcgctgtcg gcgctgttct ttgatttagt caccgcgaca gcactgatta acttcggtgc
    13141 tctggtggcg tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg
    13201 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    13261 gacggtgggt gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg
    13321 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgct
    13381 gtacgaaggt gacagaacgc cggtgagcga aacgtaatag ccggatgcgc ggcgcattat
    13441 cctgcctgag ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    13501 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    13561 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    13621 tcgcagaggc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    13681 tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta
    13741 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    13801 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    13861 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    13921 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    13981 agatggcgac gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    14041 caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    14101 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    14161 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    14221 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    14281 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    14341 gcctgatgcc tgaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    14401 tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa
    14461 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    14521 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    14581 aaagcgtccg caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt
    14641 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    14701 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt
    14761 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    14821 ggcgcggtaa accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac
    14881 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    14941 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    15001 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac
    15061 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    15121 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    15181 aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc
    15241 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    15301 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    15361 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    15421 cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc
    15481 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg
    15541 cgctctcctc gcagcagctg gcagcgcgta ttgaagccta tgacgaagcg cagagtattc
    15601 tggcgcagga attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    15661 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    15721 gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    15781 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcactcc
    15841 acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct
    15901 gatccactgg gattttggtg tttccagcat taacggtcaa ccaattgccg aacagttaaa
    15961 agagctcttc ccggcgacga tagaactgtg catcctcgcc ttcggttttg cgctgattgt
    16021 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat
    16081 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    16141 actgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    16201 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    16261 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    16321 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactattg aagtgtatga
    16381 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    16441 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    16501 catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    16561 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    16621 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    16681 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    16741 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    16801 acggctgcgc gggactggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca
    16861 tcgaccagca atttctcggt tatcaattat tgccgccatc atggtcgcgc tatggcgaag
    16921 tttctttctt cctggggact gacgacctcg gacgtgatgt tttaagccgt ttactgagcg
    16981 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc
    17041 tggtactggg caccttcgcc ggggcgacac acggcctccg ctcagcggtg cttaaccata
    17101 ttctggatac cttactggcg atcccttcgc tgctgttggc aattatcgtt gtagcgtttg
    17161 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg
    17221 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    17281 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg
    17341 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    17401 tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    17461 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    17521 tgattagcgt gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg
    17581 gggtggaata atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga
    17641 tgagtgggtt aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    17701 tcttgttggt gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa
    17761 taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg
    17821 tctctccgca cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga
    17881 accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    17941 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    18001 tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta
    18061 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    18121 gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat
    18181 ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca
    18241 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    18301 agtggaaacc gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc
    18361 gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac
    18421 tctacccggc gctatcccat tgctggaaca gttaccgatt ggttgtcgtc tggggccacg
    18481 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    18541 tctctatgcc tgtcatttcc cgctgaacat ggagaaaaag tgagatgatc gaaacgctgc
    18601 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggttggttt cgtcgtcaga
    18661 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    18721 ttggcgagaa tggttcgggt aaatccacac tggcaaaaat gctggcggga atgatagaac
    18781 ctaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat tattccttcc
    18841 gtagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    18901 gtatctcaca aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc
    18961 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    19021 actatccgca tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga
    19081 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcaatgc
    19141 gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt
    19201 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    19261 agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    19321 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta
    19381 aagatcgcta attttctgaa tcctccttcc agattattca cctcattctg acaagagatt
    19441 aaatcttgtc agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc
    19501 aatctcactt tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat
    19561 ttcaaatttc gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg
    19621 gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc
    19681 aggcgcacta gctggcttga gtcgcagggc tggaccgtgc tgcgtttctg gaataacgag
    19741 attgattgta atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca
    19801 ccctcaccct gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat
    19861 ggagacagtg ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag
    19921 ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc
    19981 cctcgcccct ctggggagag ggttagggta aggggaaaag cgccccccct gccgcagcct
    20041 gctccggtcg gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt
    20101 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
    20161 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
    20221 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
    20281 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
    20341 attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat
    20401 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
    20461 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa
    20521 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
    20581 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
    20641 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
    20701 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
    20761 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
    20821 cccgattcgc cgtacagtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc
    20881 cgatctctct gccggtatct ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc
    20941 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg
    21001 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
    21061 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
    21121 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat
    21181 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg
    21241 atccttgatg aagagacgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt
    21301 gcagtggagt tgttccctga ccagcttacc gtgacccgtt cacaaacctt caccgcttat
    21361 gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
    21421 tactattcgg cgcgacttac gccgctcccc ggacctgatt cctccaggga aagtaatcat
    21481 cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
    21541 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg tctgggactg tgtgatcatt
    21601 aaccaaatca ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat
    21661 ctcgggcatc cggtacgctg gttacccggg ctggcgatac aaaactacgc cgacgcgtca
    21721 ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa
    21781 gagcacacaa aaagttcaac tgcagaactt tgccaactgg ccgaaacaca ctctctccac
    21841 cctatttcac tttcccttct cgaaaagttg ttaaacagca cacgggtcaa tgcacagccg
    21901 gataatcagc ttgcggcgtt gttatgcgcg cgtataaatg gcagtcctgc ttgtttttcc
    21961 acctgtatgg attcatcaaa tgaatataaa aaaatcagca cccttctgcg caagggcgaa
    22021 aatgaaatta accgatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata
    22081 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
    22141 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
    22201 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
    22261 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
    22321 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
    22381 ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcacaaa aaagttattt
    22441 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
    22501 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg
    22561 tttcgtgggt aaagttcagg gcaaaaatga ccgtctgacc atcgttcctg atcatccact
    22621 tttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg
    22681 cgactgggcg gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
    22741 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
    22801 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
    22861 aggtctggtt cgtgaagatc tgacctcgct ggattttgtc accatcgaca gtgccagcac
    22921 tgaagatatg gatgacgccc ttttcgctaa ggcgctgccg gatgacaaac ttcagctgat
    22981 tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc
    23041 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
    23101 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
    23161 catgacgctc tccgctgatg gcaccactga agataatatc gaattctttg ccgccaccat
    23221 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga
    23281 ctggaagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
    23341 acgccgcggc gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
    23401 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
    23461 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg
    23521 cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga
    23581 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
    23641 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
    23701 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc tctgaacccg gtcctcactt
    23761 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
    23821 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga
    23881 tgaaatcact gtccaaatgg ccgaacgtcg ccgtctcaac cggatggcag aacgtgatgt
    23941 tggcgactgg ttatacgcac gcttcctgaa agataaagcc gggaccggca cccgtttcgc
    24001 ggcggaaatt gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc
    24061 tatcgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
    24121 ggaaaacggc actgtacaaa ttaaaggtga aacggtttac aaagtaactg acgttattga
    24181 cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
    24241 atctcctttc acggcccatt cctcatggat gggccgttta tttccccgct ctaccttcgt
    24301 tatatccagg cagatttttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
    24361 gattacaaat aaatatataa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
    24421 tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg catcatgaaa
    24481 accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg
    24541 ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga acctgatcgt
    24601 acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc
    24661 agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga
    24721 aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt
    24781 gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc
    24841 gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt atgtgaagat
    24901 tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact gtttatgagc
    24961 cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca
    25021 tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct gtttcgcaat
    25081 aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac
    25141 attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac cgacagtatc
    25201 accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca
    25261 gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac
    25321 gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc
    25381 tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca
    25441 tccaatacct cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc
    25501 ctcccctttc gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc
    25561 tctcccgaac atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac
    25621 acagcgaagg aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg
    25681 gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg
    25741 gttatcgaaa atcacctagc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    25801 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg
    25861 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg
    25921 gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga
    25981 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    26041 tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    26101 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
    26161 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    26221 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    26281 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    26341 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    26401 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    26461 tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt
    26521 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc
    26581 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    26641 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    26701 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga agacaccgtg
    26761 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    26821 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    26881 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    26941 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    27001 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    27061 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    27121 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    27181 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    27241 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    27301 ggccctttga cacgccagtg catccagcag gtacatttca gcaaagcatt tattggtatt
    27361 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgcac cgatgtggtc
    27421 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    27481 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    27541 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    27601 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    27661 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    27721 aattattatc agcgatataa caggaagtaa ttatcacctg cgtgatataa ccctgcgcgc
    27781 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    27841 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    27901 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    27961 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    28021 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    28081 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    28141 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    28201 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    28261 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    28321 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    28381 atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    28441 ctgaatacgc accgctccat cgcctttagg gcgtactggg gccgctttgg gctcatcaat
    28501 acgcccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    28561 tgtaaagagg cgttgatcgc tttcagtgtc tgcgctggat ctaccgattg cgttaccggg
    28621 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    28681 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gtgtaaccag tttgaactcc
    28741 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    28801 tcacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    28861 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    28921 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    28981 ccggaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    29041 gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    29101 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttacc gaccttcaga
    29161 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcaca attatgataa
    29221 tcaagggcaa caaccacagg agaattcgta acggcgcggg aagaagatga agcagttaac
    29281 gtcatgacca gaccttcttg atgatgggca ccgggagtgg cgcgaaacag gtaaacggcg
    29341 agcattctac ccgtgaaaat aaaaaattaa cagttgcgat ttttccccta gcaggctttt
    29401 tctgctccgt cgttgccgga gcacaatggg acatcaataa ctagtatgtt gtaactaaag
    29461 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat agttgaccag
    29521 gcacgacaag acggacaatg ccagtagagg gtgtatgcag taaaaccaca tttctggcag
    29581 ctataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac catcagactc
    29641 tctttggcgc gcccttcttc cgcttcattt aagtggtaat ccattagctt atggaataca
    29701 cgcatggtcg gatgacgctg aagctggcgt gtaatataga cctgtgcagc ctcactaccg
    29761 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    29821 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    29881 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac gcgttgcaaa
    29941 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    30001 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    30061 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    30121 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    30181 tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    30241 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    30301 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    30361 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    30421 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    30481 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    30541 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    30601 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    30661 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    30721 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    30781 ccacaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa
    30841 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    30901 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    30961 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    31021 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    31081 taaggcctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gtgcaatttc
    31141 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    31201 caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    31261 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga tagcaatggt
    31321 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    31381 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    31441 ttgtggaata tttttctctt cagccaactg ttcttttact agatttccgc gttctgctcg
    31501 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccaacc agataacaaa
    31561 aggtcgtggt tcctgtactt tgtctttgat ccaggattta acgccttgtc caacaaggat
    31621 tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    31681 gagaaaccag ccgaacaaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    31741 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    31801 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtcg
    31861 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaatgcc
    31921 agttccaaga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    31981 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    32041 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    32101 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    32161 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    32221 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    32281 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    32341 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    32401 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    32461 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    32521 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    32581 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    32641 ttgcatcgtg taattcattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    32701 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    32761 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    32821 taagcgtcac cggaaccgtc gcgccaggtt gtagattttg cagatcgcca atatcaatct
    32881 tctcttcccc ggttagccct aacgttttac gcgtcacgcc ttgcggaaat tccagcggca
    32941 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    33001 cacgaattcc aagcagacga ggtccttttg ccgcccagtc acggcttgag cctgatccat
    33061 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcagcat
    33121 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    33181 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    33241 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    33301 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    33361 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    33421 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    33481 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    33541 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    33601 gtgccgatgg ccagatatct ttcagataaa ccggctcgcc tttgcgatca tggccgatag
    33661 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggtg
    33721 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    33781 cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    33841 gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca
    33901 gttcgtcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg actttcgaac
    33961 ccggtgccaa cgacgctttg acccacggtt gccgcttgag gccaagagtt acggcttttt
    34021 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    34081 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    34141 cgactggcag gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    34201 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    34261 cttcaacgtc attcatatcc agttctaaca tactggtaaa aatcggttca tcgcccgggt
    34321 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    34381 gcccgcttaa acgcatgtaa tcgagggtta ctgcatcgat tgggaagaag ccacaggtgg
    34441 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt agtgaatcca
    34501 gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    34561 taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtaagtttaa
    34621 agcccactac gtccgggata agcatggaaa ccggctggcc
//
LOCUS       DADLDT020000032        34086 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000032 DADLDT020000000
VERSION     DADLDT020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34086
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.032"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..578
                     /locus_tag="JQH22_001467"
     CDS             <1..578
                     /locus_tag="JQH22_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2205838.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="JQH22_001468"
     CDS             complement(575..1711)
                     /locus_tag="JQH22_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2205839.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="JQH22_001469"
     CDS             complement(1717..2040)
                     /locus_tag="JQH22_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFB2205840.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="JQH22_001470"
     CDS             2223..2366
                     /locus_tag="JQH22_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205841.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="JQH22_001471"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="JQH22_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFB2205842.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="JQH22_001472"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="JQH22_001472"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFB2205843.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="JQH22_001473"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="JQH22_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFB2205844.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="JQH22_001474"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="JQH22_001474"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFB2205845.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="JQH22_001475"
     CDS             complement(8959..9567)
                     /locus_tag="JQH22_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFB2205846.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="JQH22_001476"
     CDS             complement(9638..10723)
                     /locus_tag="JQH22_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2205847.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="JQH22_001477"
     CDS             complement(10726..11088)
                     /locus_tag="JQH22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFB2205848.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="JQH22_001478"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="JQH22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFB2205849.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="JQH22_001479"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="JQH22_001479"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFB2205850.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="JQH22_001480"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="JQH22_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFB2205851.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="JQH22_001481"
     CDS             complement(15380..15589)
                     /locus_tag="JQH22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205852.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="JQH22_001482"
     CDS             15873..16235
                     /locus_tag="JQH22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFB2205853.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="JQH22_001483"
     CDS             16779..17462
                     /locus_tag="JQH22_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFB2205854.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="JQH22_001484"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="JQH22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="JQH22_001485"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="JQH22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFB2205855.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="JQH22_001486"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="JQH22_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFB2205856.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="JQH22_001487"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="JQH22_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFB2205857.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="JQH22_001488"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="JQH22_001488"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFB2205858.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="JQH22_001489"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="JQH22_001489"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFB2205859.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="JQH22_001490"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="JQH22_001490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFB2205860.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="JQH22_001491"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="JQH22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFB2205861.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="JQH22_001492"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="JQH22_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFB2205862.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="JQH22_001493"
     CDS             complement(29422..29853)
                     /locus_tag="JQH22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFB2205863.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="JQH22_001494"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="JQH22_001494"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFB2205864.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="JQH22_001495"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="JQH22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFB2205865.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="JQH22_001496"
     CDS             32939..33898
                     /locus_tag="JQH22_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFB2205866.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34086)
                     /locus_tag="JQH22_001497"
                     /pseudo
     CDS             complement(33885..34086)
                     /locus_tag="JQH22_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8469 a         8930 c         8691 g         7996 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata accatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcg
//
LOCUS       DADLDT020000033        34043 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000033 DADLDT020000000
VERSION     DADLDT020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34043
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.033"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..835)
                     /locus_tag="JQH22_001498"
     CDS             complement(<1..835)
                     /locus_tag="JQH22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFB2205867.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="JQH22_001499"
                     /pseudo
     CDS             927..1013
                     /locus_tag="JQH22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1418..1894)
                     /locus_tag="JQH22_001500"
     CDS             complement(1418..1894)
                     /locus_tag="JQH22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFB2205868.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="JQH22_001501"
     CDS             complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="JQH22_001501"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFB2205869.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3329..4369
                     /gene="bioB"
                     /locus_tag="JQH22_001502"
     CDS             3329..4369
                     /gene="bioB"
                     /locus_tag="JQH22_001502"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFB2205870.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4366..5520
                     /gene="bioF"
                     /locus_tag="JQH22_001503"
     CDS             4366..5520
                     /gene="bioF"
                     /locus_tag="JQH22_001503"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFB2205871.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5507..6262
                     /gene="bioC"
                     /locus_tag="JQH22_001504"
     CDS             5507..6262
                     /gene="bioC"
                     /locus_tag="JQH22_001504"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFB2205872.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6255..6932
                     /gene="bioD"
                     /locus_tag="JQH22_001505"
     CDS             6255..6932
                     /gene="bioD"
                     /locus_tag="JQH22_001505"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HFB2205873.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7511..9532
                     /gene="uvrB"
                     /locus_tag="JQH22_001506"
     CDS             7511..9532
                     /gene="uvrB"
                     /locus_tag="JQH22_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HFB2205874.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="JQH22_001507"
     CDS             complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="JQH22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFB2205875.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10897..11041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11029..12018
                     /gene="moaA"
                     /locus_tag="JQH22_001508"
     CDS             11029..12018
                     /gene="moaA"
                     /locus_tag="JQH22_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFB2205876.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12040..12552
                     /gene="moaB"
                     /locus_tag="JQH22_001509"
     CDS             12040..12552
                     /gene="moaB"
                     /locus_tag="JQH22_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFB2205877.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12555..13040
                     /gene="moaC"
                     /locus_tag="JQH22_001510"
     CDS             12555..13040
                     /gene="moaC"
                     /locus_tag="JQH22_001510"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFB2205878.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13033..13278
                     /gene="moaD"
                     /locus_tag="JQH22_001511"
     CDS             13033..13278
                     /gene="moaD"
                     /locus_tag="JQH22_001511"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFB2205879.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13280..13732
                     /gene="moaE"
                     /locus_tag="JQH22_001512"
     CDS             13280..13732
                     /gene="moaE"
                     /locus_tag="JQH22_001512"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFB2205880.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13869..14573
                     /locus_tag="JQH22_001513"
     CDS             13869..14573
                     /locus_tag="JQH22_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFB2205881.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14778..15491
                     /locus_tag="JQH22_001514"
     CDS             14778..15491
                     /locus_tag="JQH22_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFB2205882.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15527..16483)
                     /locus_tag="JQH22_001515"
     CDS             complement(15527..16483)
                     /locus_tag="JQH22_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HFB2205883.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="JQH22_001516"
     CDS             complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="JQH22_001516"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFB2205884.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17721..18482)
                     /locus_tag="JQH22_001517"
     CDS             complement(17721..18482)
                     /locus_tag="JQH22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFB2205885.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18615..19025
                     /locus_tag="JQH22_001518"
     CDS             18615..19025
                     /locus_tag="JQH22_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFB2205886.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18987..20093)
                     /locus_tag="JQH22_001519"
     CDS             complement(18987..20093)
                     /locus_tag="JQH22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2205887.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20104..21237)
                     /locus_tag="JQH22_001520"
     CDS             complement(20104..21237)
                     /locus_tag="JQH22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2205888.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21230..22966)
                     /locus_tag="JQH22_001521"
     CDS             complement(21230..22966)
                     /locus_tag="JQH22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2205889.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="JQH22_001522"
     CDS             complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="JQH22_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFB2205890.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="JQH22_001523"
     CDS             complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="JQH22_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFB2205891.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24857..26044
                     /gene="rhlE"
                     /locus_tag="JQH22_001524"
                     /pseudo
     CDS             24857..26044
                     /gene="rhlE"
                     /locus_tag="JQH22_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="JQH22_001525"
     CDS             complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="JQH22_001525"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFB2205892.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26853..29003
                     /gene="dinG"
                     /locus_tag="JQH22_001526"
     CDS             26853..29003
                     /gene="dinG"
                     /locus_tag="JQH22_001526"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFB2205893.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29031..29993
                     /gene="ybiB"
                     /locus_tag="JQH22_001527"
     CDS             29031..29993
                     /gene="ybiB"
                     /locus_tag="JQH22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFB2205894.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30134..31219
                     /gene="hcxB"
                     /locus_tag="JQH22_001528"
     CDS             30134..31219
                     /gene="hcxB"
                     /locus_tag="JQH22_001528"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFB2205895.1"
                     /translation="MKSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31448..31708)
                     /locus_tag="JQH22_001529"
     CDS             complement(31448..31708)
                     /locus_tag="JQH22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFB2205896.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="JQH22_001530"
     CDS             complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="JQH22_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFB2205897.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="JQH22_001531"
     CDS             complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="JQH22_001531"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFB2205898.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33032..>34043)
                     /locus_tag="JQH22_001532"
     CDS             complement(33032..>34043)
                     /locus_tag="JQH22_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFB2205899.1"
                     /translation="SRIHPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNGGIRL
                     DNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTEN
                     GNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLL
                     TAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIK
                     NGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGARYIGSIHKGSDGAVGTPAF
                     TEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANM
                     HF"
BASE COUNT         8080 a         8729 c         9193 g         8041 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt
     1321 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
     1381 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg
     1441 atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt
     1501 catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt
     1561 cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
     1621 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
     1681 cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
     1741 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
     1801 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
     1861 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     1921 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
     1981 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     2041 ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat
     2101 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     2161 acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc
     2221 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg
     2281 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg
     2341 gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc
     2401 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     2461 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     2521 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     2581 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     2641 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg
     2701 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     2761 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     2821 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     2881 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     2941 tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg
     3001 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     3061 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     3121 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     3181 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     3241 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac
     3301 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     3361 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     3421 tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg
     3481 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     3541 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc
     3601 aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc
     3661 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     3721 gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa
     3781 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca
     3841 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg
     3901 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     3961 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     4021 gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg
     4081 gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     4141 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     4201 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     4261 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc
     4321 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     4381 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg
     4441 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     4501 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg
     4561 gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg
     4621 catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt
     4681 atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt
     4741 attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg
     4801 cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc
     4861 tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg
     4921 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     4981 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     5041 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg
     5101 ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     5161 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt
     5221 gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga
     5281 gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc
     5341 ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc
     5401 acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc
     5461 gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa
     5521 taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga
     5581 tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca
     5641 cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc
     5701 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc
     5761 cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga
     5821 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     5881 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     5941 gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt
     6001 tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca
     6061 gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg
     6121 tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     6181 attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt
     6241 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     6301 aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag
     6361 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     6421 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     6481 ccttcgcaga acccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat
     6541 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     6601 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     6661 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     6721 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     6781 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     6841 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     6901 ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc
     6961 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     7021 aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg
     7081 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     7141 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     7201 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     7261 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     7321 taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     7381 cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat
     7441 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     7501 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     7561 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctagcgc accagacgtt
     7621 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     7681 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     7741 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     7801 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     7861 acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat
     7921 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     7981 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     8041 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     8101 cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     8161 tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac
     8221 tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     8281 acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa
     8341 taacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     8401 ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     8461 accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt
     8521 cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg
     8581 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     8641 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     8701 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     8761 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     8821 ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg
     8881 aatggcggaa gatcttgccg aatatctcga agaacacggt gagcgcgtgc gttatcttca
     8941 ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt
     9001 cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct
     9061 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     9121 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag
     9181 cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     9241 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     9301 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     9361 tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca
     9421 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     9481 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     9541 cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat
     9601 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     9661 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     9721 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     9781 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     9841 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     9901 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     9961 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
    10021 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
    10081 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
    10141 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
    10201 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
    10261 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
    10321 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa
    10381 acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt
    10441 gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
    10501 attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
    10561 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
    10621 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
    10681 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa
    10741 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct
    10801 tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt
    10861 ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc
    10921 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
    10981 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
    11041 ctgactgatg catttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
    11101 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    11161 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    11221 gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc
    11281 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    11341 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    11401 gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    11461 atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    11521 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    11581 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    11641 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    11701 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    11761 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    11821 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    11881 gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    11941 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    12001 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    12061 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    12121 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa
    12181 gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    12241 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    12301 cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt
    12361 atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    12421 gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    12481 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    12541 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    12601 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    12661 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    12721 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt
    12781 gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    12841 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt
    12901 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    12961 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    13021 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    13081 gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct
    13141 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac
    13201 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    13261 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag
    13321 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    13381 caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    13441 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    13501 gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    13561 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    13621 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    13681 ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt
    13741 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct
    13801 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    13861 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccccgg gctggcttgc
    13921 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    13981 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    14041 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    14101 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    14161 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    14221 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    14281 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    14341 acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    14401 ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata
    14461 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg
    14521 acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt
    14581 tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac
    14641 aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat
    14701 acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc
    14761 taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc
    14821 acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg
    14881 caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac
    14941 caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca
    15001 acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga
    15061 ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg
    15121 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
    15181 gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat
    15241 taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca
    15301 ttatctcgca tggacgaaat aagctccttg aacaggcagg aaaatgccat agtgaagagt
    15361 tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg
    15421 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
    15481 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
    15541 catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca
    15601 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
    15661 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
    15721 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
    15781 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
    15841 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
    15901 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
    15961 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat
    16021 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
    16081 agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc
    16141 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
    16201 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
    16261 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
    16321 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
    16381 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
    16441 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt
    16501 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
    16561 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
    16621 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
    16681 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
    16741 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
    16801 gagattactg gaccctactg tcgcccagtg atcgaccatc aatgccactt tgccgtggag
    16861 cgggcggcgg cggtactcaa aaacctgaat gccgccttta accagatagt tatacagcaa
    16921 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
    16981 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
    17041 ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc
    17101 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
    17161 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
    17221 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
    17281 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
    17341 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
    17401 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
    17461 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
    17521 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
    17581 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
    17641 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
    17701 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
    17761 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
    17821 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
    17881 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
    17941 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
    18001 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
    18061 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
    18121 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
    18181 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
    18241 aaatcgctcc acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
    18301 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
    18361 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
    18421 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
    18481 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
    18541 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
    18601 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
    18661 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
    18721 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
    18781 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
    18841 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac
    18901 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
    18961 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
    19021 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
    19081 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
    19141 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
    19201 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
    19261 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
    19321 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
    19381 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
    19441 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
    19501 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
    19561 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
    19621 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
    19681 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
    19741 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
    19801 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
    19861 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
    19921 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
    19981 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
    20041 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
    20101 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
    20161 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
    20221 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
    20281 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
    20341 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
    20401 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
    20461 accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
    20521 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
    20581 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
    20641 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
    20701 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
    20761 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
    20821 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
    20881 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
    20941 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
    21001 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
    21061 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
    21121 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
    21181 cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc
    21241 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
    21301 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
    21361 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
    21421 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
    21481 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
    21541 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
    21601 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
    21661 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
    21721 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
    21781 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
    21841 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
    21901 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
    21961 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
    22021 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
    22081 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
    22141 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc
    22201 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
    22261 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
    22321 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
    22381 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
    22441 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
    22501 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
    22561 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
    22621 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
    22681 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
    22741 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
    22801 atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg
    22861 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
    22921 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
    22981 ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
    23041 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
    23101 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
    23161 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
    23221 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
    23281 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
    23341 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
    23401 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
    23461 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
    23521 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
    23581 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
    23641 cgatacccgg caagcatcag attatactgc gcctgtgcca ccgagacgcc cgctttcgcc
    23701 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
    23761 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
    23821 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
    23881 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
    23941 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
    24001 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
    24061 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
    24121 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
    24181 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
    24241 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
    24301 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
    24361 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
    24421 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
    24481 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
    24541 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
    24601 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
    24661 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
    24721 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
    24781 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
    24841 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    24901 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgcggt
    24961 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
    25021 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    25081 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
    25141 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    25201 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
    25261 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    25321 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    25381 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    25441 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    25501 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    25561 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    25621 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat
    25681 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    25741 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    25801 gcacgtcgtc aaccatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    25861 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    25921 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    25981 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    26041 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    26101 gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc
    26161 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    26221 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    26281 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    26341 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg
    26401 cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac
    26461 cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat
    26521 gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    26581 aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa
    26641 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    26701 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    26761 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    26821 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    26881 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    26941 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    27001 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    27061 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    27121 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    27181 ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca
    27241 ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    27301 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    27361 gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg
    27421 acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta
    27481 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    27541 cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag
    27601 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgcgc
    27661 cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    27721 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    27781 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    27841 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc
    27901 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    27961 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga
    28021 accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc
    28081 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac
    28141 acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    28201 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc
    28261 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    28321 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    28381 ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg
    28441 agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    28501 ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt
    28561 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg
    28621 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    28681 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    28741 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    28801 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    28861 acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat
    28921 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    28981 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    29041 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    29101 ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg
    29161 gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    29221 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    29281 tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc
    29341 tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg
    29401 tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg
    29461 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc
    29521 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    29581 tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc
    29641 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac
    29701 taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc
    29761 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt
    29821 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg
    29881 gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    29941 gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat
    30001 ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    30061 ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    30121 acaggagtta accatgaaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    30181 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca
    30241 tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta
    30301 tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga
    30361 ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga
    30421 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    30481 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt
    30541 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg
    30601 cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc
    30661 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    30721 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    30781 tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg
    30841 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    30901 gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat
    30961 catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    31021 gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga
    31081 agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca
    31141 ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg
    31201 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    31261 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    31321 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    31381 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc
    31441 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    31501 tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg
    31561 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    31621 gggatgccgt taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag
    31681 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    31741 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    31801 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    31861 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    31921 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    31981 actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    32041 tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg
    32101 ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc
    32161 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    32221 tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    32281 taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga
    32341 ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    32401 gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc
    32461 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    32521 aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca
    32581 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    32641 gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    32701 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    32761 cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt
    32821 tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca
    32881 ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    32941 aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    33001 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    33061 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    33121 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    33181 ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt
    33241 ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg
    33301 ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    33361 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    33421 caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc
    33481 gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat
    33541 cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    33601 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac
    33661 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    33721 taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca
    33781 gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg
    33841 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    33901 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    33961 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    34021 ggccgccggg atgaattctg ctg
//
LOCUS       DADLDT020000034        33529 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000034 DADLDT020000000
VERSION     DADLDT020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33529
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.034"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="JQH22_001533"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="JQH22_001533"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFB2205900.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="JQH22_001534"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="JQH22_001534"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFB2205901.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQH22_001535"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQH22_001535"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFB2205902.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQH22_001536"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQH22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFB2205903.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQH22_001537"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQH22_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFB2205904.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="JQH22_001538"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="JQH22_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFB2205905.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="JQH22_001539"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="JQH22_001539"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFB2205906.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="JQH22_001540"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="JQH22_001540"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFB2205907.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="JQH22_001541"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="JQH22_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFB2205908.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQH22_001542"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQH22_001542"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFB2205909.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQH22_001543"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQH22_001543"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFB2205910.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="JQH22_001544"
     CDS             8212..9075
                     /locus_tag="JQH22_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HFB2205911.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="JQH22_001545"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="JQH22_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFB2205912.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="JQH22_001546"
     CDS             10411..11028
                     /locus_tag="JQH22_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFB2205913.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQH22_001547"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQH22_001547"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFB2205914.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="JQH22_001548"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="JQH22_001548"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFB2205915.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQH22_001549"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQH22_001549"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFB2205916.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="JQH22_001550"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="JQH22_001550"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFB2205917.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="JQH22_001551"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="JQH22_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFB2205918.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="JQH22_001552"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="JQH22_001552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFB2205919.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQH22_001553"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQH22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFB2205920.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="JQH22_001554"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="JQH22_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFB2205921.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="JQH22_001555"
     CDS             21859..23568
                     /locus_tag="JQH22_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFB2205922.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQH22_001556"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQH22_001556"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFB2205923.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="JQH22_001557"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="JQH22_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="JQH22_001558"
     tRNA            27625..27719
                     /locus_tag="JQH22_001558"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="JQH22_001559"
     CDS             28020..29204
                     /locus_tag="JQH22_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2205924.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="JQH22_001560"
     CDS             29616..30659
                     /locus_tag="JQH22_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFB2205925.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="JQH22_001561"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="JQH22_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="JQH22_001562"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="JQH22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="JQH22_001563"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="JQH22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8401 a         8810 c         8263 g         8055 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctac
//
LOCUS       DADLDT020000035        33395 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000035 DADLDT020000000
VERSION     DADLDT020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33395
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.035"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            529..666
                     /locus_tag="JQH22_001564"
     CDS             529..666
                     /locus_tag="JQH22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205926.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="JQH22_001565"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="JQH22_001565"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFB2205927.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /gene="virB"
                     /locus_tag="JQH22_001566"
     CDS             complement(2992..3921)
                     /gene="virB"
                     /locus_tag="JQH22_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFB2205928.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="JQH22_001567"
     CDS             complement(4138..4374)
                     /locus_tag="JQH22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFB2205929.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQH22_001568"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQH22_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFB2205930.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQH22_001569"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQH22_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFB2205931.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQH22_001570"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQH22_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFB2205932.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQH22_001571"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQH22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFB2205933.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQH22_001572"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQH22_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFB2205934.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="JQH22_001573"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="JQH22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFB2205935.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JQH22_001574"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JQH22_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFB2205936.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JQH22_001575"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JQH22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFB2205937.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="JQH22_001576"
     CDS             13562..15178
                     /locus_tag="JQH22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFB2205938.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="JQH22_001577"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="JQH22_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFB2205939.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="JQH22_001578"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="JQH22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFB2205940.1"
                     /translation="MSRFVFILLCFTPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="JQH22_001579"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="JQH22_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFB2205941.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /gene="mxiH"
                     /locus_tag="JQH22_001580"
     CDS             17140..17391
                     /gene="mxiH"
                     /locus_tag="JQH22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFB2205942.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="JQH22_001581"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="JQH22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFB2205943.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="JQH22_001582"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="JQH22_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFB2205944.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="JQH22_001583"
     CDS             18425..18952
                     /locus_tag="JQH22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFB2205945.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="JQH22_001584"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="JQH22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFB2205946.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="JQH22_001585"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="JQH22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFB2205947.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="JQH22_001586"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="JQH22_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFB2205948.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="JQH22_001587"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="JQH22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFB2205949.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="JQH22_001588"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="JQH22_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFB2205950.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="JQH22_001589"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="JQH22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFB2205951.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="JQH22_001590"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="JQH22_001590"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFB2205952.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="JQH22_001591"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="JQH22_001591"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFB2205953.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="JQH22_001592"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="JQH22_001592"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFB2205954.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28168
                     /gene="spa13"
                     /locus_tag="JQH22_001593"
                     /gene_synonym="spaM"
     CDS             27812..28168
                     /gene="spa13"
                     /locus_tag="JQH22_001593"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFB2205955.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="JQH22_001594"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="JQH22_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFB2205956.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGLFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spa33"
                     /locus_tag="JQH22_001595"
                     /gene_synonym="spaO"
     CDS             29009..29890
                     /gene="spa33"
                     /locus_tag="JQH22_001595"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFB2205957.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spa24"
                     /locus_tag="JQH22_001596"
                     /gene_synonym="spaP"
     CDS             29895..30545
                     /gene="spa24"
                     /locus_tag="JQH22_001596"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFB2205958.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="JQH22_001597"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="JQH22_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFB2205959.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spa29"
                     /locus_tag="JQH22_001598"
                     /gene_synonym="spaR"
     CDS             30820..31590
                     /gene="spa29"
                     /locus_tag="JQH22_001598"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFB2205960.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spa40"
                     /locus_tag="JQH22_001599"
                     /gene_synonym="spaS"
     CDS             31597..32625
                     /gene="spa40"
                     /locus_tag="JQH22_001599"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFB2205961.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="JQH22_001600"
     CDS             32638..32880
                     /locus_tag="JQH22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205962.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10424 a         4841 c         6680 g        11450 t
ORIGIN      
        1 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg ctgtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atataatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtggg ttttttgttt atcaccatgt
     2641 aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgtagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttcttatat ttgcatctgt aacttttaat ttgttgttta ttctatgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat ttccacctag attctctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatattatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggagctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc gttcctggaa agaatattca atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7381 ttgattggtt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattttctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaacctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcggtga tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataagt
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgcgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgatgattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggatcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gtatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttgcgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg ggagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttaccccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaacat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gcaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaatatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccgaat
    17161 gatgattgga cattgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 ggtgaactaa cattggcact agataaatta gctaaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atccaataca
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaagt taaatcggaa tttttaacaa atgaagtaat atatttattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg tgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttcagctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaactg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatatagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaataat atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
    23161 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga agtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tggggtctta taatcaatga gctatacaaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acagataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttactga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttagaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagacaa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggctc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgactcattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgcactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagaaca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 tcttttagaa aaaaatatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgacatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtacct gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc tcaaaaattt gatacaatat ctgatattgt ccgtttttct gattctggct
    30181 tgatggaata taagcaatac ttgaaaaagc atacagatct tgagttagca cgttttttcc
    30241 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaatcaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttgtt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtggggtatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat tatctattgg
    30661 tttgttacag acagtgactc aactccaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt agctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggccttct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggtat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggcgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgactgttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg gaggctgttt aaggatataa tattattttt cttggcattt
    32161 tctattcttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggac
//
LOCUS       DADLDT020000036        32634 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000036 DADLDT020000000
VERSION     DADLDT020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.036"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..956)
                     /locus_tag="JQH22_001601"
     CDS             complement(<1..956)
                     /locus_tag="JQH22_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFB2205963.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="JQH22_001602"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="JQH22_001602"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFB2205964.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="JQH22_001603"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="JQH22_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQH22_001604"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQH22_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFB2205965.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="JQH22_001605"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="JQH22_001605"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFB2205966.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="JQH22_001606"
     CDS             complement(5018..6346)
                     /locus_tag="JQH22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFB2205967.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="JQH22_001607"
     CDS             complement(6354..8702)
                     /locus_tag="JQH22_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFB2205968.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="JQH22_001608"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="JQH22_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFB2205969.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="JQH22_001609"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="JQH22_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFB2205970.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="JQH22_001610"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="JQH22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFB2205971.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="JQH22_001611"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="JQH22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFB2205972.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="JQH22_001612"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="JQH22_001612"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFB2205973.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="JQH22_001613"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="JQH22_001613"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFB2205974.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="JQH22_001614"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="JQH22_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFB2205975.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVASARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="JQH22_001615"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="JQH22_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFB2205976.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="JQH22_001616"
     CDS             18273..19172
                     /locus_tag="JQH22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFB2205977.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="JQH22_001617"
     CDS             19178..21610
                     /locus_tag="JQH22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFB2205978.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="JQH22_001618"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="JQH22_001618"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFB2205979.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="JQH22_001619"
     CDS             22723..23988
                     /locus_tag="JQH22_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFB2205980.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="JQH22_001620"
     CDS             complement(24229..25821)
                     /locus_tag="JQH22_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFB2205981.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="JQH22_001621"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="JQH22_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFB2205982.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="JQH22_001622"
     CDS             complement(27018..28136)
                     /locus_tag="JQH22_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFB2205983.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="JQH22_001623"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="JQH22_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFB2205984.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="JQH22_001624"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="JQH22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFB2205985.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="JQH22_001625"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="JQH22_001625"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFB2205986.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="JQH22_001626"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="JQH22_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFB2205987.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="JQH22_001627"
     CDS             complement(31385..31450)
                     /locus_tag="JQH22_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205988.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="JQH22_001628"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="JQH22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFB2205989.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8166 a         8701 c         7884 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggcatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DADLDT020000037        32142 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000037 DADLDT020000000
VERSION     DADLDT020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32142
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.037"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="JQH22_001629"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="JQH22_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="JQH22_001630"
     CDS             1127..1234
                     /locus_tag="JQH22_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2205990.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="JQH22_001631"
     CDS             complement(1287..2045)
                     /locus_tag="JQH22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFB2205991.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="JQH22_001632"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="JQH22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFB2205992.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="JQH22_001633"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="JQH22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFB2205993.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="JQH22_001634"
     CDS             3758..4618
                     /locus_tag="JQH22_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFB2205994.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="JQH22_001635"
     CDS             complement(4659..5195)
                     /locus_tag="JQH22_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFB2205995.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="JQH22_001636"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="JQH22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFB2205996.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="JQH22_001637"
     CDS             6173..6763
                     /locus_tag="JQH22_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFB2205997.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="JQH22_001638"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="JQH22_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8290..9106)
                     /locus_tag="JQH22_001639"
                     /pseudo
     CDS             complement(8290..9106)
                     /locus_tag="JQH22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="JQH22_001640"
     CDS             complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="JQH22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFB2205998.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9829..12090
                     /gene="katE"
                     /locus_tag="JQH22_001641"
     CDS             9829..12090
                     /gene="katE"
                     /locus_tag="JQH22_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFB2205999.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLKPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="JQH22_001642"
     CDS             complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="JQH22_001642"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFB2206000.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12908..14260)
                     /gene="celF"
                     /locus_tag="JQH22_001643"
                     /gene_synonym="chbF"
     CDS             complement(12908..14260)
                     /gene="celF"
                     /locus_tag="JQH22_001643"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFB2206001.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="JQH22_001644"
                     /pseudo
     CDS             complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="JQH22_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="JQH22_001645"
     CDS             complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="JQH22_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFB2206002.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="JQH22_001646"
     CDS             complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="JQH22_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFB2206003.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="JQH22_001647"
     CDS             complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="JQH22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFB2206004.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="JQH22_001648"
     CDS             complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="JQH22_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFB2206005.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18217..19044
                     /gene="nadE"
                     /locus_tag="JQH22_001649"
     CDS             18217..19044
                     /gene="nadE"
                     /locus_tag="JQH22_001649"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFB2206006.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19274..20161
                     /gene="cho"
                     /locus_tag="JQH22_001650"
     CDS             19274..20161
                     /gene="cho"
                     /locus_tag="JQH22_001650"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFB2206007.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20121..20696)
                     /gene="ves"
                     /locus_tag="JQH22_001651"
     CDS             complement(20121..20696)
                     /gene="ves"
                     /locus_tag="JQH22_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFB2206008.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20899..21384)
                     /gene="spy"
                     /locus_tag="JQH22_001652"
     CDS             complement(20899..21384)
                     /gene="spy"
                     /locus_tag="JQH22_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HFB2206009.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21714..22682)
                     /gene="astE"
                     /locus_tag="JQH22_001653"
     CDS             complement(21714..22682)
                     /gene="astE"
                     /locus_tag="JQH22_001653"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFB2206010.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22675..24008)
                     /gene="astB"
                     /locus_tag="JQH22_001654"
                     /pseudo
     CDS             complement(22675..24008)
                     /gene="astB"
                     /locus_tag="JQH22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24005..25483)
                     /gene="astD"
                     /locus_tag="JQH22_001655"
     CDS             complement(24005..25483)
                     /gene="astD"
                     /locus_tag="JQH22_001655"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFB2206011.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25480..26514)
                     /gene="astA"
                     /locus_tag="JQH22_001656"
     CDS             complement(25480..26514)
                     /gene="astA"
                     /locus_tag="JQH22_001656"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFB2206012.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERTI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26511..27731)
                     /gene="astC"
                     /locus_tag="JQH22_001657"
                     /pseudo
     CDS             complement(26511..27731)
                     /gene="astC"
                     /locus_tag="JQH22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28177..28983
                     /gene="xthA"
                     /locus_tag="JQH22_001658"
     CDS             28177..28983
                     /gene="xthA"
                     /locus_tag="JQH22_001658"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFB2206013.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29150..29858
                     /locus_tag="JQH22_001659"
                     /pseudo
     CDS             29150..29858
                     /locus_tag="JQH22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29863..30540
                     /gene="ydjY"
                     /locus_tag="JQH22_001660"
     CDS             29863..30540
                     /gene="ydjY"
                     /locus_tag="JQH22_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HFB2206014.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30555..31262
                     /locus_tag="JQH22_001661"
     CDS             30555..31262
                     /locus_tag="JQH22_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFB2206015.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31262..31810
                     /locus_tag="JQH22_001662"
     CDS             31262..31810
                     /locus_tag="JQH22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFB2206016.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31820..>32142
                     /locus_tag="JQH22_001663"
     CDS             31820..>32142
                     /locus_tag="JQH22_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFB2206017.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8247 a         8241 c         7612 g         8042 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttccaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaatcatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat
     8401 gtccaactta tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac
     8461 ctcatacccg agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac
     8521 accacttccc attcccggaa gatttagtgg attaaaaaga agattgatat taagcattga
     8581 tattatcgaa ggaaacttta ggagactctc attttttttg aatcgcagca aaaatgctcc
     8641 cggcaggatg tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc
     8701 tattttttat attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca
     8761 tcccaacaaa attctcatta atatctctta tccgaaactc actgccataa agattattaa
     8821 ttatcactct atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc
     8881 ctagctttga ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg
     8941 actctgctat ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca
     9001 tggtagactc aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac
     9061 cataattact ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat
     9121 tattatctta tttaaatcta agtcatttac aatataagtt taagagcgac gccacaggat
     9181 gaactatcaa aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaat
     9241 aattaaagaa aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta
     9301 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc
     9361 aaaaccggga ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
     9421 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
     9481 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
     9541 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
     9601 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
     9661 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
     9721 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
     9781 tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat
     9841 aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
     9901 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
     9961 ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa
    10021 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
    10081 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
    10141 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
    10201 cgtattgttc atgcacgcgg ctcagcagct cacggttatt tccagccata taaaagctta
    10261 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
    10321 aggttctcta ccgttcaggg tggagctggc tctgccgata ccgtgcgtga tatccgtggt
    10381 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
    10441 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
    10501 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
    10561 ctgaaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
    10621 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
    10681 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
    10741 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
    10801 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
    10861 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
    10921 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
    10981 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
    11041 gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
    11101 gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag
    11161 cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg
    11221 atcaacgata actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca
    11281 taccaagagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat
    11341 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
    11401 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
    11461 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
    11521 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
    11581 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg
    11641 attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccatgaag
    11701 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
    11761 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
    11821 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat
    11881 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
    11941 aaagcaacaa tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc
    12001 gacggtagtt ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc
    12061 attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt
    12121 gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa
    12181 ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt
    12241 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag
    12301 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
    12361 caccataaaa cgcgctactg aatccctgcg aactgcgcag attaaccggt aaatcaccag
    12421 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
    12481 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
    12541 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
    12601 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
    12661 cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc
    12721 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
    12781 tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
    12841 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
    12901 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
    12961 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
    13021 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
    13081 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
    13141 gcgtaatgcg cggatgtggc gtcgcgccat cgtgccccag cttacaggtc atttctaccg
    13201 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
    13261 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
    13321 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa
    13381 aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc
    13441 ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca
    13501 acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc
    13561 cagatacttt taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc
    13621 catttaccag cacatcctta atgaacacca tatggttgag gccaaacaaa tcgatagata
    13681 aatcatcgct gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa
    13741 tattacacac gccgataaaa cgtttaaatc cggtatgacg ataaacggct tcagtaacca
    13801 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
    13861 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt
    13921 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg
    13981 gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
    14041 aatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
    14101 gatcgaaaat aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc
    14161 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
    14221 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
    14281 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
    14341 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatatct
    14401 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
    14461 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gtttttttgc
    14521 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
    14581 cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
    14641 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actacggtac
    14701 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
    14761 catctcaaca aactcttcca gccctgtttc gcggaaattt aatgaagaaa tcactgtttc
    14821 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcaacaa agcaataagg
    14881 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg
    14941 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
    15001 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
    15061 tagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata
    15121 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
    15181 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
    15241 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
    15301 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
    15361 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gctttcgctg
    15421 cggcaaaatc gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc
    15481 cagagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
    15541 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
    15601 ccgtaccggc atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta
    15661 tcaatcgcat tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
    15721 atgccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
    15781 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
    15841 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
    15901 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
    15961 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
    16021 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
    16081 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
    16141 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
    16201 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc
    16261 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
    16321 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
    16381 gaaagaataa taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg
    16441 ctgtcgggca gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
    16501 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
    16561 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
    16621 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
    16681 gccattaaag acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga
    16741 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac
    16801 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt
    16861 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
    16921 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
    16981 tacccttaaa aaccgcaatt taaatattac ggtattgatt tatgaaataa ctctttgacg
    17041 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
    17101 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
    17161 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
    17221 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
    17281 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga gatagacatg
    17341 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
    17401 atctgtcagc cagacactcc gcaagccttg acctgcttcc atgctctggg taacttgcga
    17461 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
    17521 tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc
    17581 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
    17641 ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt
    17701 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
    17761 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
    17821 gcgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
    17881 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg
    17941 atcataagct gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
    18001 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
    18061 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
    18121 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
    18181 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
    18241 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
    18301 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
    18361 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
    18421 caggaaactc gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
    18481 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
    18541 gttaatatca agggcgcggt attggctagc gagcaggcat tgcgggaagc aggcattgaa
    18601 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
    18661 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
    18721 ggattcttca ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac
    18781 aaacgccagg gtaaacagtt actgacggca ttaggttgcc cggaacacct ttataagaaa
    18841 gccccaactg ccgatcttga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
    18901 gtgacctatg acaatatcga cgactatctg gaagggaaaa acctacctga acaggtcgcc
    18961 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaacgtt
    19021 ttcgatgatt tctggaaaaa gtaataattt gcacctattg gattgtgcga aaaagagtaa
    19081 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
    19141 atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg
    19201 tcatcaaccc gcctcttttt taatcgcctc ccgcctgtta cactggatag ataaccagca
    19261 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
    19321 aatttatgaa tatcccgaac atttacattc attccttaat gacttaccca cccgaccagg
    19381 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
    19441 tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca
    19501 atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc
    19561 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
    19621 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
    19681 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
    19741 gcagagcatc gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag
    19801 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
    19861 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgagtggt
    19921 gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata
    19981 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
    20041 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
    20101 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
    20161 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
    20221 aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta
    20281 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
    20341 actttcgcct tacagacatc caggcgcgtc ataatattga aatccatcga catctgccct
    20401 gccgtcagtt tcgcttttac cacctggtcc gccgtaaagg caaaaggctg taacggtttt
    20461 aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
    20521 actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
    20581 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
    20641 ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
    20701 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatgat tatcggcaac
    20761 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag
    20821 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
    20881 agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
    20941 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat
    21001 gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
    21061 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
    21121 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
    21181 gatttcgtgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
    21241 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc
    21301 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
    21361 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
    21421 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
    21481 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
    21541 aagaaaattt gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta
    21601 tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
    21661 taatttataa gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
    21721 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac
    21781 acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt
    21841 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
    21901 gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg
    21961 acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga
    22021 tcgttttgcc caaagggcaa cgctttgcca agttccaacg tacagaccag cgcgccaaaa
    22081 tgtctgacgc tgaaatgggt aaacgtacca ccaggctcct gatggaacac cagcgcctcc
    22141 agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
    22201 aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccaa
    22261 cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc
    22321 cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcggttcata
    22381 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc
    22441 aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt
    22501 tccacaggtg ccgtctcatt accgtggatc cccgcagaaa tcaccagcgc gccttgcggt
    22561 ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg
    22621 atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc
    22681 attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
    22741 tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt
    22801 aagacgatcg cggtagtaac gatccaccca gtcattgagc acattaaaca aggtatcgtt
    22861 catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaataccc cccgcaaccg
    22921 caggcacgcc gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc
    22981 actaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgctc
    23041 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct
    23101 gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat
    23161 cgccataaaa ccgttcaccc gcgtgccagt aactgtgact ggcgagcgaa cgcctgttgg
    23221 tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgctctgg
    23281 tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattaagc
    23341 cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta
    23401 tcattgcctt cttctcgccc gtagacaaaa agtagcatac ccggttcacc gtaatgaccg
    23461 ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc
    23521 gaatggacgc taaatttctc ttcgtcctta aaaatcgctt ttaacagcga ttcagtgacg
    23581 ggcgcttcca gcgaacggtg aaatttattg ttcaggttgg caacggtgag atgcactttg
    23641 ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa
    23701 gcggaactga cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc
    23761 tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc
    23821 gggatcacgg cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt
    23881 tttgccgcca gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca
    23941 aacgacaggc ccgcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag
    24001 gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtg gcgggcaatg
    24061 ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg
    24121 cgctggggcg atggttaccg gaagcaccaa tgccgccgaa tggggcggta ctggcagcac
    24181 cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga
    24241 atttttcccg ctcgggggaa accagaccgc aaaagagtcc gaagcgagtg ttgttcgcca
    24301 tccgaatcgc ctcatcaaaa gtatcataac gccagacgcg caaaagcggc ccgaacacct
    24361 cttcatccgg cacaccgcca acgcccgtca tttcaatgat ccctggtgtc agcaacgatg
    24421 tccctgcttg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct
    24481 gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct
    24541 gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc agactagcaa
    24601 gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt
    24661 gcccgactgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca
    24721 cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct
    24781 gaccggagag ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac
    24841 cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt
    24901 tcagcacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc
    24961 acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga
    25021 tatgtccgtt cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt
    25081 gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac
    25141 gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg
    25201 ccgcttccca gcgcggctta cccgtttctc tggcaataat cgcggttaat tcggctttat
    25261 tgctttccag caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc
    25321 gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc tgagcggcat
    25381 cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg
    25441 cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc
    25501 gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc
    25561 ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg
    25621 gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc
    25681 tgccacttcc accagccgac ttttacggat ggcgcgcacg cggtcgatgt cacactcaag
    25741 cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag
    25801 caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg
    25861 ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg
    25921 cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag
    25981 gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac
    26041 tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa
    26101 atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct
    26161 gccggtgtga tcgttactaa gaaacagcgt cggcaatgcg ttatagacat tcagctcttt
    26221 tgaggcgtga accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc
    26281 cacctcaata gcgcaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac
    26341 atagccctgc tcgcttttgg gcagttcacc ttgccaggtt ttgattgtcc tttcgatacg
    26401 cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc
    26461 aagctgcatc agcgccgaga catctgagcg ctcaacggga cggatgacca tcatgatgaa
    26521 cctcggctaa caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct
    26581 tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc
    26641 atcacgcctg ctttcgccgc ttccagagag atctgtttcg cttgcccggc gtaatcggca
    26701 ttcagtacac agccaatcaa caaacctaag ccgcgaactt cactgaacaa accatagcgg
    26761 tgattaatag tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc
    26821 atctctggtg tgttgatgag ctccagcact ttgcctgcta ccgccgaggc cagcggatta
    26881 ccgccatagg tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc
    26941 aacaacgcac cgaccgggaa accgccgccc agcgctttgg cggtggttaa cagatcgggc
    27001 gtcacgccgt agtgcatata ggcatacagc tcaccagtgc gcccgacgcc ggtttgtacc
    27061 tcatcaaaaa tcagcaaggc attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac
    27121 gcgttgctgg ctggcaccac accgccttcg ccctagatgg gctcgacaat caccgcacag
    27181 gtggcgtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata
    27241 tccgccggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta
    27301 aacagcgtgc gaccatgaaa cgcatttttg aacgccacga tgccactctt atggctgccg
    27361 tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg
    27421 gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc
    27481 agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc
    27541 agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg
    27601 aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt
    27661 ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt
    27721 ggctgagaca tagcgacctc tacagtaaat aattcgttat ttgcatgtta acaataagta
    27781 atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa
    27841 acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca
    27901 tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg
    27961 cagaagtaaa atataattct agaattgtga tcattgacga aatttactgg aaattactgc
    28021 gccattctga cgcagcgcgc accaaaagcg ggcatttttt gcgccatcat tgacatcatt
    28081 aacaaccatc gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca
    28141 tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc
    28201 aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat
    28261 gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg
    28321 aagctcggct acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg
    28381 accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag
    28441 cggcggatta ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt
    28501 tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt
    28561 tatcagaatc tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt
    28621 atgggcgata tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt
    28681 aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac
    28741 aggctgatga gctggggggt ggtcgatacc ttccgccatg ctaatccgca aacagcagat
    28801 cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc
    28861 gacctgctgc tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat
    28921 gaaattcgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc
    28981 taatttagca gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa
    29041 caaactgtca gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta
    29101 gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga
    29161 gcgtaacttt ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt
    29221 cggtttattc gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg
    29281 attgttcggt tatagcctct atattctgtt attcatcaat gccccctctt tctgttacca
    29341 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg acacaactct
    29401 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    29461 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    29521 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    29581 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    29641 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    29701 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    29761 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    29821 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    29881 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    29941 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    30001 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    30061 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    30121 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    30181 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    30241 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    30301 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    30361 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    30421 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    30481 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    30541 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    30601 tcatcatcct gttattcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    30661 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    30721 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    30781 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    30841 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    30901 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    30961 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    31021 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    31081 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    31141 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    31201 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    31261 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    31321 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    31381 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    31441 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    31501 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    31561 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    31621 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    31681 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    31741 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    31801 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    31861 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    31921 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    31981 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    32041 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    32101 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aa
//
LOCUS       DADLDT020000038        32125 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000038 DADLDT020000000
VERSION     DADLDT020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.038"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQH22_001664"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQH22_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFB2206018.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JQH22_001665"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JQH22_001665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFB2206019.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JQH22_001666"
     CDS             complement(3736..4092)
                     /locus_tag="JQH22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFB2206020.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JQH22_001667"
     CDS             complement(4096..4512)
                     /locus_tag="JQH22_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFB2206021.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JQH22_001668"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JQH22_001668"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFB2206022.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="JQH22_001669"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="JQH22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQH22_001670"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQH22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFB2206023.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQH22_001671"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQH22_001671"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFB2206024.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="JQH22_001672"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="JQH22_001672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFB2206025.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="JQH22_001673"
     CDS             10521..11504
                     /locus_tag="JQH22_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFB2206026.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQH22_001674"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQH22_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFB2206027.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQH22_001675"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQH22_001675"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFB2206028.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="JQH22_001676"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="JQH22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="JQH22_001677"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="JQH22_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFB2206029.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="JQH22_001678"
     CDS             complement(15306..15515)
                     /locus_tag="JQH22_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFB2206030.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="JQH22_001679"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="JQH22_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFB2206031.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQH22_001680"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQH22_001680"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HFB2206032.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="JQH22_001681"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="JQH22_001681"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFB2206033.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="JQH22_001682"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="JQH22_001682"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFB2206034.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQH22_001683"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQH22_001683"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFB2206035.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQH22_001684"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQH22_001684"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFB2206036.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="JQH22_001685"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="JQH22_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQH22_001686"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQH22_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFB2206037.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQH22_001687"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQH22_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFB2206038.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQH22_001688"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQH22_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFB2206039.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="JQH22_001689"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="JQH22_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFB2206040.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="JQH22_001690"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="JQH22_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFB2206041.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="JQH22_001691"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="JQH22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFB2206042.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8173 a         8246 c         7781 g         7925 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttaaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga ttaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctt
//
LOCUS       DADLDT020000039        31766 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000039 DADLDT020000000
VERSION     DADLDT020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31766
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.039"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQH22_001692"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQH22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFB2206043.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="JQH22_001693"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="JQH22_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFB2206044.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="JQH22_001694"
     CDS             2630..3391
                     /locus_tag="JQH22_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFB2206045.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="JQH22_001695"
     CDS             complement(3442..4326)
                     /locus_tag="JQH22_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFB2206046.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQH22_001696"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQH22_001696"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFB2206047.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="JQH22_001697"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="JQH22_001697"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFB2206048.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="JQH22_001698"
     CDS             6202..6474
                     /locus_tag="JQH22_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFB2206049.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="JQH22_001699"
     CDS             6844..7920
                     /locus_tag="JQH22_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFB2206050.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGVDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="JQH22_001700"
     CDS             7947..9326
                     /locus_tag="JQH22_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206051.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="JQH22_001701"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="JQH22_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="JQH22_001702"
     CDS             10409..11245
                     /locus_tag="JQH22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFB2206052.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="JQH22_001703"
     CDS             11250..12197
                     /locus_tag="JQH22_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFB2206053.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="JQH22_001704"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="JQH22_001704"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFB2206054.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="JQH22_001705"
     CDS             13324..14082
                     /locus_tag="JQH22_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HFB2206055.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="JQH22_001706"
     CDS             14200..15558
                     /locus_tag="JQH22_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206056.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JQH22_001707"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JQH22_001707"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFB2206057.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGIEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JQH22_001708"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JQH22_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFB2206058.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JQH22_001709"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JQH22_001709"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFB2206059.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="JQH22_001710"
     CDS             19503..20054
                     /locus_tag="JQH22_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFB2206060.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="JQH22_001711"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="JQH22_001711"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFB2206061.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="JQH22_001712"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="JQH22_001712"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFB2206062.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="JQH22_001713"
     CDS             23307..24347
                     /locus_tag="JQH22_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFB2206063.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="JQH22_001714"
     CDS             complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="JQH22_001714"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFB2206064.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26043..26315
                     /locus_tag="JQH22_001715"
     CDS             26043..26315
                     /locus_tag="JQH22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFB2206065.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="JQH22_001716"
     CDS             complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="JQH22_001716"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFB2206066.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26775..27395
                     /locus_tag="JQH22_001717"
     CDS             26775..27395
                     /locus_tag="JQH22_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFB2206067.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="JQH22_001718"
                     /pseudo
     CDS             complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="JQH22_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28778..29431)
                     /locus_tag="JQH22_001719"
     CDS             complement(28778..29431)
                     /locus_tag="JQH22_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFB2206068.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29431..30948)
                     /locus_tag="JQH22_001720"
                     /pseudo
     CDS             complement(29431..30948)
                     /locus_tag="JQH22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30921..31766)
                     /locus_tag="JQH22_001721"
                     /pseudo
     CDS             complement(30921..31766)
                     /locus_tag="JQH22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7958 a         7603 c         8240 g         7965 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcaaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgccgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc taggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa cctccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatctg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atatggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gcttcgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaaggggtt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatggggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcagca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctatgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccactgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatctg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
    25681 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
    25741 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
    25801 gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg tttatgtcat
    25861 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
    25921 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
    25981 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
    26041 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
    26101 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
    26161 agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg
    26221 cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa acccttagca
    26281 ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
    26341 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
    26401 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
    26461 gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt cgatgtccaa
    26521 ttcttcgttt aactcacgca ccagcacctg ctgctggctt tcatccagct cgactttacc
    26581 tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
    26641 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
    26701 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
    26761 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
    26821 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
    26881 ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg gcggcgctag
    26941 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
    27001 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt tacgcgctgg
    27061 tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt
    27121 tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac
    27181 atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg ggattaaccg
    27241 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
    27301 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
    27361 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
    27421 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
    27481 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
    27541 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
    27601 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
    27661 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
    27721 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
    27781 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
    27841 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
    27901 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
    27961 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
    28021 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
    28081 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
    28141 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
    28201 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
    28261 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
    28321 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
    28381 cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
    28441 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctagcagaga
    28501 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
    28561 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
    28621 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
    28681 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
    28741 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
    28801 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
    28861 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
    28921 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
    28981 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
    29041 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
    29101 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
    29161 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
    29221 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
    29281 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
    29341 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
    29401 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
    29461 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
    29521 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
    29581 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
    29641 tgaaaatccc accgcgaagg cagtcagcac agggcgcaac atgagtgggc atttcacgta
    29701 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
    29761 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
    29821 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
    29881 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
    29941 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
    30001 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
    30061 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
    30121 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
    30181 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
    30241 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
    30301 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
    30361 tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc
    30421 attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt
    30481 cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact gcaagggtga
    30541 ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat agtcatatag
    30601 cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat ggggaatggc
    30661 gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc atagcgccac
    30721 aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg acaccagcgt
    30781 tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc gtgtcaacga
    30841 caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa tcaccgccac
    30901 catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc ttgttccagt
    30961 gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg cggcattctt
    31021 gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac agaaggatcg
    31081 ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag gaaattggca
    31141 actaccttcg ccgcagcact ggcattcgcg ttggctggag tggtgacgaa atgcacgttg
    31201 ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc gccgctggcg
    31261 attttttgct gcgcatgtgc ggggttaaag gtcagcgaca ggcgcaatgt gccggctttc
    31321 agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg ccacaaatac
    31381 ggatgcagtg catcaagata ttgctacaag ggcgcagtga cacgggcgaa agtcgcatcg
    31441 tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa ctgttcaaga
    31501 aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg attggcttta
    31561 gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc gcggcgggcg
    31621 ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg cactgaaaaa
    31681 tctccccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt ctccgcccag
    31741 cccgtttgta ataaattggc ctcttt
//
LOCUS       DADLDT020000040        31554 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000040 DADLDT020000000
VERSION     DADLDT020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31554
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.040"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="JQH22_001722"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="JQH22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFB2206069.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="JQH22_001723"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="JQH22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="JQH22_001724"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="JQH22_001724"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFB2206070.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="JQH22_001725"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="JQH22_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFB2206071.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="JQH22_001726"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="JQH22_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HFB2206072.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="JQH22_001727"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="JQH22_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="JQH22_001728"
     CDS             7860..8363
                     /locus_tag="JQH22_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFB2206073.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="JQH22_001729"
     CDS             complement(8443..8694)
                     /locus_tag="JQH22_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HFB2206074.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="JQH22_001730"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="JQH22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFB2206075.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="JQH22_001731"
     CDS             9158..9481
                     /locus_tag="JQH22_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFB2206076.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="JQH22_001732"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="JQH22_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFB2206077.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="JQH22_001733"
     CDS             complement(10186..10425)
                     /locus_tag="JQH22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HFB2206078.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="JQH22_001734"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="JQH22_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFB2206079.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="JQH22_001735"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="JQH22_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFB2206080.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="JQH22_001736"
     tRNA            complement(12749..12835)
                     /locus_tag="JQH22_001736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="JQH22_001737"
     tRNA            complement(12848..12921)
                     /locus_tag="JQH22_001737"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="JQH22_001738"
     tRNA            complement(12976..13051)
                     /locus_tag="JQH22_001738"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="JQH22_001739"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="JQH22_001739"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFB2206081.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="JQH22_001740"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="JQH22_001740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFB2206082.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="JQH22_001741"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="JQH22_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFB2206083.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="JQH22_001742"
     CDS             16589..16765
                     /locus_tag="JQH22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206084.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="JQH22_001743"
     CDS             16752..16976
                     /locus_tag="JQH22_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HFB2206085.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="JQH22_001744"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="JQH22_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="JQH22_001745"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="JQH22_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HFB2206086.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="JQH22_001746"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="JQH22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFB2206087.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="JQH22_001747"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="JQH22_001747"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFB2206088.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="JQH22_001748"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="JQH22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2206089.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="JQH22_001749"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="JQH22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFB2206090.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="JQH22_001750"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="JQH22_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFB2206091.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="JQH22_001751"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="JQH22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFB2206092.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="JQH22_001752"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="JQH22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFB2206093.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="JQH22_001753"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="JQH22_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFB2206094.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="JQH22_001754"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="JQH22_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFB2206095.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="JQH22_001755"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="JQH22_001755"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFB2206096.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="JQH22_001756"
     CDS             complement(28474..28887)
                     /locus_tag="JQH22_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFB2206097.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="JQH22_001757"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="JQH22_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFB2206098.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="JQH22_001758"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="JQH22_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFB2206099.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="JQH22_001759"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="JQH22_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..>31554)
                     /locus_tag="JQH22_001760"
     CDS             complement(31326..>31554)
                     /locus_tag="JQH22_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2206100.1"
                     /translation="AYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7936 a         8057 c         7455 g         8106 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgttaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgct
//
LOCUS       DADLDT020000041        31023 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000041 DADLDT020000000
VERSION     DADLDT020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31023
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.041"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(435..641)
                     /locus_tag="JQH22_001761"
     CDS             complement(435..641)
                     /locus_tag="JQH22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFB2206101.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JQH22_001762"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JQH22_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFB2206102.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JQH22_001763"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JQH22_001763"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFB2206103.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JQH22_001764"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JQH22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="JQH22_001765"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="JQH22_001765"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFB2206104.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="JQH22_001766"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="JQH22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="JQH22_001767"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="JQH22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFB2206105.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="JQH22_001768"
     CDS             8989..9093
                     /locus_tag="JQH22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HFB2206106.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="JQH22_001769"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="JQH22_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="JQH22_001770"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="JQH22_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFB2206107.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="JQH22_001771"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="JQH22_001771"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HFB2206108.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="JQH22_001772"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="JQH22_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFB2206109.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="JQH22_001773"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="JQH22_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFB2206110.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="JQH22_001774"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="JQH22_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFB2206111.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="JQH22_001775"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="JQH22_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFB2206112.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="JQH22_001776"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="JQH22_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFB2206113.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="JQH22_001777"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="JQH22_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFB2206114.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="JQH22_001778"
     CDS             complement(18055..18381)
                     /locus_tag="JQH22_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206115.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="JQH22_001779"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="JQH22_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFB2206116.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="JQH22_001780"
     CDS             complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="JQH22_001780"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFB2206117.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="JQH22_001781"
     CDS             complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="JQH22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFB2206118.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24549..25349
                     /gene="nagB"
                     /locus_tag="JQH22_001782"
     CDS             24549..25349
                     /gene="nagB"
                     /locus_tag="JQH22_001782"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFB2206119.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25409..26557
                     /gene="nagA"
                     /locus_tag="JQH22_001783"
     CDS             25409..26557
                     /gene="nagA"
                     /locus_tag="JQH22_001783"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFB2206120.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26566..27786
                     /gene="nagC"
                     /locus_tag="JQH22_001784"
                     /gene_synonym="nagR"
     CDS             26566..27786
                     /gene="nagC"
                     /locus_tag="JQH22_001784"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFB2206121.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27834..28586
                     /gene="nagD"
                     /locus_tag="JQH22_001785"
     CDS             27834..28586
                     /gene="nagD"
                     /locus_tag="JQH22_001785"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFB2206122.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28983..30647
                     /gene="asnB"
                     /locus_tag="JQH22_001786"
     CDS             28983..30647
                     /gene="asnB"
                     /locus_tag="JQH22_001786"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFB2206123.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7396 a         7832 c         8076 g         7719 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat
    20521 gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc
    20581 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20641 agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20701 acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20761 cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc cttaatgacg
    20821 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    20881 tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac ctgacggctc
    20941 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctgatag
    21001 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg
    21061 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    21121 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    21181 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    21241 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21301 tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag acgacggttg
    21361 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    21421 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21481 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21541 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc
    21601 gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21661 gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21721 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21781 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    21841 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21901 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21961 ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag atcttcatcg
    22021 atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    22081 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    22141 gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg
    22201 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca
    22261 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22321 agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22381 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
    22441 tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22501 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    22561 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22621 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22681 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    22741 tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag ctgacgcagc
    22801 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22861 gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg
    22921 tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat
    22981 acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    23041 cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga tctcgtcttc
    23101 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    23161 gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt tctggctggc
    23221 ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag agaagccggc
    23281 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23341 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23401 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    23461 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23521 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    23581 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23641 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23701 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23761 ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac
    23821 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23881 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23941 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    24001 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
    24061 gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    24121 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    24181 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    24241 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg atgttctgta
    24301 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta
    24361 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24421 ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg cctgtctgcg
    24481 caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt tacttattga
    24541 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24601 gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg tttgtactgg
    24661 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24721 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24781 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24841 atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc gatgccgagt
    24901 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24961 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    25021 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    25081 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag
    25141 aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc gccgttgaag
    25201 gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa gcgatcatgg
    25261 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25321 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc cttgctcagg
    25381 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25441 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25501 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25561 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
    25621 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25681 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
    25741 gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25801 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25861 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
    25921 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25981 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    26041 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    26101 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
    26161 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
    26221 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    26281 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26341 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    26401 gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac gtctcggcac
    26461 gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa
    26521 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26581 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26641 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    26701 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26761 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26821 ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct gtttgatctc
    26881 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26941 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    27001 gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    27061 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    27121 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
    27181 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    27241 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    27301 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27361 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27421 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27481 aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc
    27541 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg tgaaatcacc
    27601 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca agcgctgaag
    27661 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    27721 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27781 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27841 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27901 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27961 actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
    28021 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
    28081 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    28141 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca
    28201 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    28261 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
    28321 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
    28381 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc ggcgataacc
    28441 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28501 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
    28561 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
    28621 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
    28681 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    28741 caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa taaaacccgc
    28801 taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt caccaatcga
    28861 caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca gtgctgcttt
    28921 gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg
    28981 ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga
    29041 aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg
    29101 ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg
    29161 cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt gaaatctaca
    29221 accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact
    29281 gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg
    29341 gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc
    29401 atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg tatgtggcct
    29461 cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct
    29521 atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg
    29581 atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg
    29641 ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt ggtctggatt
    29701 cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac
    29761 gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg
    29821 atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact
    29881 tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg
    29941 atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga
    30001 tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt
    30061 acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg
    30121 ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag
    30181 cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg
    30241 ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat
    30301 acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca
    30361 gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa
    30421 ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg
    30481 agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt
    30541 ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa aaaatggacg
    30601 atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcggtact
    30661 gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc tgatatgcag
    30721 taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta
    30781 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa
    30841 agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa tatagcgcca
    30901 cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg
    30961 acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa
    31021 aag
//
LOCUS       DADLDT020000042        30695 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000042 DADLDT020000000
VERSION     DADLDT020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30695
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.042"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..1832)
                     /gene="traD"
                     /locus_tag="JQH22_001787"
                     /pseudo
     CDS             complement(1..1832)
                     /gene="traD"
                     /locus_tag="JQH22_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            complement(1883..2620)
                     /locus_tag="JQH22_001788"
     CDS             complement(1883..2620)
                     /locus_tag="JQH22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206124.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2823..3554)
                     /locus_tag="JQH22_001789"
     CDS             complement(2823..3554)
                     /locus_tag="JQH22_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFB2206125.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3568..4077)
                     /gene="traS"
                     /locus_tag="JQH22_001790"
     CDS             complement(3568..4077)
                     /gene="traS"
                     /locus_tag="JQH22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFB2206126.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4074..6899)
                     /gene="traG"
                     /locus_tag="JQH22_001791"
     CDS             complement(4074..6899)
                     /gene="traG"
                     /locus_tag="JQH22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HFB2206127.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6896..8269)
                     /locus_tag="JQH22_001792"
     CDS             complement(6896..8269)
                     /locus_tag="JQH22_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFB2206128.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8256..8681)
                     /gene="trbF"
                     /locus_tag="JQH22_001793"
     CDS             complement(8256..8681)
                     /gene="trbF"
                     /locus_tag="JQH22_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFB2206129.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8629..8976)
                     /gene="trbJ"
                     /locus_tag="JQH22_001794"
     CDS             complement(8629..8976)
                     /gene="trbJ"
                     /locus_tag="JQH22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFB2206130.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8906..9451)
                     /gene="trbB"
                     /locus_tag="JQH22_001795"
     CDS             complement(8906..9451)
                     /gene="trbB"
                     /locus_tag="JQH22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFB2206131.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9438..9722)
                     /gene="traQ"
                     /locus_tag="JQH22_001796"
     CDS             complement(9438..9722)
                     /gene="traQ"
                     /locus_tag="JQH22_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFB2206132.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9803..10138
                     /locus_tag="JQH22_001797"
     CDS             9803..10138
                     /locus_tag="JQH22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFB2206133.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10119..10460)
                     /gene="trbA"
                     /locus_tag="JQH22_001798"
     CDS             complement(10119..10460)
                     /gene="trbA"
                     /locus_tag="JQH22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFB2206134.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10474..11217)
                     /gene="traF"
                     /locus_tag="JQH22_001799"
     CDS             complement(10474..11217)
                     /gene="traF"
                     /locus_tag="JQH22_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFB2206135.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11210..11467)
                     /gene="trbE"
                     /locus_tag="JQH22_001800"
     CDS             complement(11210..11467)
                     /gene="trbE"
                     /locus_tag="JQH22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFB2206136.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11494..13323)
                     /gene="traN"
                     /locus_tag="JQH22_001801"
     CDS             complement(11494..13323)
                     /gene="traN"
                     /locus_tag="JQH22_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFB2206137.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13634..14014)
                     /locus_tag="JQH22_001802"
     CDS             complement(13634..14014)
                     /locus_tag="JQH22_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206138.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14011..14649)
                     /gene="trbC"
                     /locus_tag="JQH22_001803"
     CDS             complement(14011..14649)
                     /gene="trbC"
                     /locus_tag="JQH22_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFB2206139.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14658..14963)
                     /locus_tag="JQH22_001804"
     CDS             complement(14658..14963)
                     /locus_tag="JQH22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206140.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(14993..15985)
                     /locus_tag="JQH22_001805"
     CDS             complement(14993..15985)
                     /locus_tag="JQH22_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFB2206141.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15982..16614)
                     /gene="traW"
                     /locus_tag="JQH22_001806"
     CDS             complement(15982..16614)
                     /gene="traW"
                     /locus_tag="JQH22_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFB2206142.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16611..16997)
                     /gene="trbI"
                     /locus_tag="JQH22_001807"
     CDS             complement(16611..16997)
                     /gene="trbI"
                     /locus_tag="JQH22_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFB2206143.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16994..19624)
                     /gene="traC"
                     /locus_tag="JQH22_001808"
     CDS             complement(16994..19624)
                     /gene="traC"
                     /locus_tag="JQH22_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFB2206144.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19750..20097)
                     /locus_tag="JQH22_001809"
     CDS             complement(19750..20097)
                     /locus_tag="JQH22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206145.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20125..20343)
                     /locus_tag="JQH22_001810"
     CDS             complement(20125..20343)
                     /locus_tag="JQH22_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206146.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20442..20915)
                     /locus_tag="JQH22_001811"
     CDS             complement(20442..20915)
                     /locus_tag="JQH22_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206147.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20908..21321)
                     /locus_tag="JQH22_001812"
     CDS             complement(20908..21321)
                     /locus_tag="JQH22_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206148.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21314..21535)
                     /gene="traR"
                     /locus_tag="JQH22_001813"
     CDS             complement(21314..21535)
                     /gene="traR"
                     /locus_tag="JQH22_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFB2206149.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21670..22185)
                     /gene="traV"
                     /locus_tag="JQH22_001814"
     CDS             complement(21670..22185)
                     /gene="traV"
                     /locus_tag="JQH22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFB2206150.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22182..22550)
                     /locus_tag="JQH22_001815"
     CDS             complement(22182..22550)
                     /locus_tag="JQH22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFB2206151.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(22489..23079)
                     /locus_tag="JQH22_001816"
     CDS             complement(22489..23079)
                     /locus_tag="JQH22_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFB2206152.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23069..24496)
                     /locus_tag="JQH22_001817"
     CDS             complement(23069..24496)
                     /locus_tag="JQH22_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFB2206153.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24496..25224)
                     /gene="traK"
                     /locus_tag="JQH22_001818"
     CDS             complement(24496..25224)
                     /gene="traK"
                     /locus_tag="JQH22_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFB2206154.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25211..25777)
                     /gene="traE"
                     /locus_tag="JQH22_001819"
     CDS             complement(25211..25777)
                     /gene="traE"
                     /locus_tag="JQH22_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFB2206155.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25799..26110)
                     /gene="traL"
                     /locus_tag="JQH22_001820"
     CDS             complement(25799..26110)
                     /gene="traL"
                     /locus_tag="JQH22_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFB2206156.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26125..26484)
                     /gene="traA"
                     /locus_tag="JQH22_001821"
     CDS             complement(26125..26484)
                     /gene="traA"
                     /locus_tag="JQH22_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFB2206157.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26518..26757)
                     /gene="traY"
                     /locus_tag="JQH22_001822"
     CDS             complement(26518..26757)
                     /gene="traY"
                     /locus_tag="JQH22_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFB2206158.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26865..27512)
                     /locus_tag="JQH22_001823"
     CDS             complement(26865..27512)
                     /locus_tag="JQH22_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFB2206159.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27704..28087)
                     /locus_tag="JQH22_001824"
     CDS             complement(27704..28087)
                     /locus_tag="JQH22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HFB2206160.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28420..29010
                     /locus_tag="JQH22_001825"
     CDS             28420..29010
                     /locus_tag="JQH22_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFB2206161.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29307..30128)
                     /locus_tag="JQH22_001826"
     CDS             complement(29307..30128)
                     /locus_tag="JQH22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFB2206162.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30239..30535)
                     /locus_tag="JQH22_001827"
     CDS             complement(30239..30535)
                     /locus_tag="JQH22_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206163.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(30559..30678)
                     /locus_tag="JQH22_001828"
     CDS             complement(30559..30678)
                     /locus_tag="JQH22_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFB2206164.1"
                     /translation="MPLAAKVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
BASE COUNT         7779 a         8131 c         6914 g         7871 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtaaacagc cagccgtttt tctgatcttc ccggacaccc
      721 cgcatccagt cacgtatggt gaagggttca ccgttatgct caatcccctg caggtaacgg
      781 atggctttca cgtagttggt cagcacagca cggatggaaa tcgccgtttt ctcaattttc
      841 tcttccacca ggttggccgc cggtgaatta cgcaggaagg tacgcagttt ttcgattttg
      901 atggaaagca gtgtgtccac cagtttgctg tagctgcggt tggggtcatt acgcatcaga
      961 tacgccgctt ccgcaaaaat ggtacgtcct gatccctgcc agaacgggtc ctctttcgtt
     1021 cccatcggga tcagcgtgtt cgcgacatta tcaaaatccg gctgtgtcag gcactccttc
     1081 cacaaatccc aggcggcaca gcgggcatcc agcggattca ggatcttatc gatggaggga
     1141 tcatagtaac ttttaacaaa ctcacacgaa cggtcataaa tcaccaccat gtcgccacgt
     1201 ttacgggcat agttggcaag acgtcgaata acctctgact tacccgtcga cactgttccg
     1261 tgcagacaga agttctggat ttcggaatcc cggataatcg gcagatcgcc aatccggata
     1321 tcggagtcct tgccgtcttt tttcagcatc cgggcaacgt ctttcggatt gtctgtcagc
     1381 tgacgaccac ctgtgacttc attttcgctc tgttgtttac cctgacgccc cagaatccag
     1441 gagacaacaa agaaggtaat caggcaaata accagtgcca caactgtagc cagaacgaat
     1501 gcggaccata gctgctctcc gcaccagatc atatatttat catgcagtac ctgagcagcg
     1561 ttcatccgga aggttttgcc gtaatactgg atctcataga ccggctggga ttttattaaa
     1621 tcccgcatgc cttccagtga cgtacaccac cagtaaatac agccgttgat aaacgtctgc
     1681 cagcttattt ttacccataa gaccagacca atgaggatcc agaaaaaaat aaacaggcag
     1741 taaagcatga tattggcgat ctggctgaac atgcggatac gcatggacgc aatctgaccg
     1801 ccctgggtca tatcctttgc gttaaaactc atgatgaaac tccgggaaaa tctggaacag
     1861 ggcagaaaag gatgatgtgg ggttaattca cagaaacaga ccaggtgtac cctgtatgcc
     1921 ggtggtgatg cccgcctgca gtcctgccgg aagagacctt ctctgtaaca atgtcagtca
     1981 ggcgcaatac ttcagaccag tcactcccat gcggaaactg gatctgaata aaaaggcgta
     2041 cccgttcacg gtgatccaga actgtgtccg gttcataaaa acggccactg tcaggagaaa
     2101 cacaggacgt tttattgatg agatcatact tatcggcagg agactgcgat gaaatcgccc
     2161 aggtgatatt ctccgcctgg tggccatcaa tacgactgct ccccggcgtc catgcaattt
     2221 cctgcgcact cgccacgatg atctccctgg aaacttcagc cagctttctg ggtgtacaga
     2281 ataccggtgc cagcacacac agggacaaca aattacagct gaccgcctcc ggtttaagca
     2341 aattgctccc ggatttttca ggaaaagata tttttgtcag tatttcagtt aattccttat
     2401 gcctgagaga attaataaag tacatgataa gcatcatgag aaacaatata acaataagcc
     2461 ctccgataac cactaaaagg tcagtatcaa tattattgct gtacagatag tctttagtgg
     2521 tcactgtatt atcctgtatc accggggggt attcagttgc aggcttctca gtaaattgat
     2581 tattctcacc aatatattca gcgatattgt ctgatgtcat aatgactttc tccggaagat
     2641 cccggcttcg ccggcaacac ttcacggtcg cacaaagaat ttgcacaacc gctcagtgtt
     2701 ttcatattta tttcggaaac gccgggaggt gacaaaattt attttgttac atcccggggt
     2761 tggacaacga gcaacgcgag gcgtcagcaa ggaggccggt cagatcagcc tcctgacgag
     2821 gttcagagaa tatttgcgat tgatttggcc agctggtctt cgagaacagg cttcgcctct
     2881 tcaaatttca ggttaacctt gttcgcattt gagaccacac gggtctggta tttatgctgg
     2941 ttacctgttt cagtactggt ctgaattttc gcaccggatg tgccctgacg cagggcggca
     3001 acattatccg ttgtcaccgt tgccttagta cgctctgcaa tctgcacatc cgtgatcatg
     3061 gtatagttca catcttccac catcgcatct gcagccatac ccaccagacc agcagcaagg
     3121 cccacaccga gtgtggcacc ggcagaattt gagttatagc cggtaatacc ggcacctaac
     3181 gctgcaccaa cagctgcgcc ttcataacca cggttcagcc atccctgaga ctcccgcaga
     3241 tccatcttat cggccttcag cacattcgcc tgaatccagt agtaggctgc atccggagaa
     3301 gtcaccacct gatatccttt tgctttcaca gcatcagcaa ttttgccctg cagcccactc
     3361 atgtctttat cagacgtgtt tttgatctgc agaaataccg tgcgttcgct ggcgggttta
     3421 agccagatgg tctcactcat ctgagtcttc acctcaaggt tacgcttctt gattgctgtg
     3481 ctcatcgcac cacaccctga aagggccaga gtggaactga ccagtgcaac catcatcatt
     3541 ttttttgttt tcatccagaa tatcctttta actaacagac tcttttttga acagacctat
     3601 tactgagact attgcagaga gattataacg atttatatct atagtataaa taaaggaaat
     3661 aattacaaac agagttattg ataaaaaccc aaataagaac gtatgagcaa aggaaactat
     3721 tgaaaaaaca atcacaaacg acaagatata agttttgttc ttatcataca taaaagatat
     3781 aactttcgac tcatctctga attttttagg gatggcaaga tacaacatca ttccattagt
     3841 aatggcaaca aaaaggataa aacccattac gccactaaaa catatatcaa atccagcatc
     3901 tcggatatca actttatctg ccagcaaacg aagaaatgtc ggccataata aaacccataa
     3961 aaacacatac acccctggtt ttatgacttg taataatggc ggaatattaa ctccctctac
     4021 agagagtaca tcctttagtt tctgtgtctc cgtttttata tcatagtgag tgatcataat
     4081 ggccccttca gtctatcctt tttatcatca accatttttt tcacagcctc ttctgatggc
     4141 tcaggtatta tcttttgact ttctcgctct tcattatatt tattattctg agataatgcc
     4201 tctgttttat gatgattttg cagttcagaa tactgccctc ttacaatatt ttcctcgcca
     4261 cggatactgt tctgagtatc tccgatattg ccacgatatt ctgtgaccat attatcaacc
     4321 tgatgtttta catcattacg gatattactg tcctgcgttc tttgctcaat aatggcctga
     4381 tgtccctgat ggtcagcaat aatatcctga ctgccaccac ccgaaggtac gctctccatt
     4441 cctttaccaa tatcgccacg ggattcccgc caggcattat caacaccagg ctgtacctgt
     4501 tcctgcacaa aaaaccaggc catcgcccga cgacgttctg caatctccgg cgaactggta
     4561 tttgtcagaa tagcttctgc atcctgcggt gcgcgtttca tcacatactg tgcaaactgt
     4621 tgcgacaggt cttcactcat ctgcccgctc atgctttcag tacgggaggc catttccgca
     4681 tattcatggc tgcgggtcat attcgtcgtg tactggtcgt aactctgttt cgctgagttc
     4741 agggctgcag acaactggtc cacccgggag tcagcattat tgtcagtatg gctgccggac
     4801 tcactgacct tgcgactggt aaagtaatcg ctggcttctt taaagtcctt actggcttta
     4861 gcgtcaatat catggcgagc atcatgggta gctctgctgc cactacttgc agaatgtgaa
     4921 tcatcatccg ttcgatcaag acttccctta aacccgcctc tgacaccaac cccaccacca
     4981 acaccaagaa ttccaggttt aatcccccat tcagcatgag catctgcata tccgccatac
     5041 gagaacttag aacctgttga tgccagttcc tgagtcgcct gctcattact gatattatgc
     5101 gccttcgcat agctttccac tgcactgcgc atacggctgg ccatcatgga gtcctgtgca
     5161 ctcatggtgc tgtcagcgcc accggtgaca gaatcactgc tcccccggtt agaaccaaac
     5221 tgactgagcg tgttccaggc actggcaata ctgctgctga aaccatgcag ggcactttct
     5281 gctctgttcg acgcctcccg ggccatttcc tgttgcgccg ctgcaatctg acgcgttgca
     5341 ttgataccga ccggtaaacg ggacatcgct ccgcttgcat ccatcaccat attaccgtca
     5401 cgggtctgtg tggcagttgc gccactgccg gtctggtaca tcatctgacc aaacgacgtg
     5461 gtgctgttgg tgctccagct gaagccgttc acgttttccg tctgcatgtt gccgtaggag
     5521 taattaccgt caaccacact gcctgctgca ctggccgtcg gactgataga ggaagaggcg
     5581 aagtggctgt acacgctgga aaaacctgcg ccaaggcctt ttaccatcat ccatgacaac
     5641 ggggggatca tggctgaaag atacccggca gtagaggcca ggtcagagta tttcagctgt
     5701 atctgagaga gttcagacag cacgaccggc gcaccattca tcttcgcata gaatgtcatg
     5761 gcactgttca gaatggcata caacaacggc caggtctgca gccacatcag ggcaaacaca
     5821 tagcctttca ggacggacag tgtcagtttg ttgaagacag ctgccagtac cagaagcggg
     5881 aaaataccaa tggcaattcc cgtcaggata gtctgtacca ttggcaggtt tcgcattgtc
     5941 acatgaccga tagagacatg cgccagacgt tgcttctcca tcgatgacgt ggtggccaga
     6001 ttcaccaggc tggcggtgtc accattacgg gcagcattac tggtgatacc ctctttcagg
     6061 gcatttattg tgacgttctg gcgcataatc tggctggcag actgactgga gccgtagaaa
     6121 taactgtaac tgtcactgac cagttgtctg aataacaggt ccgggtccgg tctgccgcca
     6181 aatatctgct gcacataata atgccaggtc ttgcctcccg tctttgtatc gagattcagc
     6241 ctgtctttca gcgtgaccga cgcatcctta caggttatga aattattatt gttgtcataa
     6301 acgccccgta gcgggctggg tcgggaaaaa atcagcgtgt aggggtcagc ggaggccatc
     6361 agatcttcca gcgtgtattt atgattcagg taaatatcac ccagcacgca gttctggacg
     6421 tagtcctgga acagattgat gatttccgga ttccgggaca ggaaatcggt gcttttcaca
     6481 atcagattcg ccccgaacag catgcctgtc ttgctgtagg tgacgctgtc cggttgggtg
     6541 aatatcatct cgtagctgac caccatcgca tgcccgatac gggtcgtaag agaaagtggc
     6601 atcgccagac cgaccggaac attatccacc cgatggactt tgaccaggtc actgttatca
     6661 ataatctgca ccgatgtgcg gacattaacc agcaggctga taagcacaaa cacggccacc
     6721 cagcccagca aatccatcac gttatgccgc tgtacccaca taaaagccac agcaacaaca
     6781 gacagggtga gcgcgatttt ttcaattgaa tcccaggtcc gggtactcat aaatgcagca
     6841 atggcgttca ggttattccg caaccactca ccaccggcaa tcacataaac ttcattcaca
     6901 gtgaattccc tccgaagtga tagttgttct ggtaacggct gagcatacgg gcggaaagct
     6961 gctgacgcat gtagttcatc tgacgttcga caaccagcag cgcatcctgc tggacctgta
     7021 cctgactctg gaaaatggca atctgttgct gtgcctgatt cagattctcc agaacattgt
     7081 ccatcacagc ctgcggatag ttcccggtgg caatcatggc ccgggcctgc tgaagcagtt
     7141 cctgaatgta ctggagcatg atgtcgtagc cgatatagtc cgtcagctgg taaacgacgc
     7201 tgttggacac accgagcatc tgcgggtcaa tcagatattt aaaaacggga atggtggtgc
     7261 tggaaataaa cccctgttcc cgggcgtcaa gcggagtgtc actcactgct ttattctgaa
     7321 tactggtgag cagttttgta atctgtgact tcagggcttt atcccgggcg atggtcacat
     7381 ttgcatccgc caccaccttc agacacttgt ccgaatcgtt gcagtggtaa atttttgccg
     7441 ttcccccctc cataatcgcc cggaggatat cgcggtcagt ggtacgcgcc ggcagggggg
     7501 taatctcgct gtcttcccca aaaatcagcg taccggtgag cgtcatgata aattctttca
     7561 actctttgtt gccgtcaaac attctgtttt ttttcagcgc ctcccatatg atattctggt
     7621 ttttcagcac ccgctccttg tccttgtcgc tggctttgtc acgaacagat gaggactgcc
     7681 ctccgaccgt gcacccctga cgagatgcgg cccagtcggc gaagatattg ctttcactgc
     7741 caatgtcctg acaaactttc tgctgagatt cctgcgtctg agggaacagc ccccccacaa
     7801 gcccctgtgc cacctgacag gaactgaggt tcatactgtt aatgtcactt gccattttct
     7861 gcaggaagtc ttttgcggtt tttatttccg gcaccgttgt ctgcagggca aggtcaaaaa
     7921 agtaaccggc agcattgctc ataatctgct taacaaaacg ctgcaactga tcgccattaa
     7981 taaaactgaa cgagccgaga taggcgtcga tgccgccaca tccggcattg atatccggca
     8041 gcgtcatgga aatcagctgg atatttttga cctgggtgcg ggcatacagg gagccaccat
     8101 aggcataccc ggcggcctgc ccctgccaga cgcccggctg tgtggtattg gaggcgaagc
     8161 ccagcttgtt aaagaactga ttcatatcgc tgttcacatc cgctgacgct gcgggtgtga
     8221 ccgtcagtaa agcggcacaa agaaccagaa gaggtttaat gcgaagcatt attgctgccc
     8281 tcccttaatg tgttctcccg gcgcatacgg cgtttcagca ggaatatccg gacaccccca
     8341 aatattattt cgcacaacgg cgacagagaa gccgcagcaa gaaaaccagc tgcaagcgca
     8401 tataacataa tcggaatgac atacatcagg gtgttccaca tataacgaaa attatttaat
     8461 tccgggtcag ctttccacga atcaataccg gcactgaaaa aatcatgcat aacgttcctt
     8521 acaatcaatc cggcagcaaa gaaaaccaca aaaagccaga agtatttttt tgcattatca
     8581 caaccacgtt caatctccct ttcaatttgt ttgattcccg gttctgtatc atttattttt
     8641 tctttcatca taagttccct ctgatatttc tgatgattca tgcagacagg tcatgcggct
     8701 gtaatatatt tcagacaccg aaatccatcc cacaattgcc cccacaaaaa acacaacaga
     8761 aacagaaaag gtaagacaca gcgaaacgac aatttccaca gggctggcgg gataaaaaag
     8821 cccctgacgg ataaagggga tattcaggct gacgattatt cccgtcgcga tgccggatat
     8881 tccggcaatg agtaacacca cctgcttatt tcgcaccttt ttcctctccg tatatctgca
     8941 gaacggtatc catccgcgcc ataaaactgg cggcatccgt tgccccctgc aaaagcggta
     9001 atgcctcaag tgtgttgacg ttgaccagga aggtggtcgg tgtggccacc gggatattcg
     9061 ggaagaaggt ctgcatcacg tccggtggca ccggtaatgc ttccggaaag gcagtatccc
     9121 cctgaccatc cagggtgtag ggaaaaacgg aaaagccgta ctgctgtgcc agctgcttca
     9181 gtaccgggtc aaactggtga cagtaagggc aatgcccctg cataaacagc accaccttcc
     9241 agtcggccag gttaacctgc ctgccgttgc tgaggcggaa ccagcgggga gccggctttt
     9301 ctgttctggc tgatgtgtct gctgccggtt gtgcaggctg cacggccatt ccctgcggat
     9361 tccagagtcg ttcaatttca tcccgggtgg aggcctgcac acctgtggcc atcatcaaca
     9421 aaatgaccgg cagtaattta gtgagagaca tgtccgccct cctgctgccc ttccatcatg
     9481 gcctgttgcc tggcttccag tgcctcacgc tcttccaggc tgacacgtgc aatccatgca
     9541 tccagcacct ggtatgcgcc gaacagcaca aggatggccg caatcaactc cgccagaaaa
     9601 aaagccatcc agggtttgct gtaaacaaga cgggcgacga tatgaaacca gacacccagg
     9661 gaaaataccc acattcccgt aatatcaagc cggggtaaag agaatctgcg tttacttatc
     9721 atgttaaact ccggttatta tttacatgag agtattcatg tctgtaatat tcgcgtttgt
     9781 gtgatattaa tcagaggtac aaatgaaaaa gttatctttt aaagaaaaac tggaaatagt
     9841 gagaaacatc ataagagagt cattatcagg taatgcggct tttgctttac tggtgtattc
     9901 attattgcac tccctcccca ttaaaacctt tgctgattta tttgtgacga tactgttatt
     9961 tatagcgatt atcgctgttg tgatatggct atttctaatc atctatattt atttttgtga
    10021 gttattcaga agtcactgga tagcagtctg gtttatcctc tggtctgtgg taatcaacct
    10081 cactattgga tttggcattt atgacaaatt agcaccagtc atttcactaa tgaaataata
    10141 aaaaaccaca tcactccaga ccatatggct gacaacataa tacaaacaaa catccttctg
    10201 aaactgtcaa acttcgtgat aaccggtatg gtactgactg ccattgaaac aaccggtacg
    10261 gcacactgat tcataaccgc accacaaaga aacaagaaat tatctggcag catacataca
    10321 cttatgacat ttactacaaa gaagacaaac gagctcagca gataaactcc tttattattt
    10381 ggtcctgtaa agaaataacc aacaaccccc acgataaaca ggctggcacc tgtaaaaaaa
    10441 cacaaatccc ctgtatgcat atataacctt tggttaaaaa ttcggtttaa aatcttcaga
    10501 aacgttcagg aactgttttg ccaggtcgtc ctgtgaaata aagccgtatg ataacgggcg
    10561 aacactgccc tgtttcgggt caaccagcat catggccggg aaatatttca caccgaggcg
    10621 ctgcgcctgc ccctggtcag tccgggaatc cggcaacagc ggattaatca cgccatccac
    10681 ggaaacggga ataacactca gaccatacgt atcccggaag ccattaatga cctgcgccag
    10741 ttgcccgtcg atggggtcct gcccccggta aaaaaacatg atgccgtagt gttcagccag
    10801 tttcgcaatg gcctgtcgct gctgcgcctg gtctgccgcc agctggttcc gtaccgtgcc
    10861 gttgtaatgg ctgtactgca ggttatagtc cagttcggga tgtgccagca tggccttttt
    10921 agcactcatg gtgaaaagcc cggcctgctg agtccagtaa ttctgcagcc ggaaatattt
    10981 aacaaaatta tccacgccgg gatacagaat ggcttcgtac agcgcccgct ttgttgccgt
    11041 ctgcagcgcg gccagtttct gcataatatc cggctcctga cggggggctg caggtacagg
    11101 tttattttct ttttccttcg gatttatttt ctcgttatac cactgccagc ctgcatcttt
    11161 ccctgacgcc ggaaaaataa agcagcagag taacagtggc agtaatgctt tattcatctt
    11221 cattcctttc atcatttttt ctttcatcat ctttctgcag gccagtcagg gcagaaaaac
    11281 aaaaatacag aataaacagg agcacaccgg agtaaaggat aacgaaagca gcagcaagta
    11341 tcccgtctgc cgtagtttct tttaccgtat cccacgacca gataattacc ggagaaatga
    11401 caatcgctgt aatcgaaagc cggataagaa aatcaacaag acgattagaa gtgaatataa
    11461 ctttcatcat ttcccccggt tattttccat accttactgt cctgcctgtt tcagctggtc
    11521 agcaatctgc tctttcactt tctgcgtcag aacgccactg tccgggattt tctggttatt
    11581 catcaggtct tcgtagaagt tagtgaaatc cagtctgtcg aactgaattt tctgcagctc
    11641 atcaaccgta atcccccgac agtcaggatg cttcgcactg ccaaacccga tacgcaactg
    11701 cccgttacgc ccctgctgct ggactatctg cgccagcttc gaatcaaact ggcagtagct
    11761 gcgctttttc tgcagacaga cacccagcac tttccgggag cagaactcgc ccacactcac
    11821 cgtcagtttg ttgtcctttg cctttcccag cgccttttct tcactgttgc acttcgccag
    11881 ccccacatcc tgaccccagc cgccatcctt acagcagtta ctgaagccgg ctgcggcctt
    11941 acggcagaat ttcgcctcac ccgtgaaagc cctgacatcc acaccattca gggcggcaac
    12001 atccttacct gcggcggcaa gcgcagccag ctgtgatact gcttcaccaa aatcactgtt
    12061 ttttccgctc tgtgccttat cgcattcacc gtcaaggcag aagacatcac caccacagac
    12121 catcactttt cctgatgttc tggactcgca ggagtacgtg gcgtactcat gcagacaggt
    12181 tccctcatcg gaataaaacg cgcactgacg tgaggccagc gtacaggccg gattatcgac
    12241 atatttctga caggtgccgt tatctgcgga ctgagtgaca tatttatccc ggtatgccca
    12301 gcatgcctgt gtgatgttcc agggtttacc ttccatcact ccggttttcg taccacccgg
    12361 ctctgaacac tcagtgcctg tcagcacgcc atccgcttta ttaaacggac aactctccac
    12421 ccactccacc cggggaaccc attctttatc acggaccttc atcctgagtt tcagcgtaaa
    12481 ggtggaagca ccactgacaa gcgattcata gactttcctg tcaccattcc cgtgcgggag
    12541 gcaattaccg ttagcagtac agccactacc ggtcagaacc tgcccctgtg tcacctgcaa
    12601 acctgtcgcc ccggacaggg ggtaagtatc cgactgcccg tttgccaggc cgacattaaa
    12661 ggttgtattc atgaaggtat aacgggagtt caggaagaaa aaagcggcgt atacagacaa
    12721 tgatgcactt tccactgtac cggttaccgg agccgtgaca ctgaacacca gtttcagccc
    12781 gttcatactg aaccggaact gtgagtgcgg tatcgtcact tcacgatatt catccgtgta
    12841 tttccagtcc ccggtgatac tggcagtccg tgtgcagtac tcctccacca tcgtgtcccg
    12901 ctcacaggta tagtttgtga attcactgcg gtttatctcc tgcgcactgc actgctgtcc
    12961 ggtatttccc acaatgctgt cagccctgtt caccacatcc ctgccggtct gaatgaacgg
    13021 tgcatccggt gacagaatgt ctttcggctt gttcatgaag gactctgtga tggtttttcc
    13081 ggtttcaccg gtcgcccatt ccgtggtgcc gtcatttttc aggccgccat ccccgccggc
    13141 agtcacgcca ccgtaatatt ttgtctcgtc cggattcgcg ttatagccgg ggatactctc
    13201 ctgtggctta aagccctgaa tactgctgct tccctgccct ttgatctggt gagcaaaatc
    13261 agaaccggcc cggtaatcac tgttgctgtc agcaaacaca gaaaagctca gaaaaaaaac
    13321 aattgcacaa agattgcacg gctttacgga ataattaaaa ttcagagatc tgaaaaaggt
    13381 atttatcttt tctttcataa cctgaaccat actggagaat gaagtgatgt catctcaact
    13441 tcagaactgt gttgattgtg gtaaagcatt aagtcgaaca gccacaacgt gcgggaattg
    13501 cagctcaacg gacccttttg gaaaaaaacg gcgcagcgat cgcttttacc agtggtttgt
    13561 agggctgttt attacggcta ttattattgc tggcggatta tggtacatgg ggcttcttga
    13621 cgtaccggga ttactactca gataacgact tcagtaacga tatcagtgtt ctgaaatcgg
    13681 cacataacga gtgacgacaa taccggttat accagtaatg aggggtgtcg ggctgaagcc
    13741 tgaaacagag ctgaaactta ctgttaaaag cccccttcac cgaccatccc catgccagac
    13801 gtggtattgc tgattcagca tcctgccgca gtcgctgctc aagaagggca gccattttta
    13861 ccggacaggc aaaggcaata taaggaggca gctttgtaac agggaaacca tgtccctgac
    13921 aggaagcata tgtgcggaag ccgtgaatgt tcatcgcggc caccagggca cggatctccg
    13981 gatcaatctg ataatacccg gcaggtaaag tcatttttgt ttatctcccg ggttattcag
    14041 caacgcccga gccacctgcc ggcagtcccc cgtggcggcc actttctcca gcgcctggcc
    14101 gacccgcagg ttacctcgga taatgtcgta tccctgactg cagaacacca ccagtgccgg
    14161 tacactgcga atgccgtact gtgaaaacag cgtcgggtcg atctgaacac catcagtcgc
    14221 gccgtctttc accagggaca gcacggcttc agcggtggtc ttcaggtcat tattcaccat
    14281 gccccgcagt gtggcgggta taccgtagtg ccgggtttca cccagcatcc gtttcagccc
    14341 ctcttcggga atggaaaacg acacaaaata cagggcaccc tgccggggtt tcccgtcctg
    14401 cgaagcctgc tgtttacgaa ccagctcatc cagaaaatgg ttatctgaac gctgaagggg
    14461 gttttccagc acctgtttct ccgcccaggc tttcagctga tggtcaggtt tctctcgcag
    14521 ttgtctgctt aaattttcct gctgcttcag gaactggcgg ttttcaggtg tgttcacatt
    14581 ttctgaagca tgaacagcaa gatgcatccc tgctgtcaga gcaaaaagtg caaatatttt
    14641 ccggttcatg atatttctca ctttctgcta tttacaggca tgaactcatc agaaaaaaga
    14701 taattgagaa aaacagccat aaacgcacat gtaaatgtca ccttcagata taatctgaca
    14761 agtgtcactt gttctgatat atctgccttc ttccattcat gaataatttc agcgagaaaa
    14821 ctgtcaggtg caaaataaaa taatataacg ggcaccatta caagaccaaa aggaaataat
    14881 tttaatgttc tgcttatcag cctgagcagc acagcactta ttttcctgag gtgctgaata
    14941 tatttactgt ccattgttgc cattttcatt tcctcttatt cattaaaatg atttacagga
    15001 atacacagtt acgtttacgc cacatcaggt agccgaagtt tttctttgtg ttgggcggat
    15061 tttttcccgt ctcccagcgc atcacactgc gcccgaacgg atggcactgc ccgctgtccg
    15121 gatacatatt caccatctga taacgccagc gttctttggg caggattggg gacggatatt
    15181 cattacagac ggcgttattt ttcccgatgg tttccataat catgccctga cggtgtagtt
    15241 tgaacgccat gcgttcactg accagcaggg aggactgcaa cggactggac tcattactca
    15301 cccagccatt gaacgggtac atacttccct gcgaaccggc acaccagaac agaacatcga
    15361 gaggcatatt aaaggcgctg gcaatggcat ctgctgcaca ggccccctgt gctatcggat
    15421 tggcaaagat gacagcttcc ggattgagaa tggtggtcag gctgctgtcc gtccaggtgg
    15481 ggtcaatttc agaaagataa gcgatatcca ggtcaccacc ttccagacag cccagcgatg
    15541 tgatgatgtt cagccagtac gtcagcgggt atttgtacca gtgaacgtga tagaacgccc
    15601 cgttcacctg cttctcatcc tttttcgccg ttccctgtgc cgttttacca aaagccggca
    15661 ggctgaagcc caggttaacc atgcatcccg gtgaccgggt gacgtccgtc agcgccatcg
    15721 gctcccagta accaatggcc agcccgatac gcctgaacag cggcggcggt gccggacaaa
    15781 tctgaatggg catcgacggg ttcgccgtgt cggggacctt accctgactg actttgacac
    15841 tgcccagcga gagcgggaaa atacagctcc agcagatatc cgtgatcggg tttacaaaac
    15901 gcccctcaca ggcagaatcc gcagacgctg cagggacatg accggcgaga aggaataaca
    15961 tcagcagcca caggcttcgc ttcattttct gccctcctct gccggaataa attccacctt
    16021 caggaagcga tcgccgggaa cggcgctcac ccgtgccggg acctgatcaa tgccgaggcg
    16081 ctggcagagc acaccgttct ggtcaaagta aacacggctg tccagagact tctgcatctc
    16141 cgggatactg ccctgcacga ggataatttt gctctccagt gtgggcggcg tctggcgttt
    16201 catccaggcc acctgcgccg gatcatcgcc attgatgaaa tacagcgtct ggttaaaagg
    16261 cacatactgc agcggattca tcacttcacc ctgacgggca aacacccgcc cttcgttatc
    16321 ccggatatcc gcagccagtc tgacggacgg gtcaaacaac cggctgccgt atttctccgt
    16381 gcgtccgata ccgggcacgg cagggggccg cagactgttg cggatcaccc gctccttaaa
    16441 ggcatccatt ttccggccca tctcaccgga ctgctccagt gccgtcaggc gctgcatgat
    16501 caccgtcagc atgtccggct cctttaccgg ccacagatcg ccccaggtgc caagatcggc
    16561 ggcggccaca ctctgccccc atatcagcag ggcaatcagc ccccggcatc tcatggttcc
    16621 gctctcattc gccgggcaat atcctgctgg atttcccggg tgatatccgg agccccctgt
    16681 accacggcag gcgacaccag aatgaccgcg tggtgtttct gctgccacgc ctgcaggctg
    16741 gcttccagtg ccgtgttaaa acgcgctgaa agggcttttg actgtgcttc tgacagttgt
    16801 ttctgactgg cgctgtcaaa aaatgcatcc acagtctgtt tcatgttgaa ggcggcagtc
    16861 accggtgcgt tcagtctgac aatgccgtaa ctgacggctg cgttcagtaa caccattgcc
    16921 aggcatcccg gcaccgtcca ccaccagtgg ctgcgtcggg gggctgtaac atcagtcgtt
    16981 ttttgcgttg tggtcatgca acactcctgt atttctcatg ttcttccagc caggcttcca
    17041 gcgaagccat ctccggccct gacttcttcc acgccagctg ccacaccgcc tcatgaatac
    17101 tcaggccctc cctgcgtttc tgctgcacaa actcaaaatc cgggccgtca gagctgtaca
    17161 tggcgcggct taacgggtcc acaaacagac ggtgccagga ggaatggttt tccacctgca
    17221 gcaggaagga actgaaccac tggtctttgg ccgcaccaaa cttgccaatc atgtcgcgct
    17281 gcagtggctg gaactgatcc gggaataact ggttatattt cgcgaactcc ttcgcactct
    17341 gtttgaggat aattttgtag gaggagttac cccatgccgc acgggccgca ctggaggcct
    17401 tgtcagagtc aaagtcgacg atgttctgtg tgatggtgat ataggcaccg gtatggcggc
    17461 gacaggtgcg gtaacctttt tgaataaact caccgacctt gcgatttttg aagtccagca
    17521 gacgccagcc ttcatcaatg acgttcagtt tcttgagagt acgcggcgtg cggtacatcc
    17581 tgttctcgat gtagataatc agggagaaca tcaccgcaac cagcagtgac ggacggtctt
    17641 ccagtccgcc cagctccagc accaccattt tcgcatcatc ccgcagggac ggctcatcgg
    17701 agttaaaata ctggccgtaa gtgccgttgg cagtgtactg gtcaagcagc acaatcattt
    17761 cgtccagacg gctgcggatg gtcggtgacc cggcatactg ctcgctgtca ctggcgtttt
    17821 tcaggaaatc caccacgtca tcaatacgtg ccctgttctc tttggccagc caggaggccc
    17881 tgaccgcctg cagcagcaga ccttcatgca cttcatccag gttaccgttg gggctggcca
    17941 tcactgacaa ctggtcacgg acacgctccg ctgactggtc aatatcggtg atgttcgcaa
    18001 acgggttaaa acgcagggtt tcaccgtcca gatatacgcc ccccatgttc tcacacaggg
    18061 acttgtagcc atcccccatg tcgaacacca cggcaaagcc cccggagtcc agcacgctgc
    18121 ggataagtgg ctgtatcagt ccggttttac cggccccgga ggtgccacag accgccatgt
    18181 tgtagttggt gttattcatc cccttaaaga agatatcgat aaacgccagc tggttacggt
    18241 aggtgggcgc cagcagacct gccggtgtca gggggttatc cgccactaac ggcatcaggt
    18301 tggccacatt aaagctctct gcgcgctgaa ccacaccggc ctctttcagc tgtttaaaca
    18361 gccctttccc ggccataaag ggcaggcagg tcaggaaatt gcgcatgtgg ttaaagcgcg
    18421 gtgaaatcag ctcaaaaccg tttttacgga agctgttcag gatgtcctgc tccacttcca
    18481 gtgccgtttc attattgtcc ttgcagaagg ctgtgatgtt gaggaagtag gacacgacag
    18541 aagactggcc ggagcccagc cgctggcgca gttcccccca ctccttcgcc tctttctcca
    18601 cggacggaaa ccatttggca tatgaggtct tcgacttctt ctccaggtcc atgtacttca
    18661 ggttggcttc gctgtgggtt ttcacctggt cttccaccac cagagtcagc gtcaggatga
    18721 acgggcagga aatggacatt tccgggttca gcagattgct gtagttgtcg gccatgttcc
    18781 acaggaaggc gatttccggg ttacgggcca gatggaaatt caggatgcgg gccgtgctgt
    18841 tcctgccgtt ctcacgcagg cccagcgtca ggtaatcagc ccggacattc aggtcaaaac
    18901 tgtcctccac acactgataa ttcagatcag aatacgggtc cagctgacgt cttttcgggt
    18961 acagagagtc cggattatgg ttaatcatct ccccgacaat ctcgatgaaa gcctgtgcat
    19021 ccactgtctg tgtggtgatt tttgcgccgt gaaaagaggc acggatgatt ttcaccagat
    19081 tttccatttc cagaatgtca gcccggcttt ttttcttcga gggagaacag tacgagataa
    19141 acacccggta atgacgcagg gtcaggggca gattcatccc ctccggcagc gggaactgtg
    19201 tcgctgccgc tttcatataa taggcacggg taatggcgtt aaaccgttca gcctgttcac
    19261 ctgaccagga gaactcacgc agcccgtatt caatcctgtc accaaccagc tgactggaca
    19321 tcagatggat acacaacgga ataccgcgcg gcagtttggt gcgcagcatg tgatcgagtg
    19381 cctccacaat ggacttattc gccccattga tgggaatggc ttccagcata aagcccatcg
    19441 tggtgtcatt catgaacaga ccggattcct ggttgtaatc acggtacggc agcagacggc
    19501 tgaactgcgg gaagctcatt tcgcccagaa cttcattggc ctttgcggat tcgtcaggca
    19561 gtttcagtgc tgtgacgagg gagttaaccg cctgagtgac ggcctcaagt gggttattac
    19621 tcatatgagt attccttagt gcgtttaaaa aaggaaaata tcagtctgtg gccgtggata
    19681 tatcacgcag tgccattcct gaaaccacag cacaaataag agatggaggc ttttaacagg
    19741 aaaccaggat taacgttcct ttactgaatg agcatcattt aatgcctgaa tttgtttttc
    19801 gcgaatctta ttgcgctcat tttctcttac ccgtttcacc tctgccattt cgtttctttt
    19861 tctgcattct gatgcgattt tatttgcctt accaagtgtt aaaggttcag actgataatt
    19921 aagtgtctcc tgaaatgctt ctgctgcaca gggagtttcc tgtaaaatct ctgtcagttg
    19981 ttctggtgtt gcaggttgtt gcgcaaggta aacgatgtaa aaaggaatac atacaatcat
    20041 agccataacg ccaacaaata taaaagccag aacagatttt aaatatatcg acttcatttc
    20101 attttccttc gaagcagtaa gcactcattg cccaccacca tttagtgttt ccatcacgct
    20161 gttgaaaggt tcatttgttt taacttcaat acactcccct gtatttccaa agcccctgcc
    20221 gccatgtgct tcgattgtcc gaagaacctg cacgcatacg tccgtatttg gcatgggtgt
    20281 aatattccag cttccgccac cgttaatatt ttttatcagc acaagaaaaa taaaggtatg
    20341 cattctttat tatctccgct tgtatattaa tcactgaata tcccttcagt taacttatga
    20401 ggtcataaaa cactgcagat tttcattcag atttactctc ttcagcaaac tttactctct
    20461 tcagcaaact tccggatggt atcctgttca cgctgacggg tggcagactc ctgcattgca
    20521 tccactttct cctgccagag acgccggata tccttttcat cccgaccagt cagtttttta
    20581 ccggacagca gacggttaag cagttcctgt ctcgcatccg gtacgtcttc agtcagacgc
    20641 ataagctgaa gcaggctctg acggctgaga accatgagtg attccggcag gagggtgaag
    20701 aagaaaataa aaaagcatac cgcaccacag caggcaagga ccgcaaacag gattttcaga
    20761 tatataccat caacgccgaa cgcagcaata atacagggaa tccatgcaat acacgcacaa
    20821 tacgccagga tgttatctat tcggttatgt ctgcgatata tctgttgatg ttcatccaga
    20881 atagaatcta ttttttcagc ttctgattta tgcatagtgt ttcctctgcg tttcctgcca
    20941 gagacgccgg atatcctgtt catcccgacc agtcagtttt ttaccggaca acaaacggtt
    21001 aagcagttcc tgtctcacat ctggtacgtc ttcagtcaga cgcataagcc ggagtaattc
    21061 ctgacgactg agtaccatgc gtgattcctc cggtacgctg taataattaa aaatacgact
    21121 gaatacccca caacagcagg caacggacag gcacagggat ttcagataca ggccagaaac
    21181 acagaatgtg gcaacaccac aaagaatagc tataactaaa aatcccttac ccagtgccat
    21241 acacaaagaa ccctgttttc tggatatccg ctcatgctca tccagaataa aatctatttt
    21301 ttcattttct gatttatgca tagtgtttcc tctgcctttc ctgatacgcc tgacattcaa
    21361 cgcacaacgt cacaccggga aatattttcc gccgggcttc cggaatagga tttccgcatg
    21421 cttcacagag ataaacagga ataccatgaa catttatttc ctggcgtatc cggtttattc
    21481 ctgtcatggt cagttgttct gtcactgaat atgcttcatc ggcttcatca ctcacggaat
    21541 attcctcccg acatgttctg acacgctacc gcccatccct tttcacgcac agggcactcg
    21601 ttttacaggc ggattttgaa tacagaaagc ccgccggcat tgccggctga tatacaactt
    21661 cagggccagt taattaatac gtggttttcc ccacgcagac ggtttaataa caaaaaacac
    21721 gctggatggc tgatgataaa catcctggtt atcaatataa ggcgctatcc ataatgctgc
    21781 cgtctgctca cccgttctta gcggacgagg aggtgttacc ggcgtaactg aactgaccgg
    21841 aacaaccgtt ttcacttccc gggcagcggt aaacagcggg cgtggtgcca gcagtttctg
    21901 ctccggatgt gctgtcaccg caggtctgct gccggacgga gtggtcgcgg tgacggtctg
    21961 caccggcatt gtccggaagt tcccttcagc caggcgcggc agtgatgccg caaccggctt
    22021 tgcttctgag gactgctcca gttttttggc cttctcattg gcctgctcca tcgtcataca
    22081 ggtatcggat gtggtggcgt tacactcaaa ttccgtattt gttcctgcgc agccggaaag
    22141 taataaggtt cccagcagag gaataaataa agaaatctgt ttcattgccc ttctcctttt
    22201 ttattactcc ccacaacagc agaaattaac tctgctgtca gatttgtttt ccctgcacag
    22261 ggatgtccgg agaccactgt atttcccgga gaagaaaata agctcatcga cattcccttc
    22321 ttcatcacac cgacagcagc catattaaat ttgtgcggca atgattcaga actgaacacc
    22381 ttcggagcag gaagaactat ttttgtggtg cagttactga gaacagcatg cataaaatca
    22441 tctgatacgt ttttatccgg cacaacgcgg gctgtaataa cattaatatt acgcatgttc
    22501 atttttatct ccgttgcttc tgtttgttcg gtcatcaggg aaataaacat ggcatttcct
    22561 gatttttccc ggcagggaag cgtcttccgt ataaacgtcc ttcagatatt catctgcggt
    22621 taccacccgg atacatttca tttttccggg cagcaccgga tatttcccgg tgatcgctgc
    22681 cagcgagaca tacatcagtg ccagtgttgc ccataatgcg gtgaccagtt gcatcagtga
    22741 ccggtcagtc tccctgacat aatctgcagc atcagccgcg cggtaatggc agatatcctg
    22801 tatttctgat ctgcctgaag aatcagagga agaaaaccag cattcactga ccgggaactg
    22861 tccggcaggt gctgtttgtc ttacgaagtt aatctcctta accagtaatt tccccggcgt
    22921 ggtgttgtcc ttccataaaa caaacagagc catcagggcc attgtggcca gtggcagaat
    22981 gacggtgtac ttcagacacc agaacagtcc tcgcagaacc cgggcgacaa cataccgggc
    23041 ggcatggcac gcctgacggg acgacatatt atttgccatc gttgcctcct ccctggctgc
    23101 cggacataaa ctgctgcgga ttcaggttgc ccagctgctt cagcatctgg tcagtattaa
    23161 agccgttaag atgactttca gctgatggcg gaacaggaac cggactttcc ggattatctt
    23221 cttccgctct gttctgcgtg cgctccagcg ccatctcttc cacggttttc agctggaagc
    23281 cgtcctggaa caccacggtc acttcgttgc ccgcaccaat cggtatcacc gggtgatact
    23341 gttcggcacg tttgatgtag tagtcactga gcgtctgtgc ggctttcgat gtgccgccac
    23401 cgatacccat tttcaggaca tcaccagccc cgtaagcggc tgtggcaccc agcccgacag
    23461 ccggctggga ggcgcgctcc attccctgac cgataccgtc aacaaatccc gcgccccatg
    23521 cccagccgag gattttgccg ttacgcatca ccacttcgcc cttgataccg tttttacccc
    23581 ggaagctgac atgcccctta atcggcatat caatagtttt gccgtccttc aggcaactga
    23641 tattgcgggt gcgtacaatg gcacgctcac tggacacatc cccccaggct tccagcccca
    23701 caaaacatcc cgttgcgtca tacgtcttgc tgttcggcat ttccaccagg ccggtgatac
    23761 gcaactgcat cggcaccgtg gattcattac cggtgactga ggcattggca tccgcccctt
    23821 caatcagcat ggctttcgca aaacttcctg acggaatgta cggcagcgat ggtccctgtt
    23881 ttccctcatt acgggtaaac accttacgct gtatccggtt aggcaccggc acagactggt
    23941 acgtcacctg tggtggtggc gtgacaccat tcccgggata aaacgccgtc ggcggtggaa
    24001 cagcaacgct gccctgcggc gggaaggata cgggggtgtt tcctggctgt ggttcgcctt
    24061 ccggcccggg cggtgaggca ggcatctgag gtacaggctc accggtcgcc gtgacgggat
    24121 tagcacccag ggcttttacc tgctctgcca gggcggcatt gtcctgtccc agcttttcaa
    24181 tacgacgctg gtcatcaccg cgctgtttgt tcaggacatc cagttcacga cggatttcct
    24241 catactgctt ctgcatctgc gctgccgtca cctgcatctc tgtggtggca tgctgacgca
    24301 ccttgtcatc aaaggtcgta tccaccacac ctgtcatatc cggcacaggc tcctgttcag
    24361 ccaccgcctc accgttaccg gacatgtcca catcagacag atacagtacc ccaccaatcg
    24421 cggaggctgc accgacaacc acaatcccca gccacaggta ctgcttgcgt ttcacaatgg
    24481 tattgatact ggccatcagt tgccctcccc gtcacggatg acatacacat ccatgcgcgc
    24541 acctgccagt aactggctgg caggctggct gaacatcacg gcacgggttc ccggctgcca
    24601 gaagtcactt tcccggatat tcagggcaga cagcgtttta ttttccacgg caaagcggac
    24661 catcttcagg tgattccccg tccatacggc atctgccact gaagacagcc ccgccggggc
    24721 ctgcagggtt tcctttgtca caggaacctg ataccagcct gcaggtaatt ttccgccacg
    24781 gacagcctgg ctgatggtca caagcaggga ttcgtaaggc gtggacgttt cccacgctcc
    24841 ggcttcttct ccggttccgc gcaggtcact gaccagctga atggtacgcc ccgcgccttc
    24901 acggggaaca gcctgaatgg aaaggttcag accacgctct gtttccagaa tgaacgtaaa
    24961 gggctttttg ttgacggtgg ccaccaccac accgccggag gcggtctgct cattattggt
    25021 cagggcacca tccagactgt tcacggcgat aatccggtca ccgggaacgg taaacagatt
    25081 cgggctggta ttgctgatgg caatactggc ctgaccaccg ttcaccattg gcaccacagt
    25141 gggggccagc gtaccgtttg cggccatcac gctgcaggaa aaaaacaggc cgccgaatat
    25201 tattgccgta ttattttttc tcatcgtctg tttccccgaa attatccagc caggtcaccc
    25261 cgttttcccg cttcagaata aggatgtaat gtttgatatc agtaaaaggt tcggagttac
    25321 caatccatgt tttccgaatt ccacggattt ccacacgacc atactgaggc cagacacgga
    25381 cactggtctg gaaaaaagca gaattcacgt tatctttttt aatgagtttc gcttcttcag
    25441 ccagaataac tttcatctgg ttctgtgcac ccgggcggat atattgcaga agcgcctgat
    25501 gtgaagcatc gacgatttcc ggtgagacat tcagacggag ggcaataaat gacagcgcca
    25561 tctgctgcaa ataagaggca tcggcactgt tctgtgacac ggcaaagggc acattaaatc
    25621 ccattggggt cacggcagtg cgttgctcat tctgaagacg gtagttattc accccctgaa
    25681 tgaggttaac agagaggctg agaacaataa gcactgacat aaatataaag gcgatggcca
    25741 ttacacgact ggtacttaaa cgggcaccgt gttccatata acaatcctgg tatcagttct
    25801 atttaatcca ctgccggaaa cacgaatcgg gaacattatg aaaaataccg cgcagcaggg
    25861 ctgttggcat ataccagtaa atcaggtcac gtaaccagga actgccccgc ccttttttca
    25921 gttttttaat cccgaaataa accagaaccg ctgcaccaat accgaacaga tatttcgatg
    25981 ttgtgatacc ccagccaaca cagattgctg cggggatcag ttcatccagt ggcaggccaa
    26041 accagcggct ctggttggtc agtgtttccg ggaaacgata tttcttaagt ttattctcgt
    26101 ctcccgacat cgttttattt cctgtcagag gccaacgacg ctcataccca cagcaataaa
    26161 tacagagatg atggcaaagc cgaccaggaa cttgacgttt ttggtcatca tgtacatgac
    26221 cgcaccgacc agcacctcag ccagaacaac ccatttaacg atactggagt ccttaccgaa
    26281 ggtcgcctta accgtatcgt tacctttagc catcaggtcg cctttaccag cagccatagc
    26341 taactcaggg aacaacatca tcagaacagc aaccgggatc actgcacgaa ccaggcgaag
    26401 catattcaga cgagtgaatt tagaaaaaaa cgatttattt tcaacgggcg cagaagcacc
    26461 ctgaacactt aaaacagtat tcatatgatt cctctgatta atattgacag atgaaaacta
    26521 aagttcttta aatgttgact cgttctcttc gatcacctcc ctgaatattt cttcattata
    26581 aaaatctgga taacgttgca aatgatcctg caacctcacc aatgcttcgt ttgttttcgt
    26641 tcttccacta cgctctctgg ctccaagtaa taatttattc gtttcctcat caagggtaag
    26701 taaaactttt ctacttggcg cagatcttaa agctccttta atcaccctcc ctcccataaa
    26761 taaaagagat ttagtcaaaa tattcacata agacgcacac aaaaataaac aacaaaatag
    26821 taaattacgt taacaaaaac cggcactttt agtgccggga atgcttacat ttctttacag
    26881 tgaaggctgt taatattact agcgaagtat tgatatagat aaaataacac atcagagatt
    26941 atagcatatc gcaaccactc atccctgtct gagaaaccat agtgtttttt aaaattcata
    27001 aatatttttt tagattggtc aacagagata tccattctct gagaagaata ctcatgacta
    27061 taacccaaaa gatacggtat tatcttatta aagcgatatt tgtcaggttc acaaccaaac
    27121 tccttgccct ttataaaaaa gccactaact acgtatcttt caaagaatct ccagacaaac
    27181 aaattcaagt tattcacttc cgtactttca accaccacat cccatgatac atcattcaat
    27241 attacaccat taataatact cactccattc gaggacgaga aagcatcaac ttcagcagta
    27301 tgaaataaaa tttgcgtatc aatatcaata ccagaaaacc atatttcaga atcactattc
    27361 tgacacttta ttaagtgtaa aaaaacagag tttgagtcta tgaattttcc tgactctgtg
    27421 cggacacaga ctggatattt tgcggataaa ataatttccg gcaggcctga ttgacgtatg
    27481 agtgctcttt ctctagtatc catcgcacac ataggaacct cctcaaagga ttctatggac
    27541 agtcgatgca gggagggaca agctccctgc atcggcgatt ttcaagttca aattttaacg
    27601 tggcattaac cacgtaatta atatactaat tattctgatt attgtcatca gtttgaacca
    27661 ttaaaaagtc tgatatttga acaaagtcaa aatttatttc cctttattca tcatcatttt
    27721 ttggaaagaa cctttccatc tcagccgaca ccttctctct gatatcttca accatattgg
    27781 catattcaaa ctttggattt cctgagacat gaggactgag agactcaata cccaaaattt
    27841 tcgctactga tgactgtgtt tttacaacgc attcaagaag tagtttatta aactcggcct
    27901 gattaaatgc agactctttg cgctccatct gagcctcata tacacgaaga cccagttcaa
    27961 gaagcattga agccgttgct gaaaaactga catctttttc tctcgccccc tcctgtcgac
    28021 gtttttcaat aatcgcgttt attttttcat aagcatcatt gctgatatac agattcacct
    28081 tagccattag atattacctt ttttaaacat aaaacaaagt taatttcgaa acactagaat
    28141 tcatagtgca aacagggacg caccgctagc agcgcccctg acggtatcct ataaaaaaac
    28201 acaccgcgcc gctagcagca cccctaatat aaaataatgt tttttataaa aatagtcagt
    28261 accaccccta caaagcggtg tcggcgcgtt gctgtagctg cgttaacgac gctgctttaa
    28321 aaaaatcaga tttaaacaat ataaatccac aaatacaact caatgatatt aaagataaat
    28381 cagcaaaaac ttgtttttgc gtagtgtgtg gtgcttttgg tggtgagaac caccaacctg
    28441 ttgagccttt ttgtggagtg ggttaaatta tttacggata aagtcaccag aggtggaaaa
    28501 atgaaaaaat ggatgttagc aatctgcctg atgtttataa atgggatctg cgaagccgcc
    28561 gattgctttg atcttgcagg tcgggattac aaaatagacc cggatttact gagagcgata
    28621 tcatggaaag aatcccgtta ccgggttaat gccatcggta ttaatccggt aacgggatat
    28681 ggcagcggac tgatgcaggt agattcccag cattttaacg aactggcccg ctatggaatt
    28741 aagccggaac atctgacaac agatccctgt atgaacattt ataccggtgc ttattatctg
    28801 gcaatagcct ttaaaaaatg gggcgtctcc tgggaggccg ttggtgcata caatgccgga
    28861 ttcaggaaga ccgaacgcca gaaccagaga cgtcttgcct acgcatcaga ggtttaccgg
    28921 atttatacct ggataaagag cagtaaaggc atccgggttc cgaccacgaa gaaatcactt
    28981 tcccaaatta acagtgtgca gaaaaattaa tattgtatac attgtcaaat accggttatg
    29041 ccgcgacagc ggcatggaag gccacacaca aaaatatcat atgagtgata atgctgactg
    29101 gccgaagccc cgcaaagcgg gatcccatct tatatatatc cggtaactgc gcagcctcct
    29161 gccagaccat ttttcgcggg cacaatattt acagactacc gaaaagccag ttgatgaaat
    29221 tgaggcatat cagacgacac agcaatgccg caggatgata atataagtaa agccccgaaa
    29281 aatttttcgg ggctttactt atattatcat gacttccact ccctgaactg ttcggcaatc
    29341 atccataatg ctttgttgat tcggatatca ccgtcaatgc ctgtaatggc tcttgttctg
    29401 gtatttttac cggaggcact tcgccccgat aatccacctt ttatcatatt ctcctgaacc
    29461 cgctgccagg ttgtccagag atcgttctgt ttatcctccc aacggcgggg agtaattatt
    29521 tgttctggcg tcactggcgt tttattttca tcttcgtacc tggccatcaa tgcagctctg
    29581 ccaaataaat gttgttcgtc actgttaaga tgtatctctt tcattgtctc catattttca
    29641 gtgactttat caaagacacc gagcacttca tacgctccct caataacctg cccgacaata
    29701 tcacctttat gtggaacgcg gatttcgcca aaattattcc cacacaccag gccatttgtg
    29761 cagacaaaac gaaaaattcc tgggatcatc tgataactgg atgaaccatc atgtgaatta
    29821 agcaggataa tttcaggaac ttctttcccg ttaatatgcc cttccctgcg aagacgtaac
    29881 atatgtttac tgtattcgcg gcgtcccaaa tcacgaaccc gactctgaca ggcaaagaat
    29941 ggctggaaac cttcatcacg taacttattg atgatattga tggttgggat atatgtataa
    30001 cgctcactcc gggattcatg tttatcacca gaaaagacag atggaacaaa ctgcattaat
    30061 tcgtcatccg ttaacgggcg ctcacggcga atagaattat atcgaccaaa acgggaagct
    30121 aatcgcataa cagtaactcc tttatgaagg cataaaatta ataaaagttt tttcgcttaa
    30181 tatcatcccc cctcggttat cgttaacaaa aagacaacaa ttccgagggg aaggaccatc
    30241 aaatcaggtc agttcgggac tgaccaacct gccgagtgaa aagcagtaac agcgcgatca
    30301 actcatcagc gttttttctg acaagaggag tatgattctc caggctatcc agtaagtcat
    30361 gcagctcatt attgacctgg tggagcacct taaagagtgc accagaatcc cggcagctct
    30421 gatatgcaag atttgcctga acagcacgat tctgcatgat cccgatttgt gcgattgcgt
    30481 ctttctgctg atcgcctgtt ggctgataag tggtgttaaa agaaaatgcg ctcataattc
    30541 ctcctgtatc tgatgttttc agcccccatc atctgttgag tttcgggagc cgcggttttg
    30601 acgttttgcg gggacctgcc ttttgttccc tccggtattc accatcacct gtttgagaac
    30661 cttcgccgca aggggcatca ctgccacggc ctgac
//
LOCUS       DADLDT020000043        30028 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000043 DADLDT020000000
VERSION     DADLDT020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30028
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.043"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            262..645
                     /gene="secE"
                     /locus_tag="JQH22_001829"
     CDS             262..645
                     /gene="secE"
                     /locus_tag="JQH22_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFB2206165.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            647..1192
                     /gene="nusG"
                     /locus_tag="JQH22_001830"
     CDS             647..1192
                     /gene="nusG"
                     /locus_tag="JQH22_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFB2206166.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1351..1779
                     /gene="rplK"
                     /locus_tag="JQH22_001831"
     CDS             1351..1779
                     /gene="rplK"
                     /locus_tag="JQH22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFB2206167.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1783..2487
                     /gene="rplA"
                     /locus_tag="JQH22_001832"
     CDS             1783..2487
                     /gene="rplA"
                     /locus_tag="JQH22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFB2206168.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2900..3397
                     /gene="rplJ"
                     /locus_tag="JQH22_001833"
     CDS             2900..3397
                     /gene="rplJ"
                     /locus_tag="JQH22_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFB2206169.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3464..3829
                     /gene="rplL"
                     /locus_tag="JQH22_001834"
     CDS             3464..3829
                     /gene="rplL"
                     /locus_tag="JQH22_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFB2206170.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4149..8177
                     /gene="rpoB"
                     /locus_tag="JQH22_001835"
     CDS             4149..8177
                     /gene="rpoB"
                     /locus_tag="JQH22_001835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFB2206171.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8254..12477
                     /gene="rpoC"
                     /locus_tag="JQH22_001836"
     CDS             8254..12477
                     /gene="rpoC"
                     /locus_tag="JQH22_001836"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFB2206172.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12719..13852)
                     /gene="thiH"
                     /locus_tag="JQH22_001837"
     CDS             complement(12719..13852)
                     /gene="thiH"
                     /locus_tag="JQH22_001837"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFB2206173.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13849..14619)
                     /gene="thiG"
                     /locus_tag="JQH22_001838"
     CDS             complement(13849..14619)
                     /gene="thiG"
                     /locus_tag="JQH22_001838"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFB2206174.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14621..14821)
                     /gene="thiS"
                     /locus_tag="JQH22_001839"
     CDS             complement(14621..14821)
                     /gene="thiS"
                     /locus_tag="JQH22_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFB2206175.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14805..15560)
                     /gene="thiF"
                     /locus_tag="JQH22_001840"
     CDS             complement(14805..15560)
                     /gene="thiF"
                     /locus_tag="JQH22_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFB2206176.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15553..16188)
                     /gene="thiE"
                     /locus_tag="JQH22_001841"
     CDS             complement(15553..16188)
                     /gene="thiE"
                     /locus_tag="JQH22_001841"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFB2206177.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16188..18083)
                     /gene="thiC"
                     /locus_tag="JQH22_001842"
     CDS             complement(16188..18083)
                     /gene="thiC"
                     /locus_tag="JQH22_001842"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFB2206178.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18164..18264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18317..18793)
                     /gene="rsd"
                     /locus_tag="JQH22_001843"
     CDS             complement(18317..18793)
                     /gene="rsd"
                     /locus_tag="JQH22_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFB2206179.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18888..19661
                     /gene="nudC"
                     /locus_tag="JQH22_001844"
     CDS             18888..19661
                     /gene="nudC"
                     /locus_tag="JQH22_001844"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFB2206180.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19701..20765
                     /gene="hemE"
                     /locus_tag="JQH22_001845"
     CDS             19701..20765
                     /gene="hemE"
                     /locus_tag="JQH22_001845"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFB2206181.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20922..21593
                     /gene="nfi"
                     /locus_tag="JQH22_001846"
     CDS             20922..21593
                     /gene="nfi"
                     /locus_tag="JQH22_001846"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFB2206182.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21636..22226
                     /locus_tag="JQH22_001847"
     CDS             21636..22226
                     /locus_tag="JQH22_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFB2206183.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22413..22685
                     /gene="hupA"
                     /locus_tag="JQH22_001848"
     CDS             22413..22685
                     /gene="hupA"
                     /locus_tag="JQH22_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HFB2206184.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22698..23393
                     /locus_tag="JQH22_001849"
     CDS             22698..23393
                     /locus_tag="JQH22_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFB2206185.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23395..23820)
                     /gene="zraP"
                     /locus_tag="JQH22_001850"
     CDS             complement(23395..23820)
                     /gene="zraP"
                     /locus_tag="JQH22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFB2206186.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24058..25434
                     /gene="zraS"
                     /locus_tag="JQH22_001851"
     CDS             24058..25434
                     /gene="zraS"
                     /locus_tag="JQH22_001851"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFB2206187.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25431..26756
                     /gene="zraR"
                     /locus_tag="JQH22_001852"
     CDS             25431..26756
                     /gene="zraR"
                     /locus_tag="JQH22_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFB2206188.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVMLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26753..28042)
                     /gene="purD"
                     /locus_tag="JQH22_001853"
     CDS             complement(26753..28042)
                     /gene="purD"
                     /locus_tag="JQH22_001853"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFB2206189.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28054..29643)
                     /gene="purH"
                     /locus_tag="JQH22_001854"
     CDS             complement(28054..29643)
                     /gene="purH"
                     /locus_tag="JQH22_001854"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFB2206190.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7031 a         7825 c         8263 g         6909 t
ORIGIN      
        1 gcgcgggcgt tgtagcaaaa gttctgagct aattgccgat aacatttgac gcaatgcgca
       61 ctaaaagggc atcatttgat gccctttttg cacgctttcg taccagaacc tggctcatca
      121 gtgattttct ttgtcataat cattgctgag acaggctctg ttgagggcgt ataatccgaa
      181 aagctaatac gcgtttcgat ttggtttgcc tcgcgatcgc ggggtgaaaa tgtttgtaga
      241 aaacttctga caggttggtt tatgagtgcg aataccgaag ctcaaggaag cgggcgcggc
      301 ctggaagcga tgaagtgggt cgttgtggtg gcattgctcc tggcggcgat tgtcggcaac
      361 tacctttatc gcgacattat gctgccgctg cgtgcgctgg ccgtagtaat tctgattgct
      421 gcagcgggtg gtgtcgcgct gttaacgaca aaaggtaaag ctaccattgc ttttgcccgt
      481 gaagcgcgta ccgaagtccg taaggtcatt tggccgattc gccaggaaac attgcacacc
      541 acgctgattg tggctgcggt taccgcagta atgtcactga tcctgtgggg actggatggt
      601 attctggttc gcctggtatc ctttatcact ggcctgaggt tctgagatgt ctgaagctcc
      661 taaaaagcgc tggtacgtcg ttcaggcgtt ttccggtttt gaaggccgcg tagcaacgtc
      721 gctgcgtgag catatcaaat tacacaacat ggaagatttg tttggtgaag tcatggtacc
      781 aaccgaagaa gtggttgaaa tccgtggcgg tcagcgtcgc aaaagcgaac gtaaattctt
      841 ccctggctac gtcctcgttc agatggtgat gaacgacgcg agctggcact tggtgcgcag
      901 cgtaccgcgt gtgatgggct tcatcggcgg tacttccgat cgtcctgcgc caatcagcga
      961 taaagaagtc gatgcgatta tgaaccgcct gcagcaggtt ggtgataagc cgcgtccgaa
     1021 aacgctgttt gaaccgggtg aaatggtccg tgttaatgat ggtccgttcg ctgacttcaa
     1081 cggtgttgtt gaagaagtgg attacgagaa atctcgtctg aaagtgtctg tttctatctt
     1141 cggtcgtgcg accccggtag agctggactt cagccaggtt gaaaaagcct aacccagcga
     1201 tcaaaaaagc ggcgatttaa tcgttgcaca aggcgtgaga ttggaataca atttcgcgcc
     1261 ttttgttttt atgggccttg cccgtaaaac gattttttat atcacgggga gcctctcaga
     1321 ggcgttatta cccaacttga ggaatttata atggctaaga aagtacaagc ctatgtcaag
     1381 ctgcaggttg cagctggtat ggctaacccg agtccgccag taggtccggc tctgggtcag
     1441 cagggcgtaa acatcatgga attctgcaaa gcgttcaacg caaaaactga ttccatcgaa
     1501 aaaggtctgc cgattccggt agtaatcacc gtttacgctg accgttcttt cactttcgtt
     1561 accaagaccc cgccggcagc agttctgctg aaaaaagcgg caggtatcaa gtctggttcc
     1621 ggtaagccga acaaagacaa agtgggtaaa atttcccgcg ctcagctgca ggaaatcgcg
     1681 cagaccaaag ctgccgacat gactggtgcc gacattgaag cgatgactcg ctccatcgaa
     1741 ggtactgcac gttccatggg cctggtagtg gaggactaag aaatggctaa actgaccaag
     1801 cgcatgcgtg ttatccgtga gaaagttgat gcaaccaaac agtacgacat caacgaagct
     1861 atcgcactgc tgaaagagct ggcgactgct aaattcgtag aaagcgtgga cgtagctgtt
     1921 aacctcggca tcgacgctcg taaatctgac cagaacgtac gtggtgcaac tgtactgccg
     1981 cacggtactg gccgttccgt tcgcgtagcc gtatttaccc aaggtgcaaa cgctgaagct
     2041 gctaaagctg caggcgcaga actggtaggt atggaagatc tggctgacca gatcaagaaa
     2101 ggcgaaatga actttgacgt tgttattgct tctccggatg caatgcgcgt tgttggccag
     2161 ctgggccagg ttctgggtcc gcgtggcctg atgccaaacc cgaaagtggg tactgtaaca
     2221 ccgaacgttg ctgaagcggt taaaaacgct aaagctggcc aggttcgtta ccgtaacgac
     2281 aaaaacggca tcatccacac caccatcggt aaagtggact ttgacgctga caaactgaaa
     2341 gaaaacctgg aagctctgct ggttgcgctg aaaaaagcaa aaccgactca ggcgaaaggc
     2401 gtgtacatca agaaagttag catctccacc accatgggtg caggtgttgc agttgaccag
     2461 gctggcctga gcgcttctgt aaactaatgc ctttacgtgg gcggtgattt tgtctacaat
     2521 cttaccccca cgtataatgc ttaatgcaga cgtatatccg agatattcgg gttgtggcaa
     2581 ggcggcaact gagtgagtcg ccaagagcat agctaactat gtgactggtg cgaatgaagg
     2641 aagccaacgc cgccacaagt tgaatagcga cggatagaaa agatttgttc gttggagcct
     2701 ggcctatcca ggcctccgtc gaagaccgca ggagtttcgc aagaaactta atcccctgcg
     2761 tagacggtga cagaacgcta agattattct tttatattct ggcttgtttc tgctcaccgt
     2821 aattaagacg ctctctccgt ttggaggagt gaagtgagtt ccagagattt tctctggcaa
     2881 acatccagga gcaaagctaa tggctttaaa tcttcaagac aaacaagcga ttgttgctga
     2941 agtcagcgaa gtagccaaag gcgcgctgtc tgcagtagtt gcggattccc gtggcgtaac
     3001 tgtagataaa atgactgaac tgcgtaaagc aggtcgcgaa gctggcgtat acatgcgtgt
     3061 tgttcgtaac accctgctgc gccgtgctgt tgaaggtact ccgttcgagt gcctgaaaga
     3121 cgcgtttgtt ggtccgaccc tgattgcata ctctatggaa cacccgggcg ctgctgctcg
     3181 tctgttcaaa gagttcgcga aagcgaatgc aaaatttgag gtcaaagccg ctgcctttga
     3241 aggtgagctg atcccggcgt ctcagatcga ccgcctggca actctgccga cctacgaaga
     3301 agcaattgca cgcctgatgg caaccatgaa agaagcttcg gctggcaaac tggttcgtac
     3361 tctggctgct gtacgcgatg cgaaagaagc tgcttaatcg cagttatctt tttaacgcat
     3421 tcgcttacgt ataaacttat tctgatattc aggaacaatt taaatgtcta tcactaaaga
     3481 tcaaatcatt gaagcagttg cagctatgtc tgtaatggac gttgtagaac tgatctctgc
     3541 aatggaagaa aaattcggtg tttccgctgc tgctgctgta gctgtagctg ctggcccggt
     3601 tgaagctgct gaagaaaaaa ctgaattcga cgtaattctg aaagctgctg gcgctaacaa
     3661 agttgctgtt atcaaagcag tacgtggcgc aactggcctg ggtctgaaag aagctaaaga
     3721 cttggtagaa tctgcaccgg ctgctctgaa agaaggcgtg agcaaagacg acgcagaagc
     3781 actgaaaaaa gctctggaag aagctggcgc tgaagttgaa gttaaataag ccaacccttc
     3841 cggttgcagc ctgagaaatc aggctgatgg ctggtgactt tttagtcacc agcctttttg
     3901 cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct ttcaatgctt
     3961 gtttctatcg acgacttaat atactgcgac aggacgtccg ttctgtgtaa atcgcaatga
     4021 aatggtttaa gcgtgatagc aacaggcatt gcggaaagtg ttccattttc cggtcaacaa
     4081 aatagtgttg cacaaactgt ccgctcaatg gacagatggg tcgacttgtc agcgagctga
     4141 ggaaccctat ggtttactcc tataccgaga aaaaacgtat tcgtaaggat tttggtaaac
     4201 gtccacaagt tctggatgta ccttatctcc tttctatcca gcttgactcg tttcagaaat
     4261 ttatcgagca agatcctgaa gggcagtatg gtctggaagc tgctttccgt tccgtattcc
     4321 cgattcagag ctacagcggt aattccgagc tgcaatacgt cagctaccgc cttggcgaac
     4381 cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac ctattccgca ccgctgcgcg
     4441 ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga aggcaccgta aaagacatta
     4501 aagaacaaga agtctacatg ggcgaaattc cgctcatgac agacaacggt acctttgtta
     4561 tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg tagtccgggc gtcttctttg
     4621 actccgacaa aggtaaaacc cactcttcgg gtaaagtgct gtataacgcg cgcatcatcc
     4681 cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa ggacaacctg ttcgtacgta
     4741 tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg cgccctgaac tacaccacag
     4801 agcagatcct cgacctgttc tttgaaaaag ttatctttga aatccgtgat aacaagctgc
     4861 agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc atcttttgac atcgaagcta
     4921 acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc gcgccacatt cgccagctgg
     4981 aaaaagacga cgtcaaactg atcgaagtcc cggttgagta catcgcaggt aaagtggttg
     5041 ctaaagacta tattgatgag tctaccggcg agctgatctg cgcagcgaac atggagctga
     5101 gcctggatct gctggctaag ctgagccagt ctggtcacaa gcgtatcgaa acgctgttca
     5161 ccaacgatct ggatcacggc ccatatatct ctgaaacctt acgtgtcgac ccaactaacg
     5221 accgtctgag cgcactggta gaaatctacc gcatgatgcg ccctggcgag ccgccgactc
     5281 gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc cgaagaccgt tatgacttgt
     5341 ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg cgaagaaatc gaaggttccg
     5401 gtattctgag caaagacgac atcattgatg ttatgaaaaa gctcatcgat atccgtaacg
     5461 gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg tcgtatccgt tccgttggcg
     5521 aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt agagcgtgcg gtgaaagagc
     5581 gtctgtctct gggcgatctg gataccctga tgcctcagga tatgatcaac gccaagccga
     5641 tttccgcagc agtgaaagag ttcttcggtt ccagccagct gtctcagttt atggaccaga
     5701 acaacccgct gtctgagatt acgcacaaac gtcgtatctc cgcactcggc ccaggcggtc
     5761 tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca cccgactcac tacggtcgcg
     5821 tatgtccaat cgaaacccct gaaggtccga acatcggtct gatcaactct ctgtccgtgt
     5881 acgcacagac taacgaatac ggcttccttg agactccgta tcgtaaagtg accgacggtg
     5941 ttgtaactga cgaaattcac tacctgtctg ctatcgaaga aggcaactac gttatcgccc
     6001 aggcgaactc caacctggat gaagaaggcc acttcgtaga agacctggta acttgccgta
     6061 gcaaaggcga atccagcttg ttcagccgcg accaggttga ctacatggac gtatccaccc
     6121 agcaggtggt atccgtcggt gcgtccctga tcccgttcct ggaacacgat gacgccaacc
     6181 gtgcattgat gggtgcgaat atgcaacgtc aggccgttcc gactctgcgc gctgataagc
     6241 cgctggttgg tactggtatg gaacgtgctg ttgccgttga ctccggtgta actgcggtag
     6301 ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg tatcgttatc aaagttaacg
     6361 aagacgagat gtatccgggt gaagcaggta tcgacatcta caacctgacc aaatacaccc
     6421 gttctaacca gaacacctgt atcaaccaga tgccgtgtgt gtctctgggt gaaccggttg
     6481 aacgtggcga cgtgctggca gacggtccgt ccaccgacct cggtgaactg gcgcttggtc
     6541 agaacatgcg cgtagcgttc atgccgtgga atggttacaa cttcgaagac tccatcctcg
     6601 tatccgagcg tgttgttcag gaagaccgtt tcaccaccat ccacattcag gaactggcgt
     6661 gtgtgtcccg tgacaccaag ctgggtccgg aagagatcac cgctgacatc ccgaacgtgg
     6721 gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt ttacattggt gcggaagtga
     6781 ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg tgaaactcag ctgaccccag
     6841 aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc tgacgttaaa gactcttctc
     6901 tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt tcaggtcttt actcgcgatg
     6961 gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat gcagctcaaa caggcgaaga
     7021 aagacctgtc tgaagaactg cagatcctcg aagcgggtct gttcagccgt atccgtgctg
     7081 tgctggtagc cggtggcgtt gaagctgaga agctcgacaa actgccgcgc gatcgctggc
     7141 tggagctggg cctgacagac gaagagaaac aaaatcagct ggaacagctg gctgagcagt
     7201 atgacgaact gaaacacgag ttcgagaaga aactcgaagc gaaacgccgc aaaatcaccc
     7261 agggcgacga tctggcaccg ggcgtgctga agattgttaa ggtatatctg gcggttaaac
     7321 gccgtatcca gcctggtgac aagatggcag gtcgtcacgg taacaagggt gtaatttcta
     7381 agatcaaccc gatcgaagat atgccttacg atgaaaacgg tacgccggta gacatcgtac
     7441 tgaacccgct gggcgtaccg tctcgtatga acatcggtca gatcctcgaa acccacctgg
     7501 gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat gctgaaacag cagcaagaag
     7561 tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct gggcgctgac gttcgtcaga
     7621 aagttgacct gagtaccttc agcgatgaag aagttatgcg tctggctgaa aacctgcgca
     7681 aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa agaagcagaa attaaagagc
     7741 tgctgaaact tggcgacctg ccgacttccg gtcagatccg cctgtacgat ggtcgcactg
     7801 gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta catgctgaaa ctgaaccacc
     7861 tggtcgacga caagatgcac gcgcgttcca ccggttctta cagcctggtt actcagcagc
     7921 cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg ggagatggaa gtgtgggcgc
     7981 tggaagcata cggcgcagca tacaccctgc aggaaatgct cactgttaag tctgatgacg
     8041 tgaacggtcg taccaagatg tataaaaaca tcgtggacgg caaccatcag atggagccgg
     8101 gcatgccaga atccttcaac gtattgttga aagagattcg ttcgctgggt atcaacatcg
     8161 aactggaaga cgagtaattc tcgctcaaac aggtcactgc tgtcggggta aaacccggca
     8221 gcggattgtg ctaactccga cgggagcaaa tccgtgaaag atttattaaa gtttctgaaa
     8281 gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg ctctggcttc gccagacatg
     8341 atccgttcat ggtctttcgg tgaagttaaa aagccggaaa ccatcaacta ccgtacgttc
     8401 aaaccagaac gtgacggcct tttctgcgcc cgtatctttg ggccggtaaa agattacgag
     8461 tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg tcatctgtga gaagtgcggc
     8521 gttgaagtga cccagactaa agtacgccgt gagcgtatgg gccacatcga actggcttcc
     8581 ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc gtatcggtct gctgctcgat
     8641 atgccgctgc gcgatatcga acgcgtactg tactttgaat cctatgtggt tatcgaaggc
     8701 ggtatgacca acctggaacg tcagcagatc ctgactgaag agcagtatct ggacgcgctg
     8761 gaagagttcg gtgacgaatt cgacgcgaag atgggtgcgg aagcaatcca ggctctgctg
     8821 aagagcatgg atctggagca agagtgcgaa cagctgcgtg aagagctgaa cgaaaccaac
     8881 tccgaaacca agcgtaaaaa gctgaccaag cgtatcaaac tgctggaagc gttcgttcag
     8941 tctggtaaca aaccagagtg gatgatcctg accgttctgc cggtactgcc gccagatctg
     9001 cgtccgctgg ttccgctgga tggtggtcgt ttcgcgactt ctgacctgaa cgatctgtat
     9061 cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc tggatctggc tgcgccggac
     9121 atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg tagacgccct gctggataac
     9181 ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc ctctgaaatc tttggccgac
     9241 atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc tcggtaagcg tgttgactac
     9301 tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc tgcatcagtg cggtctgccg
     9361 aagaaaatgg cgctggagct gttcaaaccg ttcatctacg gcaagctgga actgcgtggt
     9421 cttgctacca ccattaaagc tgcgaagaaa atggttgagc gcgaagaagc tgtcgtttgg
     9481 gatatcctgg acgaagttat ccgcgaacac ccggtactgc tgaaccgtgc accgactctg
     9541 caccgtctgg gtatccaggc atttgaaccg gtactgatcg aaggtaaagc tatccagctg
     9601 cacccgctgg tttgtgcggc atataacgcc gacttcgatg gtgaccagat ggctgttcac
     9661 gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc tgatgatgtc taccaacaac
     9721 atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt ctcaggacgt tgtactgggt
     9781 ctgtactaca tgacccgtga ctgtgttaac gccaaaggcg aaggcatggt gctgactggc
     9841 ccgaaagaag cagaacgtct gtatcgctct ggtctggctt ctctgcatgc gcgcgttaaa
     9901 gtgcgtatca ccgagtatga aaaagatgct aacggtgaat tagtagcgaa aaccagcctg
     9961 aaagacacga ctgttggccg tgccattctg tggatgattg taccgaaagg tctgccttac
    10021 tccatcgtca accaggcgct gggtaaaaaa gcaatctcca aaatgctgaa cacctgctac
    10081 cgcattctcg gtctgaaacc gaccgttatt tttgcggacc agatcatgta caccggcttc
    10141 gcctatgcag cgcgttctgg tgcatctgtt ggcatcgatg acatggtcat cccggagaag
    10201 aaacacgaaa tcatctccga ggcagaagca gaagttgctg aaattcagga gcagttccag
    10261 tctggtctgg taactgcggg cgaacgctac aacaaagtta tcgatatctg ggctgcggcg
    10321 aacgatcgtg tatccaaagc gatgatggat aacctgcaaa ctgaaaccgt tattaaccgt
    10381 gacggtcagg aagagaagca ggtttccttc aacagcatct acatgatggc cgactccggt
    10441 gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta tgcgtggtct gatggcaaag
    10501 ccggatggct ccatcatcga aacgccaatc accgcgaact tccgtgaagg tctgaacgta
    10561 ctccagtact tcatctccac ccacggtgct cgtaaaggtc tggcggatac cgcactgaaa
    10621 actgcgaact ccggttacct gactcgtcgt ctggttgacg tggcgcagga cctggtggtt
    10681 accgaagacg attgtggtac ccatgaaggt atcatgatga ctccggttat cgaggggggt
    10741 gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg taactgctga agacgttctg
    10801 aagccgggta ctgctgatat tctcgttccg cgcaacacgc tgctgcacga acagtggtgt
    10861 gacctgctgg aagagaactc tgtcgacgcg gtaaaagtac gttctgttgt atcttgtgac
    10921 accgactttg gtgtatgtgc gcactgctac ggtcgtgacc tggcgcgtgg ccacatcatc
    10981 aacaaaggtg aagcaatcgg tgttatcgcg gcacagtcca tcggtgaacc gggtacacag
    11041 ctgaccatgc gtacgttcca catcggtggt gcggcatctc gtgcggctgc tgaatccagc
    11101 atccaggtga aaaacaaagg tagcatcaag ctcagcaacg tgaagtcggt tgtgaactcc
    11161 agcggtaaac tggttatcac ttcccgtaac accgaactga aactgatcga cgaattcggt
    11221 cgtaccaaag aaagctacaa agtgccttac ggtgcggtac tggcgaaagg cgatggcgaa
    11281 caggttgctg gcggcgaaac cgttgcaaac tgggacccgc acaccatgcc ggttatcacc
    11341 gaagtaagcg gttttgtacg ctttactgac atgatcgacg gccagaccat tactcgtcag
    11401 accgacgaac tgaccggtct gtcttcgctg gtggttctgg attccgcaga acgtaccgca
    11461 ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg ctcagggtaa cgacgttctg
    11521 atcccaggta ccgatatgcc tgcgcagtac ttcctgccgg gtaaagcgat tgttcagctg
    11581 gaagatggcg tacagatcag ctctggtgac accttggcgc gtattccgca ggaatccggc
    11641 ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg acctgttcga agctcgtcgt
    11701 ccgaaagagc cggcaatcct ggctgaaatc agcggtatcg tttccttcgg taaagaaacc
    11761 aaaggtaaac gtcgtctggt tatcaccccg gtagacggta gcgatccgta cgaagagatg
    11821 attccgaaat ggcgtcagct caacgtgttc gaaggtgaac gtgtagaacg tggtgacgta
    11881 atttccgacg gtccggaagc gccgcacgac attctgcgtc tgcgtggtgt tcatgctgtg
    11941 actcgttaca tcgttaacga agtacaggac gtataccgtc tgcagggcgt aaagattaac
    12001 gataaacaca tcgaagttat cgttcgtcag atgctgcgta aagctaccat cgttaacgcg
    12061 ggcagctccg acttcctgga aggcgaacag gttgaatact ctcgcgtcaa gatcgcaaac
    12121 cgcgaactgg aagcgaacgg caaagtgggt gcaacttact cccgcgatct gctgggtatc
    12181 accaaagcgt ctctggcaac cgagtccttc atctccgcgg catcgttcca ggagaccact
    12241 cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg aactgcgcgg cctgaaagag
    12301 aacgttatcg tgggtcgtct gatcccggca ggtaccggtt acgcgtacca ccaggatcgt
    12361 atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc aggtgactgc agaagacgca
    12421 tctgccagcc tggcagaact gctgaacgca ggtctgggcg gttctgataa cgagtaatcg
    12481 ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggt gttattcaga
    12541 gggcaaattc ttcacggaaa atattaagaa aggcagggcg aaacgaggaa gaagcttttc
    12601 gccctggaca acattcctgc tgacggcact accataaaaa aacacaacgc tttcaacaac
    12661 cttgcgtttt tcatcttccg tagcggctcg gaataaattt acgttcttac attccgtctc
    12721 atagtctttg cgaggcgcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac
    12781 cctgagcggt taacacggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact
    12841 gttccaactc ggggtgatta tcggcatagc cacctggctg cgttttcgag aaggcgctga
    12901 cgttattgat cgccagcgga ataacgcgat cgcgaaacca cggtgattcc cgcgtggaga
    12961 gtgacagttc aatctccggt gcaagcagtc ggaaggcgca gatggtttgc actaactggc
    13021 gttcatccat aatcgacgca ggctcaatgc cgccagtaca cgggcgcagg cgcggaaagg
    13081 agacagagta acggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca
    13141 tatagcagtc aacgcgccag ttgtcggaaa ggccaattag cgcgccgagg cctatcttat
    13201 caatccccgc acgccccagc cgatccggcg tttccagccg ccagaagaag tcctgttttt
    13261 tgcctttcaa atgatggcgg gtataagtcg cctcgtgata tgtctcctga taaaccatca
    13321 ccccatccag accaagttgc tttaactcgg cgtattccgt ctccgccagc ggttgcactt
    13381 ccatttgtag tgaagagaac tgttcacgca gggcggggag atgacgacga aagtaatcca
    13441 tccccacttt cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc
    13501 gtatagcggc gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcgat
    13561 tactcatgga aaatccgcag tacgtgcagt cgttagcgca aagattggaa agataaagcg
    13621 ggacgtagaa actaactgtg ttgccaaatc gctgacgggt cagacgctgc gcctgttggg
    13681 ccagttgttc cagatagcca ctggcggcag gcgataacag cgccatcatg tcgtcgcggg
    13741 tgagttgcga ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca
    13801 ggcggatgtc gtcccagtcc agttgtcgcc agcgattgct gaaggttttc atgccgatgc
    13861 ctccagaaat ccggtcagcg ggctggtggc atgagcaaaa taactgcggc tgcccggtcc
    13921 ggactgacgt gccagcaggc ctacttctac cgccagacga aatgccttcg ccatgttgac
    13981 gggatcgtcc gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag
    14041 cgcctgcgtg gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaactgtggc
    14101 ctgctggata ataatctcca gcatggcacg ggtttccagt ccctgattcg agccaatcgg
    14161 cgcgccgagc ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg
    14221 atcggccccg cagtaaggca gcacgacaaa tccctgttgt accagcgttt cggcggcttt
    14281 cagggtttcg atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag
    14341 ccagtttgtg cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgctgt
    14401 tttcgccccg gaagtattcg gcagcagggc cacacccgcc gcgataagcg gttcgaggat
    14461 agcgtcgttg tgctggcgca agtcgacacg tttcatcgcc agtgtcacca gctggctgcc
    14521 ggaagcgcgg atcgcctcca ccatcagttg tgaagaagcg aatttccctg tgccggtaaa
    14581 cagatgtgaa tcaaacgttt tgtccgcaat acgtaacatc tcaaccccct gcaataacct
    14641 gaaaaagcag gatctggtcg ccatcctgca cgatatgttg cgtccactgc tcacgcggga
    14701 cgatttgctg attaatcgcc agagccgcgc ccgcttgtcg ttggtcgagt tgctccagta
    14761 gttcgtgaac agtttgcccg gcggcgcact gcatcggttg atcgttaaac aggatctgca
    14821 ttgcttcctc cgcataccgg gcaaccactg gcgcggcgca acgccaggct gcgccactgg
    14881 ctcgacttac cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac
    14941 ttaatggctt ccagtgcctg caaagtgccc atcaccccga ccaccgggcc aaccacgccc
    15001 gccgtgcggc agttgcgttc tggctgctgg ttatctggcc acaggcagcg gtaacacccc
    15061 tgctcccagg gcggtgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg
    15121 ataagcggcg tgttgagtgc cacgcaggcg gcattaatct cctggcgagt cgccatattg
    15181 tcggtacagt cgagcaccac atcggcttgt gcaaccgcat ctttcaacgc ctcacccgtt
    15241 aaccgttgtt gtaatgctgt cagttgaatg tcgggattca actgtgtcag tcgctgttgg
    15301 ctgacctgcg atttcgggct gttgatatct tcagtggtaa agaggatttg tcgttgcaga
    15361 ttgcttaaat gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccctgcc
    15421 aggtatagcg cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg
    15481 agcagttttt gctgcccgtc aagagcgata tcgtcgagca gaatttggcg gctataacgc
    15541 ataaagtcac ggtcattcat cgccaactcc tgcaatttcc agcaactgtg ccgttgccaa
    15601 acgccagtct gcggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc
    15661 tatcaccgca ggcgcgcgtg ccagactgat accgccaatc gccacggtgg gataatccgc
    15721 caggcgctca acatgccgtg ccaactgttc cagcccctgc ggtgcagaag gcatctgttt
    15781 ggtttgcgtc gggaacacat gtcccagcgc gatataagag gggcgtgctg ccagcgcgac
    15841 gtcgatttcc atatcgtcat gtgtcgaaac gcccagccgc aggcctgccg cgcggatggc
    15901 attgagatcg gtggcttgca aatcttcctg ccccaaatgg acgccatacg cctgatgctt
    15961 gatcgcgagc cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc
    16021 aattgccgcc acgacatcgg cttccacctc ttcatcgcgc cgatctttga tgcgtagctg
    16081 gagagtacgt acgcctgcat ccaacagacg ttcgatccac tgtacgctgt ccaccaccgg
    16141 gtacagtcct aaacgaaaag gtacaggagg aaaatcaggc tgatacatca cgcttcctcc
    16201 ttatgcaggt agatttctcc gcctctggca cggaagttct ccgacatatc cgccattccc
    16261 acttcaatgg tttgcgcggc ggcgtaatcg cgtacttcct ggctgatttt catcgagcag
    16321 aattttggcc cacacatgga gcaaaaatgg gcgactttgc cggactcttg gggcagggtt
    16381 tcatcgtgat aagcgcgggc ggtaaacggg tcgagggcca gattaaactg gtcttcccag
    16441 cgaaattcga agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct
    16501 tttgccaggt cagcagcgtg ggcggcaatc ttataagtga taagcccctg cttaacatct
    16561 tctttattgg gcagacccag atgctctttt ggcgttacgt aacagagcat cgcgcagcca
    16621 aaccagccaa tcatcgccgc accaatcccc gacgtgaagt ggtcatagcc cggtgcaata
    16681 tcggtagtta gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg
    16741 gtcatattgc ggcggatcat ctgcatcggc acatggcctg ggccttcaat catcacctgt
    16801 acgtcatatt cccaggcgat tttggtcagt tcgcccagcg tatgcaattc ggcaaactgc
    16861 gcttcatcgt tggcgtcctg aatagaaccg gggcgcagac cgtcgcccag cgacagcgaa
    16921 acgtcataag cggcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc
    16981 tgatgatggg agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc
    17041 aggcgtttcg cggtcatcgg cacatagcgc agcagtacgc ccgcatggat agtgaagtaa
    17101 tccacacctt gctcggcctg ttccagcagc gtgtcgcgga acgcttccca ggtaagatct
    17161 tcggcgatcc cgttaacctt ctccagcgcc tggtagatcg gcactgtacc gatcggcacc
    17221 ggactgttac gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccggt ggagagatcc
    17281 atcaccgtat ccgctcccca gcgcgtggac cataccagct tttccacttc ttcttcgatg
    17341 gaagaggtga ccgccgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca
    17401 ataatcatcg gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca
    17461 gcaacttcat cacggacaaa ttccgcagtg atattttccg gcagatgtgc gccaaagctc
    17521 attcccggat gctggtggcg taaaacctcg ctacggatgc gctcgcggcc catattctcg
    17581 cggatggcga tgaattccat gtccggcgtg atgatgccct ggcgggcgta gtgcagttgg
    17641 gtgacacggc gtcctgtttt ggcgcgtttt ggcgttaata agccgctaaa acgtagctcg
    17701 tcgagaccat catctgccag ccgcgttctg gtgtaatcgg aactgcgcac ggtaagttct
    17761 tcggtatcgg cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg
    17821 ttaatggcga tctgcggatc accatacggg ccggaggtgt cgtagaccgg aatcgcttcg
    17881 ttttcttcgt actgcggctg ttctttgcta ccgccaatta gcgtcgggct aagctggatc
    17941 tcacgcatcg gcacgcgcac gccggggtgt gtgccagtga tataaatgcg ttttgagttg
    18001 ggaaaggcgg tgccttccag ggtgtcgata aaatgttggg cccgggcgcg ttgttcgcgg
    18061 cgggtcagtt ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga
    18121 cgacggatga agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt
    18181 tcccttcgca ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca
    18241 gttaaggcac tccgacaaga aattagcccc cacaaacggg gcgttgaatg taaattacgc
    18301 gttaacagcg cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa
    18361 tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct
    18421 gttgaaactc aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtaat
    18481 ccataatctg ttgggtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt
    18541 gcccgttgcc ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca
    18601 agtaatcgac caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg
    18661 attctttgcc aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta
    18721 catgtagcca gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga
    18781 gctggttaag catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt
    18841 aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa
    18901 ttgaaaaatt agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga
    18961 agggagaatt gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac
    19021 aaatcggcga atggcagggg gaacctgttt ggttaataca acagcagcgg cgtcacgata
    19081 tggggtcggt acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg
    19141 gcgtgcaact ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa
    19201 tgtatccgag caaaaccgaa tgggcgatgc tgtgtagcca ttgccgtgag cgttactacc
    19261 cgcaaatcgc cccctgcatt attgttgcca tccgtcgcga tgattcgctc ctcctcgccc
    19321 agcatacccg ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggcg
    19381 aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa
    19441 acttgcgtta cgtgacttct cagccgtggc cgtttcctca gtctttaatg accgcgttta
    19501 tggcggaata tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact
    19561 ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga
    19621 tagaagatac tgtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga
    19681 cgcactaagg aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc
    19741 tgcgccagcc cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac
    19801 cggaatataa agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg
    19861 agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc
    19921 tcttttccga tatcctcacc gtgccggacg cgatggggtt agggctctat tttgaagccg
    19981 gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa
    20041 ttccggaccc ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcacg
    20101 aactgaaagg cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcaacct
    20161 acatggtgga aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg
    20221 atccgcaggc gctgcacgct ctgctcgata aactggcgaa aagcgtcact ttgtatctga
    20281 atgcgcagat taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc
    20341 ttaccgggcg cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt
    20401 tactgcgtga aaacgacggt cgccgcgtgc cagtcacgct gtttaccaaa ggcggcggac
    20461 agtggctgga agccatggct gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg
    20521 acatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc
    20581 cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag
    20641 gtttcggtca cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc
    20701 cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc
    20761 gctaagagcc tgatccatca ggctatttta tttgccattt tgaggccggg cagtgctcaa
    20821 aatcctcacg tactacgtgt acgctccggt ttttccgcgc tgtccggcct caaactgcct
    20881 tcaacaatta cgcctgttga accaggctct aaggagcgat tatggatctc gcgtcattac
    20941 gcgctcaaca aatcgaactg gcttcttctg tgatctgcga ggatcgactc gataaagatc
    21001 caccggatct gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag
    21061 cggcgatggt gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gttgcccgca
    21121 tcgccaccac catgccttac attccaggtt ttctttcctt ccgcgaatat cctgcgctgc
    21181 tggcagcgtg ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga
    21241 tctcgcatcc tcgccgtctt ggcgtcgcca gccattttgg cttattggtg gatgtgccga
    21301 ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcaaaccgg
    21361 gcgcgctggc cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag
    21421 cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg
    21481 cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg
    21541 cggtggcctc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt
    21601 aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc
    21661 gtctggagcg cctggaaagc tggcagcatg tcactttcat ggcttgctta tgcgaacgca
    21721 tgtaccccaa ttacgccatg ttctgccagc aaaccggttt tggtgatggg caaatttacc
    21781 gtcgtattct cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg
    21841 acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg
    21901 gcgtttatcc ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga
    21961 gtggtgaaac gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga
    22021 tgctggaaat gactcaggct ggtcgcgaaa tgggcgatga agagcttaaa gaaaacccag
    22081 ctgtagagca agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag
    22141 aacgcgatat cgagctgata aaaggcctta gggcagacct gcgtgagtcg ggtgagagca
    22201 atattggtat aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg
    22261 tacctggagt cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg
    22321 gctatcggtg cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt
    22381 agcaagcgat aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa
    22441 ttgcagagaa agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg
    22501 ctgcaattac tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct
    22561 tcaaagtgaa ccatcgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca
    22621 aaattgccgc agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta
    22681 agtaagattg cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt
    22741 tctggcgtcg atcattgatg ctggcaggtg cgctgtttct cactgcctgt agccacaact
    22801 cttcacttcc tccctttacc gccagtggat ttgctgaaga ccagggcgcg gtacgcatct
    22861 ggcgaaaaga cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg
    22921 gcgataccac gacgcgagag tatcgctggc agggcgataa cctcacgctc atcaatatca
    22981 atgtttacag caaaccgccg gtgaacattc gcgcgcgttt tgacgatcgc ggcgatctga
    23041 gctttatgca acgtgaatcc gatggggaaa agcagcagct ttctaacgac caaatcgatt
    23101 tataccgtta tcgtgctgat cagatccgcc agattagcga tgccttacgt caggggagag
    23161 tcgtgctgcg ccaggggcgc tggcatgcga tggaacagac cgtgaccacc tgcgaagggc
    23221 aaaccattaa acctgattta gattcgcagg cgatagcgca tatcgagcgc cgccagagcc
    23281 gctcttctgt tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg caattactgt
    23341 tagtggcaaa ctctgatttc tgtcgctggc aacccaacga gaaaacgttc tgatttacca
    23401 gtggcccata cccatatgac cgccaccacc gcagccgccg tagcccatgc ccattccggc
    23461 tccgcgcgga atacccgctt cagccatcgc gatatctcgt ttcacccgta actcatctaa
    23521 cgactgacgc aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg
    23581 gttcgcggct aacagggcat tgtattcata acgcttcgtc accagttgct gttgcagtgc
    23641 gctgctttga gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt
    23701 caaaggcgcg gcattttgct gccacatacc gtgtccgccg tgagcaaatg cagatgtcga
    23761 tcccatcgcc attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat
    23821 ggttaatcct ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa
    23881 cgctgatatg acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg
    23941 tagcgagtca tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc
    24001 tgataaaaga tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg
    24061 cgttttatgc aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg
    24121 gtcattgttg ggctggtagg gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag
    24181 actgccgccg ccagacaaac gctgctggaa aaaggcagtg tacttattcg cgctcttgaa
    24241 tccggctcgc gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggccttactg
    24301 gaagaaatgg ccgggcagcc tggagtacgt tggtttgcgg tcacggatga acaaggaaca
    24361 atcgtgatgc atagcaactc cggcatggtg ggaaaacagc tttattcccc tcaggaaatg
    24421 cagcagttac atccgggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag
    24481 cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gtaccggatg
    24541 cgccatatgc agcagtatgc cgcgacacca caagcaattt tcatcgcttt cgacgccagt
    24601 aatattgtga gtgccgaaga tcgtgagcag agaaacaccc tgattattct cttcgccctg
    24661 gcgacggtct tgctggcaag cgtattgtca ttcttctggt atcgccgcta tctgcgctcg
    24721 cgccagcttc tacaagatga aatgaagcgc aaagagaagc tggtggcgct ggggcatctt
    24781 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg actggcgaaa
    24841 tactttgccg agcgcgcgcc tgcaggggga gaagcgcatc aactggcgca ggtgatggcg
    24901 aaagaggccg accgtttaaa ccgcgtggta agcgagttgc tggaactggt taagccaacg
    24961 catctggctt tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta
    25021 agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg
    25081 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    25141 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg ccagcgaaag cggcgcgggc
    25201 gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg cagatcagct tgaagccatc
    25261 ttcactccgt acttcaccac caaagccgaa ggcaccggat tggggctggc ggtcgtgcat
    25321 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggaggg aaaaggctca
    25381 acgttcaccc tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg
    25441 ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt
    25501 tactgcgcgg ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc
    25561 aggtgcggga acaggttttt gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg
    25621 gcatcgccac gctgaaagag atcaaagcgt taaacccggc aattccggtg ctgattatga
    25681 ctgcgtactc cagcgtcgag acggcggtag aggcactgaa aactggggcg caggattatc
    25741 tcatcaagcc gctggatttc gataacctac aggcgacgct ggaaaaggcg ctcgcgcata
    25801 cgcacagtat tgatgctgaa acacctgcgg tgactgccag ccagttcggt atggtcggta
    25861 aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    25921 cggtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg
    25981 ccagtagcgc acgtagcgaa aaaccgctgg taatgctcaa ctgtgcggca ctcaacgaat
    26041 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttactgga gccgataaac
    26101 gccgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    26161 atatctcgcc gatgatgcag gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    26221 gtgtcggtag caaccagact atctcggttg atgtccggct gattgcggcg acccatcgcg
    26281 atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaatg
    26341 tggtggcgat tgaagtacca tcgctgcggc aacggcggga agatatccct ctgctggctg
    26401 gccattttct tcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggc tttacgccac
    26461 aggcgatgga tttgctgatc cactacgact ggccgggaaa tattcgtgaa ctggaaaacg
    26521 cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc
    26581 tggcgattgc cagtacgccg atcccgctgg ggcaaagtca ggatattcag ccgctggtgg
    26641 aagtggaaaa agaggtgatt ctggcggcgc tggagaaaac gggcggcaac aaaaccgaag
    26701 ccgcccgtca gttagggatc acgcgcaaaa cgctattggc aaaactgtcg cgttagttct
    26761 gctcgcgttc gatggcgcgc caaccgatat ctttccggca gaagcaatca tcccagtgga
    26821 tatcggtcat taaggcgtag gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    26881 tgacgcacag tacgcgcccg ccgctggtta ccacctgctc gtcatccgcc agttttgtgc
    26941 ccgcgtggaa cactttgccg tcagccactt cttccagcgg caggccgtgg atcacatcgc
    27001 cggtgcggta atcgcccgga tatccacccg cagccatcac cacgctgaga gaagcacgtt
    27061 catcccactc tgacgttttc tcgtccagtt tgccttcaca ggccgccagg cagagctcaa
    27121 ccagatcgga cttcatgcgc agcataatcg gctgggtttc cggatcgcca aagcggcagt
    27181 taaattcaat aaccttcgga ttgccctgtt tgtcgatcat caggcccgca tagagaaaac
    27241 cggtgtaggt gttgccttcc gccgccatgc ctttcacggt tggccagatg atacgttcca
    27301 tggtgcgctg atgaacttca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc
    27361 cggtgtttgg tccgttatct ttatcgccta cgcgtttgtg atcctggctg gtagccatcg
    27421 gcagcacatg ctcgccgtcc accatcacga taaagctcgc ttcttcgcca tcgaggaact
    27481 cttcgataac gatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa
    27541 cagccgcttc cgcttcttcc agcgtcatcg ccacgataac gcctttcccg gcagccagac
    27601 cgtccgcttt aatgacgatt ggcgcgcctt tctcacgcag atacaccagc gcaggttcta
    27661 cctcggtgaa gttctggtat tccgccgtag ggatcttatg gcgggccagg aaatctttgg
    27721 taaacgcttt tgagccttcc agttgggccg cacctgcggt tgggccgaag attttcagcc
    27781 cggcggcgcg gaaggtatcg accacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    27841 tcagatcaat cttttcgttt tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccga
    27901 tagcaacgtt ttgcagcgcg ggttccagcg cagtgcctgc attacccgga gcaacaaaaa
    27961 cagtctcaac cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt
    28021 taccaatcac taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt
    28081 gaagagcatc gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggatgga
    28141 accgccaggc tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg
    28201 gaacgggaag aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcggc
    28261 agctttaata ccggcgattt tcgcggagta cacgcggctc atctggcccg cgccaatacc
    28321 gatggtcata ttgtttttgg catagacgat agcattggat ttcacgaatt tcgccacttt
    28381 ccagcagaac agcgcatcac gcagttcctg ttcggtcggc tgacgttggg tgacgacgcg
    28441 cagttcttcc gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac
    28501 gcgtttgaaa tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac
    28561 gttctgcttg gcggcggtga ttttcagggc ttcttcgctg gcggaaggcg caataatcac
    28621 ttcgacaaac tgacgagaaa tgatggcctg tgcggtttcc gcatccagct cgcggttaaa
    28681 ggcaatgatg ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc
    28741 aagaatggaa ttgccgatag ctacaccgca agggttggcg tgcttcacaa tcacacatgc
    28801 cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    28861 agagagggct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc
    28921 ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt
    28981 aatgaagttc aggttcagcg tgcgtgggaa gcgaccggcg gcttctttgc tttcaccgtg
    29041 gtacgccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg
    29101 ttcgaaggct ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc
    29161 atccatctct ttaataatgg cgtcgtagtc gctgctcttc accacgattg cgacatcttt
    29221 atggttctta gcggcggagc gcaccatggt cgggccgccg atatcgatgt tctcaaccgc
    29281 atcttccagc gagcagcctt cacgagcaac agtctgggcg aacggataca ggttaacaac
    29341 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    29401 gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    29461 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    29521 ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    29581 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    29641 catggtaaat cccctgggtt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    29701 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    29761 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggttac
    29821 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    29881 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg
    29941 cggcctgcgg agaactccct ataatgcgcc ttcatcgaca cggcggatgt gaatcacttc
    30001 acacaaacag ccggttcggt tgaagaga
//
LOCUS       DADLDT020000044        29797 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000044 DADLDT020000000
VERSION     DADLDT020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.044"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            56..850
                     /locus_tag="JQH22_001855"
     CDS             56..850
                     /locus_tag="JQH22_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFB2206191.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="JQH22_001856"
     CDS             872..2281
                     /locus_tag="JQH22_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206192.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="JQH22_001857"
     CDS             2259..3038
                     /locus_tag="JQH22_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFB2206193.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="JQH22_001858"
     CDS             3035..3895
                     /locus_tag="JQH22_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFB2206194.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="JQH22_001859"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="JQH22_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="JQH22_001860"
     CDS             complement(4635..4895)
                     /locus_tag="JQH22_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFB2206195.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="JQH22_001861"
     CDS             complement(4886..6157)
                     /locus_tag="JQH22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFB2206196.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="JQH22_001862"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="JQH22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFB2206197.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="JQH22_001863"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="JQH22_001863"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFB2206198.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="JQH22_001864"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="JQH22_001864"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFB2206199.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="JQH22_001865"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="JQH22_001865"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFB2206200.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="JQH22_001866"
     CDS             11499..11651
                     /locus_tag="JQH22_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFB2206201.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="JQH22_001867"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="JQH22_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JQH22_001868"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JQH22_001868"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFB2206202.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="JQH22_001869"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="JQH22_001869"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFB2206203.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="JQH22_001870"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="JQH22_001870"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFB2206204.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="JQH22_001871"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="JQH22_001871"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFB2206205.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="JQH22_001872"
     CDS             17363..17686
                     /locus_tag="JQH22_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFB2206206.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="JQH22_001873"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="JQH22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFB2206207.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="JQH22_001874"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="JQH22_001874"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFB2206208.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="JQH22_001875"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="JQH22_001875"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFB2206209.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="JQH22_001876"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="JQH22_001876"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFB2206210.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="JQH22_001877"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="JQH22_001877"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFB2206211.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="JQH22_001878"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="JQH22_001878"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFB2206212.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="JQH22_001879"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="JQH22_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFB2206213.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="JQH22_001880"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="JQH22_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFB2206214.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="JQH22_001881"
     CDS             complement(24261..24668)
                     /locus_tag="JQH22_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFB2206215.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="JQH22_001882"
     CDS             24815..25408
                     /locus_tag="JQH22_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFB2206216.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="JQH22_001883"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="JQH22_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="JQH22_001884"
     CDS             complement(26628..27191)
                     /locus_tag="JQH22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206217.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="JQH22_001885"
     CDS             27356..27607
                     /locus_tag="JQH22_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2206218.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="JQH22_001886"
     CDS             27609..29705
                     /locus_tag="JQH22_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2206219.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7300 a         7381 c         8058 g         7058 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cttgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaacccttta cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DADLDT020000045        29634 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000045 DADLDT020000000
VERSION     DADLDT020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29634
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.045"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..186)
                     /locus_tag="JQH22_001887"
     CDS             complement(<1..186)
                     /locus_tag="JQH22_001887"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFB2206220.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQH22_001888"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQH22_001888"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFB2206221.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="JQH22_001889"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="JQH22_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFB2206222.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="JQH22_001890"
     CDS             3863..4144
                     /locus_tag="JQH22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFB2206223.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4437..4961
                     /locus_tag="JQH22_001891"
     CDS             4437..4961
                     /locus_tag="JQH22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFB2206224.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5042..5716
                     /locus_tag="JQH22_001892"
     CDS             5042..5716
                     /locus_tag="JQH22_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFB2206225.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5732..8212
                     /locus_tag="JQH22_001893"
                     /pseudo
     CDS             5732..8212
                     /locus_tag="JQH22_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8228..9261
                     /locus_tag="JQH22_001894"
                     /pseudo
     CDS             8228..9261
                     /locus_tag="JQH22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="JQH22_001895"
     CDS             complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="JQH22_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFB2206226.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="JQH22_001896"
                     /pseudo
     CDS             complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="JQH22_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10870..11142
                     /gene="rcnR"
                     /locus_tag="JQH22_001897"
     CDS             10870..11142
                     /gene="rcnR"
                     /locus_tag="JQH22_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFB2206227.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11234..11330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11365..12153
                     /gene="thiM"
                     /locus_tag="JQH22_001898"
     CDS             11365..12153
                     /gene="thiM"
                     /locus_tag="JQH22_001898"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFB2206228.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12150..12950
                     /gene="thiD"
                     /locus_tag="JQH22_001899"
     CDS             12150..12950
                     /gene="thiD"
                     /locus_tag="JQH22_001899"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFB2206229.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13015..13836
                     /locus_tag="JQH22_001900"
     CDS             13015..13836
                     /locus_tag="JQH22_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFB2206230.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13884..14630
                     /locus_tag="JQH22_001901"
     CDS             13884..14630
                     /locus_tag="JQH22_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFB2206231.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGGLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14604..15569)
                     /locus_tag="JQH22_001902"
     CDS             complement(14604..15569)
                     /locus_tag="JQH22_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFB2206232.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15566..16569)
                     /locus_tag="JQH22_001903"
                     /pseudo
     CDS             complement(15566..16569)
                     /locus_tag="JQH22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16566..17843)
                     /locus_tag="JQH22_001904"
     CDS             complement(16566..17843)
                     /locus_tag="JQH22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206233.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18100..19152
                     /gene="fbaB"
                     /locus_tag="JQH22_001905"
     CDS             18100..19152
                     /gene="fbaB"
                     /locus_tag="JQH22_001905"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFB2206234.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19460..20314
                     /gene="gatY"
                     /locus_tag="JQH22_001906"
     CDS             19460..20314
                     /gene="gatY"
                     /locus_tag="JQH22_001906"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFB2206235.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20343..21605
                     /gene="gatZ"
                     /locus_tag="JQH22_001907"
     CDS             20343..21605
                     /gene="gatZ"
                     /locus_tag="JQH22_001907"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFB2206236.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21615..22067
                     /gene="gatA"
                     /locus_tag="JQH22_001908"
     CDS             21615..22067
                     /gene="gatA"
                     /locus_tag="JQH22_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFB2206237.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22098..22382
                     /gene="gatB"
                     /locus_tag="JQH22_001909"
     CDS             22098..22382
                     /gene="gatB"
                     /locus_tag="JQH22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFB2206238.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22386..23741
                     /locus_tag="JQH22_001910"
     CDS             22386..23741
                     /locus_tag="JQH22_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFB2206239.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23789..24829
                     /gene="gatD"
                     /locus_tag="JQH22_001911"
     CDS             23789..24829
                     /gene="gatD"
                     /locus_tag="JQH22_001911"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFB2206240.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24935..25708
                     /locus_tag="JQH22_001912"
     CDS             24935..25708
                     /locus_tag="JQH22_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HFB2206241.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="JQH22_001913"
     CDS             complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="JQH22_001913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFB2206242.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27095..27412
                     /locus_tag="JQH22_001914"
     CDS             27095..27412
                     /locus_tag="JQH22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206243.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="JQH22_001915"
     CDS             complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="JQH22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFB2206244.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29262..29593)
                     /locus_tag="JQH22_001916"
                     /pseudo
     CDS             complement(29262..29593)
                     /locus_tag="JQH22_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7699 a         6878 c         7480 g         7577 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4201 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4261 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4321 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4381 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4441 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4501 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4561 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4621 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4681 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     4741 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     4801 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     4861 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     4921 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     4981 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5041 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5101 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5161 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5221 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5281 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5341 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5401 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5461 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5521 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5581 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5641 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     5701 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     5761 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     5821 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     5881 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     5941 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6001 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6061 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6121 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6181 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6241 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6301 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6361 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6421 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6481 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6541 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6601 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6661 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     6721 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     6781 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     6841 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     6901 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     6961 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7021 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7081 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7141 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7201 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7261 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7321 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7381 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7441 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7501 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7561 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7621 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7681 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     7741 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     7801 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     7861 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     7921 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     7981 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8041 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8101 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8161 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8221 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8281 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8341 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8401 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8461 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8521 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8581 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8641 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     8701 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     8761 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     8821 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     8881 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     8941 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9001 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9061 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9121 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9181 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9241 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9301 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9361 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9421 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9481 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9541 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9601 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9661 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
     9721 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
     9781 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
     9841 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
     9901 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
     9961 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10021 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10081 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10141 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10201 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10261 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10321 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10381 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10441 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10501 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10561 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10621 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10681 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    10741 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    10801 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    10861 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    10921 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    10981 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11041 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11101 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11161 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11221 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11281 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11341 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11401 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11461 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11521 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11581 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11641 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    11701 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    11761 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    11821 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    11881 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    11941 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12001 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12061 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12121 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12181 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12241 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12301 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12361 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12421 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12481 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12541 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12601 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12661 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    12721 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    12781 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    12841 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    12901 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    12961 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13021 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13081 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13141 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13201 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13261 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13321 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13381 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13441 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13501 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13561 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13621 accaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ttatcaacgt
    13681 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
    13741 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggggat gagttgcagg
    13801 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
    13861 actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
    13921 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
    13981 aaaatgccgt gcgcagcggg gggctggagc atggcaatat tttgcccggt gagcgtgatt
    14041 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
    14101 aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
    14161 actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgtgcggg aaaaagcccg
    14221 atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg
    14281 cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
    14341 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
    14401 gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
    14461 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
    14521 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    14581 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    14641 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    14701 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    14761 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    14821 gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca
    14881 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    14941 gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc catgatccgc
    15001 gccagtaatg cgtccgggat atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg
    15061 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    15121 ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca ttggcgattc
    15181 cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt
    15241 gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa cccctctttc
    15301 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    15361 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    15421 acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    15481 atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc
    15541 agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    15601 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc gcctttaacg
    15661 caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta
    15721 tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt gggtcggttt
    15781 gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg gtgcctgcgc
    15841 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta cgcacaattt
    15901 tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct
    15961 gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa
    16021 tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat
    16081 gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca cgcgctggaa
    16141 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca
    16201 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    16261 gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta cgcccaatca
    16321 gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac aggccatcga
    16381 ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc
    16441 aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc
    16501 ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc
    16561 tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac cttgatagcc
    16621 gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc
    16681 atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt taccggttcc
    16741 tgataagcga acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg
    16801 aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg cacgggggct
    16861 tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc
    16921 aaaataccga ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag
    16981 aagccatagc agatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag
    17041 cgtttagtga aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc
    17101 atccagccgg tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa
    17161 tagaacgcca gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta
    17221 tcgcgcagca ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg
    17281 ctttttggcg gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agaacttccg
    17341 gcggtaatca gcagcgggat gttagtcggt gagatatcgg cataccccag catttgcggc
    17401 aagaaaccac atgccagacc ggaggcaatc cagccgatag tgcccatcac acgaatgcgt
    17461 gggaaatcac gctccacatc cggcacgttg gcaaaagcga tgctgttagt cagcgcaatg
    17521 gtcggcatat aggttagcga gtaggccagc agtaacggaa agaacccggc aaaagtggtc
    17581 tgttgcgcag cgaaatacat cagcacagca ccagcgaaca tcaataccgc cagcactttc
    17641 tgcgccgaga aaaagcggtc agtgatggag ccaaccagaa ttggcgacag gatcgccgca
    17701 atggctgtgc aggcatacga ccagccaatt tctccggcac taaaaccgct tttacttaac
    17761 cacaaccaca atggcacaaa ccacgcgccc cagataaacc attcaacaaa catcatgaac
    17821 gacagctttg ctgttgtttt catcttttaa ttccttcatg acaggtaatg taaggtacgc
    17881 gatggcaata ccacataata atgccttttg aatacctttg cggtgaaaac ttgaccgcgc
    17941 taacattttt tctgatgaat cgagccaaca gaaaacgctg aaaaaacatc caaaagatgg
    18001 aaaaactcgc ccgtaacgac taggattatg attgcccgca aaaggcgggc caggacaatc
    18061 catagccgat atccaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    18121 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    18181 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    18241 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    18301 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    18361 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    18421 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    18481 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    18541 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    18601 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    18661 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    18721 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    18781 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    18841 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    18901 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    18961 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    19021 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    19081 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    19141 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    19201 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    19261 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    19321 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    19381 ttgtgatcat tatctcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    19441 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    19501 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    19561 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    19621 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    19681 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    19741 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    19801 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    19861 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    19921 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    19981 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    20041 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    20101 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    20161 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    20221 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    20281 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    20341 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    20401 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    20461 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    20521 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    20581 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    20641 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    20701 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    20761 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    20821 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    20881 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    20941 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    21001 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    21061 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    21121 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    21181 tcggtcccgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    21241 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    21301 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    21361 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    21421 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    21481 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    21541 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    21601 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    21661 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    21721 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    21781 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    21841 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    21901 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    21961 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    22021 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    22081 aataaaaagt actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    22141 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    22201 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    22261 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    22321 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacaggggt
    22381 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    22441 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    22501 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    22561 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    22621 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    22681 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    22741 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    22801 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    22861 agctctgcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    22921 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    22981 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    23041 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    23101 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    23161 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    23221 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    23281 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    23341 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    23401 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    23461 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    23521 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    23581 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    23641 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    23701 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    23761 ttatccccct taacaatcag gagtttttat gaaatcagta gtgaatgata ctgatggtat
    23821 cgtgcgcgtt gcaaaaagcg tcattcctga aattaaacat caggatgagg tgcgggtaaa
    23881 aattgccagc tcgggcttat gtggttccga tttacccagg atatttaaaa atggtgcaca
    23941 ttattatcca ataacgttag gccatgaatt tagcggctat attgatgcgg tgggatccgg
    24001 tgttgatgat ttacaccctg gcgatgcggt tgcctgtgtg ccgttattac cctgttttac
    24061 ttgtccagag tgtttgaaag ggttttattc ccagtgcgca aaatatgatt ttattggctc
    24121 gcggcgtgat ggtggatttg ctgaatatat tgtcgttaag cgaaaaaatg tctttgctct
    24181 acccacggat atgcctattg aggatggggc ttttattgag ccgattaccg ttggcctgca
    24241 tgcttttcat ttagcgcaag gttgtgagaa taaaaacgtt attattattg gtgccggaac
    24301 cattggcctg ctggctattc agtgcgctgt cgcgctggga gcaaagagtg tgacggcgat
    24361 cgacattagc tcagaaaaac tggcactggc aaaatctttc ggtgcgatgc aaacatttaa
    24421 cagtagcgaa atgagcgcgc cgcaaatgca gagcgtttta cgcgaactgc gctttaatca
    24481 gcttatcctc gagacggctg gcgtaccgca aactgtcgaa ctggcggtag agattgccgg
    24541 tcctcatgcc caactggcgc tggtgggcac gttgcatcag gatctgcatt taacatcggc
    24601 aacgtttggc aaaatattgc gtaaagagct gacggttatc ggcagttgga tgaactattc
    24661 cagcccttgg ccggggcagg agtgggaaac ggcgagccgg ttgctgacag aacgtaagtt
    24721 aagcctggag ccattaatcg ctcaccgtgg aagctttgaa agcttcaccc aggcggtgcg
    24781 tgacatcgct cgtaatgcca tgccgggcaa agtgttgctc attccatgaa accgcgggcc
    24841 agcgtgatgc tggcccggta ttgtgcaaaa cagatcattc accaatggtc ccccttcgtt
    24901 tacactagcc acaattgaat atgggtaaat gacgatgaat tcattcgagc gaaggaataa
    24961 gatcatccaa ttagtgaatg aacagggaac cgtgcttgtt caggatctgg cgggagtatt
    25021 tgctgcctcg gaagcgacaa tccgtgccga tttgcgcttt ctcgaacaaa aaggcgtggt
    25081 tacgcgcttt catggcggtg cggcgaaaat aatgtccggt aatagtgaaa ccgagaccca
    25141 ggaagtcggg tttaaagagc gatttcagct cgccagcgcg ccaaaaaaca gaatagcgca
    25201 ggcggcagtc aaaatgatcc atgaagggat gaccgttatt ctcgacagcg gaagtaccac
    25261 aatgcttatc gccgaaggat taatgaccgc taaaaatatc acggtgatta ccaacagtct
    25321 cccggcagcg tttgcccttt ccgaaaataa agacattact ctggtcgtct gtggtggcac
    25381 ggtccgccat aaaacgcgct cgatgcatgg ttccattgcc gagcgttcat tgcaggatat
    25441 taatgccgat ttaatgtttg tcggtgctga tggtattgat gcggttaatg gtattacgac
    25501 ctttaatgaa ggttattcga ttagcggggc gatggtcacc gccgcaaata aagtgattgc
    25561 tgttctcgat tcatcgaaat ttaatcgtcg cggttttaat caggtactgc caatcgaaaa
    25621 aattgacatt attgttaccg atgatgctgt gtcagaggtg gataaattgg cattgcagaa
    25681 gacacgggta aaattaatta cggtgtagta ttgttctgta tgccggataa ggtgtttaca
    25741 ccgcatccgg cattacggat tctccctggt tcaagagaga atagtttgat taacgcaata
    25801 atggacaatc tggtggtaat cgacaacgca acgccgccgg aagtatttca atatggaagt
    25861 tttgcccact caacggttcg ccatcaagat taaaagtgat ttcgtgtggg gcttgtatat
    25921 caaaccacga cgaagcgcct tcgataatat tcgggttatc ttcgtcagat tttaaggttg
    25981 atacgagagc cggaagaatt tcatcgccgg taaaaatgcg cagttgcagc aagccatcgt
    26041 taattaacgc gttcgggcac aattgttgac cgccaccggc ctgacgcccg ttaccaatac
    26101 caatgaccag ggcgtcacct tgccagtgaa agttttcacc gcggatttca caacggtccg
    26161 gttgcagagt atccatgcgc attaagccat gaatgatgta agagacgcca cccagcgcgg
    26221 cttttaattt ttccggcgtt tctgtggtaa tacgcgtccc aaatccgcct gtcgccatat
    26281 taataaaaca ggtttgtttg ttgacctgcg ccatatctat cgcaatggca ttaccggcaa
    26341 ttgccagttt cagcgcctta tccagtgcct caggaatccc tacactggtg gcaaaatcat
    26401 tggcggttcc taatggcaaa attcccagcg cgggtatgtc atccccctca cactgaatca
    26461 acgccgtaga aacttcatta atggtgccat cgccaccacc ggcaatcacc gttgcgacgc
    26521 ccaacttccg ggcctcctct acaaatcgtg cggcatcacc tttctcccag gtgacccgca
    26581 catggatcgt cattccttcc tcacgcaaca gcataattgc ttcgcgcaag ggtagattgt
    26641 cagtactttt accattaaga atcagtaagc tggcgggaaa ttctgccatg atcgtatgtg
    26701 cctttatgat tggtctgtag atagtgtaga gcagaaaaca aaaggtggag tcagaacagg
    26761 atgaaagtcg gaggataaaa agtcagttca agtatggagg attcagtggt taccaccagg
    26821 agcttgcaat ggggacagcg aatactaacg gctgccaccg ataaatttca aaaaagagca
    26881 tatacctaat attcaactaa acagtggcat cttcaatata atatattaaa gcccccatgg
    26941 agttaccctg aagggcctca atgtccgtaa ttcctactta tgtaggaaat gttgtacaga
    27001 gcatttatta taatcctatt caattataat aatcatgcca ttattatatt taaacactag
    27061 agagtgtcgt tggtatttaa tgggggaagg tgagatgaaa aagatagctg ctatatcatt
    27121 aattagtatt tttcttatat ctggttgtgc tgtgcataat gatgaaacaa gtatcggtaa
    27181 atttggtctt gcatataaaa gtaatattca gcgtaaactc gataaccaat actacaccga
    27241 agccgaagct tctttagcca ggggcagaat atctggtgca gaaaatatag taaaaaatga
    27301 tgcagcccat ttctgtgtta ctcagggcaa aaaaatgcag atagttgacc tgaagacaga
    27361 aggtgcagga ttacatggcg tcgctcgtct gacattcaaa tgtggagagt gagaatattt
    27421 ttttgtaagc gtcaaacatg cgcgttctgg ctgtgcgtag ccggaacctg ggcgagcacg
    27481 ataccgttac gtgaaaggca ttgtgctatg aagggggatt ctatcgatgt ggtcaatgga
    27541 agacggttat cagggatcaa tccttcttta cagcttatgt acaaaaaata agcccgtgta
    27601 agggagatta cacaggctaa ggaggtggtt cctggtacag ctagcatttt atggattatg
    27661 tttttcagcg aaacggatga taaccttaat aaatgcagct gtatgtgatc ggtttctaag
    27721 aattttccat ccgggaaaaa taatcgaaat taatcactta ccgtgggggt tacgcgtggt
    27781 ttccccggag aaattacgca ttagcagcgc gtaatttagc tcaagatcct gcgggaccgg
    27841 gagccacaca gtataaccat cgcctggtgc gaccggcata gcttcgcctt tggcgttttc
    27901 catgtgctca agggtaaagt taatgttgcc ttgcggcgtc atcagctcaa gactgtcgcc
    27961 aacggagaat ttatttttca ccgctaccgc tgcgaggtcc cccttgcgct caccggtaaa
    28021 ctcaccaaca aactgctggc ggtcagaaac tgaataaccg tattcgtagt tctgataatc
    28081 gtcgtgagta tgacgacgca ggaaaccttc ggtatagcca cgatgcgcca gaccttccag
    28141 agtttccagc aggctggtat cgaacggttt tcccgcagcg gcgtcatcga tagctttgcg
    28201 gtaaacctgt gcggtgcgtg cgcaatagta gaaagatttg gtacgacctt cgattttcag
    28261 cgaatgcacg cccattttgg tcaggcgttc tacatgggcg atggcgcgca gatctttcga
    28321 gttcatgatg taagtgccgt gctcatcttc aaacgcggtc atatactcgc ccggacgctg
    28381 ggcttcttcg atcataaaca ctttgtcggt tggcgcgccg atacccagcg tcggctcaac
    28441 attttgcacc ggaatcggct cgtacttgtg tacgatgttg ccaacgtcat cttctttccc
    28501 ttcctggacg ttgtactccc agcggcaggc gttggtgcag gtgccctggt tcgggtcgcg
    28561 cttgttgata tagccagaga gcaggcagcg accggagtag gccatgcaca gtgcgccgtg
    28621 aacgaagatc tcgatctcca tatccggcac ctgattatgg atctcttcaa tctcttccag
    28681 cgacagctcg cgagagagga tcacgcgggt cagtcccatt tgctgccaga atttcaccgt
    28741 cgcccagttc acggcgttag cctgcaccga gaggtggatc ggcatttcag ggaagtgctc
    28801 acgcaccagc ataatcagcc ctggatcgga cataatcagc gcatccggcc ccatttccac
    28861 caccggtttc aggtcacgga taaaggtttt cagcttggcg ttgtgcggtg caatgttgac
    28921 cacgacataa aactttttcc ccagcgcgtg ggcttcattg atgccgagct gaagattttc
    28981 gtggttgaat tcgttgttgc gcacacgtag ggagtaacgc ggctggcccg cataaacagc
    29041 atctgcgcca taagcgaaag cgtaacgcat atttttcagc gttcccgccg gggaaaggag
    29101 ttccggttta aacataattc tctcgttctg atgacaggtc agatccgctt cacctgatga
    29161 agcggttagg ggaggccccc actttaaggg cgggcattgt agcgctgtag gggggatctg
    29221 gtaaagcgcc ggacgccaac aacccacgcc cggcatcgta attacgtatt gtctttcacc
    29281 gtctggcttg tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg
    29341 gcgtacatct ggctggagcc gataatttga tgattagcgg ctttcagatt gaaatagaat
    29401 ttgccgttgc ttgccgtttt ttctcatagc gctcttcttg cgggctgttg ctacgcaccg
    29461 acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg aggatagtct
    29521 caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa
    29581 accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc atct
//
LOCUS       DADLDT020000046        29236 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000046 DADLDT020000000
VERSION     DADLDT020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.046"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..218
                     /locus_tag="JQH22_001917"
     CDS             <1..218
                     /locus_tag="JQH22_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2206245.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="JQH22_001918"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="JQH22_001918"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFB2206246.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="JQH22_001919"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="JQH22_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFB2206247.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="JQH22_001920"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="JQH22_001920"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFB2206248.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="JQH22_001921"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="JQH22_001921"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFB2206249.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="JQH22_001922"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="JQH22_001922"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HFB2206250.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="JQH22_001923"
     CDS             7229..8179
                     /locus_tag="JQH22_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFB2206251.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="JQH22_001924"
     CDS             complement(8220..8690)
                     /locus_tag="JQH22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFB2206252.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="JQH22_001925"
     CDS             complement(8745..9947)
                     /locus_tag="JQH22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFB2206253.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="JQH22_001926"
     CDS             complement(10034..10576)
                     /locus_tag="JQH22_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206254.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="JQH22_001927"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="JQH22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFB2206255.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="JQH22_001928"
     CDS             complement(11319..11921)
                     /locus_tag="JQH22_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFB2206256.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="JQH22_001929"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="JQH22_001929"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFB2206257.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="JQH22_001930"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="JQH22_001930"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFB2206258.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="JQH22_001931"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="JQH22_001931"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFB2206259.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="JQH22_001932"
     CDS             16319..17209
                     /locus_tag="JQH22_001932"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFB2206260.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="JQH22_001933"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="JQH22_001933"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFB2206261.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKTIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="JQH22_001934"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="JQH22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFB2206262.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="JQH22_001935"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="JQH22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFB2206263.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="JQH22_001936"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="JQH22_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFB2206264.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="JQH22_001937"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="JQH22_001937"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFB2206265.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="JQH22_001938"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="JQH22_001938"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFB2206266.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="JQH22_001939"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="JQH22_001939"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFB2206267.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="JQH22_001940"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="JQH22_001940"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFB2206268.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="JQH22_001941"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="JQH22_001941"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFB2206269.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="JQH22_001942"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="JQH22_001942"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFB2206270.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="JQH22_001943"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="JQH22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFB2206271.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="JQH22_001944"
     CDS             complement(27384..27845)
                     /locus_tag="JQH22_001944"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2206272.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="JQH22_001945"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="JQH22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFB2206273.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="JQH22_001946"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="JQH22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7087 a         7856 c         7300 g         6993 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa aaccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DADLDT020000047        29048 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000047 DADLDT020000000
VERSION     DADLDT020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.047"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="JQH22_001947"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="JQH22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFB2206274.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="JQH22_001948"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="JQH22_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFB2206275.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="JQH22_001949"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="JQH22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFB2206276.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..5087
                     /locus_tag="JQH22_001950"
     CDS             4848..5087
                     /locus_tag="JQH22_001950"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206277.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5097..5267
                     /locus_tag="JQH22_001951"
     CDS             5097..5267
                     /locus_tag="JQH22_001951"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFB2206278.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5316..6728
                     /gene="fdhF"
                     /locus_tag="JQH22_001952"
     CDS             5316..6728
                     /gene="fdhF"
                     /locus_tag="JQH22_001952"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFB2206279.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6729..6995
                     /locus_tag="JQH22_001953"
     CDS             6729..6995
                     /locus_tag="JQH22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HFB2206280.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7089..7778
                     /locus_tag="JQH22_001954"
     CDS             7089..7778
                     /locus_tag="JQH22_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFB2206281.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="JQH22_001955"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="JQH22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFB2206282.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="JQH22_001956"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="JQH22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFB2206283.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="JQH22_001957"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="JQH22_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFB2206284.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="JQH22_001958"
     CDS             complement(10480..12198)
                     /locus_tag="JQH22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFB2206285.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="JQH22_001959"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="JQH22_001959"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFB2206286.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="JQH22_001960"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="JQH22_001960"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFB2206287.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="JQH22_001961"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="JQH22_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFB2206288.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="JQH22_001962"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="JQH22_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFB2206289.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="JQH22_001963"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="JQH22_001963"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFB2206290.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="JQH22_001964"
     CDS             18437..18751
                     /locus_tag="JQH22_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFB2206291.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="JQH22_001965"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="JQH22_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFB2206292.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="JQH22_001966"
     CDS             20575..21867
                     /locus_tag="JQH22_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFB2206293.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="JQH22_001967"
     CDS             complement(22021..23670)
                     /locus_tag="JQH22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFB2206294.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="JQH22_001968"
                     /gene_synonym="yjcD"
     CDS             complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="JQH22_001968"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFB2206295.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="JQH22_001969"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="JQH22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFB2206296.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="JQH22_001970"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="JQH22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFB2206297.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="JQH22_001971"
                     /pseudo
     CDS             complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="JQH22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28758..29039
                     /locus_tag="JQH22_001972"
     CDS             28758..29039
                     /locus_tag="JQH22_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFB2206298.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7777 c         7349 g         6634 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
    27481 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
    27541 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
    27601 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
    27661 tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca tttctttgta
    27721 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
    27781 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
    27841 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
    27901 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
    27961 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
    28021 agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa taatattcaa
    28081 cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
    28141 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
    28201 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
    28261 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
    28321 atgactcatt gctatcaatt tcctgcctgt ttatacccag ccattgcaag gtgaaaccct
    28381 ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa
    28441 ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca aaattaattt
    28501 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
    28561 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
    28621 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
    28681 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
    28741 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
    28801 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
    28861 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
    28921 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
    28981 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
    29041 acgtagaa
//
LOCUS       DADLDT020000048        28561 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000048 DADLDT020000000
VERSION     DADLDT020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28561
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.048"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..331)
                     /locus_tag="JQH22_001973"
     CDS             complement(<1..331)
                     /locus_tag="JQH22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206299.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="JQH22_001974"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="JQH22_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFB2206300.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="JQH22_001975"
     CDS             complement(1692..2072)
                     /locus_tag="JQH22_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFB2206301.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="JQH22_001976"
     CDS             complement(2086..2502)
                     /locus_tag="JQH22_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206302.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQH22_001977"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQH22_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFB2206303.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="JQH22_001978"
     CDS             complement(3503..3970)
                     /locus_tag="JQH22_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HFB2206304.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="JQH22_001979"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="JQH22_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFB2206305.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="JQH22_001980"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="JQH22_001980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFB2206306.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="JQH22_001981"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="JQH22_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFB2206307.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8323)
                     /gene="rhtB"
                     /locus_tag="JQH22_001982"
     CDS             complement(7712..8323)
                     /gene="rhtB"
                     /locus_tag="JQH22_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFB2206308.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQLMQYIVLGVTTIVVDIIVMI
                     GYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8434..9456
                     /gene="pldB"
                     /locus_tag="JQH22_001983"
     CDS             8434..9456
                     /gene="pldB"
                     /locus_tag="JQH22_001983"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFB2206309.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9464..10264
                     /gene="yigL"
                     /locus_tag="JQH22_001984"
     CDS             9464..10264
                     /gene="yigL"
                     /locus_tag="JQH22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFB2206310.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10340..11239
                     /gene="bioP"
                     /locus_tag="JQH22_001985"
     CDS             10340..11239
                     /gene="bioP"
                     /locus_tag="JQH22_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFB2206311.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11127..12080)
                     /gene="metR"
                     /locus_tag="JQH22_001986"
     CDS             complement(11127..12080)
                     /gene="metR"
                     /locus_tag="JQH22_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFB2206312.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12316..14577
                     /gene="metE"
                     /locus_tag="JQH22_001987"
     CDS             12316..14577
                     /gene="metE"
                     /locus_tag="JQH22_001987"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFB2206313.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14616..15431)
                     /locus_tag="JQH22_001988"
     CDS             complement(14616..15431)
                     /locus_tag="JQH22_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFB2206314.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15693..16454
                     /gene="udp"
                     /locus_tag="JQH22_001989"
     CDS             15693..16454
                     /gene="udp"
                     /locus_tag="JQH22_001989"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFB2206315.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16595..18022
                     /gene="rmuC"
                     /locus_tag="JQH22_001990"
     CDS             16595..18022
                     /gene="rmuC"
                     /locus_tag="JQH22_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFB2206316.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18117..18872
                     /gene="ubiE"
                     /locus_tag="JQH22_001991"
     CDS             18117..18872
                     /gene="ubiE"
                     /locus_tag="JQH22_001991"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFB2206317.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18886..19491
                     /gene="ubiJ"
                     /locus_tag="JQH22_001992"
     CDS             18886..19491
                     /gene="ubiJ"
                     /locus_tag="JQH22_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFB2206318.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19488..21128
                     /gene="ubiB"
                     /locus_tag="JQH22_001993"
     CDS             19488..21128
                     /gene="ubiB"
                     /locus_tag="JQH22_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFB2206319.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21207..21476
                     /gene="tatA"
                     /locus_tag="JQH22_001994"
     CDS             21207..21476
                     /gene="tatA"
                     /locus_tag="JQH22_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFB2206320.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21480..21995
                     /gene="tatB"
                     /locus_tag="JQH22_001995"
     CDS             21480..21995
                     /gene="tatB"
                     /locus_tag="JQH22_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HFB2206321.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21998..22774
                     /gene="tatC"
                     /locus_tag="JQH22_001996"
     CDS             21998..22774
                     /gene="tatC"
                     /locus_tag="JQH22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFB2206322.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22816..23598
                     /gene="tatD"
                     /locus_tag="JQH22_001997"
     CDS             22816..23598
                     /gene="tatD"
                     /locus_tag="JQH22_001997"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFB2206323.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="JQH22_001998"
     CDS             complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="JQH22_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFB2206324.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24250..25743
                     /gene="ubiD"
                     /locus_tag="JQH22_001999"
     CDS             24250..25743
                     /gene="ubiD"
                     /locus_tag="JQH22_001999"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFB2206325.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25789..26490
                     /gene="fre"
                     /locus_tag="JQH22_002000"
     CDS             25789..26490
                     /gene="fre"
                     /locus_tag="JQH22_002000"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFB2206326.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26682..27845)
                     /gene="fadA"
                     /locus_tag="JQH22_002001"
     CDS             complement(26682..27845)
                     /gene="fadA"
                     /locus_tag="JQH22_002001"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFB2206327.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27855..>28561)
                     /gene="fadB"
                     /locus_tag="JQH22_002002"
     CDS             complement(27855..>28561)
                     /gene="fadB"
                     /locus_tag="JQH22_002002"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /EC_number="5.3.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="includes enoyl-CoA hydratase,
                     delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase,
                     3-hydroxyacyl-CoA dehydrogenase, and 3-hydroxybutyryl-CoA
                     epimerase; catalyzes the formation of an hydroxyacyl-CoA
                     by addition of water on enoyl-CoA; also exhibits
                     3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA
                     dehydrogenase activities; forms a heterotetramer with
                     FadA; similar to FadI2J2 complex; functions in
                     beta-oxidation of fatty acids; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFB2206328.1"
                     /translation="GFFVNRVLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAY
                     LLDVVGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDANRFGQKNGLGFWRYKEDSK
                     GKPKKEEDAAVEDLLAEVSQPKRDFSEEEIIARMMIPMVNEVVRCLEEGIIATPAEAD
                     MALVYGLGFPPFHGGAFRWLDTLGSAKYLDMAQQYQHLGPLYEVPEGLRNKARHNEPY
                     YPPVEPARPVGDLKTA"
BASE COUNT         6779 a         7192 c         7705 g         6885 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggtaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg taccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccca ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca ggccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc atgatgaatt
     7921 gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc acaaaaactg
     7981 cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga tcaattgcgc
     8041 cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct gcccacttta
     8101 acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg ccaaccagca
     8161 caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg ccgcgataac
     8221 cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac aggcttaaaa
     8281 tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac tcccggtgtg
     8341 tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg cttaaaaaaa
     8401 cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa agactgggaa
     8461 acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt ctggcgtcag
     8521 cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt ccgttttcgc
     8581 gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag ctacgtaaaa
     8641 tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat catcgaccat
     8701 cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca tgttaatcgc
     8761 tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca gcccggtccg
     8821 tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac attatttctg
     8881 caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt tgggatcgtg
     8941 attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc gcatccacgt
     9001 ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt tgctatcaac
     9061 gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc tgatgaccca
     9121 acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct ggctggcgaa
     9181 caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca agctgaagag
     9241 gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac cgccgcgggc
     9301 catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga gatccttttt
     9361 gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt caacaggcat
     9421 aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc aggttgttgc
     9481 gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt acgccaaaga
     9541 aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg gtcgtcacca
     9601 cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga ttacctccaa
     9661 tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc tggatcgcga
     9721 cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta ctaacgttta
     9781 tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct tttttaaaga
     9841 agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag gcgtcagcaa
     9901 agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg cgattaacgc
     9961 tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg aagtgatggc
    10021 gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg gctacagcct
    10081 gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt cgatggcggg
    10141 gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc cggagctgga
    10201 agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac tctatttatc
    10261 gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg gatactcgtt
    10321 aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt gggccttctc
    10381 ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg cggtgctggt
    10441 gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta gcaatagcct
    10501 gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga tgtatatgct
    10561 gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca ccgtactgac
    10621 gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc gctggggcta
    10681 tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg atcaggtcac
    10741 cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt ttgccatcgg
    10801 catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata acgcctttgc
    10861 gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc tgggaaatgc
    10921 gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg gcgtggtggc
    10981 ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg ccggaacgct
    11041 gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg ctatctggca
    11101 ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg cctcactgtg
    11161 ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc gcaggcgtga
    11221 ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc atcgcgcacg
    11281 gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac cagaccctgg
    11341 cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg cgcggcaacc
    11401 atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac gcctgccggc
    11461 tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat taatagcgtc
    11521 tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg gtcaggtgct
    11581 aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact gcgcggcagg
    11641 atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg cgggtcaaac
    11701 gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa attttctaaa
    11761 gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag acgcgtctgc
    11821 tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg gtttgccagt
    11881 tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg cacaaatagc
    11941 cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga ttgcgtctga
    12001 tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg tagcgttttc
    12061 aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt gcgcttgagg
    12121 aatatacagt aaccgctaat tatggatgtg taaacatctg gacggctaaa atcattcgtc
    12181 ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact tcagtaagct
    12241 cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga tttacatata
    12301 attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg cgttggcctg
    12361 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
    12421 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
    12481 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac caccagtttg
    12541 ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
    12601 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    12661 accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa aggccaacag
    12721 ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc gctgagccac
    12781 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    12841 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    12901 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    12961 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    13021 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    13081 gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa cgttgttgaa
    13141 ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa tggtcgtaac
    13201 gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    13261 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    13321 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    13381 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    13441 gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt
    13501 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa
    13561 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    13621 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac
    13681 aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg
    13741 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    13801 gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    13861 tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat taccgtggag
    13921 tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct gacggggccg
    13981 gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    14041 cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg aattggcatc
    14101 atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat
    14161 gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    14221 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    14281 gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    14341 tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga cattcactcg
    14401 ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc aaaacgcatt
    14461 ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggccagaa
    14521 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg ggggtaaatc
    14581 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    14641 tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    14701 gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat ctctgctttc
    14761 gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg aatgctgtta
    14821 tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    14881 tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc cactgcggct
    14941 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    15001 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    15061 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    15121 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    15181 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    15241 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    15301 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    15361 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    15421 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    15481 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    15541 agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    15601 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    15661 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    15721 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    15781 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    15841 tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    15901 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    15961 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    16021 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    16081 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct attggcgcga
    16141 caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag gaacgttacg
    16201 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    16261 tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt gcaagtcagg
    16321 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    16381 atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc
    16441 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    16501 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    16561 tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca tggtttacgc
    16621 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aacatgcgca
    16681 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    16741 acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca ataacgaagt
    16801 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    16861 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    16921 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    16981 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    17041 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    17101 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    17161 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    17221 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    17281 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    17341 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    17401 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    17461 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    17521 ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg accgccagcc
    17581 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    17641 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gtaacgccca
    17701 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    17761 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    17821 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    17881 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    17941 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    18001 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    18061 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    18121 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    18181 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    18241 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    18301 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    18361 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    18421 aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    18481 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    18541 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    18601 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    18661 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    18721 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    18781 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcagggg
    18841 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    18901 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    18961 aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    19021 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    19081 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    19141 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    19201 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    19261 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    19321 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    19381 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    19441 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    19501 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    19561 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    19621 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    19681 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    19741 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    19801 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    19861 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    19921 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gtcattaaag
    19981 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    20041 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt gatgaactga
    20101 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    20161 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    20221 gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc actaacatga
    20281 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    20341 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    20401 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    20461 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    20521 tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    20581 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    20641 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    20701 tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg cagctcgatt
    20761 tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    20821 ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    20881 ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    20941 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    21001 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gagtgggggc
    21061 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    21121 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa
    21181 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    21241 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    21301 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    21361 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    21421 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    21481 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    21541 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    21601 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    21661 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt
    21721 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    21781 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    21841 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    21901 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    21961 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    22021 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    22081 cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg tatccgcgcc
    22141 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    22201 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    22261 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    22321 gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg cctactttgt
    22381 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    22441 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    22501 ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct cgtcagaaga
    22561 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    22621 gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc tgtttgaaat
    22681 cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    22741 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    22801 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    22861 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    22921 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    22981 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    23041 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    23101 tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc tcagctacgc
    23161 attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    23221 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    23281 ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg cattaccggt
    23341 tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt gattccggcg
    23401 gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct cacgccaaag
    23461 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    23521 tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt caaaacactg
    23581 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    23641 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    23701 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataagggggg
    23761 gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac cgccgatggg
    23821 actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt agcgctgatg
    23881 gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg gaataacggt
    23941 tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat cggagcgagg
    24001 caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag
    24061 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    24121 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    24181 gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg cgcagattaa
    24241 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac gctgcttgaa
    24301 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    24361 attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    24421 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    24481 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    24541 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaggtg
    24601 ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    24661 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    24721 ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca gaatctgggc
    24781 atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    24841 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca tttcccggtt
    24901 tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc cgttccggat
    24961 acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga agtagtaaag
    25021 tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga agggtatatc
    25081 gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta ctataatgaa
    25141 gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    25201 cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt ggcgttgaac
    25261 gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    25321 ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta cgccggacac
    25381 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaaattt
    25441 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    25501 accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    25561 ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga tgccacgaat
    25621 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    25681 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    25741 tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    25801 agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt ccgcatcgtg
    25861 ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat ggatgagcgc
    25921 gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat cgagctgcat
    25981 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    26041 catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    26101 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    26161 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    26221 catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg tctgcaagtg
    26281 gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac cgtgttaacg
    26341 gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc cggacgtttt
    26401 gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc
    26461 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    26521 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatt
    26581 ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgccggatc
    26641 ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc
    26701 gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg
    26761 cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc cagcggatga
    26821 cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc cattagcccg
    26881 agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg
    26941 atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg
    27001 taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg agcgcgcggc
    27061 ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg
    27121 gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa cgccggacgc
    27181 agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc
    27241 ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt aaatgcggcc
    27301 gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca
    27361 cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct
    27421 ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg
    27481 cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat gatcattcgt
    27541 gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg
    27601 acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa accctgctcc
    27661 agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg
    27721 ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg cacgttacga
    27781 aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt
    27841 tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccgga
    27901 ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc
    27961 gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc
    28021 agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac cagcgccata
    28081 tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac ttcgttgacc
    28141 atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt cggttggctc
    28201 acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt acctttgctg
    28261 tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac
    28321 agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc tgctgccatg
    28381 acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata tgccgggccc
    28441 atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg
    28501 cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac aaagaagccg
    28561 g
//
LOCUS       DADLDT020000049        28431 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000049 DADLDT020000000
VERSION     DADLDT020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28431
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.049"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            564..1334
                     /locus_tag="JQH22_002003"
     CDS             564..1334
                     /locus_tag="JQH22_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFB2206329.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JQH22_002004"
     CDS             1325..1798
                     /locus_tag="JQH22_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFB2206330.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JQH22_002005"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JQH22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFB2206331.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JQH22_002006"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JQH22_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFB2206332.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JQH22_002007"
     CDS             3233..3727
                     /locus_tag="JQH22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HFB2206333.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JQH22_002008"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JQH22_002008"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFB2206334.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="JQH22_002009"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="JQH22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="JQH22_002010"
     CDS             7571..8485
                     /locus_tag="JQH22_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFB2206335.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="JQH22_002011"
     CDS             8700..10061
                     /locus_tag="JQH22_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206336.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JQH22_002012"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JQH22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFB2206337.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JQH22_002013"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JQH22_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFB2206338.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="JQH22_002014"
     CDS             12611..13900
                     /locus_tag="JQH22_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFB2206339.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="JQH22_002015"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="JQH22_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFB2206340.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="JQH22_002016"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="JQH22_002016"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFB2206341.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="JQH22_002017"
     CDS             16226..16606
                     /locus_tag="JQH22_002017"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFB2206342.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="JQH22_002018"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="JQH22_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HFB2206343.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="JQH22_002019"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="JQH22_002019"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFB2206344.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="JQH22_002020"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="JQH22_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFB2206345.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="JQH22_002021"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="JQH22_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFB2206346.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="JQH22_002022"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="JQH22_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="JQH22_002023"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="JQH22_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="JQH22_002024"
     CDS             23335..23847
                     /locus_tag="JQH22_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFB2206347.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="JQH22_002025"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="JQH22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFB2206348.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="JQH22_002026"
     CDS             26440..26643
                     /locus_tag="JQH22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFB2206349.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="JQH22_002027"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="JQH22_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFB2206350.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28431)
                     /locus_tag="JQH22_002028"
     CDS             complement(27679..>28431)
                     /locus_tag="JQH22_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFB2206351.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6959 a         7432 c         7127 g         6913 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag t
//
LOCUS       DADLDT020000050        28403 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000050 DADLDT020000000
VERSION     DADLDT020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28403
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.050"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JQH22_002029"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JQH22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFB2206352.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="JQH22_002030"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="JQH22_002030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HFB2206353.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="JQH22_002031"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="JQH22_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFB2206354.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="JQH22_002032"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="JQH22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFB2206355.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="JQH22_002033"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="JQH22_002033"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFB2206356.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="JQH22_002034"
     CDS             6426..7055
                     /locus_tag="JQH22_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFB2206357.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="JQH22_002035"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="JQH22_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFB2206358.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8730..10073)
                     /gene="melA"
                     /locus_tag="JQH22_002036"
     CDS             complement(8730..10073)
                     /gene="melA"
                     /locus_tag="JQH22_002036"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFB2206359.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="JQH22_002037"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="JQH22_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFB2206360.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="JQH22_002038"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="JQH22_002038"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFB2206361.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="JQH22_002039"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="JQH22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFB2206362.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="JQH22_002040"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="JQH22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFB2206363.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="JQH22_002041"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="JQH22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFB2206364.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="JQH22_002042"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="JQH22_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFB2206365.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="JQH22_002043"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="JQH22_002043"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFB2206366.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="JQH22_002044"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="JQH22_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFB2206367.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="JQH22_002045"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="JQH22_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFB2206368.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="JQH22_002046"
     CDS             complement(21746..22624)
                     /locus_tag="JQH22_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFB2206369.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="JQH22_002047"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="JQH22_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFB2206370.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25251..25586
                     /locus_tag="JQH22_002048"
     CDS             25251..25586
                     /locus_tag="JQH22_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HFB2206371.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25744..26187
                     /gene="yjdN"
                     /locus_tag="JQH22_002049"
     CDS             25744..26187
                     /gene="yjdN"
                     /locus_tag="JQH22_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFB2206372.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26320..27108
                     /gene="phnC"
                     /locus_tag="JQH22_002050"
     CDS             26320..27108
                     /gene="phnC"
                     /locus_tag="JQH22_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFB2206373.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27133..28149
                     /gene="phnD"
                     /locus_tag="JQH22_002051"
     CDS             27133..28149
                     /gene="phnD"
                     /locus_tag="JQH22_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2206374.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28255..>28403
                     /locus_tag="JQH22_002052"
     CDS             28255..>28403
                     /locus_tag="JQH22_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFB2206375.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7421 c         6935 g         6752 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agcactgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctgacagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac tagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgt atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 cgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgca actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc attagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgtttttta tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa
    25021 atatcatgtg aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata
    25081 gaggcaaaaa gattattttc tttttacgtt tcctttcaaa tgaaaacgat cgtcgtctaa
    25141 aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca
    25201 atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac
    25261 cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc
    25321 cggaatgtgc ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg
    25381 ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc
    25441 tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc
    25501 tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga
    25561 aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc
    25621 gcgccgcatc cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca
    25681 gcctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    25741 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    25801 tatcaacgta cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa
    25861 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    25921 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca
    25981 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    26041 aaacgctggt ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    26101 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    26161 gtcgtcaaac aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat
    26221 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    26281 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    26341 gagaagctcg ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt
    26401 catcacagtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    26461 cgtcatttaa gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg
    26521 ggccgcactg tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac
    26581 accggctaca tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    26641 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    26701 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    26761 cagcgcgttt ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg
    26821 atgcagcagg cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca
    26881 gcgcgcatcg tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc
    26941 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt
    27001 caggggcacg tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat
    27061 ctctaccgca gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat
    27121 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    27181 ctcagcaccc tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    27241 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat
    27301 atggagaaga aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt
    27361 atccagggga tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg
    27421 gaagcggtgg atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg
    27481 ccgggttact ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat
    27541 ctgctggcga agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc
    27601 ttcctcgtcc ccggttacta cgtcttcgct aaaaacaata tctccgccag cgacttcaag
    27661 cgcaccgtca acgccgggca tgaaaccaac gcgctggccg tcgccaacaa gcaagtggat
    27721 gttgccacca acaacaccga aaacctcgac aagctgaaaa cctccgcgcc ggagaagctt
    27781 aaagagctga aggtgatctg gaaatcgccg ctgatcctag gcgatccgat cgtctggcgc
    27841 aagaatctgt ccgaaactac caaagacaag atctacgact tctttatgaa ctacggaaaa
    27901 acgccggaag agaaagcggt gctggaacgc ctgggctggg cgccgttccg cgcttccagc
    27961 gacctgcaac tggtgccgat tcgccagctc gcactgttta aagagataca aagcgtgaaa
    28021 ggcaataaag ggctgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag
    28081 ctggatgacc tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg
    28141 gtgcagtaaa tcgtaggtcg gataagacgc cccggcgtcg catccgacaa tgtgtaggcg
    28201 ttgatgccgg atgcggtgca agcaccttat ccggcctaca gaccggagcc aaacatgcaa
    28261 accatcacca tcgccccacc caagcgtagc tggttctcgc ttctgagctg ggccgttgtt
    28321 ctcgacgtgc tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgattaaa
    28381 gacggcggca acatggcaac ctt
//
LOCUS       DADLDT020000051        28338 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000051 DADLDT020000000
VERSION     DADLDT020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.051"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQH22_002053"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQH22_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="JQH22_002054"
     CDS             complement(998..2020)
                     /locus_tag="JQH22_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2206376.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JQH22_002055"
     CDS             complement(2034..3536)
                     /locus_tag="JQH22_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFB2206377.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQH22_002056"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQH22_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="JQH22_002057"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="JQH22_002057"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFB2206378.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="JQH22_002058"
     CDS             complement(5851..6192)
                     /locus_tag="JQH22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFB2206379.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JQH22_002059"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JQH22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFB2206380.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JQH22_002060"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JQH22_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFB2206381.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="JQH22_002061"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="JQH22_002061"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFB2206382.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="JQH22_002062"
     CDS             12871..14214
                     /locus_tag="JQH22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFB2206383.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="JQH22_002063"
     CDS             complement(14276..14482)
                     /locus_tag="JQH22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFB2206384.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="JQH22_002064"
     CDS             14807..15361
                     /locus_tag="JQH22_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFB2206385.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="JQH22_002065"
     CDS             complement(15424..16362)
                     /locus_tag="JQH22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206386.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="JQH22_002066"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="JQH22_002066"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFB2206387.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="JQH22_002067"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="JQH22_002067"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFB2206388.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="JQH22_002068"
     CDS             complement(19573..19953)
                     /locus_tag="JQH22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFB2206389.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="JQH22_002069"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="JQH22_002069"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFB2206390.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="JQH22_002070"
     CDS             21011..21976
                     /locus_tag="JQH22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFB2206391.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="JQH22_002071"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="JQH22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFB2206392.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="JQH22_002072"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="JQH22_002072"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFB2206393.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="JQH22_002073"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="JQH22_002073"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFB2206394.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="JQH22_002074"
     CDS             25350..25895
                     /locus_tag="JQH22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFB2206395.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="JQH22_002075"
     CDS             complement(25879..26526)
                     /locus_tag="JQH22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFB2206396.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="JQH22_002076"
     CDS             26738..27529
                     /locus_tag="JQH22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFB2206397.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="JQH22_002077"
     CDS             27539..>28338
                     /locus_tag="JQH22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HFB2206398.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7542 c         6989 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DADLDT020000052        27556 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000052 DADLDT020000000
VERSION     DADLDT020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27556
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.052"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JQH22_002078"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JQH22_002078"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFB2206399.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JQH22_002079"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JQH22_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFB2206400.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="JQH22_002080"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="JQH22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HFB2206401.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="JQH22_002081"
     CDS             3807..3995
                     /locus_tag="JQH22_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HFB2206402.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="JQH22_002082"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="JQH22_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFB2206403.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="JQH22_002083"
     CDS             4756..5115
                     /locus_tag="JQH22_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFB2206404.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="JQH22_002084"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="JQH22_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="JQH22_002085"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="JQH22_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFB2206405.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="JQH22_002086"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="JQH22_002086"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HFB2206406.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10895..11071
                     /locus_tag="JQH22_002087"
                     /pseudo
     CDS             10895..11071
                     /locus_tag="JQH22_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11399..13147
                     /gene="bcsC"
                     /locus_tag="JQH22_002088"
                     /pseudo
     CDS             11399..13147
                     /gene="bcsC"
                     /locus_tag="JQH22_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13229..15217
                     /gene="hmsP"
                     /locus_tag="JQH22_002089"
     CDS             13229..15217
                     /gene="hmsP"
                     /locus_tag="JQH22_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFB2206407.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15402..16688
                     /gene="dctA"
                     /locus_tag="JQH22_002090"
     CDS             15402..16688
                     /gene="dctA"
                     /locus_tag="JQH22_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFB2206408.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16909..18405
                     /locus_tag="JQH22_002091"
     CDS             16909..18405
                     /locus_tag="JQH22_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HFB2206409.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="JQH22_002092"
     CDS             complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="JQH22_002092"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFB2206410.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19662..20429
                     /gene="pdeH"
                     /locus_tag="JQH22_002093"
     CDS             19662..20429
                     /gene="pdeH"
                     /locus_tag="JQH22_002093"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFB2206411.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20499..22559
                     /locus_tag="JQH22_002094"
     CDS             20499..22559
                     /locus_tag="JQH22_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFB2206412.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22741..24063)
                     /locus_tag="JQH22_002095"
     CDS             complement(22741..24063)
                     /locus_tag="JQH22_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HFB2206413.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="JQH22_002096"
     CDS             complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="JQH22_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFB2206414.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25536..26435)
                     /locus_tag="JQH22_002097"
     CDS             complement(25536..26435)
                     /locus_tag="JQH22_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2206415.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26955..>27556
                     /locus_tag="JQH22_002098"
     CDS             26955..>27556
                     /locus_tag="JQH22_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFB2206416.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVQVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6565 a         6952 c         7551 g         6488 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccaag
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaataatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttattgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgtgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caactctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtagtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac
    11221 gttaatgcgc aatctggcgc agcagaagcc gaacaacccg gagcaggttt acgcttacgg
    11281 gctgtacctc tcaggtcaca accaggacag agcggcgctg gcgcatatca acagcctgcc
    11341 gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt
    11401 gctggaaacc gctaatcgcc tgcgagaaag cggcaaagag gctgaagcgg aagcgatgct
    11461 gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg cgtaacaacg
    11521 acgtgattac accgccgccc gcactgcata tcagaatgtc ctgacgcggg agccaactaa
    11581 cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc
    11641 acgtagccag ctggcgaaac tgcctgccac cgataacacc tcgctgaaca ctcagcggcg
    11701 cgtggcgctg gcacaggcgc agcttggaga taccgcagcg gcgcagcaga cgtttaataa
    11761 gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tactgcgcga
    11821 cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa cctacaaaga
    11881 cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg
    11941 actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc
    12001 ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag
    12061 cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc
    12121 gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt
    12181 ctccactaat gccgatggca aatgggatga caactggggc acctgtacat tacaggactg
    12241 tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga
    12301 cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg
    12361 cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg
    12421 gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg
    12481 gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg
    12541 tgaagtaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga
    12601 agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa
    12661 tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg
    12721 ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc
    12781 ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg
    12841 gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga
    12901 ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta
    12961 cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg gtacggcaat
    13021 tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg tacgttattc
    13081 cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga
    13141 ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg
    13201 caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca
    13261 gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct
    13321 gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg
    13381 ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc
    13441 tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa
    13501 ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg taatggttac
    13561 tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc
    13621 aaatccgcag ccaatagcct atctggtgct acaggcggat tccttccgta tgtataagtt
    13681 cgtgatgagc actctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac
    13741 cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg
    13801 cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct
    13861 gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct
    13921 gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc
    13981 gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc
    14041 gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca
    14101 acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat
    14161 gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc
    14221 gtggcacgca atcactttgg gtcagcaagt actcactatc atgagcgagc gcctgccgat
    14281 tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct
    14341 caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg
    14401 caagaatcag attcagttct ttgatccgca gcagatggaa gcagcccaga agcggttgac
    14461 ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt ggttacagcc
    14521 acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca
    14581 accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct
    14641 gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca
    14701 agagcgcggc attatgctgc ccttgtcggt aaacttctct gcgctgcaac tgatgcaccc
    14761 gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact
    14821 gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg tggcaatcct
    14881 ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc
    14941 agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa
    15001 aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc
    15061 gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac aacgagactg
    15121 gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat
    15181 tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac
    15241 tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta
    15301 tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtaccccc
    15361 cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta
    15421 aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct
    15481 atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga
    15541 tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca
    15601 tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca
    15661 tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg
    15721 tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc
    15781 agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca
    15841 gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc
    15901 tgggcagcaa aggccaactg atttttaacg tgatcgaaag tttctcgcag gtcatcttcg
    15961 gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca atggcgttta
    16021 ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct
    16081 acattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt
    16141 tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt
    16201 catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta
    16261 aatcggtggt ggggctggtt atcccgacag gctactcgtt taaccttgat ggcacatcga
    16321 tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg
    16381 tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa
    16441 cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag
    16501 cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctaacta
    16561 acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc
    16621 acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat
    16681 cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata
    16741 attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg
    16801 tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc
    16861 gacaacgggc agttttttta ttaccaggat agttgatcag gggttcacat gcagggcata
    16921 aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca
    16981 gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg
    17041 caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat
    17101 accggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt
    17161 gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg
    17221 atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat
    17281 ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat
    17341 gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac
    17401 atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg
    17461 tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa
    17521 attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga
    17581 aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc
    17641 aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt
    17701 attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag
    17761 ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg
    17821 ctgttctggc atgttcagca agcgttaagc gccagtgaca gcaaagacat cggtcttgga
    17881 tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac
    17941 gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa
    18001 ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa
    18061 ctgtttgccg cctatgcacg ggctgatacc gatattctga tgggccagcg gatgcgttcg
    18121 ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc
    18181 cttaacagcc tgactgtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat
    18241 atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag
    18301 gcggcctggg atcaaatcat ggccccatct accgccgctg cgaccacctc tgtcgccacg
    18361 gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg
    18421 agttagcaca accatcaggt atttatggat gagctggata gaggggtaac actttatcca
    18481 gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga
    18541 tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg
    18601 cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg
    18661 atcacttttt ctttcggcag tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg
    18721 gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg
    18781 gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc
    18841 gtcaggaagg cgatatccgt gcattccagc atttgctggt acacctgctg tgtctcttct
    18901 ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg
    18961 cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg
    19021 ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac
    19081 tgctcgctcg ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt
    19141 tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa gacggttttc catccgttgg
    19201 gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg
    19261 tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg
    19321 atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc
    19381 tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac
    19441 ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta
    19501 aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag
    19561 tgaggaaatt taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt
    19621 cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc
    19681 agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc
    19741 agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg
    19801 tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc
    19861 gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt
    19921 tgctggcgca aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg
    19981 atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc
    20041 ttccctggct gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg
    20101 cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt
    20161 tctctgcact aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga
    20221 tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat ctaatgaatc
    20281 gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc
    20341 agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatggaaa
    20401 cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgccggg ctggactatc
    20461 tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa
    20521 ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa
    20581 cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc
    20641 gtccgttcgc tatccgtggc aatctgggcg tggtgtggga gcggcaaaaa caagaaactg
    20701 gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt cttggtaatc
    20761 caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca acgctggcac
    20821 cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg
    20881 cgcgcctgat tcgcctctct gaaaagaaca ataactggac gtttaatctt gccaacgatg
    20941 acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt
    21001 tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg
    21061 ttgatccgtt aggcaagccg ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg
    21121 ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc gcagggacgt tataacggtg
    21181 aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc
    21241 cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt gatggcgtcg
    21301 tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac
    21361 tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg tttgaaacgg
    21421 atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt
    21481 ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc
    21541 cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac
    21601 cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga
    21661 agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg
    21721 ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca
    21781 gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc
    21841 tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt catctggaag
    21901 gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag
    21961 aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg
    22021 aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga
    22081 aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg
    22141 tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg
    22201 cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg
    22261 agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga
    22321 ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc
    22381 gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt gcccgtggtg
    22441 ctgttgctgc ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct
    22501 caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat
    22561 gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt ggcaaagtgt
    22621 gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt
    22681 caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt ttcacactta
    22741 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    22801 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    22861 gatatacggc gcaacggaag ccccgagaat cgacgatacg ttgtaagaga acgatgctcc
    22921 ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc gcacccatcg gcccgaaggt
    22981 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    23041 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac
    23101 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    23161 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    23221 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    23281 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    23341 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    23401 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    23461 tggcacacgc cagccccagc tcataaactg ctcatcggtc agtagccagg aaagcagcaa
    23521 gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct agctgtggga aggagccata
    23581 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcac cgccccattc
    23641 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat
    23701 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    23761 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    23821 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    23881 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    23941 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    24001 attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg
    24061 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    24121 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    24181 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    24241 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggag
    24301 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    24361 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    24421 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    24481 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    24541 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    24601 aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat cacgatttta
    24661 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    24721 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    24781 atcgccaggc caatcaatcg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    24841 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc
    24901 agcgcaatca acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    24961 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    25021 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    25081 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga
    25141 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    25201 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    25261 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    25321 cgaataaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    25381 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    25441 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    25501 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    25561 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    25621 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    25681 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    25741 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    25801 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    25861 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    25921 agatagtgcg gactggcaca gttgaccatg gttagtttgc cgaggggacg ggcgataacg
    25981 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    26041 accggacggt tatggctact cagttccagc tcaattcccg gatgctgata gagaaattcc
    26101 gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat gcgtaattta
    26161 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    26221 acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta
    26281 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    26341 cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    26401 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    26461 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    26521 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    26581 atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct ttaaagtgaa
    26641 tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt tattttggct
    26701 tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    26761 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    26821 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    26881 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    26941 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    27001 taaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggtc agtcaggcag
    27061 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    27121 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggttc
    27181 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    27241 atgtgctggc cattgtgcct cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata
    27301 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    27361 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    27421 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    27481 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    27541 taaatatctc ctcctg
//
LOCUS       DADLDT020000053        27537 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000053 DADLDT020000000
VERSION     DADLDT020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27537
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.053"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JQH22_002099"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JQH22_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFB2206417.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="JQH22_002100"
     CDS             complement(1614..1937)
                     /locus_tag="JQH22_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFB2206418.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQH22_002101"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQH22_002101"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFB2206419.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQH22_002102"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQH22_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFB2206420.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="JQH22_002103"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="JQH22_002103"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFB2206421.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQH22_002104"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQH22_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFB2206422.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JQH22_002105"
     CDS             7537..8574
                     /locus_tag="JQH22_002105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFB2206423.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JQH22_002106"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JQH22_002106"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFB2206424.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JQH22_002107"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JQH22_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFB2206425.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JQH22_002108"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JQH22_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFB2206426.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JQH22_002109"
     CDS             10745..11626
                     /locus_tag="JQH22_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2206427.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JQH22_002110"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JQH22_002110"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFB2206428.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JQH22_002111"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JQH22_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFB2206429.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JQH22_002112"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JQH22_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JQH22_002113"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JQH22_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFB2206430.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JQH22_002114"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JQH22_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFB2206431.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JQH22_002115"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JQH22_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFB2206432.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JQH22_002116"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JQH22_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFB2206433.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JQH22_002117"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JQH22_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFB2206434.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JQH22_002118"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JQH22_002118"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HFB2206435.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JQH22_002119"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JQH22_002119"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFB2206436.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JQH22_002120"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JQH22_002120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFB2206437.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JQH22_002121"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JQH22_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFB2206438.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JQH22_002122"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JQH22_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFB2206439.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JQH22_002123"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JQH22_002123"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFB2206440.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JQH22_002124"
     CDS             24893..25273
                     /locus_tag="JQH22_002124"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFB2206441.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JQH22_002125"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JQH22_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFB2206442.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JQH22_002126"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JQH22_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFB2206443.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27537
                     /locus_tag="JQH22_002127"
                     /pseudo
     CDS             26442..27537
                     /locus_tag="JQH22_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6796 a         6855 c         7219 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaa
//
LOCUS       DADLDT020000054        27493 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000054 DADLDT020000000
VERSION     DADLDT020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27493
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.054"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(24..278)
                     /locus_tag="JQH22_002128"
     CDS             complement(24..278)
                     /locus_tag="JQH22_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFB2206444.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JQH22_002129"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JQH22_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFB2206445.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JQH22_002130"
     CDS             1228..2694
                     /locus_tag="JQH22_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFB2206446.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="JQH22_002131"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="JQH22_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFB2206447.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JQH22_002132"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JQH22_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFB2206448.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JQH22_002133"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JQH22_002133"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFB2206449.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JQH22_002134"
     CDS             5709..7265
                     /locus_tag="JQH22_002134"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFB2206450.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JQH22_002135"
     CDS             7347..9161
                     /locus_tag="JQH22_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFB2206451.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JQH22_002136"
     CDS             9162..10256
                     /locus_tag="JQH22_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFB2206452.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JQH22_002137"
     CDS             10256..11281
                     /locus_tag="JQH22_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2206453.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JQH22_002138"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JQH22_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFB2206454.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JQH22_002139"
     CDS             complement(12876..13220)
                     /locus_tag="JQH22_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206455.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQH22_002140"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQH22_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFB2206456.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQH22_002141"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQH22_002141"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFB2206457.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JQH22_002142"
     CDS             15616..17376
                     /locus_tag="JQH22_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFB2206458.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JQH22_002143"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JQH22_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFB2206459.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQH22_002144"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQH22_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFB2206460.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JQH22_002145"
     CDS             19113..19340
                     /locus_tag="JQH22_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFB2206461.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JQH22_002146"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JQH22_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HFB2206462.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JQH22_002147"
     tRNA            21195..21271
                     /locus_tag="JQH22_002147"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            21478..22722
                     /locus_tag="JQH22_002148"
     CDS             21478..22722
                     /locus_tag="JQH22_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2206463.1"
                     /translation="MARKATPLTTTQIKAAKPAEKEYTLQDGGGLFLVIKPSGSKLWR
                     FNYYRPSNKKRTLISLGSLDEVSLADARKRRSEYRTLIAAGTDPQEYERQKREAEARR
                     QGNTFENVAALWYEMKKNQNLAHNTIKDIWRSLEKYVFPYIGNTPIDTLTARRFVEIL
                     TPIKARGNLETLKRVLQRINEVMDFAANSGMIDINTAANVRKTFPSPTKKHMPTIRPE
                     QLPQLMHDLSIASIERQTRLLIEWQLLTATRPAEASAARWEEINLEAATWTIPAGRMK
                     MRRDHVIPLCAQAMAVLEAMKPISARREHVFPSLKNPVQPMSSQTANAALRRMGYTGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYLEKRKVLMCWWGD
                     FVEAAATGTTIASGHRGLRGIK"
     gene            22997..23188
                     /locus_tag="JQH22_002149"
     CDS             22997..23188
                     /locus_tag="JQH22_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HFB2206464.1"
                     /translation="MRAILLRPEVKALLRIKSDSTLTKMVNKGGFPKGFRVGLRRIGW
                     YEDEVLAWLKERERESREA"
     gene            23415..23858
                     /locus_tag="JQH22_002150"
                     /pseudo
     CDS             23415..23858
                     /locus_tag="JQH22_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            23834..24022
                     /locus_tag="JQH22_002151"
     CDS             23834..24022
                     /locus_tag="JQH22_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFB2206465.1"
                     /translation="MATIPALAHTQNAFIWRFIIVNPSDYRVVHVVASTEREARNYCP
                     SGCVAVFAARIRQGVSHA"
     gene            24015..24200
                     /locus_tag="JQH22_002152"
     CDS             24015..24200
                     /locus_tag="JQH22_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206466.1"
                     /translation="MLKRFQITARAVNHQGHVIDIRQNVNATSVNAAIAILKEELIGC
                     GFTQVMIRAVVEVSTVH"
     gene            24240..24539
                     /locus_tag="JQH22_002153"
     CDS             24240..24539
                     /locus_tag="JQH22_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFB2206467.1"
                     /translation="MKVKNSGFTASGPVRPEIHTGDIFFDKYGWMVKVISVEERRITC
                     RREGYNYDCVTPVYQFRRDFSLMKAAPRSKPTSREKERGNIQKMKSMINTFRGKK"
     gene            24536..26671
                     /locus_tag="JQH22_002154"
     CDS             24536..26671
                     /locus_tag="JQH22_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFB2206468.1"
                     /translation="MKRAPNLKHQPADKMTEVIIFAGSDAWAHAKQWQEMDGRLAGDN
                     VPPVWLGDQQLAELENLNIIDDGRYCVRLYKAGHIKPSNINTIAQKLAAAGIRDANYY
                     PDGMHSQKRENWREYLERERQNIDDGLAIRLPTKENATGSHGDDELKPRVESRADGVF
                     WVTPKVDKQTGEIIRPETWLCSPLELLGTGTIGREHHRVMRWKKTANHEVITMAVPCG
                     GIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGNHEEWQLSTTTGWHFGAYIMPD
                     GSVIGESEKPILFTGKTAAVNGYSVAGTTEGWRDTVARLAGGNPSMMLGVAVSLSAPL
                     IGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHND
                     GLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEMDVE
                     TFLKSEGIKVKAGQLVRLLNVPMEKSTQFHEYSTGKAHADALKDAWTANHGAAGREWI
                     KWLAAHQQKAKDTVRACRERWRNLIPESYGEQVHRVGERFAILEAALVLSGNVTGWDV
                     QACRDAIQHNFNAWVKEFGTGNKEHRQIIEQAEAFLAAYGMSRFAPVNYDPASLPISE
                     LYGYRESDGRYGESVLFYVLPEPFKSHVAKGFNKDAVARVLHEAGMLKKPASGEGWQI
                     RTPRLKHMKGARLRVYGLLLAQDHDDEGG"
     gene            26908..27117
                     /locus_tag="JQH22_002155"
     CDS             26908..27117
                     /locus_tag="JQH22_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFB2206469.1"
                     /translation="MTAQISAYGRLVADPQLKTTSKGTPMALVSMAVPIPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHCKYPA"
     gene            27179..27493
                     /locus_tag="JQH22_002156"
                     /pseudo
     CDS             27179..27493
                     /locus_tag="JQH22_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6992 a         6833 c         6945 g         6723 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaatcaacta tagctaatca tcgtttttat
    21301 gaaaaaagcc cgctaattca ttgaatagcg ggttttttta ttgtttttag tcatcgttat
    21361 tcgacggtaa cagtggtttt cagccgtttt ttttagttat atgtttagtt atacgggctg
    21421 gcgcatagct aaatccgtat aactagaacc tgtttttccc cgtggattca taaacgtatg
    21481 gcaagaaagg caaccccact caccaccaca cagatcaaag cagctaaacc agcagaaaaa
    21541 gaatacacct tacaggatgg cggcgggctt tttctcgtta ttaagccgtc tggttcgaaa
    21601 ctatggcgat tcaactacta tcgaccttcg aacaaaaaac gaacactcat tagtttggga
    21661 tcgcttgatg aagtctccct tgctgatgcc agaaaacgcc gtagcgagta caggacgtta
    21721 attgcggcag gaaccgaccc gcaggaatac gaacggcaaa aacgcgaagc agaggctcga
    21781 cgacagggca acacgttcga aaatgtggcc gcattgtggt acgaaatgaa aaaaaaccag
    21841 aatctcgccc acaatacgat caaggacatc tggcgctcgc tagagaaata tgtattcccg
    21901 tatatcggaa acacaccgat agatactctc accgctcgcc gctttgttga gatactcacg
    21961 cccatcaagg cacgtggcaa cctggaaaca ctgaaacgcg ttttacagcg catcaatgaa
    22021 gtgatggatt ttgctgccaa cagtggaatg attgacatca acaccgccgc gaacgttcgc
    22081 aagacattcc cttctcccac caaaaagcac atgccaacca tccggccgga acaactaccg
    22141 cagctaatgc acgatttatc gatcgccagc atagaacggc aaaccagatt gctgattgag
    22201 tggcagttgt taaccgcaac ccgcccagcc gaagcctctg ccgcacggtg ggaagaaatc
    22261 aaccttgagg cggcaacctg gacgatacca gccggacgca tgaagatgcg ccgtgatcat
    22321 gtgatccccc tttgcgctca ggcgatggca gtgcttgaag ccatgaagcc gattagcgca
    22381 cgaagggagc atgttttccc aagtcttaaa aatccagtgc aaccaatgag cagccagaca
    22441 gcaaacgcag cattacggcg aatgggttac actggtgtgc tggtatctca cggactacgc
    22501 gccatattca gcacagcggc gaacgaagaa ggatttgagc cggacgtaat cgaggccgca
    22561 ctcgcacacg tggacacgaa cgaagttaga cgggcataca accgaagcaa ctacctggaa
    22621 aaacgtaaag tgttaatgtg ctggtggggt gattttgtgg aagcagccgc aaccggaaca
    22681 accatcgcca gcgggcacag aggattacgc ggaataaaat aaaatattaa aaaaattatt
    22741 agctcctaat tggtatcagt taggagccta tttggagcta atttaaattt catattttta
    22801 tgttagaaaa ttaattttaa atttctaacg ccattatcta aagttagaag ccccattatt
    22861 taaagttaga agaaaaataa ttattttttg ctaaatggct cttgacttat ttttttatga
    22921 ttttgtatgc ttattacatc acttaccgtt attttcattc aaaaagcgat attttgctgt
    22981 ccagaggaaa aataagatga gggctattct tttacgacca gaggtaaagg ctttgctacg
    23041 cattaaatct gattcgaccc taacgaagat ggttaacaaa ggcgggttcc ccaaaggatt
    23101 ccgtgtcggg ctgcgtcgca ttggctggta tgaagatgag gtactcgcct ggttgaaaga
    23161 acgtgagagg gaaagtcgtg aagcgtaagt gtgattaacg ttcgcctgat tactatcaag
    23221 taataagaca attaagagag aacattatga atactggcaa aaatgcccaa gcaggggcag
    23281 gttttcgttc aaccataaaa aatatggctg atgttattat tcagttcaaa ccttacaaaa
    23341 gaatcaattg aactaatcgc acttccgttt taaacggaaa cgtacaatcg catagcttaa
    23401 acgacaaaag aaatatgctt tttacaaata aaataaaggc gaccgcagag ggccgccaga
    23461 ggacatgtat taaacttgaa cgcatcgcca acaatgccat atttacagct ggtgggcaat
    23521 gtgatttgtg tggtgggcac accttcaaca catccgacca catgggccgg aaaacgggat
    23581 atactcgccg aatcccttat aaaacgggga tcgggattac caccccgctg aagcaaaagg
    23641 cgacacatga cgcgccagcg tcttttttgt tgtctgtaca cacgcacacc ttatcaatgg
    23701 tgggctgtat ggggctgact tcggtcaggc tggtctcctt ttgcgccagt tgtggtaacc
    23761 ctgtacagtc caccgccagc gagcttacca cctcctgcgg tgggtatatc ccaacgcaaa
    23821 aggaggctgc caaatggcta ctatccctgc cctcgcacat actcagaacg cctttatctg
    23881 gcgctttatc atcgttaatc cgagtgatta ccgcgtcgtt cacgtcgtgg cgtcaaccga
    23941 acgagaagcg cgtaactatt gcccgtccgg ttgtgttgcc gtattcgctg cccgtattcg
    24001 tcagggggta agtcatgctt aagcgattcc agataacagc acgcgctgta aatcatcaag
    24061 gccacgttat tgatattcgg caaaacgtga acgccaccag cgttaatgca gctatagcaa
    24121 tattgaaaga agagttaatc ggctgtgggt tcacgcaggt gatgatccgt gccgtagttg
    24181 aagtgtcgac tgttcattaa tctcattaat acagctttaa caaaaacgta attcgacaaa
    24241 tgaaagtaaa aaattctggc tttactgcca gcggccccgt tcggcctgaa atccacaccg
    24301 gcgatatttt cttcgataaa tacggctgga tggtaaaggt gataagcgtg gaggaacggc
    24361 gcatcacctg ccgtcgtgaa ggatataact acgactgtgt aacgcctgta tatcagttcc
    24421 ggcgtgattt ctccctgatg aaggccgcgc cccgcagtaa acctactagc agggaaaaag
    24481 agcggggaaa tatccagaaa atgaaaagca tgattaacac attcaggggc aagaaatgaa
    24541 acgtgcaccg aatttaaagc atcagccagc cgacaaaatg acggaggtta tcatttttgc
    24601 gggtagtgat gcctgggcac atgcgaagca gtggcaggag atggacgggc gacttgctgg
    24661 cgataacgtg cctcctgtct ggcttggaga tcagcaactg gcagaactgg aaaatctgaa
    24721 catcattgac gacgggcgct attgtgtccg gctgtacaag gcagggcaca tcaagccatc
    24781 aaacatcaat accattgcgc aaaagctggc ggcggcaggc atacgggatg cgaattatta
    24841 ccccgacggg atgcacagcc agaagcggga gaactggcgc gaatatctgg aacgtgagcg
    24901 ccaaaacatc gatgacgggc tggctatccg gcttccgaca aaagaaaacg ccaccggaag
    24961 tcatggcgat gatgaattaa agccccgcgt tgaaagccgc gcagatggtg ttttctgggt
    25021 aacacccaaa gtggacaagc agacaggcga gataatcaga cctgaaacgt ggttatgttc
    25081 cccgcttgaa ctactgggaa cggggacgat cggcagggag catcaccgcg tgatgcgctg
    25141 gaaaaaaacg gcaaatcatg aagtcatcac gatggcggtt ccgtgcggtg gcattggcga
    25201 ccgtgacggc tggcggttgt tgaaagacca cggccttaac gtgacaacca acggcaaata
    25261 cagagcaata ctggctgact ggatgcagtt aagcggaaac catgaggaat ggcagttaag
    25321 cacaacaacg gggtggcatt ttggcgcgta catcatgccg gacggttcag tcattggtga
    25381 gtctgaaaaa ccgatcctgt ttaccggaaa aacggctgcc gttaatggtt attccgtagc
    25441 aggaacgacg gagggctggc gcgatacagt ggcgcggttg gctggtggca atccgtccat
    25501 gatgctgggg gtggcggtat cgttatcagc gccattaatc gggctggtgg gcgctgacgg
    25561 tttcggtgtg catcttttcg aacaatcatc ggcaggtaag accaccacgc agaacatcgc
    25621 atccagtttg tggggagagc cggacgcaca acggctgacc tggtacggaa cggcgttagg
    25681 tatcgccaac gaggccgaag cacacaatga cgggctgtta cccctggatg aaataggcca
    25741 agccggaaac gcacgggagg tgtccacgtc ggcttatacg ctgtttaacg gttcgggaaa
    25801 attacagggg gcgaaggacg gcggcaacag ggagataaaa cactggcgaa cagtggcaat
    25861 cagtaccgga gaaatggacg ttgaaacatt cctcaaaagt gaggggataa aagtcaaagc
    25921 ggggcagctt gtccggttgc ttaacgtgcc gatggagaag tccacgcagt ttcacgaata
    25981 cagcacagga aaggcgcacg cagacgcgtt aaaggatgcc tggacagcaa atcacggggc
    26041 ggcaggtcgt gagtggatta aatggctagc agcccaccag cagaaggcta aggacacggt
    26101 aagggcatgc cgtgaacggt ggcgcaacct gataccggag agctacggcg aacaggttca
    26161 ccgtgtaggg gaacgattcg ccatactgga ggccgcgctt gtgctgtcag gcaatgttac
    26221 gggttgggat gttcaggcgt gccgtgatgc cattcagcat aactttaatg cctgggtgaa
    26281 ggagttcggc acgggtaaca aggaacacag gcagattatt gaacaggcag aggcgtttct
    26341 tgctgcctac ggcatgagca ggtttgcgcc tgtcaattat gaccctgcaa gcctgccgat
    26401 ttcagaactg tacggctaca gggaaagcga cgggcgttac ggtgagtctg ttttgtttta
    26461 cgtcctgccg gagccattta aatcgcatgt ggcgaaggga tttaacaagg atgccgtagc
    26521 aagagtcctt cacgaagctg gaatgctgaa aaagccagcc agcggggaag gatggcaaat
    26581 cagaacacca cgtcttaaac acatgaaggg ggcacgacta agggtgtacg gccttttact
    26641 tgcacaggat catgatgatg aaggaggtta atctctcgcg tgtaggttta aaaacacttg
    26701 tcccacctgt cccaacctgt gggaaacaca cataacacac tgaattatat tgggtaacat
    26761 cagaaaaagt gggacaagtg tgggacaagt taaggtgatt ttgggacaag ttaggagcat
    26821 tttgggacaa gtcagcaggc gttatttttt gcctggctgg tggacattaa aaatccaacc
    26881 aacatttaaa accggagatc agacaatatg acggcacaaa tatcagctta cgggcggctg
    26941 gtagctgacc cgcagttaaa gaccaccagc aagggaacac caatggcgct ggtgagtatg
    27001 gctgttccca ttccgtgcaa tcaggcagat gacggacagg cgacgatgtg gttatccgtc
    27061 ctggcatttg gcagacaggc cgacgcactg gcaaaacact gtaagtatcc cgcataatcg
    27121 tgccattcac atttagagat cctccggcat actcaatctg ccaattaagg agatcgctat
    27181 gcgtaaagcc cgttttactg agcatcagat catcgccgtg attaagtcgg ttgaagccgg
    27241 acgaactgtt aaagatgtct gccgggaggc cggtatctct gaagccacct actacaactg
    27301 gaagtccaga tacggtggca tggaggcttc tgatattaaa aagattaaag agcttgagga
    27361 cgagaaccgc cgtctcaaac agatgtttgc cgacctgagc ctggagaacc gggcgctgaa
    27421 ggacgttatc gaaaaaaagc tttaaaacca gcctgtaagc gtgagctggt cactcatctg
    27481 ataacgacat tcg
//
LOCUS       DADLDT020000055        26652 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000055 DADLDT020000000
VERSION     DADLDT020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26652
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.055"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(56..196)
                     /locus_tag="JQH22_002157"
     CDS             complement(56..196)
                     /locus_tag="JQH22_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFB2206470.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            438..731
                     /locus_tag="JQH22_002158"
     CDS             438..731
                     /locus_tag="JQH22_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFB2206471.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            803..1462
                     /locus_tag="JQH22_002159"
     CDS             803..1462
                     /locus_tag="JQH22_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFB2206472.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1554..2000
                     /locus_tag="JQH22_002160"
     CDS             1554..2000
                     /locus_tag="JQH22_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFB2206473.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2207..3107)
                     /gene="hlyE"
                     /locus_tag="JQH22_002161"
                     /pseudo
     CDS             complement(2207..3107)
                     /gene="hlyE"
                     /locus_tag="JQH22_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3480..3899
                     /locus_tag="JQH22_002162"
     CDS             3480..3899
                     /locus_tag="JQH22_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFB2206474.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3899..5167
                     /gene="umuC"
                     /locus_tag="JQH22_002163"
     CDS             3899..5167
                     /gene="umuC"
                     /locus_tag="JQH22_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFB2206475.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5213..5743)
                     /gene="dsbB"
                     /locus_tag="JQH22_002164"
     CDS             complement(5213..5743)
                     /gene="dsbB"
                     /locus_tag="JQH22_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFB2206476.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5889..7430)
                     /gene="nhaB"
                     /locus_tag="JQH22_002165"
     CDS             complement(5889..7430)
                     /gene="nhaB"
                     /locus_tag="JQH22_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFB2206477.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7651..8370
                     /gene="fadR"
                     /locus_tag="JQH22_002166"
     CDS             7651..8370
                     /gene="fadR"
                     /locus_tag="JQH22_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFB2206478.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8422..9954)
                     /locus_tag="JQH22_002167"
     CDS             complement(8422..9954)
                     /locus_tag="JQH22_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFB2206479.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10284..11582
                     /gene="dadA"
                     /locus_tag="JQH22_002168"
     CDS             10284..11582
                     /gene="dadA"
                     /locus_tag="JQH22_002168"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFB2206480.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11592..12662
                     /gene="dadX"
                     /locus_tag="JQH22_002169"
     CDS             11592..12662
                     /gene="dadX"
                     /locus_tag="JQH22_002169"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFB2206481.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12830..14244)
                     /locus_tag="JQH22_002170"
                     /pseudo
     CDS             complement(12830..14244)
                     /locus_tag="JQH22_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14855..16591)
                     /locus_tag="JQH22_002171"
     CDS             complement(14855..16591)
                     /locus_tag="JQH22_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFB2206482.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16686..17600)
                     /gene="ldcA"
                     /locus_tag="JQH22_002172"
     CDS             complement(16686..17600)
                     /gene="ldcA"
                     /locus_tag="JQH22_002172"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFB2206483.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17700..18311
                     /gene="emtA"
                     /locus_tag="JQH22_002173"
     CDS             17700..18311
                     /gene="emtA"
                     /locus_tag="JQH22_002173"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFB2206484.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18313..19047)
                     /locus_tag="JQH22_002174"
     CDS             complement(18313..19047)
                     /locus_tag="JQH22_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFB2206485.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19248..19502
                     /locus_tag="JQH22_002175"
     CDS             19248..19502
                     /locus_tag="JQH22_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFB2206486.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19552..21012)
                     /locus_tag="JQH22_002176"
     CDS             complement(19552..21012)
                     /locus_tag="JQH22_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFB2206487.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20949..21506)
                     /locus_tag="JQH22_002177"
     CDS             complement(20949..21506)
                     /locus_tag="JQH22_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HFB2206488.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21532..22386)
                     /locus_tag="JQH22_002178"
     CDS             complement(21532..22386)
                     /locus_tag="JQH22_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFB2206489.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22386..23195)
                     /locus_tag="JQH22_002179"
     CDS             complement(22386..23195)
                     /locus_tag="JQH22_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFB2206490.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23205..23963)
                     /locus_tag="JQH22_002180"
     CDS             complement(23205..23963)
                     /locus_tag="JQH22_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2206491.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23960..24940)
                     /locus_tag="JQH22_002181"
     CDS             complement(23960..24940)
                     /locus_tag="JQH22_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HFB2206492.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24940..25962)
                     /locus_tag="JQH22_002182"
     CDS             complement(24940..25962)
                     /locus_tag="JQH22_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFB2206493.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26311..>26652
                     /locus_tag="JQH22_002183"
     CDS             26311..>26652
                     /locus_tag="JQH22_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206494.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6704 a         6494 c         6876 g         6578 t
ORIGIN      
        1 ggcacagttg gtgtggcggc ctcagtccgg aacaatttga aaaccagaac ctcgcttagg
       61 cctgtgtcca tattacgtgg gtaggatcac tatggccttt gctgaactca acattgacat
      121 tcttacccgc agcaaagctc gtggaggtta gcagtgcaag cagcaataca gccttactga
      181 tgctcttgat tttcattggt agtctcctgt tcagaatgag tacgatgaac tgttataata
      241 taacaatccc ttacgggaga attccagtgg cttttctgcg ttcggtaaaa tcccggcgcg
      301 gcattttttg ccggggggat tacttataaa ggggcatgga ctcaacaaac cgcagaaatc
      361 tacagctggc tggcttgtgg cgcgggtttc attgatgtta gcttatgcca aaacctggca
      421 tcttaaagag taaaagtatg ttttgtgtga tttatcgaag cagcaagcgt gaccagacct
      481 atttatatgt cgaaaaaaaa gacgattttt cgcgtgttcc tgaggaactg atgaaaggtt
      541 ttggtcagcc tcagttagcg atgattctgc cgttggatgg gcggaagaaa ctggtcaatg
      601 ctgatattga aaaggttaaa ctggcattaa ccgagcaagg ttactatttg cagttaccgc
      661 caccacccga agatttgctg aagcaacatc tttccgtcat ggggcagaaa acagacgaca
      721 ctaacaaata accgatatcc ggcggtggca ttatctttgt cggcgcgggt tttcatatcc
      781 acgataaggt gaggggaacg ttatgtatca acatcacaac tggcaaggtg cgctgctgga
      841 ttatccggtg agtaaagtag tctgtgttgg cagtaactat gccaaacata ttaaagagat
      901 gggcagcgca gtgcccgaag agccagtgct gtttattaaa ccagaaacgg cactgtgcga
      961 tctgcggcag ccattggcga tcccatccga tttcggttca gttcatcatg aagtcgaact
     1021 ggcggtgttg attggcgcga cgctgcgtca ggcaacggaa gagcatgtcc gcaaagccat
     1081 tgccggttac ggcgtggcgc ttgatctgac attgcgtgat gttcagggaa aaatgaagaa
     1141 agccgggcag ccgtgggaaa aggctaaagc gtttgataac tcttgtccgc tttccgggtt
     1201 tatccccgcg gcggaattta ccggcgatcc acaaaataca acgctgggcc tgagcgtaaa
     1261 cggcgaaaaa cgccagcaag gtacgactgc ggacatgatc cataaaatcg ttccgttgat
     1321 cgcttatatg agcaagtttt ttaccctcaa ggccggtgac gttgtgttga caggcacgcc
     1381 tgatggcgtc ggcccgttgc aaagcggtga tgagctgaca gtcactttcg atgggcattc
     1441 tttgacaact cgcgttttgt aatacttttt gccgccagaa agcggcggca aaacttgcat
     1501 cgctgtgcca gactggttat aaggtgcgtt ttaacgtaat ggcggaacac ctgatgagcg
     1561 atgtaccttt ctggcaaagt aaaaccctgg acgaaatgag cgatgcggaa tgggagtcgt
     1621 tgtgtgatgg ttgcggtcag tgttgcttgc ataaactgat ggatgaagac accgacgaaa
     1681 tctacttcac taacgtcgcc tgtcgccagc tcaatattaa aacctgtcaa tgtcggaact
     1741 acgaacgtcg tttcgagttt gaacccgact gcattaaatt aacccgtgaa aatctgccaa
     1801 cattcgaatg gctgccaatg acctgcgctt atcggttgct ggcggaaggt aaagatttac
     1861 ctgcgtggca tccgctactt actggttcga aagcggcaat gcatggtgaa cgtatctctg
     1921 tgcgtcatat cgcagtgaaa gaatcagaag tgattgactg gcaggatcat atcttaaata
     1981 aacctgactg ggcgcagtga taaatttaat agatgctgat gggggctttc ttaatatacg
     2041 tctggatgct cgccatttcg tagccctgtt tttgaaagcg ctctgtcata atgagagtta
     2101 cccgatacct tattatcaac aggtaactct caatctttaa aaaacgtctg tgtttttaaa
     2161 tgtgataaac cttatacctt gagtacatgg agagaataat cgcttatcag acttcaggta
     2221 cttcaaagag tgtcttttta ccgtgtcttt tctgatactc attacaggtg ttaatcattt
     2281 ttttggccgc tgcttttagc aaagaaagca ttaaatcatc ataatcaaca tagaatctgg
     2341 ttgtttcagt ttccgttttt atctccccga tggcggctat ttcggtggtt aatttcaatt
     2401 tggcggcatc gatatcttta ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac
     2461 tctgcacaga ttttaacttg ttcttcaatt ctggaatcag tttcccttca actacgcccg
     2521 cagcaataga ataggaaatg attaaaccaa atggaccggc gacgacatcg gctgcggcac
     2581 cggcatacgc ttccttcctg attttatcta cctgtgactg gaaatagctg cttttttctg
     2641 aaaaatcatt ggttaactgg ctatctaacg ccagcagttt cccggaagcg ttgttgaaac
     2701 tttgtgagct taccagcagg gatttttgcg cttcattcag cttcgtgatg ccgtcatcca
     2761 gtaccttaat gaggggcgga tgctttcttc tcattgtact catcaaatag caaaatatac
     2821 gctgcgagca attgcgtcgc aacaccacac cattcataca ctgtttgggt tgcttcaaaa
     2881 tacttatcct ggctatccat aagtaaggtt ttaatatcgc cgactaaaac ggaggctgcc
     2941 tgtgaatact cctgtttaaa gcgacttaac tcttttatgg tttcatcaaa ggtctgccag
     3001 gggatgacct gatcgagata tttattataa agatctaatg ctccatctgc ggtttcgatt
     3061 gcgtttttaa ctacttctac cgttttatct gcaacgattt cagtcataat cattcgcctc
     3121 tttaaatata taaattgtaa tgaaactcct gttttacaac tattaataaa ttttacttca
     3181 tctaattcat agttagccgg gcgggatgcg tcaatgtctt tatttctatt aatatgataa
     3241 atatcaaaca atgtttaatg tcattatggc gaatgcttct attctatttt tttgccgggt
     3301 gatatttttc atttctgctg gatgagcgtc gtcgccagaa ggccacgtga gcacaggata
     3361 agagaacgaa aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg
     3421 atctgctggc aagaacagac cactgtatat aaaaacagta taacttcagg cagattatta
     3481 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc
     3541 ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctga
     3601 atcaactgtt gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta
     3661 tgattgatgg tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca
     3721 gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac
     3781 tacgcccgac ggtacagctt attcccatga acagtgcgta ctcgcccatt accatcagta
     3841 gcgaagatac gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat
     3901 gtttgccctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga
     3961 tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa
     4021 cgctgaggca aaggcgctgg gcgttaaaat gggcgatccc tggttcaaac aaaaagatct
     4081 gtttcgtcgc tgtggtgtgg tttgctttag cagcaattat gagctttacg cagacatgag
     4141 caatcgggtg atgtcgacgc tggaagaact atcgccccgc gtcgagattt acagtattga
     4201 tgaagcattc tgcgatctga caggtgtgcg taattgtcgc gatctgactg attttggcag
     4261 agaaattcgc gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca
     4321 gaccaaaacg ttggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg
     4381 tggggtggtg gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt
     4441 ggatgacgtc tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa
     4501 aaccgttctc gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcat
     4561 gctcgaaaga acggtgcgtg aactgcgcgg cgaaccctgt ttgcaactgg aagagtttgc
     4621 accgacgaag caggaaatta tctgttcccg ctcgtttggt gaacgcatca cggattatac
     4681 gtcgatgcgg caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagtga
     4741 gcatcaatat tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga
     4801 accttattac ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga
     4861 tatcattaac gctgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca
     4921 aaaagcgggc gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt
     4981 cgatgacaac gcaccgcgcc ccgggagtga gcaattgatg gcggtaatgg atactctgaa
     5041 tgctaaagag ggcagaggaa cactctattt tgccgggcag gggatccaac aacaatggca
     5101 gatgaagcga gctatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt
     5161 caaataaata tagcggcagg aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac
     5221 cgaacagatc acgttttttc gctttaaacg gctgggaaat caccaccagc accgcgacaa
     5281 tcaggtaagc gataaaaata ccgagcagcc actgcggcat ttccatacct aaaaaatccc
     5341 actgacgctc ggcgcaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg
     5401 gcagccattc cgggaaacga accataaaat cacaggtggc aaatggcgaa ggatagagct
     5461 gaagcatggt gtgctcgtaa gttaactgca caccgcggaa cgcactatac aaccagataa
     5521 ccatcgctac ataacgcagc ggagttttcg gggcgatcgc gccaatcagc gcagcaccca
     5581 gaacgccgaa taacgcgcag cgttcataaa tacagagcac gcaaggtttc agtaacatca
     5641 catgctggaa ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc
     5701 acgcgccccg gccttgtgaa cattggttca aaaatcgcaa cataatcatt tccctgcaat
     5761 atgcatagag tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa
     5821 taggacgcaa taggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta
     5881 agttgttatc aaagcgttgc tatccagccc atttgcataa accattcggt tacaggggca
     5941 agcgtaaact cgacgcagag caagccgacg agtgtcagta cgagggtgta aggcagggcc
     6001 atccacacca tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg
     6061 aacaggaacg cagcctgacc gttcggcgta gcgacagagg gcagattggt accggtatta
     6121 atggcgaccg ccagcagctc gtattgcttc aacgtgatag cgccactttc cattgccgct
     6181 ttcgcttcgt tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga
     6241 ccgttgaaaa tatagaacag cgacagctga gcatgttcag atgcctgcaa cacaaactga
     6301 ataatcggcg aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc
     6361 aacagtgcgg tgaatggcag agattcggtg aaggctttac cgatagaatg ttcatcggtg
     6421 acaccggtca atgatgttgc cagaataatg actgacaaac caatcaagcc aacttccgcc
     6481 agatgcaacg ccagcgcagt caccagccag acgccaataa tcgcctggac aatcagacgg
     6541 attttatcct gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg
     6601 cggactttct ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc taccagcagg
     6661 caggttaaca ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa
     6721 tcgccaaaat gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg
     6781 gtcattacgc cgcctaatgc ggtgccgaca ccggcatgca tcatcaggct acgcagaaag
     6841 ccacggaact gttccagaac cacgttgtaa tgcttgtcga tatgactatc gtcttgcagg
     6901 tcggtgtctt cggtacggga agaggctacg cgatgataaa taccataaaa accgactgca
     6961 acgctgatca ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgctgcc
     7021 gccacgcaaa aagagagcga cagcagcatt ttggagcgaa tgcttaacag caaacgggta
     7081 aatatgaaca gcaacagctg tttcataaaa tagatacccg ccaccataaa catcaataac
     7141 agcaagactt caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg
     7201 atgaataccg cttcgatagc caacagacca ccggggagta gcgggtagca tttcagggcc
     7261 atcgccagag tgaaaataaa ttccgctacc agcaaccagc ccgcgacgaa agggctgatg
     7321 aggaaaatta acgggtttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg
     7381 gactggccca aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc
     7441 cttaccttac aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc
     7501 aggagtgagg caagtcttga tagtcaaggg gaaagagatg cggaaaatga agccttgatc
     7561 cctttttctt ctttttgtct gctatcagcg tagttagccc tctggtatga tgagtccaac
     7621 tttgttttgc tgtgttatgg aaatctcact atggtcatta aggcgcaaag cccggcgggt
     7681 ttcgcggaag agtacattat tgaaagtatc tggaataacc gcttccctcc cgggactatt
     7741 ttgcccgcag aacgtgaact ttcagaatta attggcgtaa cgcgtactac gttacgtgaa
     7801 gtgttacagc gtctggcacg agatggctgg ttgaccattc aacatggcaa gccgacgaag
     7861 gtgaataatt tctgggaaac ttccggttta aatatccttg aaacactggc gcgactggat
     7921 cacgaaagtg tgccgcagct tattgataat ttgctgtcgg tgcgtaccaa tatttccact
     7981 atttttattc gcaccgcgtt tcgtcagcat cccgataaag cgcaggaagt gctggctacc
     8041 gctaatgaag tggccgatca cgccgatgcc tttgccgagc tggattacaa catattccgc
     8101 ggcctggcgt ttgcttccgg caacccgatt tacggtctga tccttaacgg gatgaaaggg
     8161 ctgtatacgc gcattggtcg tcactatttc gccaatccgg aagcgcgcag tctggcgctg
     8221 ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg cgcacgatca ggtgtacgaa
     8281 acagtgcgtc gctatgggca tgagagtggc gagatttggc accggatgca gaaaaatctg
     8341 ccgggtgatt tagccattca ggggcgataa tcccttccgt ttaaagagca accccctcaa
     8401 acgaggggtt ttttgttgtt tttacagatt tcccattctt ggcgggcaac gttccagcaa
     8461 ctcgatgctg ccgtcttcgt tttgctgttc gagcatcaca tcaaatcccc acaggcgatg
     8521 cacatgcttc aggacttctt tgcgcccccg atccagcggt gcgcgattat gtggaatata
     8581 acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc cagatctgaa tattcggctc
     8641 cagattactt aagttatatt gcgacgataa ccggttacgg atctcccgat aaccttcttc
     8701 attatgaata gcggaaatct ccagataatt atgccgatca tcatccagca cggtgaagaa
     8761 gcggaaatca cgcatcactt tcggtgacag aaactggctg ataaaactct catctttgaa
     8821 atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa ccggcgatat ccgggaacca
     8881 gtatttgtct tcttccgttg gcgactgaca aatccgttta atatcctgga acatggcgaa
     8941 cccgagggca tacgggttga tgccgctgta ccacgggctg ttataggggg gctggaagac
     9001 cacattggtg tggctgtgca aaaactccag cataaaacgt tccgttactt tcccttcatc
     9061 atacagatgg ttaaggatgg tatagtgcca gaaggtcgca cagccttcgt tcatcacctg
     9121 agtctgtttt tgcggataaa aatactggct caccttacgc acaatacgca ggatttcacg
     9181 ctgccatgat tccagcagtg gcgcattttt ctccataaaa tagagcaggt tttcttgtgg
     9241 ttcggacgga tagcggcgcg cttcagcaac cgttttctct tcctcgcgct tcggcagggt
     9301 acgccagagc atattgacct gactttgtag atactcttcg cgacttttct gccgggcttt
     9361 ctcttcttgc agcgagattt tttgcgggcg tttgtaccgg tccacgccgt agttcatcag
     9421 cgcgtggcac gagtccagaa gccgttctac ttcatcaacg ccataacgtt cttcgcactc
     9481 ggtaatatat ttacgggcaa aaatcagata atcgacaatc gaactggcgt cggtccaact
     9541 gcggaataag caattgtttt taaagaaaga gttatgtcca tagcaggcgt gtgccatcac
     9601 cagcgcctgc atagtaatgg tgttctcttc catcaagtaa gcgatacacg ggttagagtt
     9661 aatgacgatt tcataggcca gcccttgctg accgtgctta tacagccgtt cagtctcgat
     9721 aaacttttta ccgaatgacc agtgcggata gttaattggc atgccgacgc tggaataggc
     9781 atccatcatc tgttctgagg ttatcacttc aatctggtgc gggtaggtat ccagccggta
     9841 gagtttcgcc acccggtcta tctctgccag ataaacatcc agcaggtcga acgtccagtc
     9901 gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa tcgatcgtcg ccatacacgc
     9961 acctcattgt tgtcggcgct ctctgtgtgg agcacctcat ttcaagcata gaacacctgt
    10021 taaaaaccgc gtcgccggag aatttttttc tttgcgattt cttattatca gagtgccact
    10081 aatccgcttc tgaacggaat tttatgctgg ataaaaaggg cgttcagcag gagatactaa
    10141 agacgccata ttgccgcaga gtcagggaga tgtgagtcag ctcaccataa aaaagccgta
    10201 tgttgaataa tattttcaac tgagttatca agatgtaatt agattattat tcttttactg
    10261 tatctaccgt tatcggagtg gctatgcgag ttgtcatact gggaagtggt gtggtaggcg
    10321 ttgccagcgc ctggtactta aatcaggcag gacatgaggt caccgtcatt gatcgggagc
    10381 cgggggccgc cctggaaacc agtgccgcga atgccgggca aatctccccc ggatatgctg
    10441 caccgtgggc agcaccaggt gtgcctttaa aagcgattaa atggatgttc cagcgccatg
    10501 cgccgctggc ggttcgtctc gacggtacgc agtttcagtt gaaatggatg tggcaaatgt
    10561 tacgtaactg cgacaccagc cactacatgg aaaacaaagg gcggatggtg cgtctggcgg
    10621 aatacagccg tgattgcctg aaagcattgc gcgccgaaac caatattcag tatgaagggc
    10681 gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg
    10741 atatcgccgt gctggaagat gccggcgtac cgtatcagct gctggaatcc agccgcctgg
    10801 cggaagtgga tcccgcgctg gcagaagtag cgcacaaact gacgggcggc ctgcagttac
    10861 ccaatgatga aaccggagac tgccagctgt ttacccagaa tctggcgcgg atggcagagc
    10921 aggcgggggt taaattccgc tttaatacgc ccgttgacca actgctttgc gacggcgagc
    10981 aaatctacgg cgtgaagttt ggcgatgaag tgattaaggc cgatgcgtat gtgatggcgt
    11041 ttggttctta ctcgacggcg atgctcaaag gcattgttga tattccggtg tatccgctga
    11101 aaggctactc gctgactatt ccaattgcgc aggaagatgg tgcgccagta tccaccattc
    11161 ttgatgaaac ctacaaaatc gccattaccc gtttcgataa ccgcattcgt gttggcggaa
    11221 tggcggaaat tgttggtttt aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaaa
    11281 tggtggttcg cgatctctat ccacgcggcg gtcatgtcga gcaggcgact ttctggacgg
    11341 gtttgcgccc gatgacgcca gacggtacgc cggttgtcgg gagtacacgc tttaaaaatc
    11401 tgtggctgaa taccggtcac ggcacgctcg gctggacgat ggcttgcggt tccggtcagt
    11461 tgttaagcga tctgctctct ggtcgcacgc cagcgatccc atatgaggat ctaagcgtag
    11521 cgcgctacag ccgtggattt acgccatcac gtccgggcca tttacatggc gcacacagct
    11581 aaggaaacga gatgacccgt ccgatacagg ccagcctcga tctgcaggca ttaaaacaga
    11641 atctgtccat tgtccgccag gccgcgccgc acgcgcgcgt ctggtcggtg gtaaaagcga
    11701 acgcttacgg gcatggtatt gagcgtatct ggagcgcact cggggccacc gatggctttg
    11761 cattacttaa cctggaagag gcaataacgt tacgtgagcg cggctggaag gggccgatcc
    11821 tgatgctgga aggatttttc catgctcagg atctggagat ttatgaccag caccgcctga
    11881 ccacctgcgt acacagcaac tggcagctca aagcactgca aaatgcgcgg ctaaaagcac
    11941 cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg gttaggcttc cagtccgatc
    12001 gcgtgcttac cgtctggcag cagttgcggg caatggcgaa tgttggcgaa atgaccctga
    12061 tgtcgcattt tgctgaagcg gaacatcctg atggaatttc cggcgcgatg gcgcgtattg
    12121 agcaggcggc ggaggggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt
    12181 ggcacccgga agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc
    12241 cgtccggtca gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca
    12301 gtgagattat tggtgtccag acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct
    12361 atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc aggatacgcc gacggttatc
    12421 cgcgccatgc gcctaccggt actcctgttt tagtggacgg cgtgcgcacc atgacggtgg
    12481 ggaccgtctc gatggatatg ctggcggttg atttaacgcc ttgcccgcag gcggggattg
    12541 gtacgccggt tgagctgtgg ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg
    12601 gaacggtggg ctatgagttg atgtgcgcgc tggcgttacg cgtcccggtt gtgacagtgt
    12661 aacttgttgt aagccggatc ggaggcagcg tcttctggtt gcgaaaaaat catccatccg
    12721 gctggtcagc aactgtagtt gttaatgtga cagagccatt gcccatgata gtgtccatta
    12781 aaagaatgga cactatttcc ccggaacctg aactcgccgc acaggcgttc tacataaaac
    12841 gcttacgctt cattgttgac tcggagtcga cttcagataa atcacgcttc acccttgatg
    12901 cagattcggg ttcatgttca gattgttgat taacaggctg cgagtcctgt tgtctttcca
    12961 gactcaacag gcccaactgt tgatccagga gctccaggtc ctcctgtacc ttcacgcgct
    13021 ccaggagttc cgggtcatcc aggaactcca gatccataag gtcctccagc tcctcgagtc
    13081 cttccaggtc aacctagcct tccttgtgca aaaagtccaa aaggtccttc aggccctgct
    13141 gttttttcag gccctcgacg gctttgagca gcgtcggtaa attgtctttg tcccacgggt
    13201 taccattaca gtcaaacacc ttcaggctct ggggctgcgg cacctgtacc gttttaaact
    13261 tgttttctcc acagtagtaa attattagtt tgggcggcag ccgtggtgaa gagatcaaat
    13321 cgttactaga acagtcaaat actttcaggc gagtcaagtt ctccagcatc tcatcaggta
    13381 ttgagttaat attgttgccg gcacaggata gtttatacag gtactggggc atcgacggcg
    13441 gtaaaaattg caagaagttt tcagaacagt taatcactct caggttagtc gacagctcag
    13501 gcagccagat aatactgttt tcatagcact tcagtaccat cagttttgag ggctgcacct
    13561 tcggtaacaa gatcaagacg ttatctgtac agtcaagcac cagcagccac tttggcatca
    13621 ccttcggtaa cgcggtcaac acgtttttac tgcagtaaaa ttcaatcaga tgtggaggca
    13681 aattctcagg gagtgaagtc aatcccaggt tgctaagatt gagcatactc cgtcttgtaa
    13741 tgagacattc cttcattctg cgcaatgctt ctgcccggtt ttcaatccgg cttgctggca
    13801 ggttttcttc caggatattt tccttgattt cttccagact ttctttcagg tcttcttggg
    13861 gaatttctga ctgtttgttt tgctggtttt cttccaggat ttcgtacagg tttttttgca
    13921 ggttttcttg caaaccatcc tgctgtatag cccattcgca ccatttggcg accatcatga
    13981 actcgttatg ttgctgattc agacgagtcc ccaacgtgat gatggtattg ttgtccacct
    14041 gtatgttcac tttgcccgcg atactgaggt caatgatctg ccagggggca tcaccccagc
    14101 tgaatttttg cgggctgaga tacttcatgt tctgcaaaat ttgattcttg agaagagccg
    14161 ccctggcggg cgtcgtacgc ctgggttaac ggaagatggg ggaatggcgg gggcgaatgg
    14221 gcagatgtct ttaaagctag ccataatgct acgtttcctt gaaataaata tcctctggca
    14281 gcgctggaga cgtgaatgtg gtcaccgcaa aggagccaca ggacgtcatt tgcgctgata
    14341 tcactactca tcgttgactt tttccctggt cagaaatgcc gtgtcgggtc tggtacatgc
    14401 tgtattacgg cctaggtagc tctgacgtat taaagaaaat acgagagtga acgacccgga
    14461 aataatcgtt attaagcaaa ggaataacta taagaaatcg ctcatgcatc cggtgctaca
    14521 ggcggatgaa acgcctattg tgtgggctat gtcagtgcgg caggttgcgt gggggaatat
    14581 gttcaggcga tgtcaggcag gagcccctga tggttgaccg gtaatgcggg gctggttttt
    14641 attacctgtt acagtcggaa aaagccctta cgctgacttt cttgagccgg tgttttgagt
    14701 tcattagcca gagcctgcaa tggtttgtca tccataattt aaccttcttc tgatgttggg
    14761 atgagcatat caaaatcagg aaatgacaag tttattacag gctcaggctg cgttcagtaa
    14821 agcatcgcat ccggcgatag atgctgcatt aaatttaaga ttcagcttcc tcttcagcca
    14881 cccgaacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
    14941 cggcaaactc tacctggtca ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt
    15001 cacccagcgt ttgctgttta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    15061 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    15121 gcgcgaccgg tggcgactgg ctgaacagtt taccgagcgc cgggagatcg cgttcccgac
    15181 caattacaca caatacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    15241 ggttatcacg aaacagggct gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    15301 ccgcacctac acaccattta tcagcactca attgataaac aaactgctcc cacggatttt
    15361 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc gacgggcgga accaccactt
    15421 tggctttttt cgccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    15481 caaagaaggc gacattaaag aacagtcgcg cattctccag ccccgccatc atcgggaaca
    15541 cggctaggat gattggcacc gcgccgcgta atcccaccca gctgataaac acgcgctcac
    15601 gcagattgaa accgcggaaa gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    15661 atatcatcca tgccgacaaa atgagcgccg gaatggcaat aggcagcaga tcgcttgggt
    15721 taaccaatag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    15781 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    15841 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    15901 cgccgcttaa tgccagcaat ggatataatc cggcaggcag ggcgatgcga ttaatcattt
    15961 gcagcagtaa ataaccaccg ccaagaccga tgacaatccc gaggccaaat tgctgcagaa
    16021 tatcgaccac gaacatccaa ctgacactgc tttcatgctg ctggatcatt gcaatcaggg
    16081 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    16141 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg
    16201 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    16261 ccatcatgcc ggttaaacca gaggtgataa gtacgcccag cgtcgccagc gacagcgccg
    16321 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    16381 tcgccagcgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    16441 cgacgccatc gactcccgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    16501 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    16561 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    16621 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    16681 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagcgcc ttcccgggtg
    16741 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    16801 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgta cactgactcc
    16861 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    16921 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    16981 aaagggtgca cgttaatatc ttccagcacc agaataccgt tctcaatttt tggcatccac
    17041 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    17101 tgacatgtcg ggccttcccc ttgccattca atggtgaagg tttcattgcg taacgccagc
    17161 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcac caaagtttgc taccagcatg
    17221 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    17281 tcgctatgtc cgcaaatgag taacgggtca tgttgttggc gagccaccag tgcctgccag
    17341 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    17401 ttgggggtag tcagtctggc aagggaattg agatcttcca gacgctccgt ttccgtaccc
    17461 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg
    17521 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca
    17581 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    17641 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    17701 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    17761 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    17821 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    17881 tcgctatcga atcgggtggt aatccgaacg cggtgagtaa atcgaatgcc attggtttga
    17941 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    18001 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    18061 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    18121 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    18181 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    18241 cgcgaaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    18301 acgcgatgta aatcagtcgc gcactttgtc cgctttttcc cgggcttctc gctcgagaga
    18361 gaaaataatc cgctgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    18421 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctggtgga
    18481 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatttgagc
    18541 gaagcgcata ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    18601 gcctaacgac aaatcataca agcggaaacg taacgtactg ttatccgcca gtttggtctg
    18661 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    18721 ccataaggtg ggaggcggta cggtaataaa tgccggaagc tgcaagtatt cactctgctg
    18781 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatgtg
    18841 ctgtgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    18901 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    18961 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    19021 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    19081 gtttatcggt catagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    19141 tcttaaatca gccgacaaac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    19201 gacgtaagca tttcttgcga ccgattcatc gaaacgaggg cctgaacatg ggaattattg
    19261 cctggattat ttttggcctg atagccggca ttatcggcaa gctaatcatg ccggggcgtg
    19321 atggtggtgg atttttcctg acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    19381 ggctggcgac catgtttggc attgacggct ccatcagcgg ttttaatttg cacagcttcc
    19441 tggtggcggt ggtgggcgct atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat
    19501 aagattttca taaggcggat agcgatacag atgccgctat ccgctttcac atcagaacgt
    19561 atactcgaca cctgcccaga aattgcgacc ttcttcaata aacccttcac tgtaggcata
    19621 ttgggtatca aacaggttat tgaccgacgc attgacgctg aagccatgac ctaaggtgta
    19681 atcggctcga atgtgggtca ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct
    19741 gtagctggag gaacgcgcct cttccgacag cgttacgctt aacggctccc atggtttgag
    19801 tgtcatccat gcggtcattg tctgcgttgg aagatcggtt atcttgccga tgtctttgcg
    19861 tttggcgtca gcgtggatca gagcgtagct caatcctaca tccagtatat tgctgatttt
    19921 ccccttaata ccggcatcca gaccgctgta atccaccgtg ccgctgttct gattttgaat
    19981 ggtatcggca ttgatattgt gcgagaggat ggcatcactc acccggttat agtaaacgct
    20041 gacctcaaat ccccagtcgc gcgtgaaggc accattccag gttaaatcca ccccgcgagc
    20101 gcgttccggc ttgagctgcg ggttaacaat cgctatctgg ttatacgcag gtttggatgt
    20161 ggtatagcgt tctttcagcg tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt
    20221 gtcttcattg gcaaagtggt atttccccat cacctgccag ttaaaagctg actgattgtt
    20281 gtcgtcataa tgggtgatgc tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg
    20341 ccagtcatag ctgattcccg ccacgacatc gacattatca gcagcagccc attgatattc
    20401 actggcgagc gaccaggtac gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt
    20461 ttcccggtgt acgtcatctt tccagttaac ggcaaacgac agcagatcgt tttcacgcac
    20521 atcggctgcc agttgtaatc cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc
    20581 ttttttattt ttcaaatcag ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc
    20641 gcgatacagc cgacttttca gggtaaaacg gtcgtttagt tgggtcgctc cctgataata
    20701 aaaactttct ttgtcatact ctggccactg ccagtagcgt gatttttgac cacaatttcc
    20761 gctgtatggc gggttatctt tttcaccatc ctgcttaatg taagtcagcg tgtattcatc
    20821 gttttcgcgc ggtgtaaaac cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt
    20881 aatcatcttg ccgtgtttgc ctgcaatatc attatttaca ccatgcggca ggccgagaaa
    20941 atcctgcttt agctggctac cgctgacttg caaataccca agatcgctgc tggcggcaaa
    21001 tgaagcatgc atatcgtagg cattgtcctg actacggctc catccctggc gatatcccag
    21061 acttgcttct tcagggtggt gatattaatg gctccgccca tctgattagg cccatgaagc
    21121 agcgacgaat accctttgga aacttcgact gcccccagat tgttggtcag aatccgcgcc
    21181 agatcgaggt tgccgtcata ggggacataa atgggcacac cgtcgaaata gaccggcacc
    21241 tgccgactat caaagccacg aacttttacc tgttcttcgt tgcggctgcc tgacttttgc
    21301 agcaccacgc cggggacgac acttaatgcc tgagcgacat tctgtttatc caaggccttc
    21361 atggtgggtt gatcgagaac ggtggtcgtc gtcgatgata ccggactgga ccatacggtc
    21421 agcgtgtcgc tctcctgagc cgctacggcc tgctgggtaa aggcaagtga taatgctgtg
    21481 cagagataac gttttttgag tctcatagaa taaattccgt ttaacaaaga attaaataga
    21541 ggctgtgggt tgcagactga ctttaatgtc agctggtgcc gcgtaataag gcgcggaggt
    21601 aataaaaagg cgaatgccgc agtcgaggta atttttgagt gtcgtcagat taattccgcc
    21661 ggtcagagcc agcgtgcagt gaggagccag cgacggcgct atttgcgcaa tttcagttgc
    21721 ctgttgcgga ctaaatttgt cgagctgaag tacgtctggt tgtgcgcgta acgcggcgat
    21781 agcctctttc ggcgtgtcgg cttcaacaac aattttcttc tcgggtgcgt ggcgacgtaa
    21841 ctgagcgatt gcgcctgacc agccctgatt gtcatgaaga aaatggcggt ggttggcaaa
    21901 caataatatg gtttccgcac atccggcacg atgaattagt cctccggcag ccagaattgc
    21961 ctgcgaagcc agtaagcggg tgcccggaat tgcttttcgg gtgcaggcga tattaccgtc
    22021 aggataacgt tcacgaagca atgccagcat ttgagcgaga taatcagaaa caccgcaact
    22081 ccactccagc acattctgga ctgccttcca tccctgatga agtgctgcgg cattaccctg
    22141 cgcacggatt agccgttgac ccgcgttcgc ttgtgaaccg tcgctgaccg cgtcatcaat
    22201 agttaatccc agcgtagtta acatcttaca cgcgacagaa acgccgctga cgcagccgcc
    22261 ctgacggtga aaaaacgcta tatagccagg ctggtgtcca atatttaatg ctcgcgtggt
    22321 caggtcacct ccctggatat cttccagcag taacgcatcg atttgtgcct gagaaaggaa
    22381 aatcatcaca gtgcctcctg cggtacgcta tcccaccaga tgagcgccca gtcgcggctg
    22441 gcaggggcga gggcacgagc atcttgttgt tgataccagt gatataaccg ctcacgctca
    22501 tcgtcgttaa gcgcgccaag actccagctg acgttctgct caaaacgttc ccaggtgctg
    22561 ttatcctgtt ggcaattttg tccacggata aaatcaacgt gggcataaat tcccatctga
    22621 tacaggacgt tgagcgcaaa gatatagttg ggtaattcga tcacttcccg accagccgcc
    22681 cgctgaatgg ctggagagac gaacgaggtg ctgaccagat gagttgtata gacgcgaagc
    22741 cgtgcctgat tattcagttt gctcattgca tggcgcatat cagccaccag agtggagcgg
    22801 gaagccacgg caatatcgca gcgtggcagg tcactccagt cctcttccca ggcgcgctgg
    22861 atccagtgga cattgtcagc cttcagtgcg gcggcgcggc gggcggcaac gttcagcatc
    22921 ccctgactgt aatcaacgcc ataaaccgta gtgagtttgt cagccagcgc cagcgatacg
    22981 gtgcctggtc cgcagcccat atcgaataag gtctgagcgc cttgcaggtc aattttagcg
    23041 atcagttgtt gcaggtagct gtccgttggc gaggcgcaat tttccgccat tttctccgcc
    23101 cgttgatccc agtgatcggg cgttttttct gtgcggtgag ccagtttgag ttgctgcaga
    23161 taaaggtctg caaaatcaat atcatcaatg agcatgattg ttccttagcg gctaattgca
    23221 gagaggtgat gatgaatttg gtcggcagaa acccgataca gggccgccag tttgttcgtt
    23281 gttaactgtt cggtcggtaa gccttgtgtt actcgtccat ctggctctac ttggataatg
    23341 ctgtcggcaa tggcgttggc atggagcgga tggtgagtcg acattaacat tgtcatgccg
    23401 ttatttttga gctgcgccag cgtgtccaga agttggatct gatgaccgaa atcgaggctg
    23461 gaggcgggct catccagtaa caacaagcgc ggttgttgga cgagtgcacg ggcgattaaa
    23521 gccagctgac gttcaccgcc gctaagcgta ttccagcgtc gtgtcgccag atgcaaaatg
    23581 cccagctttt ccagttgctc cgtggctttc aggcgttctt gcttaccggg aacggaaaaa
    23641 gcgcctatgg tgggggccag gcccatcaac accatatcca gcacactgaa agcaaaaata
    23701 ccgtcgtgcg cctgcggcac ccaggcaata gcctgtgcgc gctggcgatg gctgagtgtc
    23761 tgcacagaga ggctatccag taggatctcg ccttttaata acggtataac gccaagaatg
    23821 gagcgcatga gtgtggtttt gccgcaacca ttcgcgccaa gcagacaaca aattgttcct
    23881 tgcgacaggg tgaaggacac gtcgcgtagc actggatggc gggagtaacc cagagacgca
    23941 tgacgaacgg caagcaaagt cactggcgac ctccgcgtaa caacaaaagc aggaaaaacg
    24001 gtgcgccaac aaaagcggtc agaatgccaa gtgggatttc tgttgttcca atgctgcgcg
    24061 ccagcgtatc ggtaagcagg agcagaatag caccagtaca cattgccata ggcaacagct
    24121 gctgatggtt atgaccggtc aacaagcggg caatgtgcgg aacgaccaga ccgatccaac
    24181 caattatgcc cgctattgct accgtgctgg cggtgattag tgtggcgcag acaatcaatc
    24241 caaagcgcag acgcgtcacg ttcagtccca gcgaacgggc ttcgtcgtcg gaaagcgtga
    24301 gtaaattcat gcgccagcgg agaaaaaaca ggggcaagga gcctatgaga atgatcggtg
    24361 cagcatagca gagatcgcgt aaggtcacgg tggacagtcc ccccagcaac cagaaggtaa
    24421 ttgacggtaa ctgggtgtaa ggatcggcca gcgttttaat tagcgatatc cccgctccgc
    24481 acaacgttcc taacgcaatg cctaccagca ccagggttaa gatcggatcg tggcgggtca
    24541 cccgacgcgt aatcaaccag acgcccgcga ccaccatcag cccgccgcaa aatgcgtaca
    24601 gctggatgta aacaatgggt aatccccaca ggattgccgc gcaggcacca agtccggcac
    24661 cggcggagac gcctaaaata tccggtgaaa ctaagggatt acgcaacatt ccctgatagg
    24721 tggttccggc tcctgccagc gctgcgccca gtaacagcgc agcaagaata cgcggcacgc
    24781 ggatttgcca gaagacaatt tgttcctgca cagactcatt ttcttgacga agaattagcg
    24841 ccagcagttg ctgcatatcg agatgataag ccccacttac ggcggcaaaa gtgatactga
    24901 tagctgccag cagcaaaata catccgttta cgattctcat tagctcgcca ccaatttttg
    24961 atagtcggca tcactgagtg aacaatgcca gaacagttgg gcgtaatgct gcatgtcact
    25021 tttaaactgg cgattgatgg cgggatccag ccaggcgtga agtctgcgta atcccagaag
    25081 acggttgatt cccggcgggg catcgagcca gccaaagggc aagccactta aaaataagat
    25141 gcgttgttcc gcaacggctt tcacgccctg ccagagagga tcacgacgaa taaaatctgc
    25201 agtaacggcc tcctgaacca gaataatatc cggctgccag cgcaggagat tttccatgga
    25261 aacctgtgtc aggccgtggc gatcggctat ttgcgcgacg ttgtgcaaac ctaataattc
    25321 cgccgcctcg gtatgcaacg aaccctgtaa gcccgtttcc agccctcggg gaccgcgcgc
    25381 agcataaaag cggaggttag cggcgggtga ggtggcgaat gcctgagctt caccaacgaa
    25441 gtggctggcg agattggctt gttctgcggc gcggtgctct tcgcctaacg ttttgccaag
    25501 cgttgttaac tgttctgctg attgcgccag ttttccgtta agcaataacc aggggatttg
    25561 tgtctgttcg ctaacctgcc gtgcctggga gatcaaggtt tcatccgtat tgccgcaatc
    25621 aacgaccaaa tcgggatgta acgccataag cccttccagc gatagtgtgc tggcgcgccc
    25681 ggcgagtcgt cccagcctgg gtaactggcg aatgtgctct ggcaatggaa ttaatgcctg
    25741 acgggcaaaa tcaaaagagg aaaaaccgac cattttttct ggcgcgacag ccagcaagag
    25801 caaatcggcg ggcgcgcctg cgctgacgat gcgtgtaatg ttttgggctt cgggaagaga
    25861 ttgcgaagca aaacgattaa ctgtttgtga aaaggacggt agagactgca ggagcagggt
    25921 agatgcaagt gcctgcgcaa acgtccgtct ggttatgggc atggagaaca tcctgaaaag
    25981 taaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat caaagtttag
    26041 ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct ctatcattaa
    26101 aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat catttaaata
    26161 tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt acttgtttta
    26221 taatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg tttcagtgtc
    26281 gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa cctatgttgt
    26341 tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt accgcaacaa
    26401 gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga agatatttat
    26461 tttcttagag agagtaattt actgaatgcg tttattcgga cgtgcaaaaa aagagatgat
    26521 aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta tgggaacaga
    26581 attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg gtgtagcaat
    26641 gataacattc tc
//
LOCUS       DADLDT020000056        25945 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000056 DADLDT020000000
VERSION     DADLDT020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.056"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..318)
                     /locus_tag="JQH22_002184"
     CDS             complement(<1..318)
                     /locus_tag="JQH22_002184"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2206495.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHK"
     gene            complement(451..756)
                     /locus_tag="JQH22_002185"
                     /pseudo
     CDS             complement(451..756)
                     /locus_tag="JQH22_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            824..1439
                     /gene="tnpA"
                     /locus_tag="JQH22_002186"
                     /pseudo
     CDS             824..1439
                     /gene="tnpA"
                     /locus_tag="JQH22_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1767..2849
                     /locus_tag="JQH22_002187"
     CDS             1767..2849
                     /locus_tag="JQH22_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFB2206496.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3338..4136)
                     /locus_tag="JQH22_002188"
                     /pseudo
     CDS             complement(3338..4136)
                     /locus_tag="JQH22_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4146..4697)
                     /gene="ybcL"
                     /locus_tag="JQH22_002189"
     CDS             complement(4146..4697)
                     /gene="ybcL"
                     /locus_tag="JQH22_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFB2206497.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4866..5198)
                     /gene="emrE"
                     /locus_tag="JQH22_002190"
     CDS             complement(4866..5198)
                     /gene="emrE"
                     /locus_tag="JQH22_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HFB2206498.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5543..5857)
                     /gene="fliE"
                     /locus_tag="JQH22_002191"
     CDS             complement(5543..5857)
                     /gene="fliE"
                     /locus_tag="JQH22_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFB2206499.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6071..7736
                     /gene="fliF"
                     /locus_tag="JQH22_002192"
                     /pseudo
     CDS             6071..7736
                     /gene="fliF"
                     /locus_tag="JQH22_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7729..8724
                     /gene="fliG"
                     /locus_tag="JQH22_002193"
     CDS             7729..8724
                     /gene="fliG"
                     /locus_tag="JQH22_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFB2206500.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8717..9403
                     /gene="fliH"
                     /locus_tag="JQH22_002194"
     CDS             8717..9403
                     /gene="fliH"
                     /locus_tag="JQH22_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFB2206501.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9403..10776
                     /gene="fliI"
                     /locus_tag="JQH22_002195"
     CDS             9403..10776
                     /gene="fliI"
                     /locus_tag="JQH22_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HFB2206502.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10798..11139
                     /gene="fliJ"
                     /locus_tag="JQH22_002196"
                     /pseudo
     CDS             10798..11139
                     /gene="fliJ"
                     /locus_tag="JQH22_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11136..12263
                     /gene="fliK"
                     /locus_tag="JQH22_002197"
     CDS             11136..12263
                     /gene="fliK"
                     /locus_tag="JQH22_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HFB2206503.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12368..12832
                     /gene="fliL"
                     /locus_tag="JQH22_002198"
     CDS             12368..12832
                     /gene="fliL"
                     /locus_tag="JQH22_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFB2206504.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12837..13841
                     /gene="fliM"
                     /locus_tag="JQH22_002199"
     CDS             12837..13841
                     /gene="fliM"
                     /locus_tag="JQH22_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFB2206505.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13838..14251
                     /gene="fliN"
                     /locus_tag="JQH22_002200"
     CDS             13838..14251
                     /gene="fliN"
                     /locus_tag="JQH22_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFB2206506.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14254..14619
                     /gene="fliO"
                     /locus_tag="JQH22_002201"
     CDS             14254..14619
                     /gene="fliO"
                     /locus_tag="JQH22_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HFB2206507.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14619..15356
                     /gene="fliP"
                     /locus_tag="JQH22_002202"
     CDS             14619..15356
                     /gene="fliP"
                     /locus_tag="JQH22_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFB2206508.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15366..15635
                     /gene="fliQ"
                     /locus_tag="JQH22_002203"
     CDS             15366..15635
                     /gene="fliQ"
                     /locus_tag="JQH22_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFB2206509.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15644..16429
                     /gene="fliR"
                     /locus_tag="JQH22_002204"
     CDS             15644..16429
                     /gene="fliR"
                     /locus_tag="JQH22_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HFB2206510.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16714..17337
                     /gene="rcsA"
                     /locus_tag="JQH22_002205"
     CDS             16714..17337
                     /gene="rcsA"
                     /locus_tag="JQH22_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFB2206511.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17381..17569)
                     /gene="dsrB"
                     /locus_tag="JQH22_002206"
     CDS             complement(17381..17569)
                     /gene="dsrB"
                     /locus_tag="JQH22_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFB2206512.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17732..17959
                     /gene="yodD"
                     /locus_tag="JQH22_002207"
     CDS             17732..17959
                     /gene="yodD"
                     /locus_tag="JQH22_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFB2206513.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18257..19072
                     /locus_tag="JQH22_002208"
                     /pseudo
     CDS             18257..19072
                     /locus_tag="JQH22_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(19069..20763)
                     /gene="yedQ"
                     /locus_tag="JQH22_002209"
                     /pseudo
     CDS             complement(19069..20763)
                     /gene="yedQ"
                     /locus_tag="JQH22_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20934..21116)
                     /locus_tag="JQH22_002210"
     CDS             complement(20934..21116)
                     /locus_tag="JQH22_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFB2206514.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21195..22112)
                     /locus_tag="JQH22_002211"
     CDS             complement(21195..22112)
                     /locus_tag="JQH22_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFB2206515.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22285..23205
                     /gene="yedA"
                     /locus_tag="JQH22_002212"
     CDS             22285..23205
                     /gene="yedA"
                     /locus_tag="JQH22_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFB2206516.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23194..23664)
                     /gene="vsr"
                     /locus_tag="JQH22_002213"
     CDS             complement(23194..23664)
                     /gene="vsr"
                     /locus_tag="JQH22_002213"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFB2206517.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23645..25063)
                     /gene="dcm"
                     /locus_tag="JQH22_002214"
     CDS             complement(23645..25063)
                     /gene="dcm"
                     /locus_tag="JQH22_002214"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFB2206518.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25130..25670)
                     /locus_tag="JQH22_002215"
                     /pseudo
     CDS             complement(25130..25670)
                     /locus_tag="JQH22_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25710..>25945)
                     /locus_tag="JQH22_002216"
     CDS             complement(25710..>25945)
                     /locus_tag="JQH22_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206519.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6534 a         6646 c         6388 g         6377 t
ORIGIN      
        1 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
       61 tttctgcttc tgccagttgt tgctgaattt gaccttgcgg cttaactgtc gctgaagtct
      121 cgccagcttc ttctggtttt tctgaaaact gtttacaggc tcaaagactg tgccatctga
      181 cagcgaggca agtttagcca cgccagcatc cagcccgacc attgatgctg aagggtgaac
      241 cggagttgat acttcacttt ctgcctgaat actgatgtac cacttaccgc aggactggct
      301 gacagtgaca tttttcacaa cacccgtgac ctgacggctg ttccggtagc gcatccagcc
      361 aagttttggc agaaaaatac ggctgttttc ctgagcgagc ttaacgccct gcggatagcg
      421 gaatgcatca ttctgtcacc gctttttgaa tcaggggaac gccgagcgct tctggaaaaa
      481 gtttttgcag gcctgctcag ggtctttcag tgactgttgc aatggctgtg acggagcatc
      541 tttaatccat tgcgtttcag tggcattttt ccactcaacc aatcaggaag ccattttcgc
      601 gtaagggatg tctttgttac cggccttatg attctcattc tgacgtgcca gagtacgact
      661 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg
      721 tcttaaccgg aatttgaata cctgtagtcg tttcacaggg caaattatac tttggtctgt
      781 gaaaaaaact gatatccgac gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt
      841 ctttgttacc aagtaccgac gccagatttt tgaccatgac gcgacagaaa aactacgcac
      901 ttacttttca aatgtatgtg cttattttga agctgaactg gttgaaatgg atggcgaacc
      961 agatcacgtc catctgttaa ttaactatcc ccccaaactg gcgatatcca gtctggtaaa
     1021 caaacagcct caaaggcgta tcgggtaggt tactgcgacg ggatcgacca gatattgctg
     1081 ccaggtatta ccgcaaagat gttttgtggc gtcccggcta ttttgccagt agctgcggag
     1141 gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcagatga
     1201 aaaaacgagc cttatatccc cgccctgaag gacgggcttt tacggtgcac tggataagcc
     1261 tggagattat ctacacctgc atggacatca ttgtactatg ccccgtacat gccccatcac
     1321 atcctcaaca tcaaagtatt agagccttgc aaaccattat gcgtctcagt ttgtctcatc
     1381 tatcttacgc aaaaaaaata gaactaatat caagcggcgc tatattgcgt attaaataaa
     1441 accacctcaa ttacattaat aaatctaaat aaatatcatg caaataattt ccatggttat
     1501 ttttaggcta tttaaattaa aaaaataaat tatgagtcaa tcataaataa caacctttaa
     1561 tataatttga ctaccaaatt tatattcgtt cacaaaatag tcatgcattg tgtgcaatag
     1621 aaacagttca gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac
     1681 atcttgaaat aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa
     1741 taatagttca aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
     1801 ctgcatcagt actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta
     1861 acaagctgga tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag
     1921 atgatggcga cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc
     1981 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag
     2041 gttcttccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
     2101 ccatcgatta tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc
     2161 tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca
     2221 caggtgttgc aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
     2281 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt
     2341 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     2401 ctgaccgcac taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     2461 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     2521 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     2581 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     2641 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata
     2701 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     2761 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     2821 ttgctgtagg attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc
     2881 tttgcctccc caacatataa gtggcgctct caagccactt tccttagaag cactatcttg
     2941 cttctgacta tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata
     3001 atactcagtg aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac
     3061 ctgatatcta atgtcataag catcccttta attatcccct caacctcttg taaatttccc
     3121 actcttgagc tccacatagc ccaaacgccg gatgcaaaaa aaatcacacg tgcatctctg
     3181 aaattgtgat ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca
     3241 cagaagtcac cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa
     3301 ataaaaatcg atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta
     3361 gctctcttgg tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact
     3421 tgtaaccatt ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac
     3481 aacgcattat ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc
     3541 agacggcgat gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc
     3601 cattttgttc taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta
     3661 ctatctgata aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt
     3721 gctaagtgtt cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc
     3781 caacaatata ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga
     3841 tcagttatcg cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt
     3901 tctgcaagat aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga
     3961 aataatatta tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt
     4021 tttccctgac ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga
     4081 tgtttatgca tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc
     4141 ttaccctatt ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta
     4201 agcatataac caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt
     4261 agagcccata ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca
     4321 ccaaatcctg cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta
     4381 ccatcacgtc ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca
     4441 gtccagtgcc accatccact gcctgtagga gcatctggat catacacagt tacggcaaag
     4501 cttttggtgc cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca
     4561 cacccaaatc cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta
     4621 ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa
     4681 acgataagtt ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg
     4741 ccttgatctc attatgaata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag
     4801 gtgccccgaa cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt
     4861 attttttaat gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc
     4921 aacatcatgc ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc
     4981 aaactaatca gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata
     5041 taagccagcg tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca
     5101 gagggccata accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct
     5161 gcaagtattg cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata
     5221 ctggagagaa ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt
     5281 atccaagggg ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac
     5341 cccaggctaa cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc
     5401 gcgtttacgg atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga
     5461 aattgagtga gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag
     5521 catagtcaac aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca
     5581 ccagcttatt acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg
     5641 tcatcacatc gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc
     5701 gggcgactgt ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac
     5761 taatggtcgg ttgcggcagt gattcctgcg cacacgcact catcgccgta gcctgtaact
     5821 ggctgataac cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg
     5881 gtaacaaagc ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg
     5941 gtttattggc ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtt
     6001 tgctgacccg agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac
     6061 gaggtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct
     6121 gcgtgcgaat ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt
     6181 cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga
     6241 tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag
     6301 cgaagccagc ggcgctattg aggttccggc agataaagtt cacgaactgc gcctgcgcct
     6361 ggcgcaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa
     6421 gtttggtatc agccagttca gcgaacaggt gaattatcag cgggcgctgg aaggcgagct
     6481 ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc
     6541 gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga cggtaaatct
     6601 gttacccggc cgcgcactcg atgaagggca aattagcgct attgtacatc tggtttccag
     6661 cgcggttgct ggtctgccgc ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt
     6721 aacccagtcc aataccagcg ggcgcgatct taatgacgct cagttgaaat atgccagcga
     6781 tgtcgaaggc cgtattcagc agcgtattga agcgatcctg tcacctattg ttggtaacgg
     6841 taatattcac gcctaggtca cggcgcaact ggacttcgcc agtaaagaac aaacggaaga
     6901 acagtatcgc cctaacggtg atgaatctca tgcggcgctt cgttcacgcc agcttaatga
     6961 gagcgagcaa agcggttccg gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc
     7021 ggcacctgcg aataacgcgc caatcagcac gcctccgaca aatcaaaata accgccagca
     7081 gcaggcgagc accaccagca atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa
     7141 ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct
     7201 gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccat tgcctctcag
     7261 caacgaacag atgaagcaaa ttgaagctct gacccgcgag gcgatgggct tttctgaaaa
     7321 acgcggtgat tcgctcaatg ttgttaactc gccgttcaat agcagtgacg aaagcggcgg
     7381 agcactgccg ttttggcaac agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg
     7441 gttgctggtt gctggtactg ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc
     7501 agctaacacg tcgcgccgag gcggtgaaga ctgtgcagca acaggcgcag gcccacgagg
     7561 aagtggaaga tgcggtggaa gtccgtctga gcaaagacga acaactccaa caacggcgcg
     7621 ctaaccaacg actgggggca gaagtcatga gccagcgtat ccgtgaaatg tctgataacg
     7681 atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat gagtaacctg
     7741 acaggcaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg ggcggcagag
     7801 gtgttcaagc acctctccca gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc
     7861 acgcagatct ccaacaagca gctaaccgat gtgctggcgg agtttgagca agaagctgaa
     7921 cagtttgctg cactgaatat caacgccaac gattatctgc gttcggtatt ggtcaaagct
     7981 ctgggtgaag aacgtgccgc cagcctgctg gaagatattc tcgaaacccg cgataccgcc
     8041 agcggtattg aaacgctcaa ctttatggag ccgcagagcg ccgccgatct gattcgcgat
     8101 gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca ggccgccgat
     8161 attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt
     8221 ggcggcgtgc agccagccgc gctggccgaa ctgaccgaag tactgaatgg cttgctcgac
     8281 ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga aattatcaac
     8341 ctgatgaaaa cgcagcagga agaagccgtt attaccgccg tgcgtgaatt cgacggcgag
     8401 ctggcgcaga aaatcatcga cgagatgttc ctgttcgaga atctggtgga tgtcgatgat
     8461 cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa
     8521 ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat
     8581 attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa
     8641 cagaaagcga ttctgctgat tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc
     8701 agcggcgagg atacctatgt ctgataatct gccgtggaaa acctggacgc cggacgatct
     8761 cgcgccacca ccggcagagt ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga
     8821 ggccgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc acgagcaggg
     8881 ttatcaggcg gggattgccg aaggtcgcca gcaaggtcac gaacagggct atcaggaagg
     8941 actggcccag gggctggaac aaggtctggc agaggcgaag gctcaacaag cgccaattca
     9001 tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt
     9061 gatagcttcg cgcctgatgc agatggcgct ggaggcagca cgtcaggtga tcggtcagac
     9121 accaacggtg gataactcgg cactgatcaa acagatccaa cagttgttgc agcaagaacc
     9181 gttattcagc gggaaaccac agctgcgcgt acacccggat gatctgcaac gtgtggatga
     9241 tatgctcggc gcaactttaa gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca
     9301 ccctggcgga tgtaaagtct ccgccgatga aggcgatctc gacgccagtg tcgccactcg
     9361 ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact
     9421 cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt
     9481 cgctacgggc gattaacccg cgctaccggg ctggtgctgg aagccaccgg attacaattg
     9541 ccgctcggcg caacctgtgt cattgagcgc cagaacggca gcgaaacgca cgaagtagaa
     9601 agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa
     9661 ggtgtcctgc ccggcgcgcg tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc
     9721 ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa
     9781 ccgctcgatg gcctgccctt ccccgatacg acggaaaccg gtgcgctgat taccccgcca
     9841 tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc
     9901 aatgccctgc ttaccgtcgg acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt
     9961 ggtaaaagtg tgctgctggg gatgatggca cgctacaccc gcgccgatgt cattgtcgtg
    10021 ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta ttgagaatat cctcggtgcc
    10081 gaaggacgtg cacgctcagt ggtgattgcc gctccggcgg atgtttctcc gctcctgcga
    10141 atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat
    10201 gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg
    10261 gcgattggcg aaccacctgc cactaaaggt tatccaccgt cggtgtttgc caaattaccg
    10321 gcactggtcg agcgtgccgg taatggcatt agcggcggcg gctcgattac cgcgttttat
    10381 accgtgctca ccgaaggcga tgaccagcag gaccccattg ctgactccgc gcgggcaatc
    10441 ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc
    10501 gatattgaag cgtcgatcag tcgcgcaatg acggcgttga tcagtgagca acattacgcg
    10561 cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc
    10621 gtcggcgcgt atgccaaagg cagcgatccg atgctcgata aagccatcgc cctgtggccg
    10681 cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc
    10741 caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggcg
    10801 gaagaacagc tcaaaatgtt gatcgattat cagaatgaat atcgcaataa cctcaatagc
    10861 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
    10921 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
    10981 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
    11041 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
    11101 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta
    11161 ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
    11221 ttctcacgtt gttgagcgaa gcattagcag gcgagacaac aactgacaaa gcggcccccc
    11281 agttgctggt agcaacagat aagcccacga caaaaggcga gccgctggtc agcgatattc
    11341 ttgccgatgc gcaacaagcg gatttactga tccctgtgga tgaaacactt cctgtcatca
    11401 acgacgaaca atccacatca acaccgttaa ccaccgctca gacgatgacg ttggctgcgg
    11461 tggctgacaa aaatacgaca aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg
    11521 caagtctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
    11581 atgcaccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
    11641 cgcaactcac tacagcacag cctgatgacg cccccggcac accagctcag ccattaacac
    11701 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg
    11761 ctgacgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
    11821 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattgagc cagcatattt
    11881 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcaccca caggatttag
    11941 gtgaagtgca aatttccctc aaagtggatg ataaccaggc gcagatccag atgatttcac
    12001 cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
    12061 ccgaaagtgg cattcagtta gggcaaagca acattagtgg cgaaagcttt agtggtcagc
    12121 agcaggccgc ttcccagcaa cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg
    12181 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggtaaca
    12241 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
    12301 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
    12361 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
    12421 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
    12481 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc
    12541 gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac
    12601 cctgcgactg aaagatgaag caacccgctc gcggctgagt gagtatttgc cggaagtccg
    12661 tagtcgcttg ctgttactgt tttcacgtca ggatgctgcc gtactggcga cagaagaagg
    12721 caagaaaaac ctgattgccg agattaaaac cacactttcc accccgcttg ttgccgggca
    12781 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
    12841 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
    12901 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
    12961 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
    13021 cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccggac attaccgtcg
    13081 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
    13141 tgaacctaat ccacctgaaa ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc
    13201 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
    13261 aaggtcgcga gtttacccat actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc
    13321 tggaaggcta tagcgacgcc tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt
    13381 cggaaatgca ggtggaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
    13441 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc ctgccattca
    13501 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    13561 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggaactgg
    13621 tggccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
    13681 acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggttc
    13741 tgaccagtca gtacggtact ctcaacggtc agtatgcgtt acggatagaa catttgatta
    13801 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    13861 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc
    13921 aaccagcgaa aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    13981 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct
    14041 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc
    14101 gctggacggt ctggcgggcg aaccgctgga tattctgatc aacggttatt taatcgccca
    14161 gggcgaagtg gtggttgttg ccgataaata tggcgtgcgg atcaccgata tcattacccc
    14221 gtccgagcgg atgcgccgcc tgagccgtta gtgatgaata accatgctac tgtgcaatct
    14281 tccgcgccgg tttctgctga gccactgctg caggtgagcg gcgcactcat cgccattatt
    14341 gccctgatcc tcgctgctgc ctggctggta aaacggctgg gatttgcccc taaacgcact
    14401 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg
    14461 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    14521 ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagaccga ttttcagtcg
    14581 gtcatgaaaa atttgcttaa gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg
    14641 caccggtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg ggtatcacca
    14701 gccagccgct acctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    14761 tcacctcgtt gacgttcatt ccagcgattt tactgatgat gaccagtttc acccgcatca
    14821 ttattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg
    14881 tattgctggg gctggcactg tttttgacct tttttattat gtcgccagtg atcaacaaaa
    14941 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg
    15001 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgcgag gcagatttag
    15061 ggttgtttgc cagactggcg aataccggcc cgttgcaggg gcctgaagct gtgccgatgc
    15121 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    15181 cgattttcat cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    15241 ggatgatgat ggttcccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    15301 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    15361 gcaaaatgac acctgaatcg gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg
    15421 cactggcagc cccgctgttg ctggtagcgc tggtcacggg ccttatcatc agtattttgc
    15481 aggcagccac gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat
    15541 ttatcgccat cattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    15601 ccttgttcac taacctgccg tatatcatcg ggtagcccgt acaatgatgc aggagacaag
    15661 cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    15721 catttctacc gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct
    15781 ggcaatgatg atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt
    15841 ttcgttcttt gctctgtggc tggccgtaca gcagatcctg atcggcattg cgcttggttt
    15901 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat
    15961 ggggctgtca tttgcgacat ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc
    16021 gcgcatcatg gatatgctgg cattactgct gttcctgaca tttaacggtc atttatggtt
    16081 gatttcactg ctggtcgata cctttcacac cctgccgatt ggtagcgaac cgttgaacag
    16141 caatgcgttt ctggcaccca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct
    16201 ggcgttaccg ctcattactc tgttgctgac cctgaatctg gcattaggtt tactgaatcg
    16261 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggcat
    16321 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    16381 aatttttaat ttgctggctg atattattag tgaattacca ttaatataat tccgtaacgt
    16441 ttatcatgct accctaagga ttatccgaaa aataatatct gcgaacatct tccaggatac
    16501 tcctgtagcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttaagaca
    16561 attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa aattgcgaga
    16621 gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt caggtaaggg
    16681 gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat ggatttatgt
    16741 agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa aaaaagagaa
    16801 atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca gcgtccttca
    16861 gtggtgttta ttaatgagga ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag
    16921 cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat tgccaatgtt
    16981 cattttgatg aatatctatt ggtcagaaaa aatttattga tcagttctaa atcgattaaa
    17041 ccggaatctc tcgacgatat ccttggcaat attctgaaaa aagagacaac gataacctcg
    17101 tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg
    17161 atggcaggtc agggaaccat tcaaatctcc gaccaaatga atatcaaagc caagaccgtt
    17221 tcatcgcata aaggtaatat taaacgtaag atcaaaacgc ataataaaca ggttatctac
    17281 catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat gcgctaacac
    17341 attctgactg gtgatttccc accagtcagg ctgaataaga ttactctgct ttctcaacaa
    17401 agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg tagtcttcca
    17461 gcgcaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc actccaggac
    17521 gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc gttttcctcc
    17581 tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag
    17641 tgctgatttt ctcgacggtc tatacttaag agatgctagg cggacttaac gactggcggc
    17701 aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac
    17761 cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca atgaaattag
    17821 cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg atggacgtga
    17881 atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg acttcagcgt
    17941 ctctgaagtg aatcgttgaa ttcacaataa aaaaatcccg accctgaggg tgtcgggatg
    18001 aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc
    18061 atcaccttat ctgcaatttt tttcgctgac aagaaaatat tcgcgctatg aatgattaac
    18121 gctacttatg aatagaaatg tgacgcgcgt caccccttgg tggctctggt gtcaacggga
    18181 tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat cctggtaagg
    18241 ggagtactgc gcgaccatgt tttcaattca acaaccacta ctggttttta gcgatcttga
    18301 tggcaccctg ctggacagtc atagttatga ctggcaaccg gcagccccct ggctcagccg
    18361 tttacacgaa gccaatattc ccgtcattct ctgtagcagt aaaactttag cggaaatgct
    18421 gtacttgcaa aaaatgttgg ggctacaagg cttaccgctg attgcagaga atggcgcagt
    18481 gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca tctcaggtat
    18541 tagccatggc gaaatctgcc aggttttaaa tacgctacgc gagaaagaac attttaaatt
    18601 cacgactttt gatgatgtcg acgatgcaac cattgccgaa tggacgggat taagccgtag
    18661 ctaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga
    18721 cgagcgtatg gcacaattta tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg
    18781 tgcgcgcttc tggcacgtac tggatgcctc tgccggaaaa gatcaggctg ccaattggat
    18841 tatcgcgacc tatcaacaat tgtcaggcag acgcccaacc acacttggcc tgggcgatgg
    18901 gccaaacgat gcgcctttac tggaggtaat ggattacgcg gtgattgtga aagggctaaa
    18961 ccgtgaaggg gtacatctgc atgatgagga tccggcccgc gtctggcgaa cgcagcgtga
    19021 aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgcccgtt aagcgttatc
    19081 gctcgcgaat acccgattgc gcccggcctg ttttgcgagg taaagccgac ggtcggccaa
    19141 tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta cccccagcga
    19201 ggcactgatg cgtatcgttg tactcttggc gatcagcatc tctttttcat ttaacttcag
    19261 gcgaatacgt tctgcgacct ccgcagcctg cgtcagattc gcgcctggca gaatcacaca
    19321 aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa
    19381 tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc
    19441 tttaaaatgg tcaagatcga cctgaatgac agaaaaagga tgttggtgcg tctgacacaa
    19501 tttagcgagc ggacgggctt tttcgaacag tgcgccacgg ttatataaac gcgttaaggt
    19561 gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc tgaccatccg
    19621 gcgaatcaca taccaggaga ggagtaacat ggtggtaaag agcgcccaca gcagggttaa
    19681 tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc
    19741 cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt ccatacgaat
    19801 gccgccccgc gtgtcatgtt ccattgcctg cgccagcaag gccagttcac gaggatcaaa
    19861 aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct tactgtcata
    19921 gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt gctgcatagt
    19981 acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt ctaccggaac
    20041 actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg
    20101 taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt gggtgacata
    20161 gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa
    20221 tccagcacgc gagacataca tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg
    20281 cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt cattttcgcg
    20341 agacaggaaa ttcccctcgc tgactaatgc atccgatacg ccattgactg gcagggtgcg
    20401 ccgtcggttg agttcgattt gccaggcgtg ctcgtcgcga tgctgttcga actcggttac
    20461 ggcattacgc agagaggtga aatccagtgg cgccacgaga gcttcgcgca tgccattacg
    20521 caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt tggctacgtt
    20581 ttccagatga ttacgctagc tggagatata ggcatcttcc agcaccacca cttcacgcca
    20641 ggtgagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga catgaccggg
    20701 ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg tctcgtgctg
    20761 caccctgacg actcccaacc tgctttttta tgattctggc ataacccggc aatagcgtac
    20821 cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc attcattgca
    20881 ggcattggga caccaggccg gatgtgatcc ggcctggtgt taatgatacc ctttcaaact
    20941 tcttccgcag aacgactccc ctcccccgga acaagatcgg tttcatgaaa agcttcccgc
    21001 ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta tccagtgaca
    21061 atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa gctcatactc
    21121 gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat tttgcataat
    21181 agccagtgcg agtattaatg tgcctgaccg cgcattttcg ctactgcttt cactcccagc
    21241 accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg taatatcatc
    21301 gccaccactg cactttgctg cccggcgata tgttcaatag cgtgatgcag cggcgcaata
    21361 ccatgtacca caatcccgcc gccgacgagg aacatcgcca gcgtgccgac aatcgataat
    21421 gctttcatca gccaaggcgc gataatcaat aaccctttac ctaatgcctg catcagcgcg
    21481 ctagattttt ccgccagcca ataccctagg tcatcaatct taacgataac ccctaccaga
    21541 ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac ctgattaagc
    21601 aacggcgcct ctgccacaat ccccagcgtg atggcgacga tttccgcaga caaaataaaa
    21661 tcggtacgaa tcgccccttt aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc
    21721 agcttctcca gacgctgctg gctctgcgct ggatcttctt tatgtttacg cgcctccagc
    21781 atatgcagca ctttctctac cccttcaaag cagagaaacg cgccaccaat catcaacagt
    21841 ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac cagaatcact
    21901 ttattgatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg gttggcccgc
    21961 acgcctgaaa cttgttgcgc attgagcgat aaatcatccc ctaatacacc ggcggttttc
    22021 ttcgccgcca gtttgcccat cacagagata tcgtccagca gtgtggcgat atcatcgagc
    22081 aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg ttgaagggtg
    22141 taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca tttatttaac
    22201 aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact atgaaccacc
    22261 tttttatttc cgccgtgagg gattatgcgt ttccgccagt tgttaccgct ttttggcgcg
    22321 ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat tggcgtggga
    22381 agctggcctc cgttaatgat ggcgggcgtt cgattccagg cagccggtat tttattgctg
    22441 gcatttttgc tactgcgcgg acacaaactt cccccgctac gtccgctgct caatgccgcg
    22501 ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa
    22561 aatgttcctt ccggcatcgc cgccgtagtg attgcaaccg tgcccctctt taccctgtgc
    22621 ttcagtcgcc tgtttggcat taaaacgcgc aaactggaat gggtgggtat tgccattggg
    22681 cttgccggaa tcatcatgct caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg
    22741 attctgattt taatcggctc gattagctgg gcgtttggct cagtttatgg ctcgcgcatt
    22801 accttacctg tagggatgat ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta
    22861 atgatcgcat cgatgattgc gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc
    22921 cttgcggtcg gctatctggc gctgtttggt tcgattatcg ccattaacgc ttatatgtat
    22981 ttaatccgta atgtcagtcc ggctctcgcc accagctacg cctacgttaa cccggtggtc
    23041 gcggtcttgc tgggtacggg actgggtggc gaaacgctgt cgaagattga atggctggcg
    23101 ctcggcgtaa ttgttttcgc ggtggtactg gtcacgttgg gaaaatatct cttcccggcc
    23161 aaacccgttg tcgcgccagt tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt
    23221 gtcgatctgc gcgctggcac cttcgccgca gatccactct tccagacgct cggtaagcgc
    23281 cgcatccgtc agcttctcgc gcctacgtaa cgcacactcc cagacaatca gtacgcgcca
    23341 gccgagctcc tgcaagcgac tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt
    23401 ctccagccag aactcagttc gagtcgcagg cactttaaac agatagcagt gatgatgatg
    23461 ccagaagcag ccgtgggtaa atatcacgca gcgatattca tcaacgacaa aatccgggcg
    23521 tccgggcaga ctggcgtcct gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc
    23581 gaggcgcttc tcaatcgccg tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt
    23641 ggccttatcg tgaacgtcga ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt
    23701 tgatttttgg ctcaagcagt tttgccaccg cggcaaagac cggcacgacc accgagttgc
    23761 cgaactggcg ataggcctga gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg
    23821 cttcaaaacc cattaagcgc gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct
    23881 gattcagcgg atcgtcaaag tctttctcac ccgtggccat atcccaaccg cgatcgatta
    23941 aaatttccgc gccatctttg taataacgag cagacagcgt acgggtgacg ctttgcggat
    24001 tgttcggata aaccattcca taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat
    24061 atcgatagag gtacttccac agcaccggcg tcaggatata tttcgcctca accatcgggt
    24121 ccagcagctg cgccagcgtc actcgctgtg cagggaaaca ttcgctgata tcacgcagag
    24181 taaaatcggc tttcagattc agatcgcgac gaaaacccac cagcacgatg cgttcacggt
    24241 gctgcggcag gaaatgttta ccatcgatga ttttcggatc gtccggcccg ttatcttctg
    24301 catcggcaac gtcatagccc agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc
    24361 ccttatcgtg actcttcagg tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg
    24421 cgtcgataat gcgcaccaca tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt
    24481 gcgcccgccc gagcgagttc tttttcgata cgccagccag cgaaaatggc tgacaaggga
    24541 aaccggccag taaaacatcg tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct
    24601 catcactcac gccttcttga tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat
    24661 gcgtcgccgg gtcgcaataa tggttggctt tataagtgcg taccgcatgt ttgttccatt
    24721 cgctggtaaa cacgcactgt ccaccaatcg attcaaaacc acgacggatg ccgccaattc
    24781 cggcaaatag atcgataaag cgaaacgcat aatgcggatg atgttccggt ggtttgggta
    24841 acaacgtttg cagatgggca aactctttct cacttaaacg gtgccatgcc gagtcattcg
    24901 ccagcgcacg ttttaaaatt gccgcgctcc agtgattctc acccacacca ttaagctgcg
    24961 ccaccagcat tttaacatca taaatttgca gcagtttctc cagcattgcc tgtgcggcat
    25021 tcccggtgct gtatgaatcg gttactgata tattttcctg catagattta accggccatc
    25081 tgagatggcc gacaggttaa cataattact gccgtttagg gccacgcgct cagccagcgg
    25141 gtgaaaacgc atctatcacc ttgtgatcga taccttcatt ctcccccgcc agttcggcac
    25201 cgagcttcgc catcaaacag cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc
    25261 aggccaatcg cccccagggc ttccagccga tccgcatcct ggacgatttt agcttccgtt
    25321 gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca aacggcctca
    25381 attttctccg ccggaaattg cacgaactct tcacggagca ggcgacgcgt ctcttctgcc
    25441 gccaggattg aagaacgctg ccgttgcgga tgatttttcg ccaggctcac gatatcgtga
    25501 aaataacagg cggttaaaat caccagcata tcgacatcgt catctgccgc cagtttctgt
    25561 gccgtagccc agacacggcg aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga
    25621 tggtttttca accagttctc gaactgtgcc tgccagtgtt gtaactccat acgcgctccg
    25681 ttgccaaaga gtcgcaacct tagcagtttt tattcatagc gtctgcaacg tgcgggacga
    25741 ggtcgggtgt accatgcaat gccgcctaac aaggcaccaa ccagggccat aaacagaaat
    25801 ccgaccaatg ctcctaacca cttccccgcg gtagatccta aatcactttc aaatcccgac
    25861 acgggggcat cggggatccg gctcatcacc cagatgtatc gcgccattgc ccagacgaaa
    25921 tagccacacc atgtatagaa agccc
//
LOCUS       DADLDT020000057        25705 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000057 DADLDT020000000
VERSION     DADLDT020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25705
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.057"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..921)
                     /locus_tag="JQH22_002217"
                     /pseudo
     CDS             complement(1..921)
                     /locus_tag="JQH22_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            1216..1890
                     /locus_tag="JQH22_002218"
     CDS             1216..1890
                     /locus_tag="JQH22_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFB2206520.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1929..3590)
                     /locus_tag="JQH22_002219"
     CDS             complement(1929..3590)
                     /locus_tag="JQH22_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFB2206521.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3786..4214)
                     /gene="ibpB"
                     /locus_tag="JQH22_002220"
     CDS             complement(3786..4214)
                     /gene="ibpB"
                     /locus_tag="JQH22_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HFB2206522.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4326..4739)
                     /gene="ibpA"
                     /locus_tag="JQH22_002221"
     CDS             complement(4326..4739)
                     /gene="ibpA"
                     /locus_tag="JQH22_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HFB2206523.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(4733..4805)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            5044..5376
                     /locus_tag="JQH22_002222"
     CDS             5044..5376
                     /locus_tag="JQH22_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFB2206524.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5378..6598)
                     /locus_tag="JQH22_002223"
     CDS             complement(5378..6598)
                     /locus_tag="JQH22_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFB2206525.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            6699..7799
                     /gene="cbrA"
                     /locus_tag="JQH22_002224"
     CDS             6699..7799
                     /gene="cbrA"
                     /locus_tag="JQH22_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HFB2206526.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(7760..9097)
                     /locus_tag="JQH22_002225"
     CDS             complement(7760..9097)
                     /locus_tag="JQH22_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206527.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9172..10320)
                     /gene="dgoD"
                     /locus_tag="JQH22_002226"
                     /pseudo
     CDS             complement(9172..10320)
                     /gene="dgoD"
                     /locus_tag="JQH22_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10317..10934)
                     /gene="dgoA"
                     /locus_tag="JQH22_002227"
     CDS             complement(10317..10934)
                     /gene="dgoA"
                     /locus_tag="JQH22_002227"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFB2206528.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10918..11796)
                     /gene="dgoK"
                     /locus_tag="JQH22_002228"
     CDS             complement(10918..11796)
                     /gene="dgoK"
                     /locus_tag="JQH22_002228"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFB2206529.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(11793..12482)
                     /gene="dgoR"
                     /locus_tag="JQH22_002229"
     CDS             complement(11793..12482)
                     /gene="dgoR"
                     /locus_tag="JQH22_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFB2206530.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12760..13416
                     /locus_tag="JQH22_002230"
     CDS             12760..13416
                     /locus_tag="JQH22_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206531.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13462..14274)
                     /gene="yidA"
                     /locus_tag="JQH22_002231"
     CDS             complement(13462..14274)
                     /gene="yidA"
                     /locus_tag="JQH22_002231"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFB2206532.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14388..14786)
                     /locus_tag="JQH22_002232"
     CDS             complement(14388..14786)
                     /locus_tag="JQH22_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFB2206533.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(15026..17440)
                     /gene="gyrB"
                     /locus_tag="JQH22_002233"
     CDS             complement(15026..17440)
                     /gene="gyrB"
                     /locus_tag="JQH22_002233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFB2206534.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17469..18542)
                     /gene="recF"
                     /locus_tag="JQH22_002234"
     CDS             complement(17469..18542)
                     /gene="recF"
                     /locus_tag="JQH22_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFB2206535.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18542..19642)
                     /gene="dnaN"
                     /locus_tag="JQH22_002235"
     CDS             complement(18542..19642)
                     /gene="dnaN"
                     /locus_tag="JQH22_002235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFB2206536.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19647..21050)
                     /gene="dnaA"
                     /locus_tag="JQH22_002236"
     CDS             complement(19647..21050)
                     /gene="dnaA"
                     /locus_tag="JQH22_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFB2206537.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21347..21427
                     /locus_tag="JQH22_002237"
     CDS             21347..21427
                     /locus_tag="JQH22_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206538.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21657..21797
                     /gene="rpmH"
                     /locus_tag="JQH22_002238"
     CDS             21657..21797
                     /gene="rpmH"
                     /locus_tag="JQH22_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFB2206539.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            21814..22173
                     /gene="rnpA"
                     /locus_tag="JQH22_002239"
     CDS             21814..22173
                     /gene="rnpA"
                     /locus_tag="JQH22_002239"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFB2206540.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            22137..22394
                     /gene="yidD"
                     /locus_tag="JQH22_002240"
     CDS             22137..22394
                     /gene="yidD"
                     /locus_tag="JQH22_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFB2206541.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22397..24043
                     /gene="yidC"
                     /locus_tag="JQH22_002241"
     CDS             22397..24043
                     /gene="yidC"
                     /locus_tag="JQH22_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFB2206542.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            24149..25513
                     /gene="mnmE"
                     /locus_tag="JQH22_002242"
     CDS             24149..25513
                     /gene="mnmE"
                     /locus_tag="JQH22_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFB2206543.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6240 a         6935 c         6534 g         5996 t
ORIGIN      
        1 acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca
       61 ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagt
      121 ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct
      181 tcaacagctg ccggaccaaa gataaacggt ccgtagatga agtggtgtaa accggttggg
      241 atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc cgaacgcagg
      301 aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa tgtcagccag
      361 gcacagggga tcatcaccag gaaggcgata ctcaccacat aagacgtccc ctggaaaatg
      421 ccgagaaaaa ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg
      481 gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccggc catcattgtc
      541 agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct tccccaggtc
      601 attcccatcg cattgatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa
      661 gcacgtccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc
      721 atattacgga ataccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa
      781 ctgttcggat cggtcagtga ttccccgaca aaacatcggg ttttgcagca agatggcaag
      841 acccaccaca atcccggcga agggaaacag cagcactggc gtgaacatcg cgccgccaaa
      901 gcgttgaatt tgactgagca tttccacatc ctcttttctc aattctgtaa gtaggaagcc
      961 tgattggtta tgcggcagac ctggagcgag gataggaaga tgcgatcggc agaacatgac
     1021 gacccaatgg gattcgtgat cgctttcatg cttttcgttg ggcggtagag atctacgttt
     1081 tcgcgaaaaa gtagacatct gtgagcacag gtacacagca tcgaacacat ctttaaaaaa
     1141 aagatttttt tcaatcgatt aagcagaact tgtgggcgca ttacccgtgc ttgcaggcaa
     1201 aaaagagagc tagagatgat ctacaaaagc attgcggagc ggttaagaat tcgacttaat
     1261 tccgcggatt tcacgctaaa cagccttctt cccggtgaaa aaaagctggc ggaagagttt
     1321 gcggtatcgc ggatgactat ccgtaaagcc attgacctgc tggtagcgtg ggggctggtg
     1381 gtccgccgcc acggcagcgg cacttacctg gtgcgcaaag atgtgctgca tcaaaccgcc
     1441 agcctgaccg gactggtgga ggtgttaaaa cggcagggaa aaacggtcac cagccaggtg
     1501 ctgatttttg aaatcatgcc tgcgcctccg gccattgcca gccagttacg gattcaaatc
     1561 aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc gctaatgctg
     1621 gaagacagct atatgccggt aaaactgttc cgtaaccttt cgctgcaaca tctggaaggg
     1681 tcgaagtttg aatatattga acaagagtgc gggattttga ttggcggtaa ttatgaaagc
     1741 ctgatgccgg tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg
     1801 ccactgctgc ggatcacctc gttgtcatat agcgagagcg gggagttttt gaattattca
     1861 gtgatgttca gaaatgccag cgaatactag gtggagtacc atttacggcg actccacccg
     1921 gaaaaaagtt aaccgatact ccagaagagc accgccagta attggggggt gataatgcgc
     1981 aggaacatca ctaacggata gacagtggcg taagagagcg ccgccgcacc actggttgga
     2041 tgaagattat tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg
     2101 cacatggtca ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc
     2161 ggaacggcgg tgatcagggc accataacca atccagctta gcccttcgcc attgaccagg
     2221 gtattcacaa aatccccacc agatttcaga ccaacgaccg agaggaacag cacgatcccc
     2281 agctcccgca gcgcgaggtt ggcacttggc ggcataaacc agtacagctt gccgatactg
     2341 ccgatacgcc cgaggatcaa cgccataatc agcggaccgc ccgccagccc cagtttcaac
     2401 gcggccggga atcctggcac aaagacggga atagaaccta acaatacgcc aagcccgatg
     2461 ccaataaaca ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca
     2521 ttggcaacgg catcaattgc ggacggacgc cccaccaggt tgaggatatc gccgaactgc
     2581 aggctgatat cgccactggc gaccagttcg accccggcac ggttcaggcg cgagataaca
     2641 acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac gttttcattg
     2701 gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg tagacagcga ggtatcgacc
     2761 tcctgaccaa tcaccagttg cgcattatgt aaatccgctg gctgccccac cagatgcagc
     2821 aaatcgccca gttggataat ggtatctggc gaaggtactt ttagggtttc ttctcgtttc
     2881 agacgcgagc agataatttt gtcgccgttg agaatcggta catctttaat ggctaaatca
     2941 tgcaggttag ggttctcaac gcgaatattg atagtcttga tcaacgcgcc gccattggtg
     3001 cgtgaagact cgtgctgctg agcttctgtc tcgacattga cgcggaaaat aacccgcaac
     3061 atccacatgg tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc gtaactcatc
     3121 cccatctgat cgaccatttc cattggtgta cccaggtcgc gcaagatctg ctgccctgcc
     3181 cccagcgctg gcgtattggt taccgcaccg gagaaaatcc ccagcactac cggcagtgga
     3241 atatcaaaca gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca
     3301 gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc
     3361 tgaatcccga tggtataaac gaacaggatc aggccaaact cctgaataac atgcagcata
     3421 tcgctactta atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccaaac
     3481 agcacgccgc caatacctaa tcctatgccg cgaaatttga cgttgccgat aaacaaaccg
     3541 acgactgcca ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc
     3601 tcttgagata acattttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct
     3661 tatagcgcac attatcacgg gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa
     3721 cgcattgttg aagaagaaag acaaaaaaag ccccaccaga atggcggggc aaagagaata
     3781 gctagttagc tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt
     3841 tcaggctcat tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag
     3901 acttccatat tttcagccag cgtaaaactc aggctaaatg gctgattcat aagcccttga
     3961 tgcagccatt ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgta
     4021 ccttccagtt gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg
     4081 tagtggttat cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg
     4141 ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa
     4201 tcgaagttac gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa
     4261 gattcgcata tgctccctga cggcgagcat ggagatgtca ggccgcgcca ggcggcctta
     4321 gggaattagt tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga
     4381 tcgatataca gcaaaccatt taccaggtta gccccacgaa catgaatgtt ctcagctaac
     4441 tggaatttgc gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt
     4501 tgttcgtcgg cgtgagcacc tttcaccacc agcagattat cctgggcggt aatttccagt
     4561 tcgctctcag caaaaccagc cacagcgata gcaatgcggt aatggttttc gtctaccagt
     4621 tcaacgttat acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta
     4681 aacaaacggt caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata
     4741 atcaatagct cctgaaatca gcgagaatgt aagaccttcc acaatggaca ggtcaggtag
     4801 ccagaacacc catcaggcgc gtcctcatcg actacgatgt aaaatgggtc tggaaatgaa
     4861 cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag
     4921 ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat gatgatgtta
     4981 ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt
     5041 attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatgac attactcgga
     5101 ggatgctcca gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc cggcacccgc
     5161 gccagcgcca ccatgattgg cgacgatgaa accaactggg gcactaagtc gctggcgatt
     5221 ctcgatatgc cgtttaccgc tgtaatggac acgcttttac tgccgtggga tgtgttccgc
     5281 aaagacagct cggtgcgctc gcgcgttgaa aaaagcgaag ccaacgcgca agccaccaat
     5341 gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc
     5401 acagctggcc accttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt
     5461 ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgccg ctttgcgcat
     5521 gggcacaaac aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt
     5581 taaatgcaga ctgaatatcc gttttgttat gggttaactt gcgcggttcg ccgccctgtg
     5641 gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg
     5701 taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc
     5761 gatgatgggt aaaggttaaa cgtcgctgca cgacgccacg cggtggcgcg ggtagggttg
     5821 tttccgttcc acttaacggc gcatcacctg ccgatttcca gctagcttca tcttgcggta
     5881 actcaacgat aaatagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa
     5941 acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt tcatcactgc
     6001 caggctgcgg cgtgggcgtg gttttactca ccagcacaca ccagtggcta ccgctgtatt
     6061 cacgcggatg ttgcttttgt acgttgaccg ggccaaacgg tgcagcaacc ccgacgtttc
     6121 gcaaatccag cgccggatcg agttgatgca ttacatggtc gttataggta aagctccccc
     6181 tttcaccgtt cgggctaaag acatgcacat ggctgccacc gcgcagcgct cctggcgtat
     6241 acggcgcggt aatatccatt gcatcgagat tgctcacctt gccgccttca gcaatgactc
     6301 cgcgtcgatg atggaagtcg taatgccatg tttcatcagg attttcaggg ccgtgaataa
     6361 agacatattt ctctgacttc ggatgaacgg tcaccacgcc gacgtgtgcg ccctgtgacg
     6421 cgcgatagat aacctcgacc tcgcctgtat ggatattcac acgctcaatg gtctcgccgg
     6481 taaacgacgc cccagaagga cgcacgtcaa ataccagcca ctggctgtcg ggcgtccagg
     6541 tattggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc tgtttcatgg
     6601 cgcggtcctg atacaaaacg gttcccgcca tcatacttca caaggacttc actttcagac
     6661 aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc gacgtggcga
     6721 ttattggcct cggcccggca gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg
     6781 tgatcgcgct ggataaaaag caccagtgcg gtactgaagg tttcagcaaa ccttgcggcg
     6841 gtctgctggc accggacgcg cagcgatctt ttattcgcga tggactgacg cttcctgtcg
     6901 atgtgatcgc caatccacag attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca
     6961 cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac ttgtggatga
     7021 aatcgctgat ccccgccagc gttgaggttt atcacgatag cctgtgccga aaaatctggc
     7081 gtgaggatga taaatggcat gtcatttttc gtgcagacgg ctgggagcaa catattaccg
     7141 cccgctatct ggtcggtgcc gatggcgcaa actcgatggt gcggcgacat ctctacccgg
     7201 atcatcaaat ccgtaaatat gtcgctatcc agcagtggtt cgcagagaaa catccggtgc
     7261 cgttctactc ctgcatcttt gataatgcga taactgactg ttactcatgg agtatcagca
     7321 aagatggtta ttttatcttt ggcggtgcct atccaatgaa agacggtcag acgcgtttca
     7381 cgacgctgaa agagaaaatg agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg
     7441 agaaatgcac ggtgttgttt ccctcacgct ggcaggattt tgtctgtggt aaggacaacg
     7501 cctttctgat tggtgaagcg gcgggattta tcagcgccag ctcgctggag gggattagct
     7561 atgcgctgga tagcgcagag attctgcgtg cggtgttcct gaaacagccg gagaagagca
     7621 acgccgctta ctggcgcgcc acacacaaac tgcgtttaaa actcttcggc aagatagtaa
     7681 aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc
     7741 atattccaca gttgaaagat tatccaacgc gcttcacatc acccaccagc aggatgtaag
     7801 agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggcgcg aaaccgtaac
     7861 cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca ccggcgaagt
     7921 taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagag accagcgacc
     7981 aggtaatcga agcaaaaccg ttgccgaaga atgccagcgc catcaggcac ataatcatca
     8041 tcggatcgtt ggtgtagtta gcgcccataa tgcaggtgga gatcagcaag ccgcagataa
     8101 tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga tccgcgaccc
     8161 agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc ataaagcctg
     8221 ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa
     8281 accacagtgc agaagccacc gcaaattgcc caagataaac gccgatcagt ttacgatgga
     8341 acaccagttt ccagtctttg gctgttaacg gctgacgcgc ctctttcttc accggcgcat
     8401 caccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg ctgatacctt
     8461 tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata atgccgatac
     8521 caccagtgac aatgaacacc cagtgccagc tcaacatctc ctgaatccag atcagcagcg
     8581 gagtcaaaaa cgccagaccg acaaactgac cagacgtata aaaaccaacg gcggaagcgc
     8641 gttcatgttc cgggaaccag ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt
     8701 cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat taatcccgtg gcaaagcctt
     8761 ggaataaagt caccactgac caaccaaata tcgcaataaa ataagtcacg cgagaaccta
     8821 cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt ataaagccag gcgaaggccg
     8881 aaaatacata gcccatttcc gctttcgtaa tgccgaactc ttcctgaata tgggcggaag
     8941 ccacggccaa gttagcgcgg tcaacataac aaatgaccac cgtaataaag atcatcacca
     9001 gcgtcagata acgccgacgc cccggctttg ctgcattaac gggaatatcc atagcgagct
     9061 ttctccagat tttgggcata gcgaagccgc tcaccatgcc ctgtaattta cagagggtta
     9121 tatttttgta ttgctgtttt agtgcccgat gaggggctta cgtggcagga attaccactc
     9181 tgctacgctg ttatcttcat gacgccagag cggattacgc cagtccgggg catttttact
     9241 gaactcaata actttagctt cgtcgatttc cacacctaag cccggtttcg ttaacggttt
     9301 aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc acaaagtcga gtaactccgc
     9361 gcctttgttg taatgaatgc ccatactttg ttactgaagt accgcgttat aggaaacaaa
     9421 gtcgatatgc aggcaagccg ccagtgcaat cggtccgaac ggacagtgcg gcgcaagggt
     9481 cacatcatag gcttctgcca ttccagcaat tttgtagcat tcggtaatac cgcctgcatg
     9541 ggagagatcc ggttgcagaa tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa
     9601 acgtgagaac atgcgctcac ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg
     9661 gtagtattcg gcttgttccg ccagcaccgg ctcttcaata aacagcgggc gatacggctc
     9721 cagttcttta atcagcactt tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc
     9781 aaactcaatc tgattgccaa aagcttcacg aatttgtgcc acggtgttga ctgccgcatc
     9841 taccgcgcgg gagttatcaa ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc
     9901 gaagccgatt tcgcgcaggg ttttaatgcc gtcgataaca tccgccggac gatcgccgcc
     9961 gacccaactg taggctttaa ttttgtcgcg aaccaggccg cccatcagtt gccagactgg
    10021 cgcattcagc actttgcctt tgatatccca taacgcctgg tcgatcccgg cgatggcgct
    10081 catcaggatt ggaccgccac gataaaatcc ggcgcgatac atcacttgcc ataagtcgtt
    10141 gatgcgcgaa ggatcctgac caatcaaata gtcacccagc tcgtgaactg ccgcttccac
    10201 cgtacgggcg cggccttcaa tcacgggctc gccccaaccg accacgcctt catcggtttc
    10261 aattttcagg aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat
    10321 tgcactgcct ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg
    10381 gactgcccgg cgcgataaag atcgctgcct aagcccgccc caacacaacc tgcgtctatc
    10441 cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccggtggt
    10501 aataccgctt ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg aaatattttc
    10561 agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg
    10621 caaacggtca tgccgtagcc caccgcgcgg cggatcactt cactatggat attgggcgta
    10681 acgatgagct gacagcccat cctggcgagc gcatcgacct gttcaggttt cagtaccgta
    10741 cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctctgctcc
    10801 cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca
    10861 acatgcgcca gcgcctcgtc gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta
    10921 gtttgccact gcatgagcga tgctccttat accagcctga aatgccgtgt cgcccgccac
    10981 cgccgccacg tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt
    11041 tccggcgaca agggtgatgg cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc
    11101 tgcgccaatc aacaaaccag agagaaattc gctgacctgt tcgcggggaa gtgttcccag
    11161 cacatgcgag gcgcgaactt caaaaagctg cggcaatatg gcgggcgcat taaggccgcg
    11221 ctcaaggcca gccgcgaagg catcggcaga gttttcctgc ggcggcaaac ctgcgccaat
    11281 caatgagtga tttaacagta aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt
    11341 gatttgctgg ctatcggcct gcacccattt gcaatgcgta ccgggcataa cataaagaga
    11401 ggaaggagcc agagctcgcg cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg
    11461 gttatcgtca tgagagacgc ataatccggg aataatccag atattgtcgc caacagacgt
    11521 taattgttcg ccaatagacg aaaaacgggc aggaacagat aaatacggag caactttcca
    11581 gccgacattg ctgccgacca ttcctgccat caccactggc gtattttctt cacgccagtc
    11641 ggtcgtgact tctgctaaca ccacagccgg agattttccg ttcaggcgcg tgacgcctgc
    11701 ttctgattgc ctgctctcca ggcagtggtc gccctgataa agccaggcgc gcagattggt
    11761 cgatccccag tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc
    11821 gagctggcaa tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc
    11881 gcatcgaaca gcgccttatg ttcctggagc gtttgcggca tgttggcctc atcgcccatc
    11941 caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg caatgctaag ttgctgtaac
    12001 accgggttat gcaccgactg cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac
    12061 gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga
    12121 tcgctggagg tcgcgcgctc tgctgcccaa cgggcaatcg ccggttccac cagatttcgc
    12181 acttcgctca tggcactgat aagccgtggg tcgtagtcgt tttccagcac ccattgcagt
    12241 acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca caaacgcccc gcgataacgt
    12301 ttcatttcaa tcagccgctt cgccatcagc gaacggaaca cctcacggat gatgttgcgc
    12361 gaggttgcaa actcttcaca gagctccgcc tcagccggaa gtggcgagcc aggtacgtat
    12421 ttgccgtgaa cgatctgttt acccagcgta atgacaatgc gatcggtttt attgagagtc
    12481 atggagagtc cttgtgctct gtatgttctt ctctacttta ccccgatcga tgcataacgc
    12541 ggcaactttg tagtaccagc gtgatgacgt tcgcgtttgc cgcgcgcgta atgtagtaca
    12601 aacttatatt gttatgctac aatttagatc aaaaaaagaa caatacataa aaaaatgaca
    12661 tgcgtcgggc agaaatctga aaagggatat caggcgctaa acaggaggga aagaagagta
    12721 tgctttcaac ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga
    12781 ttttttaagg ctgccggttt attcccactg gcgctgatgc tttcaggctg tatctcgtat
    12841 gctctggttt cccataccgc aaagggtagt tcaggaaagt atcaatcgca gtcagacacc
    12901 atcactgggc tatcgcaggc aaaagatagt aatggaacaa aaggctatgt ttttgtaggg
    12961 gaatcgctgg attaccttat cactgatggt gccgatgaca tcgttaagat gctcaatgat
    13021 ccagcactta accggcacaa tattcaggtt gccgatgacg caagatttgt tttaaatgcg
    13081 gggaaaaaga aatttaccgg cacaatatcg ctttactact actggaataa cgaagaagaa
    13141 aaggcactgg caacgcatta tggttttgcc tgtggtgttc aacactgtac caggtcactg
    13201 gaaaacctaa aaggcacaat ccatgagaaa aataaaaaca tggattactc aaaggtgatg
    13261 gcgttctacc atccatttaa agtgcgattt tatgaatact attcacccag aggcattccg
    13321 gatggtgttt ccgcagcatt actgccagtg actgttacac tggacatcat tactgcaccg
    13381 ctgcaatttc tggttgtata tgcagtaaac caataatcag taagcgggca aacgcgttta
    13441 tgctgtttgc ccgcttacag attaattcag cacatacttc tcaatagcaa acgccacgcc
    13501 atcttcaagg ttggattttg tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt
    13561 atccatcgcc acaccgacgc ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc
    13621 aatcgccatg atttcttccg gtttaatacc taacacgtcg gccagtgatt tcacacccgt
    13681 acctttgtta acgcgtttat cgaggatttc gaggaagtac ggcgcacttt tcagcacggt
    13741 atatttctct ttcacttcct gcggaatacg cgcgatagcc tggtcgagga tggcgggttc
    13801 atcaatcatc atcactttca ggaactgggt attggggtcc attttctccg cttcgcagaa
    13861 caccagcgga attgtggcaa cgaaggattc atgcaccgtg tagtagctga tatcgcggtt
    13921 agcggtgtac agcgtggtgc ggtccagggc gtggaaatga gaaccgactt cgcgagagag
    13981 tttttccagg aaacgatagt cgtcatagct gagagcggtt tgcgccacgg tgctaccatc
    14041 agcggccttc tgtaccagcg cgccgttata agtaatgcag tagtcgcccg gctgttccat
    14101 atgcagctct ttcaggtagt tgtgcacacc tgcatacgga cgacccgtcg ttagcacgac
    14161 attcacgcca cgggcgcgag ctgcggcaat cgcattttta acggcgggtg aaatggtgtg
    14221 atcgggcagc agaagggtgc catccatatc gatagcaatg agtttgatag ccatgagttc
    14281 ccaaggtaga ttagttcctg acccatgcta acgcgattcc gctcaaaaat cagtacaaca
    14341 cccgagggaa aaggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
    14401 ttccctttca gcagttccat gcctgcggaa agcagatcgt tattggcttg tgctgacact
    14461 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gctctgccag taaactggaa
    14521 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc
    14581 gccgactcca gttgctcgcc gctaactgat tgatttctct gttgattact caaccaggtt
    14641 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccaccc
    14701 tgctcctcaa cccaacttaa aatcgcctga tatttccccg catcgccttt cagaaaggca
    14761 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
    14821 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
    14881 tctccgccct tttcttatag cttcttgccg gatgcggcgt gaacgcctta tccggcctac
    14941 aaaatcatgc aaaatcaata tattgcagga gtctcgtagg actaataagc gtagcgcatc
    15001 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
    15061 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaactggt
    15121 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acggctttcc gggtccatgg
    15181 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
    15241 ggccacgacg ggactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
    15301 gacgacgctc gccacgttcg ataaacgcat cttcttccag caagccacgc agtttctcac
    15361 ccagcgtgca gatacgacga tattcgccac cggtgataaa ctcgtgatcc agcggatagt
    15421 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
    15481 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
    15541 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga aaggtcagct tccgtcaacg
    15601 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
    15661 tcattttctg cgtcgcgttg tactcagata ccagtttctc taacgcttcg ccagccaatg
    15721 ccggtgcact ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
    15781 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
    15841 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
    15901 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
    15961 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
    16021 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
    16081 cgacgttaag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
    16141 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
    16201 cgcgttcctg gcagtctgcc agtttgcccg gcaggccagc taagtcgagc gcacctttac
    16261 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
    16321 tgccaaccac gattttcgca tcagttgggt tttccagcag gtattccgcc agcagttcgt
    16381 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
    16441 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
    16501 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcat
    16561 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
    16621 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcagcgcca
    16681 cttcaacgcc aataccgtct ttttcggtgg agaagtagaa gatatttggg tggatcggcg
    16741 ttttgttctt gttcagatat tcaacgaacg ctttgatgcc gccttcatag tggaagtggt
    16801 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
    16861 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
    16921 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
    16981 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgaa
    17041 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
    17101 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
    17161 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
    17221 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
    17281 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
    17341 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
    17401 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
    17461 tacttgggtt aatctgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
    17521 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
    17581 tttaagcggc tggcaagcaa cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
    17641 atcaggtaga gacaccgccg cccgctttca cgagtgagga actctccttg cgccagacgt
    17701 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
    17761 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
    17821 tcgcgttcaa aattacgttc cagcacctca gcgtactctg tctctttctc ccagccgcgc
    17881 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaggtatc atccatatcg
    17941 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccgc cagcgggatc
    18001 agttctttat cccacgggcg tagctgttcg taacgagtca cctggcgcag cgccgcattg
    18061 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccagg ttcgttgtga
    18121 aagcatcccc agtcgaggaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
    18181 ccttctggcg ttatcaactg catcggcatc aggtgcgcca gttccgcgac tttatgcccg
    18241 tccgtaccgt caatacggac tttgctgtcg ccctgtttgt ctttggttaa gccaatcgcc
    18301 gtctcgcgct cttcgccctg taatcgcccg tggagaacaa atgcctcctg ctcatggcga
    18361 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
    18421 tccagcacgc tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
    18481 aaagcgagat ccgcggtttc aatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
    18541 attacagtct cattggcatg acaacatagg cagccgactg tgatgcggca tcttcaatct
    18601 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
    18661 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
    18721 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtaatt ttcagctggt
    18781 tttcgctgac atacaggcgc acaccgcgga atttctcgtt agagagaatc gccgcgcgag
    18841 caaacgcctg cttgagcaga tcgcagcctg cttccagatg tttgtccggg ttcttcggca
    18901 gaacgcgacg gtaatccggg aagcgaccat ccaccagttt cgaggtgaaa ataaagtcgc
    18961 caacgtgggc acgaatgtta ttgctgccaa tctgcacacg cagcagattg tcgccgccgt
    19021 cgagcatacg catcagttca atcacgcctt tacgcggtac gatcaccgaa tggcttggca
    19081 aagattgacc aattggcatt gaacagaccg ccagacggtg gccgtcggtt gccacggtgc
    19141 gcagttcttc accttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
    19201 gcgccatcga aaactgggtt gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
    19261 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
    19321 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
    19381 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
    19441 tcgctcctgg ctcgtgtggc tgaaccagcg caacgcgtgc caccatttcc atctcgagat
    19501 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
    19561 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
    19621 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
    19681 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
    19741 accgtcgtgt ggtcacgccc accaaacgca tcgccaatct ctggcagact gtggttagtc
    19801 agctctttcg ctagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
    19861 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
    19921 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
    19981 tcgatggtga tcgcccgtcc ggtaaagttg gcgttagcaa taacgcggtt cagcgcccct
    20041 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcacccggc
    20101 aaacgaatgt cattttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
    20161 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
    20221 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
    20281 agggcgttga aggtgtggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
    20341 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
    20401 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
    20461 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
    20521 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
    20581 acctggcaag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
    20641 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
    20701 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
    20761 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
    20821 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
    20881 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
    20941 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
    21001 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
    21061 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
    21121 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
    21181 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
    21241 tctcacagat tttccctatt tatccacagg actttccaga actcgctaag tgtaaacgat
    21301 cctgccgcga ggcgggcacg atttacgccg catattggaa aatttaatga ccatagacaa
    21361 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
    21421 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
    21481 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggtgtgt
    21541 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
    21601 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
    21661 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
    21721 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
    21781 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
    21841 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
    21901 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
    21961 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgcctgacg
    22021 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
    22081 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
    22141 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
    22201 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
    22261 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
    22321 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
    22381 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
    22441 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
    22501 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
    22561 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
    22621 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
    22681 gccgttccag ctgttggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
    22741 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
    22801 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
    22861 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
    22921 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcct cgtttggtca
    22981 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
    23041 gcacaccttc cgtggcgcgg cgtactccac gcctgacgag aagtatgaga aatacaagtt
    23101 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcgat
    23161 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
    23221 taccgctaat ctgggtaacg gcatcgtcgc tatcggctat aaatctcagc cggtactggt
    23281 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
    23341 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
    23401 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
    23461 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc
    23521 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
    23581 gcgtctgggc gatgacaaac agcgtatcag tcaggaaatg atggcgctgt acaaagctga
    23641 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
    23701 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
    23761 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
    23821 gttcttcatt cagaagatgt cgccgaccac tgtgaccgac ccgatgcagc agaagatcat
    23881 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtact
    23941 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    24001 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    24061 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    24121 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    24181 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    24241 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    24301 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    24361 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    24421 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    24481 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    24541 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    24601 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    24661 agcggcaatt gatttccctg atgaagagat cgatttcctc tccgacggca aaattgaagc
    24721 ccagcttaat gacgtgatgg ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag
    24781 tttgttgcgc gaagggatga aagtagtgat tgccggacgt cctaatgccg gtaaatcgag
    24841 cctgttaaac gcgctggcgg gccgtgaagc ggcaatcgta accgatatcg ccggaaccac
    24901 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgacac
    24961 tgccgggcta cgtgaggcca gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca
    25021 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacga cagacgccgt
    25081 tgatccggca gagatctggc cggaatttat cgcccgtctg cccgcgaaac tgccgatcac
    25141 cgtggtacgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    25201 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    25261 tctcaaacag agcatgggct ttgacaccaa tatggaaggc ggcttcctgg cgcgccgtcg
    25321 ccacttacag gcgctggaac aggcagcgga gcatttgcag caaggtaaag cgcaactgtt
    25381 gggagcctgg gcaggtgaac tgctggcgga agagttgcgc ctggcacagc agaacttaag
    25441 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    25501 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    25561 attgttagca ctcctgttat gcctcaaccc ttttttaaac attaaaattc ttacgtaatt
    25621 tataatcttt aaaaaaagca tttaatactg ctccccgaac gattgtgatt cgattcacat
    25681 ttaaacaatt tcagaataga caaaa
//
LOCUS       DADLDT020000058        24600 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000058 DADLDT020000000
VERSION     DADLDT020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.058"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..374)
                     /locus_tag="JQH22_002243"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="JQH22_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="JQH22_002244"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="JQH22_002244"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFB2206544.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="JQH22_002245"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="JQH22_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFB2206545.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="JQH22_002246"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="JQH22_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFB2206546.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="JQH22_002247"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="JQH22_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFB2206547.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="JQH22_002248"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="JQH22_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFB2206548.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="JQH22_002249"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="JQH22_002249"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFB2206549.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="JQH22_002250"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="JQH22_002250"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFB2206550.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="JQH22_002251"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="JQH22_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFB2206551.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="JQH22_002252"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="JQH22_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFB2206552.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="JQH22_002253"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="JQH22_002253"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFB2206553.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="JQH22_002254"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="JQH22_002254"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFB2206554.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="JQH22_002255"
     CDS             10736..11161
                     /locus_tag="JQH22_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFB2206555.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="JQH22_002256"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="JQH22_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFB2206556.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="JQH22_002257"
     CDS             12185..13621
                     /locus_tag="JQH22_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HFB2206557.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="JQH22_002258"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="JQH22_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFB2206558.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="JQH22_002259"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="JQH22_002259"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFB2206559.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="JQH22_002260"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="JQH22_002260"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFB2206560.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="JQH22_002261"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="JQH22_002261"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFB2206561.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="JQH22_002262"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="JQH22_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFB2206562.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="JQH22_002263"
     CDS             complement(20454..20747)
                     /locus_tag="JQH22_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFB2206563.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="JQH22_002264"
     CDS             complement(20744..22033)
                     /locus_tag="JQH22_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFB2206564.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="JQH22_002265"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="JQH22_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="JQH22_002266"
     CDS             complement(23047..23811)
                     /locus_tag="JQH22_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFB2206565.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="JQH22_002267"
     CDS             24127..>24600
                     /locus_tag="JQH22_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFB2206566.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6094 a         5940 c         6465 g         6101 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cgggggggtc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DADLDT020000059        24562 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000059 DADLDT020000000
VERSION     DADLDT020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24562
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.059"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(2..94)
                     /locus_tag="JQH22_002268"
     tRNA            complement(2..94)
                     /locus_tag="JQH22_002268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JQH22_002269"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JQH22_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFB2206567.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JQH22_002270"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JQH22_002270"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFB2206568.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JQH22_002271"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JQH22_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFB2206569.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JQH22_002272"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JQH22_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFB2206570.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JQH22_002273"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JQH22_002273"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFB2206571.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JQH22_002274"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JQH22_002274"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFB2206572.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="JQH22_002275"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="JQH22_002275"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JQH22_002276"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JQH22_002276"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFB2206573.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="JQH22_002277"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="JQH22_002277"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFB2206574.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="JQH22_002278"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="JQH22_002278"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFB2206575.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="JQH22_002279"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="JQH22_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="JQH22_002280"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="JQH22_002280"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFB2206576.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="JQH22_002281"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="JQH22_002281"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFB2206577.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JQH22_002282"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JQH22_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFB2206578.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="JQH22_002283"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="JQH22_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFB2206579.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="JQH22_002284"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="JQH22_002284"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFB2206580.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JQH22_002285"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JQH22_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFB2206581.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JQH22_002286"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JQH22_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFB2206582.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="JQH22_002287"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="JQH22_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFB2206583.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="JQH22_002288"
     CDS             complement(20900..21019)
                     /locus_tag="JQH22_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HFB2206584.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="JQH22_002289"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="JQH22_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFB2206585.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="JQH22_002290"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="JQH22_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFB2206586.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24562)
                     /locus_tag="JQH22_002291"
     CDS             complement(23682..>24562)
                     /locus_tag="JQH22_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFB2206587.1"
                     /translation="LIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGG
                     IGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAV
                     GLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAA
                     PWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAA
                     WVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMA
                     LVELAVLVVIIVSRGA"
BASE COUNT         5796 a         6911 c         6296 g         5559 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cg
//
LOCUS       DADLDT020000060        24183 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000060 DADLDT020000000
VERSION     DADLDT020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.060"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(25..597)
                     /locus_tag="JQH22_002292"
     CDS             complement(25..597)
                     /locus_tag="JQH22_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFB2206588.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="JQH22_002293"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="JQH22_002293"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFB2206589.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JQH22_002294"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JQH22_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFB2206590.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JQH22_002295"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JQH22_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFB2206591.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="JQH22_002296"
     CDS             complement(3559..5340)
                     /locus_tag="JQH22_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFB2206592.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="JQH22_002297"
     CDS             complement(5333..7105)
                     /locus_tag="JQH22_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFB2206593.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="JQH22_002298"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="JQH22_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFB2206594.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JQH22_002299"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JQH22_002299"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFB2206595.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="JQH22_002300"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="JQH22_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="JQH22_002301"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="JQH22_002301"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFB2206596.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="JQH22_002302"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="JQH22_002302"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFB2206597.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="JQH22_002303"
     CDS             complement(11660..12031)
                     /locus_tag="JQH22_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFB2206598.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="JQH22_002304"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="JQH22_002304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFB2206599.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="JQH22_002305"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="JQH22_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HFB2206600.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="JQH22_002306"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="JQH22_002306"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFB2206601.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="JQH22_002307"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="JQH22_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFB2206602.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="JQH22_002308"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="JQH22_002308"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFB2206603.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="JQH22_002309"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="JQH22_002309"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFB2206604.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="JQH22_002310"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="JQH22_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFB2206605.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="JQH22_002311"
     CDS             21802..22380
                     /locus_tag="JQH22_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFB2206606.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="JQH22_002312"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="JQH22_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFB2206607.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6666 c         5828 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 tta
//
LOCUS       DADLDT020000061        23882 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000061 DADLDT020000000
VERSION     DADLDT020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23882
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.061"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(2..298)
                     /locus_tag="JQH22_002313"
     CDS             complement(2..298)
                     /locus_tag="JQH22_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFB2206608.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="JQH22_002314"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="JQH22_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFB2206609.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JQH22_002315"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JQH22_002315"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFB2206610.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="JQH22_002316"
     CDS             complement(1784..2323)
                     /locus_tag="JQH22_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFB2206611.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="JQH22_002317"
     CDS             complement(2353..3096)
                     /locus_tag="JQH22_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HFB2206612.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="JQH22_002318"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="JQH22_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="JQH22_002319"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="JQH22_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFB2206613.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="JQH22_002320"
     CDS             complement(4151..4657)
                     /locus_tag="JQH22_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFB2206614.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="JQH22_002321"
     CDS             complement(4703..5203)
                     /locus_tag="JQH22_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFB2206615.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="JQH22_002322"
     CDS             complement(5289..5468)
                     /locus_tag="JQH22_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFB2206616.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="JQH22_002323"
     CDS             complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="JQH22_002323"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFB2206617.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="JQH22_002324"
     CDS             complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="JQH22_002324"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFB2206618.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="JQH22_002325"
     CDS             complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="JQH22_002325"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFB2206619.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="JQH22_002326"
     CDS             complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="JQH22_002326"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFB2206620.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="JQH22_002327"
     CDS             complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="JQH22_002327"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFB2206621.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12401..12494)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="JQH22_002328"
     CDS             complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="JQH22_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFB2206622.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12653..13534
                     /gene="yciV"
                     /locus_tag="JQH22_002329"
     CDS             12653..13534
                     /gene="yciV"
                     /locus_tag="JQH22_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HFB2206623.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13531..14151
                     /locus_tag="JQH22_002330"
     CDS             13531..14151
                     /locus_tag="JQH22_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HFB2206624.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14252..15124
                     /gene="rluB"
                     /locus_tag="JQH22_002331"
     CDS             14252..15124
                     /gene="rluB"
                     /locus_tag="JQH22_002331"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFB2206625.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="JQH22_002332"
     CDS             complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="JQH22_002332"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFB2206626.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15751..16509)
                     /locus_tag="JQH22_002333"
     CDS             complement(15751..16509)
                     /locus_tag="JQH22_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFB2206627.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16729..17778
                     /gene="sohB"
                     /locus_tag="JQH22_002334"
     CDS             16729..17778
                     /gene="sohB"
                     /locus_tag="JQH22_002334"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFB2206628.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17814..18065)
                     /locus_tag="JQH22_002335"
     CDS             complement(17814..18065)
                     /locus_tag="JQH22_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFB2206629.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18445..21042
                     /gene="topA"
                     /locus_tag="JQH22_002336"
     CDS             18445..21042
                     /gene="topA"
                     /locus_tag="JQH22_002336"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFB2206630.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21252..22226
                     /gene="cysB"
                     /locus_tag="JQH22_002337"
     CDS             21252..22226
                     /gene="cysB"
                     /locus_tag="JQH22_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFB2206631.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22521..22685
                     /locus_tag="JQH22_002338"
     CDS             22521..22685
                     /locus_tag="JQH22_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFB2206632.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22688..22855
                     /locus_tag="JQH22_002339"
     CDS             22688..22855
                     /locus_tag="JQH22_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206633.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22969..23064
                     /locus_tag="JQH22_002340"
     CDS             22969..23064
                     /locus_tag="JQH22_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206634.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23228..>23882
                     /locus_tag="JQH22_002341"
     CDS             23228..>23882
                     /locus_tag="JQH22_002341"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFB2206635.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT         6017 a         5889 c         6089 g         5887 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccga agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccatac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgtc accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaacc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaaat atattttgag gaacattctg gatatattaa caattacctg aggaatatgt
     5641 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     5701 tattactgtt gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt
     5761 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     5821 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     5881 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     5941 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     6001 gatccggggc ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct
     6061 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc
     6121 gtgacagcaa atatgtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     6181 cggcatttgg cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga
     6241 agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct
     6301 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     6361 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     6421 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg
     6481 ccagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     6541 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     6601 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     6661 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     6721 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcagcgcatg
     6781 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     6841 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     6901 aagatacgcg tgttgtggac cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     6961 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aataaatgcc
     7021 cacgcgacca tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccaccagg
     7081 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     7141 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     7201 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacggacaa tggtcggata
     7261 aggatgcggg ccagccgcgg tgcccagcat atagtgcgcg gtttcataac tgccggacca
     7321 gtcgcgtagc gcctcattac aggcatcttt cagcgtggcg gaaccgctat gtaccgggat
     7381 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt
     7441 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     7501 gccatgctga ccggccccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     7561 caacgcctgc cccagcacct ggttggtttt atgtgcgccg ccgtgcagca aatcttcacg
     7621 cttgagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagtgcggt
     7681 tggacgcccg gcatagtttt tcagcaggtc gttgaactgg gcttgaaatt caggatcttt
     7741 ttgcgcgctg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     7801 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     7861 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt
     7921 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     7981 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     8041 agtgaccagt caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     8101 acgtgctgaa actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     8161 acatgtgctg gcagagcttc acgcagcgta tcgatataca gctgatcttc attaccatgc
     8221 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     8281 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     8341 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     8401 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     8461 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     8521 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     8581 ctgattaccg ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     8641 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc taatgcaatg
     8701 gcgcgctcca gttcctcttc attactgact tcggtcagca cccccatctc cagactgtga
     8761 gcgacggctg caagctggcg atattggtcg tcatccagta ctgaaagcat taataagcag
     8821 gcatcggcct ggtaatagcg cgccagatag atctggtaag ggtcgataat gaagtcttta
     8881 cataaaatcg gctgcggggc gatttggctg acgatgagga ggaaatcaaa gctcccctga
     8941 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     9001 atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttacac
     9061 tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg tgtgctcggc
     9121 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     9181 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagc
     9241 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     9301 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     9361 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     9421 tcaggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     9481 gtcaattgat agctcttaat tttgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     9541 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     9601 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     9661 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     9721 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat atttcggcgc aaagaggaaa
     9781 catacgccta actcgtccag cgcctggcgc gatttattgg cgttcatatc aagattaata
     9841 ccgaatgccg ccagcagatc cgacgagccg gatttactgg agacgctgcg gttaccgtgt
     9901 ttcgccactt tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata
     9961 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
    10021 aacggcgctg cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca
    10081 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
    10141 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
    10201 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
    10261 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
    10321 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
    10381 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
    10441 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
    10501 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
    10561 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
    10621 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
    10681 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta
    10741 tgaccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
    10801 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
    10861 gtacagcgcg ggctttgtta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga
    10921 ctacaccagc acccgcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
    10981 caatgcaggt gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc
    11041 cgcggcgacg accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccgc
    11101 ttaacgtccc catattcata caggcgcgat aagcgtgcag ggcgtcaaga tcgtgacgca
    11161 gttcgccgac tacgcgagag acgaggtgca tcacatagga ataacggtca actttggtga
    11221 gatcggcgat gtagcggctg ccaggggtgc aaatgcgtgc cagatcatta cgggcgagat
    11281 caaccagcat cagatgttca gacagctctt tatggtcagt acgcatttcc agttcaatac
    11341 ggctgtcgag atctctgtcc agtgaaccat cggcgcgacg accgcgtggg cgtgttccgg
    11401 caatcgggta gatctcaatc tggcggctgg tggcatcata cttgagcgag ctttccggcg
    11461 acgcgccaaa tagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
    11521 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
    11581 atggcaccac ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc
    11641 caccgaactc ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccactg
    11701 gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac
    11761 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
    11821 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
    11881 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
    11941 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga
    12001 aagcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag
    12061 gggggaagcg cagcacacgg cagtttggtg attgttcact ttccacaccc gcaggcaggg
    12121 cgttatccag tagtgccagc agggcttcgc cattgccgga aagtgcctga attgtgacgg
    12181 tgtcacctaa tgctgtaatg cgcagcgcac tgtctatcag cagcaggctt tttaaatcat
    12241 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgattc ccacacaact
    12301 ggtgaaaaag cgcagtcggg ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
    12361 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
    12421 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
    12481 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
    12541 tgcgcactag ttaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    12601 agacccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    12661 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    12721 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
    12781 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
    12841 ggcgctgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
    12901 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
    12961 gacagaacgg cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat
    13021 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
    13081 ttttgcgcgt ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa
    13141 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
    13201 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    13261 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc
    13321 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc
    13381 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    13441 cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga
    13501 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    13561 aacccgcagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
    13621 gtctatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgccatg
    13681 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    13741 gatctttctg aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa
    13801 aacaacacgc cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt
    13861 ctgttgcagg agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    13921 gcgttgcttg aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    13981 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    14041 attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat
    14101 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    14161 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    14221 gcctgtgaag gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    14281 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    14341 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    14401 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    14461 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    14521 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    14581 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    14641 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    14701 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    14761 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    14821 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    14881 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    14941 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac
    15001 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg
    15061 tgcggtgaaa cgtcacagtc aggtgagcgg cagtcgtcgt tctggcgggc gtaataacgg
    15121 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta
    15181 tttgcgcctt aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat
    15241 ccgccagttc aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat
    15301 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    15361 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    15421 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    15481 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    15541 cattaggcca ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg
    15601 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    15661 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    15721 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    15781 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    15841 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    15901 tgcgcgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg
    15961 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    16021 accccagttg gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    16081 gtccgatttg agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt
    16141 gacctgcatg acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc
    16201 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg
    16261 tgccagttgt tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    16321 ctgacgccca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    16381 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat
    16441 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    16501 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    16561 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    16621 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    16681 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    16741 tctgaatatg gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt
    16801 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    16861 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    16921 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    16981 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    17041 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    17101 acggcggtac tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct
    17161 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    17221 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    17281 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    17341 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    17401 accgccgggc agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg
    17461 gagaaattcc gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt
    17521 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    17581 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    17641 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    17701 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    17761 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    17821 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    17881 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    17941 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    18001 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    18061 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    18121 tataccacgc tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa
    18181 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    18241 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    18301 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagagcg
    18361 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    18421 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    18481 aaagccaaaa cgatcaacaa gtatctgggt tgtgactacg tggtgaaatc cagcgtcggt
    18541 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    18601 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    18661 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    18721 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    18781 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg
    18841 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac
    18901 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    18961 cgcgtggtgg ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct
    19021 gccggtcgtg tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa
    19081 gcgttcgtac cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa
    19141 gcgttggcgt tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa
    19201 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    19261 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    19321 gccagcaccc gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    19381 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    19441 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    19501 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    19561 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    19621 ttaatctggc gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg
    19681 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gtgctttgat
    19741 ggctggacaa aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca
    19801 gtcgataaag gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag
    19861 ccgccagccc gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt
    19921 cgtccgtcta cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta
    19981 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    20041 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    20101 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    20161 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    20221 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagt
    20281 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    20341 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    20401 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    20461 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    20521 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    20581 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    20641 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa
    20701 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    20761 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    20821 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    20881 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    20941 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    21001 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    21061 ttttataaac aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac
    21121 acctatacac taaggctata aatgatatag tggttatagt tagcaccttt tttattatta
    21181 aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
    21241 gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
    21301 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
    21361 tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga
    21421 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
    21481 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
    21541 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
    21601 agcgttatcc ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg
    21661 ccgtctctaa aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
    21721 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
    21781 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
    21841 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
    21901 cgccgcgtat cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
    21961 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
    22021 tgcgcgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
    22081 gtactttttt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
    22141 gtgatgtcgt tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta
    22201 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
    22261 gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta
    22321 tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa
    22381 ttccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc
    22441 gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat
    22501 tacaccaatc attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc
    22561 cttctggaaa tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg
    22621 tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg
    22681 gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa
    22741 tgaaccagaa acgaaacggg aatgttccgt cgttattcca gacgactggc aactaacatc
    22801 gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc
    22861 atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac
    22921 agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca
    22981 aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa
    23041 ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt
    23101 tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt
    23161 tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg
    23221 aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa
    23281 aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct
    23341 acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt
    23401 taccgaagag gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga
    23461 aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt
    23521 tgatctggcg gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa
    23581 cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga
    23641 tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt
    23701 gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg
    23761 catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga
    23821 cggtgaatgg attgcttatc cggatacact cgttggtact gactcgcaca ccactatgat
    23881 ca
//
LOCUS       DADLDT020000062        22556 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000062 DADLDT020000000
VERSION     DADLDT020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22556
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.062"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            187..480
                     /locus_tag="JQH22_002342"
     CDS             187..480
                     /locus_tag="JQH22_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFB2206636.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQH22_002343"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQH22_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFB2206637.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="JQH22_002344"
     CDS             complement(1922..2374)
                     /locus_tag="JQH22_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFB2206638.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="JQH22_002345"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="JQH22_002345"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFB2206639.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="JQH22_002346"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="JQH22_002346"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFB2206640.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQH22_002347"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQH22_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFB2206641.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQH22_002348"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQH22_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFB2206642.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQH22_002349"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQH22_002349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFB2206643.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQH22_002350"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQH22_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFB2206644.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQH22_002351"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQH22_002351"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFB2206645.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQH22_002352"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQH22_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFB2206646.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQH22_002353"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQH22_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFB2206647.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="JQH22_002354"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="JQH22_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFB2206648.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="JQH22_002355"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="JQH22_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFB2206649.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="JQH22_002356"
     CDS             complement(15085..15582)
                     /locus_tag="JQH22_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFB2206650.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="JQH22_002357"
     CDS             complement(15579..15941)
                     /locus_tag="JQH22_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFB2206651.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="JQH22_002358"
     CDS             complement(15931..16278)
                     /locus_tag="JQH22_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFB2206652.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="JQH22_002359"
     CDS             16387..16800
                     /locus_tag="JQH22_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206653.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="JQH22_002360"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="JQH22_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="JQH22_002361"
     CDS             complement(18324..20039)
                     /locus_tag="JQH22_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFB2206654.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="JQH22_002362"
     CDS             20206..21099
                     /locus_tag="JQH22_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFB2206655.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="JQH22_002363"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="JQH22_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..>22556)
                     /locus_tag="JQH22_002364"
     CDS             complement(21910..>22556)
                     /locus_tag="JQH22_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFB2206656.1"
                     /translation="EPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLID
                     QVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLY
                     SKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQTL
                     DDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         5740 a         6110 c         5658 g         5048 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caagaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcgg
//
LOCUS       DADLDT020000063        22392 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000063 DADLDT020000000
VERSION     DADLDT020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22392
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.063"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..434)
                     /locus_tag="JQH22_002365"
     CDS             complement(<1..434)
                     /locus_tag="JQH22_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2206657.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            442..942
                     /gene="tnpA"
                     /locus_tag="JQH22_002366"
     CDS             442..942
                     /gene="tnpA"
                     /locus_tag="JQH22_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFB2206658.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(981..1328)
                     /gene="grxD"
                     /locus_tag="JQH22_002367"
     CDS             complement(981..1328)
                     /gene="grxD"
                     /locus_tag="JQH22_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFB2206659.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1663..2490
                     /gene="mepH"
                     /locus_tag="JQH22_002368"
     CDS             1663..2490
                     /gene="mepH"
                     /locus_tag="JQH22_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFB2206660.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2618..3199
                     /gene="sodB"
                     /locus_tag="JQH22_002369"
     CDS             2618..3199
                     /gene="sodB"
                     /locus_tag="JQH22_002369"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFB2206661.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3345..4514)
                     /locus_tag="JQH22_002370"
     CDS             complement(3345..4514)
                     /locus_tag="JQH22_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206662.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="JQH22_002371"
     CDS             complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="JQH22_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HFB2206663.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5068..6093
                     /gene="purR"
                     /locus_tag="JQH22_002372"
     CDS             5068..6093
                     /gene="purR"
                     /locus_tag="JQH22_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFB2206664.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6090..7022)
                     /locus_tag="JQH22_002373"
     CDS             complement(6090..7022)
                     /locus_tag="JQH22_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2206665.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7135..8346
                     /locus_tag="JQH22_002374"
     CDS             7135..8346
                     /locus_tag="JQH22_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HFB2206666.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8637..9785
                     /gene="cfa"
                     /locus_tag="JQH22_002375"
     CDS             8637..9785
                     /gene="cfa"
                     /locus_tag="JQH22_002375"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFB2206667.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="JQH22_002376"
     CDS             complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="JQH22_002376"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFB2206668.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10681..12054
                     /gene="mdtK"
                     /locus_tag="JQH22_002377"
     CDS             10681..12054
                     /gene="mdtK"
                     /locus_tag="JQH22_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFB2206669.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12095..13351)
                     /locus_tag="JQH22_002378"
     CDS             complement(12095..13351)
                     /locus_tag="JQH22_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFB2206670.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13659..13735
                     /locus_tag="JQH22_002379"
     tRNA            13659..13735
                     /locus_tag="JQH22_002379"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13693..13695,aa:Val,seq:gac)
     gene            13740..13816
                     /locus_tag="JQH22_002380"
     tRNA            13740..13816
                     /locus_tag="JQH22_002380"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13774..13776,aa:Val,seq:gac)
     gene            13924..14229
                     /locus_tag="JQH22_002381"
     CDS             13924..14229
                     /locus_tag="JQH22_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFB2206671.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14355..15959
                     /locus_tag="JQH22_002382"
     CDS             14355..15959
                     /locus_tag="JQH22_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFB2206672.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15971..16783)
                     /locus_tag="JQH22_002383"
     CDS             complement(15971..16783)
                     /locus_tag="JQH22_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206673.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIAGGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16787..17572)
                     /locus_tag="JQH22_002384"
     CDS             complement(16787..17572)
                     /locus_tag="JQH22_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFB2206674.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17569..18237)
                     /locus_tag="JQH22_002385"
     CDS             complement(17569..18237)
                     /locus_tag="JQH22_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFB2206675.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18301..18939)
                     /locus_tag="JQH22_002386"
     CDS             complement(18301..18939)
                     /locus_tag="JQH22_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFB2206676.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18952..21053)
                     /locus_tag="JQH22_002387"
                     /pseudo
     CDS             complement(18952..21053)
                     /locus_tag="JQH22_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(21074..21700)
                     /locus_tag="JQH22_002388"
     CDS             complement(21074..21700)
                     /locus_tag="JQH22_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFB2206677.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(22156..22365)
                     /gene="fumD"
                     /locus_tag="JQH22_002389"
     CDS             complement(22156..22365)
                     /gene="fumD"
                     /locus_tag="JQH22_002389"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFB2206678.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5601 a         5793 c         5201 g         5797 t
ORIGIN      
        1 cccgtgacct gacggctatt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgtcctgc gggtagcgga atgcagcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
      181 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccacctggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      481 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac caaataccga
      541 cgccagattt ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt
      601 gctgattttg cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      661 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcatatcg
      721 aataggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt
      781 ttgtggagtc ctggctattt tgccagtagc tgcgaaggtg cgccaatatc cgtcatccgc
      841 cagtacattg aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc
      901 cctgaaggac gaggttttac gacgcaccgg ataaaggact gacggatgcc cgttcgcatc
      961 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
     1021 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
     1081 ccgccgacca gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
     1141 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
     1201 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
     1261 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
     1321 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
     1381 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
     1441 atttgttgca tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata
     1501 accattttta gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa
     1561 caataaaagc tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc
     1621 agacgcaggc atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt
     1681 atttcgatca cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat
     1741 gcttcaaagc aagccaggga gagttctgct accactcata tcaccaaaaa agcagataaa
     1801 aagaaaagca cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa
     1861 gccgccagta aaagtatgac caaaagcaaa accgcttctt ctgttaaaaa atcttccatt
     1921 accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc
     1981 agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa
     2041 aatgccgcgt caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa
     2101 gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca
     2161 ccgcataccg gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa
     2221 attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa
     2281 cgtagtgagc tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc
     2341 gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag
     2401 gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt
     2461 cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt
     2521 aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta
     2581 ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact
     2641 accatatgct aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta
     2701 cggcaagcat catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt
     2761 tgaaggtaaa tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc
     2821 agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga
     2881 accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa
     2941 agcgcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa
     3001 aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac
     3061 cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg
     3121 caatgcacgt cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc
     3181 gaaaaatctc gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt
     3241 accatgccaa cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc
     3301 agcaactgta acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg
     3361 caaactgttt caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg
     3421 acaatcgccc ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca
     3481 ccagcagctg ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac
     3541 agacctggcg cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca
     3601 aaggttgctg cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg
     3661 aacgggattg ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta
     3721 actgaacgat ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc
     3781 agcaccagca ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga
     3841 gagatatagg tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca
     3901 gacagcacct gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct
     3961 gtaccacctt taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc
     4021 gttgccagaa atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg
     4081 ccaccgatat ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta
     4141 tgttttggca ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc
     4201 aggctggtca aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga
     4261 acgttgccta gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga
     4321 gaaagtagaa gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt
     4381 aacattccgg cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc
     4441 attggcgaga actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga
     4501 tagttaattt tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt
     4561 ggcgaaagca tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg
     4621 ccattttgct aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat
     4681 cagtgcagcg cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt
     4741 accagtgaaa atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa
     4801 aagtgatatt acgcattttt acacactgtg atgaaaaaat ctcccgtcat ttataatgat
     4861 aagtgttttt accacttccc cttttcgtca agatcggcca aaattccacg cttacactat
     4921 ttgcgtactg gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa
     4981 cacttttgtt gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc
     5041 tgaagttagg gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca
     5101 aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa
     5161 acgcgcaacg ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg
     5221 cgtagcctga aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg
     5281 gcctattttg ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc
     5341 ctgattctgg gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg
     5401 gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg
     5461 gcgatgctgg aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa
     5521 gctgacttca ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt
     5581 tatctgattg aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac
     5641 accggcgcag gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg
     5701 ccggaaagct ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag
     5761 caaatcctgt cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca
     5821 atgggcgcac tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg
     5881 atcggttatg ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat
     5941 cagccaaaag attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac
     6001 aaacgtgaag aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg
     6061 gctgacggcc cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc
     6121 tcccgtagcc actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt
     6181 aacgcaggcg acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg
     6241 ttttccagtt caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt
     6301 ggcaccatcc ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt
     6361 acgacaagtc ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag
     6421 gtgtcttcgc gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc
     6481 atcaacgcca gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc
     6541 cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg
     6601 cgcccgtcgg aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttcg
     6661 acatcatcaa aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca
     6721 atattatcca ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc
     6781 tggcgggtga tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca
     6841 gcagcggtca gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct
     6901 tccagctgac gcacggtata gctgaccgca gaaggaacac gatgcagctc ctgtgccgca
     6961 gcgctaaaac taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac
     7021 atagtctgcc tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa
     7081 tttcactccc tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa
     7141 cctgggaaaa gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc
     7201 gatatgtatc tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct
     7261 gtcagtgcca gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg
     7321 ccactctccg accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg
     7381 ttaggtagtc tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt
     7441 gtacaggctg tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat
     7501 tatccttcac agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct
     7561 ccggcactgg ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt
     7621 ttcgccaccc tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc
     7681 acgacgaagg cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct
     7741 aaaacctatc gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg
     7801 ctgaccggtt caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta
     7861 agttatgtcc cgcaaactat cgcgtttctg attggtggtt atggctgtcg cgccgcactg
     7921 cagaaatggc aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc
     7981 attgcgacct gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca
     8041 ttctgtgtga tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg
     8101 cgtcccttcc cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt
     8161 ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc
     8221 accaccacca gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa
     8281 cgttgtgaag aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca
     8341 cactgatcta tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg
     8401 aaattagcgg cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac
     8461 ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc
     8521 cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc
     8581 caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga
     8641 gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg
     8701 aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga
     8761 aaaaccccga tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt
     8821 atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg
     8881 caggtctcga gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc
     8941 gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt
     9001 tgggtaatga cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact
     9061 ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa
     9121 aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactag
     9181 cgcactacat ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac
     9241 agcaaaaaat ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt
     9301 atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg
     9361 gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta
     9421 tattcctgct ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta
     9481 ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg
     9541 aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga
     9601 tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac
     9661 gctttaaacg gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg
     9721 atattcagct ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc
     9781 gctaaaggct attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct
     9841 ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc
     9901 tgagtttgtg gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc
     9961 gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg
    10021 acggtcaggc taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac
    10081 tgactatcct ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc
    10141 acttcagcgg tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat
    10201 ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg
    10261 cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc
    10321 aggcagcaac cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg
    10381 ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg
    10441 gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg
    10501 ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt
    10561 ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt
    10621 ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac
    10681 gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc
    10741 gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt
    10801 gccaccgaca tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt
    10861 ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga
    10921 cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt
    10981 ctcattatgc tggtgctgtg gaatgcaggt tacattatcc gctacatgga aaacatcgat
    11041 ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga
    11101 tatctgttct tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt
    11161 atggtaatgg gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat
    11221 ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg
    11281 tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat
    11341 attcgtaacg aaaaaggcac cgctaagccc gagcctgcgg ttatgaaacg actgattcaa
    11401 ctcggtttgc cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct
    11461 ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt
    11521 agttcactga tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt
    11581 tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg
    11641 gtgggtgtct gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc
    11701 gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg
    11761 gcggcggtat atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt
    11821 tataaagata cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg
    11881 ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc
    11941 ttctggatag gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg
    12001 cggttcctgc aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga
    12061 caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa
    12121 tcccgtcttc gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg
    12181 ttatctcaac gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa
    12241 tttctttcgc actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca
    12301 gttgcacatg cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt
    12361 taccaacaat gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac
    12421 cgccctgctg tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg
    12481 aagcgccgtc cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta
    12541 cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta
    12601 ccgcattttc cagcgtaccg gcatgcactt cagcccgccc ccccacattc accgacaggt
    12661 tttgcgtctt accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc
    12721 cattctcaac aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa
    12781 gcaacgtatc actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat
    12841 tgctggcttc tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg
    12901 aggttgtccc attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat
    12961 gtccttccag tacacgcagg ttaccgccat tttccagcaa caaaccgcag gcataaccct
    13021 gatcaacgct gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac
    13081 taattgcgcc tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata
    13141 gcgtgccatt gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc
    13201 agccagcccc cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc
    13261 cttcagtaag ttgcgtcgcg ccaaggacct cgccggtctt aacaatttgc acattcccgt
    13321 cgctcatcaa gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc
    13381 tttattgcta cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt
    13441 ggcaaacaac tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg
    13501 cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc
    13561 tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc
    13621 tgccgctaat attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta
    13681 gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg
    13741 cgtccgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt
    13801 ccactcggac gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct
    13861 ctgtatccaa tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc
    13921 gctatggcaa ctttgttaca acttcatttt gcttttaatg gcccatttgg cgacgcaatg
    13981 gctgagcagc ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag
    14041 gtatggacag aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa
    14101 aaaagcgccc ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag
    14161 gaagttgtcg ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa
    14221 ctcgcctgac agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt
    14281 ctcaaattct aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt
    14341 acaggtaagt aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca
    14401 ccttattctc gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg
    14461 acgaggccgg tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa
    14521 atattgccag tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc
    14581 aagaagccag ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt
    14641 ttttaccgcg aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc
    14701 aggcacgaca gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc
    14761 aaattacaaa tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg
    14821 atttagcggt gattgccacg ggtcacgtct ggcctgatga agaagaagca accagaacgt
    14881 attttcccag cccgtggtca ggcctgatgg aagcaaaggt cgatgcgtgt aacgtgggta
    14941 ttatgggaac atccttgagc ggactggatg cggcaatggc agtggctatt cagcatggtt
    15001 cgttcattga agatgataaa caacacgtcg tttttcaccg cgataacgca agtgaaaagc
    15061 taaatattac gttgatgtcg cgcacgggta ttttacccga agccgatttc tattgcccta
    15121 ttccctacga gcccttacac atcgtcaccg atcaggcatt aaatgctgag attcaaaaag
    15181 gtgaagaggg ccttttggat cgggtattta gattgatagt agaggaaatc aagtttgctg
    15241 atccagactg gagccaacgc atagccttag agagcctgaa tgtcgattcc tttgctcaag
    15301 cctggtttgc cgagcgcaaa caacgcgacc catttgactg ggcagaaaaa aatctccagg
    15361 aagtcgaacg caataaacga gaaaaacata ctgttccctg gcgttatgtc attctgcgcc
    15421 tgcatgaagc cgtacaggaa attgttcctc atctgaatga acacgaccat aaacggttca
    15481 gtaaaggcct tgccagggtt ttcatcgata attatgcggc aatcccttca gagtctattc
    15541 gtcgactact ggccttacgt gaagcgggga tcattcatat tctcgccctc ggtgaagact
    15601 acgaaatgga aattaatgag tcgcgcaccg tcctgaaaac ggaagacaac agctactcgt
    15661 ttgacgtttt tattgatgcc cgcggacagc gtccgcttaa agtgaaagat attcctttcc
    15721 ctgggctacg cgagcaatta cagaaaacag gggatgaaat ccctgatgtt ggcgaagatt
    15781 atacgttaca gcaacccgaa gatattcgtg gacgcgtagc gttcggcgcg ttgccctggt
    15841 tgatgcacga ccagcctttc gttcagggac ttacggcatg tgcagaaatt ggtgaggcga
    15901 tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg gcgtctttcg tttgattaaa
    15961 ggtaaagcta tcagcactca acaaactgat gctgttcatt aagacgatag cggactccgg
    16021 ttcgaatatt gttttcacct tcaatggcga cggcgaagcg aactctttcg ccgtcatgat
    16081 atgccagtgc ggcgcttccc cctagcccga gaataattga gtcgaccacg cccgtgctca
    16141 ctaccgttga attttcaccg ccggcaatga tgtgtacatt gtcgccgctg ttggcaattc
    16201 tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc attactggcg acatgacacc
    16261 gttcacccaa tgtacaaacg cgtactcgca ttccggtatt cgcaatccga ctactatctc
    16321 cagcgctgct tatccgcgaa gaattaccgg ttacagcaac ccgtgcgcgt tcaccggagc
    16381 tgccaatgtg actgttaaag ccgacacttc caatccgcac gctataaccc atactggcaa
    16441 tttgcgcggc atacccagcg gttgcgattc gggcgttatc ctcacaacag ccgatgcgag
    16501 catgatcgtc gctgcgggca aactgactgc aaaatggtat ctctccgggg tctgtggcca
    16561 gtttttgcat agaggaaact tcctgatgcg cgaagttttc atccgccagc cattttttcc
    16621 acgcatactc aaccagactc tccgcccagt ccgtatatcc ttcctcaatc agtgcatgac
    16681 gaatatcggc ataactaccg ccgcgtggga aatggcgcag gaaccagcga tacatcaccg
    16741 ctccgatacg ccattctcgc aggtctgctc gtgtaatgat cattcgttag tgccggtgat
    16801 agccatcgac catgctttta aaggtttcgt gtggcgtacg ccccgtggtg caaagataaa
    16861 gatgaccgaa gataaaaaag aggcttataa atgccagagc aaaatgcgcc tgcaataacc
    16921 agtatcttac gccaggaaac acatctccca cggcttgcgg atagagacac aacagccccg
    16981 tcaatagtaa caacggcagc aatccataca tgacaccaac ataggcgacc tgctgtaagg
    17041 gattaaattt agactgtgtt gttgccggga aaggatgttc ttccccctgc ataatgccaa
    17101 acaaataaaa tcgcgtttgt tttgccgctc gttccagcca cccctgacga cgaatgcgat
    17161 agtggtgacc attatcccca acggcattga tcagcacaaa gccgagccag catgccagta
    17221 acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt aaccgcagtt gcgcccacca
    17281 tcgcaaagtg atttatcagc ccacttgcca gcaacaatac aaagagtaac gcattcgacc
    17341 agtgccataa tctgaccgct ttgctgtaaa gataaacctt ctcaccatga tcggtggctg
    17401 atttcttcac gccacgagca cgaagcaagg cgtgcagccc cagcaccaac cacatcccaa
    17461 ccagcaatac tccggcaatg atcaaccaca ccggccaaaa ttcgggagtg aactgtggca
    17521 catagttcgc taactggctc tgaaactgtt cagcatgttg cgacgggttc atacattttc
    17581 ctttttaatc aaatgttgac cgaaccggcg atacagatgc ggttttccgg caccaggtag
    17641 ctgatattga tagtatttat tatcctgcaa ccacgcctga atttctgggc tatcttcgcg
    17701 accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa atgggcggaa agccttttgc
    17761 cagtcgggac tcagcgcaga aatcacattt atctgccact ttagtgacag ggttaaggta
    17821 gcgaacctgg tacggacaag cgccaatgca ataactgcag ccgatgcact gcgatttttc
    17881 tacccgcacg atcccctgct catcacgcca cgatgccccc gtcgggcata cgtcgatgca
    17941 cggagcatct tcgcagtgct gacatgactg gcgaaaaaag tgatactgcg tttcgttgtc
    18001 gttatcggtg actggaatgt gtgctatcga taaacggctt ccctgagcgg ggacgtggtt
    18061 agttttacgg catgcgcggg cgcaaatatt acagccattg caccgtgact cgtcatgaat
    18121 catggcatac cgaacttcct tttcttgcct cgtgttcgct aacagagaag acgcagaacc
    18181 agtaaaaaag attactgttc ccatccccag aacaaatttg cgtcgagtaa aggacatctg
    18241 attatcccct aatgcagtgg ctgcaactgt ccaccctatc catgacatct ccctgaaatt
    18301 tcaggggttc tgaaactgct ctacctcgaa ccattcgcat aagccataag ccttgccata
    18361 ggtcataaat cgctcgctaa acaagtacaa caccgccgtc gatgactcca cctgccgtat
    18421 atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg gcttcaatct gcgcttcctg
    18481 gaagtaatat ggcgcttgca tcagcatggc gactttgtaa ggacgaggat acgtctggca
    18541 ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc tgctccacaa cttgcaaaga
    18601 catcgccgcg tagttttcac tcatggcctg cgttgagtaa tagtaatcat cctgacttcc
    18661 cgtcatacaa gcgatatccg cgtagtctcc gttctgctta atgctttcca gcaaaactga
    18721 cagttcctct tctgcaaccg aatagggtgg cgagagaaac acggtgcgcg ccaccagctg
    18781 cccagccgca gaatgctggc gaatgaactc agccaggatt tcagcaggtt ctggttcggt
    18841 taccgtatca acggggtgac gcatcccctg caaccattgc cgcttcgcct cgtcaacttc
    18901 gctgactttc gctaacggca attcatcctg atggttcatc ttacccattc cttacgcagg
    18961 cagtagattg tgtgctgcca gatcggcggc aatatcttcc agccccagac gctgcaatgt
    19021 ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt tctttgtaga acatcgtgag
    19081 cgaagcatgc atatcgtcac gatccatttt gtcagtacct tcagtaaaga ccgggatctg
    19141 cggatccttg tcgaaaaccc aggaacagat aagatcgtgt tcattacgca tatctttggt
    19201 ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag atacgctctg cagctaaatc
    19261 taatttttcc tgagtcatct cttcgccggt gatcgctttg aagaatttgg cttaaagcgc
    19321 cagatcgccc cggtaattac ggcttttcag cggcgaaacg gtcattggcc agacccagtt
    19381 gcacagagtg acggcgttat gcaaacagac ccgcaacagc gaccactttg catatttaat
    19441 ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg taagcatctt cagaaccaaa
    19501 aagttctttc gccacttcac gttgcagttt caatggcaag ccggaaccaa taaagttgat
    19561 atgggtatgc gtcatgcagt cacggttgaa catacagtta acaatggaac cgacctgcgc
    19621 tgacgcttca ttggcatggt gaaccggata gccaaacggc gaccagagtt tatttttcgc
    19681 gtagccccag tactctttac ccaaattcca gcgttctgcg atggcatatg aaccatcagc
    19741 caggtgactc agctcaccca cacgatgcgc cagacggtag taaaaatctt taatgaagtt
    19801 aacgtcaccc gcttccagtt gatcccagcg aatttctgca tactcttcag ctggcagaac
    19861 acgcttgaac acacctttgc tgtaacaata agtaaagtcg cgatgcaact gcccgtagtt
    19921 acaccatagg ccgtagtcat cgaacaaatt cagaccaacc aggttaccaa tcacacgacc
    19981 atcgtcttta tcttcaaaat ctttcggccc gttcgggaag atggtggtat ggacaaagtt
    20041 tgctacacaa gtgttaccac ctgtgctggg cacgccaaac tctttcaccc gaggaatatt
    20101 catttgggtc atacaacgga tcgggcaaga gtggcagccg ctcattttta ctgtgtattt
    20161 ctctgccgcc ggtcctaagt caaaaacgga tttataggta cgaaagccga ccgtattctg
    20221 attgcctggc ggaatttcac ccgtttcaat cggaccacct tcagccgcgc cccaaaacag
    20281 ccctttacgt gctgtccagc gtgacttggg atctgaatac tctgcccacg attgtggcgt
    20341 acttggcacg acatggttgt tattcgcacc aataagttca gtcatcatgt aatcattgag
    20401 acgcttcatc tcctgacgat cggcaatgtt gacccctttc gtcccttcaa ccgcaatcgc
    20461 tttcaggttt ttcgaaccca ttattgcgcc agttcccgca ccgccgctgt ggttacggct
    20521 attcaacatg ccagagagag gaataaggtt ttccccagcc tgaccaatag ccgccacaca
    20581 ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt gcgcgcgtcc cttttcccca
    20641 taagaaatcg gctttttcca ggctaacttt gtcatcttta atcttcagcc ataccggtga
    20701 tttcgccttc ccttcgataa taatgacgtc gtatccagcg aatttcattt gcgctgcaaa
    20761 aaagccaccc atatgggcat cgcgactaaa tttcctttgg taaaagtaga aagtgaggtg
    20821 atatttacgc gagaactaca gggggcacca gatccagtta atgggccggt agcaaagact
    20881 aatttattcg cttcatcgaa aggtttcgtg cctagcggta cttcgtcata cataattttg
    20941 tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc gagggtaata
    21001 tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc catgattttt
    21061 tcctttgcaa gattcacaca gtaatatctt tccactcgat aatttttaac gcccctgttg
    21121 ggcaggcgtt tgcgcattca ccgcataaca cacatttcga ggatttttta ctttcggtat
    21181 ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca atacaacgtt
    21241 tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca atcgggcaga
    21301 cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag ttgagatcgc
    21361 catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg cgatggattt
    21421 tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa gagatttcac
    21481 agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg ggtgtattta
    21541 tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt gacagtaacg
    21601 caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat tctaatcgcg
    21661 ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca tcctcttcgc
    21721 aaacacagtg ctgacaattc agcagcctga tattaagcat tcaactaata cgctgctgac
    21781 tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa gatttaacgc
    21841 cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg agcccttaac
    21901 attgatcgtt atcaattaaa attacaaagc aagaaattat aagcgaactg atatttatta
    21961 tcatttgaaa taaatttaac ttaagcgaca atcatttgtg acgttcattg cagaaaataa
    22021 aaataacatt atcgctataa attaaaatac ataccgaaat tcgtataatt tcagcagttg
    22081 ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg
    22141 cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc
    22201 cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac
    22261 aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct
    22321 gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt
    22381 atcatgccaa ct
//
LOCUS       DADLDT020000064        22106 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000064 DADLDT020000000
VERSION     DADLDT020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.064"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="JQH22_002390"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="JQH22_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFB2206679.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="JQH22_002391"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="JQH22_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HFB2206680.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="JQH22_002392"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="JQH22_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFB2206681.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="JQH22_002393"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="JQH22_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFB2206682.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="JQH22_002394"
     CDS             3251..5011
                     /locus_tag="JQH22_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HFB2206683.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="JQH22_002395"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="JQH22_002395"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFB2206684.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="JQH22_002396"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="JQH22_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFB2206685.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="JQH22_002397"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="JQH22_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFB2206686.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="JQH22_002398"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="JQH22_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFB2206687.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="JQH22_002399"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="JQH22_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFB2206688.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="JQH22_002400"
     CDS             complement(9679..11586)
                     /locus_tag="JQH22_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2206689.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="JQH22_002401"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="JQH22_002401"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFB2206690.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="JQH22_002402"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="JQH22_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFB2206691.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="JQH22_002403"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="JQH22_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFB2206692.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="JQH22_002404"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="JQH22_002404"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFB2206693.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="JQH22_002405"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="JQH22_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="JQH22_002406"
     CDS             complement(17598..18113)
                     /locus_tag="JQH22_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFB2206694.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="JQH22_002407"
     CDS             complement(18121..18663)
                     /locus_tag="JQH22_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2206695.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="JQH22_002408"
     CDS             complement(18761..19744)
                     /locus_tag="JQH22_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2206696.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..>22106)
                     /locus_tag="JQH22_002409"
     CDS             complement(19735..>22106)
                     /locus_tag="JQH22_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFB2206697.1"
                     /translation="VLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPA
                     FKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINA
                     LFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYL
                     QRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSN
                     AQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVP
                     LLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAM
                     FGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYS
                     TSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGL
                     FYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSL
                     PLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGY
                     NSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSL
                     GETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLE
                     NAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDD
                     GSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5479 a         5794 c         5194 g         5639 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaa
//
LOCUS       DADLDT020000065        21387 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000065 DADLDT020000000
VERSION     DADLDT020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.065"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..134)
                     /locus_tag="JQH22_002410"
     CDS             complement(<1..134)
                     /locus_tag="JQH22_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206698.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="JQH22_002411"
     CDS             complement(236..1216)
                     /locus_tag="JQH22_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFB2206699.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="JQH22_002412"
     CDS             complement(1224..1934)
                     /locus_tag="JQH22_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFB2206700.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="JQH22_002413"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="JQH22_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFB2206701.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="JQH22_002414"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="JQH22_002414"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFB2206702.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="JQH22_002415"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="JQH22_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFB2206703.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="JQH22_002416"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="JQH22_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFB2206704.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="JQH22_002417"
     CDS             8511..8621
                     /locus_tag="JQH22_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFB2206705.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="JQH22_002418"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="JQH22_002418"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFB2206706.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="JQH22_002419"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="JQH22_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFB2206707.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="JQH22_002420"
     CDS             11503..12135
                     /locus_tag="JQH22_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HFB2206708.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="JQH22_002421"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="JQH22_002421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFB2206709.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="JQH22_002422"
     CDS             15667..16599
                     /locus_tag="JQH22_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFB2206710.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="JQH22_002423"
     CDS             complement(16640..18070)
                     /locus_tag="JQH22_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFB2206711.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="JQH22_002424"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="JQH22_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFB2206712.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="JQH22_002425"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="JQH22_002425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFB2206713.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="JQH22_002426"
     CDS             complement(19931..20200)
                     /locus_tag="JQH22_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFB2206714.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="JQH22_002427"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="JQH22_002427"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFB2206715.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="JQH22_002428"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="JQH22_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFB2206716.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DADLDT020000066        21274 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000066 DADLDT020000000
VERSION     DADLDT020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.066"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..444)
                     /locus_tag="JQH22_002429"
     CDS             complement(<1..444)
                     /locus_tag="JQH22_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HFB2206717.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="JQH22_002430"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="JQH22_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFB2206718.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="JQH22_002431"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="JQH22_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFB2206719.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQH22_002432"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQH22_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFB2206720.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="JQH22_002433"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="JQH22_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFB2206721.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="JQH22_002434"
     CDS             complement(5214..6812)
                     /locus_tag="JQH22_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFB2206722.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQH22_002435"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQH22_002435"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFB2206723.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="JQH22_002436"
     CDS             complement(8491..9069)
                     /locus_tag="JQH22_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFB2206724.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQH22_002437"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQH22_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFB2206725.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="JQH22_002438"
     CDS             10508..10687
                     /locus_tag="JQH22_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFB2206726.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="JQH22_002439"
     CDS             complement(10807..11166)
                     /locus_tag="JQH22_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFB2206727.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="JQH22_002440"
     CDS             complement(11528..11872)
                     /locus_tag="JQH22_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFB2206728.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="JQH22_002441"
     CDS             12004..13914
                     /locus_tag="JQH22_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFB2206729.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="JQH22_002442"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="JQH22_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFB2206730.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="JQH22_002443"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="JQH22_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFB2206731.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="JQH22_002444"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="JQH22_002444"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFB2206732.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="JQH22_002445"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="JQH22_002445"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFB2206733.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLDVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="JQH22_002446"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="JQH22_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HFB2206734.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="JQH22_002447"
                     /pseudo
     CDS             complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="JQH22_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20409..20975)
                     /locus_tag="JQH22_002448"
     CDS             complement(20409..20975)
                     /locus_tag="JQH22_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2206735.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21274
                     /locus_tag="JQH22_002449"
     CDS             21187..>21274
                     /locus_tag="JQH22_002449"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2206736.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5274 c         5466 g         5268 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcacc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat tgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cgctctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggacgt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gggataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgagcat agctcttgaa ttgagcattt ttctgcggta
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agag
//
LOCUS       DADLDT020000067        21254 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000067 DADLDT020000000
VERSION     DADLDT020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.067"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..792
                     /locus_tag="JQH22_002450"
     CDS             <1..792
                     /locus_tag="JQH22_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFB2206737.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(852..2150)
                     /gene="eno"
                     /locus_tag="JQH22_002451"
     CDS             complement(852..2150)
                     /gene="eno"
                     /locus_tag="JQH22_002451"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFB2206738.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="JQH22_002452"
     CDS             complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="JQH22_002452"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HFB2206739.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="JQH22_002453"
     CDS             complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="JQH22_002453"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFB2206740.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4990..7224)
                     /locus_tag="JQH22_002454"
     CDS             complement(4990..7224)
                     /locus_tag="JQH22_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFB2206741.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="JQH22_002455"
     CDS             complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="JQH22_002455"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFB2206742.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8630..11386
                     /gene="barA"
                     /locus_tag="JQH22_002456"
     CDS             8630..11386
                     /gene="barA"
                     /locus_tag="JQH22_002456"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFB2206743.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLVLESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="JQH22_002457"
     CDS             complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="JQH22_002457"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFB2206744.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12979..14319)
                     /locus_tag="JQH22_002458"
     CDS             complement(12979..14319)
                     /locus_tag="JQH22_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFB2206745.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="JQH22_002459"
     CDS             complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="JQH22_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFB2206746.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16107..16556)
                     /locus_tag="JQH22_002460"
     CDS             complement(16107..16556)
                     /locus_tag="JQH22_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFB2206747.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16574..17356)
                     /gene="truC"
                     /locus_tag="JQH22_002461"
     CDS             complement(16574..17356)
                     /gene="truC"
                     /locus_tag="JQH22_002461"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFB2206748.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17356..17685)
                     /locus_tag="JQH22_002462"
     CDS             complement(17356..17685)
                     /locus_tag="JQH22_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFB2206749.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18307..18852)
                     /gene="syd"
                     /locus_tag="JQH22_002463"
     CDS             complement(18307..18852)
                     /gene="syd"
                     /locus_tag="JQH22_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFB2206750.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18920..19768
                     /gene="queF"
                     /locus_tag="JQH22_002464"
     CDS             18920..19768
                     /gene="queF"
                     /locus_tag="JQH22_002464"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFB2206751.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19880..21244
                     /gene="ppnN"
                     /locus_tag="JQH22_002465"
                     /gene_synonym="ygdH"
     CDS             19880..21244
                     /gene="ppnN"
                     /locus_tag="JQH22_002465"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HFB2206752.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5225 a         5670 c         5074 g         5285 t
ORIGIN      
        1 gttgcagccc aaccaactat tgtcccacaa ttacagcaac aagttacggg tcttacgagt
       61 agcttaaact cacaagaaaa gaaagaactg acgcacaagt tagaatctat tttcaataat
      121 acccaagtgc aaattgctgt attaattgtt tccacaacca aagatgagac aattgaacaa
      181 tatgctacaa gagtttttga caattggcgt ttaggagatg ccaaacgtaa tgatgggata
      241 ctgatcgttg ttgcctggtc ggatcgcact gtccgcatcc aggtaggtta tgggctggaa
      301 gaaaaggtaa ccgatgctct ggctggagat atcatccgta gcaacatgat acccgcattt
      361 aaacaacaaa aattagctaa gggattagag ttagctataa tcgctttgaa taaccaactc
      421 acttcacaac accaatatcc ggctaatcct tcagaaagtg aatcaacgtc ttccactgat
      481 cattattact ttgctatttt ttgggtattt gcagtgatgt tcttcccttt ctggtttttt
      541 catcaaggca gtaatttttg tcgcgcatgt aaaagtggag tctgtatttc agcaatccat
      601 cttttagatt tattcctgtt ctccgataaa actttttcca ttgctgtatt ttccttcttt
      661 tttactttca ccatatttat ggtctttacc tgtttatgtg tacttcagaa aagagcatct
      721 ggtagaagtt atcattcaga caacagcggt tccgctggag gctcaagtgg cggcgcatcc
      781 ggccgctggt aactccacca taaaaaatgc cagcccgaag gctggcatta tcaaatcaga
      841 taaagtcaga cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg
      901 cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc
      961 atagaaccag ttttgatctg gcctgcagca gtaccaacag ccagatcagc gatggtagca
     1021 tcttcagttt caccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc
     1081 ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag
     1141 ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg
     1201 tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag
     1261 tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc
     1321 aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat
     1381 ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc
     1441 agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa
     1501 cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc
     1561 agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt
     1621 ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca
     1681 ccaccgttga tgatgttcat catcggaacc ggcatggagt atttgcccgg agtgccgttc
     1741 agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta
     1801 gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc
     1861 aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc
     1921 tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg
     1981 gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga
     2041 gcagctgcca tgccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta
     2101 ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc
     2161 tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaagg tacgcgttgc
     2221 cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca
     2281 aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg
     2341 caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg
     2401 gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact
     2461 tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtatagctg gcgaaccagg
     2521 ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc
     2581 ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca
     2641 accgggtact tacagtctgg cacaaattct gtagagttgg cgttctccat gttggcaaca
     2701 tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata
     2761 ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa
     2821 ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca
     2881 tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc
     2941 agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg
     3001 atggtgactt cacttaccgg attcgcttct tcgaagataa cctgttccca ttcggacaga
     3061 tttgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat
     3121 ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt
     3181 tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggtac agcgcgatct
     3241 gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc
     3301 tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc
     3361 ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatagc ttcgaggaac
     3421 ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct
     3481 tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg
     3541 gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc
     3601 gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg
     3661 tccaagtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc
     3721 atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac attgaggcca
     3781 cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg
     3841 accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa
     3901 aagacgatgg aataaccaag acgggaaagc agtatacccg aacatgacct gtgccacaaa
     3961 ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc
     4021 ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat
     4081 ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga
     4141 cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac
     4201 gctccacttc gcgaaaacga cgctcgaatt tttcgttcac tttttgcaat gcgatttctg
     4261 ctttcgtccc taaatggcga gccagattaa ccgtggcaaa cagcaggtcc cccatttcct
     4321 cctccagttt agcctggtcg acaacagcct gccgcgcctc gtacatcacc tcgtcgatct
     4381 cttcgtagac tttatcaacc accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc
     4441 aacgtttctg gattttttgc gcccgcatta aagccggtaa actacgagga atatcgtcca
     4501 gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa
     4561 gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta
     4621 atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca
     4681 tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa
     4741 aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa
     4801 tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct
     4861 gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct
     4921 gtcaggtgta ataccttcga gtaaaagata tcgctggata cggctgacgc catatccagc
     4981 tatgtaagac taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     5041 tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     5101 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     5161 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5221 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5281 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5341 tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5401 gtggtgcatc aggttaccaa caccttcaac caccacacga ccgttatctt tactgcggtt
     5461 ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc
     5521 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5581 gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg gcagcagatg
     5641 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5701 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     5761 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5821 ctgtttctgg gtgataattt caatctggtc gcccatttgc agctggtagg tgaacggcac
     5881 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5941 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     6001 gacatacacc cggtcgtcaa acacctgact acgtacttcg tcgagcattt cgccggaatc
     6061 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     6121 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6181 ctccgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt ttccacccgg
     6241 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacgtaatc
     6301 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     6361 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     6421 gaggttcttt ttctgcattt tgcgccagat gctgtagatg tgtttcggac gaccatacac
     6481 ttcagcttta acgccttcag ctttcatctc agcgcgcaga tggccgacaa actcttcgat
     6541 atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt
     6601 cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     6661 acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta cacgttcatc
     6721 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6781 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6841 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6901 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6961 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     7021 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     7081 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     7141 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7201 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7261 gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7321 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7381 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7441 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7501 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7561 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgca
     7621 ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7681 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7741 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7801 aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7861 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7921 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7981 gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct gcccagacat
     8041 gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca ttgtttgacg
     8101 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     8161 aagtagttca gacttaaacg cgcgcggcgg cgatagcccc agggaacatc ggcgatcact
     8221 tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8281 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8341 gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8401 ttatcttcag taacagcaac ttccgcgttt tcctgcggca ataatccggg gataaataac
     8461 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8521 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8581 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8641 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8701 gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8761 cggtgctagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8821 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8881 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8941 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     9001 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     9061 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgcttgagct
     9121 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcct gcgtgatgat
     9181 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9241 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9301 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9361 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9421 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc tggcgaaaaa
     9481 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9541 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9601 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9661 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctggttc tggaaagtat
     9721 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9781 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9841 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9901 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9961 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
    10021 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccgggct
    10081 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
    10141 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10201 tattatcgaa gggccatcca tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc
    10261 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10321 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10381 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10441 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagttaatg ctgaaaaact
    10501 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10561 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10621 taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga aacttatcgg
    10681 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcagt
    10741 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgccggacat
    10801 ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcgac aaacgccggt
    10861 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10921 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10981 caagcctggc agcggtattt cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    11041 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    11101 tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    11161 tgaggaacag ctggctggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11221 tggcagttgc ggctatagcg gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca
    11281 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11341 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11401 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11461 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11521 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11581 aaccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11641 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11701 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11761 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    11821 tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca gtaattttac
    11881 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11941 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    12001 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    12061 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    12121 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    12181 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12241 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12301 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12361 catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    12421 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12481 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12541 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12601 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12661 cgcgcccact agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12721 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12781 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12841 tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatgac
    12901 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    12961 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    13021 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt
    13081 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    13141 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13201 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13261 cacatgggta aatatcgcca gagagatatc gaaatggttg ttggaatggc agccccaggt
    13321 cagcccccag tcgtcgcaaa gctgcgccac gcggactgca ccggaaagcg tccagaagtg
    13381 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13441 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgtcgaa actccgccat
    13501 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13561 atcattcagc cctttgcata aagaaatggc ttcatcaagc agccatgcac cgttgggatc
    13621 aacggtaatc cgcgcatccg ggaagctttt cttcaatgca cgaacagtgt cgatttcttg
    13681 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13741 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13801 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13861 tatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13921 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13981 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    14041 ggctgcctga ttacctttat gtacctgctg aaccacttta ttcaggcgcg caacttcctg
    14101 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    14161 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acgatattgc gggtgaagta
    14221 tgcgttatgc gcaccaccaa tattgagcaa catgctgtca tgtccggcga ccggaatgac
    14281 tttcatatca gtaataacag gactggattg tgtcgccatc ttattgcccc gcgacaggtt
    14341 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaggg
    14401 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    14461 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14521 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14581 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14641 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14701 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataattcca cccagcacac
    14761 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14821 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14881 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga
    14941 tctgccccca cttcacgctg aacggcactt taactttggt gctttgctga tccatattga
    15001 tcagcgcgcc acccgcggcg atatactcca gctctttctg atttaccccc ggatgttgat
    15061 ttggctcgtg gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa
    15121 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15181 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgca
    15241 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15301 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15361 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15421 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15481 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15541 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15601 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15661 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15721 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15781 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15841 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15901 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg
    15961 aagaaataaa gaagagtatg aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc
    16021 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    16081 aacgacattt accgctcgct gacatatcag gacaacagcg tgccccactg ttcgacccac
    16141 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16201 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa
    16261 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16321 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16381 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    16441 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16501 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16561 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16621 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16681 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16741 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16801 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatatc
    16861 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16921 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16981 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    17041 ccaccagtgg ataatccaac accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    17101 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    17161 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17221 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17281 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17341 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17401 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17461 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17521 cagcccccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17581 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17641 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17701 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17761 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17821 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17881 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17941 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    18001 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    18061 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    18121 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18181 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18241 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    18301 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18361 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18421 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18481 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18541 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18601 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18661 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18721 agttgaaata atgcacggtg aaggaacacc gtacaattcc tcacttaacg gccagctttt
    18781 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18841 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18901 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18961 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    19021 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    19081 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    19141 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19201 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19261 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19321 tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg atgaccagga
    19381 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    19441 aaaagtagtg gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19501 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19561 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19621 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
    19681 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19741 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19801 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19861 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19921 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19981 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    20041 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    20101 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    20161 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccataatac
    20221 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20281 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    20341 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct
    20401 gggcctgcgt gagctgaata tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat
    20461 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    20521 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20581 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20641 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20701 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20761 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
    20821 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20881 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
    20941 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    21001 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    21061 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    21121 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21181 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21241 gtaatctccc ctcc
//
LOCUS       DADLDT020000068        21234 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000068 DADLDT020000000
VERSION     DADLDT020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21234
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.068"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="JQH22_002466"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="JQH22_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFB2206753.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="JQH22_002467"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="JQH22_002467"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFB2206754.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JQH22_002468"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JQH22_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFB2206755.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JQH22_002469"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JQH22_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFB2206756.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JQH22_002470"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JQH22_002470"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFB2206757.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="JQH22_002471"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="JQH22_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFB2206758.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11272
                     /gene="ftsK"
                     /locus_tag="JQH22_002472"
     CDS             7244..11272
                     /gene="ftsK"
                     /locus_tag="JQH22_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFB2206759.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            11427..12038
                     /gene="lolA"
                     /locus_tag="JQH22_002473"
     CDS             11427..12038
                     /gene="lolA"
                     /locus_tag="JQH22_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFB2206760.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12049..13392
                     /gene="rarA"
                     /locus_tag="JQH22_002474"
                     /gene_synonym="mgsA"
     CDS             12049..13392
                     /gene="rarA"
                     /locus_tag="JQH22_002474"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFB2206761.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13483..14775
                     /gene="serS"
                     /locus_tag="JQH22_002475"
     CDS             13483..14775
                     /gene="serS"
                     /locus_tag="JQH22_002475"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFB2206762.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15015..17459
                     /gene="dmsA"
                     /locus_tag="JQH22_002476"
                     /pseudo
     CDS             15015..17459
                     /gene="dmsA"
                     /locus_tag="JQH22_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            17470..18087
                     /gene="dmsB"
                     /locus_tag="JQH22_002477"
     CDS             17470..18087
                     /gene="dmsB"
                     /locus_tag="JQH22_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HFB2206763.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18089..18880
                     /locus_tag="JQH22_002478"
     CDS             18089..18880
                     /locus_tag="JQH22_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HFB2206764.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(18916..19542)
                     /locus_tag="JQH22_002479"
     CDS             complement(18916..19542)
                     /locus_tag="JQH22_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFB2206765.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19857..21005
                     /locus_tag="JQH22_002480"
     CDS             19857..21005
                     /locus_tag="JQH22_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206766.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         5249 a         5319 c         5614 g         5052 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaagcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tttgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgtacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcggcgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg cgccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccttgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctagtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgtat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctta acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atctggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttgctgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgccttc tggaggcatt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggcag cgggcctgac gttgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa cattctcacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga gtatgaagac gacgaagagt atgaagatga
     7921 aaatcacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag acgctgcgtt
     8041 gttctccggt aagcggatgg atgatgacga agagattacc tacactgcac gcggtgtggc
     8101 tgccgacccg gacgacgtcc tattttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gtcgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcgcc ggttgaacct gtgactcaga cgccgcctgt
     8281 tgcctctgtt gatgttccac cttcgcaacc tacagtagcc tggcagcctg taccgggtcc
     8341 acaaacggga gagccggtta ttgctcctgc accggaaggt tacccacagc agtcacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa ccagtacagc cgcagcagcc
     8461 gtattatgca cctgcagctg aacaacctgc gcaacagccg tattatgccc ctgcgccaga
     8521 acaaccggtg gcaggtaacg cctggcaagc cgaagagcag caatccactt ttgctccaca
     8581 gtctacatac cagactgagc aaacttatca gcagccagcc gctcaggagc cgttgtacca
     8641 acagccgcaa cccgttgaac agcagcctgt tgtggagcct gaacccgttg tagaagagac
     8701 aaaacccgcg cgtccgccgc tttactattt tgaagaagtg gaagagaagc gagcccgtga
     8761 acgtgaacaa cttgcggcct ggtatcaacc gattccagaa ccggttaaag aaccagaacc
     8821 gatcaaatct tcgctgaaag caccttctgt tgcagcagta cctccagtag aagccgctgc
     8881 cgctgtttct ccgctggcat ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc
     8941 aaccgttgcc gcgccagtct tcagtctggc aaatggcggt ggaccgcgtc cgcaggtcaa
     9001 agaggggatt ggcccgcagt tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga
     9061 attggcgtct tacggtatta agctgccctc acagcgtgcg gcggaagaaa aagcccgtga
     9121 agcccagcgc aatcagtatg attctggcga tcagtataac gatgatgaaa tcgatgcgat
     9181 gcagcaggat gaactggcac gtcagttcgc ccagacacag cagcaacgct atggcgaaca
     9241 gtatcaacat gatgtgcccg taaacgcaga agatgcagat gctgcggcag aggctgaact
     9301 ggctcgtcag tttgtgcaaa ctcaacaaca acgttattcc ggcgaacaac cggctggggc
     9361 gaatccgttc tcgctggatg attttgaatt ttcgccaatg aaagcgttgc tggatgatgg
     9421 tccacacgaa ccgctgttta cgccaattgt tgaacctgta cagcagccgc aacaaccggt
     9481 tgcaccacag cagcagtatc agcagccaca acagccggtt gcgccacagc cgcagtatca
     9541 gcagccacaa cagcaggttg cgccgcagcc acaatatcag cagccgcaac aaccggttgc
     9601 gccacagcag caatatcagc agccgcaaca accagttgcg ccgcagccgc agtatcaaca
     9661 gccgcaacaa ccggttgcac cgcagccgca gtatcagcag ccacaacagc cagttgcgcc
     9721 acagccgcag gataccctgc ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt
     9781 gcacaaaccg acgacgccgc tgccttctct ggatttgctg acaccgccgc cgagcgaagt
     9841 ggagccggta gatacctttg cgcttgaaca aatggcgcgt ctggtggaag cgcgtctggc
     9901 tgatttccgt attaaagccg atgtcgttaa ttactctccg gggccagtta tcactcgctt
     9961 tgaacttaac ctggctcctg gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct
    10021 tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta
    10081 tgtaggtctg gagttaccaa ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga
    10141 taacgccaaa ttccgcgata atccgtcgcc attaaccgtg gtgctgggta aagatatcgc
    10201 cggtgagccg gtggttgccg atctggcgaa aatgccgcac ttgttggttg cggggactac
    10261 cggttccggt aaatctgtcg gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca
    10321 gccagaagat gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt cggtttatga
    10381 aggcattccg cacctgttaa cggaagtcgt tactgatatg aaagatgccg ccaacgcgct
    10441 gcgctggtgt gttaacgaga tggagcgtcg gtataaactg atgtctgcgc tgggtgtgcg
    10501 taatctggcg ggttataacg aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc
    10561 agacccgtac tggaagccgg gtgacagtat ggatgcccag catccggtgc tgaaaaaaga
    10621 accatacatt gtcgtgttgg ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa
    10681 agtggaagag ctgatagctc gtctggcgca aaaagcccgt gccgcgggta tccacctcgt
    10741 actggcaact cagcgtccat cggttgatgt tattactggt ctgattaaag cgaatattcc
    10801 gacccgtatc gcctttaccg tatccagtaa gattgactca cgtaccattc ttgatcaggc
    10861 tggcgcggaa tcactgctgg gtatggggga tatgctctac tctgggccga actccacgtt
    10921 gccggtacgt gtccatggtg cttttgttcg cgatcaggaa gttcatgccg tggtgcagga
    10981 ctggaaagcg cgtggtcgcc cacagtatgt tgatggcatc acctccgaca gcgaaagcga
    11041 aggtggtgcg ggtggtttcg atggcgctga agaactggat ccgttgttcg atcaggcggt
    11101 gcagtttgtc actgaaaaac gcaaagcgtc aatttctggc gtacagcgtc agttccgcat
    11161 tggttataac cgtgcagcgc gtattatcga acagatggaa gcgcagggga ttgtcagcga
    11221 acaggggcac aacggtaatc gtgaagtgct ggccccaccg ccgtttgact aattaatgca
    11281 ttgccggata aggcgcggta gcgtcgcatc cggcactcta tcaactgaaa attcagtatt
    11341 ttcttctttc ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccgatcg
    11401 ggagtgacgt aatttgagga ataatgatga aaaaaattgc tatcacctgt gcattactct
    11461 caagcttagt agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agccgcctgg
    11521 ataaagtcag cagcttccac gccagcttca cacaaaaagt gactgacggt agcggcgcgg
    11581 cggtgcagga aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata
    11641 tgacacaacc tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc
    11701 cgttcgttga gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta
    11761 tgctgattgc ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg
    11821 atgactttgt cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg
    11881 tgggacgtga tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca
    11941 gttatcagct gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc
    12001 cgccgcaagg cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg
    12061 tcgctcgatt tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat
    12121 ttagcacagt atatcggtca gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct
    12181 atcgaagccg ggcatttgca ttctatgatc ctctgggggc caccgggtac cggcaaaaca
    12241 accctcgctg aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc
    12301 acctctggcg tgaaagagat tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca
    12361 ggtcgccgca ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat
    12421 gcatttctgc cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac
    12481 ccgtcgtttg agcttaattc ggcactgctt tcccgtgccc gtgtttatct gttgaaatcc
    12541 ctgagtacag aggatattga gcaagtacta actcaggcga tggaagacaa aacccgtggc
    12601 tatggtggtc aggatattgt tctgccagat gatacacgac gtgccattgc agaactggtg
    12661 aatggcgacg cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc
    12721 tatgatagcg gtaagcgggt cctgaagcct gaattactga tcgaaatcgc cggtgaacgt
    12781 agcgcccgct ttgataacaa aggcgatcgc ttttacgatc taatttccgc actgcataag
    12841 tcggtacgtg gtagcgcacc cgatgcggcg ctgtactggt atgcgcgaat tattaccgct
    12901 ggtggcgatc cgttatatgt cgcgcgtcgc tgtctggcga ttgcgtctga agacgtcggt
    12961 aatgccgatc cacgggcgat gcaggtggca attgcggcct gggattgctt tactcgcgtt
    13021 ggcccggcgg aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca
    13081 aaaagcaacg ctgtctacac tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgcccg
    13141 gattatgacg tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc
    13201 tacgggcagg aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt
    13261 tacttcccgc cggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa
    13321 ggcaagattg gtgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa
    13381 cgctaccgtt aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct
    13441 gtggccacat ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg
    13501 ctgcgtaatg agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat
    13561 gtagataagc tgggcgctct tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac
    13621 ctgcaagcgg agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa
    13681 gatatcgagc ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa
    13741 gccgagctgg atgctttaca ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg
    13801 cctgcagatg aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagccgctgg
    13861 ggtaccccgc gtgagtttga ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac
    13921 tctggcctcg actttgcagc tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa
    13981 gggcagattg ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa
    14041 cagcatggct acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt
    14101 acgggtcagc tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa
    14161 gcagacacca gtaactatgc gctgatccca acggcagaag ttccgctgac taacctggtg
    14221 cgcggtgaaa tcatcgatga agatgatctg ccaattaaga tgatcgccca caccccatgc
    14281 ttccgttctg aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag
    14341 ttcgacaaag ttgaaatggt gcagatcgtg cgcccagaag actcaatggc ggcgctggaa
    14401 gagatgactg gtcatgcaga aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc
    14461 atcctttgca ctggcgacat gggctttggc gcttgcaaaa cttacgacct ggaagtatgg
    14521 atcccggcac agaacaccta ccgtgagatc tcttcctgct ccaacgtttg ggatttccag
    14581 gcacgtcgta tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat
    14641 accctgaacg gttctggtct ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat
    14701 cagcaggctg atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg
    14761 gaatatattg gctaataccc aatttttctg aatctaaaaa gcgcctttag ggcgcttttt
    14821 ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga
    14881 gtgaaaatct accgatctct ttgattttca aattattcta tgtatacaag cctatatagc
    14941 gagctgctat agaaataatt acacaatacg gtttgttact ggaatcaatc gtgatcaagc
    15001 ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggagagtc
    15061 gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa
    15121 cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcagacg
    15181 aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc
    15241 acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg
    15301 acggcctaca ctaggttcgc gcctgcctgc gtgggcgttc catgcgtcac cgtgtctaca
    15361 atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg
    15421 agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca
    15481 aagagtacgg caacgagtct atctatctga actatggcac cggtacgctg ggcggcacca
    15541 tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg
    15601 gttatcttaa ccattacggt gactactctt ccgcgcaaat tgcggaaggt ttgaaccata
    15661 cctacggcgg ctgggcagat ggtaacagcc cgtcggatat cgaaaacagt aaattggtag
    15721 tgctgtttgg taataaccca ggcgaaacgc gaatgagtgg cggtggggtg acttactatc
    15781 ttgaacaggc acgccagaaa tctaatgctc gcatgatcat catcgatccg cgctataccg
    15841 acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccaggaaca gatgccgcac
    15901 tggttaacgg tctggcgtac gtcatgatca ctaaaaacct ggtggatcag gcattcctcg
    15961 ataaatattg cgttggctat gacgagaaaa ccctgcccgc cagtgcgccg aaaaatggtc
    16021 attataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaaca ccggaatggg
    16081 cctcgcaaat caccggtgtt ccggcagaca aaatcatcaa actggctcgt gaaatcggca
    16141 gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct aacggtgaaa
    16201 tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt attaatggag
    16261 ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg ccgaccttgg
    16321 aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc
    16381 cggaaatgac ggcgctgcgt gatggtgtgc gcgggaaaga taagctggat gtgccgatca
    16441 aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta
    16501 cccatgaaat cctccaggat gataagaagt gcgagctgat tgtggttatc gactgccaca
    16561 tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga
    16621 tggactttgc actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg
    16681 tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac
    16741 gtcttggcgt tgagcaacag tttactgaag gccgtaccta ggaagagtgg atgcggcatc
    16801 tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc
    16861 aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa gccttccgtg
    16921 aagatccgca ggcaaaccca ctgactacgc catcgggcaa aattgagatt tattcgcagg
    16981 cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc
    17041 cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc
    17101 agcttacagg tttccactat aaatctcgcg ttcactcaac ttacagcaac gttgatgtgc
    17161 tgaaagcggc ttgccgtcag gaaatgtgga tcaacccgct tgatgcccaa aaacgcggta
    17221 tccacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat attgaggcga
    17281 aagtgacgcc acgaatgatg ccgggcgtgg tcgcactggg tgaaggcgcc tggtatgacc
    17341 cggatgcaaa acgtgtcgat aagggcggtt gtatcaacgt actgaccact caacgtccgt
    17401 ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag
    17461 gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc
    17521 aaaacctgcg agctggcttg taaagactac aaagatttga cgccagaagt cagtttccgc
    17581 cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg gcaccagaac
    17641 gtgtttgcct actatctgtc gatttcgtgc aaccactgcg aagatccggc ttgtactaaa
    17701 gtctgcccga gcggtgcgat gcataaacgc gaagatggtt ttgttgtggt cgatgaagat
    17761 gtatgcattg gctgccgtta ctgtcatatg gcttgcccgt atggcgcacc gcaatataac
    17821 gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa
    17881 aagccgatct gtgttgaatc ttgcccgctg cgggcgctgg atttcggccc tatcgacgag
    17941 ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc
    18001 aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga taccactggc
    18061 tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat
    18121 gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt atcgttctgg ctttggcgct
    18181 gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggctt
    18241 atgggtactg atgggcattg gctttatcgc ctctatgctc catcttggtt caccgatgcg
    18301 cgcctttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg
    18361 ttcgatcttt tttgctgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc
    18421 accggcattg cgtacgctat ggctggtggt gacgatggtt cttggcgtcg tctttgtctg
    18481 gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac
    18541 gccgatgggc tgggcgatgc gtctgctgcc agccatttct gtactggcac tggtggtgag
    18601 tggcgtgatg tcagtgatgc agggcacaga gctggcaact attcatagtt ctgtgcagca
    18661 ggccgcagcg ctggtgccgg actatggtgc gctgatgtcc tggcggatcg tgcttttggc
    18721 cgttgccctg tgcttgtgga ttgcaccaca gctaaaaggt tatcagcctg cggttccgct
    18781 actatccgtc tcattcattc tgcttctggc aggggaatta atcggtcgcg gcgtattcta
    18841 tggtttgcat atgaccgtgg ggatggccgt cgcaagctaa tcagtaacaa ccggggtttc
    18901 ggccccggtt ctcttttatt tctgcttcgt taacgtgtca taactggtca tcaaattacg
    18961 ataatcagga atatggttag agaacaacgt cgccagtcct tcaatatcat tacgccagtc
    19021 acggtgcagc tcgcaagcca cgccgaacca tgtcatcaat tgcgcaccag cttgcgacat
    19081 gcgatcccac gccgaatgcc gggtaatttc attaaaggtc cctgatgcat cagtcacgac
    19141 aaagacatca aacccttctt caatggctga aagcgccggg aatgccacgc aaacttcggt
    19201 taccacaccg gcaataatta actgtttttt acctgtcgct ttgactgctt ttacaaaatc
    19261 ttcgttatcc caggcgttaa tattgcccgg gcgagcaatg taaggtgcat cggggaattg
    19321 tgctttcagt tctagaacta atgggccgtt aggaccggtt tcaaaactgg tggtaagaat
    19381 agttggtaag ttgaaatact tagccaggtc acccagcgcc agcacgttat ttttaaactt
    19441 atcgggttcg atatcccgta caagggaaag taaaccagcc tggtgatcaa caagcaaaac
    19501 ggcagcatca tttttatcaa gacgaacata cggtttggtc atcctcttct cctttcgaga
    19561 gtcagtggta cgtctgaaat cggtcagacg cgatgcattg ctctgaaagc atagacggga
    19621 aatatgagtt tgctgtgacc atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt
    19681 tagatcaata aaaacaatca gatgagatta atcatctctt cagcattagc gttatgaatg
    19741 agtaatataa atcgataata ccgccgctgc gatatgcgcg tattttcacc cattgacaat
    19801 gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt
    19861 ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga
    19921 tcgcggtgtt aaatacactc gtgccgcttt ggctcgccca ggaacacatg tccacatggc
    19981 aggtaggcgt tgtcagctca tcctatttta ccggcaacct tgtcggtaca ttgctgacag
    20041 ggtatgtcat taaacgcatt ggctttaacc gcagctatta tctgtcctcc ttcatttttg
    20101 ctgctggctg tgccggcctt ggcctgatga ttggtttctg gagctggttg gcgtggcgtt
    20161 ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt tgagagcgcg ctgatgtgca
    20221 gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg
    20281 gaacgttttt aggccagtta ctggtcagca aagtctcaac cgagctgatg agcgtattgc
    20341 cgtgggttac aggtttgaca ttggcaggga tcttaccgct gttgtttacg cgtgtcctga
    20401 atcagcaggc tgaaaaccat gattcgacgt caattacggc aatgctaaaa ctccgtcagg
    20461 cgcggcttgg cgtgaatggc tgcattatct caggaatcgt tctgggatct ctatatggcc
    20521 tgatgcctct gtacctcaat cacaaagggg tgagcaatgc cagtattggt ttctggatgg
    20581 cggtactggt cagtgcgggt atcctcggac aatggccgat tggacgtctg gcggataagt
    20641 ttggtcgact gctggttttg cgtgttcagg tctttgtcgt cattctcggc agtattgcga
    20701 tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc
    20761 tatatccggt ggcgatggcc tgggcttgtg agaaagttga acatcatcaa ctggtggcga
    20821 tgaaccaagc cttactgttg agctataccg tgggaagtct gcttggcccg tcatttagcg
    20881 ctatgctaat gcagaatttc tccgataatt tattgtttat catgatcgcc agcgtatcgt
    20941 ttatctattt gctgatgctg cttcgcaacg ccggtcatac gccgaaaccc gttgctcacg
    21001 tgtaaatgaa ttcaagcaaa gtgtgaactt actgtttcac actctgcttt tttgtttctt
    21061 ctatctgact tgcttttttc caaattttat tcgtttaaaa ataaaatgtg cagcaggtta
    21121 taattttgca tttcgctatt tccgcacttc ttatttgccg cgcataatcc ctcgttttac
    21181 agatgcccct ttaattttgg cgaaggattt gtctatggct gggaatgttc agga
//
LOCUS       DADLDT020000069        20756 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000069 DADLDT020000000
VERSION     DADLDT020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.069"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..562
                     /locus_tag="JQH22_002481"
     CDS             <1..562
                     /locus_tag="JQH22_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFB2206767.1"
                     /translation="ALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQRAK
                     EARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQK
                     TLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSH
                     YELYDKPEAVQEVLAKVIPFFNTHLG"
     gene            580..1218
                     /locus_tag="JQH22_002482"
     CDS             580..1218
                     /locus_tag="JQH22_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFB2206768.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1509..2237
                     /gene="mpaA"
                     /locus_tag="JQH22_002483"
     CDS             1509..2237
                     /gene="mpaA"
                     /locus_tag="JQH22_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFB2206769.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2212..3171)
                     /gene="ycjG"
                     /locus_tag="JQH22_002484"
     CDS             complement(2212..3171)
                     /gene="ycjG"
                     /locus_tag="JQH22_002484"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFB2206770.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3290..3796
                     /gene="tpx"
                     /locus_tag="JQH22_002485"
     CDS             3290..3796
                     /gene="tpx"
                     /locus_tag="JQH22_002485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFB2206771.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3840..5381)
                     /gene="tyrR"
                     /locus_tag="JQH22_002486"
     CDS             complement(3840..5381)
                     /gene="tyrR"
                     /locus_tag="JQH22_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFB2206772.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5529..6590)
                     /locus_tag="JQH22_002487"
     CDS             complement(5529..6590)
                     /locus_tag="JQH22_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HFB2206773.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6587..7984)
                     /locus_tag="JQH22_002488"
     CDS             complement(6587..7984)
                     /locus_tag="JQH22_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFB2206774.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8140..9138
                     /locus_tag="JQH22_002489"
     CDS             8140..9138
                     /locus_tag="JQH22_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFB2206775.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9249..10121)
                     /gene="ompG"
                     /locus_tag="JQH22_002490"
                     /pseudo
     CDS             complement(9249..10121)
                     /gene="ompG"
                     /locus_tag="JQH22_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10194..11275)
                     /locus_tag="JQH22_002491"
                     /pseudo
     CDS             complement(10194..11275)
                     /locus_tag="JQH22_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11289..11948)
                     /gene="pgmB"
                     /locus_tag="JQH22_002492"
     CDS             complement(11289..11948)
                     /gene="pgmB"
                     /locus_tag="JQH22_002492"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFB2206776.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11945..14212)
                     /gene="ycjT"
                     /locus_tag="JQH22_002493"
     CDS             complement(11945..14212)
                     /gene="ycjT"
                     /locus_tag="JQH22_002493"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFB2206777.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14209..14832)
                     /locus_tag="JQH22_002494"
     CDS             complement(14209..14832)
                     /locus_tag="JQH22_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFB2206778.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14829..15251)
                     /locus_tag="JQH22_002495"
     CDS             complement(14829..15251)
                     /locus_tag="JQH22_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFB2206779.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15273..16048)
                     /locus_tag="JQH22_002496"
                     /pseudo
     CDS             complement(15273..16048)
                     /locus_tag="JQH22_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16066..17118)
                     /locus_tag="JQH22_002497"
     CDS             complement(16066..17118)
                     /locus_tag="JQH22_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFB2206780.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17149..17991)
                     /locus_tag="JQH22_002498"
     CDS             complement(17149..17991)
                     /locus_tag="JQH22_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFB2206781.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17978..18859)
                     /locus_tag="JQH22_002499"
     CDS             complement(17978..18859)
                     /locus_tag="JQH22_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFB2206782.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18880..20163)
                     /locus_tag="JQH22_002500"
                     /pseudo
     CDS             complement(18880..20163)
                     /locus_tag="JQH22_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20177..>20756)
                     /gene="ycjM"
                     /locus_tag="JQH22_002501"
     CDS             complement(20177..>20756)
                     /gene="ycjM"
                     /locus_tag="JQH22_002501"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFB2206783.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5064 a         5657 c         5049 g         4986 t
ORIGIN      
        1 cgcgctgaca gaaaaacgaa ttaaagctgt agtgagtatt acgggtgtta atatcgggcg
       61 tctttttcgg gaaggtttca gcaattatga tccgattggc gtattgaatg caatggcgtc
      121 acaacgagca aaagaggctc gtggtggaga attacagatc aacgaattac ttcccgccag
      181 cctggatgca gcaaaagccc atggactgac cgaacgcgat gtttatgaag caactgacta
      241 ctataaaaca ccacgcgggc aacaaccggg aggtgcgaca aaaatgttgt tctcacatgc
      301 acagaaaacg ctggcctggg atgccttcgc atttactgaa gtgttactga cccagccggt
      361 gatggtagta gttggtgaaa aagtcggtgc cttcggtgct tatcgtgatg gactggaagt
      421 ctatggccgg gcgatggttt cgcaggatcg tcagttagtt tcgttacccg atttctcaca
      481 ttatgaacta tatgacaaac cggaagcagt tcaggaggtg ctggcaaaag ttattccttt
      541 tttcaacacc catctgggat aagtttcatg agaaatatta tgaaaaatgt actgattctt
      601 ggtgctggcg gtcagatagc ccgccatgtg attaatcaac tggcagataa acaaactatt
      661 aaacaaactt tatttgcccg tcagccagca aaaatacaca aaccctatcc aactaatagc
      721 cagattatta tgggggatgt actgaatcat gcagcactga agcaggccat gcagggacag
      781 gatattgtgt atgccaacct tacgggggaa gatttagata ttcaggcaaa ttcagtgatc
      841 gctgcgatga aagcctgtga tgttaaacgt ctgatctttg ttctttccct cggtatttat
      901 gatgaagttc caggaaaatt tgtcgaatgg aataatgcag ttattggtga acccctaaaa
      961 ccattccgca gggctgcgga tgcaattgag gcttctggac tggaatatac aattcttcgc
     1021 cctgcctggc tgactgatga agatattatt gattatgagc ttacctctcg caatgaacca
     1081 ttcaaaggta cgatcgtatc acgtaagagt gtcgcagcat taattacaga tatcatcgat
     1141 aaacctgaga aacacattgg tgagaatata ggcatcaatc aacccgggac tgatggcgat
     1201 aagccattct tcatgtaaat caattatata gataccatcg tactgtacta tgtgcagtac
     1261 gatttattaa acttattaag gtgaactgat caatcagatg cgattaacat gtgtagaacc
     1321 agtaagaata aaatacgcca tgtatataac attacgacaa ttctaatatc tgcacatcaa
     1381 tgaaaatgca aacaacttcc ggcaaaaaag tgatacaaga tcgatgacat agcgttgaag
     1441 aaaaggattt gataatccgc aaaatttgca caacactgcc tgtaatactt cctgaaccag
     1501 agacgatcat gaccgtaacc cgctcacgcg ccgaacgcgg cgcatttccg cccggaacag
     1561 aacattacgg acgttcatta ttgggcgcgc cgttaatctg gtttcctgcc cctgcagcca
     1621 gccgcgaaag tggtttgatt ctggctggca ctcacggtga tgaaaactct tcggtcgtca
     1681 cgctctcctg tgctctgcgg actttgacac cttctttgcg ccgccatcat gtggtgctgt
     1741 gtgtgaatcc tgacggctgc cagctggggt tacgggccaa tgctaatggt gtggatttaa
     1801 acagaaactt tccggcggcg aactggaagg aaggtgaaac ggtttatcgc tggaacagcg
     1861 ccgctgaaga acgtgatgtc gtattactga caggtgacaa acctggctcc gaacctgaaa
     1921 cccaggcgct gtgccagctt atacaccgca ttcaacctgc ctgggtggtc tcattccacg
     1981 atccactggc ttgtattgaa gatcccagac acagcgaatt aggcgaatgg ctggcccagt
     2041 cgtttgaatt gcctctggta accagcgtcg gttatgaaac gcctggctct ttcggcagtt
     2101 ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt tccgcccatc tcctccgacg
     2161 aagccagcga aaaatatctc tttgcgatgg caaacttgct gcgctggcat cctaaagatg
     2221 caattcgccc gtcgtgaact gaagcgccgg ttccacatct accgccagcc aggtcggtcc
     2281 gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa gcggcgctaa tggcacgaga
     2341 ggtacacaac atgcagccca gcatcagact gaaaccttgt gcacgcgctt cagtcgccag
     2401 cgcttccgtc agaccaccgg ttttatcgag cttaatgtta accatctcat agcgcccttt
     2461 cagcgccttc agattgctac gagtatgaca actttcatca gcacaaatcg gcaacggatg
     2521 aataaaattc tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac
     2581 gcctaaatcc gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt
     2641 tgcatcaacg atcagcgtgg catcgggcac agcggtgcga attgccacca tccgctcact
     2701 gataagatgg ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga
     2761 tgcactattg gccatctgat caggcgtacc gatgacaacc gtctgtgcag tgataactgt
     2821 ctcgggaagc gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgccgcgag
     2881 atcccacaat gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa
     2941 ctcctcccgt gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac
     3001 cgaggcatca ctttctccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc
     3061 ctcttcttcc agttcaacca ccaccacgcg cgcttcactg cgacttcccc gggcaatcac
     3121 aaacggggta tgtaagggcc aggcttcctc aaatacctta acgattctca tctttactcc
     3181 ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt
     3241 agcataaact aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac
     3301 cgttcatttc cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa
     3361 agcgcagact tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc
     3421 gggtaaacgc aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc
     3481 agtacgtaag tttaaccaac tggcaactga gatcgacaac accgttgtgc tgtgtatctc
     3541 tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat
     3601 caccctctcc actttccgta acgctgaatt tctgcaagcc tacggtgtgg caattgctga
     3661 tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt
     3721 gattttcagc cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc
     3781 tgtactgaaa gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct
     3841 tactcttcgt tcttcttctg actcagacca tattcccgca acttattggc aatcgcggta
     3901 tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg
     3961 gttaataccg agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct
     4021 tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca aaatatcctg tggacgcagc
     4081 tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt tctttaactg ccgcacattt
     4141 cccggccacg cataacgtgt aagtacagta ttcaggtcag cggccagttt cggacgcggc
     4201 acgccctgct cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat aatgtcctgc
     4261 ggacagtcac gtagcggcgg cagattgagc gtcaacacgt tcagacgata atagagatct
     4321 tcacggaaca cgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc
     4381 cgcacatcga catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaaga
     4441 aaacgcagta atttcgcctg catccgtggt gacatttccc ctatttcatc caacagcacc
     4501 gaaccaccgt tcgcctgctc aaagaaccct ttcttccctt ccggagcatg accaaacagt
     4561 tcactctcga ccgcatcttc tggtatagac gcgcaattca gcgccaggta aggtttgccc
     4621 gctctggggc ttgcctgatg gcaggcgtag gcaaaaagat ctttacctgt acctgtgtca
     4681 cccgtaatca gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacgtgc
     4741 ttcattttcg ggctgacggc gacaatttgg ctgaaggcgc tgacgtcctg ggcggcgaca
     4801 ttttgcaact ggcggcccat acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg
     4861 acgtgttgat cattttcatc ctgaagataa acaggcgtaa tctccatcag gaaattctgc
     4921 ccattaataa cgacatgctc gttatgcgaa tcttgcggtt cgctttccag ccaacgtaaa
     4981 aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc
     5041 ccaaaaagct gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag
     5101 agcacaggtt caggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa
     5161 ggcatccacg ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc
     5221 agactgctga aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga
     5281 tcaatctcaa taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg
     5341 gtcagaccga gtcggtcttc acaaaagact tccagacgca tgggaacctt cacctgaaaa
     5401 aagaacaatt aatatgatga tatgacacta tttggatagc aggaaggttt ctgtcaacaa
     5461 tcattgacac aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg
     5521 atattgcatt atttttcgct gggcgttttg cctttttgca gagtttcttt cacctgaccg
     5581 ataagctgac gacggaaatc cccaaggcga ggtttgtcat cgtcaatcca cggcagcggg
     5641 cggcaaagct ccatagcttt aatcccgagt cgtaccgtca gaagtccagc accaattccc
     5701 tgagctgcgc gggtagacaa acgagcagcg agatcttgcg acatccagtc catccccact
     5761 tcgcgcacca gttcgctggc tccggcaaaa gcgatattca gcaataccag cttaaacaga
     5821 cgcaaacggc tgtaataccc cagttcaatg ccatacagcg tggcaatgcg attaattaaa
     5881 cgcagattgc gccaggcgat aaacgccata tcgaccaacg ccagcgggct gaccgcaatc
     5941 atcaacgttg attccgccgc cgaatggctg atttcgcgcc gcgcctgggc atctaaaact
     6001 ggctggacca aatgcgcata caggctgacc acttcacgat cgttctgcgt ttcatggatt
     6061 gaggcatacc agcgttgcag cgccggatgc gactgatcaa tacccgcctg ctgcgccagt
     6121 ttttcgcaaa atgcgcgacc tttgcccgtg ccgtggctat gcaataaatc acgcgcttcg
     6181 tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa
     6241 cctacgccag cgccgataat caatgcccca gcggcacatc cacccagcgc tacccagtcc
     6301 tgggtttgcc aggcattcat tgtccactgt ataccctgcc cgacaacgct tgcgccaaac
     6361 agagccagcc cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgcg
     6421 tccattaccg cttcgacttg cccctcttct tcctgcgctt cgtcgagcgt ggccggggca
     6481 aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgttcg
     6541 acctccagcg gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat
     6601 ctcctattaa aaattccagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat
     6661 ccatcacctg cgggcgaaac gcttcaaact ggaacccttg cttgtcccag aacgcctgac
     6721 cgggcaaacg cgcaggaact tcaccggggt aaacggtgag aggcgcacca tcgctgagcc
     6781 ggttaccgcg caatgccgga attttctcgc cgttgacgtc aataatgccg ctggtggtcg
     6841 cctgcaccga ggccagcccc aggcaatcca tactaatcct ttcaaacgcc gcattttgcc
     6901 aggcatcctg aatcagttgt tgcagtaatg aaaccatatt ggcgtgctga tcgaccgtta
     6961 catggtccgc tttcatcgcg gcaaacagca gtttgtcgat aaccggcgag aataagcggc
     7021 gaaacagcgt gcgctgcccg tagtggaaac tttgcatcag ctgcgtcagt gccagacgca
     7081 tatcattaaa tgcctgtggc ccactgttga gaggttgcag gcaatccacc agcacaatct
     7141 ggcggtcaaa gcgcaaaaaa tgattcttat agaacccctt caccactttc tcgcagtaat
     7201 aattaaaccg ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg
     7261 actcgtccca ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca
     7321 tatctcccgg caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt
     7381 ggtggagata atcggtccac acggcggcaa tgtctgccag ccggttttcg tcggcaggcg
     7441 ctagcgggtc aagtccttcg cacatcattc gccatttcgc cgaccattcg ccgcgctgac
     7501 cattgagtaa gcccgtcatc tggcgcgacc agcttaagta atcctgcgcc agcatcggca
     7561 ggtcgagcaa ccattcgccg ggataatcca caatttccag atacagcgtg gaggtatcct
     7621 taaagtggcg cagcagcgaa tcgttcgatt taaaacgtaa cgccaggcga atttcactga
     7681 cgccgcgcgt aggcgtcggc caggcgggag gatcaccata taactgcgcc agtccttcat
     7741 cataggtgaa gcgcggaatg ccaaagtcac gctggggaat gcgttttacg cccagcaagc
     7801 gctcttcacg caccgcgctt aacagcggca aacgtgctcc ggcatgaata ttgagcaact
     7861 gattaaccat cgcggtgata aacgctgttt tgccgctgcg gctaagtccg gttacagcga
     7921 ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt gagttcattt ttaagtcgct
     7981 tcattgctgt cctgtgctgc tctgaagacc agaaaaaaaa tcattcgtaa ggatatgaat
     8041 gtgggggcca ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata
     8101 agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac tatttatgat
     8161 attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa taagcaaact
     8221 aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag
     8281 ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat ggtgatttct
     8341 acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc
     8401 accgccaaag cggaagaaga aggtttcgac gtgatattac aaacgtcgca caatccggca
     8461 gaagatctgc aaaaatgcga aagcaaaatt aaacagaaaa tgattaaagg cattattatg
     8521 ctcagttcgc cagcggataa gtcatttttt gcccaactcg ataaatacga tattccggtg
     8581 gtggtaattg gcaaagttga aggtcaatat gcccatgttt attctgtcga taccgataat
     8641 tttggtgaca gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca aaatattgcc
     8701 tgcctgcatg ctccgcttga tgtccatgtt tcggttgatc gcgttaatgg ctataagcaa
     8761 agtctgtccg cgcataatat tgcagtgcgt gataaatgga ttgttgacgg cggttatacc
     8821 catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt gcctgaggca
     8881 gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc agagaaaaat
     8941 attgctattc cgcagcagtt agcggtggtg ggttatagta atgaaacgct gtcgtttatt
     9001 ttaacgcctg caccaggcgg catcgatgtt ccgacgcagg agttaggtca acaaagctgc
     9061 gagttattat tccgcttaat ttcgggaaaa ccgtcaccac agaatattac cgttgccacg
     9121 catatgacgc tgaaataaat accagctacc ttgcctgaat gccggatgcg atgctggcgc
     9181 atcttatccg gcctacattt atagatcgga taaagtactc gcgccgcatc cggcaattta
     9241 gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt atcgctgtcg
     9301 ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaacggaaag tccgttctgg
     9361 aaatcataac cgtaaaataa acccacacgg ttaaaatcat ggccttcacg ttcaatatca
     9421 tcctgccagt cccagttact ctagcgatcc agcccaatgc gcgtatacgg cgtcaccgag
     9481 tggttgccga gcgtcagcgg caaataggcg cgaatttctt gtgtggaaaa ctcaccgtta
     9541 ttgcggctgt cgtccatatt gaagccgcgc tcgagataat agttcactcg caaagcaacc
     9601 gtttcgttga aggtatattg cagacctgtt tccgtttcga cacgggtatc agcgtaaccg
     9661 gtcgtgttca aatcgttggc aaatttatac atcgacaacc aaccgttgaa acgtaaatca
     9721 tcagtcagtt tcacatccca gtctggcgcg attttccagc gctgcatatt cgccgtgtct
     9781 ttgcccggtt catcaacgta gtgataacca taattacgga aaccgccggt caggccgaaa
     9841 ctgaaatcat cgctttcgag gaactgataa tgcacctcca gctccgggcg atcaaacctc
     9901 gttccacgtt tacccgcgct ataatctacc ggcccttcct gataataggc cagagcaatt
     9961 ctccacggcc cgttggcggc attaaaatag actgaaggct ccgccagccc atccatatct
    10021 tcgccataac cctcgacgtt ttctatttcg tacatcgcac cgatattaaa gtgccagtcg
    10081 ctcttttcct cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc ggtacagggt
    10141 aataactttt tttgttcctt gtattatcct gggggaatgc cttccccctg tatttagcga
    10201 attgctattt ccgtttctgc atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg
    10261 atattttctc ctgcatgata atcatttaac gcaccagcac ggaccactaa ctcgtgcccc
    10321 caaccgtggt gtagagcata aattccgcac cggttaattc tgccacgcta attttggcgg
    10381 aaatgttatt ttcctcttgc gcgtccggat gaatatcttc cgggcgtatt cccatcacga
    10441 tgggcttatg caaactttcc tgtgttttca gaaccgctaa tttctcttcg agaatggtta
    10501 atttaagcgt ttccgtaacg aatttatcgc catcgatcgt gccgcgaata aaattcatcg
    10561 ctggtgatcc aataaatccg gcaacaaaca tattcgcggg ttggttataa acggttttcg
    10621 gcgcacctac ttgctgaaca atcccgtctt tcataatcac aatccgcgcc gccatggtca
    10681 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt gttcagtttc tgatgcagct
    10741 tgctgatctc tgcacgcatt tgtacgcgca gcttggcatc aaggttagag agcggttcat
    10801 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
    10861 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
    10921 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
    10981 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
    11041 cgatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
    11101 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgattta ccgcagcccg
    11161 acgggccgac aaatacgatg aactctttat cggcaatttc caggttgaag tccttcacca
    11221 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
    11281 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
    11341 gagggcaaca gtaattgcgc cccggttaag cccgcaccga tccccaccga gcgcataccg
    11401 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
    11461 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
    11521 agttgggaag catccgcgca gaaggtgaaa aactcgcgca gctccagcgc cgctaaaatc
    11581 gtcggcgcat tcagggagac ggaagccagc cctaccggaa tctgctgtgc acggagctct
    11641 gccagcaaat tgcgaatacc gggcagcaca gcattaaccg tcagctcgcg cagtgagtgg
    11701 acgtaaagca gatttttgcg atacgccagt tgcgcccgct cctgcgggtt aaagtcgccc
    11761 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttaga
    11821 gattcgttaa actgcgcatc aatactgatg ccaatttcag cggcaatctg ctgccacgcc
    11881 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat taccccttgc
    11941 agtttcattc atcctcctga tgcgtggtag cggtcccatt gaagggcaaa ataaaatcac
    12001 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
    12061 cgggcgcaga agttcgaatc gcaatacgct gcgcgtcgag agtgacctgt aattcgcagc
    12121 cctgccagaa cagagggaaa gacaactgtt gccactgctc aagtaacgcc ggatttagat
    12181 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
    12241 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
    12301 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcagcaa
    12361 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
    12421 gcagattggc aagacacgat gccgatgaga actgctctgg cagcatgtaa ttgagcatca
    12481 ccacatcagc ttgtttgagg atctgcatct cgttcacttc tgcgcgtgaa taatccagca
    12541 gaatggtttg cttccccgcc gccgctttgt atttcgccag attaatcgcc ggcttagtca
    12601 taaacgaatc atcctgcggc aaaacaccgt cgggctgaat ttctggcagc cgcagttctt
    12661 taaggaacat ttcggcacga tgaataaacg cgtcgtcact acagccaaac tggcgggcaa
    12721 tactcagcgc ctgctggacg ttgtaataca ccatatagct ggtgaaggcg ttattattga
    12781 catgttcggt atattcgtct ggcccaataa catcatgaat ttccagacgg tcgttaaccc
    12841 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
    12901 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
    12961 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
    13021 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
    13081 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
    13141 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatggaac ggcagcaaaa
    13201 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
    13261 caatactgct gcgctcgccg tgagccggcg tcatgatgcg cagatgataa agcgcataat
    13321 ctaacgcttg ctgatcgtgc gcatcgccgc catttaccgt gatacgacgt ttctgccacc
    13381 attgacgcca gttttctgtt gatgctgcaa gatgttggtc gtaactctgt tgcgcgcaca
    13441 tttcaagctg gcgaagcgac acgctgcccc actcgtctaa agcagcttgc ctgtcatccc
    13501 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgtac
    13561 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
    13621 cctggcagca acaactgatg gccacatcac tgcgcccatc ctgggtggtg tagatcccct
    13681 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
    13741 tggtttgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggca
    13801 taatagtgat ttccagcgca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
    13861 cgatggtgta acctgcgccg ttgctggtac gccagacaac gctacggcgt aattctccac
    13921 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
    13981 tggcaatctc catccccaag atatcaggca gattcaccag ttcgttgatt tcaccttttc
    14041 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
    14101 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
    14161 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
    14221 acgcgtccct gtttctgcgg attgatataa cgccgccacc agttgctgga tgatgtaccc
    14281 ctgctcggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
    14341 gctgcgcaaa tggcggttgt cgtctgccat ttcccgttgc atcagcgtca ttaattcacc
    14401 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccggctttat caccacaaaa
    14461 gctgacgttc ataatcaact gttcgcggat gttgagtgca aatgacgttt ccagccacag
    14521 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
    14581 cggatcccac tcgccaaatt gaccacagct cttttgcgtg ccgatctttt gaaaactatg
    14641 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
    14701 aatgccgatg tcgatcagca ggccaccacc ctgcagttct ttattggtaa agacacccca
    14761 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
    14821 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaacgatg gtgaaagtcg
    14881 taggccagca ccttgcccag tttgcgcgcg gtatcgcaca tttcccacgc ctgttctgtc
    14941 gtcatggcgg gcggtttttc gcacatcaca tggcagcctg cttccagtgc catcagggta
    15001 tgttcgtaat gaaaacggtt aggtgagccg acgctaacca catcaggttt caccgccagc
    15061 agcatggcct gcggatcgtc ccacacggat gcattcccgt atttttctgc cagcgcctgc
    15121 gcctgggaaa ggcggctgtc acagacagcc accagttcca gatcgttgcg ggtgcagtag
    15181 tacgaagcat gaaccttatc cgccacctgg cccgcgccta ttatcgcgac ccgcagcgga
    15241 gagcttgtca ttgcactttt cacaaagacc tcttagcagg tacgcaacca ggccaacgaa
    15301 tcacggtacg cctgggcagg atcttctgcc cggatacgcc cttcatacac cacataaccc
    15361 tgatagttat cggcgcgcag ctgttcaaac agcgcgtgga aatcgagggt gccactgccc
    15421 ggctggtaac gatggttatc ggcaatatgc acatgaccga gcaagtcacg gttgtcgtgc
    15481 agcgcctgcg ccaggttatc ttcttcgatg ttcatgtgat agaaatcgcc gataatctgc
    15541 acatgtttaa gattgttttc gacgatgtaa cgacgagcat cggcgagggt gttgatcata
    15601 tgatcctgat agcggtttaa cggttcgaga tacaccacgg ttccggtacg cgcggcgacc
    15661 tgttccagta cgcgcaggga atcactcacc agtttgcggt cgccgtccag gctacgcggc
    15721 gaggtcatcg gcggtaagcg gaaggtaaac atgccccacg ctgccggaaa ccccccactt
    15781 ctgccagcgc ctcgagaatg cgttcgatct gctttaagcc attaagacga cgctcttcga
    15841 taaagtcacc aatccagccg tcatagccgc cgcaggcggt ggtcactggt aaaccggttt
    15901 ctttgatcgc cgctttgact tcttcgatgt tgttgaccag cagtttgccg tcaatctcaa
    15961 aaccatcgaa gcccatctct ttgatataac gaaatctctc gcggatattt tccggaaaga
    16021 atgcctgatt ctgtgtgccg attttcattt tttcattcct ttgaattaaa acgtaacgcc
    16081 cattttgatg ctctgttccg gatgctgatc gacatactgc atgtagcttt ccgggctgtt
    16141 ggcaaaggtc actaccgggt cgattaaatc ttcgcaattg agataaccgt tcatcagcag
    16201 ttcccagcag gtttcttcaa tacgcttacg gctccagcgc ggataatccg ggttcggttc
    16261 gctgcacgcg cgggagaaaa caattttggc gttattgaaa tgcgcttcgc gtccgaggtt
    16321 aaaaccttca gcaaatggtt tggcaaacgc gacataggag atggtgccgc cgtaggccag
    16381 gccgcgcagc gccgattgca gcgcgtcggc gtaaccgctg gtttcgataa tcacatcagc
    16441 cccctgcttg ccggtcagcg ttttgatctc tttgcctacg tcagtgccaa tggggttaag
    16501 gcagaaatca gcaccgtggc gacgggcaat atcacagcga tgggcgatag gatcgacgcc
    16561 aattaccacc gatgcgccag cgcgtttcgc cagttggatg gcaatttgac cgatcgcgcc
    16621 aagccctacc accacgacaa aatcccctac gcgtacgttg gcatcgcgca cgccgctcat
    16681 ggcaaactgc gccgggtcgt aacagacggc gtttttccag gagctgcctt gcggcatttt
    16741 gcgcagcttg tagttattca ctgcgttaat gatgaccgtc tcggagagcg ggccgtagcc
    16801 gcatacgctg tcgcccaccg cgtagtctgt aacgtcgctg ccgcactcga taatgtcgcc
    16861 aaccaccatg ttgccaagct ggaatttgcc aaactcaatg ccgcgcggcg catctgccgg
    16921 acgcggggtg aacatctgcc actcgccgtt aaagtcttca tcgataaacg ggctggcggc
    16981 gcggaagtcg accacttccg ttccgtgttt cggtgcgccg aaacgcacgc ggatcttcac
    17041 ttcattggct aaaatcgccc gatcttcata ctcaaccagc gcagcaacac gcggtgctgt
    17101 ggctactaac tttttcatgt aaaactcctt gtaaagaaac tggccgcttt agcccttcac
    17161 gccaccggcg gtcaaaccac tcttaataaa acgttccgaa agcgcataca taatcaccac
    17221 cggcaatgcg gtcaccagcg aggccgccat catccgtccc cagatgtaat caggcgtact
    17281 gaacagcgcg ttcaggccca ctggtaaggt gaaattgctg gcgctggaga ggaaaatcga
    17341 cgcaaacaga tagtcgttcc acgccaccat aaagcagtag acgaacaccg atatcagccc
    17401 ggacatcgcc aggggcacgg tgatacggaa gatgatttgc agacggttga ggccatccat
    17461 catcgccgct tcttcgatct catcagggat ggtgtcgaag taacttttca gcataaacac
    17521 ggcggtgggc agggtttgcg tcaccatggt aatgatcagc gccatctcgg tgtcgtaaat
    17581 accaagcgcg gtgatgattt tgaacagagg caccaccagc aaaatgccag agaacatata
    17641 caccgtgtaa aagctggcgt tgatggtcat ccggccttta aagcgcaggc gggaaagcgc
    17701 gtaagctccg agaatgccga gaaataccgc caccacggag gaaaccaccg acaccaccat
    17761 gctgttacgg aagtagtcga caaacgggaa aatcatcggg ttaaaaatgt cgacgtaatg
    17821 ctccagcgtc cactgttgcg gcagcagcgt aggatgcagc gatatcgcct ctttcgcgct
    17881 tttgaacgag gtcatcagca tcacaaaaaa cggaaacagc gtgatgatga gaaacagcgc
    17941 cagcccgcag taaaaaccga tacgactgag cgtgcgttta tttgttgcca ttgaggttca
    18001 cccgtttcct ggtcagcaga atgacagcga aaataatgat gaagagcacc accgagatcg
    18061 ccgccgcttt ccctaagtca ttaaaggcaa aggcggtttt gtagagatag acaccaagaa
    18121 tatcgacctt ggttgtcagc agataaacat cagcgaacat gtagaacatc cagatggtgc
    18181 gcagggtcac caccgtcgcc aggaccggca taattgcggg cagcgtgacg atgcgaaacc
    18241 gctgccaggc attagcgcca tccatctctg ccgcttcgta cagcgatttg tcgatggtct
    18301 gcaaaatcgc cagaaacgag ataaaggcat acgggaagta gcgccagatg gcgaacagca
    18361 ccaccagcac aaagctactg cccggattat caaaccacag cggtgcctgc tcatagagat
    18421 gcagaagatc gacgccgagg tagttcacaa tgccgtagcc gttgttgaac atgtatttcc
    18481 aggcaaacac cagcgaaatg gacggcgtta cgtaggagag gatcaccagc gaacgcgcgg
    18541 ttttgcgcag gcggaactcg cggttgaaaa acatcgccac ggccagcccg agcacggtgc
    18601 tgcccgccac caccagcgcc gtataccaga ccgtcatcca cagcgagtgc cagaagccgg
    18661 gatcggagag aatgcgcaca tagttactca ccccaacaaa cgttgactcg atgttgggat
    18721 tgagcggcag acgtaaaaaa ctgatttcga tattcgacac catcggccac gccaccagac
    18781 cgcccagcag taataagctg ggcgcgagaa gcagcagcgc aaacggcata tcggaacgac
    18841 ctgaaaaaag cctgttcatt gcggattcct gtcctgggtt tagtgctgtt cgatcagttc
    18901 atccagcttt ttctggctcg cttgcagcgt gccagggaga tcggctttac ccacggtgac
    18961 gttatgcacc atggagctca ccacaccaga acccgtcaca tcgcccatgc gggtaaagtt
    19021 tttatcccct actgcgccaa agacctgaat atttggcagt tcaccgatta gctgattcgg
    19081 tagttcaccc agcgccttaa taacgtcgtt gtctttccag gtggcggtag tgacaaccgc
    19141 tttattcacc ggcagcgcag cacctggcga catcatcacc cagtcggcaa tgttgtccgc
    19201 ctgctccata aaggtgacaa atttttctgc tgcttcggtc ttttgtccgg cggtaatggt
    19261 cagcgaggtc aacatgccgt agaccgcaga gtttttctcg gttggcacca cgaaaccgac
    19321 gtttttcggg tcgccttctt taatcacagc cggaaggata taggtggagt aaatcgccat
    19381 cggcgcggtg ccgttcataa aggcgtcttt cacttccatg atgtcgttag aacccggcat
    19441 ggtgttggca gcaaggtcgc ggtaataggt cagcgcctgc atcatctctg gtgtatcaag
    19501 ggtgattttg ccttcggcgt taaagacgtt ggcctagttg gataacgcaa actgggagaa
    19561 ggattgttcc gtcagcacgc tttctgccgt aggcagcgca ataccgtatt ttttattcgc
    19621 cgggtcattc agtttctgtg caacgtccag cagctgttgc cagtttttcg gctcctccag
    19681 ccccgctttt gccagcacat ctttgcgata ccagatgtcg ccaatccagg cgctgacagg
    19741 aacaccggtc catgcgctac catcttcggt acgcacaata cgcagtacgc catcgtaaaa
    19801 cgcgccttca ccaacgttgc tgatgaccgt ggcaaccgct ttgcgatcga taagctgctc
    19861 tttatccata actttggcgt agtcatggct ggtttcgatc acttccggca gcgagccgct
    19921 acgtgaaagg gtgatgactt tggtgttatg agcgtcttct tccacgggca cttgtttaac
    19981 ggtgatacct gggttttctt tttcaaaacg ggcaatcaat ttgctgataa ctgcctgacg
    20041 ctcctgctcc accgaagaat gcataaattc gatggataca tgggttttat tttcttcttt
    20101 acagcctgaa atcagggcgc aggaaaccag tgctgataac agcacgattt ttgatttaat
    20161 catgagatgt tcctttttat ttaatccaca taacctgcca ggggcgcagc gttatttcat
    20221 tacccaatat atcaacttca ctaatcagat cccgaccatg cagatcagta atatttacat
    20281 gctgaatatt tttactgaca ttaaacaatc cagtcagaca attaccatca gcgttacttc
    20341 tttgaatacg cattacggat gaattaaccg tatcgatggt aaaattatta tccggatgaa
    20401 actcgttatg gctgcgacga agtgtaatta aacgcgacaa ctcatgatat accgcatgcc
    20461 ttaatgtagc ttcatcgttc agttctcgtg ttatctcttc actgtgatat tttttacggt
    20521 taatcgcacg gttatatccg agtttttcga cacctgcgta atcattacgc gagccaagaa
    20581 tactttgaat atatatcgcg ggtacaccgg ggaaacttaa caaaatcgca tgggcaagga
    20641 taaacctggt gcaacgttct tcatcgctac tctcgcggcg gcttaacgca ttcatatagg
    20701 tgacattcat ctcataggga ctgcgcgtgc cgtcgggatt atttttccag tttact
//
LOCUS       DADLDT020000070        20724 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000070 DADLDT020000000
VERSION     DADLDT020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.070"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            81..350
                     /locus_tag="JQH22_002502"
     CDS             81..350
                     /locus_tag="JQH22_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFB2206784.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="JQH22_002503"
     CDS             344..709
                     /locus_tag="JQH22_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFB2206785.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQH22_002504"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQH22_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFB2206786.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="JQH22_002505"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="JQH22_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFB2206787.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="JQH22_002506"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="JQH22_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFB2206788.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="JQH22_002507"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="JQH22_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFB2206789.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQH22_002508"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQH22_002508"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFB2206790.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQH22_002509"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQH22_002509"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFB2206791.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12404
                     /gene="malT"
                     /locus_tag="JQH22_002510"
                     /pseudo
     CDS             9674..12404
                     /gene="malT"
                     /locus_tag="JQH22_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="JQH22_002511"
     CDS             complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="JQH22_002511"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFB2206792.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="JQH22_002512"
     CDS             complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="JQH22_002512"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFB2206793.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14882..16480
                     /gene="rtcR"
                     /locus_tag="JQH22_002513"
     CDS             14882..16480
                     /gene="rtcR"
                     /locus_tag="JQH22_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFB2206794.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16462..17220)
                     /locus_tag="JQH22_002514"
     CDS             complement(16462..17220)
                     /locus_tag="JQH22_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFB2206795.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="JQH22_002515"
     CDS             complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="JQH22_002515"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFB2206796.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="JQH22_002516"
     CDS             complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="JQH22_002516"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFB2206797.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18628..20133
                     /gene="glpD"
                     /locus_tag="JQH22_002517"
     CDS             18628..20133
                     /gene="glpD"
                     /locus_tag="JQH22_002517"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFB2206798.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20402..20632)
                     /locus_tag="JQH22_002518"
                     /pseudo
     CDS             complement(20402..20632)
                     /locus_tag="JQH22_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4893 a         5698 c         5231 g         4902 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    10741 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    10801 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    10861 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    10921 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    10981 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    11041 atgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat caggatgaag
    11101 cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca
    11161 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    11221 tggcgctagt gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    11281 tatggagttt aattcagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    11341 gggaaacgca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa
    11401 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    11461 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    11521 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    11581 cccgtagcca gttgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    11641 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    11701 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    11761 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    11821 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    11881 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    11941 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    12001 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactt aatacgctgc
    12061 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    12121 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    12181 cagaactaat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    12241 actccggtta cagcaatgaa caaattgcgg gcgaactgga agtggcggca accaccatca
    12301 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gctgccgtgc
    12361 agcacgcgca gaaactgctg aagatgatgg gttatggggt gtgagtttag ccggataatg
    12421 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    12481 tctatcaaac caaaccgcac cggcaagaaa cgctccacca ccgcgatatt ggtcagcaga
    12541 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc
    12601 agttggtcgg cgagatattc ccccaccgcc gctgggcttg ccaggtagcg tttcacctct
    12661 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    12721 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    12781 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaaa tccccgccag tgtagcgatt
    12841 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    12901 atgttcccgc gctcgccaag ttgcaaggtg ctaaacgatg ccacgggcga gacttccgtt
    12961 gccaccacac cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    13021 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    13081 gacgggttat cggtgccgcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    13141 gcgggcagca ccgtttgcag caccagcgta caacttccgg cgctaccgat agcaaagcgg
    13201 taatcgccgc cgcgcacggt gccaggccgg aagaccagac gctgcgatcc cagctccgcg
    13261 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    13321 agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg ctggccggtt
    13381 atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcacca
    13441 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggtg
    13501 tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc
    13561 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    13621 atttggtctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    13681 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    13741 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcaccttta
    13801 cgcgtcacgt agatctcttc accaaagagc tgttcttttt gcacatagtt gtggtgacag
    13861 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    13921 aacgccgtta ccacattttc catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc
    13981 caggccacgg ctcttaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    14041 tcacgcgacg gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag
    14101 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgc
    14161 tccgactcat caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt
    14221 ttatagttgt tagtgttaag gaaacgcgga tatttttgcg ttaaccactg ataaccggcc
    14281 tccagctcag cccatttcgc atcgacgtta aaaggcgggt tttcccatgc gcctttatca
    14341 cgtttacaac gcccggtagt acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc
    14401 tccgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca
    14461 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    14521 ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    14581 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    14641 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    14701 ttctcttttc gttgtttgcg gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    14761 gcagattaat tttttaattt attgtttcta aaaagattat atctttatgt ccgtaaacgg
    14821 agatttcccg caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa
    14881 gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg
    14941 cagtcagcgc tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt
    15001 catcgatcga ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa
    15061 acgcgatatc gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca
    15121 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
    15181 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    15241 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc
    15301 aactccgcgc aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga
    15361 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga
    15421 tttcctcaaa tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    15481 cgaaaaagtg gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg
    15541 taagtcattt ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg
    15601 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt
    15661 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
    15721 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
    15781 aatgctgctg aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    15841 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    15901 aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    15961 tctacgccag cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc
    16021 cacactcact ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt
    16081 tgcaacatct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    16141 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat
    16201 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    16261 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    16321 ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca
    16381 gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    16441 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
    16501 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct
    16561 ccccatattg accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg
    16621 gcgcgagttc tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag
    16681 cgagccgtcg ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga
    16741 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc
    16801 ggcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt
    16861 ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt
    16921 gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat
    16981 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
    17041 ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
    17101 gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact
    17161 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    17221 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    17281 ctaaacccac ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    17341 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    17401 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
    17461 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca
    17521 gggcgctgat aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc
    17581 cgccgagata ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta
    17641 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    17701 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
    17761 cgatcatcat cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat
    17821 aacggcgata atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac
    17881 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    17941 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
    18001 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    18061 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    18121 gtacgccacc tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata
    18181 gaccacatcg tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg
    18241 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    18301 gtcgttggtt aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg
    18361 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    18421 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    18481 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    18541 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    18601 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    18661 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    18721 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    18781 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    18841 gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
    18901 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
    18961 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
    19021 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    19081 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    19141 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    19201 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    19261 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    19321 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
    19381 gataaacgca ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
    19441 gatgtggaat acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac
    19501 ctgctgaaag tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
    19561 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc
    19621 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    19681 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    19741 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    19801 gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
    19861 tcgctggcac gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat
    19921 gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg
    19981 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca
    20041 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    20101 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
    20161 tcaatctggc ctgagactga tgacaaacac aaaactgcct gatgcgctac gcttatcagg
    20221 cctacgtggt ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc
    20281 acgtcgcatc cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc
    20341 agggaattac cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc
    20401 ccactcgccc cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca
    20461 gaaggtaaat tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag
    20521 cccccagcat ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag
    20581 tcattaccag cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg
    20641 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
    20701 ggcgaaaaaa acgggaccct ttgg
//
LOCUS       DADLDT020000071        20714 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000071 DADLDT020000000
VERSION     DADLDT020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.071"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQH22_002519"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQH22_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFB2206799.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQH22_002520"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQH22_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFB2206800.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQH22_002521"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQH22_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFB2206801.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQH22_002522"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQH22_002522"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFB2206802.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8241
                     /gene="elyC"
                     /locus_tag="JQH22_002523"
                     /pseudo
     CDS             7461..8241
                     /gene="elyC"
                     /locus_tag="JQH22_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
     gene            complement(8218..9111)
                     /locus_tag="JQH22_002524"
     CDS             complement(8218..9111)
                     /locus_tag="JQH22_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFB2206803.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="JQH22_002525"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="JQH22_002525"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFB2206804.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="JQH22_002526"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="JQH22_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFB2206805.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="JQH22_002527"
     CDS             complement(10242..11474)
                     /locus_tag="JQH22_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HFB2206806.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="JQH22_002528"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="JQH22_002528"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFB2206807.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="JQH22_002529"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="JQH22_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFB2206808.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="JQH22_002530"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="JQH22_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="JQH22_002531"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="JQH22_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFB2206809.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="JQH22_002532"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="JQH22_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFB2206810.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="JQH22_002533"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="JQH22_002533"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFB2206811.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="JQH22_002534"
     CDS             complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="JQH22_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFB2206812.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4983 a         5726 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgcaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtgc
//
LOCUS       DADLDT020000072        20649 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000072 DADLDT020000000
VERSION     DADLDT020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20649
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.072"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            223..789
                     /gene="yqaB"
                     /locus_tag="JQH22_002535"
     CDS             223..789
                     /gene="yqaB"
                     /locus_tag="JQH22_002535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFB2206813.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            786..1199
                     /locus_tag="JQH22_002536"
     CDS             786..1199
                     /locus_tag="JQH22_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2206814.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFCALVKRYAMLQLQQRPFRA"
     gene            1286..2842
                     /gene="gshA"
                     /locus_tag="JQH22_002537"
     CDS             1286..2842
                     /gene="gshA"
                     /locus_tag="JQH22_002537"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFB2206815.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2992..3507
                     /gene="luxS"
                     /locus_tag="JQH22_002538"
     CDS             2992..3507
                     /gene="luxS"
                     /locus_tag="JQH22_002538"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFB2206816.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3571..5109)
                     /gene="emrB"
                     /locus_tag="JQH22_002539"
     CDS             complement(3571..5109)
                     /gene="emrB"
                     /locus_tag="JQH22_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFB2206817.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5126..6298)
                     /gene="emrA"
                     /locus_tag="JQH22_002540"
                     /pseudo
     CDS             complement(5126..6298)
                     /gene="emrA"
                     /locus_tag="JQH22_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(6425..6955)
                     /gene="emrR"
                     /locus_tag="JQH22_002541"
     CDS             complement(6425..6955)
                     /gene="emrR"
                     /locus_tag="JQH22_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFB2206818.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7046..7381)
                     /gene="ygaH"
                     /locus_tag="JQH22_002542"
     CDS             complement(7046..7381)
                     /gene="ygaH"
                     /locus_tag="JQH22_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFB2206819.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7371..8108)
                     /gene="ygaZ"
                     /locus_tag="JQH22_002543"
     CDS             complement(7371..8108)
                     /gene="ygaZ"
                     /locus_tag="JQH22_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFB2206820.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8232..9411)
                     /locus_tag="JQH22_002544"
                     /pseudo
     CDS             complement(8232..9411)
                     /locus_tag="JQH22_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9603..10595)
                     /gene="proX"
                     /locus_tag="JQH22_002545"
     CDS             complement(9603..10595)
                     /gene="proX"
                     /locus_tag="JQH22_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFB2206821.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10653..11717)
                     /gene="proW"
                     /locus_tag="JQH22_002546"
     CDS             complement(10653..11717)
                     /gene="proW"
                     /locus_tag="JQH22_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFB2206822.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11710..12912)
                     /gene="proV"
                     /locus_tag="JQH22_002547"
     CDS             complement(11710..12912)
                     /gene="proV"
                     /locus_tag="JQH22_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFB2206823.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13267..14226)
                     /gene="nrdF"
                     /locus_tag="JQH22_002548"
     CDS             complement(13267..14226)
                     /gene="nrdF"
                     /locus_tag="JQH22_002548"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFB2206824.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14236..16380)
                     /gene="nrdE"
                     /locus_tag="JQH22_002549"
     CDS             complement(14236..16380)
                     /gene="nrdE"
                     /locus_tag="JQH22_002549"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFB2206825.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16353..16763)
                     /gene="nrdI"
                     /locus_tag="JQH22_002550"
     CDS             complement(16353..16763)
                     /gene="nrdI"
                     /locus_tag="JQH22_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFB2206826.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16760..17005)
                     /gene="nrdH"
                     /locus_tag="JQH22_002551"
     CDS             complement(16760..17005)
                     /gene="nrdH"
                     /locus_tag="JQH22_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFB2206827.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17352..17582)
                     /locus_tag="JQH22_002552"
     CDS             complement(17352..17582)
                     /locus_tag="JQH22_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206828.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            17734..18078
                     /locus_tag="JQH22_002553"
     CDS             17734..18078
                     /locus_tag="JQH22_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HFB2206829.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18115..18564)
                     /gene="alaE"
                     /locus_tag="JQH22_002554"
     CDS             complement(18115..18564)
                     /gene="alaE"
                     /locus_tag="JQH22_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFB2206830.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            19232..19636
                     /gene="stpA"
                     /locus_tag="JQH22_002555"
     CDS             19232..19636
                     /gene="stpA"
                     /locus_tag="JQH22_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFB2206831.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(19683..20207)
                     /gene="ygaP"
                     /locus_tag="JQH22_002556"
     CDS             complement(19683..20207)
                     /gene="ygaP"
                     /locus_tag="JQH22_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFB2206832.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(20217..20516)
                     /locus_tag="JQH22_002557"
     CDS             complement(20217..20516)
                     /locus_tag="JQH22_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2206833.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         5229 a         5162 c         5282 g         4976 t
ORIGIN      
        1 atccgtagct cagctggata gagtactcgg ctacgaaccg agcggtcgga ggttcgaatc
       61 ctcccggatg caccatattc tccaaaaaat ttccttcaaa aatatctcat ttctaaataa
      121 ctactttatc catttcctga attttcttct ccttcgccat cagcgacaaa atcaagcaat
      181 tacgctaaag tagcgtcata tttttcgact tgcgagaaga ccatgtacga gcgttatgca
      241 ggtttaattt ttgatatgga tggcacaatc ctggatacgg agcctacgca ccgtaaagcg
      301 tggcgcgaag tattagggca ctacggtctt cagtacgata ttcaggcgat gattgcgctt
      361 aatggatcgc ccacctggcg tattgctcag gcaattattg agctgaatca ggccgatctc
      421 gacccgcatg cgttagcgcg tgaaaaaaca gaagcagtaa gaagtatgct gctggatagt
      481 gtcgagccgc ttcctcttgt tgaagtggtg aaaagttggc atggtcgtcg cccaatggct
      541 gtaggaacgg ggagtgaaag cgccatcgct gaggcattgc tggcgcacct gggattacgc
      601 cgttattttg acgccgtcgt cgctgctgat cacgtcaaac accataaacc cgcgccagac
      661 acatttttgt tgtgcgcgca gcgtatgggc gtgcaaccga cgcagtgtgt ggtctttgaa
      721 gatgccgatt tcggtattca ggcggcccgt gcagcaggca tggacgctgt ggatgttcgc
      781 ttgctgtgag tgaagcgtta tcgcttttct cgttgtttgc cagtagtttt ctcagcgcta
      841 cactattacc cggcaactcc gaagtcgtgc tggtggcaat gttgctttcc gggatcagtc
      901 atccctgggt tttagtttta acagcaacaa tgggtaatag ccttggaggg ttaactaacg
      961 ttatccttgg gcgtttcttt ccattgcgta aaacatcgcg ctggcaagag aaagctaccg
     1021 gctggctgaa acgctatggt gcagtcacgc tattattaag ctggatgccc gtggttggcg
     1081 atttactgtg tctgttagcg ggatggatgc gcatctcctg ggggccagta atcttttttt
     1141 gtgccttggt aaagcgttac gctatgttgc agttgcagca gcgaccgttc agggcatgat
     1201 gtggtggcac taattgtagg cctgcacata tggtcaccat tacagttatg ctaattaaaa
     1261 cgattttgac aggcgggagg tcaatttgat cccggacgta tcacaggcgc tggcctggct
     1321 ggaaaaacat cctcaggcgt taaaggggat acagcgtggg ctggagcgcg aaactttgcg
     1381 tgttaatgct gatggcacac tggcaacaac aggtcatcct gaagcattag gttccgcact
     1441 gacgcacaaa tggattacta ccgattttgc ggaagcattg ctggaattca ttacaccagt
     1501 ggatggtgat attgaacata tgctgacctt tatgcgcgat ctgcatcgtt atacggcgcg
     1561 caatatgggc gatgagcgga tgtggccgtt aagtatgcca tgctacatcg cagaaggtca
     1621 ggacatcgaa ctggcacagt acggcacttc taacaccgga cgctttaaaa cgctgtatcg
     1681 tgaagggctg aaaaatcgct acggcgcgct gatgcaaacc atttccggcg tgcactacaa
     1741 tttctctttg ccaatggcat tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc
     1801 caaagagaaa atttctgcgg gctatttccg cgttatccgc aattactatc gtttcggttg
     1861 ggtcattcct tatctgtttg gtgcatctcc ggcgatttgt tcttctttcc tgcaaggaaa
     1921 accaacgtcg ctgccgtttg agaaaaccga gtgcggtatg tattacctgc cgtatgcgac
     1981 ctctcttcgt ttgagcgatc tcggctatac caataaatcg caaagcaatc ttggtattac
     2041 cttcaacgat ctttacgagt acgtagcggg ccttaaacag gcaatcaaaa cgccatcgga
     2101 agagtacgcg aagattggta ttgagaaaga cggtaagagg ctgcaaatca atagcaacgt
     2161 gctgcagatt gaaaacgaac tgtatgcgcc gattcgtcca aaacgcgtta cccgcagcgg
     2221 cgagtcacct tctgatgcgc tgttacgtgg cggcattgaa tatattgaag tgcgttcgct
     2281 ggacatcaac ccgttctcgc cgattggtgt agatgaacag caggtgcgat tcctcgacct
     2341 gtttatggtc tggtgtgcgc tggctgatgc accggaaatg agcagtagcg aacttgcctg
     2401 tacgcgcgtt aactggaacc gggtgatcct tgaaggtcgc aaaccgggcc tgacgctggg
     2461 tatcggctgc gaaaccgcgc agttcccgtt actgcaggtg ggtaaagatc tgttccgcga
     2521 tctgaaacgc gtcgcgcaaa cgctggatag cattaacggc ggcgaagcgt atcagaaagt
     2581 gtgtgatgaa ctggttgcct gcttcgataa tcccgatctg actttctctg cccgtatctt
     2641 aaggtctatg attgatactg gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg
     2701 taatctgctg cgtgaagagc cgctggaaat tctgcgcgaa gaggattttg tagccgagcg
     2761 cgaggcatct gaacgccgtc agcaggaaat ggaaaccgct gataccgaac cgtttgcggt
     2821 gtggctggaa aaacacgcct gacagaaaag aaaaaggcca ctcgggagtg gccaaaattt
     2881 catctctgaa ttcagggatg atgataacaa atgcgcgtct ttcatatact cagactcgcc
     2941 tgggaagaaa gagttcagaa aatttttaaa aaaattaccg gaggtggcta aatgccgttg
     3001 ttagatagct tcacagtcga tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa
     3061 acaatgaaca ccccgcatgg cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg
     3121 aacaaagaag tgatgccaga aagagggatc cataccctgg agcacctgtt tgctggtttt
     3181 atgcgtaacc atcttaacgg taatggtgta gagattatcg atatctcgcc aatgggctgc
     3241 cgcaccggtt tttatatgag tctgattggt acgccagatg agcagcgtgt tgctgatgcc
     3301 tggaaagcgg caatggaaga cgtgctgaaa gtgcaggatc agaatcagat cccggaactg
     3361 aacgtctacc agtgtggcac ttaccagatg cactcgttgc aggaagcgca ggatattgcg
     3421 cgtagcattc tggaacgtga ggtacgcatc aacagcaacg aagaactggc actgccgaaa
     3481 gagaagttgc aggaactgca catctagtca gtaaactatc ttcacaatta attgaaaaaa
     3541 gccagttcaa atgaactggc ttagttgtac ttagtgcgca ccgcctccgc cgccacctgc
     3601 gccaaatggc ggtttagcaa accacaccag ccccagcagt acgaggaata tcccggctga
     3661 catccagaag atctcattgg cggaaataat cagcccctga ttggtgatct gctgggcaat
     3721 ccagcctgat gcctgctgtt gcgtcatccc aagcccttcc agttgactgt acatcgcctg
     3781 ggcattcggg ttgaacgggt ttaccgactc agtcaactgc gcatggtgca tcgactcgcg
     3841 gttggtccac atggtcgtgg ttatcgacgt gccgatagac cccgccagcg ttcgcgtaaa
     3901 gttagagagg ctcgatgccg ccgccagtcg ttccggtggc aaaccagaca gcgtaatggt
     3961 ggtcagcggc ataaagaagc agaccaccgc aaacccctgg ataaactgcg gccaggccga
     4021 cgcgcaaaaa tccatacctg gttcaaaggt ataggcacgc cagtagaagc agacggcata
     4081 cataataaag ctgaaggtta ccagccgccg catatccagt ttatgcgcga agcggccgat
     4141 aatcggcgac agaatcaccg gaataatccc taccggcgca gaggccaaac ctgcccaggt
     4201 cgccgtgtaa ccgtagacct cctgcaacaa ctgcggcagc agaacaatag cgtcgaagta
     4261 gagcatatac gcgaggctga tacacaagca gccgatggtg aagttgcgcg acttaaacaa
     4321 cgacagatcg actatcgggt tatcgtcggt cagctcccag acaatcagga agcagatagc
     4381 caccaccgcc accacggtaa gaatgataat ttcctgtgat gaaaaacagt ccagctcttt
     4441 accgcggtcg agcataatct gcaggctgcc gataccaata accagcagtg ccagccccac
     4501 ggcatcaatc cgccgccgtt cggtgcgggt ttcacgtccg cgcagggttt gcagtgtcat
     4561 caacaccacc gccacgccaa tcggcacgtt gatgaagaat atccagcccc agtggtaatt
     4621 atcgctgata taaccgccga ggatcgggcc gcaaattggc gcgacaatca ccgtcatcga
     4681 ccacaacgcc agcgcgatcg agcgtttggc tggcgggtag ttattcagca atagactttg
     4741 cgaaagcggg atcaacggcc cggcgacaat cccctgaatc acgcggaaga agatcagcat
     4801 attcaggctg ctggagacac cacacgccca agacgcaata gcaaaggcga tggtggacca
     4861 aaggaacagt ttcacttccc cgacgcgctt tgccagccag ccggtaagcg ggatcgagat
     4921 ggcattcgcc accccgaaag aagtgattac ccacgttcct tggctgagcg atgagcccag
     4981 attcccggcg atagtgggga tcgccacgtt agcaatggtg gagttcagca cctgcatgaa
     5041 tgtcgccagt gacagcgcaa tcgtcataat gaccagttgc gcgccttcca gcggtttttg
     5101 ctgttgcatc acacgcacct ctggattagc cagcgttagc ttttacgata tcgtcgatca
     5161 gtttattgac aggtgccagg ctgatttcac gcgcggtact taccgcgacc ggtgttgaac
     5221 gtactttatt tgccagtacc tgaccgtcac ggttagtggt attgacgctc accagcgtgg
     5281 acaaaccaat gcgcagcggg tattgctcca gctgtttctg gtccagttcg atacgcacag
     5341 gtagacgctg aacgactttg atccagttac cggtcgcatt ttgcgccgga agcagtgaga
     5401 acgcgctacc tgtgcccata tccagaccaa ccactttacc ggtgtatttc acatcatcgc
     5461 cgtaaatatc ggtggtgatg gtgaccggct gaccgatgcg catattggca atctgcgtct
     5521 ctttaaagtt ggcatccacc cacatattgg tggctggaac gaccgccatc agcggcgtcg
     5581 ttgggctaat ttgcgcaccc ggttgcacgg cacgacggga aacataaccg gtcatcggac
     5641 tgacaatacg ggtacgctcc agcgccagct aggcgttacg tacttcggtg gcagcctgtt
     5701 gcacggcagg ctgatcttcc agtttagtcc ccagaatcat cgcctgattg gcattgtatt
     5761 gttgaatagc gacgtccagt tgcgcctggg cactggtgac ggcgtcgcgg gcgtgttgca
     5821 gctcttcgcg accaatcagg ttggcattgc ccagcggcac acggcggttg tagtcgcttt
     5881 gtgctttcgc gagggcgatt ttttgcacct caatattcgc ctgcaactgt ttgctgttaa
     5941 tcatcagctg gtgggtttgg cgaacgctgg aagccagtgc agttttggct ttttcaaacg
     6001 cctggcgagc atctgtcggg tcgagagtga ccagaacatc gccttctttt acaaaatcgg
     6061 tgttatcggc ccagactttc gtcacgctgc cagacacctg agacataatt tgaatctgat
     6121 tccctgccac gtatgcgtca tcggtttctt cgaaatgacg cagtaccaaa aaccaataaa
     6181 tccctatcgc tacggcaata attataaaga gcaaggtgag aaggaggagc agacgcttac
     6241 gtttgccgct cttctttacc ggttgctgcg gggtttgagt ctccgcattt gcgctcatat
     6301 tgttctccac gatcttctta actcatcggc tgagccgacc tgcttgccaa aaaggccagc
     6361 atgttgcgat gctggccggt cattttttct tttataaatc tggatttttg agcgagatga
     6421 cgcgttagct catcgcttcg agaaccacac cgtcttgttc catctggtcg agacgggaga
     6481 gcaatttgcg ggtgatttgc tcgagctgat ctttttctgt tgtgctgagc gcggaccaga
     6541 gttgatgcag gcagttatgc tgcggtggta aaacctcgcg caaaaactcg tgaccttttt
     6601 ccgttaattg cagatgcagg cagcggcgat cgttatcgct tttacgacgt tcgatccaac
     6661 cgcgtttttc cagttcatcg gcaatacgcg tcgcgttggt acgggatgat ccaagagcac
     6721 aacttaattc agaaggctga atactgtggt tttcctgaga ctccagcgta atcaacgcca
     6781 taaacaacgt ctcgttaatc ccctgagcct tcagcatttt attgcggttc tccagcagct
     6841 tgctttgcat gtgcatgcaa agacgagtca gaaggatctc ctgataagga aaatcttcgt
     6901 ggcggctggc gcgaaatttt agcatttgtt caatgggcgt aaacgaacta tccatttggg
     6961 tatgacctca ttaatttcag ccgatatagt aacgacagtg acaaataaag taaatgtatt
     7021 gttttagaaa aatgattctt gtgggttata taatcgccat cactttccag gcgagcccat
     7081 aggccagcgc actaagcagt gttgggatga taatgctgcg tgttttatag aaactggcac
     7141 ccagtaccgc gaagccgacc agcgtgggca cgaaacggcg tgtatcgtgc atcacttctg
     7201 gtgcggtaga gacaaccagc agagcgcata tcgaggcgat gccaatggtg tcgagcaaaa
     7261 tacctaccgc gccacgtttg gttgggcggg cattacccac acgcaggcgc agcggcaaat
     7321 agcggaagca ataattcgcc gcgccaacca gtaacccaag cagcagaacc tcatagctca
     7381 tcgggcgctc cttgccagaa tgcctggatt aacgcagtga ggcagccaca gacaatgcct
     7441 gccagaatgg cgacgggaat agaaaatagc gttacgcctg caagggcacc aactaaagct
     7501 gcggtaacgc aaagagattg tttgcgctgg aaagaggcga gcaggaaact cataaagagt
     7561 gccggaagca taaaacctaa tgcagcttca acggcgggat aaccttgcag caagccgctg
     7621 ccggagaatg cccctattac cgtaccaaat acccacgatg accatgaact gaaggcaatg
     7681 ccgatcatcc agttctcgct ccagcggcga ttattgcgta ccagttttgc ggtggcggcg
     7741 gcaaaaacct catccgtcag gccaaacgcc catagggcgg ttttcgattt ttgcagacgc
     7801 tgaataatac ggctacgcag tgacgggcca tacaaaacat ggcgaacatc cattgccatg
     7861 acgctcagtg cagcaaccca caaactactc ccggctgcca gcatcgcggt aatgacgaac
     7921 tggctcgcgc ctgcataaat gatgcaggag aaaaaaacgc tttcgagagg agagaatccc
     7981 agacgggtcg cattcagacc gaacgcaaag gccaccggaa tataactaat aacaatcggt
     8041 aaactgtctt tgcatccttc catgaaggtc gccgaaccag gagcaggctg tggagtaggg
     8101 ctttccatag agtgtacgct taacgattgt taatattata aatagactga atgaatatct
     8161 taaccttatc agactgatgg gcttcttaac acccttataa gtgtaaagcc acgaaaacgg
     8221 ttgctgattg cttaatttgc ggcttcctga cgatgaaaac ctcgccacca gaccagtaag
     8281 ttaaccaggg caatcgtcgc gccagccaga caaacgccag cccaaccgcc atgttgccag
     8341 gctgaggctg aaattagcga accggcggca ccgccaataa agtagctggt catgtaacct
     8401 gcggtcaggc gattacgcgc atcaggatgt attcgataaa ttaccgtctg gttagtgata
     8461 tgcacgccct gtacggtgag gtccagcacc agaatgccga taatcaacgc cagtacggaa
     8521 gtgtgaccaa accagattgc cagccatgaa agtaacagca gaagcagacc gaaagttgtg
     8581 gtgcgatttg cccttgtcgg caaaaccgcc cgccggacga gcgcccaacg ctccggcagc
     8641 tcccgcaagc ccaaacagac caatgtcacc atcgctgtag ttaaaaggtg gagcggcaag
     8701 caaaaaggcc attgaggtcc agagaatgct gaagttggca aaggtcatgc agcccagcaa
     8761 cgcgcgggtg cgcaggatct tatcgctgat gaacatgctg aaaacggaac ccaacaactg
     8821 tgggtagttg aggtgggttt ctgatttcat ttgtggcaga ccacgccata atgccagcgc
     8881 catcagtgcc attaacaccg aagcaaccca aaagacggtg cgccagccgc cgaggtttgc
     8941 cagtaacccg gcaacggttc gtgccagcaa tatccccaac agcagaccgc tcataatggt
     9001 gccaaccact ttgccgcgtt tatccggtga agccagcgtc gctgccagcg gaaccagaat
     9061 ttgtgccacg actgagaata aaccggttaa tgccgtaccg aggatcatca tcgccagtga
     9121 ctgactgctg gcagtgatta acataccgcc cgctgccagt aaggtcatcg agacaatcag
     9181 gcggcggcgt tcaaacatat caccgagggg aacaagaaac agtagacctg cggcatagcc
     9241 caactgcgcg gcggtaacaa taaagcctgc cgaactggcg gaaagggaaa agttacgcgc
     9301 gatggtgtcg agcaatggct gggcgtaata gttactggct accgccagac cggtggcgat
     9361 agacatcagc acgatcagcg ccgggctaag ctcatgatta ggtttagtca ttatttttgt
     9421 tcatcaggtg atgattaagc tggaaatcaa taatagcgga tgttgatgaa tatgggatga
     9481 aagatagctt gttgaaatgt gcgatgtggt ttgtaggcat gataggacgc gtcagcgtcg
     9541 catcaggcat tgtgtacaga gcggcggatg aggcgaaaac gccttatccg cccgaataaa
     9601 aattacttct gcgctgccag cgcctcattc acccagccat cgaactgctg ctggtgggct
     9661 ttgatccagc catcaacgtg gccctgaata tcgccttctg aggctttgcc gtcatgcata
     9721 atggcgttct gggcgttaat atcggccact ggcaactgca taatggcaaa cagtttggct
     9781 gccgccggat ttttctcggt ccaggctttg ttggcaacga tatgcatggt gctgaccggg
     9841 aagccgtaat tcgcgccatt cggcagtttg gtatcggcat ttttatcgcc cggcagtgcg
     9901 gagaacggca cctgcaacca gaccacatct ttccctggct tcagctcatt actcacccag
     9961 tacggcgtcc aggtgtaata aaacaccggt ttgccctctt tgtagcgact gatggtgtcg
    10021 gccatcatcg ctgcgtagtt cccctgatta tgagtcacgg tgttggtcag ttcatacgcg
    10081 gcaagctggt ggttgatcgc accttcgcag ccccagccag ggttacaacc ggttaaatcc
    10141 gctttaccgt cgccgttggt atcgaacagt ttggcgatct tcggatcttt cagttgtgcg
    10201 atgttggtga ttttgtattg gtcggcggtt ttcttatcga tcaggtaacc ctgtgctgcg
    10261 ccgttaacaa ataccccttc acgataaaat ttcttatcgc caccggcagc ttcgtacatg
    10321 ttgtcatgga gtggcgtcca gttcacggcg gtgaaggttg catcgccgga agcaagcgag
    10381 gtgtagccaa cgttgtaatc cacttcgctg ggtttgttga cggtataacc taatttctcc
    10441 agcgcacgac tgaccagcag cgtctggaag gtttcttcag tgatggtgct ctgaactgga
    10501 ttaacagtaa tgcctttgcc cggcagatcg gcagcaaaag tttgtgtaga gataagcgtg
    10561 gcaaacgctg tcgcaaaaag tacgctatgt cgcattgtta ttcctttttt tggcagggtg
    10621 ataatgcccg gcgccaccgg gcaagtgcag agttacttaa tgaatgggcg ggtcagcaga
    10681 ccaacagggc cagtggtgta ccagcgacgg ttgccgcgac tgcgtgagtc gcgcccaacg
    10741 gcctgcgtca ggcgatcgag gataatggcg aggatcacaa tcccgacgcc gccaacggtg
    10801 gcaagcccca tatccagacg accgataccg cgaagtacca tctgacccag cccgccgacg
    10861 gcaatcatcg aggcaatgac caccatagaa agggccagca tcagcgtctg gttaacgccc
    10921 gccataatgg tcggcatcgc cagcggtaac tgaactttga acagcatctg gcgcgggctg
    10981 gcaccgaatg agcgcgaggc ttcaatcaga tccgccggaa cctggttaat ccccagaatg
    11041 gtcagacgga taatcggcgg cagcgcaaag atgatcgtca ccaccacgcc cggcacgtta
    11101 ccgataccaa atagcatgac gattggcacc agataaacaa acgctggcgt ggtctgcatg
    11161 gcatcaagca gtggacgaat aattttcgcc gctcgcggac ttctcgccag ccatatcccc
    11221 aacggcaaac cgatgacgat acagaacagc agggcagtta acaccagcgc cagagtcacc
    11281 attgcctgcg accaggcacc gattgcgccg atggcaatca gcgagaccag cgtcgccacg
    11341 cccattccga ccccggaaat ctgccaggtg atgagagcga aaacgataat cgccactggc
    11401 gctggcatac ccagcagcaa ttgctggaaa ccgttgagga tataatcaac cggaacgcgc
    11461 acgccctgga agacgggacg gaaatgggta acaacccagt cgatcccttc agtgacccaa
    11521 ctgtcgagcg ggatcagcgt tttatggaac ggatcgagaa tattaaaatg ctcgacgttt
    11581 ggcgcaggcg tactggtcag ccagtcagca ccaccgccgt cagccggtgc agtcgtcggt
    11641 gtaccccagg cgtctgcgga ttgcgcggca ctgtccgccg ctggcgtggt atcccacgga
    11701 ttattttgat cagccattat ttaccccctc acgatctaaa gcgcgcagca gcattccttt
    11761 cgaaatgatg ccgacatact gttggtcctc gtcgaccacg ggcaccgcac agggtgcctg
    11821 tccgacatga gagagcaact cgctaagagg cgtttgtgca tcgactgcta acggcgcatc
    11881 aatcagcgcc gcatcaagac cttgctgctg cgttaacgcg gttttaagcg aatcgatgga
    11941 gactgcgccg acaaacttat taccgcgttc gataacgtag ccatattcgc gatcttcatc
    12001 ctgcaataat ttcagtgccg aacgtgggcc gaagccagga gttttacgaa ttaagccatt
    12061 cggtgtccgg cgggcaatat ctttcgcact gaacacctga ctaatatcaa cgccacggaa
    12121 gaaggtacgg acataatcat tcgccggatt attgagaatt tcatccggtg tgccgacctg
    12181 taccacttca ccattttgca taatggcaat tcggtcgcca atacgcatgg cttcatcaag
    12241 atcgtgggaa ataaagacaa tggtgcgctg atgtttcgcc tgtaatttta ccagctcatc
    12301 ctgcatctcg gtgcgaatta atggatcgag cgccgagaag gcttcgtcca ttaataatat
    12361 atccggatta atcgctaacg cgcgggctaa tcccacacgt tgacgcatcc cgccagagag
    12421 ttcatccggg tagctgtggg cataattttc cagcccgacc tgacgcagtg catcaagggc
    12481 tttttcccgg cgttcttcgg cattaattcc ggccaattcc ataccgaacg cagtattgtc
    12541 cagcacggtc atatgcggca ttaaggcaaa ggactggaag accatcgcaa tcttttttct
    12601 gcgcacctca cggagttcgg cgtcggatat tttggcaata tccacaccat caatcagcac
    12661 ttgcccgcgg gtgggttcaa tcaggcgatt gagaaggcgt accattgtgg atttacccga
    12721 gccggataat cccatgatga caaatatctc gccttcttca atggccagac tggcgtcttt
    12781 tacgccaagc gatagcccag ttttttccag aatttgttct tttgaaagtc cttgttcgat
    12841 atatttgaac gctcgctgtg gatgctcacc aaatatttta taaagatttt taatttttaa
    12901 tttaatggcc atgcaataga atgattcctt tatttgttta tgtcgatata ttacccaatg
    12961 ggaatagtca cttttttcta ccctaacata ctgagaatct gaggcaaccc ctgagggcaa
    13021 atgtggcatg aatattgatg cgagcaaatc gccggaattt tccgtgatat aagggctgag
    13081 agcaaatcga gaaaagttga tattttttgt catagatagc gaaaaaacgc ctgaatattg
    13141 tagtttcagg cgaataatgc aacagagtat cagtgtagat cactacagaa tatttgcgtg
    13201 ataaaaagct atttggcggg aattatttcc ctgctgcggg tagtgatatt tttgaaaata
    13261 acaccctcag aaattccagt cttcatcttc tgtttcaacc gctttcccca tcacataaga
    13321 ggagcctgaa ccggaaaaga aatcgtgatt ttcatcggca ttcggcgaaa gcgcggcgag
    13381 gattgccgga ttcacttccg ccatttctgc gggaaataac ggttcgtagc ccagattcat
    13441 caaagcctta ttggtgttgt aacagagaaa cgctttcaca tcgtcagccc acggggtttc
    13501 ggcgtacagc tcatcggtgt attgcaactc gttgtcgtag agttccagca acaaatcgaa
    13561 ggcgaaactc ttcaactctt cacgttgtcc cagagatatc ttttccatgt ttttctgata
    13621 tttatagcct atgtagtaac cgtggactgc ttcatcgcgg ataatcagac ggatcaggtc
    13681 cgcggtattg gtcagctttc cgcggctgga aaaatacatc ggcagccaga aaccggaata
    13741 gaacaaaaaa gattcaagaa acacactggc gattttcttt ttcagcggat catcaccgcg
    13801 ataatgttgc tgaataatct gagcttttcg ctgcaacggt gcgttttctt cactccaggc
    13861 gtaggcggca tcgacatctt tggtctggca cagcgtcgag aaaatcgaac tgtaagagcg
    13921 ggcatgaacc gcttccataa agctgatatt cgataatacc gcctcttcat gaggcgtgag
    13981 cgcatcgggc atcagcgaag gcgcgccgat aacattttgc agcgtgtcga gcagcgtcag
    14041 gccagtaaaa acgcgcatcg tcagttgttg ttctacgaca gttaatgtct gccaggcggg
    14101 aatatcgttc gacagcggca ccttttctgg tagccagaaa ttgctggtca ggcgattcca
    14161 cacctccaga tctttatcgt cagatatctt gttccagttg atggcgctga tacgtgagag
    14221 tttcatagat attccttaaa gtgcacagga gacgcagcct tcaatttcag tgccttccag
    14281 cgccatctga cgcaggcgga tgtaatagag cgttttgata cctttgcgcc aggcgtaaat
    14341 ctgcgctttg ttgatatcgc gagtggtggc ggtatcgggg aaaaacagcg tcagcgacag
    14401 cccctgatcg acatggcgag tcgcttccgc gtaggtgtcg atgatctttt ctgcgccaat
    14461 ttcgtaagcg tcctgataca gcgccagatt ttcgttagtc ataaacgggg cagggtagta
    14521 aacgcgtcct gttttgccct ctttgcgtat ctctactttc gccacaatcg gatgaatact
    14581 cgacgtagca tggttgatat aagagataga cccggttggc ggcaccgcct ggagattctg
    14641 gttgtatatg ccgtagcgca tcacgtcgtc gcgcagttgc gcccacatct cacgggtagg
    14701 taacgtaata cagctgcggg caaacagttc gccaactttc gccgttttcg gctgccagtt
    14761 accttgcaga tattggctga aatattcacc actggcatag cgtgactgtt tgaacccggc
    14821 gaaggtttca ccgcgttcgc gcgccagcaa catcgaggta cgcagtgcgt gccaggtgat
    14881 ggtatagaaa tagagattgg tgaaatccag ggcttccggc gaaccataag cgatgccttc
    14941 cctcgccaga taaccatgta aattcatctg ccccagtccg atggcgtgcg aggcggcatt
    15001 tccggcttcg atggacggca cgctgcggat atgactcata tctgacaccg ccgttaaacc
    15061 gcgcacggca gtctctaccg tgcgggaaaa atcgggggaa tccatggtgt gcgcaatatt
    15121 caacgaacct aaattgcagg aaatatcatg gcctgtgcgg gcatagtcga gattctcgtc
    15181 atactctgag gcgctgttaa cctgcaaaat ttctgagcag agattactca tatttatgcg
    15241 cccggcgata gggctagcac ggtttactgt gtcttcatac atgatgtagg gatagccgga
    15301 ctcaaactgg atttctgcca gtcgctggaa gaaatcacgg gcgttgaggt attttttgcg
    15361 gatgcgttca tcggcaacca gttcgtcata gtgttcgcta atggcgatat cggcaaacgg
    15421 cttgccataa actcgctcaa cgtcataggg cgaaaacagc gccatctgcg cattctcttt
    15481 tgccagatgg aaagtgatat ccgggatcac cacgcccagc gacagtgttt taatgcggat
    15541 tttttcgtcg gcattttccc gtttcgtgtc gagaaaacgc agaatatcgg gatgatgagc
    15601 atgtaaatag actgcacctg ccccctgacg cgcgccgagt tggttggcat aggaaaatgc
    15661 gtcttccaac attttcatca ccggaattac gccagaagat tgattttcaa tacgtttaat
    15721 tggcgcgccc gcttcccgca gattcgacag caaaaatgct acgccgccgc cgcgtttcga
    15781 cagctgcagt gcggaattta ccgcccgacc aatcgactcc atattgtctt caatacgcag
    15841 caaaaaacag gaaaccagtt cgccgcgctg ctgcttaccg cagttgagga atgttggcgt
    15901 ggctggctga aagcgtcctg acagcatttc atcggtcagt tgcaacgcca gcgtctcatc
    15961 gccttgtgcc agcgtcagcg ccaccatcgt gactcgatcg gcaaaatctt ccagataacg
    16021 tttaccgtcg aatgtcttca acgtatagct ggtgtaaaac ttccatgccc cgaggaatgt
    16081 ctggaaacga aaaccgctgg tgtgtgcgtg ggcaaacagc gtaatgacaa agtcgcgaga
    16141 gtagcgatta agaacgcttt catcatagta accttcgttg accagccagt tcaggcgctg
    16201 ctgctggcta ctgaaggtca cactgttcgg acgcacatgc gtcgcaataa aggcgtcaac
    16261 ggcctggcgg tctttatcga actgaatgcg acctgcgcta tcgtaaagat taagcatcgc
    16321 attcagcgcg tggtaatcca tcgtttcctg cgtcaggcat tctgcggttg tcgttgccaa
    16381 aattcggtta ctcctttacg aacgttttcg atatcgcttt gcgtacccat gagttcaaaa
    16441 cggtacagcc acggcacgcc gcatttccgg gcaatcacat ctccggcgcg gccatacgcc
    16501 tcaccaaagt tgcgattacc agaagcaata acgccgcgaa gcaacgcccg gttgtgctcg
    16561 tcgtttaaaa agcgaattac ctgtcgtggc accgcgccag ccgtaccgcc gccgccgtaa
    16621 gagggcacga tcaggatgta aggctcgtct acctgaatcc gttcccgctc attgagcggg
    16681 atgcgcaccg cgggcagacc taaacgttcg ataaaacgct gcgtgttttc ggagctgctg
    16741 gagaagtaga cgagctggct catgcactgg ccgcgtgtgg cgctggatgc agacggttaa
    16801 tcatgtccgg acggaaacca gaccagctaa gatcgccagc aatcactacc ggcaactgac
    16861 gaaagccctg agcacgcaac gcttccgctg cttcaggaac gcgatcgaca ttaatcattt
    16921 caaaatcaaa gccccggttt tccatcgccc gtttggtggc gtggcactga acgcaatcgt
    16981 tacgagtata aatagttatg cgcatgattc gtatttccgt ttaaaatgaa gatacggcgc
    17041 gatgatacgc gtcgggttgt ctctctgttg atacagagat actagatgta gttgaaaaaa
    17101 gattcaacca cacaatatat agcaaatagt gatcgaaatt accctggata tgagcgtgac
    17161 gggggagggg gatttttgtg attcaccagg caaaaagaaa ccccgaagac aggcttcggg
    17221 gtcaaagacg cgtatttatt atcatttttg cactacgatt tgcgcacgct taacagtgcg
    17281 ccgataaaaa tacctaccgc agctgctgta cccacgctac accagggtct ttcacgaaca
    17341 aaagaatccg ctcagccaac ggcatcgcgc gcggcttgct ggacgcgagt acgaccatgc
    17401 attcgcgccc tggtttcttt cagcaacgcc tgagctttac tgcgtgcggc ttcagcttcc
    17461 cctttggcgt cgctgcccca ggacttcaat acagattcga ggctgtcagc taattgattg
    17521 acatcattct ggatatcctg cacgccatca tcaacgtcgt tgcggttcgg tcggttatac
    17581 atatgatccc ccattagaat tcgatgtaat tttcagtgta gaccataatt ttcttaactg
    17641 aggttcatcg cggctttatg gacttttctg aaagcgttgt gaatgcgcaa tcaggtaagg
    17701 taggcccgcg gttgaaaata acgaggatca aatatgtatt tacgaccaga cgaggtggcg
    17761 cgcgtacttg aaaaagtcgg ttttactgtc gatgtggtaa cccagaaagc gtatggttac
    17821 cgccgtgggg aaaattatgt ctatgttaat cgcgaagcgc gaatgggacg taccgctctg
    17881 gtaattcatc caacattaaa agaacgcagt tcaacactgg ctgaacccgc ttccgatatt
    17941 aaaacttgcg atcattacca gcaatttccg ctctatttag caggcgagcg acacgagcat
    18001 tacggtatcc cgcatggctt tagttcgcgt gttgcgcttg aacgttattt gaatggttta
    18061 tttggcgaag ccagttaaag acgcgactgg cgatgccagt cgcgaaaaga agagtcaggc
    18121 ttttacctgc tggtaacggc tgactttaaa cagtcggcgg caataatcga ggaagtagcc
    18181 ataaaccgcc cccatcaaca tcgaaacaac gatgtttgaa ctgaccgccg ccataatctg
    18241 atgccagtct gcgcccacca ctaacaagat cgccacatac accggtgact ggaacgtcac
    18301 ataagccagg atatcagcca gattttttat ccagcccgac gggctaactt tgcgtgccgc
    18361 gcgcataaac agatcgcggt acataccgta tggccatgca attaagatgt tcaccggaat
    18421 cgctaccaat ctggaataaa aagactgttc gaagctcatt ccggagagga aaacttcaat
    18481 acacatgttc acgacagaac agtaaacaac catcgcgaac gtatctgcaa ctgcatgacg
    18541 caagcgtgac tgcggtgaga acatgctaat gctccttaat aaaagcgtaa tgggatgtta
    18601 atggagatga aagcagtgga tctcattgcc atctgcattt ttgcatagcg tatatcgctg
    18661 tctatgacta atcaattagt gaaaaatttt tatttagtta gtttttgtag ataaaatatt
    18721 ccgtaacggc tcgttttttg tacattgtgg atttttacat gtcgatatga tttattttca
    18781 attaaatcaa ttagatatga tcttgccttt aatttcacta atgaaagttt gggggggagg
    18841 taacgctata agcgtagaga attcggtgtt tgtcgtgacc gggtcattta cagcattttt
    18901 gatgccagaa aatgtacatt aacagcagaa agcaccagtg aggggtgttt tcttttattc
    18961 tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggagtta gcgagcagag
    19021 agcgccactc tgttcaccaa taacctaaat tactcatcaa aatggctaat atataatctt
    19081 gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca ggactgtaaa
    19141 tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac gaaatacttt
    19201 tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa gtttaaataa
    19261 cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg aagaaatgct
    19321 cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc agcagcgtga
    19381 actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaaag ctgacggaat
    19441 taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgtgctg gtaaaaaacg
    19501 ccagccgcgt ccggcgaaat ataaattcac tgatgttaac ggtgaaacta aaacctggac
    19561 cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta aatctctcga
    19621 cgatttcctg atctaattac tgaggccgga tgtcgctgct agggcgcgtc cggcgtcgct
    19681 tctcaagctc gttggttcca cggcatctta tctaacaacc ttgccattcc acaaaaaccg
    19741 ctgattcctg caaacagtaa cccggcacca acaaagccac ttaataagaa gaaaccgcta
    19801 tttacggtat aacccagtac aacgccgatt aatattaaac cacccgcagc gatctgcacc
    19861 tggcgcatta acggcaaggg ttgagattta tttaccgcta ctggcaatcc agctcttttc
    19921 cagccatcaa tcccatcttc gagtaaaaat atttctgcgg gggcggcaat cgctgctaat
    19981 ttatcggcgt tattactggt gcgtttacct gcctggcagt ggaatataat ttgctcgcga
    20041 cgtaatttag gtggaagacc tgactgttcc agcacggata atggagccag atctgcttca
    20101 ggaatatgtt cacgaagata ttcatcagcg tcacgaatat cgattaactt tgcgccgcgt
    20161 gcgattaatt cttgtgcatc atgcggcgaa atggttgtca aagccatagt gactccttac
    20221 ggacaataga catttttcag ggtggcgata atggcattta ccgcctcatt tttaatggaa
    20281 tatagaatgc gttgggcatc ccgttggctg ttgataagcc cttcgtcccg catacgagcg
    20341 agatgctgtg atgtcgcaga ggcgctcagt ccggtaatgc gcgtcagctc tcccgcgctg
    20401 gtgccggggg aaccgctaag catgcacaga atcagcaacc gtttagggtg gctcattgct
    20461 ttcaataagg ccgctgcctg ttcggtactg gcctgtaatt gcgcgagttc agtcatattt
    20521 atttaagtat tttctaaatt aagtaaactc taaactaaaa atgcaacata taccagcctc
    20581 agcagcgtaa atgagagtaa aagcgtaagc tgaatagact ggccccctga atctccagac
    20641 aaccagtat
//
LOCUS       DADLDT020000073        20584 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000073 DADLDT020000000
VERSION     DADLDT020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20584
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.073"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..219
                     /locus_tag="JQH22_002558"
     CDS             <1..219
                     /locus_tag="JQH22_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2206834.1"
                     /translation="SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIE
                     YYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            321..473
                     /gene="hokA"
                     /locus_tag="JQH22_002559"
     CDS             321..473
                     /gene="hokA"
                     /locus_tag="JQH22_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFB2206835.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(660..872)
                     /gene="cspA"
                     /locus_tag="JQH22_002560"
     CDS             complement(660..872)
                     /gene="cspA"
                     /locus_tag="JQH22_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFB2206836.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1153..1443)
                     /locus_tag="JQH22_002561"
                     /pseudo
     CDS             complement(1153..1443)
                     /locus_tag="JQH22_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1877..2587
                     /locus_tag="JQH22_002562"
     CDS             1877..2587
                     /locus_tag="JQH22_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFB2206837.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="JQH22_002563"
     CDS             complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="JQH22_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFB2206838.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3715..4365)
                     /locus_tag="JQH22_002564"
     CDS             complement(3715..4365)
                     /locus_tag="JQH22_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFB2206839.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4528..6861
                     /gene="bisC"
                     /locus_tag="JQH22_002565"
     CDS             4528..6861
                     /gene="bisC"
                     /locus_tag="JQH22_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFB2206840.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6830..7270)
                     /locus_tag="JQH22_002566"
     CDS             complement(6830..7270)
                     /locus_tag="JQH22_002566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFB2206841.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7267..7830)
                     /gene="tag"
                     /locus_tag="JQH22_002567"
     CDS             complement(7267..7830)
                     /gene="tag"
                     /locus_tag="JQH22_002567"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFB2206842.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7988..8686
                     /locus_tag="JQH22_002568"
     CDS             7988..8686
                     /locus_tag="JQH22_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFB2206843.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYVALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8915..10123
                     /locus_tag="JQH22_002569"
     CDS             8915..10123
                     /locus_tag="JQH22_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206844.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10447..12138
                     /gene="eptB"
                     /locus_tag="JQH22_002570"
     CDS             10447..12138
                     /gene="eptB"
                     /locus_tag="JQH22_002570"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFB2206845.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12230..12306
                     /locus_tag="JQH22_002571"
     tRNA            12230..12306
                     /locus_tag="JQH22_002571"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Pro,seq:cgg)
     gene            13050..14657
                     /gene="dppA"
                     /locus_tag="JQH22_002572"
     CDS             13050..14657
                     /gene="dppA"
                     /locus_tag="JQH22_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFB2206846.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14965..15984
                     /gene="dppB"
                     /locus_tag="JQH22_002573"
     CDS             14965..15984
                     /gene="dppB"
                     /locus_tag="JQH22_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFB2206847.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15994..16896
                     /gene="dppC"
                     /locus_tag="JQH22_002574"
     CDS             15994..16896
                     /gene="dppC"
                     /locus_tag="JQH22_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFB2206848.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16907..17890
                     /gene="dppD"
                     /locus_tag="JQH22_002575"
     CDS             16907..17890
                     /gene="dppD"
                     /locus_tag="JQH22_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFB2206849.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17887..18891
                     /gene="dppF"
                     /locus_tag="JQH22_002576"
     CDS             17887..18891
                     /gene="dppF"
                     /locus_tag="JQH22_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HFB2206850.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18921..20192)
                     /locus_tag="JQH22_002577"
     CDS             complement(18921..20192)
                     /locus_tag="JQH22_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFB2206851.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4947 a         5071 c         5403 g         5163 t
ORIGIN      
        1 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
       61 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      121 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      181 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      241 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      301 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      361 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      421 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      481 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      541 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      601 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
      661 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      721 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      781 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      841 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      901 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      961 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1021 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1081 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1141 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1201 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1261 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1321 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1381 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1441 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1501 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1561 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1621 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     1681 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1741 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1801 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1861 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1921 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1981 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2041 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2101 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2161 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2221 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2281 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2341 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2401 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2461 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2521 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2581 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     2641 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2701 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2761 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2821 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     2881 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2941 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3001 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3061 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3121 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3181 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3241 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3301 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3361 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3421 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3481 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3541 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3601 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3661 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3721 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3781 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3841 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     3901 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3961 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4021 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4081 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4141 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4201 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4261 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4321 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4381 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4441 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4501 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4561 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4621 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4681 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4741 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4801 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4861 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4921 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     4981 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5041 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5101 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5161 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5221 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5281 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5341 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5401 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5461 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5521 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5581 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5641 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     5701 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5761 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     5821 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     5881 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     5941 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6001 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6061 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6121 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6181 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6241 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6301 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6361 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6421 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6481 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6541 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6601 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6661 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6721 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6781 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6841 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6901 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6961 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7021 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7081 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7141 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7201 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7261 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7321 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7381 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7441 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7501 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7561 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7621 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7681 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     7741 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7801 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7861 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7921 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7981 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8041 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8101 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8161 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8221 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8281 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8341 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8401 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8461 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8521 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8581 gttgctcaat caaaatattg ccgcctatgt tgcgctaact caggcagaaa ataccactaa
     8641 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8701 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     8761 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8821 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     8881 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8941 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9001 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9061 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9121 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9181 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9241 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9301 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9361 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9421 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9481 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9541 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9601 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9661 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9721 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9781 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9841 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9901 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9961 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10021 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10081 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10141 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10201 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10261 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10321 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10381 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10441 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10501 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10561 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10621 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    10681 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10741 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10801 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10861 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10921 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10981 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11041 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11101 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11161 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11221 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11281 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11341 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11401 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11461 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11521 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11581 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11641 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11701 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11761 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11821 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11881 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11941 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12001 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12061 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12121 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12181 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12241 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12301 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12361 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12421 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12481 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12541 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12601 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    12661 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    12721 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    12781 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    12841 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    12901 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    12961 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13021 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13081 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13141 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13201 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13261 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13321 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13381 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13441 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13501 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13561 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13621 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    13681 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    13741 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    13801 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    13861 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    13921 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    13981 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14041 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14101 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14161 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14221 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14281 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14341 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14401 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14461 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14521 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14581 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    14641 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    14701 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    14761 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    14821 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    14881 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    14941 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15001 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15061 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15121 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15181 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15241 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15301 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15361 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15421 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15481 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15541 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15601 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    15661 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    15721 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    15781 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    15841 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    15901 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    15961 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16021 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16081 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16141 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16201 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16261 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16321 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16381 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16441 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16501 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16561 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16621 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    16681 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    16741 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    16801 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    16861 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    16921 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    16981 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17041 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17101 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17161 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17221 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17281 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17341 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17401 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17461 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17521 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17581 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    17641 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    17701 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    17761 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    17821 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    17881 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    17941 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18001 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18061 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18121 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18181 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18241 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18301 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18361 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18421 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18481 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18541 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18601 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    18661 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    18721 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    18781 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    18841 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    18901 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    18961 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19021 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19081 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19141 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19201 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19261 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19321 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19381 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19441 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19501 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19561 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19621 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    19681 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    19741 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    19801 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    19861 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    19921 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    19981 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20041 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20101 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20161 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20221 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20281 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20341 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20401 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20461 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20521 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    20581 tacc
//
LOCUS       DADLDT020000074        20570 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000074 DADLDT020000000
VERSION     DADLDT020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.074"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            409..1239
                     /locus_tag="JQH22_002578"
     CDS             409..1239
                     /locus_tag="JQH22_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFB2206852.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQH22_002579"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQH22_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFB2206853.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="JQH22_002580"
     CDS             complement(2368..3828)
                     /locus_tag="JQH22_002580"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFB2206854.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQH22_002581"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQH22_002581"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFB2206855.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="JQH22_002582"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="JQH22_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFB2206856.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQH22_002583"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQH22_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFB2206857.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQH22_002584"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQH22_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFB2206858.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQH22_002585"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQH22_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFB2206859.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="JQH22_002586"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="JQH22_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFB2206860.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="JQH22_002587"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="JQH22_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="JQH22_002588"
     CDS             complement(12470..13009)
                     /locus_tag="JQH22_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFB2206861.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="JQH22_002589"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="JQH22_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFB2206862.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="JQH22_002590"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="JQH22_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFB2206863.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="JQH22_002591"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="JQH22_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="JQH22_002592"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="JQH22_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFB2206864.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="JQH22_002593"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="JQH22_002593"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFB2206865.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="JQH22_002594"
     CDS             19140..20120
                     /locus_tag="JQH22_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFB2206866.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4672 g         5512 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta aatttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgattcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgacatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DADLDT020000075        20523 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000075 DADLDT020000000
VERSION     DADLDT020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.075"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="JQH22_002595"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="JQH22_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFB2206867.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="JQH22_002596"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="JQH22_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFB2206868.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="JQH22_002597"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="JQH22_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFB2206869.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="JQH22_002598"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="JQH22_002598"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFB2206870.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="JQH22_002599"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="JQH22_002599"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFB2206871.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="JQH22_002600"
     CDS             4451..4816
                     /locus_tag="JQH22_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFB2206872.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="JQH22_002601"
     CDS             4966..5484
                     /locus_tag="JQH22_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFB2206873.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="JQH22_002602"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="JQH22_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="JQH22_002603"
     CDS             6715..7197
                     /locus_tag="JQH22_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFB2206874.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="JQH22_002604"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="JQH22_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFB2206875.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="JQH22_002605"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="JQH22_002605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFB2206876.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="JQH22_002606"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="JQH22_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFB2206877.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="JQH22_002607"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="JQH22_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFB2206878.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="JQH22_002608"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="JQH22_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFB2206879.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="JQH22_002609"
     CDS             10941..11732
                     /locus_tag="JQH22_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFB2206880.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="JQH22_002610"
     CDS             11753..13039
                     /locus_tag="JQH22_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFB2206881.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="JQH22_002611"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="JQH22_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFB2206882.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="JQH22_002612"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="JQH22_002612"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFB2206883.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="JQH22_002613"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="JQH22_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFB2206884.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="JQH22_002614"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="JQH22_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFB2206885.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="JQH22_002615"
     CDS             complement(16987..17277)
                     /locus_tag="JQH22_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFB2206886.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="JQH22_002616"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="JQH22_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFB2206887.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="JQH22_002617"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="JQH22_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFB2206888.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="JQH22_002618"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="JQH22_002618"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="JQH22_002619"
     CDS             19139..20380
                     /locus_tag="JQH22_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFB2206889.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5303 a         5360 c         4814 g         5046 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctag aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacatg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacagat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacagacaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacattccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttacc tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aggaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgagc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DADLDT020000076        20319 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000076 DADLDT020000000
VERSION     DADLDT020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.076"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="JQH22_002620"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="JQH22_002620"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFB2206890.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="JQH22_002621"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="JQH22_002621"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFB2206891.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="JQH22_002622"
     CDS             complement(3122..3421)
                     /locus_tag="JQH22_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFB2206892.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="JQH22_002623"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="JQH22_002623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFB2206893.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="JQH22_002624"
     CDS             complement(4633..5070)
                     /locus_tag="JQH22_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HFB2206894.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="JQH22_002625"
     CDS             complement(5116..5457)
                     /locus_tag="JQH22_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206895.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="JQH22_002626"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="JQH22_002626"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFB2206896.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="JQH22_002627"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="JQH22_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="JQH22_002628"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="JQH22_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2206897.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="JQH22_002629"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="JQH22_002629"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFB2206898.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="JQH22_002630"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="JQH22_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFB2206899.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="JQH22_002631"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="JQH22_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="JQH22_002632"
     CDS             complement(13973..14797)
                     /locus_tag="JQH22_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFB2206900.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="JQH22_002633"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="JQH22_002633"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFB2206901.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="JQH22_002634"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="JQH22_002634"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFB2206902.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="JQH22_002635"
     CDS             complement(18729..19202)
                     /locus_tag="JQH22_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFB2206903.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="JQH22_002636"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="JQH22_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFB2206904.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
BASE COUNT         5108 a         5168 c         4972 g         5071 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact gtttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcc
//
LOCUS       DADLDT020000077        20288 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000077 DADLDT020000000
VERSION     DADLDT020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.077"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="JQH22_002637"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="JQH22_002637"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFB2206905.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="JQH22_002638"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="JQH22_002638"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFB2206906.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="JQH22_002639"
     CDS             complement(1727..2998)
                     /locus_tag="JQH22_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206907.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="JQH22_002640"
     CDS             complement(3181..4386)
                     /locus_tag="JQH22_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2206908.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="JQH22_002641"
     CDS             complement(4486..4842)
                     /locus_tag="JQH22_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFB2206909.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="JQH22_002642"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JQH22_002642"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JQH22_002643"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JQH22_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFB2206910.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="JQH22_002644"
     CDS             6772..9828
                     /locus_tag="JQH22_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFB2206911.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="JQH22_002645"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="JQH22_002645"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFB2206912.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="JQH22_002646"
     CDS             10335..10523
                     /locus_tag="JQH22_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFB2206913.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="JQH22_002647"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="JQH22_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFB2206914.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="JQH22_002648"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="JQH22_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFB2206915.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="JQH22_002649"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="JQH22_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFB2206916.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="JQH22_002650"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="JQH22_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFB2206917.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="JQH22_002651"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="JQH22_002651"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFB2206918.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="JQH22_002652"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="JQH22_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFB2206919.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="JQH22_002653"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="JQH22_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFB2206920.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="JQH22_002654"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="JQH22_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFB2206921.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="JQH22_002655"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="JQH22_002655"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFB2206922.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5315 a         4856 c         5176 g         4941 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgata
//
LOCUS       DADLDT020000078        20146 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000078 DADLDT020000000
VERSION     DADLDT020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20146
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.078"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..707)
                     /locus_tag="JQH22_002656"
     CDS             complement(<1..707)
                     /locus_tag="JQH22_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2206923.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNV"
     gene            complement(707..1387)
                     /locus_tag="JQH22_002657"
     CDS             complement(707..1387)
                     /locus_tag="JQH22_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFB2206924.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1384..2583)
                     /locus_tag="JQH22_002658"
     CDS             complement(1384..2583)
                     /locus_tag="JQH22_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFB2206925.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2584..2937)
                     /locus_tag="JQH22_002659"
     CDS             complement(2584..2937)
                     /locus_tag="JQH22_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206926.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(2937..3677)
                     /locus_tag="JQH22_002660"
     CDS             complement(2937..3677)
                     /locus_tag="JQH22_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFB2206927.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            3900..4190
                     /locus_tag="JQH22_002661"
     CDS             3900..4190
                     /locus_tag="JQH22_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFB2206928.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4214..4558)
                     /locus_tag="JQH22_002662"
     CDS             complement(4214..4558)
                     /locus_tag="JQH22_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206929.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4571..5635)
                     /locus_tag="JQH22_002663"
     CDS             complement(4571..5635)
                     /locus_tag="JQH22_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206930.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5638..5943)
                     /locus_tag="JQH22_002664"
     CDS             complement(5638..5943)
                     /locus_tag="JQH22_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206931.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(5945..6547)
                     /locus_tag="JQH22_002665"
     CDS             complement(5945..6547)
                     /locus_tag="JQH22_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206932.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6547..8562)
                     /locus_tag="JQH22_002666"
     CDS             complement(6547..8562)
                     /locus_tag="JQH22_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFB2206933.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8740..9165)
                     /locus_tag="JQH22_002667"
     CDS             complement(8740..9165)
                     /locus_tag="JQH22_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206934.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9169..9609)
                     /locus_tag="JQH22_002668"
     CDS             complement(9169..9609)
                     /locus_tag="JQH22_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HFB2206935.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9620..10765)
                     /locus_tag="JQH22_002669"
     CDS             complement(9620..10765)
                     /locus_tag="JQH22_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HFB2206936.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10769..11326)
                     /locus_tag="JQH22_002670"
     CDS             complement(10769..11326)
                     /locus_tag="JQH22_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206937.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(11301..11690)
                     /locus_tag="JQH22_002671"
     CDS             complement(11301..11690)
                     /locus_tag="JQH22_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HFB2206938.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11677..12231)
                     /locus_tag="JQH22_002672"
     CDS             complement(11677..12231)
                     /locus_tag="JQH22_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206939.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12228..12635)
                     /locus_tag="JQH22_002673"
     CDS             complement(12228..12635)
                     /locus_tag="JQH22_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HFB2206940.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            12634..12868
                     /locus_tag="JQH22_002674"
                     /pseudo
     CDS             12634..12868
                     /locus_tag="JQH22_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12865..13806)
                     /locus_tag="JQH22_002675"
     CDS             complement(12865..13806)
                     /locus_tag="JQH22_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFB2206941.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(13818..14321)
                     /locus_tag="JQH22_002676"
     CDS             complement(13818..14321)
                     /locus_tag="JQH22_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206942.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(14326..15546)
                     /locus_tag="JQH22_002677"
     CDS             complement(14326..15546)
                     /locus_tag="JQH22_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFB2206943.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(15561..16298)
                     /locus_tag="JQH22_002678"
     CDS             complement(15561..16298)
                     /locus_tag="JQH22_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFB2206944.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(16186..17652)
                     /locus_tag="JQH22_002679"
     CDS             complement(16186..17652)
                     /locus_tag="JQH22_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFB2206945.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(17652..19274)
                     /locus_tag="JQH22_002680"
     CDS             complement(17652..19274)
                     /locus_tag="JQH22_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HFB2206946.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(19277..19849)
                     /locus_tag="JQH22_002681"
     CDS             complement(19277..19849)
                     /locus_tag="JQH22_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFB2206947.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(19833..>20146)
                     /locus_tag="JQH22_002682"
     CDS             complement(19833..>20146)
                     /locus_tag="JQH22_002682"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFB2206948.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         4491 a         5640 c         5080 g         4935 t
ORIGIN      
        1 tggacatttg tcgccgatac agtcggctgc cctgacgcgc cagtaacgtt caatgtaact
       61 gtgacagcaa ggactcttga gaatgtcatc gggaacctca tcccgtatgt tgaagataca
      121 ttcggggagc cattcaactg accgcactgt aaaattaatc cagacggaaa tttttgcgca
      181 gcagccgttg cggtattact tgacgcgaac aggctcatat caggtatctg attcgcccct
      241 gtacctacat tccttttagc cgcttctccc aaaccaaggt atgtgagaat gtcagcaata
      301 gtatttttcc caataatgtc acggccaaca gaagttaaat cagtctgcgc tgctgtatca
      361 tttccagtga aatatgggag tttatttgca cctgttgcga gcccagctaa tgccgacagc
      421 gtggcatcaa gcgcctggaa atctttcccg aaagcggtcc ccattttgga tataaacccg
      481 ttcaggtctc catcatcaag cacatccagc ctgcttttgt tggcggtgta ctgcgccaac
      541 gctgccgcga taaagctggc ttgccgaata gctttgttga cctgtgcgct ggatgcttta
      601 cctgccgtaa accctgaaag cagagccgga agtgcttccc agtcagcttg tgaggtgacg
      661 ttagcgttcg gatcaagcgc gaaaggttta aagttgttta ttgccattag agtattgtcc
      721 cccatgctcc aacatcgaac ccgccgatgt attcgttatc catatcaaac ccaaagaatt
      781 tagagccctc ggatggtgct tctaccgaag gcgtttcaac atcaccggcc catacgccag
      841 ctgctttaac ggtgagatag ccctgtttga tagcggcaat cagttcaaga gacacatcag
      901 aaatatcagt ctcggggaat acccaaaccg atatcgtcat gtcctggttg tcgacaattt
      961 gcatcctcag gcctgagcct tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc
     1021 gaccgtccca gttgttgata gcgattttcg ctttcagaat gatgcggtac gtctcatcgc
     1081 ttagcgtcgt atagccagaa tcaggatcat atggcccttt ccagatgccc tggtcatacc
     1141 caagcccgtc agtgtcccag ctgaaataaa ctccgctaat tggctggctg actatgcgac
     1201 tgcgtccgat ccacagaccg aggatgtcga gctgtacacc gacagcagta tcaatatcga
     1261 aggctgttat aagccctgac atagtgctgg acacatcaat cagcgggcgg gtgctcagat
     1321 ctatatggtc aaaaaagagt ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt
     1381 tgctcatgag gtcaccgtga tactgatatt cgcggtgcta caggacgcag aagcatcata
     1441 ggcaatatca atgtttgatg ccgatacgct gccagacgac ttaccgatca gcaggtcggt
     1501 aatatcgtaa tagcgggcat tcccgccgct cacaacgccg aggtttgccg gggaataaat
     1561 acggctcagc agaacgtcgt cgccaattgt taggccattt atataatcgg caacagcctg
     1621 tttaatctgc tcgccgattt gagaggtata gccggtaaaa actttcaggg taatggctac
     1681 gaaaattggc acatcggtag agcgcgaaaa actgatgacg tgtggattac cgtaagtatc
     1741 cggcaccgtg acagaagttt taccgtaagt tgcggttccc tgccctttac tcccccggat
     1801 tgtttgggct atctcggtaa catcccctcc atcgacgatg gcggaaatag agtgtggcgg
     1861 cagcccgttg ctgtcggttg ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc
     1921 agcaacatta gcgatagcac cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac
     1981 gctttgcccc tgccgaatgc gcagctctgc gtcggtttcg gctggtgaac cgacagtagc
     2041 cgcaactgga ttggttaccg acacccaacc tcgggtcggg gtgttgatag tggtaatagt
     2101 cccggccagc gccgcaaccg aaccgctatt cgcacatgtg gccgtcacca gcacagtacc
     2161 atcaacgccg atcgctacac ttgcgggaaa attccagata atgccgtttt tatcccgtgc
     2221 ggagccattc gtgatagtcg tgcctgccgt accggttaac agaaggtcag cagtagagtt
     2281 tgtcgctact tttcgcgtga tcccgttaat tttcacattg ctgctaagcg ctgcggcctg
     2341 cgctgtcgtc ggtgaaaaag agttgtagat cccgatagcg gtgttgttag cgtcatgcac
     2401 ggcaagagcc accagcgcga ccatctgccc gtctttgctg tctggttcga ggtaggcatc
     2461 actaccgtaa atctgcctga aatagctggt cagtgtatct aggattgtct ggtaatcagg
     2521 cgcactaatc ccctgggcgg ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc
     2581 catttatgcc tcgcttgtta cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa
     2641 ggtgacgcga cggctggtgc cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc
     2701 cggtgtatcc tgtatgcgtt cgcgtatagc caggatgtag acatctgatc gctgcttccc
     2761 aagcactgac tgaacatacg gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca
     2821 cagctcgaaa cgggttttta cggcctgggc gacacattcc ggactgtcga taaggaaagt
     2881 atcgtcaccc tggccgaaag tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat
     2941 tgcggccctc cggtagttcc cccgccagtc tgtactccgc catgtttatg cgtggcgaca
     3001 ctgataccag aagctgttac gtcattcgtt accatcaccg gcccaagcat cgtcgcagta
     3061 ccgccgcttt cgcccattcc ctgagacaag ttgccattaa tcgttacgtt gccgttcagc
     3121 gtgatagtcg gggatgtgat tgtcgttcca ccttcagccg tagctgtaag ctggcccggt
     3181 gttttaatgg tgatgttatg tcctgcggcg acttctacaa acgccgcacc atcatcggtt
     3241 cgcagctgcg cggcgctggt actgataccg ctgattttcc gtgcttgcga ctgcgggcca
     3301 acgatggcga acgcatcaga taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg
     3361 ccgctctgcc accaaaaatc gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct
     3421 tcttttaccg gaaaggtcag cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc
     3481 accagcggtt ttaattcggt ggatccatcg ccaacaatac cgcgtagcgc cacctctact
     3541 gtgcaggtaa cagtctcagg atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc
     3601 tgggtagaca ccgaatcggc aatggcctgc gcggtctgct gctcaccgcc gatctgtgat
     3661 tgagttggaa ttggcataaa acccccataa aaaaaccagc cgaagctggt ttggtatcaa
     3721 ttatctctta gtaactaagc tacatcagtg tgaagtaagg cttttccgct gcaatggcac
     3781 acattcacaa ggtgtattag gatatgtttg caacaagtaa gcattgaaaa tcaatttcag
     3841 ccatgaagcc gccatagagt ggacctgcac catcgcatta ttgactacag gagtaacaaa
     3901 tgggatttag atttcgtaaa agaatccgga ttgcgcccgg actcgcgatc aacattagta
     3961 aaagcggagt aagcacttca attggtggta aaggttccac cattaacatc gggaaaaaag
     4021 gcgtaaagat gacaaatggc cttcccggca cggggctgtc gcataccact aacctttact
     4081 ctcccgggaa atcgacagaa aaaaaacaac ttactcataa gcaaaaaata attagaaaca
     4141 tcctgttcgt aattattgtg tttataataa ttaaagctca atatttttga cgcatgcccg
     4201 cctatctggc gggctatttt acttttcggc agtcgtatgt tgcatactga cgcggcgaat
     4261 ccatgctggc ttgcagccac tgtgcgttga gaatggcttt gccgtttcga ttgatgtact
     4321 caagaccaac ccatcttcca ggctgatcgg tagccatacg ccaatccatc ttgatattgt
     4381 tataatcgcc tttgtttttc aggaaggtga ttttttgcat ttctggcttt gcgccattga
     4441 ttctggctaa gccatcatct gcccaatgga ttttaaaatc accacattgc tgatccgcaa
     4501 aagcgggtgc tgatataagc accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg
     4561 ccccacctca ctaattcgtc gtcccttgca ttgctttcgg gctataaaga tcacgagcgc
     4621 cacgcgcaaa acacatcaaa tccatgtacc acgcctgacc tctggtgtcg ccagtatagt
     4681 cgatagcttt gacgatataa acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc
     4741 cggtcagcac acggttgccg ttctcttctg tttcggtgat acgcccgggc gactgtgcta
     4801 tttcgctatt gccgagcgtg gcgcggtaca ccgaagcctg atcgagctgg ataagaccat
     4861 taatacggat gtttgggttt atcaggcatc gcacgtttac tccgccaccc atcgtttgtt
     4921 gcggcatacc gatcaggcca gtattggcat tcaacacaat ggcttcgtga atatatttat
     4981 cctccggcac catctggacc tgaccatcca ccagttgcca tgtcgctttg cactgcgcag
     5041 caatattatc catcacgttg cggctggatg agtaaatcgc gcggccacga ggaaacacgg
     5101 tatcaggaaa atcgccggta atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg
     5161 tcgcccggtg cagatccgca acggtatatc cagcggcaag cgtggtgatg gtggtcgcat
     5221 agaggaacgc ttcgtgatca ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt
     5281 ccttcccggt gacggtgaag cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt
     5341 tcacctgccc tatctggtct ggtgaaatct cccgggacac accaacctgg ctcgcatcaa
     5401 catccggcgc tataccgtca tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa
     5461 gtatcttgtt cgtggtatcg gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc
     5521 agcgtgtgtc tgcccactcg atctggaacg tgaccttaaa gtcagacagg gaaacgccct
     5581 gcccgttctg gtccaacagt tgcagctcaa aatggcgcat ccagttaaga gacatttcta
     5641 ctcctgtacg aaaatgaggt ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg
     5701 tgcgccctta tcggtcgcca ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg
     5761 taaaaggttt acgccggtca gtagaggtac gccagagaga agcgcggcac cgccgctatc
     5821 catcacgtcc ataatccagc cagccgcatc acgccagatg atcctgagtg tatacgtggt
     5881 attgcccagc aaaactcgga actgctgatt gtcaggagaa agcggtattt cgttaaactg
     5941 catatcatcc cccgaatgct gatgtaacgc ttccgcccag ctggctcagc aaggattcgt
     6001 ttggcggtgt agtggatttc atcccggaat tctgcaccgc cgatgtgctg acgccatcct
     6061 gcatatctga tttatctgca acgctaacgc tctgcgtctg cgacatgatc acttcacgca
     6121 gggtaagcgt gcagttcagc acgttctcgc tggttttatc cgttgtcacc tcgatggctc
     6181 gcaccagcat attgctgtaa acccgctttc cggtcactac atcgaacggc accctagagg
     6241 actggagatc cagtagttgc tgataggtct ctttcgggct aagtccggcg ctgaggccga
     6301 ttgacgatgt atcaatgaag tccagcaacg aaccgccacc tgcgaagccg cattccatcg
     6361 tgacttcgct ggggcgctta tacgcatgat cggcgatgaa acccgacgcg ctattcgttg
     6421 ttggcttctc caccgggtgc tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa
     6481 ccacgctggg gattaatatt ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa
     6541 tatccattat ctcggtcctg cggggagttg ctgggttaac tgtgaattca cgcccttttg
     6601 acggtcaaca gtcaaacggg cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt
     6661 cgtttcctgc tgaatcaccg gggcgctggt gggcatattg ctcattactt tcggaatgta
     6721 gttgcgcgtt tcctgcggca ttagccccat tccataacgc ttaacattcc cgatccccca
     6781 gttatatgat gccagtgctt tgctaaggtc tccgccgttc tgccgcaaca gctggctcag
     6841 gtacttagcg gctgcctgag ctgacttttc cgggtcgaat acatcattcc cacgcaggcc
     6901 catgtcgcgc gccgtgccat ccataaactg aaacagacct ttagcgccag cgcctgaaac
     6961 ggcgaactga ttaccgcccg attccgttat ggccacgctg cgcaataacc cctccggaag
     7021 ccggtagagc tgttccaggt tggtaaacat cggctgcatc catcccagca gttcagagcc
     7081 tgctttggtt ggctgtggcc gcttaactga ctggccgagc tgctcaggct catcatcacc
     7141 gaaccagccg cgcaccgttc ggcccacgct gcgaggatcg aatccccagt gctctttaat
     7201 ccagtcggcg gttccgttgg cgctgtctgt taccatcggc atcgctgacg gattttcgct
     7261 gccctgatta agcatctgtt tgccgatgct ggcggcatca gcccagcggc catctttaat
     7321 ggcgttgagc aggtcggcga tcatgttcag catcttgccg aactcaccca tttgcgaaat
     7381 gaagttgcta aaatcccact ttagggacca tgacttgggg tcaataccga gcaacttagc
     7441 cagcgctttt gtcaggtccg taacagtctt tttcaggtca ccaatcattt tgacggcctg
     7501 atcaatttca gtcttccatt tcccccagtc aatcaggctt ttgccacctt ctttccacgt
     7561 ctggtaatca tccaagagca gaccaagtgc aataataagc gtcgttatca tgcccaaggg
     7621 tgatgacatg aaagcggtat ttagcagccg ccatgcaacc aacagcccac tgaacaatgc
     7681 gataagctgc tgcgtggctg ggttcagctt tttgaaccag gcgatcacac cctcaacagc
     7741 ttgcccagtt cgccataaca cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat
     7801 gatcttcgtg atgaccgctt caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt
     7861 atcaatactg ccagcaaggc caccagctag gttagagcca atcttgtctt tcgcctgtcc
     7921 aagcgtcatc gtcagattac gcatggaggt catgaaaata ttggactgtt tagccgctga
     7981 ctcagcatta aaaccaatac gctttgccgt caacgcgtac tcagaactga gctgccccat
     8041 ccctctgcgc atcgccatca gcgtgttttc atcgatgccg agcatctgcg cgtattgctt
     8101 cgcgcggtaa tacggcatgt tgttgagctt ttggccaacg ccagtaaaga tggcagcagt
     8161 atcacgcatc tttccgctgg catcgcgggt ctggacacca agacggttca ggaacccttc
     8221 cgcccccgga ttgctacgca tgaagccagc caacccttcg agggaggaca tagccgactc
     8281 ggcgctggca ccggtttgcg atgcggcgta tcccagcgct ttgatgccct ggacactggc
     8341 ccccgtccgc tgggatgccc agtaaatttt atccagaccg ttcgcgatct gagtggtaaa
     8401 tccgacaatg ctcagcgctg cgcctttcac caccgcgccg accttcagaa cgttcgcggt
     8461 aacgcctttc agcacggctt caaacttatt agcgccagcc tgatcgatat cgaatcccag
     8521 cgaaacaagg aaatctttaa tcgtatctgc gttaccgctc attggccgct ctccatttat
     8581 ctacccgggc gtcgttatcc tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc
     8641 aaagatctac cgcaccgctg ttaaggtctt tctggtcaat atggaaggca agcgccgggc
     8701 gaagaataaa atcttcaccg cccggcaggc tgttgaaggt tattccgcta gcggggtggg
     8761 cgtctcgctg gtagggagtc cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc
     8821 caccagttgc agcatggtaa gcaggtcgat atcgtcaaac gccatttcgc catgctggca
     8881 gaccggcacc cagcctttca tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat
     8941 agcgtccacg tcgccatcac tcagatcgga cacagcattg gcaatctttg gcaggatagt
     9001 agccatcgcg ccttcggtgt ctttgctgct gatcttctcc tgaacgctcc ggaagtccga
     9061 aaccatcccg gccagaaccg gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct
     9121 gagctttgcg gtgcgatatt tcacgccttt aatttcgaat tccatgcgtt aaaactcccc
     9181 gagaagctgg tcaatcttgc cgcagtcgaa tacccaggcg acggttccgc cctctttagc
     9241 gttattgaaa tcaggctgtt tctggaatgc acacgaacgc gcagtagaaa tatcacccga
     9301 tgccgtgttg cgaatgacga tcacgttatt gccccaggtg gcagaggact ggctttgcgc
     9361 gttatacgcc agagacagct ttttattcac cggggaggtt ttgagtagcg tcaccgtaat
     9421 ggtgcctgac ttatcggcgt gcaggctgtg catcacctcg ccatcggcac cgatagtcat
     9481 ggtgtttttg ttgccgccca tggtctgggt gataccttcc tcagagttcg cagaaccctg
     9541 accaagatcg ataacgccgg tcggcccggt gagcgacgcg gttacatcga gaaaagaata
     9601 agttgccatt tatcgctcct tagcgaacca cgttgatctg cacatcggca taatgaactg
     9661 cgccagccag cttacaggcc acctggatta acggtgcttt gcgcgcttca cggtcggcct
     9721 gcgcctgttc ggacagcggt tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg
     9781 aattcagctg tccgataggg ccaccattcc acacgccagc agctaccaga ccgttcgtga
     9841 cggactgatc catggactgt tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct
     9901 gcggaatttt ggtggtgctg gtgtaaagca ggttatagag gttggtctga acgtagttct
     9961 gcaaccagtc gagcccgtgg cgctcgtcga agaagtcacc gttcgccatg acaccctgtt
    10021 gcaggatcgc cgtgtcgttg gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg
    10081 ccgatgcctg gctgactgtc agtgtttcat acccgacacc cggctcctgc ttaaacttga
    10141 gcgtaatcgc ggtattactg ccattgaaat taaccgtgaa tgcccggcca aatgcagata
    10201 acgcagcgta tttattaccc gatgaatatt gaataaaact gcgtgaatat ccggcggttt
    10261 tcagttttga tgccaaatca tcgctggatg cagtctgcag gcatttctca tcgcttgtcg
    10321 taatcgccag aatacggctt acagaagagg attcgatcgc cgcagccact ttcagccagt
    10381 ctgcatccgg aatatcttca tcgtctgcaa tccccagccc ataccatgaa gtataatcga
    10441 gcatggcatt cacagcctgc tccagcgtct caggcgtggc ctgttcgctg tctcccttcg
    10501 ttttcaccca acgaccaaca aaaacctcct gaggtttcgg tgattgtgag aaaaacacct
    10561 gcgcagcctt atattctggt gattccacgc caaaatcttt tccaatatct tccgcggcag
    10621 aataacggcg aatgcgctca cttaccggaa tgattgtgga cgggccgaga atgagtaatg
    10681 caccaaaatt tcgccctgat gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt
    10741 ttgatacagg caagccctgt gccatagctt aatctccgaa aaagatgact ggtgcttcca
    10801 ccagcgattt aataccgtac tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt
    10861 agcggcggac ccattgctga ttaataagtt cagggaagag acctgtgtaa tcgccaagag
    10921 acagccccag cgcattcagt gctgcgttgt tctgcggtac agatataccg tcacgaaacc
    10981 gggacgcata caccatcccc gccggaccat aaaacgaagc catacactca atcgtttcat
    11041 gccgccagag ctgagagcca tcatcggtct gtctggtgaa tgccggactg tcatcacctg
    11101 accatccgat aaccccaaac gcacaccagt tcgtttcaac cggtagcagt ggcggctgct
    11161 ctttctgcca gcgcgggcga accatcccgg cagacagacc ggaaacgtta cgcatccact
    11221 ggcttaacag cctgtcgagc gcttcgtcat aatccggatc gccactggtt ggtattaacc
    11281 atccgcgctc tgtactggtg ttattgctca accggagttc ccccatcaaa cggcatcaac
    11341 tcacaatgcg cctgaacgaa tccggcccca taagctgtat acgggtcgac gaaggtcaca
    11401 cgataatcac ggccctgata cgtcacgata tcggcatcac ggccagtctg tccctgcgtc
    11461 agtcgctcag tcgtcacaat cagaattgca ccgctgatta cctgccctgc ctgcatacgg
    11521 cggttttcca gagagcgatc aacagttacg actccggcaa actgcttttt aacttcactg
    11581 tcgctgccga tcccgtcctc atccaccgtt tgcactcggc gtgttaccca caaattgaag
    11641 tcgcaaaaat cggggtcaaa aagcacatct gttacatcaa gagtcggcat ctttatccct
    11701 cacaacatgg gtaatagctc tgcgatattg cccggtgtca attaatggtt tcgccagatc
    11761 ggttccggga gattcgccag caacacgccg agcaagttcc agtgttgccc ccttgcgccc
    11821 ccgacgagcc cgggcttcaa cggtgctatc agcaagcggt gtaaagccgg taatggtcat
    11881 gtaacgcctg acgccattcg cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc
    11941 cgccgcattt ccatcaagcg cagcctgcgc cgctgctttg agctgcggca ccgtctgttc
    12001 ctctacggat ttaacgccgg ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga
    12061 gccgtattcg ttgacgtaac cgatcccggc attaccaaac ggaacatcct cacgctcgct
    12121 gtcttcttcc gggatgccca ccagcacatc ctttttggtt agcgaccgga gcgcatccag
    12181 aatggcctga gcgttatcaa ccctcgttgt tacaccactt ttgaaactca tagctggcga
    12241 ccgcccgcac cgaacatcgt gatcagctga taaaattcag cgccatatcg ggtgttattc
    12301 cagaagcctg cgtcagggtt tagcgtcgcg ctggtgtcat agctgacgct taccttgtca
    12361 acggacttgg aggactgaac accattggtt gaaccgcccg ggccgccaac cagcattgcc
    12421 cgactatctg ccgcccatag cgtcatgtag tgagccacga acaacccggc aaagtacgga
    12481 aacaaatctt tgccggtgac gttttcgctc agcagttcat cggccagatt cagacgaaac
    12541 tggatttgcg cttcgggata tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa
    12601 tcacttaccg ctggcaggct ttgattcttt ggcatttttt acctcgttac gcgcgtctgt
    12661 ggctttgcca acggatactt ccgcgtgcgc acgagtgaac cagtgcgtgg caacgtcttc
    12721 ctccactgca tgacggcctt taacaaactc gcgccgcgaa ccgtcgggaa gcgtgagcac
    12781 aaacggggta tgtacgtgta ttactgcatt attttttgcc atcgggtcat ccttaatggc
    12841 cccgccaggg gggccatgtg gctgttaaat gccatcaacg tacgaaatgg tttctttgta
    12901 cactggctca accgcaccca gcttgccgta gtaggtcgca atctgataca gaccgcgata
    12961 ctggatagga acgctctgca acggcaccag cggatagcgg acgtattttt tatcgttggt
    13021 gtacgcaacc atgcgatcct tattccccac accacggcct ttcagccatt taaccgcgcg
    13081 gatattcagc ggaacaccgt tctggtgata gctgatggtg ttggtctgaa ggtacgtcaa
    13141 cagggactgg ttacccgcag atgaaacgat gatgctggac aacagagcaa actgctctgg
    13201 cgggatcagc aaatcacgcg gaaccacaga gtaaccggaa gcggcccacg catcagacag
    13261 cacctggtta atgcttgcgc ggatttcgtc cggtgttgag attgcccacg ttttggcagc
    13321 gttgttgaca ggcacgccgt ccagggtaac aaggcctttc aggtttaatg cggaatcgcc
    13381 aacatacacc tgctcgtcgt tgtccatctg ccatttgagc tgcatcccgt cgtacttctg
    13441 ggtgtcaatc gggcgaccta cctgctgagc tgctgccagc tctacaacgg tccagcccag
    13501 ttccatgccc cagagattca gcgggttacc ggatttgccg atatccacgt ttacgccagc
    13561 aatagcggtt gagtctttgc ctacccagtt tttgccattc ggatttgcac cagtacccgc
    13621 agcggcgaag ctggtattcg tccagctgga aatgtcatct gcgatagaga catcttcacg
    13681 caactgaata tcgcgggtcc aggtgtaccc cgccagtggc aggttcagag tctggtcgag
    13741 tcgctccagc tctccgatga gaaaggcacc agagctgtca acggttgcct gatcaaaagt
    13801 aatcattcgt ctgttcctta aatcttccag gaaatttctg cattgccgtt agcatcaccg
    13861 gcacctgtga attcagcgtt ggtcagcacc acatttttgc cactgactga cgtggacatg
    13921 aatccaccca gcggcacttt gatggattca tcagtggaga cgacaacgta taccgggtcg
    13981 ccttttttga tggtgctggc atcaaaatca gaaccgagat taacggtcac gtagccacgc
    14041 ttcatggcgt cgcccgggaa gttcttgcct gttcccacct ggcgaaccat gtccggctgc
    14101 gaagtggtcg gataagggcg cacgtagatc cccttcacct tgtctgcggt atcgccatct
    14161 gccagcggca cgaaaaaacc gtcagcatcg tatttaccag ccagcccata ggcagcaaag
    14221 gcgttatcgg atttaaggac caccggttcg acggttaagt cctgcgggcg agagatagcc
    14281 ccggcaatgc caacaggcat ccggtacaga tatgcagtca ttggattatc ctttgcggtt
    14341 agaccagaat tcagcgtttt gtttgttcag ggaagcgatg ctggtcatgc ccatgtttgg
    14401 acgttgtgca tcgcccgtgg tgctgcgggt gtttcgccct ttggcaatct cagacacagc
    14461 gttaaacgcc atatcgaccg attgcttagg caatttgcgg atatccgcat caccgacaac
    14521 ctggcgaacc agcgttttgt cagcagaagc cagaacctcg cgtttgaaca cggtcggttt
    14581 catcttacgg ctcagatcga tacccggaac gataacttcg gcacgccagg ctgagtcacc
    14641 agtaatcgtg gtttcctctt catcgtcctc gccgtcaccg gtcggattat cgtcaggttt
    14701 attatcgtta tcgcccgtgg catttccttc cagcttagcc agcagggctt tcagtaatgt
    14761 tttgaggtca tcatcactgt cgccggttgg acctccgccc atctctggta ctttgtccgg
    14821 tagcggttgc tgcggggaca ggttgatgtt gagattaacg ccctgcggca aatccccctc
    14881 atctcctgta accgatgcgg gagccgactc caccagttcg ttcatggtgt cagcgtcacc
    14941 cgttttgatg gccgtgcgca tgcgggtcca ccagcttttc ttttgatttg ccattgtgtc
    15001 tctgtctcca attgcacaac gatttccggc tctgcctttg gggacaagag ccacatggtt
    15061 tccggtaata tcgacctgct cggctttacc tggctcggtc tgctcgtact ccgcgtcata
    15121 gccgcacgac acttcacgca ggccatcttc gataagctga atggcgtttt cgtctttgac
    15181 gataaggtca gccagcatca aatcagagtg gtcacccgtc ccgcgccgca cattctgaag
    15241 atgcccgaca gcaagctctt tccagttctc gggatttacc agccgcacat tcccgttttc
    15301 atcttcagga tgcaggatcg tgatgctcat cccttcgaat gaggcgagcg tggccggatg
    15361 gaatacctgc tcaggagagc gcgttacgac tatctcaccg agcttgtcgg gtttgaggtt
    15421 tggcagatcg gcagcgccgt agagctgttt acccgttcga cctatcggca cgtctttgca
    15481 cagcagcgag ccgtcagcca gctgatagcg ggtttccccc agccgggtat tgaaaaaata
    15541 tttcatggtt tacctgcgat tcaggcgaga taagaatgag ggttgggaaa aacgatttct
    15601 ttataacagc gacaattcgg gagctcgcca gcgtgaccgg tcatgccatc aagcgttgga
    15661 ggtttgcccc attcgacaaa cttaccttcc atctcccgat gagaatgccg gacgtcgcca
    15721 tcttcggctg tacgccagat ataaccattc gagccgattg acagcgcacg cgcctgatcg
    15781 agcgcgccgg ttgcacgtcc aagctcggta cgggcgataa ggttcgctcg tgagcgtgac
    15841 acgtcaccgg acgctgctat ctctttcgcg aatggttcag cgcggccacc agccacaacg
    15901 gcctcgatgg ccttgttctg aatgtcatac acccgatcgg cggcctcaag aggcagtgac
    15961 ttgatatact taatttgctc ggcgacgatg gatttcatca cctggcctac cggggcgcgg
    16021 tcgaccatgt tgcgcagctc tgcgctgatg ttccggctgt gctgacgcca ctgcttttca
    16081 ttctggcgtg caatgtcggc ggtaaagttc tcagcaacct tcgtcgccca gggggtgatg
    16141 atttcgctgt agcgctccag cgcatccatt atttcggtaa cgctatcatt tgaaccatcg
    16201 tagcgcccat ttacgatatc cccgaccgcc cgcgctatct gccgtaggct cgttcgatat
    16261 cggatctccg cctggcggct ctggcggttt gtcgccaagt tcgccgatgc ctggcggcgc
    16321 ttcgtcttcg gcattctcga tatcctcgtc ggtaatggat gccccgatgc cggtgacgtc
    16381 agagttttcg cgcaggtcgg tcatcgccgc cttacgcgtc atcaatccgt cgcccagcgc
    16441 ggtactaatc gcgttggtgg tgtttacggc caccgttgat cggtcaacgt ctgacatttg
    16501 ccatagcggg ttaaactcaa acgtgaaatc gtccggcagc ggctttccga gttccgagcg
    16561 gtgcataatg tccagtatcc ggcgcatcgg cagccgtaag cggcgctcct gcaatgagct
    16621 cacccggtcg taatagttgg cgaggtctgc atcaccagta gagaagcctt tcggggattg
    16681 accgaacaac cgcaccagcg ggataccaac ggcaccgcta atctgctcag caaactgcga
    16741 aagaatgtca tccagaccac taaagctgta ctggtgggtt tcgaacttat cccgcgagtc
    16801 catgagcgtc ataccttcat tgctctggaa ctggcggatc aggtcgatgt tcttcagcaa
    16861 cgcctcgaac gccgggcctc caagcgcgat aagctcgcgc aacttctcca cgctataggt
    16921 acgcagatgt gctttgtaga ccagctgcgc cgcgccgaca gtggcgctgt cgaacgcagt
    16981 aagccgatcc cagatacgct ctacaaccga cattccccat tcgttttcgg tcatcttctg
    17041 ctggaatggc agcgtcaccc cgtcgaagcg aatcaggcgg ctgtgatgga tgcgccaggc
    17101 cgggatgccc gttgcagtgg tcaccacgtc gtaaaactca ggtttgccga gatctggccc
    17161 catctcttta atgcggcggg tcaggaccgg gttaatcatc cagcggtcga gcgggagaat
    17221 gcccttaaac ttgccttctc caatggtttc gagccgcagc ggggtcattg gtgcctgccc
    17281 ctcgatcatg atgaagccga ccgcgccgcc gtagaggcga gaccatttca gcacgtcgtt
    17341 cagcgcatcc cagatttgca actcatccag ttgtgattcg agaatgccac gatcttttgc
    17401 atcaatttcc gaagtgatgc gaatgccttt gcgggtcata tcatccggga tagcatcgac
    17461 cgcttcgccg ataatccagg acgaacgata ggaccattcc accagcatgc ggttacgact
    17521 ggtgaaatta gcccggtagg tggatgctga gtgctggtta ggtgtctgca tccccacgcg
    17581 ggcaacaaag ttctcatagc catcagcggt ggcctgtgcc gttcgccgcg tggcttgttt
    17641 gtttcgtgcc atcaggcctg tctccctagc agctcccaga tgttcagggc tgaattcatt
    17701 ggcgcgtagc tgatcatcac cgagtcggca aggtttggcg accgggttcc atcaggctgt
    17761 ttatcaataa cgatttttcc cacaccatta atggaatagg tcggctgcga aagctcgatg
    17821 atgagtttat ctttgagtgc catgctactg ctgattgaga tgatttcgtc cgggttgtaa
    17881 tccatacctt caaccacggc gcgccaggta ttctgaaaaa gtttacgtaa ccgccaccag
    17941 ctctgggctt tggcgttagc gaagaagtcc ttgttcaggc gggcggcctg tccgttgtca
    18001 ccgcgcaccg cttcgtcgtc cggatcaaat accgcgccac tacctcgaaa cggtgtggcg
    18061 agtattgacg gtcggcgcgc agcgttacgc agttcgttga tagcgcgtgc atcgccgcga
    18121 acgccagcgc ccaacccgtc ctcgtcaaag cgaaactctt cgaggttgtc ctgttcgcaa
    18181 aagccgaaaa ccttctcgac ggactgataa atgtcgctgc ccacaccgga ccattcccgc
    18241 acattctcca ggaggaagcc atgacgggtg gaaaaggaat ttttgtccct gccttcgtcg
    18301 gcgacgtcca tcgcgcccag tcgcttgcct gttggctgga tgcccagctt gatatgcgcg
    18361 tcgacagcag cctgtaccca ttcggatgga atcaggacgc cttccgctga tgcactgtag
    18421 ttcaggtcaa gttcctgtgc caccaccacc ggattgtcga ttttctcgca ttccctgcga
    18481 taccactctt catccttgcg aggatcatcc cgccagtgga atgtgaatac cggtatcttc
    18541 ccgccatgac gcttctgagc gaacgggttc gccatgccgt taactgaact caggtcaata
    18601 cggcaacgcg tcgtttgtga caacgccgca tcaatcagca gaggacgctg aaggaatgca
    18661 gactcatcaa ccagataaag cgtggtacgg tcaccacggc caatattatc gccagcctca
    18721 cctttgataa cggcaccagt ttcaggaaac tcaacgcgca tgtatggtgc gtgcttcttt
    18781 tcgtcccacg aacctcgaaa ctctacaggt agtgtttcca cgaacttgcg cgccttccag
    18841 aacaatgctt tcgggtcacc ggtgctgtcg acgtattcct ctttacggga gccgaaaccg
    18901 ataaccattt ctttgttgaa gagacaaagc gagcatgcca gcccgatcgc ggtccaactg
    18961 agccccattt cacgggattt ttcggtaata ccattctccc gattacccca gcgttccata
    19021 atccagtgga tccactcctc ctgcttaggg aagagtaaaa acggaatggt caccggcagg
    19081 ccataatcaa tattacgcgg gtccgttgtc atgccccagt cgatgatgaa ctgagccgga
    19141 ttggttcggt aaaactgttt tagtgcaggc aatatttcag gattctggcg aatgcgctgt
    19201 aggcgttcca tccgccattc aaaaaccatc tggtaatcag gatgtttaaa atcgaagggg
    19261 aatggtaacg gcatacttag cccatcattt ttctatacgc ctctgcagcc tgctccggcg
    19321 ttaagttggt aatttctgtt ctgactggtc ctccgtcagc accagtcact tcatttttga
    19381 cattgtcttt aaacgcctga acagaaacat gacgcccgag caactcaagg tttttaacct
    19441 tatcaggcca tttgattttc ttcaggagag cagcactatc tgctgatgcc atctcgacga
    19501 catccattcc tgatagcgtt gtacgccaca ccttaggcca gtctttaatc tgcttcagtt
    19561 cgccattggc aagcagaatg tcaagcacgt ccatctgatc gatttcagtc agccgtcgca
    19621 atacataggc ggcgtctata cctacctctt cattgcgttc agcctttagc tcagcaatgc
    19681 gatctgctat gtcaggtttt gacaggttct cgctaccagt ggcgcgagca gttttctcgc
    19741 tgtaacccgc cctgatagct gcctgagtgg cgtttaaatc ttttaaatat tccctgcaga
    19801 acagttcctg cttaggtgtg agctgtttca aatcacacct ccgaaacatt gctccaattt
    19861 ctatgattaa cgatgtcagc aatcatggta gtggatacgc catatctctt tgctaaagac
    19921 ctaagcccat tacctacact cccgcgcaca tactcgcggc gaatgcagat gacatcgcac
    19981 tcttttaatt tcgccattcc gttcttttca cctgaacggt cagggtatga gtttctgcct
    20041 cgccttgacc tgtcacgatt attgtcagcg acagtaccta taatcagatg gtcaggattc
    20101 acgcatgatg gattatcgca tttatgccta acaacaaggt ccttga
//
LOCUS       DADLDT020000079        19748 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000079 DADLDT020000000
VERSION     DADLDT020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19748
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.079"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            269..1366
                     /gene="mltA"
                     /locus_tag="JQH22_002683"
     CDS             269..1366
                     /gene="mltA"
                     /locus_tag="JQH22_002683"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFB2206949.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1605..2411
                     /gene="tcdA"
                     /locus_tag="JQH22_002684"
     CDS             1605..2411
                     /gene="tcdA"
                     /locus_tag="JQH22_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFB2206950.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2462..2905)
                     /gene="csdE"
                     /locus_tag="JQH22_002685"
     CDS             complement(2462..2905)
                     /gene="csdE"
                     /locus_tag="JQH22_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFB2206951.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2905..4110)
                     /gene="csdA"
                     /locus_tag="JQH22_002686"
     CDS             complement(2905..4110)
                     /gene="csdA"
                     /locus_tag="JQH22_002686"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFB2206952.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4302..4529
                     /locus_tag="JQH22_002687"
     CDS             4302..4529
                     /locus_tag="JQH22_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFB2206953.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4880..5797
                     /gene="gcvA"
                     /locus_tag="JQH22_002688"
     CDS             4880..5797
                     /gene="gcvA"
                     /locus_tag="JQH22_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFB2206954.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5816..6211
                     /locus_tag="JQH22_002689"
     CDS             5816..6211
                     /locus_tag="JQH22_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFB2206955.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6204..7304
                     /gene="rlmM"
                     /locus_tag="JQH22_002690"
     CDS             6204..7304
                     /gene="rlmM"
                     /locus_tag="JQH22_002690"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFB2206956.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7348..8079)
                     /gene="fucR"
                     /locus_tag="JQH22_002691"
     CDS             complement(7348..8079)
                     /gene="fucR"
                     /locus_tag="JQH22_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFB2206957.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8137..8559)
                     /gene="fucU"
                     /locus_tag="JQH22_002692"
     CDS             complement(8137..8559)
                     /gene="fucU"
                     /locus_tag="JQH22_002692"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFB2206958.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8561..9980)
                     /gene="fucK"
                     /locus_tag="JQH22_002693"
                     /pseudo
     CDS             complement(8561..9980)
                     /gene="fucK"
                     /locus_tag="JQH22_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10089..11864)
                     /gene="fucI"
                     /locus_tag="JQH22_002694"
     CDS             complement(10089..11864)
                     /gene="fucI"
                     /locus_tag="JQH22_002694"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFB2206959.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11897..13213)
                     /gene="fucP"
                     /locus_tag="JQH22_002695"
     CDS             complement(11897..13213)
                     /gene="fucP"
                     /locus_tag="JQH22_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFB2206960.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAELFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13760..14407
                     /gene="fucA"
                     /locus_tag="JQH22_002696"
     CDS             13760..14407
                     /gene="fucA"
                     /locus_tag="JQH22_002696"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFB2206961.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14435..15583
                     /gene="fucO"
                     /locus_tag="JQH22_002697"
     CDS             14435..15583
                     /gene="fucO"
                     /locus_tag="JQH22_002697"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFB2206962.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15636..16391)
                     /gene="xni"
                     /locus_tag="JQH22_002698"
     CDS             complement(15636..16391)
                     /gene="xni"
                     /locus_tag="JQH22_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFB2206963.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16502..17869)
                     /gene="sdaB"
                     /locus_tag="JQH22_002699"
     CDS             complement(16502..17869)
                     /gene="sdaB"
                     /locus_tag="JQH22_002699"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFB2206964.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17927..19216)
                     /gene="sdaC"
                     /locus_tag="JQH22_002700"
     CDS             complement(17927..19216)
                     /gene="sdaC"
                     /locus_tag="JQH22_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFB2206965.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4873 a         4837 c         5209 g         4829 t
ORIGIN      
        1 tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg
       61 tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt taccccgatc
      121 acggatttag catgattttg aacaaaaaca aaccactaaa gcctattttt tgtgcaaaat
      181 ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc
      241 cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt accttcttat
      301 gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca
      361 atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag atgccgttgg
      421 cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta attcgtcacc
      481 gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc tgcgcgcagg
      541 cggtgatacc cgcaatatgc gccagttcgg cattgatgct tggcagatgg aaggtgccga
      601 caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg cgcgccatac
      661 ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc
      721 gtctcgtgcg gagatctacg ccggggcatt gggcgataaa tacatcctcg cttacagcaa
      781 ctctctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg actttggtga
      841 tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct atcgcagcat
      901 tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat
      961 tcgtcattgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg aacagaaccc
     1021 gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc
     1081 gctggttggc cgtgcttctg ttgcctccga tcgttcaatt attccgccag gtactacctt
     1141 gctggcagaa gtgccgctgc tggataataa cggcaaattt aacggtcagt acgaattgcg
     1201 tctgatggtg gcgttggatg tcgggggcgc aatcaaaggc caacacttcg atatctatca
     1261 agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg gacgggtttg
     1321 ggtgctgaaa acggctccgg gcgcaggtaa cgtctttagc ggctgatgtg atattctgta
     1381 tgccgttttt acggatatga cagggtgagg ggtaacctca catcctgatt attgactaag
     1441 tgcgcgttgt tcataccgga tgcggcgtga acgccttatc cggcctacaa atcatgcaaa
     1501 tccaaaatat tgcaggagtt atgtaggcct gataagcgta gcgcatcagg catttatgcg
     1561 tttagggtga gggataacct cgcccttttt aattctgagg tgctatgtct gtggtaatta
     1621 gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct gtatggtgaa aaagcgttgc
     1681 aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg tggtgtcggt tcctgggcgg
     1741 cggaagcgct ggcgcgcacg gggattggcg caatcacgct tatcgatatg gatgatgtgt
     1801 gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga taacgtcggg ctggcaaaag
     1861 cggaagtcat ggcggagcgc attcgccaga ttaacccgga gtgccgtgta acggtggtgg
     1921 atgatttcgt gacgccggat aacgtagcgc aatatatgag tgccggttat tcgtacgtga
     1981 ttgatgccat tgatagtgta cggcccaaag cggcgctgat tgcttattgt cggcgcaata
     2041 aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat tgacccgacg cagattcagg
     2101 ttaccgatct ggcgaaaacg attcaggatc cgctggcggc gaagttgcgc gagcgcctga
     2161 aaagcgattt tggcgtagtg aaaaacagta aaggtaagct cggcgtggat tgcgtgtttt
     2221 ctactgaagc gctggtgtac ccgcagtcag acggtacggt gtgtgcgatg aaagccacgg
     2281 cagaagggcc gaagcggatg gattgtgcat ctggatttgg cgcggcaacg atggtgaccg
     2341 ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa gatgatggcg aaagcggcgc
     2401 gtcagggtta aacaatcagg tcggatgaga cgcgaacgtc ttatccgacc cggttctgtg
     2461 cttaaacctg cttcgtcgca gcgataatcg cctcgcttaa cgcatttaac ccctggctgc
     2521 gtgaggcgct aagctgcgca cgtaatccca gctcatcaaa caatgccagc ggcgactgtg
     2581 cctgtaattc ggcggcggtt ttcccctcaa cggcagtcaa caacaccgcc aacaggccgc
     2641 gcacaatgcg cccttcgctg ttgccaaaga agtgcatttt gccgttttca gccacggtat
     2701 atcccagcca gacgcggttt tcgcatccgg caatctcttt agcctgcgct tttaactcgt
     2761 ctggcaaagc cggaagctgt ttccccagca tgatcaactg gcgatattta tcttcccatt
     2821 gcgacaacgg tgcgaaggta ttgcgtaacg tttctgcggt tacggttgtg ccgaacggat
     2881 gtccggcgaa ttgcgggttt gtcattaatc caccaataat tccagcgcgc ggtcaacggc
     2941 attcaccagc gcatccacat cactctttgt attatatggc gcaaatgagg cgcgcagtgt
     3001 gccggttacg cctaattctg ccagtagcgg ctgagcgcaa tgttgcccgg cccgcaaggc
     3061 aataccgtac tccgccagta gcgtcaccat atcgctgtga tgaacgccag caaaatcaaa
     3121 ggccagcagg ctggaatcct ggcagcggaa tgaacgaaag cctggacgtt tcgccagcgc
     3181 ttcttctgca agcgttgcta agctacggct ccagctttcg gcctggttga tatcgtaatc
     3241 tgccagccat tccagcgccg cgcttaatcc tatgacgcca gcgacatttg gcgttcctgc
     3301 ttccagtttc cacggcgcag attgagtggt gaaaccgtca aaactcactt cgtgaatcat
     3361 tttgccgcca cccagccagg gcgacatcgc ttccagcagt tctgatttgc catacagcac
     3421 gccgataccc gtcgggccat acagtttgtg accagagaaa gcatagaaat cgatatccag
     3481 ttgctgaaca tccgcgggga aatgcaccgc cccctgagca ccatcaacca tcaccaccat
     3541 cccggctgaa tgagcaaagg taatcgctcg cgccagatcc gggcaaccgc cagtcacgtt
     3601 cgacatctga cccaacgcca gaatccgact acggggagta atcagctctg gcaacaaatc
     3661 gacatccggc agtcgctgcg cattaagcgg caatttcacc actttggctc tagtttgttg
     3721 ggcgaccatc agccagggga cgaggttagc gtggtgttct gccacgctga caataatctc
     3781 atcgcccggt tgcagacgcg gacgcgcata gcattgtgcc accatgttga tggattcagt
     3841 ggtgccgcgc gtccagacga tagttttatc atccggtgca ttcagtaatt gcgccacttt
     3901 ctctcgtgca gcttcataac gcgcggtcag gcgttgggct tcggcaaact ggctgcgatg
     3961 gacgtttccg gcgctcaggc tgtaaaactg tcgagtggct tcaaccacgg cttcaggttt
     4021 aagcgcggtc gcggcgctgt cgagatagac gcccgcatcc tgtagtgcgg gaaactgggc
     4081 gcgaaactgc gcgggattaa aaacgttcat ggtactcctc ggcttgatgc accgatcgtg
     4141 acgcaaaatt cactttgata caaggagagt gctaaccatc agaattatct ggatatcctg
     4201 gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc gttaatttta gcctgttaat
     4261 caatactatt aagatttatt aatcgtttta aggagaagat gatgaaaaag actgccgcaa
     4321 ttatttctgc ctgtatgctg acttttgccc tgagcgcctg ttccggttcg aactatgtga
     4381 tgcacaccaa tgacggacgt accatcgtct ctgacggcaa accacagact gataacgata
     4441 tcggtatgat ttcgtataaa gacgctaatg gcaacaaaca gcagatcaac cgtactgacg
     4501 tgaaagagat ggtcgaactg gaacagtaat tcgcgatcgc aaggtaaaaa aaagcaccgc
     4561 aattaggcgg tgctacatta atcactatgg acagacaggg taaatgtaca ggaagtgaaa
     4621 aaaggtagct ttgctaccat ggtctgaatc gcagaccaat tgcaaacaca acaacacaac
     4681 atcacaaccg taagccaaaa gttcaccaga atacgcattc cgataaaact tttcgttccg
     4741 gctcaggaag tgccgccact ataggtattt gctggtagaa gctcaacgga caatttataa
     4801 tggctcagat taaaaaaact aataggttac acagtgtgat ctaattgtta aattcattta
     4861 acatcaaagt ttaaaagcca tgtctaaacg attaccaccg ctaaatgcct tacgagtttt
     4921 tgatgccgca gcacgccatt taagttttac tcgcgcagca gaagagcttt ttgtgaccca
     4981 ggccgcagta agtcatcaaa tcaagtctct tgaggatttt ttggggctaa aactgttccg
     5041 ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa agctatttcc tcgatatcaa
     5101 agagatattt tcgcaattaa ccgaagcgac gcgtaaactc caggcccgta gcgccaaggg
     5161 ggcgttgacg gtcagtttac tccccagttt cgccattcat tggttggttc cgcgactttc
     5221 cagctttaat tcagcttatc cgggaattga cgttcgaatc caggcggttg atcgtcagga
     5281 agataagctg gcggatgatg ttgatgtggc gatattttat ggtcggggca actggccggg
     5341 gctacgggtg gaaaaactgt acgccgaata tttattgccg gtgtgttcgc cgctactgct
     5401 gacaggcgaa aaacccttga agaccccgga agatctggct aaacatacgt tattacatga
     5461 tgcgtcacgc cgtgactggc agacatatac ccgacagttg gggtttaatc atatcaacgt
     5521 tcagcaaggg ccaattttta gtcatagcgc catggtgctg caagcggcta ttcacgggca
     5581 gggagtggcg ctggcaaata acgtgatggc gcaatctgaa atcgaggccg gacgtcttgt
     5641 ttgcccgttt aatgatgttc tggtcagtaa aaatgctttt tatctggttt gtcatgacag
     5701 ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg atcctggcga aagccgctgc
     5761 tgaacaagaa aaattccgct ttcgttatga acaataattt acgtagggta cgaccatgac
     5821 cagccgtttt atgctgattt tcgccgctat tagcggcttc atttttgtgg cactgggcgc
     5881 ttttggtgcg catgtgttaa gtaaaaccat gggggccgtt gagatgggct ggatccaaac
     5941 cggtctcgaa taccaggcgt ttcatacgct ggcgatctta ggtctggcgg tggcaatgca
     6001 gcgtcgcatc agtatctggt tttactggag tagcgttttc ctcgcgttag gcacggtgct
     6061 gttcagcggc agcctttatt gcctggcgct gtcccatctg cgtttgtggg cgtttgtcac
     6121 accggttggc ggcgtgagct tccttgcggg ctgggcgtta atgttagttg gtgctatccg
     6181 tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt gctgtgccgt ccgggctttg
     6241 aaaaagagtg cgccgcagaa attaccgata aagccggcca gcgggaaatt ttcggttttg
     6301 cccgcgtgaa agagaatgcg ggttatgtca tttatgaatg ttatcaacct gatgatggcg
     6361 ataagttaat ccgtgagctg ccgttcagtt cattaatttt tgcccgccag tggtttgtgg
     6421 tgggggaact cctgcagcat ttgccgccag aagatcgtat tacccccatt gtcggcatgt
     6481 tacagggtgt agtagagaag ggcggtgaac tgcgtgttga agttgccgat accaacgaaa
     6541 gcaaagagtt gctgaaattc tgccgtaagt tcactgttcc gctacgcgct gccttgcgcg
     6601 atgctggggt gctggcgaac tatgaaacgc cgaagcgtcc ggttgtgcat gtattcttta
     6661 ttgcaccagg atgctgctat accggttact catacagcaa caataattcg ccgttctata
     6721 tgggcattcc gcgcctgaaa tttccggcag aggcgccgag tcgttccacg ctcaaactgg
     6781 aagaggcatt tcatgtgttt attcctgcgg atgagtggga tgaacgcctg gcgaacggga
     6841 tgtgggcggt ggatttaggc gcttgccctg gcggctggac ctaccaactg gtgaagcgca
     6901 acatgtgggt ttattccgtc gacaacggcc cgatggcgca aagtctgatg gataccgggc
     6961 aggtgacgtg gctgcgggaa gacggtttca aattccgtcc gacacgcagc aatatctcct
     7021 ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc ggcattgatg gcgcagtggc
     7081 tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa actgccgatg aaaaaacgct
     7141 acgaagaagt atcacacaat ctggcgtata ttcaggcaca gcttgatgaa catggcataa
     7201 atgctcagac tcaggcgcgg cagttgtatc acgatcgcga agaagtgacg gtgcacgtcc
     7261 gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg ataacaggca aggccggggc
     7321 gtcgtctccg gcctgctacc ctttccgtca ggctgttacc aaagaagttg caacctggcg
     7381 ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg atgcgggctt cccctgaacg
     7441 attaaattta cttttatcaa tcaataacaa cgattgcgcg gcacgtttta atagcatcga
     7501 tttgtaatca gcgttgatcg cattggagtc ccacagtgcg ccgctgctat cgatcccttc
     7561 acaagaaaaa ataaacagat cgatttccag cgatttaagt tgggaaatca gcgagggatt
     7621 gacgtaacag ccatatttgc gctcaagtgt gccgccggaa ctgatcagtt gaatgcgttc
     7681 gcgtttaccg agttcatggc aaatcggatg gctattggtg aagacctgaa tgttgatgtc
     7741 aggcaactgg cgtgccagat accagcaagt tgaactggcg tctaaggcta tcaccatccc
     7801 ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct ttatgcgcat aatggctttt
     7861 cagcctgatg tgaaagggat cgccactgtc ttgattttga cggtggatat atttagcgcg
     7921 tccatgattg cgcagaattt taccctgcgt ctgtaattca ttgagatcgc gacgaatggt
     7981 ttctttactg acctttagct gttcagagag agcttccgtg gtcaggctgg tatggttcag
     8041 cagcaggtcg actatcgctt gctggcgtgc cgctttcata tttttcaccc catcattcga
     8101 taaccgcccg cgtcatgcgg gcgtaccggt atgagattac ggtgttaccc ctttttttaa
     8161 aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg atcgcaaagg ctttttgcgc
     8221 ccgttcataa aacgcaaaac gattgatgcg gatgatgtca ggacacgggg cttgtagtga
     8281 aagcgcatta cggtaacgtc gttctacttc aggatcgaga gtgtcaccct ctaccgccgc
     8341 catcatcacc agcagcgggg cataactgtc cagctcaaat aacgggataa tcgcctggag
     8401 caagtcgctc accaacaggc catcagcgcg gatcacctgc ggttccatcg aatgggcggg
     8461 aaagtgagca tcggaaaaaa taatttcatc tccatgtccc atctctgcca gcactttcaa
     8521 tagttcggga gaaattaacg gcgaaattgt tttcagcatt tcacacttcc tctataaatt
     8581 caggttcagt ttgcgggtag aaataacggt actgataatg aatctgtgca cgggcttctt
     8641 ccgggctgtt aaattcccct acgccatacc aaccgaacag cgcagctcct gcgacggtcg
     8701 tttcggcatc gtcgagaact tttaccggaa tatcaagcat attggcttta atctgattcc
     8761 acaatgtgtt gcgacttcct ccaccgacta acaataattc ataggcttta aagtgcccga
     8821 ttttttccag catctgtaga ttgcgctgta attgcgcagt taacccttcc agcgccgcgc
     8881 gatagaaatg cccccgcgtg gtattaagcg tcactccttg ccagccagcg ttctgacacg
     8941 acaataaatc acactgcatt ttcacaccat ccgcgccagg tgcgatcaga cgagcttctt
     9001 caatcaacat ttgccagggt gtttcagccg tccagaacag ttttctcacc cattccagca
     9061 cgccggatgc cagccattgc atacctgggt tatacaacca tgcctggcta tctagttcgc
     9121 aggtggaacc ggcgtactga cttaacagcg aagtatcaac ctaggcgctg cgaaccatta
     9181 aaatttccca tgtaccggag gagagcacgg gttcattttg ttcggcacca gcgccaaaaa
     9241 gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat gccgacgggt aagccgagca
     9301 ttgctgcggc gttgttctgt agcgtaccaa tctgttcacc cgcttccacc agacgaggga
     9361 agagtcggcg tggaataccg gtggcttgta aaatttgcgg actgaaatcg cgttgctgga
     9421 tatccagcat ctggctggtt ccggccatcg tgatatcagt agtgaattcg ctggttaaac
     9481 ggtggttaat cagcgacgaa atgaagagcc aggcgtgcgc gcgttccagc agttgtggat
     9541 gattttcttt caaccacacc aacttatata acgtattgaa actaaaggct ccgacgccag
     9601 aaatagcctg caaccgctgt gcggagatta accgttcaat attgtccata accgctgctg
     9661 ttcgcggaca tttccagcta atgatcggat agagcagatt gccttgctta tctaccagag
     9721 cgccatccac actaaaggtg gtgacggcga taccgcggat gtggcattca gtcagttcac
     9781 tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat ggcagtctaa agaccactgg
     9841 tgccaggtgt tgttttccat cgcgatatcg ctggcattag gcgttgaggc gcgggcaaca
     9901 atttttccct gccgattaac cgcgatggcc ctgacattgg tcgcgccaca gtcgagtacc
     9961 aggataactt cttgtttcat aatcgctcct gcaatatagc cggataacat tgtttatccg
    10021 gctaaccact cttgcttgtt caggcccggt agccggagcg accgggcatc acatcagggt
    10081 gtaatgtatt aacgcttgta caacggaccg tagttctggc aagcgcggta atcctggcct
    10141 tcaatatcca tgccgtgcgc agcccaggca gaaggacgat acactttggt ctcttcaacg
    10201 ttgtgcatac ataccgggat acgcagcatg gaggcaaggg tgataaagtc tgcgccaacg
    10261 tggccgatgg tcagaactcc gtggttggca ccccagttcg ccattaccga atacacatcc
    10321 gtaaacggcc ctttaccagt gaggcgcggt gcaaaccagg tggttggcca ggttgagttg
    10381 gtgcgtttgt tgaggatgtc atgcacatcc ttcggcaatt ccacgctcca gccttccgcg
    10441 atttgcagta ccggtcccag gcctttgatg atgttgacac gagtcatggt gaacgggacg
    10501 ccgccttcgg taaggaagcg ggaagagtaa ccgccgccac ggaagtattc gtggatcgcc
    10561 gggcaccatt tggtagcggc gaggcaagcg tcagcctctt gctgagagat ttcccagtgc
    10621 ggcttcatcg tcgggttacc ttcgctgtcg cgttgtttac aggaaccgtc cagcgcagca
    10681 gaacctgagt tgatcaaatg gatgatgccg tgttctgcca gtccgtccag tttatgcccc
    10741 gttacacgct caattgcttc tggtgaccag taggtacgca catcggcaaa tacctgagcg
    10801 gtgccggtga gctggtgacc cattagcatt gccacgccgt taagactgtc gttttcggtc
    10861 gccacgacaa agggttcgcg cacgccattc cagtcaaatg aactgttgag gatcgcttcg
    10921 gcggtgtcac cattgggata ttgatcggtc cagtgacgtt gcccctggaa gcccgcagcg
    10981 atggcgttgt agccaagtga ttcttccacg cgaccaatat cggccagttt gctgttgcct
    11041 tgcatcatgt cgcggataca catcgccatc agtaaacttt cgcgcagaac tgcgcggctt
    11101 tgctcggcat tgcgttgata ctgtttgtta ttttcatctt cgtcatagcg gaagttttta
    11161 tcagcccagg ccagtgccat ttccagttcg gcttcgtcgt aaatcttctg atcgatacgg
    11221 cgacgcagtt cggtcatatc caccgcctgg actttcattc ccagccagga ttcaaagaag
    11281 ttgtgatcaa caatagaacc ggcgataccc atcgaaacgc cgcccagcga cagatagctt
    11341 ttacctttca tgctggcgac ggccaaaccg gcgcgggcaa agcgcagcag tttttcttca
    11401 acatcggcag gaatcgatgt gtcatcggca tcctgaacgt catgaccgta aatggagaat
    11461 gccgggatgc ctttctggct gtgagctgcc agagccgctg ccaggtaaac agcgccgggg
    11521 cgttcagtgc cgttaaagcc ccaaatggct ttcgggcggg ttggatccat gtcgatggtt
    11581 tcactgccat agcaccagca aggcgttacc gtaatggtga ggcctacatt ctggctgctg
    11641 aatttttctt cgcaagcagc ggcttcagcc atacccgcga tacaggtatc ggaaatgaca
    11701 cactcgacgg cagctccgca ggcatggcgc agtttctcgg tcagcagtgc ggccgtagct
    11761 ttcgccatat tcattgtttg ttcttcaagc gactcacgaa cacccatgcg acgaccgtca
    11821 ataaccgggc ggataccaat tttcggtaag ctgatttttt tcattacaga ttcctcactt
    11881 tattcggaaa atatgttcag ttagttgccg tttgagaacg gaaacgggca aagataaaga
    11941 tgaccgcgaa gcagagtgcg gggatcagtt cagcagtggg gatgttgccc gccgcgtcac
    12001 tgacaaaacc catgaccgga gtgacaatac cgccgccaat aatggtcata acgatgaagg
    12061 acgaaccgta tttggtgtcc tggccgagat tcttaatgcc cagcgagaag attgttgggt
    12121 actgaatcga cataaaggcg ctgcataaag tcagggctat taagcccaca tgaccgccag
    12181 cgaaggctga gatcaggcac agtgccatag cgattaatgc gtaggcggcc aggactttgt
    12241 gtggtgcgaa gcgactgatg agccaggtac cggtgaaacg accaataaag aagcacacca
    12301 tggttccggt taaatagtta gcggcaaagc ctgcagtcat acctggaatt tcttctacag
    12361 cgtagcgaat caaatagctc cagcaggccg tttgtgcgcc gacatagcag aattgcgcta
    12421 ataccgccca gcgccagtga cgaatacgcg ccagacgaga aagcgatgcg gagaacgatc
    12481 cttgtttggc gtcactgtga ttatcactct gcaatgccgg gaatttcgtc agcatgatca
    12541 gcagggcgac cagtaacacg atagccacga tgatcatata aggtgtctgt accgataata
    12601 ccaggctgtg tttatacgca ctcaattgct ctggagacat tttatcgaga acgtcttgcg
    12661 attgatgtgg cacgttagac aaaataagac tttgcccaaa gacaaccgcg ataattgcgc
    12721 caaacgagtt aaatgtttgc gcaagattta agcggaagtg actactgctt tccggcccta
    12781 ataccgtaac aaaagggttt gcggcagttt ccagacaacc taatccggct gcaataataa
    12841 ataggccaac taaaaataag gtgtagttca ttatttctgc agcgggccag aataattcag
    12901 cgcccaaggc atataaaaat aatccggtaa taatccctgc tttataactg agttttttca
    12961 tcaatatccc agcagggatt gggataatga aataaccaaa gtaaaaggcc gattggatca
    13021 ggccagcctg gaaatttgtc agcgtaaaag cctgctggaa ttgaggtaat aaaatgtcgt
    13081 taaggttatt ggctaccgcc caaagaaaaa acagtgagca cagcagcgcg aatggaataa
    13141 tgtaacttct gctttgccct gcatctttat ctaccgcacg gtaactctgc gtttgtattg
    13201 atgtgtttcc catagcatcc tcttaggttc agaagcttaa gcacgtaatc ggttatgttt
    13261 tcaaaagtgc aaaaccagcg agttcgggct tcatatcggt gaatatttgc ctgtctgaaa
    13321 tgaactcatg gattcatttt caaataaaaa acgctctgta atatgacggc ggtcacactt
    13381 attagctgga aggtgacttt atgtgactac catcacttta ataatcgaaa tttcatgccc
    13441 gtttcagaaa ataaatcata aaaaacacaa accagatgac cgtattaatt gtttgtgccg
    13501 aaaacgggca tttctggcga ggggaaatgg aaaaatgacc gttttgataa atatttttaa
    13561 gagcggtcat ttttaacggg gttataaaaa gagaacttgc agttatttac tgtgattagt
    13621 tgagctaggt cacaaaatat aagcttgtga atttctttct tgttaatcag gaagaagatg
    13681 gtgggtaatt aaacggctaa ttcaatagtg tgaaaggaac aacattattg ccctgttttg
    13741 aatcagagag aggtagctaa tggaacgaaa taaacttgct cgtcagatta ttgacacttg
    13801 cctggaaatg acccgcctgg gactgaacca ggggacagcg gggaacgtca gtgtacgtta
    13861 tcaggatggg atgctgatta cgcctacagg cattccatat gaaaaactga cggagtcgca
    13921 tattgtcttt attgatggca acggtaaaca tgaggaagga aagctcccct caagcgaatg
    13981 gcgtttccat atggcagcct atcaaagcag accggatgcc aacgcggttg ttcacaatca
    14041 tgccgttcat tgcacggcag tttccattct taaccgatcg atccccgcta ttcactacat
    14101 gattgcggcg gctggcggta attctattcc ttgcgcgcct tatgcgacct ttggaacacg
    14161 cgaactttct gaacatgttg cgctggctct caaaaatcgt aaggcaactt tgttacaaca
    14221 tcatgggctt atcgcttgtg aggtgaatct ggaaaaagcg ttatggctgg cgcatgaagt
    14281 tgaagtgctg gcgcaacttt acctgacgac cctggcgatt acggacccgg tgccagtgct
    14341 gagcgatgaa gagattgccg tagtgctgga gaaattcaaa acctatgggt tacggattga
    14401 agagtaattt cgtaaagcaa caaggagaag gatgatggct aacagaatga ttctgaacga
    14461 aacggcatgg tttggtcggg gtgctgttgg ggctttaacc gatgaggtga aacgccgtgg
    14521 ttatcagaag gcgctgatcg tcaccgataa aacgctggtg caatgcggcg tggtggcgaa
    14581 agtgaccgat aagatggatg ctgcagggct ggcatgggcg atttacgacg gcgtagtgcc
    14641 caacccaaca attactgtcg tcaaagaagg gctcggtgta ttccagaata gcggcgcgga
    14701 ttacctgatc gctattggtg gtggttctcc acaggatact tgtaaagcaa ttggcattat
    14761 cagcaacaac ccggagtttg ccgatgtgcg tagcctggaa gggctttccc cgaccaataa
    14821 acccagtgta ccgattctgg caatccccac cacagcaggt actgcggcag aagtgaccat
    14881 taactacgtg atcactgacg aagaaaaacg gcgcaagttt gtttgcgttg atccgcatga
    14941 tatcccgcag gtggcgttta ttgatgctga catgatggat ggtatgcctc cggcgctgaa
    15001 agccgcgacg ggtgttgatg cgctcactca tgctattgag gggtatatta cccgtggcgc
    15061 gtgggcacta accgatgcac tgcacattaa agcgattgaa atcattgctg gggcgctgcg
    15121 aggatcggtt gctggtgata aggatgccgg agaagaaatg gcgctcgggc agtatgttgc
    15181 gggtatgggc ttctcgaatg ttgggttagg gttggtgcat ggtatggcgc atccactggg
    15241 cgcgttttat aacactccac acggtgttgc gaacgccatc ctgttaccgc atgtcatgcg
    15301 ctataacgct gactttaccg gtgagaagta ccgcgatatc gcgcgcgtta tgggcgtgaa
    15361 agtggaaggt atgagcctgg aaggggcgcg taatgccgct gttgaagcgg tgtttgccct
    15421 caaccgtgat gtcggtattc cgccacattt gcgtgatgtt ggtgtacgca aggaagacat
    15481 ttcggcactg gctcaggcgg cactggatga tgtttgtacc ggtggcaacc cgcgtgaagc
    15541 aacgcttgag gatattgtag agctttacca taccgcctgg taaatgcgct gatgtgataa
    15601 tgccggatac gatttgcggc gtatccggct cgccgttacc gtaccaaccg caattgctgt
    15661 aaattgccgt cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc
    15721 atctctttat gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca
    15781 tatatccctt ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt
    15841 cccgcaacac ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac
    15901 tgctgcggtt gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg
    15961 aagtaatcac gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa
    16021 acaatcgttg cctgatgccc ggcctgtgtc actttgaccg ccagtgtggc ggctaagtca
    16081 tcggcttcgt tgccgctggc tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt
    16141 aatgcaggca tctcgtcgtg caactcttcc ggcattggcg gtcgatccgc tttgtaatct
    16201 ggtaaacgct gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg
    16261 tgggttggct ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag
    16321 ggcgaaccct gaacggcatg aatgcgacgg ataagattca gtgcatcgac aataagcaaa
    16381 tgaacagcca cgataacccc cttaaagatc aggcgataag ggtaacattc gatgccggaa
    16441 caggctatgg ctggttgaga aaacaggaag aggcttcgca aaacgaggcc tctgggagag
    16501 attaatcgca ggcaacgatc ttcatcgcca ggccgccgcg agaggtttcg cggtacttgg
    16561 cgttcatgtc tttacctgtt tcgtacatgg tttcgataac tttatcgagg cagacgcgcg
    16621 gttcgctggt acggcgcagt gccatacgcg cggcgttcac cgctttcact gctgcaatgg
    16681 cgttacgctc aatgcacggt acctgaacct gccccgcgac cgggtcacaa gtcagaccga
    16741 ggttgtgctc catcgcgatt tccgctgcga tgcaaacctg cgccgggctt gcgcctaaca
    16801 gttctgccag accagctgcc gccatagagc acgccacgcc aacttcaccc tggcaaccca
    16861 cttcggcacc agaaatcgac gcgttcatct tataaagaga accaatggcg ctggctacca
    16921 gcaggtaacg agccagtgag ttagcgttca cttcgcggat aaacttatcg tagtacgcca
    16981 gcacagccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg cgaccgccag
    17041 cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca accgccatcg
    17101 ggtcagtggt ggttttatcc aggctgacca gcatccggcg tagtgccgca gcacggcgtg
    17161 gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca ataccgccac
    17221 gcatgacttc ccagacgttc gccaggtgct gttccagctc ttctttactg tgcagcgcca
    17281 gctcgttttt catcatcagg ccagagagtg acagcccggt ttcctgacaa tgtttttgca
    17341 gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctatcc tgctggccaa
    17401 aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg ctgtaaacga
    17461 ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa aggttatctg
    17521 catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg tttgccagca
    17581 tcaggcgacc atgagtattc acatcctgaa taaaaccggg gatggaatcg atatccacgg
    17641 tatccggcag gttacccgcc aggcccataa taatggcgat atctgtgtgg tggcctttac
    17701 cagtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct ttaagcaggt
    17761 tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca acggtatgag
    17821 aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata ggaaatacat
    17881 cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag ctgaacagag
    17941 agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg ttgctgatgt
    18001 gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc atcaggaaca
    18061 ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg ctcgggttca
    18121 gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg ttcagcttgt
    18181 tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg ttgaagcctt
    18241 cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc gcgataatcg
    18301 gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac agaatcgaga
    18361 tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtacg cagctgaaga
    18421 cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc ttcgagcatt
    18481 tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa gagatgatcg
    18541 gagagtggtt gaacgagaac accattaccg gaattgccag ccacagggtc atccacagac
    18601 cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg ccgttccact
    18661 gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat accagaatac
    18721 tcatcgcttt aacgatcatc tgctcaccaa agcgaacgat ggtcatcata cccacgatca
    18781 ggatcagcga cagaatcgca cgcggcggtg gcgtcatacc cagctggtga gacatgaagc
    18841 tttcaacggt attggtgatt gccacgctat aaaccagcag gatcgggtag atagcgaaga
    18901 agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct acaacctcgg
    18961 tgatgtcttc gcccgggttt ttaccagaca gtacgaagcg agtcaggccg cggtgagcaa
    19021 aaaacgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata ccgccaacac
    19081 cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa aggcccagca
    19141 tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca atcgtgctgg
    19201 tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct tttagccagt
    19261 tcagccaggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga tggcttaatc
    19321 atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta tctatcagtg
    19381 atcttgatcc caaatacaat aatctgcttg gaagatagat tatcccaacg ctttactctg
    19441 taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga catctgactt
    19501 cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt tagttaatca
    19561 tggcgataat ttctttttca tagcgataaa atacggttta tgaaaatagg gaaacgtttg
    19621 tgttgcaaca taaattgtat gacatattaa ctgtgatgat atttgtcgcg tgagggtggt
    19681 aagaaacatt catataacaa atgaaatgag ttgatgagta agtgaccaaa aacgagaaaa
    19741 cccggcaa
//
LOCUS       DADLDT020000080        19652 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000080 DADLDT020000000
VERSION     DADLDT020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.080"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="JQH22_002701"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="JQH22_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFB2206966.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="JQH22_002702"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="JQH22_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFB2206967.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JQH22_002703"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JQH22_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFB2206968.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JQH22_002704"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JQH22_002704"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFB2206969.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="JQH22_002705"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="JQH22_002705"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFB2206970.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="JQH22_002706"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="JQH22_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="JQH22_002707"
     CDS             complement(5911..6954)
                     /locus_tag="JQH22_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFB2206971.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="JQH22_002708"
     CDS             complement(7200..7652)
                     /locus_tag="JQH22_002708"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2206972.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JQH22_002709"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JQH22_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFB2206973.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JQH22_002710"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JQH22_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFB2206974.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JQH22_002711"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JQH22_002711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFB2206975.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JQH22_002712"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JQH22_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFB2206976.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="JQH22_002713"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="JQH22_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12057..12578)
                     /locus_tag="JQH22_002714"
                     /pseudo
     CDS             complement(12057..12578)
                     /locus_tag="JQH22_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12962..13702
                     /locus_tag="JQH22_002715"
     CDS             12962..13702
                     /locus_tag="JQH22_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFB2206977.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13673..14440)
                     /locus_tag="JQH22_002716"
     CDS             complement(13673..14440)
                     /locus_tag="JQH22_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFB2206978.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="JQH22_002717"
     CDS             complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="JQH22_002717"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFB2206979.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15464..17908
                     /gene="fadE"
                     /locus_tag="JQH22_002718"
                     /pseudo
     CDS             15464..17908
                     /gene="fadE"
                     /locus_tag="JQH22_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(17951..18397)
                     /gene="ivy"
                     /locus_tag="JQH22_002719"
     CDS             complement(17951..18397)
                     /gene="ivy"
                     /locus_tag="JQH22_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFB2206980.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            18578..19348
                     /gene="yafV"
                     /locus_tag="JQH22_002720"
     CDS             18578..19348
                     /gene="yafV"
                     /locus_tag="JQH22_002720"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFB2206981.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            19458..19652
                     /locus_tag="JQH22_002721"
                     /pseudo
     CDS             19458..19652
                     /locus_tag="JQH22_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         4787 a         5052 c         5069 g         4744 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcattatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggga tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 aaaatcatct cttccgtcaa aatcctgcgc gcgcctaaaa aatggtcctg ccagaaaggt
    12121 tcggctaacc ggcttacccg aatggtttcc ccggtgcgca gtgactcaat aaactgaccg
    12181 tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaatagc
    12241 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta gtggtacatc
    12301 tcattggccg tgcgcgggag tttcgcctca aggatcttgt tgtaggcata aaaaactaac
    12361 ccactacaat cgaagccttg atcgggccgc gtgccgcccc agacgtaagg cttgccaagc
    12421 tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gcaggtgatc gctgcttaaa
    12481 aagtggttgt tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga
    12541 aaccattctg gatgctgttt tatctgctcc cggttgtggt ggcgctggcg agtaaaggga
    12601 gcaaaacgca taacgaaaac acgcctggat gcaaaaatct gccgagcaag aacgaagaca
    12661 tgaaagacat aaaccggctg aatgaaggtg agaattttaa aagaccggag gagtaaacgg
    12721 gattattaat tgcttatttg catattacta ttgagatttc ataatattag gaaataaatg
    12781 atacatgcgc ttttcggact attgaaaact gcccctgtgc taaatttgta aacccagcgc
    12841 cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc
    12901 cgatttggga tccattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag
    12961 tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg
    13021 tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat
    13081 gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tttacgtcaa
    13141 aatgggcgag caatatcaac tgctcgacag ttataaaatc tgtaaatatt ccggcggctt
    13201 agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg
    13261 taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat tccccaatgc
    13321 ttatgaccgt gcgcatggtt atgaagggaa atacctgatg atccacggcg attgtgtatc
    13381 catcggctgc tacgcaatga ccaatcaggg tattgatgag atcttccagt tcgttactgg
    13441 tgcgctggtg tttggtcagc cgagcgtgca ggtgagtatt tacccattcc gcatgaccga
    13501 cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc agctgaagcc
    13561 gggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg ccaatggccg
    13621 ttacgtggtc agcaagccgt taagccacga agtggtgcag ccacaactgg catcaaacta
    13681 cacgctcccc gaggcaaaat aagtgccatt cgcctggcat aattttttgc caggtttcat
    13741 tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc
    13801 tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg
    13861 tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtcc gaaagcagca
    13921 tgttgaaaac gcccttcgcc cgcagttcat ccgccagtga ggcgatatat ttaaacactg
    13981 ccgccatgtt gcccggcgtg cgcgggtaac gctgcgttaa tttatgcagc agccagcaaa
    14041 aggctttttc gctgtcggtt tcaccgaccg ggcggaagtt gccggtttcc agtgatttgt
    14101 agcccgtcag ttgtccgtta tgggcataag tccagttacg cccccataac tcgcgggtaa
    14161 atgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca
    14221 ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa ttaaagctgg
    14281 gttgtggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat
    14341 ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta aagcagatat
    14401 cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc cacaacagcg
    14461 ggagcgcatg gaccccggcg aagattgatt taagaatttt caggtcggat gcggcgtaaa
    14521 cgaattattc ggcctacggt tcgggtacag atttgtaggc atgataagac gcgtcagcat
    14581 cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa acgccttatc cggcctacgc
    14641 cagacttact taaccatctc tttttcaatt aactggatca ggatatggat cactttaatg
    14701 tgaatctcct gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc gatatccgcc
    14761 gtgccagcca ttttaccgcc gtctttaccg gtcagggtga tcactttcat ccccttctca
    14821 cgcgccgctg cgatcgcttt gatcacgttt gcagagttac cggaggtgga gatccccagc
    14881 agtacatcgc cttcgcgacc taccgcttca acgtagcggg agaaaatatc attgaaaccg
    14941 aaatcattac cgacgcagga aatatgacta acgtcagaaa tagcaatcgc cgggtagccc
    15001 ggacggtttt cacggtagcg accggtcaac tcttcggcaa agtgcatagc gtcgcaatgg
    15061 gaaccgccgt tgccgcagga aagcactttg ccaccggctt taaagctgtc tgctaacagg
    15121 accgccgcgc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt agccagcgtt
    15181 tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat atccttcagc
    15241 ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga agtacgggca
    15301 ggtgctatga ccaggacttt ttgacctgaa gtgcggataa aaacagcaac aatgtgagct
    15361 ttgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta caaccatatc
    15421 atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga ttttgagtat
    15481 tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct tatttatcag
    15541 cagtctgatt ttgctcgcct agacagccgc cctcggcgtt gctggtctgt ggtcggcgtg
    15601 ggtactggtg cctctgacca ttatcctcgt gccatttaac tttgcgccta tgcgtaagtc
    15661 gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga tgtcgcgcac
    15721 tgagaaagaa gcgattgatg cgggcaccac ctggtgggag ggcgacttgt tccagggcaa
    15781 gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg aagagcaagc
    15841 gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc agattactca
    15901 tgagctggcg gatctgccgc cggagttgtg ggcgtacctt aaagagcatc gcttcttcgc
    15961 gatgattatc aaaaaagagt acggcggact ggagttctca gcttatgccc agtctcgtgt
    16021 gctgcaaaaa ctctctggcg tgagcgggat cctggcgatt accgtcggcg tgccaaactc
    16081 attaggcccg ggcgaactgt tgcaacatta cggcactgac gagcagaaag atcactatct
    16141 gccgcgtctg gcgcgtggtc aggagatccc ctgctttgca ctgaccagcc cggaagcggg
    16201 ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat ggcagggcca
    16261 gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg caccgattgc
    16321 gaccgtgctt gggctggcgt ttaaactctc cgacccggaa aaattactcg gcggtgcaga
    16381 agatttaggc attacctgtg cgctgatccc aaccaccacg ccgggcgtgg aaattggtcg
    16441 tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta aagatgtctt
    16501 cgtgccgatc gattacatca tcggcgggcc gaaaatggcc gggcaaggct ggcggatgct
    16561 ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa ccggcggcgt
    16621 gaaatcggta gcgctggcaa cgggcgcgta ttctcacatt cgccgtcagt tcaaaatctc
    16681 tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga acgcttatgt
    16741 aatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt
    16801 gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc
    16861 gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc
    16921 ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat
    16981 gatgatcttc ggacaaggag cgattcgtta ccatccgtac gtgctggaag agatggaagc
    17041 ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata tcggtcacgt
    17101 cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac
    17161 gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga gcgccaacct
    17221 cgccctgctt tctgatgtct cgatggcact gctgggcggc agcctgaaac gtcgtgagcg
    17281 tatctcggca cgtctggggg atattttaag ccagctttac ctcgcctctg ccgttctgaa
    17341 gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact ggggcgtaca
    17401 ggatgcgctg tatcaggcag aacaggcaat ggacaattta ctgcaaaact tcccgaaccg
    17461 cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc
    17521 gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccactcgttc
    17581 acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg gtttactgga
    17641 agaggcgttg gtggatgtga ttgccgccga cccgattcat cagcggatct gtaaagagct
    17701 gggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc tgacgaaggg
    17761 gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc gtctgcgcag
    17821 tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga
    17881 gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt
    17941 tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg
    18001 ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc agccaggtga
    18061 gtttctcttg cgacgttttc tcatcaatag ccgagaacag ccccgtcatc tgattagatt
    18121 tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca
    18181 tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt
    18241 tcatcaccca tgcaggtagc ttatgccctt gcaccatctg attaaacgca gctttggtgg
    18301 tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga
    18361 tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc
    18421 ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat cagatatttc
    18481 caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta
    18541 aagttataaa acccttcgca taaaggagtt gtacaccgtg cctagtttga agattacgct
    18601 tttgcagcaa ccgttggtgt gggtggatgg tcctgccaac ctgcgccatt ttgatcgtca
    18661 actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt
    18721 tgccatggaa gcggcagctt cgtcgcttgc acaagatgac gtagtgaact ggatgacagc
    18781 taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa cggagtctgg
    18841 ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg
    18901 tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg cgcgagtgat
    18961 tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc tacgacttac gttttccagt
    19021 gtggtcgcgc aatctcaacg attatgacct cgccctgtac gtcgccaact ggccagctcc
    19081 gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt
    19141 ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg
    19201 ggtgattaat ccgcaaggcg agattatcgc taccgccgac gcgcataagg caacgcgtat
    19261 tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc cggcatggcg
    19321 ggatgcggat gagtttaggt tgtggtgagt gggggttgtg tttagggccg ggggctgggc
    19381 tagattacga aagcccctaa cttaaacaca atattacgcc agaagtcacc atttccaaaa
    19441 cacagcgtcg ctgtaccgtg ggttatctgc ccaaccgcag cgacacgccg gtgatcaaaa
    19501 tcagcgccaa atggctgaga aaagcgggat ttgagatcgg cacgggcgtt accgtgaaga
    19561 tctcggaggg atgtttgatc ctgcttggga taacaacgaa gtacaggaac tgcgcaggga
    19621 actttatcag gttaaacagc cagttaaggg ga
//
LOCUS       DADLDT020000081        19440 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000081 DADLDT020000000
VERSION     DADLDT020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19440
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.081"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(475..633)
                     /locus_tag="JQH22_002722"
     CDS             complement(475..633)
                     /locus_tag="JQH22_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFB2206982.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(913..1632)
                     /locus_tag="JQH22_002723"
     CDS             complement(913..1632)
                     /locus_tag="JQH22_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFB2206983.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(1629..2063)
                     /gene="psiB"
                     /locus_tag="JQH22_002724"
     CDS             complement(1629..2063)
                     /gene="psiB"
                     /locus_tag="JQH22_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFB2206984.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(2118..4076)
                     /locus_tag="JQH22_002725"
     CDS             complement(2118..4076)
                     /locus_tag="JQH22_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFB2206985.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(4140..4373)
                     /locus_tag="JQH22_002726"
     CDS             complement(4140..4373)
                     /locus_tag="JQH22_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFB2206986.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(4429..4908)
                     /gene="ssb"
                     /locus_tag="JQH22_002727"
     CDS             complement(4429..4908)
                     /gene="ssb"
                     /locus_tag="JQH22_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFB2206987.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSESWRDKQTGEMREQTEWHRV
                     VLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEILVKTTGTMQMLGSAP
                     QQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQPSEPAYDFDDDIPF"
     gene            complement(5058..6959)
                     /gene="ltrA"
                     /locus_tag="JQH22_002728"
     CDS             complement(5058..6959)
                     /gene="ltrA"
                     /locus_tag="JQH22_002728"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFB2206988.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVSSGDSAK"
     gene            complement(8439..9002)
                     /locus_tag="JQH22_002729"
     CDS             complement(8439..9002)
                     /locus_tag="JQH22_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFB2206989.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(9049..10410)
                     /locus_tag="JQH22_002730"
     CDS             complement(9049..10410)
                     /locus_tag="JQH22_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFB2206990.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10462..10692)
                     /locus_tag="JQH22_002731"
     CDS             complement(10462..10692)
                     /locus_tag="JQH22_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206991.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(11730..11921)
                     /locus_tag="JQH22_002732"
     CDS             complement(11730..11921)
                     /locus_tag="JQH22_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206992.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(11918..12340)
                     /locus_tag="JQH22_002733"
     CDS             complement(11918..12340)
                     /locus_tag="JQH22_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFB2206993.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12387..12812)
                     /locus_tag="JQH22_002734"
     CDS             complement(12387..12812)
                     /locus_tag="JQH22_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFB2206994.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13230..14006)
                     /locus_tag="JQH22_002735"
     CDS             complement(13230..14006)
                     /locus_tag="JQH22_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206995.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(14057..14491)
                     /locus_tag="JQH22_002736"
     CDS             complement(14057..14491)
                     /locus_tag="JQH22_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HFB2206996.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(14505..14726)
                     /locus_tag="JQH22_002737"
     CDS             complement(14505..14726)
                     /locus_tag="JQH22_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206997.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(14727..15410)
                     /locus_tag="JQH22_002738"
     CDS             complement(14727..15410)
                     /locus_tag="JQH22_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFB2206998.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15487..15780)
                     /locus_tag="JQH22_002739"
     CDS             complement(15487..15780)
                     /locus_tag="JQH22_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2206999.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            15927..16889
                     /gene="parM/stbA"
                     /locus_tag="JQH22_002740"
     CDS             15927..16889
                     /gene="parM/stbA"
                     /locus_tag="JQH22_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFB2207000.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            16892..17242
                     /locus_tag="JQH22_002741"
     CDS             16892..17242
                     /locus_tag="JQH22_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFB2207001.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(17365..17646)
                     /locus_tag="JQH22_002742"
     CDS             complement(17365..17646)
                     /locus_tag="JQH22_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207002.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(17774..18008)
                     /locus_tag="JQH22_002743"
                     /pseudo
     CDS             complement(17774..18008)
                     /locus_tag="JQH22_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(18944..>19440)
                     /locus_tag="JQH22_002744"
     CDS             complement(18944..>19440)
                     /locus_tag="JQH22_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207003.1"
                     /translation="QGLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSD
                     TKATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEAL
                     KQQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHR
                     LYYS"
BASE COUNT         4453 a         5391 c         4813 g         4783 t
ORIGIN      
        1 ccgttccggt cgctgcaaac ccgggttttt gcgtcaggga tggaagcccg ccagggcgga
       61 gacagcttcg ctggctccgt tcacgacagc ccgccccgcc agggggacgc ccggaaaagg
      121 cataagcagg agaacaacaa aacagacaat aatcagggca gcagtaatca gagtcagagc
      181 agtaatcaga gtcagagcag gaggaaaaag agaataatca aagggaaggt gatgtgattt
      241 ctggtaagtc ataaacagag aaggcggggg aaaagaacgg gcgccgcagg cgcacgttca
      301 tccccgttcc cggagggaaa cggccttcct ggcagtctgg ttgttcatgc ccgtacaatg
      361 ccagcaagga gaaaggggcc accggcgaac cagcagcccc tttataaagg cgcttcagtg
      421 cagtcagacc agcatcagcc ctgaaaaggc gggcctgcgc ccgcctccag gttgctactt
      481 accggattcg taagccatga aagccgccac ctccctgtgt ccgtctctgt aacgaatctc
      541 gcacagcgat tttcgtgtca gataagtgaa tatcaacagt gtgagacaca cgatcaacac
      601 acaccagaca agggaacttc gtggtagttt catggccttc ttctccttgc gcaaagcgcg
      661 gtaagaggct atcctgatgt ggactagaca tagggatgcc tcgtggtggt taatgaaaat
      721 taacttacta cggggctatc ttctttctgc cacacaacac ggtaacaaac caccttcacg
      781 tcatgaggca gaaagcctca agcgccgggc acatcatagc ccatatccct gcacgctgac
      841 cacactcact tttcctgaaa ataacccgct cattcaggct gtttacgggt aatctgtgtg
      901 attgttgccg catcacgccg cctcccggag tttgttcggc accatccacc gctccatctc
      961 acggacagcg tgaccggcct ctcttgctgc aagactggct tccgcaataa cctgccagaa
     1021 cggcatatcc tgccactgcc agcgttccat tccggccagt gacggaaaac gctcacccca
     1081 ctgccagaac agggtttcca ggtcatgctc tgccacccgg tcactccagc gtgcatacca
     1141 gcttccgact gtttccggcg aatggaatgc cagttcgatt tcagcctgag cgaccagcgc
     1201 ctggtacgca cgggctttct gctctgcctc cctcgcctgc tggcggctgt acttctgcat
     1261 gaccagcgcg gatttatggc gactgcgttc cctgacacga aaccgcaccg acgggaacaa
     1321 gcgttctgct gcatcgcctg gaagaggaag gaggcagacc tcgccctgtg tttcaatcag
     1381 cacatcgatg gcatacagcc actgaagacg gctgccgtgc agttcttctg cggtcaggga
     1441 cggcatgaaa aatcgcaggt cagacagcga tatcctgcgg cttccgttca gacagcggaa
     1501 aaacgccccc gcatacggat attccgccag cgtgtttccc tggtggcggc gtttttctgt
     1561 ctccgccacc gcccgccatg cggcctgttg ctctgcgctg aggggaacca gtgcctgtga
     1621 acgtgcgctc atgcctgacc tccttccgcc atcaggatat ggatgatgtc actgacgctg
     1681 tacccctgcg cgtccagtga cgccgccagt gccagtgaat ggctgacggc ttccggtgca
     1741 aaccgccgct gtacctgaac caccgcaaac ggctcaccac cggcattcac cagaaccagc
     1801 acccagacct gagagacatc gcccggtgaa cataacgcca ggtgaaaacg ttcgtgggca
     1861 ggcgtgaaac ggacctggac ggggaaaaat ccgccaaact cgctgccgta ctcgccgttc
     1921 atcgtccagt tgtccggcgc atccagttcc cgcatcacgg cgtgtgtcag ctcacgacgt
     1981 tcatcacttc cggcagcccg gatatcttca tattcctgtg cgctcatggt ctgtaataca
     2041 ttcagggtca gttcagtttt catcacggta tctcccttgc tgccgccccg tctccggcgg
     2101 ggcggtgtgg ttgttcatca ggcggcatca gccaggttat cggtgcgttc agtggtatct
     2161 gtctgtggat gtggtgcaca catccagcca ggcacccagc ggttgtcttt catatggtgc
     2221 tctgcatgtt cagccgcatc acctttcttc atcttctccg cgtcccgtgc ggcaccggac
     2281 agccctgcct cattcagggc tgcgataatc tgcggctttt tcaggtgtcc gaagaagttt
     2341 gcttttgtcg gctgccacca gtcgcgcatg tgaaagccga tggcgctttc cagcgtgtca
     2401 agcggactgc gtgacgtgtg accacactca cgggtctgga caccgttaag gctgcatgcg
     2461 gtgcagaaac tgagcaggga taacagcacc tcctgactga gtgacaggaa tgtcgtcatg
     2521 tcccgtgacc atccctccgg taacagggca gcaagacggg ctttttctgc catcagcgcc
     2581 atgaatgcgg caccttcctt ccccgatggc gcatcgctgg tcagcgaata atgtttacat
     2641 tccaggctga tttgtgctgg tttactgtac gctccgctgc caaacacatt caggcagagc
     2701 gtccatgcca gcagtgtcag ggatttgtcc ggttgctgca tcagtgctgc ctggactgcc
     2761 agcgtgcgtt cggaagacat tttcgtcagc agtggcagac tgatttcctc cactgacgct
     2821 ttctcctgca cctgttccgt acggtcagcg tcgtcagcca cgtcatcctc actgcgcaac
     2881 tgcacaccac gctggacaca cacgttgcca taacgccagg acaccaccac tccacctccg
     2941 gcacgcatct ccggcgtcca cgccctgacc ttcgccatgc agcgcatcag cttcatttct
     3001 gcttccagca ggtcagattc ctcacactgg ttttccagcg cgtcgtaacg cgtcatcagt
     3061 tcgttcagac gttcttcttc cgcctccgtc agcaccgctt ccggctccgg cagacagcgg
     3121 tatgttccgg catcctcacg gcactcaccg acaggctcca tgcgtccggc gcaccattcc
     3181 cagccttcgg cttcccgaag gaactctgcg acagcctgga gtttttccag cagggcggca
     3241 tcgagcgcca cgcagtccac atagccacct tcgtcgtcgc tgaacaaatc ggtgcgcagt
     3301 tcgtctggcg agaaggcatc agcccccacg aagcggaatt tactgttccc cgccaccgcc
     3361 acttcacttt cggtcaccag acgacgaatg gtctgtactt ccggtttacc gccccatccc
     3421 gactgacagg cggcttcaaa cacctgcacc tgacgcgcgg tgtcgttctc cagcgccagc
     3481 gcctgacagt gttcggtggt gatgcggtct tctgccagcg catcgaggat gacaggcgca
     3541 aggtcagcca gtttcagcat tcgctgaacg tggcggggtg aatagcccag caaatcaccg
     3601 atttgtgcag gtgttttgcc ttcctgcgcc attgcgcgga atccggcaat ctgttcggca
     3661 gggtgcatat cccgacgctg accgttttca gtcattgatg cagccgtcgc cagctcctgc
     3721 ggaataacct tcacgcgtac aggccagtca gccggaagga tgccacgctc tgccagcatg
     3781 ttgagtgctg ccagtcggcg accacctgcg gcgacaccgt ggcggtcacc aggcagggca
     3841 tgaacaacca gattctgcaa caggccaacg cccttaatgg actccgccag ttcgctgacg
     3901 gactctgcgg aatacggcac cgtgcgcaca ttcagcggtg atttaatcag cgaggccagc
     3961 gacacgctca cttcctccgt ctgctccagc agggcagaca ggactgtttc ctgcgttttt
     4021 gcaggtttac ggctggattt acgctccgtt tttgtcttag actctgtaac tgacatatat
     4081 ttctcctttg accgggatgg ttgtatgtct gccggacgcg ttcccgtccg gcacctctct
     4141 tacttaagaa tgccgaaatt ccggatgaca tggttcatcc agtacccggc accgtcctca
     4201 aaattccagg tacgccagac caggccactg ttattgcgga caaccaggcg aaaatgcgtt
     4261 ccctgatcat cctcgataaa gacattccgg tactgtgcgg caacggcttc agcctgttcg
     4321 cgggtaaagg agccgtcagg cagcccctga aatattctga aatattcgct catcctcata
     4381 tctccggtga tgccccgcac tggacggggc ggttgtcact gtctttcgtt agaacggaat
     4441 gtcgtcatcg aagtcatacg cgggttcaga cggctgctga ggctccggct gcgcggcctt
     4501 acgtccacgg ccttttgttt tcgcgctccc ttttttcgtc gtgtcagcac tctgtggctg
     4561 accattctgc tgaggctgcg gttgcgcctg agcgttctgc tgtggtgcac tccccagcat
     4621 ctgcatggtg cccgtggttt tcacaaggat ttcagtgacg taacgggtaa tacctttatc
     4681 ttcccagcta cgggttcgga gttgcccctc gatgtagacc tgcgcgccct tgcgcagata
     4741 ttcacctgcc acttccgcga gcttgccgaa cagcaccacg cgatgccatt ctgtctgctc
     4801 ccgcatctcc cccgtctgtt tgtcacgcca gctttctgac gtggccacct gcaggtttgc
     4861 cactgcgccc ccgttgggga tgtaacggac ttccggatct tttcccagac ggtagggtag
     4921 ggtacgtgtg gtctttcaag ataaatggcg aatatgtcct gcttatcttg agttagatca
     4981 ccgttacccc ccctccgaac cgtacgtgca cctttcaatg catacggctc tccattgcta
     5041 gcttattggg gataaactca tttagcagaa tccccacttg agacaggtgg ttgcaggagt
     5101 attcttccct tttcctgtct cagatcgggt agttttccgc tatgtaactt gtcgtggcaa
     5161 cttgcgcaca aaatcatcgt tttccggcgg cgtgcgatca tcattagttg ccacaggctg
     5221 ggtttatatc cggccttacg acctttcttc aggtctttta gctttctgac atgatgtact
     5281 tcgcagcgcc cagtcttcgc acaatactcg cagatccttg cccctaatcg acgcatgatc
     5341 tctgttgtgc gggttgtgat atgtgcggtt ctcggttccg tatcgacaat cgttgttcgg
     5401 ttgcgattga catgtttgag tttgaaaacc tcaatctttc gggatccatt tttggcctga
     5461 acggtaatag ccagatctcc attattttgc cgtagcgatt tggcgacttt attcactgaa
     5521 ctacgtcgtt tattcgctag ggtcttaaaa aggcttattt gccatatgaa atataatttt
     5581 gataatttat atttcatgtc cagcgccaga ctgtagtagt tcgccaatcc gcgcatttcc
     5641 gcgttgaaag tcagaacaat ttcagcttca ctgagatttt gtagacttga acgatgtaca
     5701 gccttcacat tctcataaca gccatatttc ttttggtgac aaaatttagc ggctatattg
     5761 tcaggaacga agagctgcac aattcctgtg gtggatctcc ggagagcgtg ccgcccttgc
     5821 attttctgct tcagcaattt ctttgcatct gcctgtctga tttcatagcc taaaaaatta
     5881 actccttctg agatatggcg gatcctggtc tttgcttcat taacttccaa tcccagtgaa
     5941 gtagtaataa agtttctgac ctgtcttgat atatgttcag catctttctt cgagccgatg
     6001 acaccaatta cgaagtcatc agcatatcga acatagtaca tacgcctgaa gtttggatcg
     6061 ttcatgacct gagatggtat agaacgtttt tcttcaagca gtcgttcaat ttctttcttt
     6121 atggcttctt tttctgattc agaatcagtt gccataccgt ctactttctt tcgtagttta
     6181 tgaatcttat gggccagcct gttcatatct ggattcgctc ttcgttttgt accgcttgta
     6241 tatgcaggaa ttaagttctg ttctacaaaa cggtccagtt tgtcgaggta tatatttgcc
     6301 agtaagggag aaattatccc accttgtggc gtgccagaat atgagcgatg gtatttccaa
     6361 ttgtccacat atcctgcctt cagccactgt tcgattaatt tcaggaaatt cttatcctta
     6421 attttctctg ccagtgtatt cagcaatagt ttgtggggga tattgtcaaa gaaacctttg
     6481 atatcaacat ccacgatcca tttaactccg ttccagactg gacgaataga tctgagcgca
     6541 gtttcacacg aacgttttgg tctgaatcca tgagagtgtt cagagaatac tggttcataa
     6601 accctttcca gaataattct tgctacttct tgcgccagtt tatcgtctcc acctggaatc
     6661 cccagcggtc tggtttttcc gtcctttttt gggatctgaa ctctgcgtac aggtttcgct
     6721 acgtattctc tactccgcaa tgtttttgcc agcttctctg ctctttcgat agagaagcca
     6781 tcaatggtat ctgttgatac accttgagtc agtgcacctg cattggcccg tatattcacg
     6841 taggcttgca gccacagtcc gggctcacac gcaattttgt atagattacg tactggatac
     6901 cctttttttg aagcatcctc aattccatct aatcttttct caagttcatc aggcagcatt
     6961 ggctaaccct cctcttagag attgatgtat ctaacaactg tctcccttcc ccgcagcact
     7021 gtgaggcttt catctccctt ccggttgatt tatacctttg ctgcttccgt ataatgcgga
     7081 ctgccgtagc ccattacagg ccgaccttga gtactatgga gactccgcta ccatgctggg
     7141 tataaatcca gtttaggtag tcccgtagtt tcacggtgtg gagatgcgat gtacttagga
     7201 tgtctttcga tcgtttaact atttcatcaa tagctggccc gttagagtgg tcacaatcaa
     7261 cgtagggcgg atttcttgtg ctattacggg aataacgctt caatcgttct gcgttattgg
     7321 tttcctgttt cccacataga tctcgacttc aacaaaccca gtttatccat atacatcttg
     7381 cattgagtgg tgatacttat aacgatttcc gtattcacca cccttttctg tggatgctat
     7441 gttcacctct ccttttcagg atctccgagc ctgttaccaa gcgaggctaa ccacagtatg
     7501 caatggctat accactaagc catgttgctt ttcatgcaac actccataaa acaccgcgct
     7561 tcaacggcgc acaccgacaa gaatgacctt gttaatgcca cgaactgcca tgatgtgtct
     7621 ccttctgttg atgttttcag cccccatcat ctgttgagtt tcgggagccg cggttttgac
     7681 gttttgcggg gacctgctct ttgttccctc cggtattcac catcacctgt ttgagaacct
     7741 tcgccgcatg gggcatcact gccacggcct gacgcacctg tcctgggcag aacgcaaaat
     7801 tttttaccgg gttttgggta aaaaattttg tgggctgttc atttaccctg gacaggtgcg
     7861 gcaggttgtg gctgtctatg cccccggcga caggggctta aacaggtgaa tggcaccgga
     7921 cagaacaacg ggcagggacc cgccgcaacg acgtgaagcg cggtttgcga aacggcgttg
     7981 ctccttacat cccggcgtct tttgccggga tgccgggagc gcaggccacg gcgcgacaag
     8041 ggtggcgcga ccgttccggt cgctgcaaac ccgggttttt gcgtcaggga tggaagcccg
     8101 ccagggcgga gacagctccg ctggctccgt tcacgacagc ccgccccgcc agggggacgc
     8161 ccggaaaagg cataagcagg agaacaacaa aacagacagt aatcagagca gcagaaaaaa
     8221 gaagaacagt agcagtagca gtagcagtag cagtagcagg aacaacaaca tcatcagtaa
     8281 caggcaaatg acaggtctga aaagacagaa gcccacagca gaacgtccgc gtaatgaaag
     8341 gggcggcgcg taagcgcggc ccctttcagc gccaccggcg cgcggtcttt tcccggcgcg
     8401 ggcagacggg catttatcct cccgcctgcc cggattcatc aggcacgcag acgaataatc
     8461 atcgtcggaa cacgggtata agcaaatgtg ccgcacggca gcacctcgcg gacatcagaa
     8521 aacggtaaca gcttctcctg ctggcgcggc ccgttcagac agacggcaac cagaacgccc
     8581 cccggacgca acagggaaaa agcccgcagg atatgccgga tatcctgccc gtgactgaac
     8641 ggcggattca taatgatccg gctgtaatac tgcaccggct gccattccat gaagtcgcca
     8701 cactgtaccc tgacaccgtt aaagttttcc cgcagatacc ggaccagccc gctgttaatc
     8761 tccaccgcat cacacatggc ctccggcgcg gtatcccgaa tcgcccgcag tatggccccg
     8821 gtgccggcag agggttccag aatgtgatca cggttgctga tttccgccag cgtcaccagg
     8881 cgatcacaga ccggggcagg agtcacaaaa agctgagatt cagcccggat ttcagcaacc
     8941 ggcgcggcgt tatccagcct tgcgcgcatc agccggatac gttctcttaa ctgactgtgc
     9001 atgtcattat ctccgtcatc ccccggggat ttcccggggg gatatacctt atttctgcgg
     9061 gatttccgtg attttcatgt cggtgatata cacattcacc agacggaaat tattgtccat
     9121 tgtgcggcgg tagcggtatg ccccgtggtc ttctgcttct tccacactgc gcacggcctt
     9181 acagtcccgg ggcagggcgg cccactgcgc ctttgtcatt tcccggaaac cttcccccgg
     9241 atagttgact accggcggac gcttcgcggc ctgactggcg acggcagcct cttctgccga
     9301 tggtgctttg tagtccgtga tgcgatcggg cgtcactttc atcgtgccgc tgtacccgag
     9361 aaaactgtaa tttggcgttg tgactgaact caccgccccg ttgcttttgt tcacgcggat
     9421 gatagtcagc cactcgcccc ggctgaaaac ctgtccgccc ggctcaaaat cctgtgtccg
     9481 ggtaaccacg ccgccgcttt cgtccagcat ggcacgctca tagatcaggc ggttctgata
     9541 gtggttaacc cagcgttgct gatgctgaat ctgccgttca tgacagcgga tcgcaatttc
     9601 gcgggcctgc tccgtggtga tgatgtcatc atccagggcc gaccataacg accggctccc
     9661 ttcatactgg cttttttctg ccgggcgcgg atacgtatcc agcgggaaac aggcgctgat
     9721 atggtcatga ctgctgatga gttttgccat attcagatcc agcgattcag cccgccacat
     9781 cgtcagatat ttctgcgact gtgcgatggt cttttcagcc ttgcgcaaat ccgcttcgat
     9841 ttttttgata cgacgccagc gaacgtccgg gcgttcttta tacttcgcgt gaagcagtgc
     9901 cgaccgcgcc cgctcctccc agtattccgc acgttcaaag agcatcacgg cacgtttcat
     9961 gccgttttca atacgctgcg catcacgacg ggcgcggcgt tcgctgtgat gccccacaag
    10021 aatgggctga ccgggcggga tcatcgcggc cagtctttcc acttcatcaa gtgcctgtgc
    10081 ggattcactg gcccgctttc cgctgtatcc ggtaaaccgc tccgcccgtg cttcctgacg
    10141 ttcagcgagc gtgctgtctt catcctcaat ctctccggca agtgagagga gcacgtcttc
    10201 ccggcccggt gtccaggccg gcgcgacaaa cagcgcctgt ttgggtgccc agcgaaaacc
    10261 ggcatcatgc acttttttat acgtctccgg gtcaagacgt gacgcggcat acaggcgcag
    10321 tttgttgtca tccggtgagt aggtcgcgcg gtacatctgc acagggctgg cggcttccgg
    10381 ggcggtcatg gtgattgctg ctaatgtcat aaaatgttct ccttccgtct gttgccgccc
    10441 ctcgcggggc ggctcacctc atcagttaaa agcgcgtttc agttcgagtg tgcggagcac
    10501 ctgcccgaaa gccgtgtttt tcatggcggg gatgtgtaca ccacgccccc ggtgaaaact
    10561 ccgggctatg tgtcgcccga aggctttcaa cacggggtca atttccggcg tggggatgtc
    10621 ttcgcgtctg aaacggggat gacggcgggg ctttacagcc gctgcggaag gtgaaacaat
    10681 gcttacagtc ataaatggat tccttatgac aaattggtta acaatgccgt caaaattggt
    10741 cgcacaattc ttacggcgtg gattaagcag gtgcctgacc tgcttttttc atatctgcat
    10801 ctgacgcttt ccggtgtttt cctccttctg ttcctgcgtt ctcattacct gtcagccgga
    10861 tgactgacag aacaaataaa tgcgtattgc cggagcctga aataaatcct gatttttaaa
    10921 gaacatcacc cgttgagttt cgggagccgc ggttttgacg tttcgcggga acctgctgct
    10981 gtattcactg tggtatgaac ttcacccgtt tgagaacctt cgccgcaagg ggcatcactg
    11041 ccacggcctg acgcacctgt cctgggcaga acgcaaaatt ttttaccggg ttttgggtaa
    11101 aaaattttgt gggctgttca tttaccctgg acagtcagtg cggcaggttg tggctgtatc
    11161 agcccccggc gacagggact catacgggtg tgtggcaccc ggcaggcaat gagcaggggc
    11221 ccgccgcaac gacggagagc gcgtgcgcga aacggcgttg ccccttacat cccggcggcc
    11281 ttttgccggg atgccgggag cgcaggccac ggcgcgacag aggcggcgcg accggaacgg
    11341 tcgctgcaag ccaggggttt tcacgcatcg gaccgtagcg gcagcccgaa accgcgccag
    11401 cgggttcggc ggagactggc aggtgcgcca gcagctgcct gtcggagtcg ggcctgacga
    11461 ccggatgccg ccaggcagcc gggagccgaa ggtgcgcccg gcagacagaa caacactgca
    11521 gtagtcagaa tcagagcagt agataaagga acaacaacat caggaacagt tccataacat
    11581 aagaaagaaa cactcctgag tcagacacat cacagacggg aagggtaacg ggcgacgcgc
    11641 cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc aggggttttc gcgcgggctg
    11701 acaggcggcc tccaccgcct gcccctgtat cacctgttta tgctgccctg aaggatgtag
    11761 tgaccgccga ggaatacagc ctgctgattc gccacaaaat cgcagatttc atcaacaatc
    11821 tgcgcagtgg tgagcggttt ctgccccatt tcacgacgcc gcgcattaat cacggctttg
    11881 taattgcgca gaatttcgcg ggtttctgtg ctgatattca tgacatgtgc tccccgtcca
    11941 gcgcctgtaa cacaacgtcc attgcttcgc gttcttccct gacgaatgcg tcaccgtcgc
    12001 ccccgttctc gctcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccatca
    12061 ctttttccag catcaccgcc ggaactgtca cctgacggct ggcgcgcttt tcttccatca
    12121 gctcacgaac aacgtcgtga acaatgctga catccgcacc atgcgcaaag gcatcgtcca
    12181 gtcgctgcat gacggtttcc agctcatcgt ctgtcagact ccagtcctgg gctatgtgac
    12241 gcacatcccc acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtgt
    12301 tcagcacttc gcggataatt tctttaacag tacagtacat gttctcgtct cctttacagg
    12361 ggcggcattg ccgccccgtc cgttaatcag tccagtgccg cgtaaatcgc gttgcattcg
    12421 gggtgaaatt cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt
    12481 cctgcatccc cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaagtcaga
    12541 atgatgcctg cggcatcagc actcacggta cggtcaaacc agtttgcacc gttcaccatg
    12601 tggaaacggt cgccatccgg catcatgtaa ccgccaccgt ccggcaggcg gatgaagtgc
    12661 cagaagcctc cctgatattc cggcatcatt ttttgcgcca gcgcgtacac atacgcctcg
    12721 gcatacagaa aatcgttgcc aaacagatac ggtagaaagg agagacggtc gcactcttca
    12781 acgttcagag tgacaggttt cgcatattgc atgatgtttt cctccgttgt tcacatattc
    12841 cgcgacaccg ggcggtgccg tgcctccata aaagccgggc gataaggcgg ggatgcacag
    12901 ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg gagttggagg gtgagaccgc
    12961 gaaatctgag cgggtgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga
    13021 ccgcagcggc gaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca
    13081 cagcccgcac acgacgctga ggccacataa ctgaaaaacc acaaggccat gccctgcatg
    13141 gccgggtaaa gcggagccgg cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa
    13201 aaaggcgggg atttaccccg ccaacgtcat catttccgga tttttgccag ggcatccgcc
    13261 atgctgcgga tttcggcccg ggcttcccgg accacctccc gcagaacacc acggtcaaac
    13321 cagcggcggg gcggttcatc cggtatacgc agcacatcgc aggccagccc actggacagg
    13381 atatgctcaa tacaatcgct gaccaggtac atttccaccg acgaaaaacg tgctccccgg
    13441 ttcacgatgg catcgagcac cacgggcagg ttttcgtcat cttcatcgag ccagcagggg
    13501 tgatcgcttt ccttcacaac cagacgaaag cggcgcgtct ggcgagtcag caacgtctga
    13561 ccggtggtgg tcagcgccgc ccggatacgc cgcgcatctt ctgccacccg gcaactgaca
    13621 ctgcgtaccg catcgcgcac cgccgcaagt acagccctga tttcgtcttc ggtcagccat
    13681 tctccggcat acgggaaaga cacctgcgtt ccgtctgccg gaagtaccgg cagcgactgc
    13741 cactgactgc cggacagtac cagttccgcc ccggttccgg tcagtttctg aatggcggag
    13801 gccaggagcg gcggcagtac gccgccgtga cggttgataa gtaacgtcgc cagccggggc
    13861 gcatccagca gaacataaat gctgatccag cccggatact tcgggttacg ctggtcaaca
    13921 tccgtctctt ccggccagcc ctgcgccagc agcaggttgc gggcgtgacg acaaatcagt
    13981 gcgttcagcg tgctgttttc gttcatgatt aagcgttctc cctgtatcgc cgcccgcagg
    14041 gcaggcggcg ataacatcag tcgttttcgt gtgtgttgtt tttcagcagg gtgcgggcct
    14101 gattaaccac ctcctgacgg cgggtgacgc attccgggac ggcgaggcca tgatcccggg
    14161 cggcccattc acgtttccac aatgcctgtt cagcggtctg aatcagggac gccagcagtt
    14221 cagcagggac ggtcaccagg cggttttcgc tctcgttgcg gataatctcc atcaccgcag
    14281 cggaagaaat accggactca atccgctggt cttccgggat gtcctccagg cgtgccagga
    14341 cggtcctgat ttcatgaccg gtcagagaaa tttccatacc gtcagccagt gcctgaatct
    14401 cttccgggga ccagataacc agcatcagcg gcatgtctcc cggatacttt actgccagct
    14461 cccggcatag cgtttcgcat gttccgtaca ttctcgtctc cttctcaatc tttaaagcac
    14521 gcttccagtt ccagaatttc ggtatatgcc tccatcagga ggcggtttgc gcgaaacagc
    14581 gtctgacgca ccagacgttc cggcagggtg tcggcattaa agctgtaccg ctgctccatc
    14641 tcattcatgg ttgtacgcag gtcgcacatg gcattggcca cctccgccac ttcggcgcgc
    14701 agtttttcgt gtcctgcata gttcatttac gcagcctccg gcataaacca gtcatcattc
    14761 gcggccccct gctgcatggc ccgttgcacg gcggcaaggc gttgctgccc ggcacggtga
    14821 tactgctcaa gcagctcgat accgatatac cggcgtccgg actggagggc ggcgacgcag
    14881 gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt tcgggtgtgt gaagctctca
    14941 atcagcggtt gcaggctggt aacgggcttt tccgtcgggt gatggcgatt gcccgaatat
    15001 ttccagccca gcacgtccgg cagcgggttt tgtggcagac gtggacggcc ttttgccagg
    15061 atgtaggcac attcgtggcg atagcccaca tatgcggcct tcgatgtgta gtttttggtg
    15121 aagaccaggt gaccaacaac gctgaatccc gcatttttcc aggcggccat aaagcgatcg
    15181 acgcggttcc agccatagaa gctcaccatt aacgcgtctt ttttgagtac gcggtacatt
    15241 tcattacagg ctggttgcag ccattcatcg gttttatcgc cggcgatggt gcgcccggaa
    15301 cggtcacgga aaccgacgag atacggcggg tcggtgagga tgaaatcgac agcattgccc
    15361 ggaaacgtgg ccataacacg cacgcagtca cccaggataa aacgggacat aacagcactc
    15421 cttctcttac agagggcacc gggagggcac ccctgtggtg cccgttcccc gctgcctgtg
    15481 ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg gaataccgca gccgtcgtct
    15541 ttttgacggc gaggatatgc cgcgaaagcg gcttgaaggg cgttcatcag aaaagggcga
    15601 cgggctggcg tacactgtca ccaacgccag ccgggagcgg taaaagcgcc cgtcccgtgc
    15661 ccggccggac gcgggacact gaccacaggc agcgttcctg ccgcgccgcg tcagccggcg
    15721 cagtaaaaag aggagcccgg cgacatcttt tgttacccgc caaacaaaac ccaaaaacaa
    15781 cccataccca acccaataaa acaccaaaac aagacaaata atcattgatt gatggttgaa
    15841 atggggtaaa cttgacaaac aaacccactt aaaacccaaa acatacccaa acacacacca
    15901 aaaaaacacc ataaggagtt ttataaatgt tggtattcat tgatgacggt tcaacaaaca
    15961 tcaaactaca gtggcaggaa agcgacggaa caattaaaca gcacattagc ccgaacagct
    16021 tcaaacgcga gtgggcagtc tcttttggtg ataaaaaggt ctttaactac acactgaacg
    16081 gcgaacagta ttcatttgat ccaatcagcc cggatgctgt agtcacaacc aatatcgcat
    16141 ggcaatacag cgacgttaat gtcgttgcag tgcatcacgc cttactgacc agtggtctgc
    16201 cggtaagcga agtggatatt gtttgcacac ttcctctgac agagtattac gacagaaata
    16261 accaacccaa tacggaaaat attgagcgta agaaagcaaa cttccggaaa aaaattacat
    16321 taaatggcgg ggatacattc acaataaaag atgtaaaagt catgcctgaa tctataccgg
    16381 caggttatga agttctacaa gaactggatg agttagattc tttattaatt atagatctcg
    16441 ggggcaccac attagatatt tctcaggtaa tggggaaatt atcggggatc agtaaaatat
    16501 acggagactc atctcttggt gtctctctgg ttacatctgc agtaaaagat gccctttctc
    16561 ttgcgagaac aaaaggaagt agctatcttg ctgacgatat aatcattcac agaaaagata
    16621 ataactatct gaagcaacga attaatgatg agaacaaaat atcaatagtc accgaagcaa
    16681 tgaatgaagc acttcgtaaa cttgagcaac gtgtattaaa tacgctcaat gaattttctg
    16741 gttatactca tgttatggtt ataggcggtg gcgcagaatt aatatgcgat gcagtaaaaa
    16801 aacacacaca gattcgtgat gaacgttttt tcaaaaccaa taactctcaa tatgatttag
    16861 ttaacggtat gtatctcata ggtaattaat gatggacaag cgcagaacca ttgccttcaa
    16921 actaaatcca gatgtaaatc aaacagataa aattgtttgt gatacactgg acagtatccc
    16981 gcaaggggaa cgaagccgcc ttaaccgggc cgcactgacg gcaggtctgg ccttatacag
    17041 acaagatccc cggacccctt tccttttatg tgagctgctg acgaaagaaa ccacattttc
    17101 agatatcgtg aatatattga gatcgctatt tccaaaagag atggccgatt ttaattcttc
    17161 aatagtcact caatcctctt cacaacaaga gcaaaaaagt gatgaagaga ccaaaaaaaa
    17221 tgcgatgaag ctaataaatt aattcaatta ttattgagtt ccctttatcc actatcaggc
    17281 tggataaagg gaactcaatc aagttatttt cttaccagtc attacataat cgttattatg
    17341 aaataatcgt ttgcactgtc tctgttattc aggcaatttc aataaaggca cttgctcacg
    17401 ctctgtcatt ttctgaaact cttcatgctg catttcgatt tttcttctaa aatcataact
    17461 tgcatacctg ttctttttat gtatatccag atctattttt gtgacgagtt caaaccacaa
    17521 tgatagcact ccccatgcag cgttatgaaa catttccgca acagatttag agtctgtatt
    17581 aaccgcttca gtcatgtagt cgctaatacg ttctgcagtg attttcgtta tttcatcgta
    17641 attcatttta tcccctacaa gactatttaa tggaatgaaa aatatttggt ctgataacaa
    17701 tgctcctgaa ttaacacctg caggctatgt taaagttgcc ctcacacaca atctacctgt
    17761 tttctcgtgt gtgtcacagg cgcaatacat aaatagtctg gccattaagt tcgtaacgga
    17821 tttcgtactc gccagcaaaa atccgtctga catccctggt ttcaaactga aacaactgtt
    17881 catcccatac gtggatgagt taacagaatg accggcgcct gtgtcaggga ctacaccgtt
    17941 ttggctgcag ctggtttact gaccaacgcc agaaaatcat ataactgcgt caatgtcagc
    18001 gtatccagcg cgactgtatt atttttctct gcaaccagaa tattgcatta ggttattacc
    18061 aggtgttcta atgtacaggt aaagacgttc tttataatct gaaatctcta cttttaatcc
    18121 ctcagacttt gcctgctgaa agccctcgtc aaggtgcgct tttattaggt taatgtctgg
    18181 atgtcttgta tcgggggcaa ttctgtattg gtgtaacgat ggggtggatg ttccctgcaa
    18241 taagacctcc agatagagat tggcacaacc attcccatta tttctaatag tttattggta
    18301 atcacaaact gtatatttag ccattatatt gaatcctgtg aagtaaacgg cgacggctca
    18361 aaattttatc aaacctaaga taattctacc atcaaagtta ataatttgaa tttatatgac
    18421 ggctctatgg gggggtaaaa atccccgtgc agagaggcga ttttcagagt tgaaaacgtc
    18481 gtcgttaaga acaccaaagt cagtaccacc aacccggaaa gtagcttcat gcaccgggac
    18541 aacagcagtt ttggtgtccg aaatcgtaca attctgtaca tctacgcaag gaaatgtacg
    18601 gaagtaagcc aaatggttat cggacggtcg gaacatttcg tacagccttt acgttcagtg
    18661 agttagctgg cctagatgta accactcgtt caactcaatt ctcttagcgt gggaaaattt
    18721 ccgttttcca agcggccccc tcaagcggac cccttatagt acaataattt tcgatatcca
    18781 aactgccccc aattacatct ccagtctctg gcagcagaga tcatatattt ccattgccgc
    18841 cgtagccacg atattctggc tattcacaaa aacaatttag ccccaaagcg cagtaaaaaa
    18901 gtacactatg aaggtgtatg ataccttcat agtgcttaag taattatgag taatataatc
    18961 tgtgtaaact actaattgct tcaccgtcag tgattaaatg cgtagctgtt ccttgtctta
    19021 tataatacct attttcaaaa tgacagactt ctccgacact ttttatttcg atttttaaaa
    19081 catgcagtcc ttcatatgaa cataatgaaa tatcttttag tatttgttgt ttcaaagcct
    19141 cattaaaatt aaatgattct attttttgtt ttatgaacag aaagtaatta tctatgtttt
    19201 ttgattgaat tacagcttca tgatcaattc cattgatata atagccattg cactcaatag
    19261 gggtaacacc atataaagtc ttaactctgt ctgccgtagc tttggtatcg gaaacaccaa
    19321 ctaaaatata accattagat tctctaccga tgttatttat ggcgacagcc gtttggacta
    19381 tattaatgaa acattcttcg tctattttat tctcgcctga gagaataaat aatccctgtt
//
LOCUS       DADLDT020000082        19375 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000082 DADLDT020000000
VERSION     DADLDT020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.082"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="JQH22_002745"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="JQH22_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFB2207004.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="JQH22_002746"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="JQH22_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFB2207005.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQH22_002747"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQH22_002747"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFB2207006.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="JQH22_002748"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="JQH22_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFB2207007.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="JQH22_002749"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="JQH22_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFB2207008.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="JQH22_002750"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="JQH22_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFB2207009.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="JQH22_002751"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="JQH22_002751"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFB2207010.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="JQH22_002752"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="JQH22_002752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFB2207011.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="JQH22_002753"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="JQH22_002753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFB2207012.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="JQH22_002754"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="JQH22_002754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFB2207013.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="JQH22_002755"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="JQH22_002755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFB2207014.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="JQH22_002756"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="JQH22_002756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFB2207015.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="JQH22_002757"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="JQH22_002757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFB2207016.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="JQH22_002758"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="JQH22_002758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFB2207017.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="JQH22_002759"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="JQH22_002759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFB2207018.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="JQH22_002760"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="JQH22_002760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFB2207019.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="JQH22_002761"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="JQH22_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFB2207020.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="JQH22_002762"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="JQH22_002762"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFB2207021.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DADLDT020000083        19357 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000083 DADLDT020000000
VERSION     DADLDT020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19357
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.083"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..2145
                     /gene="pbpC"
                     /locus_tag="JQH22_002763"
     CDS             <1..2145
                     /gene="pbpC"
                     /locus_tag="JQH22_002763"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFB2207022.1"
                     /translation="GLFIPRRGHNMLDNLIGARYLEALINYEDRWFWKHPGVNPFSVA
                     RAAWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILI
                     LYLNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAE
                     AARNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVT
                     TLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVD
                     MVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMS
                     EALVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDM
                     AAAYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVA
                     PLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNIL
                     LSRSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQ
                     EGINGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTI
                     CPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERG
                     RNVTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            2295..2726
                     /gene="ndk"
                     /locus_tag="JQH22_002764"
     CDS             2295..2726
                     /gene="ndk"
                     /locus_tag="JQH22_002764"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFB2207023.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2876..4030
                     /gene="trmG/rlmN"
                     /locus_tag="JQH22_002765"
     CDS             2876..4030
                     /gene="trmG/rlmN"
                     /locus_tag="JQH22_002765"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFB2207024.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4315..5328
                     /gene="rodZ"
                     /locus_tag="JQH22_002766"
     CDS             4315..5328
                     /gene="rodZ"
                     /locus_tag="JQH22_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFB2207025.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5355..6482
                     /gene="ispG"
                     /locus_tag="JQH22_002767"
                     /gene_synonym="gcpE"
     CDS             5355..6482
                     /gene="ispG"
                     /locus_tag="JQH22_002767"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFB2207026.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6585..7859
                     /gene="hisS"
                     /locus_tag="JQH22_002768"
     CDS             6585..7859
                     /gene="hisS"
                     /locus_tag="JQH22_002768"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFB2207027.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7877..8497
                     /gene="yfgM"
                     /locus_tag="JQH22_002769"
     CDS             7877..8497
                     /gene="yfgM"
                     /locus_tag="JQH22_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFB2207028.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8508..9686
                     /gene="bamB"
                     /locus_tag="JQH22_002770"
     CDS             8508..9686
                     /gene="bamB"
                     /locus_tag="JQH22_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFB2207029.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9804..11276
                     /gene="der"
                     /locus_tag="JQH22_002771"
     CDS             9804..11276
                     /gene="der"
                     /locus_tag="JQH22_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFB2207030.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11346..11561
                     /locus_tag="JQH22_002772"
     CDS             11346..11561
                     /locus_tag="JQH22_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFB2207031.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11558..12928)
                     /gene="xseA"
                     /locus_tag="JQH22_002773"
     CDS             complement(11558..12928)
                     /gene="xseA"
                     /locus_tag="JQH22_002773"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFB2207032.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13090..14556
                     /gene="guaB"
                     /locus_tag="JQH22_002774"
     CDS             13090..14556
                     /gene="guaB"
                     /locus_tag="JQH22_002774"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFB2207033.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14625..16202
                     /gene="guaA"
                     /locus_tag="JQH22_002775"
     CDS             14625..16202
                     /gene="guaA"
                     /locus_tag="JQH22_002775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFB2207034.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16297..16836)
                     /locus_tag="JQH22_002776"
     CDS             complement(16297..16836)
                     /locus_tag="JQH22_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFB2207035.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16852..17370)
                     /locus_tag="JQH22_002777"
     CDS             complement(16852..17370)
                     /locus_tag="JQH22_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFB2207036.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17681..17872)
                     /locus_tag="JQH22_002778"
     CDS             complement(17681..17872)
                     /locus_tag="JQH22_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HFB2207037.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17890..18042
                     /locus_tag="JQH22_002779"
     CDS             17890..18042
                     /locus_tag="JQH22_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207038.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18224..>19357
                     /gene="pdeF"
                     /locus_tag="JQH22_002780"
     CDS             18224..>19357
                     /gene="pdeF"
                     /locus_tag="JQH22_002780"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFB2207039.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4559 a         4711 c         5261 g         4826 t
ORIGIN      
        1 ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg ggcacgttac
       61 cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg ggttaatcca
      121 ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat ttccggtggc
      181 agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac atttggtggc
      241 aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa gcgtgaaatt
      301 ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat cggtgcggca
      361 agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg
      421 gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga gcgtgccgaa
      481 gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc ccgtgagcag
      541 gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc gcaactggca
      601 ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt tactacgctg
      661 gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg gcggttgcca
      721 ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt tcgcggctgg
      781 gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat ggtcaatgcg
      841 atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct ggatgaaggc
      901 ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga ttatcgacca
      961 ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct ggtgcgctcg
     1021 ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt tgcggcaaag
     1081 ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa tctttcactc
     1141 attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac cgcgtttgct
     1201 cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct tgaacggcct
     1261 ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga agcgcaaccc
     1321 ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg caccagctat
     1381 ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg gatctggact
     1441 ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc cgtaccattg
     1501 ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga agacccgcgc
     1561 ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt gccggaaggt
     1621 gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca gccgccaacg
     1681 ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg gctggatgac
     1741 aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt aaatgtctgg
     1801 ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg cttaccaccg
     1861 gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca gctgacaggc
     1921 gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac tttgccgttg
     1981 caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc gttaactgaa
     2041 cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt gctggtgatg
     2101 gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt tttgtccctg
     2161 attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat agtcaacggc
     2221 cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca acagaacaat
     2281 ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg aacgcggtag
     2341 caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc aaaattgtcg
     2401 gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct gaacacgatg
     2461 gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc gtggtttccg
     2521 tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg accaatccgg
     2581 caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc gaaaacggta
     2641 cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc ttcggcgaag
     2701 gcgaagtgtg cccgcgcacc tgttaataat ttcgtaaatg ccgcgtgcaa acgtggcatc
     2761 cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca ccggggcgtt
     2821 tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat agcatatgtc
     2881 tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca acctgctgga
     2941 tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa ccttccgcgc
     3001 cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg agatgaccga
     3061 catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg caccggaagt
     3121 ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg ttggcgatca
     3181 gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg tctcttcgca
     3241 ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct ttaaccgcaa
     3301 cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg tcggcgcagc
     3361 aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg gcgagccgct
     3421 gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt tcggttttgg
     3481 cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc tggataaact
     3541 gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg acgaaattcg
     3601 tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg ctgcggtgcg
     3661 ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat acgtgatgct
     3721 tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc tgaaagatac
     3781 gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt atggacgcag
     3841 ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt tcaccactat
     3901 tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg cgggcgatgt
     3961 tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca tcgatattaa
     4021 agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa tcttttatga
     4081 atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg ctgactttaa
     4141 ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt tgtcacgagc
     4201 acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc gcggtgtggg
     4261 tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca gcgaatgaat
     4321 actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct gcgtaatgct
     4381 cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct gaaggtttcc
     4441 acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac attcctgcgc
     4501 ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact gctgccaggg
     4561 ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag tttttccctc
     4621 ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct ggtgttgttt
     4681 gtggttatcg gcctgagcgg tgtctggtgg tggcaagacc acaaagctca gcaggaagag
     4741 atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga gcaggggcag
     4801 agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac gccgccagcg
     4861 tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc agcaccagct
     4921 gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga taccgccgcg
     4981 accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc aaccgatcag
     5041 gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt tactgccgat
     5101 tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat gcagcgtaaa
     5161 gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg tgcgccagcc
     5221 gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat cagaactaac
     5281 caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca gacgggtaac
     5341 gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca acacgtattt
     5401 acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc atgaccaata
     5461 cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa cgcgttggcg
     5521 ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc aaactcatca
     5581 aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc attgcgctga
     5641 aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc ggtaatgaag
     5701 agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc cgtattggcg
     5761 ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg acgccgcagg
     5821 cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac ttcgatcagt
     5881 tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat cgtttgctgg
     5941 caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt gcacgcagcg
     6001 gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc ggcgacacgc
     6061 tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc gatattttga
     6121 aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc tgttcgcgtc
     6181 aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa gatatcatca
     6241 ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag gcgctggttt
     6301 ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat ggcgtgcgca
     6361 aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt cgtgcgaaag
     6421 ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa ataataacgt
     6481 gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg aaacattgag
     6541 ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca aaaaacattc
     6601 aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg cagcgcattg
     6661 aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc ttgccgattg
     6721 tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg gttgaaaaag
     6781 agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct gaagggacgg
     6841 cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa cagcgtctgt
     6901 ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat cgtcagttcc
     6961 atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct gaactgatta
     7021 tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact cttgagctga
     7081 attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg gcattccttg
     7141 agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact aacccactgc
     7201 gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct ccggcattag
     7261 gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa ctgctggaga
     7321 gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat tactataacc
     7381 gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg tgtgcaggcg
     7441 gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct gtcggttttg
     7501 cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa tttaaagccg
     7561 atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct gcggctatgg
     7621 cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc aaccacggcg
     7681 gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc gttgctgtgg
     7741 tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg cgctctggtg
     7801 agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta ctgggttaag
     7861 gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag aagcggttaa
     7921 acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg gcgttggcgc
     7981 actgattggc tggcgctact ggaacagcca tcaggttgat tctgcacgct ccgcttctct
     8041 tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca tcccggcggc
     8101 ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt tggaacttgc
     8161 gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac aacaggggct
     8221 ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg ctcgcgttca
     8281 ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca aaggtgaagg
     8341 gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag gtgataagca
     8401 aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg cactgagcga
     8461 aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg caattgcgta
     8521 aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg ctgtttaaca
     8581 gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag tttacgccga
     8641 ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc aatcttcatc
     8701 cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta aaagcgctga
     8761 atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat ggctggttct
     8821 ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat gtctacattg
     8881 gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg gcatggcaaa
     8941 ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg gtgttaatcc
     9001 acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc aaatggacag
     9061 ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca acggcttttg
     9121 gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg gaacagggcc
     9181 agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt gaccgtctga
     9241 gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg gcctataatg
     9301 gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc gaactgggtt
     9361 cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa aatgaccggg
     9421 tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat ctgctgcatc
     9481 gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac agtgaaggtt
     9541 atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa gttgatagtt
     9601 ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag gcaaaagacg
     9661 gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa acggctcctg
     9721 gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt tgtctgatga
     9781 tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg cgccctaacg
     9841 taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg gttgcggatt
     9901 tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc cgtgagttta
     9961 tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc cgcatggcgg
    10021 aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg gatgcgcgcg
    10081 cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt gaaaaaccga
    10141 ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg gttgatttct
    10201 actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt ggcgtattaa
    10261 gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa gaggaagtcg
    10321 acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa gaagaagagg
    10381 aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt cgtccgaacg
    10441 taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt gtttacgaca
    10501 tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga cgtgagtatg
    10561 tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct gtagagaaat
    10621 tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg ttagtgattg
    10681 atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt ctcaatagtg
    10741 ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa gtgaaagagc
    10801 aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt gtgcacttta
    10861 tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt gaagcgtatg
    10921 acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg atggctgttg
    10981 aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat gcccacgctg
    11041 gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac ctgcctgatt
    11101 cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg ggatcgccga
    11161 ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac accctgacgc
    11221 caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat aaataattat
    11281 ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct accggaggaa
    11341 ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat aacggccatg
    11401 cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca gactgccatc
    11461 aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag cacgggcatg
    11521 gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt ttacgcgatt
    11581 tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc agacgtgtgg
    11641 ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg tcagtagcag
    11701 tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg aggtgcgtta
    11761 ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt tctgctaaac
    11821 gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt ggattttgct
    11881 gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc tgattttcca
    11941 gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt gccagccgga
    12001 gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt gtacggttgg
    12061 cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc tggcgcagta
    12121 actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc gcacgcagat
    12181 cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg acgaccggaa
    12241 tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat aaatcttcca
    12301 gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga ttcgccagtt
    12361 caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg gggtagatga
    12421 tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt agcgcagcac
    12481 cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt tttttgtatt
    12541 gctgatcgaa caaaccttca gcctgcaact tcgctttgag ctgttcgtac ttctgttgca
    12601 gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg ccgcgcggct
    12661 cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga cggaaggtga
    12721 cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg tctttgagtg
    12781 taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg ctgatccaaa
    12841 cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga ctaacggtaa
    12901 aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa tcagcaggtt
    12961 attcagtcga tagtaacccg cccttcgggg atagcaagca ttttttgcaa aaaggggtag
    13021 atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac tctggtcgag
    13081 atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt tctcctcgtt
    13141 cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct gacgaaaact
    13201 attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga agcgcgcctg
    13261 gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat gtccattgaa
    13321 cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt gactgatccg
    13381 cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga gcgtaacggt
    13441 tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat caccggtcgt
    13501 gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac gccgaaagag
    13561 cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa aatgcacgaa
    13621 aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg catgatcacc
    13681 gtgaaagact tccagaaagc ggaacgtaaa ccgaacgcct gtaaagacga gcaaggccgt
    13741 ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt tgacgcgctg
    13801 gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc agaaggcgtt
    13861 ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat cggcggcaac
    13921 gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc ggttaaagtc
    13981 ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg tgttccgcag
    14041 atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc ggttatcgca
    14101 gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg cgcaagcgcg
    14161 gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat cgaactctac
    14221 cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat gtccaaaggt
    14281 tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc ggaaggtatc
    14341 gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca gatgggcggc
    14401 ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg tactaaagcg
    14461 gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga cgtgaccatt
    14521 actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga tttcatgtcg
    14581 ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg gaaaacattc
    14641 ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg gttgcgcgcc
    14701 gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca gaagcacaaa
    14761 ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt actactgaag
    14821 aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata ttcggcgttt
    14881 gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc tctaacgaac
    14941 gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt cgcggtatcg
    15001 aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc cacggcgata
    15061 aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc tgcccgtttg
    15121 ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg gaagtgactc
    15181 acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc cagtgtgaag
    15241 ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc gagcaggtag
    15301 gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta accgcaatgc
    15361 tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac ggcctgctgc
    15421 gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt cttaacattg
    15481 ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac gatccggaag
    15541 caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa gcgctgaaac
    15601 tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc gaatctgcgg
    15661 cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc ggcctgccga
    15721 aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac gaagtgcgta
    15781 agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg ttcccgggac
    15841 caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac ctgctgcgcc
    15901 gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac aaagtcagcc
    15961 aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat ggtcgtaagt
    16021 atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc gcacactggg
    16081 cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat gaagtgaacg
    16141 gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt gagtgggaat
    16201 gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag ggttttttat
    16261 cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc tgtccgattt
    16321 gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag tcatatttct
    16381 gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct tcagttcctg
    16441 atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg ccagtttttg
    16501 tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt ttttgtccgc
    16561 agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg ccgttgccgc
    16621 tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct ggatttccct
    16681 gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac cttgctgttc
    16741 cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg gatagcttaa
    16801 ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt attagctctt
    16861 ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg taataacaac
    16921 ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat acactttggt
    16981 gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca ccatagaacc
    17041 cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg aattgctgcc
    17101 agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg cgccgaaggc
    17161 ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa gaatggaaat
    17221 aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat aaacatcggc
    17281 ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta acattgccac
    17341 aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt acgaaaaata
    17401 agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg gatagattaa
    17461 atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa catatatatt
    17521 aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa agacgtaaaa
    17581 tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc atccgacagc
    17641 gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt accggtgatt
    17701 cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc caggcaccga
    17761 cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt acgatacggg
    17821 tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc acaaaaggtt
    17881 ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca ggacaaaatg
    17941 gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt tatggaagtt
    18001 aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa acgtaataat
    18061 tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata tgacacttag
    18121 aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg atgcctgtct
    18181 gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac tgaatgcaac
    18241 ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt ctttaatctt
    18301 gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg tttttctctt
    18361 ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg cggcattgcc
    18421 agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt acgaaacgct
    18481 ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt acagggtctt
    18541 tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc agcgtatatt
    18601 ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg ctgcatttgt
    18661 aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc tcgggacttt
    18721 aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt gctacttcat
    18781 cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat taaaacagca
    18841 ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat taggtgcttt
    18901 attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca ccaattatac
    18961 cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt ataagctgat
    19021 ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa attacattcc
    19081 catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc tggtattctc
    19141 ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag tcagacgcat
    19201 tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg ccctgaatcg
    19261 cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg gcatggaaat
    19321 gctggttaag aactatggca tcatgctgcg gattcaa
//
LOCUS       DADLDT020000084        19261 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000084 DADLDT020000000
VERSION     DADLDT020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.084"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            32..331
                     /locus_tag="JQH22_002781"
     CDS             32..331
                     /locus_tag="JQH22_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFB2207040.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="JQH22_002782"
     CDS             328..2076
                     /locus_tag="JQH22_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFB2207041.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="JQH22_002783"
     CDS             2365..2643
                     /locus_tag="JQH22_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207042.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="JQH22_002784"
     CDS             2640..2945
                     /locus_tag="JQH22_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207043.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="JQH22_002785"
     CDS             2955..3116
                     /locus_tag="JQH22_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFB2207044.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="JQH22_002786"
     CDS             3258..3482
                     /locus_tag="JQH22_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207045.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="JQH22_002787"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="JQH22_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="JQH22_002788"
     CDS             4980..5552
                     /locus_tag="JQH22_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFB2207046.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="JQH22_002789"
     CDS             5554..6765
                     /locus_tag="JQH22_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFB2207047.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="JQH22_002790"
     CDS             6762..7100
                     /locus_tag="JQH22_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFB2207048.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="JQH22_002791"
     CDS             7097..7393
                     /locus_tag="JQH22_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFB2207049.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="JQH22_002792"
     CDS             7393..7833
                     /locus_tag="JQH22_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFB2207050.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="JQH22_002793"
     CDS             7817..7999
                     /locus_tag="JQH22_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207051.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="JQH22_002794"
     CDS             8055..8345
                     /locus_tag="JQH22_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFB2207052.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="JQH22_002795"
     CDS             8365..10026
                     /locus_tag="JQH22_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFB2207053.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="JQH22_002796"
     CDS             10040..10321
                     /locus_tag="JQH22_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207054.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="JQH22_002797"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="JQH22_002797"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFB2207055.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="JQH22_002798"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="JQH22_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFB2207056.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="JQH22_002799"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="JQH22_002799"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFB2207057.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="JQH22_002800"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="JQH22_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFB2207058.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="JQH22_002801"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="JQH22_002801"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFB2207059.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="JQH22_002802"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="JQH22_002802"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFB2207060.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="JQH22_002803"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="JQH22_002803"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFB2207061.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="JQH22_002804"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="JQH22_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFB2207062.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5158 g         4359 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttacgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 t
//
LOCUS       DADLDT020000085        19163 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000085 DADLDT020000000
VERSION     DADLDT020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19163
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.085"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH22_002805"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH22_002805"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFB2207063.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH22_002806"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH22_002806"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFB2207064.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH22_002807"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH22_002807"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFB2207065.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="JQH22_002808"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="JQH22_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFB2207066.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH22_002809"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH22_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFB2207067.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH22_002810"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH22_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFB2207068.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH22_002811"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH22_002811"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFB2207069.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="JQH22_002812"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="JQH22_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFB2207070.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="JQH22_002813"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="JQH22_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFB2207071.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="JQH22_002814"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="JQH22_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFB2207072.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="JQH22_002815"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="JQH22_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFB2207073.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="JQH22_002816"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="JQH22_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFB2207074.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQH22_002817"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQH22_002817"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFB2207075.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..>19163
                     /gene="clcA"
                     /locus_tag="JQH22_002818"
     CDS             17859..>19163
                     /gene="clcA"
                     /locus_tag="JQH22_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFB2207076.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLL"
BASE COUNT         4200 a         4885 c         5478 g         4600 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta tta
//
LOCUS       DADLDT020000086        17570 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000086 DADLDT020000000
VERSION     DADLDT020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.086"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JQH22_002819"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JQH22_002819"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFB2207077.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JQH22_002820"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JQH22_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFB2207078.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JQH22_002821"
     CDS             complement(2649..3200)
                     /locus_tag="JQH22_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HFB2207079.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JQH22_002822"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JQH22_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFB2207080.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JQH22_002823"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JQH22_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFB2207081.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JQH22_002824"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JQH22_002824"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFB2207082.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JQH22_002825"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JQH22_002825"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFB2207083.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JQH22_002826"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JQH22_002826"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFB2207084.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JQH22_002827"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JQH22_002827"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFB2207085.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JQH22_002828"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JQH22_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HFB2207086.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JQH22_002829"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JQH22_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFB2207087.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JQH22_002830"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JQH22_002830"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFB2207088.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JQH22_002831"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JQH22_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFB2207089.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JQH22_002832"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JQH22_002832"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFB2207090.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..>17570)
                     /gene="pyrB"
                     /locus_tag="JQH22_002833"
     CDS             complement(16820..>17570)
                     /gene="pyrB"
                     /locus_tag="JQH22_002833"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFB2207091.1"
                     /translation="SMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMR
                     HPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDL
                     KYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAE
                     VDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDK
                     TPHAWYFQQAGNGIFARQALLALVLNRDLVL"
BASE COUNT         4095 a         4477 c         4721 g         4277 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa
//
LOCUS       DADLDT020000087        17377 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000087 DADLDT020000000
VERSION     DADLDT020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17377
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.087"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            131..574
                     /locus_tag="JQH22_002834"
     CDS             131..574
                     /locus_tag="JQH22_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2207092.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="JQH22_002835"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="JQH22_002835"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFB2207093.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQH22_002836"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQH22_002836"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFB2207094.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQH22_002837"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQH22_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFB2207095.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQH22_002838"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQH22_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2207096.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="JQH22_002839"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="JQH22_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFB2207097.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="JQH22_002840"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="JQH22_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFB2207098.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="JQH22_002841"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="JQH22_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFB2207099.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="JQH22_002842"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="JQH22_002842"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFB2207100.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="JQH22_002843"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="JQH22_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFB2207101.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="JQH22_002844"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="JQH22_002844"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFB2207102.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="JQH22_002845"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="JQH22_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFB2207103.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="JQH22_002846"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="JQH22_002846"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFB2207104.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQH22_002847"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQH22_002847"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFB2207105.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="JQH22_002848"
     CDS             13934..14314
                     /locus_tag="JQH22_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFB2207106.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="JQH22_002849"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="JQH22_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFB2207107.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="JQH22_002850"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="JQH22_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFB2207108.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQH22_002851"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQH22_002851"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFB2207109.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4431 a         4539 c         4316 g         4091 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatatta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctg
//
LOCUS       DADLDT020000088        17173 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000088 DADLDT020000000
VERSION     DADLDT020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.088"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..185
                     /locus_tag="JQH22_002852"
     CDS             <1..185
                     /locus_tag="JQH22_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFB2207110.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="JQH22_002853"
     CDS             complement(210..401)
                     /locus_tag="JQH22_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFB2207111.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="JQH22_002854"
     CDS             complement(506..742)
                     /locus_tag="JQH22_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFB2207112.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="JQH22_002855"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="JQH22_002855"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFB2207113.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="JQH22_002856"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="JQH22_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="JQH22_002857"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="JQH22_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFB2207114.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="JQH22_002858"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="JQH22_002858"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFB2207115.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="JQH22_002859"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="JQH22_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFB2207116.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="JQH22_002860"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="JQH22_002860"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFB2207117.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="JQH22_002861"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="JQH22_002861"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFB2207118.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="JQH22_002862"
     CDS             complement(10860..12233)
                     /locus_tag="JQH22_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207119.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="JQH22_002863"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="JQH22_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFB2207120.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="JQH22_002864"
     CDS             complement(13344..13583)
                     /locus_tag="JQH22_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207121.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="JQH22_002865"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="JQH22_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFB2207122.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="JQH22_002866"
     CDS             13960..14247
                     /locus_tag="JQH22_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFB2207123.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="JQH22_002867"
     CDS             14826..14969
                     /locus_tag="JQH22_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HFB2207124.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="JQH22_002868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="JQH22_002868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFB2207125.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="JQH22_002869"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="JQH22_002869"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFB2207126.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="JQH22_002870"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="JQH22_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFB2207127.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4155 c         4337 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DADLDT020000089        17051 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000089 DADLDT020000000
VERSION     DADLDT020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17051
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.089"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..213)
                     /locus_tag="JQH22_002871"
     CDS             complement(<1..213)
                     /locus_tag="JQH22_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFB2207128.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JQH22_002872"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JQH22_002872"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFB2207129.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JQH22_002873"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JQH22_002873"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFB2207130.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JQH22_002874"
     CDS             2894..3634
                     /locus_tag="JQH22_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFB2207131.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JQH22_002875"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JQH22_002875"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFB2207132.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="JQH22_002876"
     CDS             complement(4192..4794)
                     /locus_tag="JQH22_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFB2207133.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4863..4928
                     /gene="yobI"
                     /locus_tag="JQH22_002877"
     CDS             4863..4928
                     /gene="yobI"
                     /locus_tag="JQH22_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFB2207134.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5067..5678
                     /gene="ruvA"
                     /locus_tag="JQH22_002878"
     CDS             5067..5678
                     /gene="ruvA"
                     /locus_tag="JQH22_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFB2207135.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5687..6697
                     /gene="ruvB"
                     /locus_tag="JQH22_002879"
     CDS             5687..6697
                     /gene="ruvB"
                     /locus_tag="JQH22_002879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFB2207136.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="JQH22_002880"
     CDS             complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="JQH22_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFB2207137.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="JQH22_002881"
     CDS             complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="JQH22_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFB2207138.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8460..9392
                     /gene="znuA"
                     /locus_tag="JQH22_002882"
     CDS             8460..9392
                     /gene="znuA"
                     /locus_tag="JQH22_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFB2207139.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9408..10730
                     /gene="mepM"
                     /locus_tag="JQH22_002883"
     CDS             9408..10730
                     /gene="mepM"
                     /locus_tag="JQH22_002883"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFB2207140.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10850..11821
                     /gene="lpxM"
                     /locus_tag="JQH22_002884"
                     /gene_synonym="msbB"
     CDS             10850..11821
                     /gene="lpxM"
                     /locus_tag="JQH22_002884"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFB2207141.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="JQH22_002885"
     CDS             complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="JQH22_002885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFB2207142.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="JQH22_002886"
                     /gene_synonym="yebK"
     CDS             complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="JQH22_002886"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFB2207143.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14728..16203
                     /gene="zwf"
                     /locus_tag="JQH22_002887"
     CDS             14728..16203
                     /gene="zwf"
                     /locus_tag="JQH22_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFB2207144.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16438..>17051
                     /locus_tag="JQH22_002888"
     CDS             16438..>17051
                     /locus_tag="JQH22_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFB2207145.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDR"
BASE COUNT         4352 a         4205 c         4433 g         4061 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaattatttt tcaggagtcg
     4861 caatgtatat ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc
     4921 caatataacc tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc
     4981 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatc
     5041 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
     5101 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
     5161 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
     5221 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt
     5281 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg
     5341 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
     5401 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
     5461 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
     5521 ctagcccggc gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct
     5581 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac accagcagtg
     5641 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
     5701 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
     5761 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
     5821 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
     5881 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
     5941 gacttctggt ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga
     6001 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     6061 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     6121 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     6181 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     6241 ggtgccggat ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag
     6301 tgatgacggt gcgcaggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     6361 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     6421 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     6481 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     6541 cctggcggca gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt
     6601 gattcagcaa ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg
     6661 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     6721 atccgacact ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag
     6781 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     6841 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     6901 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     6961 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     7021 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     7081 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt
     7141 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     7201 ccagaacaaa atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg
     7261 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     7321 cagactaacg accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     7381 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     7441 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     7501 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     7561 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     7621 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     7681 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     7741 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc
     7801 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     7861 acgccacaat ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca
     7921 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat
     7981 gctcgcgcta atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     8041 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     8101 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     8161 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     8221 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     8281 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     8341 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     8401 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     8461 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     8521 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     8581 ccattgctga tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg
     8641 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     8701 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     8761 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg
     8821 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     8881 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     8941 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     9001 aggcacaatt ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     9061 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     9121 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     9181 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     9241 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     9301 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     9361 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     9421 cccgctctgt cgccttggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     9481 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg
     9541 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     9601 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     9661 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     9721 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caacttgctg
     9781 ctgccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     9841 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     9901 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     9961 acaatctgct gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt
    10021 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
    10081 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
    10141 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa
    10201 tccgcgctga ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
    10261 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta
    10321 atccggtgac aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta
    10381 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
    10441 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
    10501 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
    10561 ataccggacg ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg
    10621 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
    10681 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
    10741 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgtgcc
    10801 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
    10861 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
    10921 gggagcatgg ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
    10981 tgatcccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
    11041 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
    11101 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
    11161 aatacgcggg ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga
    11221 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
    11281 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
    11341 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
    11401 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
    11461 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
    11521 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt
    11581 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
    11641 accgatggat gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga
    11701 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
    11761 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
    11821 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
    11881 attcgaaatc tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc
    11941 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
    12001 atcacgtcgc cttgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
    12061 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
    12121 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
    12181 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
    12241 atggtaatga tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca
    12301 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
    12361 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
    12421 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
    12481 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
    12541 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
    12601 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
    12661 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
    12721 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
    12781 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
    12841 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
    12901 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
    12961 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
    13021 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
    13081 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
    13141 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
    13201 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
    13261 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
    13321 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
    13381 agccttttgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
    13441 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
    13501 ttaggacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
    13561 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
    13621 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
    13681 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
    13741 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc
    13801 cagctgcgcc agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac
    13861 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac
    13921 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga
    13981 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
    14041 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
    14101 tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
    14161 gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
    14221 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
    14281 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
    14341 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
    14401 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
    14461 attacgcctg tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat
    14521 aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc
    14581 cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa
    14641 gctcgtaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac
    14701 cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac
    14761 ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat
    14821 caactggaaa aagtcggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct
    14881 gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa
    14941 gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc
    15001 gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt
    15061 atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt
    15121 ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg
    15181 tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag
    15241 gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt
    15301 tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt
    15361 accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag
    15421 atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg
    15481 ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg
    15541 cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc
    15601 ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc
    15661 aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg
    15721 ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat
    15781 ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa
    15841 ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct
    15901 ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc
    15961 tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt
    16021 attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc
    16081 attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg
    16141 ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag
    16201 taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt
    16261 agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac
    16321 actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct
    16381 ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg
    16441 aatccacaat tgttacgcgt aacaaatcga atcattgaac gttcgcgcga gactcgctct
    16501 gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca
    16561 tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa
    16621 agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac
    16681 cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt
    16741 ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga
    16801 atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc
    16861 cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg
    16921 acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg
    16981 gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa
    17041 gtggaccgca t
//
LOCUS       DADLDT020000090        16905 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000090 DADLDT020000000
VERSION     DADLDT020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.090"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="JQH22_002889"
     CDS             complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="JQH22_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFB2207146.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1174..1875
                     /locus_tag="JQH22_002890"
     CDS             1174..1875
                     /locus_tag="JQH22_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFB2207147.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1898..2062
                     /locus_tag="JQH22_002891"
     CDS             1898..2062
                     /locus_tag="JQH22_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207148.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2274..3584)
                     /locus_tag="JQH22_002892"
     CDS             complement(2274..3584)
                     /locus_tag="JQH22_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFB2207149.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3612..4943)
                     /locus_tag="JQH22_002893"
     CDS             complement(3612..4943)
                     /locus_tag="JQH22_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HFB2207150.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="JQH22_002894"
     CDS             complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="JQH22_002894"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFB2207151.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6654..7043)
                     /locus_tag="JQH22_002895"
     CDS             complement(6654..7043)
                     /locus_tag="JQH22_002895"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFB2207152.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="JQH22_002896"
     CDS             complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="JQH22_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFB2207153.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="JQH22_002897"
     CDS             complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="JQH22_002897"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFB2207154.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="JQH22_002898"
     CDS             complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="JQH22_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFB2207155.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="JQH22_002899"
     CDS             complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="JQH22_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFB2207156.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="JQH22_002900"
     CDS             complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="JQH22_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFB2207157.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14188..14532
                     /gene="tdcR"
                     /locus_tag="JQH22_002901"
     CDS             14188..14532
                     /gene="tdcR"
                     /locus_tag="JQH22_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFB2207158.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14788..15327
                     /locus_tag="JQH22_002902"
     CDS             14788..15327
                     /locus_tag="JQH22_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207159.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15349..15696
                     /locus_tag="JQH22_002903"
     CDS             15349..15696
                     /locus_tag="JQH22_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207160.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15865..16515
                     /locus_tag="JQH22_002904"
     CDS             15865..16515
                     /locus_tag="JQH22_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207161.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         4657 a         4313 c         3766 g         4169 t
ORIGIN      
        1 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc agcctacaaa
       61 ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag cgcatcaggc
      121 agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata gattattttc
      181 cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      241 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      301 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      361 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      421 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      481 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      541 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      601 gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg
      661 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      721 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      781 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      841 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
      901 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      961 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
     1021 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1081 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1141 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1201 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1261 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1321 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1381 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggagag
     1441 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1501 aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga gaatccgctg
     1561 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1621 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1681 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     1741 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     1801 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1861 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cgaccaaaaa
     1921 gcgccagcca gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1981 ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa cgcgtttagc
     2041 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg ccttatccgg
     2101 cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata agatgcgcca
     2161 gcatcgtatc cggcatcagg tacaaaacac aacaacatca acaagttgag caccccgtag
     2221 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2281 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2341 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2401 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2461 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2521 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2581 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2641 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2701 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttattgcagt gatcccctgg
     2761 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     2821 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     2881 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     2941 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaatgggact
     3001 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggcgac
     3061 tcttgctcgc cctctgccac acacgcctgg tgggtaaaca ccacaccatt gtgcgtctca
     3121 atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3181 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacagagact
     3241 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3301 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3361 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3421 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3481 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3541 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3601 tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa accagtgacg
     3661 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3721 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3781 aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa aatcatgatg
     3841 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     3901 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     3961 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4021 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4081 tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4141 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat tgatttttcc
     4201 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat cgacgtcagg
     4261 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4321 acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac taccagcagc
     4381 ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4441 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4501 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4561 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4621 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4681 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4741 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4801 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     4861 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     4921 cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     4981 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5041 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta aaataacgga
     5101 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5161 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5221 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5281 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5341 ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa tggcattacg
     5401 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5461 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5521 taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5581 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5641 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5701 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5761 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     5821 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac acggcacatt
     5881 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     5941 ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6001 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6061 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6121 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6181 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6241 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6301 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6361 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6421 tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6481 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6541 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6601 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6661 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6721 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6781 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     6841 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acgtgcctga
     6901 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     6961 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7021 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7081 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7141 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7201 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7261 atacccatcc agcaggccaa caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7321 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7381 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7441 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7501 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7561 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7621 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7681 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7741 cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac gatcgtgcag
     7801 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     7861 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     7921 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     7981 ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag cgcgtgcacc
     8041 aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa tcgcgtaatc
     8101 gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg aagaggtgac
     8161 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8221 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8281 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8341 gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg
     8401 gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc tgctcattga ggacgccagc
     8461 tttaaagtcg cgctcaatgt agatatccag gaacgatgcc gtgcgcccca gcgacatcgc
     8521 gccgccattt tgcgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8581 ctgtgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8641 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8701 atcctcgcct ttttccagac gagactggag atcggcaaat tgcagttcgc gttcacgcac
     8761 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacgacc
     8821 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     8881 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     8941 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9001 gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc aggccaacaa ttttttccag
     9061 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9121 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattacct tttcccacaa
     9181 ttccgtggtg gcaggcgtcg cttcggcgag aaaagattca tcgccttcat acggtgtata
     9241 gttatgttga ataaaatcgc ggacattaat ttcgcttttc cagtccgtac ctttaaagcc
     9301 aagccatgcg tcagcgtaca gcttatcgct ggtatcaata tctaccttca tgaaaaataa
     9361 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttacctaa
     9421 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacga cgcaaatgac
     9481 atgcgcattt tcactggaaa caattcgctc gccaaaagag ttagatcgat tattcatttc
     9541 tgtatcaatc tctacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9601 tgaattctct cctattccgc cggtgaatat aattccatcc agccgacgta atgaagctgc
     9661 atgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9721 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9781 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
     9841 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat
     9901 caagccttcc agcggcgtca ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc
     9961 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10021 attcagcagc gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10081 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10141 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10201 ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga
    10261 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10321 gccgccgtga gcgatgcggt ggccaattaa ggccacgctg tcatttaaat tccgtttttc
    10381 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10441 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10501 cattaatact tcacaatcgc tggcatcgag tacggaaaac ttaatcgaag acgaaccaca
    10561 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10621 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10681 tatccagacg accacggtat ttcgccagag acggcgcttt acggatagca tacatcggca
    10741 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10801 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgagagtat
    10861 tcagtttacc cagcgacact ttggttttgt cacctttata accgaacttc agaatcagac
    10921 cattcaagcc ttccagcgtc cccagatagt gaccgaagaa agatttgaag atagccacga
    10981 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttc gtaccggtca
    11041 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11101 ccatgttggc cggagacaga gtaaacaggc agctaaaggc aaagaacatc accactgcaa
    11161 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11221 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11281 agttaaagga gaaaaccatg atggaaatcc ccagccagac agtgatcagg ataccgtcat
    11341 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11401 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11461 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcaacagga
    11521 acagcgccac aaagccgcga ttcagcggtg caaatccgag ctggttttcc cagaacgtca
    11581 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11641 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11701 tgttgccgga agggttagag ccagaaagac acagacgcgc cagcgcccgg tggcaataaa
    11761 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    11821 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    11881 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    11941 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12001 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12061 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12121 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12181 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12241 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12301 ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac gccaataaca cgaatggtcg
    12361 ggttaataga tttaattgcc accgcaatac cagcaattaa accaccacca ccaattggca
    12421 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12481 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa
    12541 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12601 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12661 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12721 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12781 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    12841 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    12901 gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg cagatcgtat gtaatatgca
    12961 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13021 agacaggtgg attatttatg tttaaataaa aacaaacaac taaccaactt ctattaattg
    13081 ccttcgtcta cacccattat aagatgaata ctctttggct aattccacca aaaccgatgc
    13141 tgctttttta atacgataat ttttcgacca taccgcggca tattgtgcca caggtaatgt
    13201 ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa ggtgacgtca tatcacaagg
    13261 aattacagtt aagaaatcag cattgagaac aagattataa attgtcacga ctgagtcggt
    13321 tttaacgatg ttttcaatac tgatgccatt tctttgtaac gtagtaagca gttcgctgta
    13381 gtaccccata ttagtttgtg gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt
    13441 ggtgccggtg catgttcggg acttactggc taccagcaca aactcggact cgaacagcgg
    13501 ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac gtaccaatcg caaaatccag
    13561 tcgaccgtcg cggattgccg gtaagaacga agacagttgc gcttcataca tagaaacctg
    13621 cgctttcggg aacacctctt tgaacttgtt gatcatccct gacataaaag taaaaccaat
    13681 caatgaagga aaaccaaatg agacttccac caccgcttca gaagacatac cgcttatctc
    13741 attaaccata tttttcattt cacgggtaat ggattcggaa cgggagagta acaattgacc
    13801 ggcaggtgtt aatgttacac cggtattttt ccgcaccact aattccacac caaaataatc
    13861 ttcaatatcg ttaatgattt tactgacggc cggttgagtt aaccctaatt cttttgcagc
    13921 cgagccgata gagccacttc taatgacttc ctgaaagact accaggtgct gcgttttcgg
    13981 aagaagaata gtgctcataa cgacctacgt taattacctc attgacggca tgaagtgtat
    14041 caaaatgaaa taaacaggat atgtgcgacc actcacaaat taactttcat tactttccaa
    14101 ggtatcgtta ttacaattaa atatctttaa atatcaacaa gttaaagcat aaaaatcagc
    14161 ataaaccctg atttttatca aaaaaaaatg acggggataa ccatatttta tggcgataac
    14221 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
    14281 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
    14341 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
    14401 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
    14461 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
    14521 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
    14581 caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa gggggatttt
    14641 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
    14701 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
    14761 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
    14821 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
    14881 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa aatttacagt
    14941 ataagtaatg aactccggag agtattatct gcaattactg cattgaattt ctatcaaggc
    15001 gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt catcatagat
    15061 atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg cacttttgca
    15121 ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga atgtattaag
    15181 gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt aacaccccct
    15241 aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt tttttcaccc
    15301 gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat gttccctgtt
    15361 tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct
    15421 gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa actaaaacag
    15481 acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc
    15541 actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg
    15601 tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc ggacaatacc
    15661 tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg caatatcaat
    15721 gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt
    15781 tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat
    15841 tattatgatt tcacagataa acatatggct aatactattc taaatcattt taaattggat
    15901 tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac
    15961 gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat
    16021 atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt
    16081 accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact
    16141 cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc
    16201 aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata
    16261 tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg
    16321 gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag
    16381 ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata
    16441 acacatatga agcatataac tcagagaatc aatattttac cagagaggac tatacgtata
    16501 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct attattaata
    16561 ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa
    16621 actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg
    16681 caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca
    16741 tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt
    16801 gaaactgacc gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt
    16861 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgta
//
LOCUS       DADLDT020000091        16858 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000091 DADLDT020000000
VERSION     DADLDT020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.091"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(77..970)
                     /gene="virB9"
                     /locus_tag="JQH22_002905"
     CDS             complement(77..970)
                     /gene="virB9"
                     /locus_tag="JQH22_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HFB2207162.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
     gene            complement(963..1649)
                     /locus_tag="JQH22_002906"
     CDS             complement(963..1649)
                     /locus_tag="JQH22_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFB2207163.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            complement(1642..1782)
                     /locus_tag="JQH22_002907"
     CDS             complement(1642..1782)
                     /locus_tag="JQH22_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207164.1"
                     /translation="MKYGITLLMLCLLAGCAQRNTQLPPVSGNPEPVNSPAIIQELTP
                     HV"
     gene            complement(1859..2866)
                     /locus_tag="JQH22_002908"
     CDS             complement(1859..2866)
                     /locus_tag="JQH22_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFB2207165.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQVFLGTAGAAAVMVLTYKLAEALAGASAQGAAQALAASGVG
                     SARQSASSAAGAVGDKASQNLNSTGSATASSASTASRATGDARSRSRAAVQRMQERYQ
                     R"
     gene            complement(2884..3168)
                     /locus_tag="JQH22_002909"
     CDS             complement(2884..3168)
                     /locus_tag="JQH22_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFB2207166.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(3180..3896)
                     /locus_tag="JQH22_002910"
     CDS             complement(3180..3896)
                     /locus_tag="JQH22_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFB2207167.1"
                     /translation="MKLKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKL
                     QQWQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLL
                     TGSGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKIS
                     DTLSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLA
                     EQRRKAQAEQMRTAPIADLN"
     gene            complement(3915..6665)
                     /locus_tag="JQH22_002911"
     CDS             complement(3915..6665)
                     /locus_tag="JQH22_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFB2207168.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(6675..6974)
                     /locus_tag="JQH22_002912"
     CDS             complement(6675..6974)
                     /locus_tag="JQH22_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFB2207169.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(6985..7647)
                     /locus_tag="JQH22_002913"
     CDS             complement(6985..7647)
                     /locus_tag="JQH22_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFB2207170.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(7650..7865)
                     /locus_tag="JQH22_002914"
     CDS             complement(7650..7865)
                     /locus_tag="JQH22_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207171.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(7858..8166)
                     /locus_tag="JQH22_002915"
     CDS             complement(7858..8166)
                     /locus_tag="JQH22_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207172.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            8480..9394
                     /locus_tag="JQH22_002916"
     CDS             8480..9394
                     /locus_tag="JQH22_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFB2207173.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            9398..10381
                     /locus_tag="JQH22_002917"
     CDS             9398..10381
                     /locus_tag="JQH22_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207174.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNSLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            10374..10862
                     /locus_tag="JQH22_002918"
     CDS             10374..10862
                     /locus_tag="JQH22_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFB2207175.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            10859..12577
                     /locus_tag="JQH22_002919"
     CDS             10859..12577
                     /locus_tag="JQH22_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HFB2207176.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            complement(13436..13793)
                     /locus_tag="JQH22_002920"
                     /pseudo
     CDS             complement(13436..13793)
                     /locus_tag="JQH22_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            13985..14962
                     /locus_tag="JQH22_002921"
     CDS             13985..14962
                     /locus_tag="JQH22_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2207177.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(15010..15273)
                     /locus_tag="JQH22_002922"
     CDS             complement(15010..15273)
                     /locus_tag="JQH22_002922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207178.1"
                     /translation="MLPEQHAAEARYPIPVEDFHFSIFTAVNNQTVMTSSAEIVLVIE
                     GTATLSHASGESLLLHVGLSAFIPASIGRWLLTTTGKVCRVSC"
     gene            complement(15308..>16858)
                     /locus_tag="JQH22_002923"
     CDS             complement(15308..>16858)
                     /locus_tag="JQH22_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFB2207179.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT         3976 a         4238 c         4627 g         4017 t
ORIGIN      
        1 tcaatctctg cccgcgcccg ctcgcgggct tccgcctcca gttcggcggc ggttttaatg
       61 tcttcctgtt cgttcatcat ttgctctcca gtttcacatc cggtgagacg gtgctgccgt
      121 tcgccacggt gactttcccg taactgccgt tttcaatccc cacgacctgg ctgccgtagc
      181 gcagcacaaa gcgtggagaa agagagcgca gtaccatcac ccggtagtta ccctcggtgc
      241 tgaaggtcgg ggtgatcacc tgctcgctgc cgccgacgat gacggtcggc gacgggatca
      301 gcttctgcgg tgaaaagccg agataggtaa accggccgtc gtcatacgca aaatccgggg
      361 tgatcgactc cgaacctgcc gccaccctgc gggtgtagtt ccagttgcgc ggcgcagtgg
      421 atttcttcag ggcaaactga atgcgttctt tctcctgcgc ggcctgaagc tcctgctgac
      481 gggcgaggcg ggctgcgtcg ttctgcctgc gggtatcgtc ggggtagctg aagcgcacca
      541 caaaggccag atttcccgcc tcgcgatcgt tatcgatcac gttcagcatc aggctgtagt
      601 cgcgctttgt ggtggtcaca aacaggttgg tctgccagtc ctgctgcgtg ggggtgaaaa
      661 tcttctggac ggttttgccg tccgcatccg ttaccggctg cttcaccggc gcggggctaa
      721 tccccacgcg gttacggtcg ggtgtgatcg tccagccctg cggaaaacca gcggtggcgg
      781 tgatcacctc ctcgtcggcg tcaaaaacca gcgtcgtgat gtaacccgcc cgcgtgttaa
      841 tcactgtcgt gttctggctg ttgtagggga tgttctgcat gcggctgtca taggcgctgc
      901 cgcgcggtgt cgccgccccc catgctgacg ctgcggtcag caggagggca cacatcagta
      961 ccttattcat cgcttttctc cttaaaccgc tggtaactgg tgacgatgaa gccgaacggg
     1021 ttcacctcgc gctcggaggc cgtcattttc ttctgcggga aatagcggta ggtcatgcgc
     1081 accgtccagt aatcatacgt ttcggtgttg tccttgacct ttcgaatggt gcgcttgatg
     1141 cgcagcatct ccaccatgct ggagccggtg ccctctgacg gtacgttgga gaggacttcc
     1201 accgtgacta cataggccgc ctgctgaaac acagcgtccg gcgcgtccgg gctgttaaac
     1261 cagtcgaggt attccttgtt gacctcgtcg ctgttgaaca tctgcgtctc tctgtagtcc
     1321 tgctgaaggg cgaaatagtt gtacccctcg cggcgcttca cgtaggacgc agtctgcgag
     1381 agattgagca cctcggaggt gtccagcttg tcctcgccga cggtggtgac gcgctcgatg
     1441 cgcccggtct gacggtcgag cgtgtacagc tccacggtgg tggttttgag cggtagcagc
     1501 aggataaggg cggcaagggc gaagacgagc agcagaccgg cgagcgccgc cacgatccag
     1561 cccgcctttt tcatgcgcat atcccgctca agaatggcgt tctcaaacga gcgcgagtcg
     1621 ctcagggctt tggcggtaag ttcagacatg gggggttaac tcctgaataa tggccgggga
     1681 attgaccggc tccgggttgc ccgacacggg cggaagttgg gtgtttcgct gtgcgcagcc
     1741 tgcaagcagg cacagcatca gcagcgtgat gccgtatttc atggtgtttt tcctgtggta
     1801 aagacgtgac gaagccgcag gacgccgttg cggcgtcccg cagttcagtc agactgggtt
     1861 atctctggta gcgttcctgc atccgctgaa cggcggcgcg gctgcggctg cgcgcatcgc
     1921 cggtggcgcg ggaggccgtg gaggcagacg aggccgttgc gctgccggtg ctgttaaggt
     1981 tctgtgaggc tttatccccc accgcaccgg ctgcggagga ggcggattga cgggctgaac
     2041 ctactcccga cgcggcaagt gcctgagcgg caccctgtgc ggatgcaccg gcaagcgcct
     2101 cggccagctt ataggtcagg accatcacgg cggcggcacc cgccgtgccg aggaagacct
     2161 gaacggccag agtcaggtag ttggcatcgg atgcttttgt ggcgcccgcc aggatgtagt
     2221 ccaggtaatc cattaccacc gtcagggcga tgccgctgaa cagggatgta aggatgcagc
     2281 ccagaataat tctgagccag ttgctgaaca tatcccggaa ccacgaccac atcaggcagt
     2341 agataaagat cggggcggtc gccagcatca gcagcatcac cagttcggct atcaggttaa
     2401 tgatggccgt gatgcctacc agtaacatga cgcctgccca gaccagaaac tgcgccagag
     2461 tgccttttag agcgacatag gttgaatcat cataagagta aagcttctcc ccaagtgctt
     2521 gagccttttc ccagacggta tccagccgcg cccagatcga cgcgtcgccg ctgaatccgt
     2581 cccgcaggcc ggtgatggca tccgaaacca gatcgagata gcctccggcg ttggtgacga
     2641 aaatcatgat gatgagcatc cggctcacgt cccacacgat ctcctcgaag gggcggacta
     2701 gcttgcctgc catcgtctgg taggcgcgcc acgtgatgta cagcgagagc accgcgatgg
     2761 agaacaggtg tgccatccac ccccccgtat cggcggccac gctggtgagc acgctgtcaa
     2821 acccctccat gatgtggctg tttaatccga cgaatattcc actcatggtg cgttctcctg
     2881 tcgctaattc aggtcagcaa taggctcttg cacgcgagct ttatcaaggt ctttcaggat
     2941 ttgcttatat ccttcggcaa catcaggttt agctttttca cggtgaaggg cggagcgtgc
     3001 attctggcaa tcggtgggca caaaattatg attaaaaatg cactccctgt atttcgccag
     3061 catgtcgtca tggtgtgcca tataccagtc tacggaggtt ttatcctcgc agccagtaag
     3121 cgcggccact gaggccagaa caataaaggt cagtgtcttc agtttcatgg ggtcacctgt
     3181 tagttaaggt ctgcgattgg agctgtgcgc atttgctcgg cctgcgcctt tcggcgttgt
     3241 tcggcaagca gctggttgcg catctcactt tgtttgaccg acatttccca ttgagtggtc
     3301 agggcgttca gctggatact ccgcgcggac actacgttcg ccagatcctg cgattcttta
     3361 ctgtccttcg cattctcgat gcgactggcg agattactaa tatcgctcag cgtgtcggaa
     3421 attttttcct ggatgtcaga agtgtcctcg acggacaccg cctgattgag gagcatctgt
     3481 ttgcagctat cccggtacgc ctcgctggcg ttctccgggc agttgtcgaa cagtttgtat
     3541 ttgtcataca gcctgtttag ttccccagca taagacccgc ctgagccggt taacagatcg
     3601 tttaaggaaa tccccctgtt tttcagattt ttaatgtcag ccgtcaggtt ttttgcctga
     3661 ttcaggaatg cctggacgtc ccgaataccg gtggccgttg ccagctgttg tttataagcg
     3721 ttcatctgcg actgataatg ttgaaccgtc tgctgccact gctggagctt ctccgcccac
     3781 tgctggatgt tgctggcgtt ggctacttca tcaaaaacgg gaataccggc ctgagccggg
     3841 acgtgaaaga gacacaaggc gacggtcagc gccgtcaggg tggttttcag tttcatggtg
     3901 ttatcctttt gtgattaaac ggcacgtctc agcatttcgt ctttccagtc ttccggcttc
     3961 ataccctcct gccagatctc gtcaaaaatt ttcaggttgt ccgcactggc gctgagcagc
     4021 ttcgtgtagc gacctagccc ggagaggtcg agggtgattt gtgcgatata cgggcgcacg
     4081 tcaccacgcc gcagcggaga tttcatgatc accatctggc gggcgtaggg gtcgagggtg
     4141 gtcacgatgt cgtaaaccgc tttcggcacc cgcagcatgt ccacgtagtc gtactccttc
     4201 gcctgctggt tcgccatgaa gatgcgggta ctacactgct cgataatggc cgccgagatc
     4261 tcgtgcttgc acatctcctc cggcgactgc gtggccggga agaaaatgcc gttgagcttt
     4321 cgaatgacct tcagcatgtc gagggcgaat tttgaaaact ccgggtcctg aagccatttc
     4381 cagaattcat ccatgaaaat gaccaggcgg cggccatcga ggaggctggt cacgcggtag
     4441 agcaggtaga aggtgagcgg gctgcgcacg tctttgtcgt tcaggaactc cgtgccgtcg
     4501 atgccgaaat tatcaatatt gtcgatatca aaggtgtcca cgtcattatc aaacacccag
     4561 ccaaactccc cgccctgcgc ccactgctgg agacggatgc gcagcccgtt catccgtgcg
     4621 tccttcgtcg gggcttcatg cagcacttcc agcatgcggg tgatgccgtt gcgacgaagc
     4681 tcaggcgggt agtccagcat gatggtgtcc actgcctcgc tgaggcgttc ttcatcgcgc
     4741 gggtcgagcg gtttgccgtc ccttgtgcac atcattcgca tcagcttctt gaggaagttg
     4801 cggttacgtt tcgttggagc gagcgcaaac gggttaaggc cggtcttctc gcctgaacgg
     4861 aaacggaagt acgagccgcc ctggaggcgt atcgacattt ccgccgaacg gtctttatca
     4921 aagaccaccg aggtgaagcg ctttttcgtc gcccccggcg caaacgactc aggacgccgg
     4981 tatttctgca tcagatgctg gaacatcatc agcagggtcg atttccccgt cccggtactg
     5041 ccgagcagca tggtgttacc cggcggcttc tcgctgaact cgtcaacatc ggcctggctg
     5101 ttgtgcagat tcacgtactg acatcccccg gacggggtcg gaacaacggc aatcgcctcc
     5161 ccccaggcat tcccgtcccg cttgtgcggg tgaaagttgt gaaacgacac catttcggcg
     5221 tagttgaggc tgctcaccgg caccagacgc gggcgcaggt gatacacacc cggcaactgc
     5281 gcgagatacg ccgccgacag cgactcgcgg gacagggtca tgatgaaacc gagatccttc
     5341 agggtatccg ccagggtgtt ggtgtcccgg ataacctgct cgctggttgg ggcgctgaca
     5401 ataagcgtct ggtgatattt cccgaatgaa ataatgccgg actgaagcat gtcgcgggcg
     5461 accttcaggt cttcctgctg ggagatcgca tcatcctcgg cagacttcag gcgtttctcg
     5521 atggtgcgga tgcagccccg cgcttcatcc ttacccatgc aggtatagga ctgcgtgaac
     5581 acgtaccggc agcgggcata aagaagcgag tcatacagcc cggtgatggt ttcagtgctg
     5641 taatccttga tttccagccc gcgaaagtag ctctggccgt cggtgtggct gacctggcca
     5701 atgtcggtgg aagggaaaat atccgtgccg cccagcacgt cataaaacgg cgtgcgggtg
     5761 acgcggactt tctgccacct gccggtgatc agaaagttgt aaaacgccag ctgggaggag
     5821 aacacgtgcc cgttctcctc ataagtgcca agcggccgcg ccgcgtagcg tgacagcgcc
     5881 gtgccaatcg cttgccggtg ctcctgcatc acctgcaggg cattgtcgag cagggtttta
     5941 cgttcgccca gggacttctg tttcatggtc gcccgctcaa tgccggtgtg cggattgaag
     6001 cagacggtga agaagaggcg acgctggtag aaaggtttgc cctcgcggga ggcgtaaaac
     6061 agacgcgcga tttcatctga aaacggaatc ccggattcac attcaagcgt gtcggtaaat
     6121 tcgtcgctca ccagatgggt atagaaggta acgggtagcc cctcaaagga gcggataacg
     6181 ttgttgagct gcatgtccag ggtggcgagg taatgttcat cctcgcattc aaaaatgata
     6241 ccgccgatct cccaggtgct gacaaagtca ccttttttgt tcttcaccac ctgctcgtgc
     6301 aggtgggaag accaggggag atagcgggac agcgggatac tctcgttgag ccgcatatgt
     6361 tttataaact ccgtaacgtc tatggcgtcg tactggtttg cgacaagcgc ctttgtcccg
     6421 aaatgccggg tcgccgcgcg gttgccacgc gttttaaacg ccagcaactt caggtcaaag
     6481 aaatgaatgt cctcttttgc cagccgtctc agctcgataa aggcaggtat gagaagcagg
     6541 agaagccagt aaaaacgtga ccagacggcc gccagggtaa ggatgcacag ggtgaacagc
     6601 agggggacaa gcgggacccc ccacagcgcc gccggtcggg tcagcgcctt gttgaggatg
     6661 gtcatgctgc ctccttaggc ggcgaagtag gcaccgaacc cggacgcccc ggcgagcagg
     6721 acacccgccc agaacacgcg ctggagatcg tgccaggcct tgccgtcaac aagcatcttg
     6781 taggcgataa aggccaccgc acagctgata gtgacgactg acataccagt taggccgagg
     6841 aagacccagt taatggcgtc actgcctttc tgaaatgcct ggtcagcgct ggccgccatt
     6901 gctggaacag cagacagggc ggaaaatgcg gcaatcaggc gggttgcagc ggttttgata
     6961 gtacgtaaag tcatgatctc ttccttaatc agaagacaca gcgaggccgc cgcgcacgac
     7021 atgtggcgga taagcagcga tgctggtcgg ggtggtgtcg ggtgccggaa ttacgccgcg
     7081 tatgacgcta tccgggtaaa cagtgttgtc ggacgggacg gcaggcgctt ccccggatga
     7141 gggagatgct gtctgctgtt ctgcccgcgt tgagggcaca acgtagccaa tacgctgtac
     7201 gtagcttgtg ttgccgaacg cgctttccgg gcgctggccg gtttcaaagt tgcctgagta
     7261 atagcagctc agggcgcgct gtaatgttcc tcctcgttga tagcagtcgg tgatgatttt
     7321 cgcggcaaca gtcatgttgt cacaggggtt aaacatggat tcggcgctta cgccgaactg
     7381 cgggaagttg gtgctggtta tctgcatcag ccctactgaa taacggtgct ttttctgctt
     7441 tatggcctcc acaattttta gcgcctcgtc tttcgacgaa ggcagatgag aaatcacgcg
     7501 gctcctgccg ccctttaccg ggacaatctc agcgatggca tatggattta agccggattc
     7561 ggtgcgggtg atgtcgtgga tggtatcggg gtgaacggcg ggagcacact gcattgcgag
     7621 ctgaaacaac gcggcagtgg ttaacatagt cagtcctttc tgtagagcag taccttaccg
     7681 gtggcacagt ccgggttgat aaataactga atgtcgccct ggctgttagc ttgggcaaca
     7741 ccgatttttg taagcgtcat tgccagccga tcacccttca ccgaatacac gttgtgcgtg
     7801 tactcggtca tgggggtgaa tacggcttca agcagtgagg ccggagcagg cgtgatgtca
     7861 ggcattacat ttcctatttt ccagcagtga ggtgattgca tcgatacgtc gcagcctgtc
     7921 ggcaataagt acggatacat cggatttttt gagtccctgc gctttcacag tcagggtagt
     7981 gatttctgcc cgcagggctg cccgttcaat tagcaattct cggacgttat ccggcagctc
     8041 cctgagcgac caggtgccat taatggcacg cgtgatacgg tcacgggagg agcgtagcgg
     8101 cggcttgccg agagttgaat aaggattctg acgcagcgcc ttacggcgga cggtttgcat
     8161 gctcatggct gtttctcctg ggtatgagta agccacccgc gtgcggtgca cagtgggaga
     8221 cagggaagga ttaaacggga ggcttaacga tacagaggag tgagagtgtt acccattaca
     8281 ggcgcggcac gtcatctctt tacgcagtca cagaaaacac ctccttatcc tgaccgggga
     8341 acgccgctgc taggttatta aagagctaat gatttgatta atacaatagt gcttataagt
     8401 tgggtattca ataaaattat aacttatagt atcgatttaa gaatgattcc aatttttagg
     8461 actttttcag ctgtgtaaaa tgttggagat gtttgaaaaa attgaaaaaa aatactggca
     8521 ggataaaatc aagaaaatct ccaaattcag agtaataaaa atgcaagatc tcgatgatgc
     8581 aaaagtgata gaggtactgg cgaaggtatt gaccaaaaaa ggtatttcca aggtaaagca
     8641 caatagttac ataagtaagg tgctacagat cactacatca gcgggctata agaagttgaa
     8701 tggtgcgacc aagtgggagg ttcagcaact cataaaagta gtccaatccg ttggcatgga
     8761 tatgattgag tttttccgta tttattatga ggataataaa gaaattcaag atgcgatctg
     8821 gaacgatggc cgcagtgaac actcttgtaa ggtatgcctc tatcctgaag gttcggaagt
     8881 aaacacagaa tactcagcac tgaaagtagc tgaccaatgg aacatttttc cgacaaatga
     8941 tttaaaagat gaactgcttt atgaatcaaa acgaagcata gaaagtattg tcataaatcc
     9001 aaaggtaatt agcagcacta agtatcgaat tgcactactt gatgacgatg ctaatatcac
     9061 cgaaagctta aaagaaatcc tttcgaacga ttattatcaa gtagatactt atgataactt
     9121 gaaaaaactt actcaagctg tcgcaacaaa tccgtatgac gcatatgttt tggactggat
     9181 catcggtgat gaaacaagct ttaagttggt taaagctata aggtcgtcaa aaaataaaaa
     9241 tgcaatgata atcgttctta caggtcaact atctggtata aaagatgatg aaattgtaaa
     9301 gtcaattcat gattatgaca ttgtgggacc atacgaaaag ccgattaaat caggcatcat
     9361 caaatctaac attgaaaaat atttcgccag atagattatg agaaatttac ttgaatattc
     9421 cggtagcttg ataagtaacg atagtttgat cgtagcacct tacgattgtc agaagctaag
     9481 ctatgaaaat attttaccat taaacaatag tcttataaaa aactggaata tacaaaaatt
     9541 ctcaagtgat tactctgaaa tagaacacct aaatgtgatc aatggcatgg gagtgacatt
     9601 aggtgattct atcgtcggga tttctgtgct gtcagcaatc aaaaaaaaga atcccaagat
     9661 taaaattaag cttattcgac cggagactgc accgtcatat gttgaggaac tgtatgaatt
     9721 agcatcctcg gttatagatg agcttcatta catgcctttt catattcaga aattgccttg
     9781 tactgcattg aatattgatg tgggaaatca gctatattct ccagatttcc atattatgga
     9841 aatgcatgat tattttatcc aaggtcttgg cgtgaaactt gatgatgtta accataatga
     9901 gctatctaat aaatggttag gcaatattga tctcggagcg ccagtttttg aaaactacac
     9961 actttttgca cctattgcta gcacaaaaat aagaagtata ccgtcgaagt tctattatga
    10021 catagttgat atgctttcta ggcgcgaagg aaaaacggta ctaggtttcg tggatgtgaa
    10081 tcataaaaat tatattaaca tatcaaagta ttcaccccat acgaaagatt tcatagcgct
    10141 aattaagtat gctagcaacg tttatacttg tgactcctct gcattgcata tagccgctgg
    10201 atttggtgta cctactgaat gcgtgtttaa tacaataccg ccagaattaa gaacaaagta
    10261 ttacagtaag tgtaagtcga tttatgttgg gaataaaaga ttagaaaaaa ttcagagtag
    10321 tgaagatttc caactggtta aaatggtgga aaataattac agggagtatc tttatggtta
    10381 aaataatatc attcttatcc ctaatgcttt tgtcaattag tgcatatagc aatattgggg
    10441 atatagttat agatggttca atcgctaatg ctaacagtga taaaggttat gtgattactg
    10501 aaaagcagtt tgattcgttc ccacgggata ccatcgtaac gacaacacct tggacgccaa
    10561 ccggtaaaaa agtaaaattc aagggcgtcg acctaagtta tgttttaaaa ctcgctggcg
    10621 ctaatggagc aaagctaaaa ttccatgctc tgaatgatta tgaaatcatt gtagacatga
    10681 atgacgttga aaaatataac attttattgg ctacagaaat ggatggaaaa aggcttcaaa
    10741 taagggattt tggaccttat tttttggtat atcctttaga tgaacattat attgagctta
    10801 acacgccaca ctatcttgca cggttcattt ggcagataga caaaattacg gtgctcaaat
    10861 gaaaaaaaat ttctttatca ttttggttgc tctgcttttg ttgatagcat tcactgtgtc
    10921 tagtgttgtt aagtacacca gtcttttatc ggagaaaaat ttatacaccg tcgctggcac
    10981 ccaagaaaac tacggtttct cagtggcaaa attcattata cagttgaatg agctttacac
    11041 gctaatcaat agcgataata ctattgacga tgttaggctt aaattcgata tcctttattc
    11101 tcgtttgaat gttatttatg taaagagtga ggcgactgca cctctatata aacagcaagg
    11161 ctatgaagaa acaattgatt cgataaacaa aaagcttgaa gacatagacg ggctgctttc
    11221 gcataatcac cctgattata aaaaaataag tacaatcata gctgatataa aaccgctaac
    11281 taaaaccgtt acaaatcttg ctgatatggc tgaaatagca caaaggaatg acgctcttaa
    11341 agatttcagg agcaaacgtc agcagttatg gactctgctt tttattacag gtgggttgat
    11401 ttccttactt ctttttgtcc tatttattta cataagtaaa attaaccgat tgttactatc
    11461 agaacgggct gcttttgcca gtaaaaatgc atttttaggt atggtaggcc atgaactcag
    11521 aacatcttta caggcaatag tgtctatcat tgatgtcgta accaataatc tgagtggagg
    11581 gattaaatct ggtcagatag aacgacttga aactgctgtt tcgaaaatgg agcgccagtt
    11641 aaatgatttg gctgaatttg caaaaattga caacggatct gtagagataa aaaacaccta
    11701 taatagcttg caagcaattg ttactaacgc tgtgcaggat tgcattgcta tttatgaaaa
    11761 aaaggatgtc acagttaaaa taaaaaacaa taatgatgca gtgattttta cagatgctct
    11821 acgccttaat caagtaattg aaaacttaac cagtaacgct attaagtaca ctgagcgcgg
    11881 ggaagttaat gtagattact ttatagaaaa aggtaaggta ttaaatattg tcatttctga
    11941 taccggaaag ggaatcccta aagacaaact aaagttcata tttaaaccat ttacacgagt
    12001 tgtagattcg aaatccactg tacctggatt tgggatggga cttgctattg ttgcagggat
    12061 aatcagacta ttaaaaggta ctattcatat atctagtgaa gtaaatgtag gtacaactgt
    12121 tacagtgaga atacctatca agttgggaga tagcagcctt gcaacagctg aggtatctgc
    12181 ttcagctccc ggagatgtct tcggcgttct tagcttgctt gttattgatg ataatgaaat
    12241 ggcctgttcg tctctttcca gtctgcttac taatgctgga tacattgtcg aggctacaac
    12301 ctcgcctgaa cgagccctgg aaaagcttct gcgtaaacct tatgacgttg tcctgagtga
    12361 tttacaaatg ccaggcatga caggtgatga gctgtatgca actgttagta gcagtcagaa
    12421 tcctaatcga tccacgcctt tcatattcat cagcgcatac agtgatgcac actctatcgg
    12481 acaggctcct ttgttaacta aacctgtcag gttaaaagat ataaacctta aaatacatga
    12541 ggttatgtca aatcaaggac gagtcaaaag tgagtaggca ttgtcgtaat atttgcgatt
    12601 atagctttgg tgttctcgat cgttgcgaat tttgaaaatt tgtggagtca aatggcgccg
    12661 aaagcccggc gtagccggtt acctggctat aaactctgcc accctacccc ctgccctgat
    12721 tagccttaac ccgcttacag caatgaagaa gtaaaagtaa tcccttttaa ccccggtgag
    12781 cgggctttac ggcgtttacg acgtaaaggg cgtgatttcc ggggccttag cggcggcagc
    12841 ttgctgctga cgcttagggg gatgttcttc agttatttca gcgacaccag ggaacttaac
    12901 acggtagttg caaagctata cccgctaaaa atgtctcaga tgcctctgta cgcggtcgga
    12961 gtggtcccgg caaggcgtca gccgcagccg gggcgtatct ccgcgttagc gggccgaagg
    13021 gccgcttacg agcgtgtacc cgacaactga ccgcagctcc acccacagcg atgaagaaga
    13081 gtagttacaa cacccatcat taaaagcgac actactcccg gccttcggcc gggggcggcg
    13141 gcgcttttca gtggtgggga ggggcacccg gccggcaaac cggcgccggt tttatgatga
    13201 gctttctttt gaatgctgta actaatttgg ttctccggtc ggggcccttc gcgaagcgat
    13261 gggagctctt tctgatgttg ccgaaccggc gcgaccgtca ggtcgctgca agagcgggca
    13321 ttctccggag cctgtccaca gggtaggcga tgaagaggtt atgtgtttac aggatttatt
    13381 aaaggatctt taaaagagat acagaactgg tgtttaaaag gaactacgcg ccctgaaaga
    13441 ccgacggcag gctcagaaga tgctccagcg tggccatagt acgttcaccg aagacgtgtg
    13501 caacaaccgt cctccgtatc ctgtcatacg cgtaaaacaa ccagcgctgg cgtgatttag
    13561 cgccgacgta accccactgt tcgtccattt ccgcgcaaac aatgacgtca ctgcccggtt
    13621 gtatgcgtga gtttaccgac tgcggcctga gaggcggtgt aagtgaactg tagctgccat
    13681 gttttacggc agtgagagca gagatagcgc tgatgtccgg cagtactttt accgttacgc
    13741 accacgcctt cagtagctga gcaggaggga caactgatgg agatggaagc cacgggagca
    13801 cctcaaaacc accatcatac actaaatcag taagttggca gcatcaccgt aatcgtacag
    13861 ctaagaacaa gtgcttcaag tctacttatt aaaatgtacc tttgaaaaag tagactttct
    13921 gcggtacgct ctgacagtaa gtttgccccc gattttttac gcagcgcgcc ggacggagcc
    13981 cgacatgatc ctgattcccg atgacgaacc ggagctgagt ctgcccgcag ccagtgagga
    14041 attcctgccg gcgctgtcag gtgagaatgc cccggtcagc ccggcccggg cctacctgct
    14101 ttcactcaat tcccaccgca gccggcagac catggcctcg ttcctgaaca tcgtcgccgt
    14161 tatgctcgga gctgcctccc tggagtcctg cagctggggc agcctgcggc gccatcacgt
    14221 gatggccgtg accgaactgc tgcgcgacac cggccgggcc accgccaccg tcaacaccta
    14281 tctttcggca ctcaagggcg tggcgaagga agcctggatg ctccggctca tggatgtgga
    14341 gagcttccag catatccggg cggtccgtaa cctgcgcggc agtcggctgc ccagcggtcg
    14401 ggcgctgccc cagggggaga tccgcgccct gtttgccgtc tgtgaagccg atcgcagctg
    14461 cctcggggcg cgggatgcag cgatgctggc ggtcattctc ggctgcggcc tgcgacgatc
    14521 cgaagtagtg agcctggatt tgcgtgacgt tgtcactcag gaccgtgcgc ttagagtgct
    14581 gggtaaggga aacaaggagc gactggcata tgtcccggcc ggtgcctggc agcggctgca
    14641 gatctggatc gatgagatcc ggggcgagac cccgggcccg ctgttcacac gcatccgtcg
    14701 tttcggggat gtgactctaa accggctaac cgaccaggcg gtgtaccata tcctgcaggt
    14761 gcgccagggt caggccggca tcacgaaatg ttctccacac gatctgaggc gaaccttcgc
    14821 caccgcgatg ctggataacg gggaggattt aattaccgtg aaggatgcga tgggccacgc
    14881 gagcgtcacc accacccagc agtatgaccg tcgcggggag caacgcctgc aggacgcgcg
    14941 ggatagactc aaccttattt agggctaagt aaacacaaac aagggggctg taccccctct
    15001 aattttacat taacacgata ctcgacagac tttcccagtc gtagtcagca accatcgccc
    15061 gatggaggct gggataaatg ctgacagccc gacgtgaagc aaaaggctct cgccgctggc
    15121 atggcttaat gtcgcggtcc cctcaatcac taacacaatt tcagcgctgg atgtcattac
    15181 cgtttgattg ttcaccgcag tgaaaatact gaaatggaaa tcctcaacag gaataggata
    15241 gcgcgcttct gcggcgtgct gttccggtaa cataggggag cccgcagaat tcggaaaaaa
    15301 tcgtacgcta aggttttccg ggcatccgta agggccgaaa cttcccgtct tccagtctgc
    15361 ggctctgccg ccagacgtaa tcgccggtta ggttgatgtg ctcccagccc agcggcgaca
    15421 ggaattgcag cagctcgccg tccaccggct tgccggcctc gaccaacccc tgggtggcgc
    15481 gttccaggta caccgtgttc cacagcacga tagccgccgt caccaggttg aggccgctgg
    15541 cccggtagcg ctgctgctcg aagctccgat ccctgatttc cccaaggcgg ttgaagaaca
    15601 ccgccctggc cagcgagttg cgcgcctcac ctttgttcag gccggcatgc acgcggcggc
    15661 gcagttcaac actttgcagc cagtccagga tgaacagcgt gcgctcgatc cggcccagct
    15721 cgcgcagggc cacggccagt ccgttctggc gcgggtagct gccgagcttg cgcagcatca
    15781 gcgaggcggt gacggtgccc tgcttgatcg agctggccag gcgcaggatg tcgtcccagt
    15841 gggcacgcac gtgcttgatg ttcagggtgc cgccgatcag cgggcgcaac gtcgggtagg
    15901 cttgcacgcc ctgcggcacg tacagcttgg tttcgccgag gtcgcggatg cgcggcgcga
    15961 agcggaagcc tagcaggtgc atcagggcaa agacgtgatc ggtgaagccg gccgtgtcgg
    16021 tgtagtgctc ctcgatccgc aggtcggact cgtggtacag caggccgtcg agcacatagg
    16081 tggaatcgcg gacgccgaca ttcaccacgc gggtgctgaa cggcgcgtac tggtcggaga
    16141 tatgggtata gaacagccgt cccggctcgc taccgtactt cgggttgacg tgcccggtgc
    16201 tctcgccccg gccacccgcg cggaagcgct ggccatcgga ggatgaggtc gtgccgtcgc
    16261 cccagtgggc ggcaaaggcg tggcgatact ggtggttgac cagctcggcc aaggccgccg
    16321 aataggtttc gtcgcggatg tgccaggctt gcagccagga cagcttggcg taggtcaggc
    16381 cggggctcga ctcggccatc ttggtcagcc cgaggttgat cgcatcaccg aggattgcgg
    16441 acagcagcaa cgtcctgtct ttggcctcgg ccccgtcctt caagtgggtg aagtggcggc
    16501 tgaagcccgt ccagtcgtcc acgtccatca gcagttcggt gatcttgatg cgcggcagta
    16561 actggctggt ttggtcgatc agcgcctgcg cccgatccgg caccgccgca tccagcgggg
    16621 tgattttcag ccctgactcg gtgaggatgg catcgggcag ctcgttgtcc ttggccaggc
    16681 gggtgacggt ggccaactgc tcgtccagca gctgcaaacg ctcttccagg tactggtcgc
    16741 tgttcgggtt gatcgccagg ggcagggcct gctcgcgctt gagtgcggcg aacttctcgg
    16801 ccggcagcag gtagtcgtcg aagtcgcgga actgccgcga gcccttgacc cagatgtc
//
LOCUS       DADLDT020000092        16498 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000092 DADLDT020000000
VERSION     DADLDT020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16498
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.092"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            353..1711
                     /locus_tag="JQH22_002924"
     CDS             353..1711
                     /locus_tag="JQH22_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFB2207180.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="JQH22_002925"
     CDS             complement(1950..3335)
                     /locus_tag="JQH22_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFB2207181.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JQH22_002926"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JQH22_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFB2207182.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="JQH22_002927"
     CDS             complement(3729..4109)
                     /locus_tag="JQH22_002927"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HFB2207183.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="JQH22_002928"
     CDS             complement(4464..4898)
                     /locus_tag="JQH22_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFB2207184.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="JQH22_002929"
     CDS             complement(4886..5287)
                     /locus_tag="JQH22_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207185.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="JQH22_002930"
     CDS             complement(5547..6116)
                     /locus_tag="JQH22_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFB2207186.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7004)
                     /locus_tag="JQH22_002931"
                     /pseudo
     CDS             complement(6861..7004)
                     /locus_tag="JQH22_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7338..7604)
                     /locus_tag="JQH22_002932"
     CDS             complement(7338..7604)
                     /locus_tag="JQH22_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207187.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="JQH22_002933"
     CDS             7745..7951
                     /locus_tag="JQH22_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HFB2207188.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="JQH22_002934"
     CDS             8046..8648
                     /locus_tag="JQH22_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207189.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..10765
                     /locus_tag="JQH22_002935"
     CDS             9848..10765
                     /locus_tag="JQH22_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207190.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISSMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="JQH22_002936"
     CDS             10850..11722
                     /locus_tag="JQH22_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFB2207191.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            11940..14324
                     /locus_tag="JQH22_002937"
     CDS             11940..14324
                     /locus_tag="JQH22_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFB2207192.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            14321..15226
                     /locus_tag="JQH22_002938"
     CDS             14321..15226
                     /locus_tag="JQH22_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFB2207193.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            15223..16293
                     /locus_tag="JQH22_002939"
     CDS             15223..16293
                     /locus_tag="JQH22_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFB2207194.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         4023 a         4244 c         4325 g         3906 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggggt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctag tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgggctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt aaggttctgc gcgctgttcg tgacaccgtg gtctctgttg cccgcgcggt
    11701 atggaactgg attttcttct gaacccggtg gtgatgcacc agactcttgt tacagatttt
    11761 taaccccatg aaaacacaat gcaaacacag ccatacccgg gttgtcccct tgcgttgtgt
    11821 ttctcaagtt aatagaatgc atcgatcgtt actatcatat cgatcgctat aaacgatcgt
    11881 ttgtggtaaa tgacctcctc cgtggttgtc atgaaaatgc gtctcaggaa ggttaaataa
    11941 tgcacgaaaa gaacatcgcc ctgctttgtg atgaagccga ccgacttttg caactgaaca
    12001 ttaatctgct ccggcaaatg gttgatgaac cggatgtgtt atctgacagt aagaacgaaa
    12061 acagactgct ttttgataaa cagaaagcac tgaaaagaat tgaggagctg gagggcgaac
    12121 aaatcaaaac cgcccgcagg gagatggtgc tggctgttgt cggcacgatg aaagcaggta
    12181 aatcaactac catcaacgcc attgtggggc aggaaattct gcctaaccgt aaccgcccca
    12241 tgacctctgt accgacgctc atccgccacg ttcccggaaa aactgagccg gtcctccatc
    12301 tggaacatat tcagcccgtc cgcaatttat taagcacact gcaacaaaaa ctcgacaccc
    12361 cggcaggaca gcaagtcgca cagaccctgc agcaaaccgg ggatacctgc gaactgctgg
    12421 atattctggc aaatgatggc tggctcaaaa atgaatacca cggggaagac gaaatcttta
    12481 ccggactggc atcgttaaac gatctggttc gccttgctgc ggcaatgggg agtgagtttc
    12541 cttttgatga atacgcagaa gtgcagaaac tgccggtgat cgacgtggaa ttcagccatc
    12601 tggtggggat ggatgcatgc cagggaacac tcacactgct ggataccccc ggccctaatg
    12661 aggccggaca accgcagatg gaagtgatga tgcgggatca actgcagaaa gcctctgcgg
    12721 tgctggcggt gatggattac acccagatga actcaaaagc ggatgaagac gtccgtaaag
    12781 agcttaatgc cattgctgac gtatcagccg gccgcctgtt tgtgctggtc aataaatttg
    12841 atgagaaaga ccgcaatggc gatggggcag atgccgtacg ccagaaagtt ccggcaatgc
    12901 tgaacagcga tgtgctgccc gcctcccgcg tttatccagg ctcatcacgt caggcgtacc
    12961 tggctaaccg tgcgcttcat gagttacgga aaaacagaac ccttcctgtt gatgaagcct
    13021 gggtcgatga ttttgtcagg gaagccttcg gtcgcatgaa aaaagaatac gtctgtaaag
    13081 acagtgaaat ggcaaccgaa ggggcgacag acctgtggga aggctcactt atcgatcaac
    13141 tgataacgga agtcatacag agctcccatt ccagagcggc ggcactggcg gttgattctg
    13201 ccgccgcaaa actgatgcag aatgcggaaa atgtcagcga atatctgtca ttacgccatc
    13261 aggggctaca gcaaagcatt cagtcactgc agtcgcatat caccagcctg cttgcggata
    13321 tccgggaaat cgcggactgt caggagcagg tgaccgctga tgtcagaatg gccatggagg
    13381 agatcgatgc cagaacccgg gaattactga cgggggtctg cacctcactg gaagaggagc
    13441 tgaatgacta tttcagaagc ggtaaacgca aagaacagca aatgctggag gaagaaaatt
    13501 cagcgcagcc ccgggagaga aacgcattcg ctttctttca cgatattttc ggaaccggaa
    13561 atcagcatga caggatgcgg gactttgatc cggacagccc cgaaataaaa ttcagtgatc
    13621 gcagggaagc ccttgaactg atgacgcaaa tcgaatcgac cgtgaccagc ctgcaccgtg
    13681 aggctgaagc acagttccgt cctgaactgg aaaaaatcgt caggggtatt gaaacaggtt
    13741 ttcgtggtac ggccctgtac gccacagaaa atattgccgg acgcatcaat gcccgtctgg
    13801 aggatgaagg tttcactgta aaaatcagtt ttccggcagt cagccagtta cagacccggc
    13861 tcgcagtaaa aacaaatctg agcgcgctta tggaggaaag aacggagaca gtaacccgtc
    13921 gctgtcgaca ggatggtgtc tggggtacgc tttgtcgatg ggcaaacacc agtgactggg
    13981 gctggaaaga gtacagtgtg gatgtgagcc gcagcgtgat caatatcaac aaggtcagag
    14041 aggaagtgat gtcactgacc cgggcatatt tcggggagct gcaggcatcc attgagcagg
    14101 atattaacca gcccgtccgc caggaaatcg atgccttttt ctgtacattc agggagaaag
    14161 ttgaacagct gcgtaacacg ctgattcaga gctctgaaga tcataaacgc gatcagcagg
    14221 cgcaggaacg gctgaccggg cgtcttcagg cgttaaatga aagggttcct gagctcatta
    14281 ctgacagtaa ggcgttaagg gaagaactgg agacaatgct gtgacttcac catttattca
    14341 acaaattgct gataaccggg tatgtcaggt actcacctgt cttcctgaaa aatttgtggt
    14401 tgatttcgca aacggtattg atgttgcaca ggagcatatc cgtacggccg gagagcgcac
    14461 gttcttccgg cggttaaaag aggggctgag cggcgaaggt actgcacggc agaatgccat
    14521 caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc tggctgacgg agctgacaac
    14581 gtcacttgcc accacaaact acgcgattac ccgggtaaac gacagggtca gttcactggt
    14641 cagtgatact gccaggctgg cgcattattc cgcagatacg cgggagcagt tactcaccct
    14701 ggccgatcag gttcaccaga aactgaatca tcttgaagaa aaactccacc gtgttgacct
    14761 ggttcagcgg gcgcagttac atcttgagca gatattctcc tggtggagcg ccgggcgata
    14821 tgcgtcattt tcccctgccg gacgttgtta tgtggcactt gaagagcttc gctggggcgc
    14881 gtttggtgat gtgatacgtc agggcgaaac aggccaggtt aaccagctac tggatattct
    14941 cagacataaa gcattaacgc agatggcaca ggagagtggc ggtagtgcaa ccgtgcgtct
    15001 gaacactctg gactggcttg gtggtcaggg ccgggagcag gcagataacg agtggcatga
    15061 cgcgattaac tggctggggg actggtgcag tgaagagcag catccggtga tctggtcaac
    15121 cacgcaggct gcagaacact taccggttcg catgccccgt ctctgctctg cagaacgcct
    15181 ctctgaaagt atggtcgatg aaatatttca gaagggggct gcatgagtat agaaataaaa
    15241 acagggctgg tgctgtccgg cggtggggcg gtgggcgctt atcaggcggg agtggttaag
    15301 gcactggcag agtgtggtac acagatcagc atggtttcag gggccagcat tggcgcattc
    15361 aatggtgcca ttatcgcggc ctctcccgat ctgtcagaag ctgccgtacg cctggaggcg
    15421 ctctgggatc atcttgggaa taatcaggtg ctgtcggtaa accgatcggt ttacttttca
    15481 ttgctgaaaa aattgttcca ggcaatgaac ctctgccaga tccccggacg tgcaggagca
    15541 ctgcttacga cacttcttcg ccatatatcg ataatcaacg gcttcgacaa tccgatgact
    15601 cagccgttgt tgtcagatgg gcccctgaca gcgttgatgg atcattatct tgatacagat
    15661 gctctggcag gcgggctacc gctgtatgtg tcgctgtacc ccacagaagg gggcatgcag
    15721 gatattattg actgcattcg tgctgaactg ggttccggaa ccacgaaaaa cgccgttttt
    15781 cagcatatcc agagtctgcc ccgcggacag cagaaagagg ctctgcttgc gtcagccgca
    15841 ctgcccctgt tgttccgtcc ccgtgaggtt caggggacaa tgttcggtga tggtggtatg
    15901 ggaggatggc gaaatatgca gggaaatacc cctgtaacgc ctctggtcga tgccggatgc
    15961 aatatggtga ttgtgtcgca tctgagtgac ggttctttat gggatcgcca ggcttttccg
    16021 gacaccacaa tccttgagat ccgtccccgg aaaaggctga aatatgcagg tgatggtggc
    16081 aacagtggcg gtctgctcag tttcacgtcg gcacataccg acgcctggcg tcagcagggc
    16141 tacgaggaca cgatgctgac gatggagcat atccggaaac cgctggcagc acgtcaggca
    16201 ctgtcccggt cagaaacggt gttgcagaaa agtctggaga taacggaaga ggcagatttg
    16261 gcactgagaa gcgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct
    16321 gtggtggtgt ttttcctgtc tggtgttgtt gaaaatattt tcagatatat attacctggg
    16381 tgagatttaa gattaacgca tttattaatg tcataaggag agttatcatg aaattcatcg
    16441 taaaagcact taggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttga
//
LOCUS       DADLDT020000093        16392 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000093 DADLDT020000000
VERSION     DADLDT020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.093"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="JQH22_002940"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="JQH22_002940"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFB2207195.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="JQH22_002941"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="JQH22_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFB2207196.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="JQH22_002942"
     CDS             2342..2557
                     /locus_tag="JQH22_002942"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207197.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="JQH22_002943"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="JQH22_002943"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFB2207198.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4452..5846
                     /gene="galP"
                     /locus_tag="JQH22_002944"
     CDS             4452..5846
                     /gene="galP"
                     /locus_tag="JQH22_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFB2207199.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5923..6420
                     /locus_tag="JQH22_002945"
     CDS             5923..6420
                     /locus_tag="JQH22_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFB2207200.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6515..7222
                     /gene="endA"
                     /locus_tag="JQH22_002946"
     CDS             6515..7222
                     /gene="endA"
                     /locus_tag="JQH22_002946"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFB2207201.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7302..8033
                     /gene="rsmE"
                     /locus_tag="JQH22_002947"
     CDS             7302..8033
                     /gene="rsmE"
                     /locus_tag="JQH22_002947"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFB2207202.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8046..8996
                     /gene="gshB"
                     /locus_tag="JQH22_002948"
     CDS             8046..8996
                     /gene="gshB"
                     /locus_tag="JQH22_002948"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFB2207203.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9105..9668
                     /locus_tag="JQH22_002949"
     CDS             9105..9668
                     /locus_tag="JQH22_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFB2207204.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9668..10084
                     /gene="ruvX"
                     /locus_tag="JQH22_002950"
     CDS             9668..10084
                     /gene="ruvX"
                     /locus_tag="JQH22_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFB2207205.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10260..11240)
                     /locus_tag="JQH22_002951"
     CDS             complement(10260..11240)
                     /locus_tag="JQH22_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFB2207206.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11258..11962
                     /gene="yggS"
                     /locus_tag="JQH22_002952"
     CDS             11258..11962
                     /gene="yggS"
                     /locus_tag="JQH22_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFB2207207.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11980..12546
                     /gene="yggT"
                     /locus_tag="JQH22_002953"
     CDS             11980..12546
                     /gene="yggT"
                     /locus_tag="JQH22_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFB2207208.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12543..12833
                     /locus_tag="JQH22_002954"
     CDS             12543..12833
                     /locus_tag="JQH22_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HFB2207209.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12841..13434
                     /locus_tag="JQH22_002955"
     CDS             12841..13434
                     /locus_tag="JQH22_002955"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFB2207210.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13427..14563
                     /gene="hemW"
                     /locus_tag="JQH22_002956"
     CDS             13427..14563
                     /gene="hemW"
                     /locus_tag="JQH22_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFB2207211.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14877..15840
                     /locus_tag="JQH22_002957"
                     /pseudo
     CDS             14877..15840
                     /locus_tag="JQH22_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15885..16388
                     /locus_tag="JQH22_002958"
     CDS             15885..16388
                     /locus_tag="JQH22_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFB2207212.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4062 a         4220 c         4127 g         3983 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctacgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagaccattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caagcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacgctac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgagtctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gccgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacgga ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccggcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca actgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct taattgcaga tgacagcgtt taatctatga tgatataact caattatttt
     4321 catgccctta aatcataact aagataaatg ttagtgtaag cgattacact gatgtgattt
     4381 gcttcacatc tttttacgtc gtactcacct atcttaattc acaataaaaa ataactatat
     4441 tggagggcat catgcctgac gctaaaaaac aggggcggtc aaacaaggca atgacgtttt
     4501 tcgtctgctt ccttgccgct ctggcgggat tactctttgg cctggatatc ggtgtaattg
     4561 ctggcgcact gccgtttatt gcagatgaat tccagataac ttcgcacacg caagaatggg
     4621 tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt gggcatcggc tggctctcct
     4681 ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat cctgtttgtt gccggttcac
     4741 tgttctctgc ggctgcgcca aacgttgaag tactgattct ttcgcgcgtt ctgctggggc
     4801 tggcggtggg tgtggcctct tataccgcac cactgtacct gtcagaaatt gcgccggaaa
     4861 aaattcgcgg cagtatgatc tcgatgtatc agttgatgat cactatcggg atcctcggtg
     4921 cttatctttc tgataccgcc ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga
     4981 ttatcatccc ggcaattttg ctgctgattg gtgtcttctt cctgccagac agcccacgtt
     5041 ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca
     5101 ccagcgcgga agcgaaacgc gaactggatg aaatccgtga aagtttgcag gttaaacaga
     5161 gtggctgggc gctgtttaaa gagaatagca acttccgccg cgcggtgttc cttggcgtac
     5221 tgttacaggt aatgcagcaa ttcaccggga tgaacgtcat catgtattac gcgccgaaaa
     5281 tcttcgaact ggcgggttat accaacacca ccgagcaaat gtgggggaca gtgattgtcg
     5341 gcctgaccaa cgtacttgcc acctttatcg caatcggcct tgttgaccgc tggggacgta
     5401 aaccaacgct aacgctgggc ttcctggtga tggctgctgg catgggcgta ctcggtacaa
     5461 tgatgcatat cggtatccac tctccgtcgg cgcagtattt cgccatcgcc atgctgctga
     5521 tgtttattgt cggttttgcc atgagtgccg gtccgctgat ttgggtactg tgctccgaaa
     5581 ttcagccgct gaaaggccgc gattttggca tcacctgctc caccgccacc aactggattg
     5641 ccaacatgat cgttggcgca acgttcctga ccatgctcaa cacgctgggt aacgccaaca
     5701 ccttctgggt gtatgcggct ctgaacgtac tgtttatcct gctgacattg tggctggtac
     5761 cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac
     5821 tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc ctcccgtcgc ggaggaagcc
     5881 gcctcttgca gtcatctttt cttcgctcta tcctctgccg ctatgaaaac atcccgtctc
     5941 cctatcgcca tccaacaggc cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac
     6001 ctgaagctag ggcgtaacta cccggagcca aaactctctt acacccagcg cggaacctcc
     6061 gccggaacag cctggctgga aagctatgaa atccgcctca atcccgtttt gctgttggaa
     6121 aacagtgaag cttttattga agaagtggta ccgcacgaac tggcacattt gctggtatgg
     6181 aaacatttcg gccgcgtagc gccacatggc aaagagtgga agtggatgat ggaaagcgtg
     6241 ctgggcgttc ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc
     6301 ttcccctacc gctgtaagtg ccaggaacat cagcttaccg tacgccgcca taatcgcgta
     6361 gttcgtggcg aggccatcta tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa
     6421 ccacctgaac tatcaggaac tttcctgatc tggctgattg cataccaaaa caactttcgc
     6481 tacgttgctg gctcgtttta acacggagta agtgatgtac cgttatttgt ctattgctgc
     6541 ggtggtactg agcgcagcat tttccggccc gacgttggcc gaaggtatca atagtttttc
     6601 tcaggcgaaa gccgcggcgg taaaagtcca cgctgatgcg cccggtacgt tttattgcgg
     6661 atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca
     6721 ggtgcgcaaa aatgaaaacc gcgccagccg cgtagagtgg gaacacgtcg ttcccgcctg
     6781 gcaattcggt caccagcgcc agtgctggca ggacggtgga cgtaaaaact gcgctaaaga
     6841 tccggtctat cgcaagatgg aaagcgatat gcataacctg cagccgtcag tcggcgaggt
     6901 gaatggcgat cgcggcaatt ttatgtacag ccagtggaat ggcggcgaag gccagtacgg
     6961 tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg
     7021 cggtgccatt gcgcgcacct acttctatat gcgcgaccaa tacaacctga cactctctcg
     7081 ccagcaaacg cagctgttca acgcatggaa caagatgtat ccggttaccg actgggagtg
     7141 cgagcgcgat gaacgcatcg cgaaggtgca gggcaatcat aacccgtatg tgcaacgcgc
     7201 ttgccaggcg cgaaagagct aacctacact agcgggattc tttttgttaa cccctacccc
     7261 acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc ccccgcattt
     7321 atcatcctga accactgacc agccattctc acatcgcgct ttgcgaagat gccgccaacc
     7381 atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt gacggtagca
     7441 accaggtctt tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc
     7501 tggaaggcca gatcgacgat cgcgaatctc cgctgcatat tcacctcggt caggtgatgt
     7561 cgcgtggtga aaaaatggaa tttactatcc agaaatcgat cgaactcggt gtaagcctca
     7621 ttacgccact tttttctgag cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga
     7681 agcttcagca gtggcagaag attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg
     7741 tgccggaaat ccgtccagcg atggatctgg aagcctggtg tgcagagcag gatgaaggac
     7801 tgaaactgaa tcttcacccg cgcgccagta acagcatcaa tacgttgcct ttaccggttg
     7861 aacgcgtccg cctgctgatt ggcccggaag gcggtttatc ggcagatgaa attgccatga
     7921 ctgcccgcta tcaatttact gatatcctgt tgggacctcg cgttttgcgt acagagacaa
     7981 ctgcgctcac cgccattacc gcgctacaag tacgatttgg cgatttgggc taacggagaa
     8041 gaataatgat caagctcggc atcgtgatgg accccatcgc aaacatcaac atcaagaaag
     8101 attccagttt cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg
     8161 agatgggcga tctgtatctg atcaatggcg aagcccgcgc ccatacccgc acgctgaacg
     8221 tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga acaggatctg ccgctggccg
     8281 atctcgatgt gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga
     8341 cctatattct ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc
     8401 tgcgcgactg taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc
     8461 tggttacgcg caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc
     8521 ttaagccgct ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa
     8581 acctcggcgt gattgccgaa accctgactg agcatggcac ttgctactgc atggcgcaaa
     8641 attacctgcc agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg
     8701 taccgtactg cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg
     8761 gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg
     8821 ggccgacgct gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc
     8881 tgactgaaat taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg
     8941 tgtcgatcac cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca
     9001 ccttagcgag aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac
     9061 tgggcgctgt tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc
     9121 actttcttat tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca
     9181 tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc
     9241 tcaaaattga agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa
     9301 tccgtctgga taaaccggtt atgctcggcg gcccactggc tgaagatcgc gggtttattt
     9361 tgcatactcc gccctccaat tttgcttcca gcattcgcat ttcagacaac acggtgatga
     9421 ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg
     9481 tggctctggg ctatgcctcc tgggaaaaag gtcaactgga acaagaaatt ctcgataacg
     9541 cgtggctaac ggccccggcg gatctgaata ttctgttcaa aacgccgatt gccggccgct
     9601 ggcgcgaggc ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcaggac
     9661 acgcctgatg agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc
     9721 ggtcggccaa cgcattaccg gcaccgctcg ccctttgcct gcaattaaag cgcaggacgg
     9781 tacgccggac tggaacctca tcgaacgttt actgaaagag tggcagcctg acgaaatcat
     9841 cgtcggtttg ccgctgaata tggacggcac cgaacagccg ttaaccgcca gagcgcgcaa
     9901 atttgccaac cgtattcatg gtcgtttcgg tgttgaagta aagctccatg acgagcgtct
     9961 tagcactgtg gaagcccgtt ccggtctttt tgaacagggc ggctatcggg cgctcaacaa
    10021 aggcaaagtt gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata
    10081 ttaaggcgat ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg
    10141 aacgccttat ccagcacaca tctggcagcg gctgtaggtc tgataagacg cgttagcgtc
    10201 gcatcagaca tttattgcct ttataggccc tataaacttg cgcatcgggc atggcaacgt
    10261 cacaaacgcc cttcccccac ccgctgctga taactctgct gaaacgttaa catccccacc
    10321 tgctgcccgg tttgaataac atgcggtaac tggtgggttt tcccttcgcg aatcaaattc
    10381 cccaccgcgg gagtgttaat cagtaattca aacagcgcca cgcgtccttc ctgtttatcc
    10441 acttccagct tttgtgacag cactgcccgt aaactacccg caagttgatt acgtacggga
    10501 tctttttcct gcgccggaaa tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgt
    10561 gtatgtaagg ttgccagcac cagatgcccg gtttctgccg ccgtcagcgc cagacggatt
    10621 gtctcactgt cacgtagctc tccgagcaaa atcacatcag gatcttcccg caatgcagcc
    10681 cgcaaccccg atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat
    10741 cgctggctgg catagagata ttccacagga tcttccagcg tcagaatatg cgcatcggca
    10801 tgttgattaa gatagccaac catcgccgcc agcgtggtag atttgccact ccccgtagcc
    10861 cccgtcacca gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cggtggtgca
    10921 ccaagctgtt cgagctgtgg acagtgcgaa ggtaacaacc gtaatgccag agaaatacct
    10981 tgccgttgcg cgaacgcact gccacgcaac cgctggtttt ccgccagcga cacggcaaaa
    11041 tccagctggc cattttccag caatattgcc cgctgatcgt catccagcca ctcccgcagt
    11101 agctcttcga cgtccggcgc atcaaacggc gcagcttcca ttctgccacg aatgcgccat
    11161 cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc
    11221 acaatttctt ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct
    11281 ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga
    11341 aattacgctg cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga
    11401 tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca
    11461 ctttcaggaa ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa
    11521 taaaagccgc ctagtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat
    11581 tgctacccgc ctcaacgagc agcgcccggc agaacttccc cctcttaacg tactgattca
    11641 aattaatatt agtgatgaaa acagtaagtc cgggattcaa ctggcagaac tggacgagct
    11701 ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc
    11761 gcctgagtca gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc
    11821 cggactgaaa acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat
    11881 ggaagccgcc attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc
    11941 gcgtgattac tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg
    12001 ctttcaacgg tcattgagct gtatacaatg gtgctgttat tacgcatctg gatgcagtgg
    12061 gctcattgtg atttttacaa ccccttctca cagtttgtag tgaaggtagc gcagccaatt
    12121 atcgggccac tgcgccgcgt tattccggca atgggtccca ttgacagcgc ctcgctgctg
    12181 gttgcctata ttctcagttt tatcaaagcc attgtgctgt ttgaagtggt gaccttcctg
    12241 ccaatcatct ggattgccgg tttactgatt ctgctgaaaa ccatcggctt gctgattttc
    12301 tgggtcctgc tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa
    12361 tacgtgctga ttcagctggc cgatccgctg ctgcgcccga ttcgccgctt gctaccggca
    12421 atgggtggga ttgatttctc gccgatgatc ctcgttctgc tgctgtatgc catcaatatg
    12481 ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc tgccggggct gtggatggcg
    12541 ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt acagctctat attcagccga
    12601 aagccagccg tgattctatt gtcggtttac atggcgacga agttaaagtc gccattaccg
    12661 cgccgccggt tgacggccag gccaacagtc atctggtgaa gtttctcggt aagcaattcc
    12721 gggttgccaa aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta
    12781 aaatcattaa tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct
    12841 atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg
    12901 cttagcgact ttggtcttga tatcgtggcc caaacagacc tcggcgttga ttccgctgaa
    12961 gaaaccggcc taacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg
    13021 accggtttac cggcaattgc cgacgactct ggtctggcgg tagatgcgct gggtggcgcg
    13081 ccgggtattt actccgcgcg ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa
    13141 aaactgttgg aaacactgaa agacgtaccg gacgaccaac gtcaggcgcg tttccactgc
    13201 gtgctggtat atctgcgtca cgcggaagat ccgactccgc tggtatgcca cggcagctgg
    13261 ccgggcgtga ttactcgcga accagcgggc actggtggct ttggttatga tccaatcttc
    13321 ttcgtacctt ccgaagggaa aaccgccgcc gaactgaccc gcgaagaaaa gagcgccatt
    13381 tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc
    13441 gccgctgagt ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt
    13501 caactctcac gcgttgaaag gagaagtgcc gcacgacgat tatgttcagc atctgctttg
    13561 cgatctggac aatgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg
    13621 cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa acgctgctag acggcgtgcg
    13681 tgcgcgtttg ccgctggcag cggatgcaga aattactatg gaagcgaacc caggcacggt
    13741 agaagcggat cgctttgtcg attatcagcg tgctggtgta aaccgcatct ctattggtgt
    13801 gcagagtttt agcgaagaaa agctgaaacg acttgggcgt attcatggcc cgcaagaagc
    13861 gaaacgagcg gcaaagctgg cgagcggttt agggttacgt agctttaacc ttgatttgat
    13921 gcatggacta ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggccattga
    13981 actgaatccg ccgcatcttt cctggtatca actgactatc gaacctaata cgctgtttgg
    14041 ctcgcgaccg ccggtgctgc cggacgatga cgcgctgtgg gatattttcg aacaggggca
    14101 tcagttatta accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaacccgg
    14161 ttatcagtgc cagcacaatc tcaactactg gcgctttggt gactacatcg gtattggctg
    14221 cggcgcgcac ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg
    14281 tcatcctcgt ggttttatgc aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc
    14341 agataagccg tttgagttct ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt
    14401 ggagtttatt gcgtataccg ggctttgcga agatgtgatt cgcccacagt tagacgaggc
    14461 gattgcccag ggttatctca ccgaatgtgc ggattactgg cagataacgg aacatggtaa
    14521 attattctta aactcacttc ttgaattatt cctcgctgag taatcattaa tgcgcctgcg
    14581 ggcgcattaa tcacttcttt tatccccccg ttaattaacc tcggcagaaa tatatttctt
    14641 actgaataaa tatcgacgac atttattata tatatttagt gaatatttac taatcattca
    14701 ttgattataa atatgtacaa cctggcgcac aaatttaata tttaatttct gactttattt
    14761 caagttgtga taaacataga caagttgtca gacaggttaa ccaatttgtc gcacaaatca
    14821 ctgataagca caattagata tcaaccaatt tccatccttc aacagggagt ttttttatga
    14881 aaatgaggtc atttacccgc tcattagtct gcgcatcctt gctggctctg gttagcactg
    14941 gcgtaaacgc cgcagaaaaa gtggcgttaa agttagcaca caacctggaa agaagtcatg
    15001 ttgtacatca gagttttgaa gagctggcga aagaggtaaa acagctgtca aaaggtaata
    15061 tggtgatccg tatttatccc agcagccaga tgggtaatgc gcgagaaact atggaattgt
    15121 tgcaaaatgg cgcactggat atgaccaaag gttccgccag tgatctggag tcttttgata
    15181 atatttatgc tatctataat ttgccgttct tattaagttg tttttggtga agttggtaaa
    15241 gaaattatgg actcaacaaa agacaaaggc ttttttgctt tatcagctta tgtagcgggt
    15301 actcgcagtt tctatgcgaa aaaaccgatt acaaagccag aagatcttaa aggactgaaa
    15361 attcgtgttc agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc
    15421 actccaattt catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca
    15481 gaaaataacg tgccttcctg gatgcagact cgacatattg aaattgccaa agttttttct
    15541 gaagatgaac acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa
    15601 ttaaccccag aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa
    15661 caaaaattgt gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt
    15721 gaaattgtaa aagtggataa agcgccattc cgtaaagccg tgcaacctct gtttgacgac
    15781 ttcaaaaaag atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa
    15841 tattccgggc cagcctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc
    15901 tgaaacttgc ggtagaccgc gtcattgccg cgttttctgt cgccgtaatg cttgctctgg
    15961 ttgtatgcgt agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa
    16021 ccgatgagct ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg
    16081 tcggcgcgca gcgacatctc tctattgatt tatttgctct cgccctgaat aagcgcaaac
    16141 aattacttct tagtatagtc attaacgttc tcattttagg ttttgccggt tcggtcattg
    16201 tgactggtgg cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcta
    16261 tgcaaatacc gatgggatac gtatatatca ttctgccatt aagtggagtg gtaatgatgt
    16321 tttatgcctt gtgttttatt aatcaaagca ttcaacaact gaaacaacct gcgcaggagg
    16381 ccagttaatg ga
//
LOCUS       DADLDT020000094        15543 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000094 DADLDT020000000
VERSION     DADLDT020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.094"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            216..410
                     /gene="bfd"
                     /locus_tag="JQH22_002959"
     CDS             216..410
                     /gene="bfd"
                     /locus_tag="JQH22_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFB2207213.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            483..959
                     /gene="bfr"
                     /locus_tag="JQH22_002960"
     CDS             483..959
                     /gene="bfr"
                     /locus_tag="JQH22_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFB2207214.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(956..1423)
                     /locus_tag="JQH22_002961"
     CDS             complement(956..1423)
                     /locus_tag="JQH22_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFB2207215.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1804..2115
                     /gene="rpsJ"
                     /locus_tag="JQH22_002962"
     CDS             1804..2115
                     /gene="rpsJ"
                     /locus_tag="JQH22_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFB2207216.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2148..2777
                     /gene="rplC"
                     /locus_tag="JQH22_002963"
     CDS             2148..2777
                     /gene="rplC"
                     /locus_tag="JQH22_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFB2207217.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2788..3393
                     /gene="rplD"
                     /locus_tag="JQH22_002964"
     CDS             2788..3393
                     /gene="rplD"
                     /locus_tag="JQH22_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFB2207218.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3390..3692
                     /gene="rplW"
                     /locus_tag="JQH22_002965"
     CDS             3390..3692
                     /gene="rplW"
                     /locus_tag="JQH22_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFB2207219.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3710..4531
                     /gene="rplB"
                     /locus_tag="JQH22_002966"
     CDS             3710..4531
                     /gene="rplB"
                     /locus_tag="JQH22_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFB2207220.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4548..4826
                     /gene="rpsS"
                     /locus_tag="JQH22_002967"
     CDS             4548..4826
                     /gene="rpsS"
                     /locus_tag="JQH22_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFB2207221.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4841..5173
                     /gene="rplV"
                     /locus_tag="JQH22_002968"
     CDS             4841..5173
                     /gene="rplV"
                     /locus_tag="JQH22_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFB2207222.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5191..5892
                     /gene="rpsC"
                     /locus_tag="JQH22_002969"
     CDS             5191..5892
                     /gene="rpsC"
                     /locus_tag="JQH22_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFB2207223.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5905..6315
                     /gene="rplP"
                     /locus_tag="JQH22_002970"
     CDS             5905..6315
                     /gene="rplP"
                     /locus_tag="JQH22_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFB2207224.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6315..6506
                     /gene="rpmC"
                     /locus_tag="JQH22_002971"
     CDS             6315..6506
                     /gene="rpmC"
                     /locus_tag="JQH22_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFB2207225.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6506..6760
                     /gene="rpsQ"
                     /locus_tag="JQH22_002972"
     CDS             6506..6760
                     /gene="rpsQ"
                     /locus_tag="JQH22_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFB2207226.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6925..7296
                     /gene="rplN"
                     /locus_tag="JQH22_002973"
     CDS             6925..7296
                     /gene="rplN"
                     /locus_tag="JQH22_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFB2207227.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7307..7621
                     /gene="rplX"
                     /locus_tag="JQH22_002974"
     CDS             7307..7621
                     /gene="rplX"
                     /locus_tag="JQH22_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFB2207228.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7636..8175
                     /gene="rplE"
                     /locus_tag="JQH22_002975"
     CDS             7636..8175
                     /gene="rplE"
                     /locus_tag="JQH22_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFB2207229.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8190..8495
                     /gene="rpsN"
                     /locus_tag="JQH22_002976"
     CDS             8190..8495
                     /gene="rpsN"
                     /locus_tag="JQH22_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFB2207230.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8529..8921
                     /gene="rpsH"
                     /locus_tag="JQH22_002977"
     CDS             8529..8921
                     /gene="rpsH"
                     /locus_tag="JQH22_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFB2207231.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8934..9467
                     /gene="rplF"
                     /locus_tag="JQH22_002978"
     CDS             8934..9467
                     /gene="rplF"
                     /locus_tag="JQH22_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFB2207232.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9477..9830
                     /gene="rplR"
                     /locus_tag="JQH22_002979"
     CDS             9477..9830
                     /gene="rplR"
                     /locus_tag="JQH22_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFB2207233.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9845..10348
                     /gene="rpsE"
                     /locus_tag="JQH22_002980"
     CDS             9845..10348
                     /gene="rpsE"
                     /locus_tag="JQH22_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFB2207234.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10352..10531
                     /gene="rpmD"
                     /locus_tag="JQH22_002981"
     CDS             10352..10531
                     /gene="rpmD"
                     /locus_tag="JQH22_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFB2207235.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10535..10969
                     /gene="rplO"
                     /locus_tag="JQH22_002982"
     CDS             10535..10969
                     /gene="rplO"
                     /locus_tag="JQH22_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFB2207236.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10977..12308
                     /gene="secY"
                     /locus_tag="JQH22_002983"
     CDS             10977..12308
                     /gene="secY"
                     /locus_tag="JQH22_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFB2207237.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12340..12456
                     /gene="rpmJ"
                     /locus_tag="JQH22_002984"
     CDS             12340..12456
                     /gene="rpmJ"
                     /locus_tag="JQH22_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFB2207238.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12603..12959
                     /gene="rpsM"
                     /locus_tag="JQH22_002985"
     CDS             12603..12959
                     /gene="rpsM"
                     /locus_tag="JQH22_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFB2207239.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12976..13365
                     /gene="rpsK"
                     /locus_tag="JQH22_002986"
     CDS             12976..13365
                     /gene="rpsK"
                     /locus_tag="JQH22_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFB2207240.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13399..14019
                     /gene="rpsD"
                     /locus_tag="JQH22_002987"
     CDS             13399..14019
                     /gene="rpsD"
                     /locus_tag="JQH22_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFB2207241.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14045..15034
                     /gene="rpoA"
                     /locus_tag="JQH22_002988"
     CDS             14045..15034
                     /gene="rpoA"
                     /locus_tag="JQH22_002988"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFB2207242.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15075..15458
                     /gene="rplQ"
                     /locus_tag="JQH22_002989"
     CDS             15075..15458
                     /gene="rplQ"
                     /locus_tag="JQH22_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFB2207243.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3648 c         4138 g         3749 t
ORIGIN      
        1 ggcgcgggcg ttgttgctaa agttctgggc taattacacg ttaattagtt ttgaattgaa
       61 aagggcgctt cggcgccctt tttgctgccc gtaatttgag ctcgaaatat ttagtcgtaa
      121 ttttgctcgc tgaactatgg cgcaaataaa actgtactat tgtaatcata accattctca
      181 tttacacttt gtgcagaatt tgaacgggag caatcatgta cgtttgtttg tgtaatggtg
      241 taagtgacaa aaaaattcgc caggcagtcc gtcagtttca tccgcaatct ttccaacaat
      301 tgcgtaagtt tattcctgtg ggaaatcaat gcggtaagtg cattcgcgcc gcgcgcgaag
      361 tgatgcagga cgagttgatg cagatgccag aatttaaaga gattgcctga agcccacgct
      421 tattttttga catcctgcta gccccatcta cgctctaata gtggaagcgg agggactata
      481 aaatgaaagg tgatgttaaa atcataaatt atctcaataa actattggga aatgagcttg
      541 tcgcaatcaa tcagtatttt ctccatgccc ggatgtttaa aaactggggc ctgatgcgtc
      601 tcaatgacgt cgaataccat gaatccatcg atgagatgaa acacgctgat aaatatatcg
      661 agcgtatttt gtttttagaa ggaatcccca acttacagga tttaggcaaa ctggggattg
      721 gtgaagatgt cgaggagatg ctgcaatctg acctgcgatt agaactggaa ggcgcgaagg
      781 atctgcgtga ggctatcgca tatgccgaca gcgttcatga ctatgttagc cgcgatatga
      841 tgatcgagat ccttgctgat gaagaaggtc atatcgactg gctcgaaact gaactggatc
      901 tcatcggtaa aatcggccta caaaattacc tgcaatcaca aattaaagtt aaagattaac
      961 ggcctatcag caaactatcc cagcctataa cgaaacccgc agccgccaga aatggcccaa
     1021 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
     1081 cgcaagcgaa tgaggcagca aggaaaacca atcgtggtaa gaaggcccat gaatgccagg
     1141 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
     1201 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgcc ccccataacg
     1261 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
     1321 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
     1381 ataaacaagc gtacaaaatt agaaagggaa gggtagtgtc cataaaacat cctctgtaat
     1441 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagagc gctgatttgt
     1501 taacctgatt ccggtgaaaa acgctatctt cacaacctta caaatttcgt ggaatgcagc
     1561 tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt gcgtcaggct cagatttgcg
     1621 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1681 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1741 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1801 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1861 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1921 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1981 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     2041 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     2101 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     2161 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     2221 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     2281 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     2341 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     2401 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgacgtta
     2461 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     2521 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     2581 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     2641 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2701 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2761 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2821 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2881 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2941 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     3001 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     3061 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     3121 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     3181 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     3241 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc agaaggttga cgtacgcgat
     3301 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     3361 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     3421 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     3481 tactcaaagt tgcgaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     3541 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     3601 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3661 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3721 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3781 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3841 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3901 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3961 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     4021 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     4081 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     4141 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     4201 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     4261 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     4321 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     4381 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     4441 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     4501 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     4561 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     4621 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4681 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtaaccgacg
     4741 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4801 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta ttgctaaaca
     4861 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4921 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtacaggt
     4981 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     5041 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     5101 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     5161 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     5221 cgcttgggta ttgtaaaacc atggaactct acttggtttg cgaacaccaa agaattcgct
     5281 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     5341 tccgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     5401 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     5461 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     5521 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     5581 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     5641 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5701 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5761 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5821 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5881 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5941 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     6001 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     6061 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     6121 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     6181 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     6241 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     6301 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcattgaa gagctgaaca
     6361 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     6421 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     6481 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     6541 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     6601 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6661 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6721 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6781 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6841 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6901 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6961 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     7021 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     7081 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     7141 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     7201 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     7261 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     7321 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     7381 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     7441 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     7501 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     7561 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     7621 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7681 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7741 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7801 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7861 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7921 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtatccg tgacttccgt
     7981 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     8041 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     8101 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     8161 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     8221 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     8281 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     8341 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc
     8401 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     8461 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     8521 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     8581 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     8641 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8701 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8761 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8821 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8881 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8941 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     9001 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     9061 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     9121 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     9181 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     9241 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     9301 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc
     9361 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttacaaaggc aagggtgttc
     9421 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     9481 ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     9541 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     9601 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9661 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9721 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9781 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9841 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9901 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9961 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
    10021 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
    10081 cactctacaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
    10141 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
    10201 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
    10261 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
    10321 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
    10381 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggct tgggtctgcg
    10441 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
    10501 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
    10561 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
    10621 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10681 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10741 taaagcagcg attacagccg aagttcgtct atctgacctg gctaaagtag aaggcggtgt
    10801 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10861 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10921 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10981 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    11041 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    11101 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    11161 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    11221 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    11281 tggcagaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt
    11341 acggtactct ggtgctggca atattccagt cgatcggtat tgctaccggt ctgccgaata
    11401 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    11461 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatc actgaacgag
    11521 gtatcggcaa cggtatttca atcattatct tcgccggtat tgtcgcggga ctcccgccag
    11581 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    11641 tggttgcagt attagtattt gcagtgacgt tctttgttgt gtttgttgag cgtggtcaac
    11701 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga
    11761 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11821 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11881 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11941 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    12001 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    12061 cggcgaagta tatcgataaa gtaatgaccc gcctgacctt ggttggtgcg ctgtatatta
    12121 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    12181 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    12241 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    12301 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    12361 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    12421 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    12481 agttgggttg agctggctag attagccagc caatcttttg tatgtctgta cgtttccatt
    12541 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    12601 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12661 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12721 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12781 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12841 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12901 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12961 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    13021 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    13081 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    13141 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    13201 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    13261 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    13321 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    13381 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    13441 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    13501 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    13561 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    13621 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13681 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13741 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13801 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13861 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacttggct ggaagttgat
    13921 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13981 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    14041 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    14101 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    14161 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    14221 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    14281 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    14341 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    14401 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    14461 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    14521 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    14581 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    14641 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14701 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14761 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14821 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14881 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14941 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcttgga
    15001 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    15061 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    15121 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    15181 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    15241 agactgatag cgttgctaat cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg
    15301 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    15361 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    15421 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    15481 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    15541 ttt
//
LOCUS       DADLDT020000095        15408 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000095 DADLDT020000000
VERSION     DADLDT020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.095"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1173)
                     /locus_tag="JQH22_002990"
     CDS             complement(<1..1173)
                     /locus_tag="JQH22_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFB2207244.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="JQH22_002991"
     tRNA            complement(1372..1456)
                     /locus_tag="JQH22_002991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="JQH22_002992"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="JQH22_002992"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFB2207245.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="JQH22_002993"
     CDS             2800..4302
                     /locus_tag="JQH22_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HFB2207246.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="JQH22_002994"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="JQH22_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFB2207247.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="JQH22_002995"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="JQH22_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFB2207248.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="JQH22_002996"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="JQH22_002996"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFB2207249.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="JQH22_002997"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="JQH22_002997"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFB2207250.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="JQH22_002998"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="JQH22_002998"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFB2207251.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="JQH22_002999"
     CDS             complement(11867..13063)
                     /locus_tag="JQH22_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HFB2207252.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="JQH22_003000"
     CDS             13256..13759
                     /locus_tag="JQH22_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2207253.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="JQH22_003001"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="JQH22_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFB2207254.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="JQH22_003002"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="JQH22_003002"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFB2207255.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3933 c         3966 g         3534 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttca agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DADLDT020000096        15356 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000096 DADLDT020000000
VERSION     DADLDT020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.096"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQH22_003003"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQH22_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HFB2207256.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="JQH22_003004"
     CDS             complement(2138..2584)
                     /locus_tag="JQH22_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFB2207257.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="JQH22_003005"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="JQH22_003005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFB2207258.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQH22_003006"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQH22_003006"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFB2207259.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="JQH22_003007"
     CDS             complement(5347..6009)
                     /locus_tag="JQH22_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFB2207260.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="JQH22_003008"
     CDS             6182..6595
                     /locus_tag="JQH22_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFB2207261.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQH22_003009"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQH22_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFB2207262.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQH22_003010"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQH22_003010"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFB2207263.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="JQH22_003011"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="JQH22_003011"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFB2207264.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQH22_003012"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQH22_003012"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFB2207265.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQH22_003013"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQH22_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFB2207266.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="JQH22_003014"
     tRNA            complement(9861..9948)
                     /locus_tag="JQH22_003014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="JQH22_003015"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="JQH22_003015"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFB2207267.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="JQH22_003016"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="JQH22_003016"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFB2207268.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="JQH22_003017"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="JQH22_003017"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFB2207269.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="JQH22_003018"
     CDS             14005..14592
                     /locus_tag="JQH22_003018"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFB2207270.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="JQH22_003019"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="JQH22_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFB2207271.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..>15356
                     /locus_tag="JQH22_003020"
     CDS             14984..>15356
                     /locus_tag="JQH22_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFB2207272.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRR"
BASE COUNT         3824 a         3913 c         4125 g         3494 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcg
//
LOCUS       DADLDT020000097        15342 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000097 DADLDT020000000
VERSION     DADLDT020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.097"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQH22_003021"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQH22_003021"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFB2207273.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQH22_003022"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQH22_003022"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFB2207274.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQH22_003023"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQH22_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFB2207275.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="JQH22_003024"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="JQH22_003024"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFB2207276.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="JQH22_003025"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="JQH22_003025"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFB2207277.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="JQH22_003026"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="JQH22_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFB2207278.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="JQH22_003027"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="JQH22_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFB2207279.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="JQH22_003028"
     CDS             complement(8064..9206)
                     /locus_tag="JQH22_003028"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFB2207280.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="JQH22_003029"
     CDS             complement(9215..10228)
                     /locus_tag="JQH22_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HFB2207281.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQH22_003030"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQH22_003030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFB2207282.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQH22_003031"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQH22_003031"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFB2207283.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQH22_003032"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQH22_003032"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFB2207284.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="JQH22_003033"
     CDS             complement(12826..13950)
                     /locus_tag="JQH22_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFB2207285.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQH22_003034"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQH22_003034"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFB2207286.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4377 a         3445 c         3146 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcagca agaattggcg ggtaaattgc tatttaagtc aa
//
LOCUS       DADLDT020000098        15254 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000098 DADLDT020000000
VERSION     DADLDT020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15254
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.098"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..73)
                     /locus_tag="JQH22_003035"
     CDS             complement(<1..73)
                     /locus_tag="JQH22_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFB2207287.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="JQH22_003036"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="JQH22_003036"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFB2207288.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="JQH22_003037"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="JQH22_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFB2207289.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="JQH22_003038"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="JQH22_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFB2207290.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="JQH22_003039"
     CDS             2703..2939
                     /locus_tag="JQH22_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFB2207291.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="JQH22_003040"
     CDS             2942..3799
                     /locus_tag="JQH22_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2207292.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5796
                     /locus_tag="JQH22_003041"
                     /pseudo
     CDS             3799..5796
                     /locus_tag="JQH22_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="JQH22_003042"
     CDS             complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="JQH22_003042"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HFB2207293.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6399..7295)
                     /locus_tag="JQH22_003043"
     CDS             complement(6399..7295)
                     /locus_tag="JQH22_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFB2207294.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7344..7583)
                     /locus_tag="JQH22_003044"
     CDS             complement(7344..7583)
                     /locus_tag="JQH22_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFB2207295.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7686..8285
                     /gene="nemR"
                     /locus_tag="JQH22_003045"
     CDS             7686..8285
                     /gene="nemR"
                     /locus_tag="JQH22_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFB2207296.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8322..9419
                     /gene="nemA"
                     /locus_tag="JQH22_003046"
     CDS             8322..9419
                     /gene="nemA"
                     /locus_tag="JQH22_003046"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFB2207297.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9500..9907
                     /gene="gloA"
                     /locus_tag="JQH22_003047"
     CDS             9500..9907
                     /gene="gloA"
                     /locus_tag="JQH22_003047"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFB2207298.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10010..10657
                     /gene="rnt"
                     /locus_tag="JQH22_003048"
     CDS             10010..10657
                     /gene="rnt"
                     /locus_tag="JQH22_003048"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFB2207299.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10750..15208
                     /locus_tag="JQH22_003049"
                     /pseudo
     CDS             10750..15208
                     /locus_tag="JQH22_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT         3502 a         4029 c         4015 g         3708 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa
     5101 tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc
     5161 aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact
     5221 aatcccccgg tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc
     5281 gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc
     5341 cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca
     5401 ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc
     5461 caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct
     5521 catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg
     5581 gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca
     5641 ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa
     5701 cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt
     5761 gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata
     5821 gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg
     5881 gaccatcagc gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc
     5941 atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt
     6001 accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc
     6061 cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg
     6121 gaagccatgt tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc
     6181 ggtttcagta atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt
     6241 catctcgact ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc
     6301 cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca
     6361 gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt
     6421 gcagctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca
     6481 gctgcccgta cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa
     6541 cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc
     6601 acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca
     6661 aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc
     6721 agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc
     6781 agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc
     6841 actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac
     6901 gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac
     6961 tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct
     7021 tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga
     7081 ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca
     7141 gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg
     7201 cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc
     7261 gggccttccg gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa
     7321 gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac
     7381 gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct
     7441 tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag
     7501 ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga
     7561 aagaactcta attgctccgc cacctcgccc tcctcagata agattattac cattattgaa
     7621 gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct actacactcc
     7681 aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc
     7741 aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg
     7801 aagtgccgaa agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca
     7861 tgcttgagcg tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg
     7921 aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc
     7981 aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt
     8041 cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc
     8101 aggctctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac
     8161 aggcacaagt gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca
     8221 gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg
     8281 tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt
     8341 attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga
     8401 cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     8461 gccaacgtgc cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa
     8521 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     8581 tcaccgctgg cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac
     8641 gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta
     8701 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat
     8761 ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg
     8821 ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt
     8881 atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca
     8941 gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg
     9001 gtgccgatcg cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg
     9061 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     9121 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     9181 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     9241 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     9301 ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc
     9361 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     9421 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     9481 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     9541 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     9601 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     9661 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     9721 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     9781 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     9841 agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg
     9901 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     9961 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
    10021 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
    10081 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
    10141 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
    10201 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
    10261 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
    10321 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
    10381 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
    10441 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
    10501 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
    10561 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
    10621 gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg
    10681 caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga
    10741 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
    10801 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
    10861 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
    10921 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
    10981 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
    11041 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
    11101 cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt
    11161 gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga
    11221 attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat
    11281 cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta
    11341 ccggtcgcca atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat
    11401 gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg
    11461 cgccatcgct cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca
    11521 cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg
    11581 gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt
    11641 attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg
    11701 ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat
    11761 atgggcgcgg tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta
    11821 caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc
    11881 cgtacccggc gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg
    11941 ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc
    12001 gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca
    12061 ccgtggaaag atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc
    12121 tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg
    12181 atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg
    12241 gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg
    12301 gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc
    12361 ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct
    12421 ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa
    12481 ttaggcgaga tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg
    12541 actattcccc cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac
    12601 gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc
    12661 cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat
    12721 gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct
    12781 gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc
    12841 gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta
    12901 ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg
    12961 ccgggccgca cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag
    13021 ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa
    13081 tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt
    13141 ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta
    13201 ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg
    13261 gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg
    13321 ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct
    13381 cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca
    13441 atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat
    13501 ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt
    13561 cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag
    13621 tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc
    13681 tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt
    13741 attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg
    13801 caacaaaaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa
    13861 gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa
    13921 cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcta
    13981 ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt
    14041 ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca
    14101 gaaatgctcg atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct
    14161 gggtgaagat gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac
    14221 tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct
    14281 ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt
    14341 cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt
    14401 cattaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg
    14461 cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt
    14521 ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt
    14581 ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    14641 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    14701 gcaaacactt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    14761 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    14821 gacacaagcc acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc
    14881 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg
    14941 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    15001 cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca
    15061 ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa
    15121 tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    15181 ttcgccacaa gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt
    15241 ccctgctacg ttca
//
LOCUS       DADLDT020000099        15098 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000099 DADLDT020000000
VERSION     DADLDT020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.099"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="JQH22_003050"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="JQH22_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFB2207300.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="JQH22_003051"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="JQH22_003051"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFB2207301.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="JQH22_003052"
     CDS             1531..2478
                     /locus_tag="JQH22_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFB2207302.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="JQH22_003053"
     CDS             2548..3354
                     /locus_tag="JQH22_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFB2207303.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="JQH22_003054"
                     /pseudo
     CDS             3364..3771
                     /locus_tag="JQH22_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3771..4265
                     /locus_tag="JQH22_003055"
     CDS             3771..4265
                     /locus_tag="JQH22_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFB2207304.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="JQH22_003056"
     CDS             4331..5128
                     /locus_tag="JQH22_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFB2207305.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="JQH22_003057"
     CDS             5139..5927
                     /locus_tag="JQH22_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFB2207306.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="JQH22_003058"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="JQH22_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFB2207307.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="JQH22_003059"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="JQH22_003059"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFB2207308.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="JQH22_003060"
     CDS             complement(8370..10001)
                     /locus_tag="JQH22_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFB2207309.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="JQH22_003061"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="JQH22_003061"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFB2207310.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="JQH22_003062"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="JQH22_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFB2207311.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3936 g         3589 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatagcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggagaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtagaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca tggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct agtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gtacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattaa gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 ctcaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg tcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttca
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaa
//
LOCUS       DADLDT020000100        15049 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000100 DADLDT020000000
VERSION     DADLDT020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.100"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..176)
                     /locus_tag="JQH22_003063"
     CDS             complement(<1..176)
                     /locus_tag="JQH22_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207312.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="JQH22_003064"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="JQH22_003064"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFB2207313.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="JQH22_003065"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="JQH22_003065"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HFB2207314.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="JQH22_003066"
     CDS             complement(2012..2884)
                     /locus_tag="JQH22_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFB2207315.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="JQH22_003067"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="JQH22_003067"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFB2207316.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="JQH22_003068"
     CDS             complement(3576..4361)
                     /locus_tag="JQH22_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HFB2207317.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="JQH22_003069"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="JQH22_003069"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFB2207318.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="JQH22_003070"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="JQH22_003070"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFB2207319.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="JQH22_003071"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="JQH22_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFB2207320.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="JQH22_003072"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="JQH22_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFB2207321.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="JQH22_003073"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="JQH22_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="JQH22_003074"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="JQH22_003074"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFB2207322.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="JQH22_003075"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="JQH22_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="JQH22_003076"
     CDS             13253..14656
                     /locus_tag="JQH22_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207323.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15049
                     /gene="ompL"
                     /locus_tag="JQH22_003077"
     CDS             14724..>15049
                     /gene="ompL"
                     /locus_tag="JQH22_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFB2207324.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3699 a         3873 c         3744 g         3733 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaattaa tgataagagt atcagttcgg gtggtgcagt gtatctgga
//
LOCUS       DADLDT020000101        15030 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000101 DADLDT020000000
VERSION     DADLDT020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.101"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..236
                     /locus_tag="JQH22_003078"
     CDS             <1..236
                     /locus_tag="JQH22_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFB2207325.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="JQH22_003079"
     CDS             246..884
                     /locus_tag="JQH22_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFB2207326.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="JQH22_003080"
     CDS             887..2050
                     /locus_tag="JQH22_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFB2207327.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="JQH22_003081"
     CDS             2134..3759
                     /locus_tag="JQH22_003081"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFB2207328.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="JQH22_003082"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="JQH22_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HFB2207329.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="JQH22_003083"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="JQH22_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFB2207330.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="JQH22_003084"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="JQH22_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFB2207331.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="JQH22_003085"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="JQH22_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFB2207332.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="JQH22_003086"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="JQH22_003086"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFB2207333.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="JQH22_003087"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="JQH22_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFB2207334.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="JQH22_003088"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="JQH22_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFB2207335.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="JQH22_003089"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="JQH22_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFB2207336.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="JQH22_003090"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="JQH22_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFB2207337.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="JQH22_003091"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="JQH22_003091"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFB2207338.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="JQH22_003092"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="JQH22_003092"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFB2207339.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="JQH22_003093"
     CDS             complement(12356..12631)
                     /locus_tag="JQH22_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFB2207340.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="JQH22_003094"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="JQH22_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFB2207341.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="JQH22_003095"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="JQH22_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFB2207342.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="JQH22_003096"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="JQH22_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFB2207343.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="JQH22_003097"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="JQH22_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFB2207344.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15030)
                     /locus_tag="JQH22_003098"
     CDS             complement(14468..>15030)
                     /locus_tag="JQH22_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFB2207345.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3666 c         4050 g         3802 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg cagaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgtta
//
LOCUS       DADLDT020000102        14854 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000102 DADLDT020000000
VERSION     DADLDT020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.102"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQH22_003099"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQH22_003099"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFB2207346.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQH22_003100"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQH22_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFB2207347.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="JQH22_003101"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="JQH22_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFB2207348.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="JQH22_003102"
     CDS             2977..3060
                     /locus_tag="JQH22_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HFB2207349.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="JQH22_003103"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="JQH22_003103"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFB2207350.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="JQH22_003104"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="JQH22_003104"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFB2207351.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="JQH22_003105"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="JQH22_003105"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFB2207352.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="JQH22_003106"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="JQH22_003106"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFB2207353.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="JQH22_003107"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="JQH22_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="JQH22_003108"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="JQH22_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFB2207354.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="JQH22_003109"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="JQH22_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFB2207355.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="JQH22_003110"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="JQH22_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFB2207356.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="JQH22_003111"
     CDS             13186..14385
                     /locus_tag="JQH22_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFB2207357.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3699 a         3781 c         3911 g         3463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag gcgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catt
//
LOCUS       DADLDT020000103        14565 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000103 DADLDT020000000
VERSION     DADLDT020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.103"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..581
                     /locus_tag="JQH22_003112"
     CDS             <1..581
                     /locus_tag="JQH22_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFB2207358.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="JQH22_003113"
     CDS             complement(635..1357)
                     /locus_tag="JQH22_003113"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFB2207359.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELDGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="JQH22_003114"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="JQH22_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="JQH22_003115"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="JQH22_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFB2207360.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="JQH22_003116"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="JQH22_003116"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFB2207361.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="JQH22_003117"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="JQH22_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFB2207362.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="JQH22_003118"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="JQH22_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFB2207363.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="JQH22_003119"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="JQH22_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFB2207364.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="JQH22_003120"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="JQH22_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFB2207365.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="JQH22_003121"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="JQH22_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFB2207366.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="JQH22_003122"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="JQH22_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFB2207367.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="JQH22_003123"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="JQH22_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFB2207368.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14565)
                     /locus_tag="JQH22_003124"
     CDS             complement(13620..>14565)
                     /locus_tag="JQH22_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2207369.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3717 c         3316 g         3775 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgtccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DADLDT020000104        14479 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000104 DADLDT020000000
VERSION     DADLDT020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.104"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(113..379)
                     /locus_tag="JQH22_003125"
     CDS             complement(113..379)
                     /locus_tag="JQH22_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFB2207370.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="JQH22_003126"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="JQH22_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFB2207371.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="JQH22_003127"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="JQH22_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="JQH22_003128"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="JQH22_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFB2207372.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="JQH22_003129"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="JQH22_003129"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFB2207373.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JQH22_003130"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JQH22_003130"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFB2207374.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JQH22_003131"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JQH22_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFB2207375.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="JQH22_003132"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="JQH22_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JQH22_003133"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JQH22_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFB2207376.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="JQH22_003134"
     CDS             complement(12641..14095)
                     /locus_tag="JQH22_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFB2207377.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="JQH22_003135"
     CDS             complement(14092..>14479)
                     /locus_tag="JQH22_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2207378.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3652 c         3420 g         3546 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DADLDT020000105        14475 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000105 DADLDT020000000
VERSION     DADLDT020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.105"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(337..582)
                     /locus_tag="JQH22_003136"
     CDS             complement(337..582)
                     /locus_tag="JQH22_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207379.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JQH22_003137"
     tRNA            complement(1227..1302)
                     /locus_tag="JQH22_003137"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JQH22_003138"
     CDS             complement(1408..2115)
                     /locus_tag="JQH22_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFB2207380.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="JQH22_003139"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="JQH22_003139"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFB2207381.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="JQH22_003140"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="JQH22_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFB2207382.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JQH22_003141"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JQH22_003141"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFB2207383.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JQH22_003142"
     CDS             complement(7299..7574)
                     /locus_tag="JQH22_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFB2207384.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JQH22_003143"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JQH22_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFB2207385.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="JQH22_003144"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="JQH22_003144"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFB2207386.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="JQH22_003145"
     CDS             9534..9860
                     /locus_tag="JQH22_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFB2207387.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="JQH22_003146"
     CDS             10044..10763
                     /locus_tag="JQH22_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFB2207388.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="JQH22_003147"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="JQH22_003147"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFB2207389.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="JQH22_003148"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="JQH22_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..>14475)
                     /locus_tag="JQH22_003149"
     CDS             complement(13164..>14475)
                     /locus_tag="JQH22_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFB2207390.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3795 a         3472 c         3721 g         3487 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    13621 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    13681 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    13741 gtaaaaatgc cgcccagaat gccgcccaga atgccgccca tcacaatgaa caccagaagc
    13801 aaacttggta aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa
    13861 agcgggtagc cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt
    13921 aagatccctg gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc
    13981 agggaaaaca caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag
    14041 acgtttacag cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt
    14101 gtgccaccaa cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata
    14161 ttcgccagga cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg
    14221 aggttaatca aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa
    14281 aaggggattg ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg
    14341 attgccgcga tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt
    14401 gagaacgaaa tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc
    14461 gcgatataac tatcc
//
LOCUS       DADLDT020000106        13963 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000106 DADLDT020000000
VERSION     DADLDT020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.106"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JQH22_003150"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JQH22_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFB2207391.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JQH22_003151"
     CDS             2219..2767
                     /locus_tag="JQH22_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFB2207392.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JQH22_003152"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JQH22_003152"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFB2207393.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="JQH22_003153"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="JQH22_003153"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFB2207394.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQH22_003154"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQH22_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFB2207395.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH22_003155"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH22_003155"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFB2207396.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQH22_003156"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQH22_003156"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFB2207397.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="JQH22_003157"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="JQH22_003157"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFB2207398.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQH22_003158"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQH22_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFB2207399.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="JQH22_003159"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="JQH22_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFB2207400.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DADLDT020000107        13956 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000107 DADLDT020000000
VERSION     DADLDT020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.107"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JQH22_003160"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JQH22_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFB2207401.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JQH22_003161"
     CDS             complement(1604..1777)
                     /locus_tag="JQH22_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFB2207402.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JQH22_003162"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JQH22_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFB2207403.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JQH22_003163"
     CDS             3415..3744
                     /locus_tag="JQH22_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HFB2207404.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JQH22_003164"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JQH22_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HFB2207405.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JQH22_003165"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JQH22_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HFB2207406.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JQH22_003166"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JQH22_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFB2207407.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JQH22_003167"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JQH22_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFB2207408.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JQH22_003168"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JQH22_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFB2207409.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JQH22_003169"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JQH22_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFB2207410.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JQH22_003170"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JQH22_003170"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFB2207411.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3244 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADLDT020000108        13797 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000108 DADLDT020000000
VERSION     DADLDT020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.108"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JQH22_003171"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JQH22_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFB2207412.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JQH22_003172"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JQH22_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFB2207413.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JQH22_003173"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JQH22_003173"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFB2207414.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JQH22_003174"
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JQH22_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFB2207415.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JQH22_003175"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JQH22_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFB2207416.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JQH22_003176"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JQH22_003176"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFB2207417.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="JQH22_003177"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="JQH22_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFB2207418.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="JQH22_003178"
     CDS             7489..7638
                     /locus_tag="JQH22_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207419.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="JQH22_003179"
     CDS             7839..9422
                     /locus_tag="JQH22_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFB2207420.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JQH22_003180"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JQH22_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFB2207421.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRRNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JQH22_003181"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JQH22_003181"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFB2207422.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JQH22_003182"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JQH22_003182"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFB2207423.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..>13797
                     /locus_tag="JQH22_003183"
     CDS             13381..>13797
                     /locus_tag="JQH22_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFB2207424.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3192 a         3641 c         3494 g         3470 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagtatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctggcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgagtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg cggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    13681 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
    13741 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttgggg
//
LOCUS       DADLDT020000109        13592 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000109 DADLDT020000000
VERSION     DADLDT020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.109"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="JQH22_003184"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="JQH22_003184"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFB2207425.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="JQH22_003185"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="JQH22_003185"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFB2207426.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="JQH22_003186"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="JQH22_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFB2207427.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="JQH22_003187"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="JQH22_003187"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFB2207428.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="JQH22_003188"
     CDS             complement(5465..5662)
                     /locus_tag="JQH22_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFB2207429.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="JQH22_003189"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="JQH22_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFB2207430.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="JQH22_003190"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="JQH22_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFB2207431.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="JQH22_003191"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="JQH22_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFB2207432.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="JQH22_003192"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="JQH22_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFB2207433.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="JQH22_003193"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="JQH22_003193"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFB2207434.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="JQH22_003194"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="JQH22_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFB2207435.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..>13592)
                     /gene="amiB"
                     /locus_tag="JQH22_003195"
     CDS             complement(12646..>13592)
                     /gene="amiB"
                     /locus_tag="JQH22_003195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFB2207436.1"
                     /translation="PPPPPPVVAKRVETPAVVAPRVSEPARNPFKTESNRTTGVISSN
                     TVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDD
                     PMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNRSATGASVWVLSNRRA
                     NSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMI
                     SQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEA
                     IYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
BASE COUNT         3027 a         3897 c         3410 g         3258 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct ctttttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gataaccatc cctttgtaaa taagggcaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgta
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tg
//
LOCUS       DADLDT020000110        12908 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000110 DADLDT020000000
VERSION     DADLDT020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.110"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="JQH22_003196"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="JQH22_003196"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFB2207437.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="JQH22_003197"
     CDS             complement(1806..3224)
                     /locus_tag="JQH22_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFB2207438.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="JQH22_003198"
     CDS             complement(3378..3722)
                     /locus_tag="JQH22_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFB2207439.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JQH22_003199"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JQH22_003199"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFB2207440.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="JQH22_003200"
     CDS             complement(4712..5647)
                     /locus_tag="JQH22_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFB2207441.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="JQH22_003201"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="JQH22_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFB2207442.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="JQH22_003202"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="JQH22_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFB2207443.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JQH22_003203"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JQH22_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFB2207444.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="JQH22_003204"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="JQH22_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFB2207445.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="JQH22_003205"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="JQH22_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFB2207446.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="JQH22_003206"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="JQH22_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFB2207447.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JQH22_003207"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JQH22_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFB2207448.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="JQH22_003208"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="JQH22_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFB2207449.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="JQH22_003209"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="JQH22_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFB2207450.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3296 c         2950 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 agtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DADLDT020000111        12867 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000111 DADLDT020000000
VERSION     DADLDT020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.111"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            13..921
                     /locus_tag="JQH22_003210"
     CDS             13..921
                     /locus_tag="JQH22_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207451.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            1160..1219
                     /locus_tag="JQH22_003211"
     CDS             1160..1219
                     /locus_tag="JQH22_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFB2207452.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1536..1595
                     /gene="ibsE"
                     /locus_tag="JQH22_003212"
     CDS             1536..1595
                     /gene="ibsE"
                     /locus_tag="JQH22_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFB2207453.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1715..3148)
                     /gene="hldE"
                     /locus_tag="JQH22_003213"
     CDS             complement(1715..3148)
                     /gene="hldE"
                     /locus_tag="JQH22_003213"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFB2207454.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVEPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3196..6036)
                     /gene="glnE"
                     /locus_tag="JQH22_003214"
     CDS             complement(3196..6036)
                     /gene="glnE"
                     /locus_tag="JQH22_003214"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFB2207455.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6059..7360)
                     /gene="ygiF"
                     /locus_tag="JQH22_003215"
     CDS             complement(6059..7360)
                     /gene="ygiF"
                     /locus_tag="JQH22_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFB2207456.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7602..8222
                     /locus_tag="JQH22_003216"
     CDS             7602..8222
                     /locus_tag="JQH22_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFB2207457.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8286..9524
                     /gene="cca"
                     /locus_tag="JQH22_003217"
     CDS             8286..9524
                     /gene="cca"
                     /locus_tag="JQH22_003217"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFB2207458.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9687..10508)
                     /gene="bacA"
                     /locus_tag="JQH22_003218"
     CDS             complement(9687..10508)
                     /gene="bacA"
                     /locus_tag="JQH22_003218"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFB2207459.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10599..10967)
                     /gene="folB"
                     /locus_tag="JQH22_003219"
     CDS             complement(10599..10967)
                     /gene="folB"
                     /locus_tag="JQH22_003219"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFB2207460.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11072..11689
                     /gene="plsY"
                     /locus_tag="JQH22_003220"
     CDS             11072..11689
                     /gene="plsY"
                     /locus_tag="JQH22_003220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFB2207461.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11702..12633)
                     /gene="ttdR"
                     /locus_tag="JQH22_003221"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11702..12633)
                     /gene="ttdR"
                     /locus_tag="JQH22_003221"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         3098 a         3623 c         3209 g         2937 t
ORIGIN      
        1 caagaagcgt ttatgacgct tgagcaggag cagcaggtta aaacccgtac tgccgaacag
       61 aatgcacgta ttgcggcttt tgaagctgaa cgtcgtcgtg aagcagagca gacgcgaatt
      121 ctggctgaac gacaaattca ggaaacggag atcgagcgcg agcaggccgt ccgctcaaga
      181 aaggttgagg ctgaacgtga agttcgtatt aaagagatcg aacagcagca ggtcaccgaa
      241 atcgccaacc agacgaaatc gatcgctatt gccgccaaat cggaacaaca gtcccaggca
      301 gaagcgcgtg ctaaccttgc actcgcagaa gcagtaagtg cgcaacaaaa cgtagaaacc
      361 actcgccaga ccgcagaagc cgatcgtgct aaacaagttg ccctaatcgc tgccgcgcag
      421 gatgcagaaa ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa agaagccgca
      481 gagatgcagg cggcggctat cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg
      541 gaagcagaag cacaacgtgc gctgaacgat gctatcaacg tactttctga tgaacaaacc
      601 agccttaaat tcaaactggc cttgttgcag gcgctacctg cggtaataga aaaatccgtt
      661 gagccgatga aatctattga cggtatcaag attattcagg tcgatggtct gaatagtggc
      721 agcgctgcgg gtgatgcaaa cacgggtaat gtggagggcg gaaacctagc ggaacaagca
      781 ttatcagccg ctctctctta ccgcacacag gctccgctga ttgactcctt gctcaatgaa
      841 attggcgttt caggcggttc actggcggca ttgacttcac ccttaatctc aacaactccc
      901 gtcgccgaaa acgtaaaata aattttcagc tatgaacgtc cctggcacaa aaagccgggg
      961 attttttata tctgcgttcc gctaaaaact gcaaatgatc aggtcgttgc aacgttttgc
     1021 gtgaccgctc ggggaaggca aaactgcctc tgggaaagcg ttgcgcgagg tccggcgctc
     1081 atcaacaatc ggggagtcag caaagggctg aaacgggaaa gcccctcccg aggaaggggc
     1141 cttacagaag gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt
     1201 aagtttcgca gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt
     1261 cctttacttt aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca catatccctg
     1321 gcccaaaaag ccggggattt tttatatctg cgttccgcta aaaggtgcaa atgctcagga
     1381 cgttgcagcg ttttgcgtga ccgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc
     1441 gcggggtccg gcgctcatca acaatcgggg agtcagcaag gggctgaaac gggaaagccc
     1501 ctcccgagga aggggcctta tagaaggaaa gggttatgat gaagttcgtc atcatactgg
     1561 ttgtgttgtt actgttaagt ttcccgactt actaacaact gatcagaggg gggagaaatc
     1621 ctcccttacc cttgttcctt tactttaggt tgaaaaaaca acagcgtcaa taggcctgcc
     1681 tgctacgaag cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct
     1741 tcttgatgat gttagtggtc gagcaaccgt cttcgaagtt gagcaccagc acttcgccgc
     1801 cgttggccca gacttcttta ctcccggcga tctcttctgg tttatagtcg ccgcccttca
     1861 ccagtaaatc tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca
     1921 ccacccagtc gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttaa
     1981 cggggcgaga atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca
     2041 agcggtcacc cagtttgcgg gcatttgcca gatagaagac gtgtccggcg tgcaggatgt
     2101 caaagacgcc attggtcatc accacttttt caccgcgttt acgcgctgcc gctacggcca
     2161 gcttcagttc ctcttcggtc atcacgccaa agccggtatc tgcgcgccca cgcactgcat
     2221 tttccagctc gatcggcgaa accgtggagg ttcccagttt gccgaccacc acgccagccg
     2281 ccgcattggc aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca
     2341 ggacgccaat caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgggttg
     2401 gcatatgcag cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca
     2461 ctaacagagc cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt
     2521 cttcggtctt acatttaccg acaacagctt caaattccga gagattcggt gtcagcagcg
     2581 tagcgccgcg gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacgcccg
     2641 ctttacgcgc cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag
     2701 aaagcaccag cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg
     2761 gctcaacacc ttcgaaacct tcttcaaaat ccagacggat cagctgttgg ttgcgggaaa
     2821 gtacccgtaa tttggtgatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt
     2881 tgacgtcggc cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga
     2941 ccaggcgtgc attagcaccg agagaagcga tattcatcgc cacgttagcc gcgccgcccg
     3001 gacgttcttc gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacgac
     3061 tggtggggcc gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac
     3121 gttcaaactc tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg
     3181 cgataatacc atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagatc
     3241 acgctctgcg gtgaagcaat cctccgacac atggcccggc aattcctgta atgccagatg
     3301 atgaagttca tcgcgcagcg tcgtgtaagc acgggtcagc gccatcgctt cctgctcttc
     3361 cataatgtcg ttttgcgcca gcagctccag aatacgcacg ttgtctgacc agcgcgttaa
     3421 cttcggtttt tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt
     3481 aattccgcct tcatcggctt tgatatcaaa gcgattacga tgtttattgc cgagatgagc
     3541 gcgcattttc tcgcgcattt cccgcacttc cgtttgcaga gttttacctt ctcgcggcag
     3601 tgtcataatc tcacgacgca ccgcgtcaaa gtgcgcagtg agctgcggat cgccgtacac
     3661 tacacgcgca cgcaccagcg cctgatgttc ccacgtccag gcctcgtttt tctgataatc
     3721 ggcaaatgct tctgcggatg tcaccagcat tcccgccgcc ccggacggac gcagtcgagc
     3781 atccacttca tacaaaatgc cggaagaggt acgcgtactg aacagatgca taatgcgttg
     3841 cgccagacgc agataaaact gccgcccgtc gatttcccgc tcgccgtcag tcatcgcatc
     3901 cattgggcaa tcatggagga agataaggtc aagatcggaa ctgtagccta actcccagcc
     3961 gcccagcttg ccgtagccga ccaccgcaaa accgcgccct tcacgttcgt tgaggtgatt
     4021 tggcttaccg tagcgggcca ccatttgacc ccacgcctgc tgaacgacgg catctatcat
     4081 agcttctgcc agccaggtta agtgatcgct cactttcatt accggtagcg taccggcgat
     4141 atccgctgcc gcaatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg
     4201 ttgttcttcg tcatcttccg gcacgcgcag caaatactgg cgcagttcat cacggtaggc
     4261 atcggtcgcc gtcggctgat aaagggtatt tggatcgagc aattcatcca gcaataatgg
     4321 ataacgcgct agctggctgg caatcatcgg cgatgcggca cacagagaaa tcaaatgttt
     4381 aagcgccgca gggaattcac tgagcaattc taaataggtt gtgcgggtaa caatccccac
     4441 cagcaaggcg gtaatgcgcg acagcgtgac agcagcgtct tcacgcgcac agacatcact
     4501 tagcagatgt ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tagtacgctt
     4561 atccagctct ttgcggaaat cagcaatcag cgttaggacc tgtttgcgat catcctcgct
     4621 aagatgcgcc agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg
     4681 ccactgttcc gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt
     4741 aaacacccgg cgcacattgg tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc
     4801 agcaaagtcc atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtttg
     4861 ggtttgttcg tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatatgc
     4921 cactcgcaat tgttcagcat cgttttcaga aagcagatgc agcgcggcaa tggcgctgag
     4981 cgttggcagt aaagagcgcg actgcagcaa cggttcgcgc ccaccgcgaa tgagctggaa
     5041 cacctgaacg ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt
     5101 caaaccacgt cgacgcactt cacgggcaat catccctttc atgttgcgca gcgactgaat
     5161 cacgctgaaa tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt
     5221 agcatagacg ccttcgctat cgcccataat ccgcgctttg accatcgcgt aacgctccca
     5281 gtcgcgtccc tgctcctggt aataatcctc caacgcggca aagctcagca ccagcgggcc
     5341 actttcgcca aacggacgca gtcgcatatc cacgcgatag acgaagccat cctgcgttgg
     5401 ttgatccagc actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag
     5461 ttcccggcgt ccgccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc
     5521 agaggagaaa ttcagttccc cgccgcccag cttacccatg cctaagatca gcagcggttg
     5581 cgcttcgccc tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catacagcca
     5641 gtcacgcgcc gcaacaatca gcgtctccgc cagataactg agctgctgca atatgctctc
     5701 ttcagtaacc agtgccagcg tttgcgccca tgcgatgcgc accataatgc gccgccggaa
     5761 tagccgcagc tcgcgcatta acccggcttc gtcgctcaca ttactgagcg cctcctgcaa
     5821 ccacacgctg taatgttgcc attcgtcagc ctgtggcggt tggctttcca gttccgtcag
     5881 ccactctgga tgcgcaatca cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt
     5941 cgcctgtgca ctaagtgatt cctcggctaa aagctctggc agccgctcaa caacggtctg
     6001 ccagtactgc tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt
     6061 aacgttttcc actgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt
     6121 caatttcaat gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag ttctccagcc
     6181 aggcttgcgc gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac
     6241 gcatcaggcg tggcaactgg tcacggtagc gatcgcctaa cggctggcag aaaacgcttt
     6301 tcagttcagc ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctaa
     6361 ttttgccctg cgctttggca tctaaaaacg gctgccatgc tttgcttacc agccattcgg
     6421 tcaacgccag cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg
     6481 cagaagcgat ggtcgcttcg cattgagtca gcaaatcacg taagtgagta ctcgctttac
     6541 gcggcacaat gccaccgaac agcatcagtg tatgacggac taggctaatg gctgccagca
     6601 cctgctcttt cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg
     6661 ctaacaccag ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat
     6721 gcaaaatggt ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac
     6781 gcgccgcttt gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca
     6841 gtttcagcac cgcgcgcgtg tcgccgctaa gcagctccag ttccagctcg cagataggct
     6901 cagcaaattc acccgctttc acttccccct ggtcgagagc gatttcaatt cggctgccat
     6961 tgatctctac tagccatttt tcgcgataaa aatcggtgct aaacagcgac tgcacgcggg
     7021 aagcgagatc ggcaggcaat tcgccgttcg gccagatttc cgtcggtaac tgcgccaggt
     7081 cgagcgtcgg ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca
     7141 ctcttcctgc aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc
     7201 ccatatcgtg cctacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca
     7261 cggggtcatg gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac
     7321 tgtgattaac aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat
     7381 gtcacataag tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat
     7441 tttgcgccaa attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc
     7501 aaggcctgac acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc
     7561 actactatcg ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga
     7621 tcggattaac tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct
     7681 atgtttctga cgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg
     7741 tgggcacggt taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt
     7801 atgcccaggt gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca
     7861 ctgagccaag cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg
     7921 ataaactcac caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag
     7981 tggcgcagag cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg
     8041 agctgattgt cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc
     8101 gcaccatcat catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc
     8161 tcggtctggt actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact
     8221 aaatcgcctt cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg
     8281 atggcgtgaa gatttatctg gtcggtggtg ctgttcggga tgcattgtta ggactatcgg
     8341 tcaaagacag agattgggtg gtggtcggca gtacgccaca ggagatgctc gacgcgggct
     8401 accagcaggt aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg
     8461 cgctggcacg taccgaacgg aaatccggtt ccggttacac cggttttact tgctatgccg
     8521 caccggatgt cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg
     8581 cccaggacga taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc
     8641 gtctgttgcg ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg
     8701 cgcgttttgc tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc
     8761 tgatgcgcga gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga
     8821 aagagacgga aagcgccctc accacccgca atccccaggt gttcttccag gtactgcgcg
     8881 attgcggcgc acttcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggcac
     8941 ctgccaggtg gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg
     9001 cggcgatgct gagtccgcag gttgatgtcc gtttcgcaac tttatgccac gatctcggta
     9061 aaggactgac gccgccagaa ctttggccgc gtcatcatgg gcatggcccg gcgggtgtta
     9121 agttagtgga acaactatgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca
     9181 gactggtggc tgagtttcac gatctcatcc gcaccttccc aatgctgaac ccaaaaacca
     9241 tcgtcaaatt atttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg
     9301 cgctgaccag cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc
     9361 aaggccgttg gttgcgcgaa ggctgggaag tggcgcagtc agtgccgaca aaagcggtcg
     9421 ttgaagcggg atttaaaggc gtggagattc gcgaggagct gacccgacgg cggattgcgg
     9481 cggtagccag ctggaaggaa caacgttgcc caaagcctga ctgagtataa aactaaacgt
     9541 tagcacgaac cgtcccctcg ccccttcggg gagagggtta gggtgagggg aacagaccag
     9601 caccggtgcg aacaatcacc cccaccccgg ccctctccct acagagcgag ggagaaaacc
     9661 cggcctgctc ccgaaccttt tctcaatcaa aagaacacga catacaccgc agccgccaca
     9721 ataaagcgat aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt
     9781 ttaatcgcta tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata
     9841 tcgccggttg tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc
     9901 atcggtaccg ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc
     9961 atcccacctg aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg
    10021 aaacagccaa tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc
    10081 tctttcggct tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagcgcatac
    10141 atcacattta tcgggttaaa caatgactta atcgtgtcgt ggaataatag ccccagtacc
    10201 accgccggaa tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttcacct
    10261 tcgtgctgca acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac
    10321 atcactacta ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg
    10381 tcgccctcaa accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc
    10441 agaaattctg tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata
    10501 tcgctcatca ataaacccct aaattattaa aatgtaccgc ttgtccgaac tactgcagta
    10561 tgaccaggtt ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta
    10621 ttgccacgct caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg
    10681 agtttgatac gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca
    10741 gccacgcgtt ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca
    10801 atgtcagcgt aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc
    10861 catgccattt cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca
    10921 ccaatagtgg tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg
    10981 ctttttggct aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg
    11041 ttgaacacga attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct
    11101 catcgcgtac ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct
    11161 gcccgatccg cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg
    11221 tggcaaggga gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcctgt
    11281 ctggggcgcg tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc
    11341 ctgtcttgga cacatctggc ccgttttctt cggttttaaa ggaggaaaag gcgttgctac
    11401 cgcttttgga gcaatcgcac caattagctg ggatctcacc ggagtaatgg cgggaacctg
    11461 gttactgacc gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat
    11521 tgctccgttt tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc
    11581 ttgcctgatc ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac
    11641 aaaaatctgg acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga
    11701 tctacatgac ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca
    11761 gaaattccgc gcagacacga atttcatgct gcgatagagc agctcccggt aaacagccca
    11821 gatattggcg ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa
    11881 cggcaacaca tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcaa
    11941 cactatctcg ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg
    12001 accgttcccc aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc
    12061 atgacgactt aattcctgta atgattgtgg ctgcggatat ttttgcagat actcaggtgc
    12121 tgcgcataat attcttttat ttttcgttaa cagatgcgca atataataat caggaatttc
    12181 gtcattaata cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa
    12241 caattcaaaa tgcacctgta actcaggata attgcgcata agttcggtaa tggctggcgc
    12301 aatatggctg cgcccaaaac caaaactgca accaatacga atcatcccct ccgggcgcgt
    12361 tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg
    12421 ttcgtaacaa cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag
    12481 cgttgttgcc agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtttg
    12541 cccaagcgtc gccgctgcag cactaaaact cccgctgtgg acgatttcta ccagtacctg
    12601 caagtctttg gctaaaggcc agctattcag catcgttaat tatccgcagt tgtgataagc
    12661 gcagtgtatt tcgcaaaaca ttgccattcc ctgctctggc gcaatattca ttcttatttt
    12721 cgcgttgatt cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat
    12781 ttttagtgtt agtttgtggc ggtgaatttt tccagacaaa tacaaaaact ggagttgcca
    12841 tgatgagcga aagtaataag caacagg
//
LOCUS       DADLDT020000112        12695 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000112 DADLDT020000000
VERSION     DADLDT020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.112"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQH22_003222"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQH22_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFB2207462.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JQH22_003223"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JQH22_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFB2207463.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JQH22_003224"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JQH22_003224"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFB2207464.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JQH22_003225"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JQH22_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HFB2207465.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JQH22_003226"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JQH22_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFB2207466.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JQH22_003227"
     CDS             4025..4615
                     /locus_tag="JQH22_003227"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFB2207467.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JQH22_003228"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JQH22_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HFB2207468.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JQH22_003229"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JQH22_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFB2207469.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JQH22_003230"
     CDS             complement(6551..6658)
                     /locus_tag="JQH22_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207470.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="JQH22_003231"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="JQH22_003231"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFB2207471.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="JQH22_003232"
     CDS             7648..7875
                     /locus_tag="JQH22_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFB2207472.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQH22_003233"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQH22_003233"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFB2207473.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRVSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="JQH22_003234"
     CDS             complement(9447..10703)
                     /locus_tag="JQH22_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFB2207474.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSVQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="JQH22_003235"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="JQH22_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFB2207475.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="JQH22_003236"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="JQH22_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFB2207476.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQH22_003237"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQH22_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFB2207477.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3035 a         3245 c         3196 g         3219 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactgacgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt aacgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggacac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DADLDT020000113        12565 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000113 DADLDT020000000
VERSION     DADLDT020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.113"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(76..795)
                     /locus_tag="JQH22_003238"
                     /pseudo
     CDS             complement(76..795)
                     /locus_tag="JQH22_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(811..1143)
                     /gene="cusF"
                     /locus_tag="JQH22_003239"
     CDS             complement(811..1143)
                     /gene="cusF"
                     /locus_tag="JQH22_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFB2207478.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1167..2549)
                     /gene="cusC"
                     /locus_tag="JQH22_003240"
                     /pseudo
     CDS             complement(1167..2549)
                     /gene="cusC"
                     /locus_tag="JQH22_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            2706..3389
                     /gene="cusR"
                     /locus_tag="JQH22_003241"
     CDS             2706..3389
                     /gene="cusR"
                     /locus_tag="JQH22_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFB2207479.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3379..4821
                     /gene="cusS"
                     /locus_tag="JQH22_003242"
     CDS             3379..4821
                     /gene="cusS"
                     /locus_tag="JQH22_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFB2207480.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4971..7208
                     /gene="nrfB"
                     /locus_tag="JQH22_003243"
                     /pseudo
     CDS             4971..7208
                     /gene="nrfB"
                     /locus_tag="JQH22_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7195..10163
                     /gene="nfrA"
                     /locus_tag="JQH22_003244"
                     /pseudo
     CDS             7195..10163
                     /gene="nfrA"
                     /locus_tag="JQH22_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10164..11054
                     /locus_tag="JQH22_003245"
     CDS             10164..11054
                     /locus_tag="JQH22_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFB2207481.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11237..11998
                     /gene="envY"
                     /locus_tag="JQH22_003246"
     CDS             11237..11998
                     /gene="envY"
                     /locus_tag="JQH22_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFB2207482.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12044..12120)
                     /locus_tag="JQH22_003247"
     tRNA            complement(12044..12120)
                     /locus_tag="JQH22_003247"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12084..12086),aa:Arg,seq:tct)
     gene            12363..>12565
                     /locus_tag="JQH22_003248"
     CDS             12363..>12565
                     /locus_tag="JQH22_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFB2207483.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3057 a         3272 c         3199 g         3037 t
ORIGIN      
        1 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
       61 acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga tattcattcc
      121 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
      181 ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc ccgccagtcg
      241 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcaacagtaa
      301 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt
      361 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
      421 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
      481 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
      541 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
      601 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
      661 ccagaataag attttacgtt ctgcggtcgg cattttttct gtgtgcggtt ccgcctggtc
      721 aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc cgataataag
      781 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
      841 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
      901 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tgacggcagc
      961 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt ttaccacacc
     1021 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
     1081 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
     1141 catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa ggcggtatac
     1201 aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca
     1261 aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg ctgatataat
     1321 gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg ggcgctgatt
     1381 tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa ggcgttctgg
     1441 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
     1501 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
     1561 ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
     1621 aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt agccgccatt
     1681 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
     1741 gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg cggtagcttg
     1801 ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
     1861 ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
     1921 ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
     1981 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
     2041 gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt ggctaaataa
     2101 ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag atcaaagctg
     2161 gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
     2221 cagctgccgc tgccctagcc attgagctgt gggtagcggt cggcatcggt cagacgatat
     2281 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc
     2341 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccacgttc
     2401 tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
     2461 ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc aagggccaca
     2521 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgtcgc
     2581 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
     2641 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
     2701 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
     2761 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
     2821 tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca gacgtgaacg
     2881 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
     2941 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca gatgactacc
     3001 tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
     3061 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
     3121 gccgcaaagt caccctcagc ggcacgcgca tcactctgac cagtaaagaa tttactctgc
     3181 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
     3241 tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg aagcggctac
     3301 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
     3361 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
     3421 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
     3481 ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt aaaagagatt
     3541 agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
     3601 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
     3661 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
     3721 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
     3781 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgccg
     3841 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct gtcgatcgat
     3901 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
     3961 agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc
     4021 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
     4081 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
     4141 attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
     4201 acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
     4261 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
     4321 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
     4381 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gctagcggaa
     4441 gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg cgatccgttg
     4501 atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac gccaaccaga
     4561 gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc cgtcgaaaac
     4621 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt
     4681 gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat agtgaaatcg
     4741 attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt
     4801 gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc agtgtaaaaa
     4861 taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt cataaaaaac
     4921 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
     4981 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
     5041 tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
     5101 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
     5161 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     5221 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     5281 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     5341 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgcc
     5401 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     5461 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     5521 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa cgcgaatgga
     5581 cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc aaagatgttc
     5641 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     5701 gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac gtgcagagtc
     5761 ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg gaaatttttg
     5821 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     5881 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcgg
     5941 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccataaat
     6001 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     6061 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     6121 aaagtttgtg gcccgatgcc tagcatttcc tttctatttt tagcggcagc gcatggttaa
     6181 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgagtgattt
     6241 tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt cttttctggg
     6301 gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt cagcacggcg
     6361 atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc actggcgata
     6421 cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc actgaagaac
     6481 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     6541 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     6601 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccagcct
     6661 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     6721 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     6781 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc cactggtatg
     6841 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     6901 tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     6961 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     7021 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     7081 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     7141 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     7201 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     7261 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     7321 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     7381 gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga taatattccg
     7441 ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg ggcgcggctg
     7501 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     7561 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     7621 gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa taccctgcgg
     7681 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgccg
     7741 gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatgatcc
     7801 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     7861 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     7921 tcacagggga tcttcactga tcctcagtca tatattactt acgcgaccgc gctggcttat
     7981 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     8041 ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     8101 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     8161 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     8221 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     8281 gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct tccccgcctg
     8341 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     8401 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     8461 gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc gattttatcg
     8521 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     8581 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgctgccgcc
     8641 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     8701 cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc ctatcaggcg
     8761 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     8821 atgagtaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg aaatggcgcg
     8881 gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa tgctcaatac
     8941 tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact gaacgatctc
     9001 acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc gacaatttat
     9061 cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct ggaactggaa
     9121 ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
     9181 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
     9241 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
     9301 gcccggctcg tgattgatga cattgataat caggcataac cccactgacc ccagagcaaa
     9361 atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc tggacgttca
     9421 gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac aataatgtcg
     9481 gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc gagtaccgca
     9541 tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc gtctttgccg
     9601 ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc accggtctgc
     9661 gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag ttgccgctga
     9721 acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc tttaatggcg
     9781 gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa aacctgtacc
     9841 tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat tatcgcgtca
     9901 gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac gttcaggaca
     9961 acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg gtccgctgga
    10021 atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt ctcggcgtcg
    10081 agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac aacgcgtttc
    10141 tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg acactgcttt
    10201 tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa aaccgagata
    10261 gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt caaggcttcg
    10321 ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca gaacagcgca
    10381 cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg attgtcgggc
    10441 tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg ctggaaagct
    10501 atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc gccgcgcctg
    10561 gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac tggcgcagcg
    10621 aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg attagcgatg
    10681 tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg cccgatggct
    10741 atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta caggatggca
    10801 gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc agcgccgatt
    10861 gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc ggcaaaggca
    10921 aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta gcgaaacgtt
    10981 cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc gcgcacggca
    11041 ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac ctctttcgct
    11101 ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag aagatcgtta
    11161 tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg agtttctgat
    11221 atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga tcctgaccga
    11281 aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga aaaactacct
    11341 ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc acgtattaat
    11401 cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata agaaattaac
    11461 ctgtgtaaag ccctggtcgc ggctggcaac cccggttatc gcttgtcata gccgtacacc
    11521 ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac cctgcgaagc
    11581 ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc aatcgcggtt
    11641 tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct gccgcattat
    11701 tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc tatgtttaag
    11761 ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc agattgtcac
    11821 agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa atatcactca
    11881 ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt ttaaggcgtt
    11941 ttacagcctg acaccgttga attatctcgc caaacagcga gaaaaagtga tgtggtgaag
    12001 ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg ccctgcagga
    12061 ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag ctaagggcgc
    12121 gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg ctgagtcaat
    12181 ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg atttatttcg
    12241 ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc cgtattttca
    12301 tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg agactggtaa
    12361 ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga atgtctattg
    12421 aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat gattaccgca
    12481 taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat atagacttgc
    12541 ccggaacaga cggttttacc ttcct
//
LOCUS       DADLDT020000114        12413 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000114 DADLDT020000000
VERSION     DADLDT020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.114"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1198
                     /gene="edd"
                     /locus_tag="JQH22_003249"
     CDS             <1..1198
                     /gene="edd"
                     /locus_tag="JQH22_003249"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFB2207484.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1235..1876
                     /gene="kdgA"
                     /locus_tag="JQH22_003250"
                     /gene_synonym="eda"
     CDS             1235..1876
                     /gene="kdgA"
                     /locus_tag="JQH22_003250"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFB2207485.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1932..3110)
                     /gene="purT"
                     /locus_tag="JQH22_003251"
     CDS             complement(1932..3110)
                     /gene="purT"
                     /locus_tag="JQH22_003251"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFB2207486.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3244..3534
                     /gene="yebG"
                     /locus_tag="JQH22_003252"
                     /pseudo
     CDS             3244..3534
                     /gene="yebG"
                     /locus_tag="JQH22_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3601..3957
                     /locus_tag="JQH22_003253"
     CDS             3601..3957
                     /locus_tag="JQH22_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFB2207487.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4284..4943
                     /locus_tag="JQH22_003254"
     CDS             4284..4943
                     /locus_tag="JQH22_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFB2207488.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5153..7213
                     /gene="ptrB"
                     /locus_tag="JQH22_003255"
                     /pseudo
     CDS             5153..7213
                     /gene="ptrB"
                     /locus_tag="JQH22_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="JQH22_003256"
     CDS             complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="JQH22_003256"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFB2207489.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7896..8552)
                     /locus_tag="JQH22_003257"
                     /pseudo
     CDS             complement(7896..8552)
                     /locus_tag="JQH22_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8654..8884)
                     /gene="holE"
                     /locus_tag="JQH22_003258"
     CDS             complement(8654..8884)
                     /gene="holE"
                     /locus_tag="JQH22_003258"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFB2207490.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9023..9397
                     /gene="yobA"
                     /locus_tag="JQH22_003259"
     CDS             9023..9397
                     /gene="yobA"
                     /locus_tag="JQH22_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFB2207491.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9401..10273
                     /gene="copD"
                     /locus_tag="JQH22_003260"
                     /pseudo
     CDS             9401..10273
                     /gene="copD"
                     /locus_tag="JQH22_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10286..10627
                     /locus_tag="JQH22_003261"
     CDS             10286..10627
                     /locus_tag="JQH22_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFB2207492.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11020..12096)
                     /locus_tag="JQH22_003262"
                     /pseudo
     CDS             complement(11020..12096)
                     /locus_tag="JQH22_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12062..12343)
                     /locus_tag="JQH22_003263"
     CDS             complement(12062..12343)
                     /locus_tag="JQH22_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFB2207493.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3079 a         3032 c         3137 g         3165 t
ORIGIN      
        1 ggccttactg gagtcagaag ccgcgtctta ccatgcgccg ggaacatgta ctttctacgg
       61 tactgccaac accaaccaga tggtggtgga atttatgggg atgcagttgc caggctcttc
      121 ttttgttcat ccggattctc cgctgcgcga tgctttgacc gctgccgctg cgcgtcaggt
      181 tacacgcatg accggtaatg gtaatgaatg gatgccgatc ggtaagatga tcgatgagaa
      241 agtggtggtg aacggtatcg ttgcactgct ggcgaccggt ggttccacta accacaccat
      301 gcacctggtg gcgatggcgc gcgcggccgg tattcagatt aactgggatg acttctctga
      361 cctttctgat gttgtaccgc tgatggcacg tctctacccg aacggtccgg ccgatattaa
      421 ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt gaactgctca aagcaggcct
      481 gctgcatgaa gatgtcaata cggtggcagg ctttggtctg tctcgttata cccttgaacc
      541 atggctgaat aatggtgaac tggactggcg ggaaggggcg gaaaaatcac tcgacagcaa
      601 tgtgatcgct tccttcgaac aacctttctc tcatcatggt gggacaaaag tgttaagcgg
      661 taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg gttgagaacc aggtgattga
      721 agcgccagcg gttgtttttg aaagccagca tgacgttatg ccggcctttg aagcgggttt
      781 gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg ccaaaagcga acggaatgcc
      841 agaattacat aaactcatgc cgccacttgg tgtattattg gaccggtgtt tcaaaattgc
      901 gttagttacc gatggacgac tctccggcgc ttcaggtaaa gtgccgtcag ctatccacgt
      961 aacaccagaa gcctacgatg gcgggctgct ggcaaaagtg cgcgacgggg acatcattcg
     1021 tgtgaatgga cagacaggcg aactgacact gctggtagac gaagcggaac tggctgctcg
     1081 cgaaccgcac attcctgacc tgagcgcgtc acgcgtggga acaggacgtg aattattcag
     1141 cgccttgcgt gaaaaactgt ccggtgccga acagggcgca acctgtatca ctttttaaga
     1201 cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa aactggaaaa caagtgcaga
     1261 atcaatcctg accaccggcc cggttgtacc ggttatcgtg gtaaaaaaac tggaacacgc
     1321 ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc gttctggaag tgactctgcg
     1381 taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa gaagtgcctg aagcgattgt
     1441 gggtgccggt acggtgctga atccacagca gctggcagaa gtcactgaag cgggtgcaca
     1501 gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa gctgctaccg aagggactat
     1561 tcctctgatt ccggggatca gcactgtttc cgaactgatg ctgggtatgg actacggttt
     1621 gaaagagttc aaattcttcc cggctgaagc taacggcggc gtgaaagccc tgcaggcgat
     1681 cgcgggtccg ttctcccagg tccgtttctg cccgacgggt ggtatttctc cggctaacta
     1741 ccgtgactac ctggcactga aaagcgtgct gtgcatcggt ggttcctggc tggttccggc
     1801 agatgcgctg gaagcgggcg attacgaccg cattactaag ctggcgcgtg aagctgtaga
     1861 aggcgctaag ctgtaaaagt caaaatgccc gatctaggat cgggcatttt tgtagcgttt
     1921 ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc gcgttcaatg
     1981 gcgtcaacaa cactctctga agtagccagt accaccccca gacgacggct gccatcaatt
     2041 tccggcttac caaataaacg aatctgcaaa tctgcgccta cggcattctg cacattatca
     2101 aacgtgacat tctgactggt cagttgtggc agaataacgg cagaagctgc aggaccatac
     2161 tgacggatcc cgccaacagg aagtccgagg aaggcacgta catgcagggc aaactctgag
     2221 agatcttgag aaattaacgt caccatcccg gtatcatgtg gacgagggga gacctcactg
     2281 aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagaccata accgcccagt
     2341 gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg gctcatttgc
     2401 tgtggttgcc aggattcacg gtagtcgcca tcttcctggc gatgacctac tggtgcacag
     2461 aaatggacgc catccaccgc gctgacggtt agcagggtaa tttcgaagtc aaacttaacg
     2521 acgccttcaa caattacgcg gcccgctccg gcgcgaccgc cttgctgagc gtattcccat
     2581 gcctgagcaa gttgctctgc agagcgaata aacgtctgcc ccttgccgga agagctcatc
     2641 accggtttta caatgcaggg atagccaatg gcggcaaccg cctcgcggaa aaggcttttg
     2701 ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc caggcgacgg
     2761 ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc aggggacaac attcagtcct
     2821 tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg cacgatataa
     2881 tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat attaatgaca
     2941 tgggagcgat gcgcgacatg catggctggt gcgtcggcat agcgatcgac ggcaatcacc
     3001 tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc ggagcctaat
     3061 aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat aacgaatatc
     3121 ccctgattaa ttccgctcgc agtataaacg aaaacgtttg cgtgtgtctt tattgctgat
     3181 gttgatttct caaccgaaaa gaaatatact gtataaaatc tcagttatta tgagaggctg
     3241 caaatggcgg ttgaagttta atacgtagtc attcgtgagg gcgaagagaa aatgtcgttt
     3301 accagcaaaa aggaagccga tgcttatgac aaaatgcttg atacggcgga tctgcttgac
     3361 acctagctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc cctttcgcta
     3421 tggctggcag aacaaaaaga ggtgctgagc accattctga aaaccggcaa attaccgtcc
     3481 ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc ctgaagcaac
     3541 ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat ggagaaaaac
     3601 atgaaaaaaa gaggggcgtt tttagggcgg ttgttggttt ctgcctgcgc atcagttttc
     3661 gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg tctggatgct
     3721 gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa atcgcgtggc gcgctgggca
     3781 gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct gggtcagttt gcaggacatt
     3841 cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag tgccgatatt
     3901 cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg gcgttaatgg
     3961 cttctgataa ttcgattgac cgttggcgga tgcggcgcga gtgccttgtc cgtcccagaa
     4021 cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg catcgggcaa tcagcatcaa
     4081 acaatgccac tcaaaacgac aaactacttt cacctaaaat acccttatca cttgatatac
     4141 agtaaatacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc cgcttatttg
     4201 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
     4261 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
     4321 ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg attgggcaaa ctgttagtgt
     4381 caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cgtaaacttc
     4441 ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtagcg ggtacggtgc
     4501 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
     4561 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
     4621 ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc aatcgaccag caattgcgtg
     4681 aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggcaatcgaa caaccgctgg
     4741 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
     4801 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
     4861 gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat tgaacgcgat ctcgaacagc
     4921 aaaaacgcac tttggcggaa taattccgct ctctggatag cctgaagttg actatttcgg
     4981 gctatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc ataacattcg
     5041 atatccgcgc cattacctgt catcatctaa gcaatgacga ccctgtttcg cttgcatccc
     5101 cggtgagttt tgccaccctt ataagatgtt tccaaccaga aagaacaata acatgctacc
     5161 aaaagccgcc cgcattcccc acgccatgac gcttcatggc gatacgcgca tcgataatta
     5221 ctactggctg cgggacgata cgcgttctca gccaaaagtc ctggactacc tgcaacaaga
     5281 aaatagttac ggtcatcggg tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa
     5341 ggaaatcatc gaccgcattc cgcaaagaga agtttctgcg ccctacataa aaaatggcta
     5401 ccgctatcgg catatttatg aaccaggctg tgaatatgct atctaccagc gtcaatcggc
     5461 attcagcgaa gagtgggatg agtgggaaac attgctcgat gccaacaagc gcgcagctca
     5521 tagtgagttt tattcgatgg gcggaatggc gattacgccc gataacacca ttatggcgct
     5581 ggcagaagat tttctttccc gacgccagta cggcattcgt tttcgtaatc tggaaacagg
     5641 taactggtac ccggaactgc tggataacgt tgaacccagc tttgtctggg caaatgactc
     5701 ctagactttc tactatgttc gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg
     5761 tcacgccatc ggtacgccag catcgcaaga tacactgatc tacgaagaaa aagacgatac
     5821 ctattatgtc agcctgcata agacgacgtc gaagcactat gtcgtcattc atctggccag
     5881 cgccaccacc agtgaagttc gcctgctgga cgcggaaatg gccgatgccg agccgtttgt
     5941 ttttctgccg cgccgcaaag atcacgaata cagccttgat cactaccagc atcgttttta
     6001 tctgcgttcc aatcgccacg gcaaaaactt tggcttatac cgtacccgta tgcgtgatga
     6061 gcaacagtgg gaagagttaa ttccgccacg cgaaaacatc atgctggaag ggtttacgct
     6121 gtttaccgac tggctggtgg ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat
     6181 taaccgcaag acccgggaag tcattggcat tgcctttgat gatccggcct atgtgacctg
     6241 gattgcctac aatccagaac ctgaaaccgc gcgattgcgt tatggttatt cttccatgac
     6301 cacaccagac actttgtttg aactggatat ggataccggt gagcgtcgtg tattaaaaca
     6361 aacagaagtg cctggttttg atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc
     6421 ccgtgatggc gtcgaagttc cggtttcgct ggtctatcat cgcaaacatt ttcgcaaagg
     6481 acacaacccg ttgctggtgt atggctatgg ttcttacggc gcaagtattg atgccgattt
     6541 cagttttagc cgcttgagtt tgttagatcg tggctttgtc tacgccattg tccatgttcg
     6601 cggcggtggt gagctggggc aacaatggta cgaagacggt aaatttctga agaagaaaaa
     6661 tacgtttaat gattatcttg atgcctgcga tgcattgtta aaactgggct atggctctcc
     6721 ttcgctctgt tatgcgatgg gcggaagtgc ggggggcatg ttgatgggcg ttgcaattaa
     6781 tcaacgcccg gaattattcc acggcgttat cgcccaggta ccgtttgttg atgttgtaac
     6841 aacgatgctt gatgaatcaa ttcctcttac cactggtgag tttgaagagt ggggaaatcc
     6901 gcaggatccg caatattacg agtacatgaa aagctacagc ccgtatgaca acgtcaccgc
     6961 acaggcttat ccgcatttac tggtaacgac cggtttacac gattctcagg tgcaatattg
     7021 ggaaccggca aaatgggtcg ctaaattgcg cgagctgaaa accgatgacc atcttttatt
     7081 gctctgtacc gacatggact caggccatgg cggtaaatct ggccgcttta aatcgtacga
     7141 aggcgtagcg atggaatatg cttttctggt cgcgctggcg cagggaacat tacccgctac
     7201 gcctgcggat taagtatttt ccagataatg tttcagtgtt aaacgcagct ccgggctcat
     7261 gctgttcagg ttattaaata accagcgcag atagcccgga tagcgttcgg caacgtcgga
     7321 aaccgctttg ccacggtatt tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt
     7381 gatatcggcc atctgttctg ccgtccagcc ggaggtgttc ataatatcga taagcaacgc
     7441 ggcggtgata taacagtcat acaacgcgcg gtgatgatgc aggcccggcg gtgtctgtac
     7501 attgagcttg cgtgttttat ataacgccat attgctgtac ttgagccctg gccacaaacg
     7561 acgggccagt ttcatagtgc aaatccactc accgggcatc tcaggcagta cgcggcggtc
     7621 aaagctggcg ttgtgcgcga cataccattc actaccgtaa tagtgtggga tcacatcttc
     7681 aatccacggt ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg
     7741 actaatagga cgatcggggc gcaccaggag gctcatgggg ttgacgattt ttccgtcaat
     7801 gacatcaaca gaggcaatct caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat
     7861 aatgcgcaac atggcatgct ccaggccgaa aaagcctagc gtaatggaat gatatcgcct
     7921 tgccaaccct gtgatgaacg ctgaccgact aacaataatg aaccacgatc ggcgcgaacg
     7981 atgagacgac catattcatc ccatagtgca ctgttaccac gggcatttgc catcagcacg
     8041 gcaatcgagt actgatgtga aaaaaattgt aagcgacggg ccgacgccag caggtcgggt
     8101 tctcccagac attgccctgt cgtaaacagt gaacaggtcg ggtccatatc cataccttgc
     8161 ggttgttcat cgaccaccgt gatggttctg gaacgacggc ccagacatgc accatgactc
     8221 tgatggtagt tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc acgaataaag
     8281 cgatcgttat attcaacggg aaggccggca atgatggtca ttcgctaggt cgttgcggca
     8341 taacaaagcg ggtctaacag tgaaagatcg ggtggggcag gaagggcacg tcgcgaataa
     8401 tcacacccca gtaaagaaag cgatggaaaa actaacagct gacattgttg cctggcggcg
     8461 gccctcacaa actccagatg atgagctacc tgttcggtga gtgaggtttt acgaggctca
     8521 tattgcgctg ctgcaacttt ccagaacgac ataatgactt ccttttcaaa atgactgcaa
     8581 attatggggg cggtttagaa aatactcata atttagcagg cgttatgtaa gaaagtgtaa
     8641 ctctgtataa gttttattta agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag
     8701 ccaaacggtg ggcaataagc cgctcgcgaa accagctgcg caaatgttct ggctgttcac
     8761 gttcaaccgc ttcagcgatc accggcatat tgtagcgttc tttaaaagcc accccggccg
     8821 ccgccaaatc gacattcact ttatccattt ctgtttgatc cagtttagcc agattcttca
     8881 gcattcttaa tctcctcatc attcgcgggc aaaagttact aagagtgagc ctggatggca
     8941 agccggaatt gatgatcctc gactcagaca ttagacaagg ttatgctctg taaaaaagtt
     9001 attacaaaaa ggaatagata atatggcttc aactgcacgc tcccttcgct acgcgctggc
     9061 aatcctgacc acttcgttgg tgacaccttc cgtctgggca catgcgcatt taacgcatca
     9121 gtatcctgcg gcaaacgcgc aagtgacagc tgccccgcag gcaatcactt taaacttctc
     9181 ggaaggcgtt gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat
     9241 taaaacatta ccagcgaagc ggaatgagca ggaccaaaag caactgattg ttccactggc
     9301 cgattcgctg aaacccggaa cctacaccgt tgactggcat gttgtttcgg tggacgggca
     9361 taaaacgaaa ggacactaca cctttagcgt gaaataaatc atgctggcgt ttacctggat
     9421 cgcgctgcga tttatccatt tcacctcgtt gatgctggtc tttggctttg caatgtatgg
     9481 tgcctggctg gcccccttaa tgattcgtcg cttgctgacg aagcgttctc tgcgtttaca
     9541 acagcacgct gccgtctgga gtttgatcag tgccactgca atgctcgccg ttcagggcgg
     9601 gttgatggga acgggatgga cagatgtatt ttcgccaaac atttggcagg ctgttttgca
     9661 aacgcagttt ggtggcgtct ggttatggca aattgttctc gccctcgtaa cgttgatcgt
     9721 cgcccttatg caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt
     9781 tattctgctg gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacag cgaaaatcca
     9841 tcagactaat cacgcgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc
     9901 ggtgctctgg tgtatgcaac tcatcaaagg ccgttggcga cactaggcta ttcaggcgct
     9961 gatgcgtttt tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct
    10021 taatgcattg ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt
    10081 attgctcaaa gccatcctgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt
    10141 tcttgtaccg cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg tgtggatgac
    10201 taaactcgaa tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct
    10261 cgaacctttt tgatggatta cgaaaatgat gaaaaaaagt attctggcgt ttctgttact
    10321 caccagttct gcagcggcgc tggcggctcc acaggtgata accgtgagtc gttttgaagt
    10381 gggtaaagac aaatgggcgt ttaatcgcga agaggtaatg ctgacttgcc gaccgggtaa
    10441 tgctttgtat gtcatcaacc caagtaccct cgtgcagtat cctttaaacg atatcgcaca
    10501 aaaggaagtt gccagtggga agaccaatgc ccaacccatt tcggtgattc agattgatga
    10561 tcctaacaat cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact
    10621 ctgttaatta cctaaaatag ccttttgatt tccaataaaa aaaccgcctc agttctttca
    10681 ccagaacggg cggtttttaa catttaagct gatgagcacc gcgcttttta ttgaccattt
    10741 tgcacgcaaa ctggaaaacc tggcgtcgtc atctattctt aaagggcaag gcaactaagc
    10801 ctgcattaat gccaactttt agcgcacggc tctctcccaa gagccatttc cctggaccga
    10861 atacaggaat cgtattcggt ctttttttaa ttgtatttaa aatcaattag ttgcaaacgt
    10921 ctccccgaaa ttccccgaaa tttactcgaa tttctgtatt ccggtctttt ttggttatat
    10981 cacaaccaaa atacatttaa caatccattt acgttaaaat cagagcagta agtacgtttt
    11041 ttctctctca tcaagataca tttttgttgt cttctccgat gtgtggccaa gtagacgctg
    11101 agcaaattct tctccacata tttctttgta caatcgtcca gccaggcttc tgatttcgtg
    11161 aaaagttggt gggttttcac tgaacaggat acctgttaat tttctggctg cgacaaattt
    11221 ctttgtcagg ccgtccgggt gaatgctgcc gtcagggctg ttttttctaa tcccggcact
    11281 gattagataa tcccccctac ttaccatgcg gcactgttca actactgcac caagtcgtaa
    11341 accagcgaca ggaaggctga gtgacagggg gatagcaatc atcattcctg tcttaatttg
    11401 cctgatgtgg agacgatcat cataaatatc actgaaccgc atattcgtta tgtcttcgcg
    11461 acgttgtcct gttacaaggg ctaagtccat agctaatggg aaccatgccg gaagttgatc
    11521 tgctgcctcc ctgatgcagt tgtatgtctt tagtttcagc cgttctcttg taactactat
    11581 tttcggtgct cttgttggcg ttactggatt ttgagatata cgtccttcaa caatggcttc
    11641 gcggaacata tcagataaca cagaacgcat tgatcctgcc atcgtgtttt tccctccttc
    11701 aatctacaaa tcaagaaact cggcgatatg gcgagtggtt atttctgtca gtaaaacccc
    11761 tcctaatttt tcttttattg tctccagttg atttacccga gttttataag tatttctgga
    11821 cgcttttctc cttataagaa tcgttttgta acgttcaatc cagcctgcca tagtaaatga
    11881 gtcgacccct ttaagctttt caattagggc agcaggagag tagtttttgt atatataatg
    11941 atttgcttca attgcctgcg ctactgcatc tcttcttgaa attttaccta gtgtaaattc
    12001 ttctttcgtc agagggttgc gccagtaata tgctttgtct ctccttcgat atgttaagtt
    12061 tctaggcaaa ttgggatcgt attttttccg ctgcatgttt taacttctcc attaacggac
    12121 tgtctctccc ttgtcgccca ttaggctgat ggtgtgttat atcggtatca accttatttg
    12181 ggttgatata gaaagcctcc ggaaccaccc tgtaactcct cccgtgtagt tcaggtgcag
    12241 gataaatgtt tccattcctt gcccatcgtc tcagcgttga tattgatggt gggttatccg
    12301 gatatctgag ttttccccac gttttgagtg tcacaagatt cattgccata cctctcacga
    12361 tatgaccgcc agtaaatata cagaatactg gcgggtgtgg ttgattttta ata
//
LOCUS       DADLDT020000115        12221 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000115 DADLDT020000000
VERSION     DADLDT020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.115"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..494)
                     /locus_tag="JQH22_003264"
     CDS             complement(<1..494)
                     /locus_tag="JQH22_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2207494.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JQH22_003265"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JQH22_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFB2207495.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="JQH22_003266"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="JQH22_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFB2207496.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2460..3326
                     /gene="folD"
                     /locus_tag="JQH22_003267"
     CDS             2460..3326
                     /gene="folD"
                     /locus_tag="JQH22_003267"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFB2207497.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3328..3540
                     /gene="ybcJ"
                     /locus_tag="JQH22_003268"
     CDS             3328..3540
                     /gene="ybcJ"
                     /locus_tag="JQH22_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFB2207498.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3648..4157
                     /locus_tag="JQH22_003269"
     CDS             3648..4157
                     /locus_tag="JQH22_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFB2207499.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="JQH22_003270"
     CDS             complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="JQH22_003270"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFB2207500.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5752..6246
                     /gene="ppiB"
                     /locus_tag="JQH22_003271"
     CDS             5752..6246
                     /gene="ppiB"
                     /locus_tag="JQH22_003271"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFB2207501.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6249..6971
                     /gene="lpxH"
                     /locus_tag="JQH22_003272"
     CDS             6249..6971
                     /gene="lpxH"
                     /locus_tag="JQH22_003272"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFB2207502.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7089..7598
                     /gene="purE"
                     /locus_tag="JQH22_003273"
     CDS             7089..7598
                     /gene="purE"
                     /locus_tag="JQH22_003273"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFB2207503.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7595..8662
                     /gene="purK"
                     /locus_tag="JQH22_003274"
     CDS             7595..8662
                     /gene="purK"
                     /locus_tag="JQH22_003274"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFB2207504.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYAYGVIWAQSKFS"
     gene            complement(8799..9692)
                     /locus_tag="JQH22_003275"
     CDS             complement(8799..9692)
                     /locus_tag="JQH22_003275"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFB2207505.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9689..10336)
                     /locus_tag="JQH22_003276"
                     /pseudo
     CDS             complement(9689..10336)
                     /locus_tag="JQH22_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10339..11571
                     /gene="allC"
                     /locus_tag="JQH22_003277"
     CDS             10339..11571
                     /gene="allC"
                     /locus_tag="JQH22_003277"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFB2207506.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11582..>12221
                     /gene="allE"
                     /locus_tag="JQH22_003278"
     CDS             11582..>12221
                     /gene="allE"
                     /locus_tag="JQH22_003278"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFB2207507.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2970 a         3156 c         3116 g         2979 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatgcaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caatctcatt tatcgactcc acatccgtat ataaccgatt actttattta
     2041 agacactgat agtagtaaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg
     2101 cactctattc taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc
     2161 aacacaaata tagacctgaa gcggtaaatt accaggctga aaattctttt tatattgtca
     2221 ggtatttctt aaattatctt aatccttaaa caaggaaata aatcagttcc agatttacaa
     2281 cgccatcatg tacgaaaaat gaagctttca atctcagcga cctcagcgac ggtgcgccta
     2341 accttcgcaa gaggtcgctt cacgcgataa atctgaaacg aaacctgaca gcgcgccccg
     2401 cttctgacaa aatagacgca tccccttcga cctacgtaac agatggaatc ctctctctga
     2461 tggcagcaaa gattattgac ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc
     2521 aaaaagttca ggctcgtatt gcagccggac tgcgggcacc aggactggcc gttgtgctgg
     2581 tgggtagtaa ccctgcatcg caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag
     2641 tcgggttcgt ctcccgctct tatgacctcc cggaaaccac cagcgaagcg gagctgctgg
     2701 agcttatcga tgcgctgaat gccgacaaca ccatcgatgg cattctggtt caactgccgt
     2761 taccggcggg tattgataac gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg
     2821 acggtttcca tccttacaac gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct
     2881 gcaccccgcg cggtatcgtc acgctgcttg agcgttacaa cattgatacc ttcggcctca
     2941 acgccgtggt gattggcgca tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc
     3001 tcgcaggttg caccaccacc gtgactcacc gcttcactaa aaatctgcgt catcacgtag
     3061 aaaatgccga tctgttgatc gttgccgttg gcaagccagg ctttattccc ggtgactgga
     3121 tcaaagaagg cgcaattgtg attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg
     3181 tgggcgacgt cgtgtttgaa gacgcggcta aacgcgcctc atacattacg cctgttcccg
     3241 gcggcgttgg cccgatgacg gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg
     3301 aatatcatga tccacagggt gagtaacatg gcgacatttt ctttaggtaa acatccgcac
     3361 gttgagctgt gcgacttgct gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa
     3421 atcgcgattg ccgaaggcca ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc
     3481 aaaatcgtcg ccggtcagac agtgagtttt gcaggacaca gcgtacaggt tgttgcctga
     3541 tccctttccc ggcctgtatc aacaggccgg ttctcttttc agacttttgc cctctacgcc
     3601 ccgttcatcc atgctgatag catcgacatt ttgccgaagc gaccatcatg ccaaccgtta
     3661 ttacgcacgc cgccgttccc ctttgcattg gtttagggtt gggttcgaaa ggtctgttat
     3721 ttgccggaat catcctggcg atgctgcccg atgccgacgt attgtcgttt aaatttggcg
     3781 ttgcttacgg caatgttttt ggtcatcgcg gttttaccca ttcgctggtg tttgcgtttg
     3841 ttgtcccgct cttatgtgtg ctgattggac gtcgatggtt cagggctggg ctgattcgct
     3901 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt actggattcg gtaaccactg
     3961 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
     4021 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacagcgtac gggcatcagg
     4081 tgattatttc cgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt
     4141 ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaattgcgc aattacttac
     4201 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
     4261 cacgtgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
     4321 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
     4381 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
     4441 ttgcattcgc cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa
     4501 acagcacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
     4561 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
     4621 agaggcgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagctggc
     4681 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
     4741 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
     4801 ccattccgga gtgcatccag tagttcacat actgaccatc atgggcacag gtggactgcg
     4861 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
     4921 ggttacccag ctgcttgcag ttcatggccg aacattcaat gtgccagcca ggacgacccg
     4981 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
     5041 ccatcgggtt gcgtttgtca tcaaccacgt caacgcgcgc gccagcctgc agctggtcga
     5101 gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc
     5161 cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
     5221 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
     5281 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
     5341 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
     5401 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
     5461 gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg
     5521 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
     5581 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
     5641 cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat
     5701 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
     5761 ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca
     5821 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
     5881 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
     5941 accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg
     6001 gcaatggcac gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
     6061 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
     6121 gctgaagtgg ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
     6181 aacggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
     6241 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
     6301 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata
     6361 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
     6421 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
     6481 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
     6541 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt aattatgcat ggcgacacgc
     6601 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
     6661 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg
     6721 cgaacagcaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
     6781 ctgtggtcag tgcgatggaa aaacatcagg tgcaatggct aatccacggg catacccatc
     6841 gcccggcggt gcatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactgggtg
     6901 cctggcatac ggaaggttcg atggtgaaag tcacggctga tgacgttgag ctgattcatt
     6961 ttccgtttta aaaaacccac aactttgctg atttcacagc cacgcaaccg ttttccttgc
     7021 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
     7081 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
     7141 gcgactgggc taccatgcag ttcgccgtcg aaatcttcga aatcctgaat gtcccgcacc
     7201 acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg
     7261 ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag
     7321 gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac
     7381 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
     7441 cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg
     7501 cgactcacga taaagagcta caccagcgtc tgaatggctg gcgcaaagcc cagaccgacg
     7561 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
     7621 cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc
     7681 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
     7741 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
     7801 gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaaacagc ttttcgataa
     7861 gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
     7921 gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
     7981 cggtcaatgg agtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggtga
     8041 atgtattgtc gagcagggca ttaacttctc tggtgaagta tcgctggttg gcgcgcgcgg
     8101 ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
     8161 gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat
     8221 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
     8281 caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg
     8341 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
     8401 gccgttaccg caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga
     8461 tgtgaattat gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt
     8521 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
     8581 tgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgcctacg gcgtgatttg
     8641 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
     8701 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
     8761 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
     8821 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta
     8881 cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg
     8941 gcaaatggag ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc
     9001 cagttttcat ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc
     9061 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
     9121 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
     9181 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
     9241 aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc ctcttgttct
     9301 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
     9361 gaaacctcaa tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag
     9421 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagtac atccagcgga
     9481 tacggttcta cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg
     9541 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca
     9601 ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg
     9661 ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa
     9721 gccagaaccc cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa
     9781 tcgcaacagc ggtacgtttc gggcaactca gagcatggac aaagtgcagg agtggcgagg
     9841 cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt
     9901 gaagattgtc ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat
     9961 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc
    10021 ctttacccag ccagtaccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    10081 gacgaaactg gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggcccga
    10141 aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag
    10201 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    10261 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    10321 cgaattgcgt gcggcgaaat tacacattcc cgtcaagcta tagaagaaac gctgccctgg
    10381 ctttcctctt ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcgccggaa
    10441 tggctggaaa cccagcagca atttaaaaaa agaatggcag caagtgggct ggaaacacgt
    10501 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    10561 gttctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    10621 ggcgcgctgg cggcgtggct ggcaattgac tggttgaaaa cgcaatacgg cgcaccgtta
    10681 cgtacggtcg aagtggtggc gatggcagaa gaagaaggca gccgcttccc gtatgtcttc
    10741 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    10801 gccaaaggaa atagttttgc cgatgcgatg aaggcttgcg gatttactct gccgaacgcc
    10861 ccactaactc cgcgtcaggc tataaaagcc tttgttgaac tgcatattga acagggctgt
    10921 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcagcgtcgc
    10981 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    11041 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    11101 atgggcgatc cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat
    11161 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg
    11221 cgcgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    11281 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc
    11341 gccaccctga cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt
    11401 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
    11461 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa
    11521 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcagaaata aggagtcata
    11581 aatgggatat ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat
    11641 tgttaaacac ggtaatttcg cactgttaac cccggatggt ctgataaaaa atattattcc
    11701 ggactttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    11761 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
    11821 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    11881 atttgcctta agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt
    11941 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga
    12001 aggccatgca ccgtggctgt tttctggcaa tgccagcgaa ctggaacgca ttcactatga
    12061 aggtatggat gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa
    12121 catgcatatc ctctcttttg caccaggtgc cagccacggt tatatcgaaa cacacgttca
    12181 ggaacatggt gcctatattc tttccggtca gggggtttat a
//
LOCUS       DADLDT020000116        12152 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000116 DADLDT020000000
VERSION     DADLDT020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.116"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            126..995
                     /locus_tag="JQH22_003279"
     CDS             126..995
                     /locus_tag="JQH22_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFB2207508.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="JQH22_003280"
     CDS             complement(1198..1551)
                     /locus_tag="JQH22_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFB2207509.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQH22_003281"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQH22_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFB2207510.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="JQH22_003282"
     CDS             complement(3379..3672)
                     /locus_tag="JQH22_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFB2207511.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="JQH22_003283"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="JQH22_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFB2207512.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQH22_003284"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQH22_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HFB2207513.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="JQH22_003285"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="JQH22_003285"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFB2207514.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="JQH22_003286"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="JQH22_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFB2207515.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="JQH22_003287"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="JQH22_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFB2207516.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="JQH22_003288"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="JQH22_003288"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFB2207517.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="JQH22_003289"
     CDS             11052..11627
                     /locus_tag="JQH22_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFB2207518.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="JQH22_003290"
     tRNA            11734..11809
                     /locus_tag="JQH22_003290"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2981 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DADLDT020000117        12061 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000117 DADLDT020000000
VERSION     DADLDT020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.118"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JQH22_003291"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JQH22_003291"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQH22_003292"
                     /pseudo
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQH22_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQH22_003293"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQH22_003293"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFB2207519.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQH22_003294"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQH22_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="JQH22_003295"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="JQH22_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFB2207520.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="JQH22_003296"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="JQH22_003296"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFB2207521.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JQH22_003297"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JQH22_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFB2207522.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="JQH22_003298"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="JQH22_003298"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFB2207523.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="JQH22_003299"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="JQH22_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFB2207524.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="JQH22_003300"
     CDS             9553..10332
                     /locus_tag="JQH22_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFB2207525.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="JQH22_003301"
     CDS             10322..11131
                     /locus_tag="JQH22_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HFB2207526.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="JQH22_003302"
     CDS             11218..11652
                     /locus_tag="JQH22_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFB2207527.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12061
                     /locus_tag="JQH22_003303"
     CDS             11649..>12061
                     /locus_tag="JQH22_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFB2207528.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3250 c         3073 g         2859 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag caccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 acaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga caccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgagaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaatc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcagttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggtgggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 c
//
LOCUS       DADLDT020000118        11922 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000118 DADLDT020000000
VERSION     DADLDT020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.119"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..2339)
                     /locus_tag="JQH22_003304"
     CDS             complement(<1..2339)
                     /locus_tag="JQH22_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFB2207529.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2273..2896)
                     /locus_tag="JQH22_003305"
     CDS             complement(2273..2896)
                     /locus_tag="JQH22_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFB2207530.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2893..4581)
                     /locus_tag="JQH22_003306"
                     /pseudo
     CDS             complement(2893..4581)
                     /locus_tag="JQH22_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JQH22_003307"
     CDS             complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JQH22_003307"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFB2207531.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7504..8226
                     /locus_tag="JQH22_003308"
     CDS             7504..8226
                     /locus_tag="JQH22_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HFB2207532.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8354..10285)
                     /gene="yfaL"
                     /locus_tag="JQH22_003309"
     CDS             complement(8354..10285)
                     /gene="yfaL"
                     /locus_tag="JQH22_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFB2207533.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10298..11893)
                     /locus_tag="JQH22_003310"
     CDS             complement(10298..11893)
                     /locus_tag="JQH22_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HFB2207534.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2946 g         2912 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggtac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc
      541 aaccattcat acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca
      601 accgactcgc cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg
      661 cgttcaatga ggatctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag
      721 acggttaaca aatagcggct cggtttatcg gcggcgggga gatttaacgc cacatgaccg
      781 ctggcgtcgg acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc
      841 aaatcgttac cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt
      901 tttaccggct cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt
      961 ttgaactctt ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc
     1021 acgcgaaaac tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg
     1081 gcaacggcat tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg
     1141 acatcgacgg tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg
     1201 ctgacgatgg gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg
     1261 acacgatcgc ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag
     1321 atttcgcttt cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc
     1381 agaatgtatg aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc
     1441 accccgcggg tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct
     1501 tcaccctgtt ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc
     1561 gccacggtat cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg
     1621 acgaggtaca gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc
     1681 gagatgaaat tgctggatgc gccttccagt tttacccctt gctgcggctc gaccggttta
     1741 gcctgccata gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac
     1801 tggttgttct gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt
     1861 aatgcctgag tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga
     1921 gatttgccgt accagttatc ccacagccag gtcagcgtat tgttcagccc gtcgcccaga
     1981 tattgcggtt gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg
     2041 tcaggaatac gatataggcg aacgtccacg ccgccgtact cttccatctg atagcgccga
     2101 taatcacgcc ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg
     2161 tcagcaagca aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa
     2221 tcatcagctt tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg
     2281 ccagaaaatt taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc
     2341 gggtgtcctt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
     2401 tcgtggcgct tccggtgtgg tagatgacgt aacgccccat ccagaccatt aagtgctggg
     2461 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa
     2521 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc
     2581 tcccctgatt ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg
     2641 gcaaatactg gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg
     2701 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
     2761 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt
     2821 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
     2881 gcagcccgtg tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg
     2941 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
     3001 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
     3061 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
     3121 gccatcagtg ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc
     3181 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
     3241 cacacggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     3301 ctgaccgtat cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg
     3361 gcttaccggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     3421 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     3481 ggtttgcaat tttgagtctt cataccagca cagccccgcg gcaccgtcta acgccccatt
     3541 cagcttgtcg ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     3601 cggcaccgcc acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc
     3661 gaaactggca tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     3721 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     3781 cagcaacctg gcgctgacca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc
     3841 ttccaggcca aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc
     3901 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag
     3961 catcttgtcc tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     4021 aacaggaata gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactagct
     4081 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     4141 cagcaccatg taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg
     4201 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     4261 gcggcgaatg ctgccttcaa tgcccagacg atcggcatga ttctgataat agaaaacgaa
     4321 atcttcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     4381 cagagagtcg ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     4441 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     4501 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     4561 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     4621 cacagccaaa ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt
     4681 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     4741 ctggctcgtc gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc
     4801 cgtcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta
     4861 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc
     4921 tgatttctga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     4981 cgcagtcatc tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg
     5041 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac
     5101 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     5161 cgactttatc gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg
     5221 cacggacaga agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt
     5281 catcttcgcc actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     5341 ccactttacc ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac
     5401 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg
     5461 cggtgatacg ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc
     5521 cacgagtggc ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga
     5581 agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     5641 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     5701 gctgatattt aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca
     5761 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     5821 actgttcacg aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac
     5881 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     5941 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     6001 gttcggtcag gtagtacaga ccatcacgca cgccgaactc aggctccagc cattccggac
     6061 gcgcagcatc gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat
     6121 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     6181 tcgggtcaat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     6241 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     6301 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     6361 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     6421 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     6481 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     6541 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt
     6601 tggcgtcaac ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt
     6661 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     6721 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     6781 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     6841 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     6901 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     6961 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     7021 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     7081 ccatacggac gatcgtgtca taaaccgccg agtcaccatg gggatggtat ttaccgatta
     7141 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     7201 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     7261 cacggccaac aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa
     7321 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     7381 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     7441 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     7501 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accacgaaga gatcgctaaa
     7561 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     7621 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     7681 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     7741 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg
     7801 gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     7861 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag
     7921 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     7981 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     8041 atggtgccca aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc
     8101 tgggtggatc agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc
     8161 actaattcct ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     8221 aagtgaggtt gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag
     8281 gcctacaaat gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat
     8341 ggtcagacag cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     8401 catcactccc tttctgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc
     8461 ccgtgactcc cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc
     8521 catcttcttc aatttccgtc gcatggggat cgtgatagta attcagatct aatgttggtg
     8581 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     8641 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     8701 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     8761 cgacttccag cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt
     8821 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     8881 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     8941 tatcggcgcg agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac
     9001 caaccgcccc aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc
     9061 taagctgcac cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat
     9121 aacgtccgcc gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc
     9181 gctccatcat aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg
     9241 cattcagcac cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg
     9301 gcgtgggatc aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt
     9361 gcgatcgcag ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt
     9421 tgacgtagcc actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag
     9481 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     9541 taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg
     9601 agtcatcgcc gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt
     9661 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     9721 tcagatcacc attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg
     9781 tattaagctc gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt
     9841 taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
     9901 catcgctgat gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg
     9961 cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat
    10021 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
    10081 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    10141 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    10201 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    10261 tatcgacatt ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct
    10321 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    10381 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    10441 catcgaccac cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac
    10501 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    10561 ggccatcatt gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac
    10621 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
    10681 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    10741 gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    10801 tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag
    10861 tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta
    10921 gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg
    10981 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
    11041 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    11101 gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt
    11161 cattctctgt attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    11221 ttaagccgag atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg
    11281 cgctattgtt ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    11341 tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg
    11401 ctgtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
    11461 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    11521 tatatgcctg gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat
    11581 aaattgcgcc acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat
    11641 cgacggcacc agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga
    11701 cgttaccgga aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg
    11761 ccccgccgtt attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt
    11821 cattttctgg taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg
    11881 cattattggt catatccgaa aaaaccagcc attgcccatc gg
//
LOCUS       DADLDT020000119        11679 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000119 DADLDT020000000
VERSION     DADLDT020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.120"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            404..2044
                     /gene="trg"
                     /locus_tag="JQH22_003311"
     CDS             404..2044
                     /gene="trg"
                     /locus_tag="JQH22_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFB2207535.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2082..3005)
                     /locus_tag="JQH22_003312"
     CDS             complement(2082..3005)
                     /locus_tag="JQH22_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2207536.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3222..4565
                     /locus_tag="JQH22_003313"
     CDS             3222..4565
                     /locus_tag="JQH22_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFB2207537.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4790..6445
                     /gene="opgD"
                     /locus_tag="JQH22_003314"
                     /gene_synonym="mdoD"
     CDS             4790..6445
                     /gene="opgD"
                     /locus_tag="JQH22_003314"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFB2207538.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6585..6809
                     /locus_tag="JQH22_003315"
     CDS             6585..6809
                     /locus_tag="JQH22_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFB2207539.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6872..7408
                     /gene="rimL"
                     /locus_tag="JQH22_003316"
     CDS             6872..7408
                     /gene="rimL"
                     /locus_tag="JQH22_003316"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFB2207540.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7403..8383)
                     /locus_tag="JQH22_003317"
     CDS             complement(7403..8383)
                     /locus_tag="JQH22_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207541.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8507..9499
                     /gene="tehA"
                     /locus_tag="JQH22_003318"
     CDS             8507..9499
                     /gene="tehA"
                     /locus_tag="JQH22_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFB2207542.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9496..10089
                     /gene="tehB"
                     /locus_tag="JQH22_003319"
     CDS             9496..10089
                     /gene="tehB"
                     /locus_tag="JQH22_003319"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFB2207543.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10392..11060
                     /locus_tag="JQH22_003320"
     CDS             10392..11060
                     /locus_tag="JQH22_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFB2207544.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11078..11584)
                     /gene="tnpA"
                     /locus_tag="JQH22_003321"
                     /pseudo
     CDS             complement(11078..11584)
                     /gene="tnpA"
                     /locus_tag="JQH22_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11592..>11679
                     /locus_tag="JQH22_003322"
     CDS             11592..>11679
                     /locus_tag="JQH22_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2207545.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACR"
BASE COUNT         3020 a         2717 c         2929 g         3013 t
ORIGIN      
        1 tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat cgtccaacaa
       61 aatgggtgca gtacagttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
      121 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
      181 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcgggtt
      241 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
      301 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
      361 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
      421 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
      481 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
      541 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
      601 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
      661 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
      721 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt
      781 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
      841 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
      901 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
      961 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     1021 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     1081 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     1141 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     1201 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     1261 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     1321 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     1381 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     1441 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     1501 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     1561 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     1621 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga
     1681 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     1741 agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     1801 aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc
     1861 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc
     1921 ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa
     1981 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     2041 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     2101 cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt
     2161 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     2221 cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta
     2281 agttgacgag atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc
     2341 tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     2401 accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     2461 cggacaacca gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     2521 tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga
     2581 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     2641 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg
     2701 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     2761 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     2821 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag
     2881 agcgcaggtt gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg
     2941 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     3001 tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga
     3061 taacttgatt gcaaaatatt attaataatt aaagcaatta tgttacagca aaatggataa
     3121 tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg
     3181 ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg
     3241 cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc
     3301 cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca
     3361 atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac
     3421 gtcatggggc gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg
     3481 ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac
     3541 attcgacagc atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt
     3601 ttacatcctt actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg
     3661 agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg
     3721 agcagcaggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa
     3781 cgtttcgctg gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg
     3841 aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc
     3901 cacgtacgct ggatattgac cgggtgcagt cgatgacgcc tgaatgcgga attgaaccca
     3961 aaattctcat cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct
     4021 ttaaagcact ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc
     4081 gctttggtga aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg
     4141 atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac
     4201 aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat
     4261 ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag
     4321 acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag
     4381 atttcttgcc cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga
     4441 cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt
     4501 tggatgagtt ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc
     4561 tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct
     4621 catcccatcg acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga
     4681 ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa
     4741 acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag
     4801 acgatttatt aaaggtttaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc
     4861 tcttttttct caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg
     4921 ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg
     4981 tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat
     5041 tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca
     5101 gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc
     5161 aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg
     5221 tgttgatacc aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt
     5281 taaagccccc gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg
     5341 cgccgttgat gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta
     5401 caccgacagt aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc
     5461 gggggcaact acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta
     5521 taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta
     5581 tgcgcgcaaa gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg
     5641 tactaatgaa cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct
     5701 gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt
     5761 gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg
     5821 tgacttctcc cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt
     5881 ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg
     5941 cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga
     6001 tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt
     6061 agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg
     6121 tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct
     6181 gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa
     6241 gcaaatcgaa attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta
     6301 tccgacttcg gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga
     6361 cgctatcagt gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta
     6421 tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc
     6481 gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt
     6541 gtgtatttca gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc
     6601 gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac
     6661 acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg
     6721 cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc
     6781 tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt
     6841 ttaacaacag catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc
     6901 acttgaatta catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa
     6961 aaataaaacc tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga
     7021 cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt
     7081 catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact
     7141 gaataaaact gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat
     7201 ttctcaggcg ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt
     7261 cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt
     7321 tatccttgaa ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa
     7381 cttatacgcg cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct
     7441 cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa
     7501 cgacggcgtg gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc
     7561 tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat
     7621 ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg
     7681 ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct
     7741 gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt
     7801 caacactggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg
     7861 catcgccata aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat
     7921 aaatttggag caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat
     7981 gttgatcaat taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa
     8041 tggtgacact gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt
     8101 tatccgttgc gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg
     8161 aaatcaccgt tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct
     8221 gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa
     8281 aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat
     8341 aactaaaggc tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt
     8401 gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa
     8461 atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa
     8521 agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt
     8581 tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt
     8641 gattctggcg atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg
     8701 ctttccgcat agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt
     8761 gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc
     8821 ggtgtgcatg ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc
     8881 gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac
     8941 agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg
     9001 tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca
     9061 gcgtctgcgc agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct
     9121 cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac
     9181 gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat
     9241 gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc
     9301 tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac
     9361 gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac
     9421 ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa
     9481 agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta
     9541 acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg
     9601 gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt
     9661 gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa
     9721 aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag
     9781 tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg
     9841 ttgattgcca atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg
     9901 atggataccg ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa
     9961 ttacgtcgat attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg
    10021 caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt
    10081 aaaaaatgac ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg
    10141 ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg
    10201 aagtttttta gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt
    10261 ttcggcattg actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt
    10321 tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca
    10381 ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg
    10441 ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atattctggt tttttaaaca
    10501 attactctga tttaaaagaa acaacctcag caacaggtaa acctgtttta cgttgggtag
    10561 acccgagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc acttattatc
    10621 cggtaccaaa accttctacc caggtagggc agaaagttct ggataaaatt ttgaactata
    10681 ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc agtggttacc accgctgggc
    10741 cgcgtagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa gaagggctgc
    10801 aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg gctacaggcc
    10861 accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca gcgactaata
    10921 aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc
    10981 caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat gccaccatgt
    11041 ttgacgttaa caaaaagtag ttccagacgc gccatcgtta gatggcgctt tttatccggt
    11101 gcgccgtaaa accccatcct tcagggcggg gatataaggc gcggttttcc acctgaccag
    11161 gtgtttgctg ttgttcaatg tattggcgga tgacggatat tggcgcacct ccgcagctac
    11221 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
    11281 gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata
    11341 tcgccagttt gggaggttag ttaattaaca aatggacgtg atctggttcg ccatccattt
    11401 caaccagttc agcttcaaaa taagcacata catttgaaaa gtaagtgcgt agtttttctg
    11461 tcgcgtcatg gtcaaaaatc tggcgtcggt atctggtgac aaagactagg tgaacatgca
    11521 tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta
    11581 tcatttgacc tgtgaaacga ttacaggcat ttaaattcca gttaagacca ggtggtcaac
    11641 aggagtgtga aatgaggcgc ttcgcaggcg catgtcgtt
//
LOCUS       DADLDT020000120        11678 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000120 DADLDT020000000
VERSION     DADLDT020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.121"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            597..794
                     /locus_tag="JQH22_003323"
     CDS             597..794
                     /locus_tag="JQH22_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207546.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JQH22_003324"
     CDS             complement(744..1937)
                     /locus_tag="JQH22_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207547.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JQH22_003325"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JQH22_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFB2207548.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JQH22_003326"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JQH22_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HFB2207549.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JQH22_003327"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JQH22_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HFB2207550.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="JQH22_003328"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="JQH22_003328"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HFB2207551.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="JQH22_003329"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="JQH22_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFB2207552.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="JQH22_003330"
     CDS             complement(10573..10716)
                     /locus_tag="JQH22_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207553.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10977..11678
                     /locus_tag="JQH22_003331"
                     /pseudo
     CDS             10977..11678
                     /locus_tag="JQH22_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2965 a         2901 c         2994 g         2818 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta acgatcaatt agtattgatt cacataatct ctcatcatta atgagaacat
    10861 tgtcacatat ttacaatata gaactgttac ttttagcaag ctgttagtaa ccaaccgtta
    10921 ctaataattt taattcttaa tactttgttc attcaataaa tgagttgaga gaatatatga
    10981 ataaaatata ctcccttaaa tatagtgctg ccactggcgg actcattgct gtttctgaat
    11041 tagcgaaaag agtttctggt aaaacaaacc gaaaacttgt agcaacaatg ttgtctctgg
    11101 ctgttgcagg tacagtaaat gcagcaaata ttgatatatc aaatgtatgg gcgagagact
    11161 atcttgatct tgcacaaaat aaaggtattt tccagcccgg agcaacagac gtaacaatca
    11221 ctttaaaaaa cggagataaa ttctctttcc ataatctctc aattccggat ttttctggtg
    11281 cagcagcgag tggcgcagct accgcaataa gaggttctta tagtgttact gttgcacata
    11341 acaaaaagaa ccctcaggcc gcagaaactc aggtttacgc tcagtcttct tacaaggttg
    11401 ttgacagaag aaattccaat gattttgaga ttcagaggtt aaataaattt gttgtggaaa
    11461 cagtaggtgc caccccggca gagaccaacc ctacaacata ttctgatgca ttagaacgct
    11521 acggtatagt cacttctgac ggttcaaaaa aatcataggt tttcgtgctg gctctggagg
    11581 aacatcattt attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct
    11641 gttaagtgct agtctatttg aggtcaccca atgggact
//
LOCUS       DADLDT020000121        11566 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000121 DADLDT020000000
VERSION     DADLDT020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.122"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(217..292)
                     /locus_tag="JQH22_003332"
     tRNA            complement(217..292)
                     /locus_tag="JQH22_003332"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="JQH22_003333"
     CDS             851..3103
                     /locus_tag="JQH22_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207554.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4416
                     /gene="csiD"
                     /locus_tag="JQH22_003334"
     CDS             3439..4416
                     /gene="csiD"
                     /locus_tag="JQH22_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HFB2207555.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4436..5704
                     /gene="lhgO"
                     /locus_tag="JQH22_003335"
     CDS             4436..5704
                     /gene="lhgO"
                     /locus_tag="JQH22_003335"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFB2207556.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5727..7175
                     /gene="gabD"
                     /locus_tag="JQH22_003336"
                     /pseudo
     CDS             5727..7175
                     /gene="gabD"
                     /locus_tag="JQH22_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7183..8469
                     /gene="gabT"
                     /locus_tag="JQH22_003337"
     CDS             7183..8469
                     /gene="gabT"
                     /locus_tag="JQH22_003337"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFB2207557.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8707..10107
                     /gene="gabP"
                     /locus_tag="JQH22_003338"
     CDS             8707..10107
                     /gene="gabP"
                     /locus_tag="JQH22_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFB2207558.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10128..10790
                     /gene="csiR"
                     /locus_tag="JQH22_003339"
     CDS             10128..10790
                     /gene="csiR"
                     /locus_tag="JQH22_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFB2207559.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10791..11240)
                     /gene="kbp"
                     /locus_tag="JQH22_003340"
     CDS             complement(10791..11240)
                     /gene="kbp"
                     /locus_tag="JQH22_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFB2207560.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11324..11482)
                     /locus_tag="JQH22_003341"
     CDS             complement(11324..11482)
                     /locus_tag="JQH22_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFB2207561.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2973 a         2840 c         2885 g         2868 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 gagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggtccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgagca aaacatgcag ggtggaaatt cgttgctgct gcatctcgat
     4021 gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc gatgcgcttt
     4081 gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat
     4141 caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa agattttgaa
     4201 gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg cattctttct
     4261 gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct gcacggtcgc
     4321 gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc
     4381 gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg agcggatgta
     4441 tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga
     4501 tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca
     4561 gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg gcagcctgaa
     4621 ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc aaaacggcat
     4681 tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa tggaacggat
     4741 gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga taaacgccgt
     4801 ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg tgccgtccag
     4861 cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc aggccagagg
     4921 cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa acggcgtggt
     4981 gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct gttccgggct
     5041 gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt
     5101 ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc acctgattta
     5161 ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca tgatcgacgg
     5221 cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg
     5281 cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc gccgggtgct
     5341 gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta
     5401 tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc
     5461 cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg attttctgtt
     5521 tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc
     5581 aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa
     5641 ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg catttaatca
     5701 ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta ttccgccagc
     5761 aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca
     5821 atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat gaaacccgcg
     5881 ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac
     5941 gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac gatttagcgc
     6001 gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg
     6061 ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc tacaccattc
     6121 ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc gtcaccgccg
     6181 ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt ccggcgctgg
     6241 cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct gcgctggcgc
     6301 tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt
     6361 cggcaggcgc ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta
     6421 ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag
     6481 tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata
     6541 aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg
     6601 ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttacagcagg
     6661 cggtgagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc gggccgctga
     6721 tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg gagaaaggtg
     6781 cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc cagccaacca
     6841 ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc ggccccctcg
     6901 ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat gacaccgagt
     6961 ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc gtcggcgagg
     7021 cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa gtggccccat
     7081 tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt
     7141 acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag gaacagcaat
     7201 aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca aattcacccg
     7261 attttcgctg accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt
     7321 gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc
     7381 gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag
     7441 ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt cgccaagaaa
     7501 acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc
     7561 gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg cacgcattac
     7621 accctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct gatgccgggt
     7681 catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga tgacgctatc
     7741 gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc cgccatcatg
     7801 attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt
     7861 ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc
     7921 gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc ggatcttacc
     7981 acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg gcgcgcggaa
     8041 gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa cccgattgcc
     8101 tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct gcaaaaagcc
     8161 aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa acacccggag
     8221 atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga agatggcgat
     8281 cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg cgataagggc
     8341 ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accactcacc
     8401 attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt tgatgaggcg
     8461 aagcagtagc accgccccca tgcaggaggc gacgctgcgc gtcttgtccg gcctacgaga
     8521 atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa caggaataaa
     8581 tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat aacaattaaa
     8641 cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga
     8701 ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa atcacgccac
     8761 gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc
     8821 gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg attactggtg
     8881 gttatgatta tgcggatgct ggcggaaatg gcagttgcta cgcccgatac cggttcgttt
     8941 tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg ctggttgtac
     9001 tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat gatcctgcac
     9061 tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct cgccttaact
     9121 ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc
     9181 aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac
     9241 ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac
     9301 ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa
     9361 attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt ccgcgccacc
     9421 aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca
     9481 ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa
     9541 ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc
     9601 agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc
     9661 ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg
     9721 ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc gcctgcgaag
     9781 gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc
     9841 gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga aattcgcttg
     9901 cgaatgtggc ttgatccctg gctcacctgg ctggtcatca gctttattac ctttgtgttg
     9961 gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg
    10021 atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa
    10081 aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt
    10141 ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg
    10201 aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg ccgttgcagg
    10261 aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc
    10321 gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc gccaatatgg
    10381 aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag gcagacgttc
    10441 tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg
    10501 atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc
    10561 actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat cgatttatct
    10621 ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat cagcaccaga
    10681 ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta atgcgccagc
    10741 atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc
    10801 ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc
    10861 gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc
    10921 agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc
    10981 acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc
    11041 ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg
    11101 ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc
    11161 tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc ggcatcttta
    11221 acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag
    11281 cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca
    11341 cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa
    11401 tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga atgatggtga
    11461 tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa
    11521 gagattttat ctcttaagcg tagtaatttt agcggagcct gccagt
//
LOCUS       DADLDT020000122        11069 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000122 DADLDT020000000
VERSION     DADLDT020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.123"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            50..1540
                     /locus_tag="JQH22_003342"
     CDS             50..1540
                     /locus_tag="JQH22_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFB2207562.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1592..2749
                     /locus_tag="JQH22_003343"
     CDS             1592..2749
                     /locus_tag="JQH22_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFB2207563.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2715..2837)
                     /locus_tag="JQH22_003344"
     CDS             complement(2715..2837)
                     /locus_tag="JQH22_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207564.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3040..4725
                     /locus_tag="JQH22_003345"
                     /pseudo
     CDS             3040..4725
                     /locus_tag="JQH22_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4763..7123
                     /locus_tag="JQH22_003346"
     CDS             4763..7123
                     /locus_tag="JQH22_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFB2207565.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7180..9963
                     /locus_tag="JQH22_003347"
     CDS             7180..9963
                     /locus_tag="JQH22_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HFB2207566.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10324..>11069
                     /locus_tag="JQH22_003348"
     CDS             10324..>11069
                     /locus_tag="JQH22_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFB2207567.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGG"
BASE COUNT         3248 a         2465 c         2427 g         2929 t
ORIGIN      
        1 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
       61 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      121 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      181 aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg gctatgtggc
      241 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      301 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      361 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      421 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      481 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      541 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      601 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      661 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctacc
      721 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
      781 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
      841 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
      901 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
      961 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1021 ttatgacaag ctgatttccg caatggattt ctacccaaca gctcttgatg cagccgatat
     1081 cagcattcca aaagacctta agctggatgg tgttcccttg ctgccctggt tgcaagataa
     1141 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1201 tgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
     1261 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1321 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1381 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1441 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1501 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1561 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1621 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1681 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgaagcgtc
     1741 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     1801 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     1861 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     1921 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
     1981 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2041 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt atcctcttga
     2101 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     2161 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2221 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2281 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     2341 ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2401 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2461 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2521 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2581 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2641 ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta agaacagagt
     2701 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     2761 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     2821 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     2881 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     2941 atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag taaatcattg
     3001 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     3061 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     3121 aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg cgtggttaaa
     3181 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     3241 accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat ctttgtatta
     3301 atatccgtaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3361 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3421 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3481 atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3541 tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg tggaacagaa
     3601 tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg tggaacttta
     3661 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     3721 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     3781 aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat taaagagaac
     3841 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat ttattcgcgc
     3901 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     3961 ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa tttaagctgg
     4021 tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     4081 ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca acatgcggta
     4141 caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga gaacttgaac
     4201 tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc gggaaaaacc
     4261 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     4321 gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact gaaagagatt
     4381 atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt actgcatcag
     4441 gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4501 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4561 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg cttactgcgt
     4621 ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4681 tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca ataaaagccc
     4741 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     4801 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat
     4861 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     4921 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     4981 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa
     5041 aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc
     5101 aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     5161 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     5221 ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg
     5281 cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt
     5341 aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac
     5401 ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc
     5461 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5521 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5581 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5641 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     5701 gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact
     5761 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     5821 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt
     5881 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     5941 attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     6001 tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa
     6061 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct
     6121 gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga
     6181 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     6241 ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat
     6301 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6361 aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt
     6421 gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag
     6481 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     6541 caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg
     6601 ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     6661 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     6721 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     6781 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     6841 agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     6901 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     6961 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     7021 caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc
     7081 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     7141 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     7201 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     7261 gatgaaaagt taattaccgg gcaactggac aatgacttgc gatatatgat ttatccgcat
     7321 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     7381 gaagacaatg agctcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
     7441 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     7501 gatgttaatg cctatatcag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     7561 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     7621 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     7681 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
     7741 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac gccggcacaa
     7801 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     7861 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     7921 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     7981 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
     8041 gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag
     8101 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     8161 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     8221 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     8281 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     8341 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     8401 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     8461 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     8521 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
     8581 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
     8641 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
     8701 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag
     8761 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
     8821 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     8881 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     8941 aactcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     9001 cggactaata accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg
     9061 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     9121 gaccagcgtc cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac
     9181 cgcacgaaat tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct
     9241 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     9301 gcagaagaca aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat
     9361 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
     9421 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     9481 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     9541 ctacgcgtta atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg
     9601 gttgatcctc aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     9661 catgatgaac agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc
     9721 agtgagcaag aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     9781 cgtagcgttc aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct
     9841 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     9901 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
     9961 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
    10021 gtaataattt tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg
    10081 cctgattttt acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa
    10141 cacctttcca ccacccaaga attctctatt acattgtttt taatgaaaaa atatcattcc
    10201 aacaatatct cacgataaat aacattagga ttttgttatt taaacacgag tcctttgcac
    10261 ttgcttactt tatcgataaa tcctactttt ttaatgcgat ccaatcattt taaggagttt
    10321 aaaatggata agaagcaagt aacggattta aggtcggaac tactcgattc acgttttggt
    10381 gcgaagtcta tttccactat cgcagaatca aaacgttttc cgctgcacga aatgcgcgac
    10441 gatgtcgcat ttcagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    10501 ctggccactt tctgccagac ctgggacgac gaaaacgtcc acaagttgat ggatttatcc
    10561 attaacaaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
    10621 tgcgtaaaca tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt
    10681 ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg
    10741 cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc
    10801 ggtccggtgc aaatctgctg gcataaattc gcccgctact gggatgtgga gctgcgtgag
    10861 atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga agcctgcgac
    10921 gaaaatacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa ctatgagttc
    10981 ccgcaaccgc tgcacgatgc actggataaa ttccaggccg acaccggtat cgacatcgac
    11041 atgcacatcg acgccgccag cggtggctt
//
LOCUS       DADLDT020000123        10928 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000123 DADLDT020000000
VERSION     DADLDT020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.124"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            156..506
                     /locus_tag="JQH22_003349"
     CDS             156..506
                     /locus_tag="JQH22_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFB2207568.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            559..954
                     /locus_tag="JQH22_003350"
     CDS             559..954
                     /locus_tag="JQH22_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFB2207569.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="JQH22_003351"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="JQH22_003351"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFB2207570.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="JQH22_003352"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="JQH22_003352"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFB2207571.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="JQH22_003353"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="JQH22_003353"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFB2207572.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="JQH22_003354"
     CDS             complement(5325..6425)
                     /locus_tag="JQH22_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFB2207573.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="JQH22_003355"
     CDS             complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="JQH22_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFB2207574.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7573..8139)
                     /locus_tag="JQH22_003356"
     CDS             complement(7573..8139)
                     /locus_tag="JQH22_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFB2207575.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8355..10088
                     /gene="argS"
                     /locus_tag="JQH22_003357"
     CDS             8355..10088
                     /gene="argS"
                     /locus_tag="JQH22_003357"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFB2207576.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10265..10753
                     /locus_tag="JQH22_003358"
     CDS             10265..10753
                     /locus_tag="JQH22_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFB2207577.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2671 a         2789 c         2605 g         2863 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt
      721 cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgtatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat
     6541 gatttattac aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatata
     6601 tatttatcag ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta
     6661 gcacaacgct atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat
     6721 actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca
     6781 tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt
     6841 cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt
     6901 tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct
     6961 cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca
     7021 cggactcctg ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt
     7081 tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc
     7141 aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga
     7201 aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca
     7261 ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg
     7321 cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt
     7381 gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt cagtaccccc
     7441 aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg
     7501 ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt
     7561 tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg
     7621 aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg
     7681 ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga
     7741 aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat
     7801 atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct ccacattgga
     7861 aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc
     7921 attgatcatt ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc
     7981 agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc
     8041 caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc
     8101 aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt
     8161 aaaagcgtta atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct
     8221 gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg
     8281 cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa
     8341 tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga
     8401 ttgcggcagg cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt
     8461 tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc
     8521 gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg
     8581 agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg
     8641 ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg
     8701 tggttgacta ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta
     8761 ccattattgg tgacacagca gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg
     8821 caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc
     8881 agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg
     8941 cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa
     9001 aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca
     9061 tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga
     9121 tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag
     9181 gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg
     9241 aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca
     9301 ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt
     9361 acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag
     9421 gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag
     9481 acggcaaacc gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac ctgctggatg
     9541 aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg
     9601 agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca
     9661 aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata
     9721 ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa
     9781 ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac
     9841 tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc
     9901 cgcatgtaat gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc
     9961 actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac
    10021 aactgacagc gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc
    10081 gtatgtaatc gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt
    10141 tattgccatc taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa
    10201 gaggccagaa gcgtaatatc caggcccagg aaaacgataa cggttcaatt taaggaatac
    10261 cgaagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga
    10321 agatacggtt atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa
    10381 ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatct
    10441 cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag aacaagtgcc
    10501 acgcacaggt gcaaaaaaac ttgatactga acaacttaac ttgcttaaaa aatcacagcg
    10561 agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg aggatgttca
    10621 ggatttaagt gacccctatt ctgaatcaga atggctatca tgtatgatca tacagaccaa
    10681 tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc caagcccttt
    10741 gacaagagga taattcacat ctttttggca tgttttgttg caagctattc ctgataaatg
    10801 attgcaacaa aacatccagc cttttgactg agttattaaa catactcgcg agcgcgtaat
    10861 tttttgtctt tcagcgataa ttcacaatca cttcattccg ttgtaccttt agcggtggaa
    10921 ttaaccgc
//
LOCUS       DADLDT020000124        10925 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000124 DADLDT020000000
VERSION     DADLDT020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.125"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..203
                     /locus_tag="JQH22_003359"
     CDS             <1..203
                     /locus_tag="JQH22_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207578.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="JQH22_003360"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="JQH22_003360"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFB2207579.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="JQH22_003361"
     CDS             2102..2818
                     /locus_tag="JQH22_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFB2207580.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="JQH22_003362"
     tRNA            complement(3274..3349)
                     /locus_tag="JQH22_003362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="JQH22_003363"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="JQH22_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="JQH22_003364"
     tRNA            5082..5157
                     /locus_tag="JQH22_003364"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="JQH22_003365"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="JQH22_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFB2207581.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="JQH22_003366"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="JQH22_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFB2207582.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="JQH22_003367"
     tRNA            7492..7567
                     /locus_tag="JQH22_003367"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="JQH22_003368"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="JQH22_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFB2207583.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="JQH22_003369"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="JQH22_003369"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFB2207584.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="JQH22_003370"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="JQH22_003370"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFB2207585.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="JQH22_003371"
     CDS             complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="JQH22_003371"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFB2207586.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2364 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaa
//
LOCUS       DADLDT020000125        10901 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000125 DADLDT020000000
VERSION     DADLDT020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.126"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="JQH22_003372"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="JQH22_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFB2207587.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="JQH22_003373"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="JQH22_003373"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFB2207588.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="JQH22_003374"
     CDS             complement(2271..3686)
                     /locus_tag="JQH22_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207589.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="JQH22_003375"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="JQH22_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFB2207590.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="JQH22_003376"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="JQH22_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="JQH22_003377"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="JQH22_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFB2207591.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DADLDT020000126        10673 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000126 DADLDT020000000
VERSION     DADLDT020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.127"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            60..755
                     /locus_tag="JQH22_003378"
     CDS             60..755
                     /locus_tag="JQH22_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2207592.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
     gene            834..1994
                     /locus_tag="JQH22_003379"
                     /pseudo
     CDS             834..1994
                     /locus_tag="JQH22_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            2040..3179
                     /locus_tag="JQH22_003380"
     CDS             2040..3179
                     /locus_tag="JQH22_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFB2207593.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            3167..3613
                     /gene="etp"
                     /locus_tag="JQH22_003381"
                     /gene_synonym="yccY"
     CDS             3167..3613
                     /gene="etp"
                     /locus_tag="JQH22_003381"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFB2207594.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            3633..5813
                     /gene="etk"
                     /locus_tag="JQH22_003382"
     CDS             3633..5813
                     /gene="etk"
                     /locus_tag="JQH22_003382"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFB2207595.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(5928..7226)
                     /gene="appA"
                     /locus_tag="JQH22_003383"
     CDS             complement(5928..7226)
                     /gene="appA"
                     /locus_tag="JQH22_003383"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFB2207596.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(7306..7398)
                     /gene="cbdX"
                     /locus_tag="JQH22_003384"
     CDS             complement(7306..7398)
                     /gene="cbdX"
                     /locus_tag="JQH22_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFB2207597.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(7410..8546)
                     /gene="appB"
                     /locus_tag="JQH22_003385"
     CDS             complement(7410..8546)
                     /gene="appB"
                     /locus_tag="JQH22_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFB2207598.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(8558..10102)
                     /gene="appC"
                     /locus_tag="JQH22_003386"
     CDS             complement(8558..10102)
                     /gene="appC"
                     /locus_tag="JQH22_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFB2207599.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(10236..>10673)
                     /locus_tag="JQH22_003387"
     CDS             complement(10236..>10673)
                     /locus_tag="JQH22_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFB2207600.1"
                     /translation="ALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRNPDAQ
                     PHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLK
                     GPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPM"
BASE COUNT         2804 a         2762 c         2724 g         2383 t
ORIGIN      
        1 tccgaagaaa attgccggac aaaccgctcg ctgacatatc aacgaaagag gtggcagcaa
       61 tgctaaacac ctacgtagca gaaggtaaag cggcttccgc aaaattaatc aggtcaaccc
      121 ttgttgacgt ttttcgtgag gcaatagccg aggggcatgt agcaacgaat ccggtaacag
      181 caacccgcac agcaaagtca gaagtaaggc gctcaaggct gacagctaat gagtatgtcg
      241 cgatttacca tgcagccgaa cctctcccaa tctggctgag gctagcaatg gatttggctg
      301 tcgttacagg gcagagagtc ggcgatttgt gcagaatgaa atggtcagac ataaacgaca
      361 accatcttca cattgaacag agcaaaacag gggctaagct cgccattccg ctgacgctaa
      421 cgattgacgc gctcaatatc tcattggctg atacactaca gaaatgcagg gaggccagca
      481 gcagtgaaac tataatcgca tcaaagcatc acgatccgct ttccccgaaa acagtatcga
      541 agtattttac aaaggcgaga aatgcatcag gactctcatt tgatggagac ccgccaacat
      601 tccatgaact gcgtagcctg tcggcgaggc tataccgtaa tcagattggc gataagtttg
      661 ctcaacgtct tctcggacat aaatcagatt caatggcggc gcggtatagg gacagccgtg
      721 gacgggaatg ggacaaaatt gaaatcgaca aatgatttta ttttgactaa taatgacctt
      781 cttatactaa ttcattgata gtcaaagaga ttttaaatat acaacttatt cacctgaagt
      841 ataacgcggg ctttgacgcg ccagaaattc agctacgcga taagacgctg tatatgtctg
      901 gtcagcaata caaataccgt gactctcgcg aagcggtcga tcgtgccaac cggattctgg
      961 tgaataacct gccgcaaggc gttgagaaga ttagcgtgac gcaaaagcgc gagcatatgg
     1021 cgatggtgac taccgaaacc gacgtagcca gcctgcgcaa acagctggcc ggcacagcgc
     1081 ctggtaaatc agagcaactg caacaacaac gtgttgaagc agaagatctt tctgcctttg
     1141 gtcggggcta ccgtattcgt gaagatcgct ttagctactc tttcaaccca acactttcac
     1201 agtcgctggg cggcccggaa gatttctata tgttccagct ggggctgatg tccagcgccc
     1261 gctactggtt taccgaccac ctgctgcttg atgacggtat tttcaccaat atttacaaca
     1321 actacgacaa gtttaagtct tcactgttac ccgcggactc taccctgccc cgcgtgcgca
     1381 cgcatatccg tgattacgtt cgcaatgacg tttatctcaa caacttgcaa gcgaattact
     1441 ttgccgactt aggcaatggt ttctatggcc aggtgtatgg cggttatctg gaaacgatgt
     1501 atgccggtgt cggttccgag cttctttatc gcccgctaga tgccagctgg gcgctgggtg
     1561 tggacgttaa ctacgtgaag cagcgtgact gggacaacat gatgcgcttc accgattatt
     1621 ccacgcccac aggtttcgtg acggcttact ggaacccgcc gacgctcaat ggcgtactga
     1681 tgaaacttag cgttgggcaa tatctggcaa aagataaagg cgcaacgatc gacgtcgcca
     1741 aacgctttga cagcggcgtg gcggtagggg tatgggcggc aatcagtaac gtatctaaag
     1801 atgactacgg cgaaggcggc tttagtaaag gtttttatat ctcgattccg ttcgacttga
     1861 tgaccattgg acctaaccgc aaccgcgcgg tggtttcgtg gacaccattg acgcgtgatg
     1921 gtggacaaat gctgtcacgc aaataccagc tcgatccaat gacggcagag cgagaagtac
     1981 cggttggaca ataacctgac gtaattcata acattacatt gactgataaa tataaaccaa
     2041 tgaaaaagaa catttttaag ttttcggtac tgacattggc tgttctgtct ttgacagcat
     2101 gtacccttgt tcctggtcag aatttatcga ccagtaacaa ggatgtcatt gaactacctg
     2161 acaatcagta tgacctggac aaaatggtca atatctatcc cgtcacgcca ggattgattg
     2221 atcagctgcg cgccaaaccg attatgtcgc aggctaaccc ggagctggag cagcaaatcg
     2281 caaactatga atatcgtatc gggattggtg atgtgttgat ggttacggtc tgggatcacc
     2341 cggaactaac cacccccgca gggcaatacc gtagcgccag tgataccggc aactgggtta
     2401 acgccgatgg tgcgattttc tacccctata ttggtcgcct gaaagtggcc gggaaaacac
     2461 ttacccaggt acgcaacgag attacagcgc gtctcgattc agtcattgaa agcccgcaag
     2521 tcgatgtcag cgtcgcggcg ttccgatcgc aaaaagcgta cgtcaccggc gaagtcagca
     2581 aatcgggtca gcagccgatt accaatattc ctctgaccat catggatgcg attaacgccg
     2641 cgggcggtct gaccgcagat gcagactggc gcaacgtggt gctcacccag aatggcgtaa
     2701 agaccaaagt gaacctctat gcgctgatgc agcgtggcga cttgcgccag aacaaactgc
     2761 tgcacccggg cgatatttta tttattccgc gcaacgatga tctgaaagtc ttcgtgatgg
     2821 gtgaagtggg taaacagagc acgctgaaaa tggaccgcag cggtatgacc ctggcagaag
     2881 cgctgggtaa cgctgagggt atgaatcagg atgtcgctga tgcgacgggg atttttgtta
     2941 ttcgtgcaac gcaaaacaag cagaatggca aaatcgccaa tatttatcag ctgaatgcga
     3001 aggacgcatc ggcaatgata ctcggaacgg aattccagct tgaaccttac gacattgttt
     3061 atgtcactac cgcgccgctg gctcgttgga accgtgtgat ttctctgctg gtaccgacga
     3121 tttccggggt gcatgacctg acagagactt cacggtggat tcaaacatgg cccaactaaa
     3181 atttaactca atcctagtgg tttgtaccgg caacatttgc cgttcgccca ttggcgagcg
     3241 cttgctgcgt aaacgtctgc cgggcgtaaa agtcaaatcg gcaggtgttc atggtctggt
     3301 aaaacaccct gcagatgcga cagcggcaga tgttgccgct aatcatggcg tatctcttga
     3361 aggacatgcc ggacgtaagc tcactgctga gatggcacga aattacgatc tgattctggc
     3421 aatggagtcg gaacatattg ctcaggttac cgcaattgct cccgaggtcc gcgggaagac
     3481 aatgctgttt gggcaatggc tggaacagaa agagatcccg gatccctatc gtaaaagtca
     3541 ggacgcattt gaacatgtct acggtatgtt ggagcgcgcc agtcaggaat gggcgaagcg
     3601 cctcagccgg taatttgagt ttataaaata cgatgacaac taaaaatatg aatacgccac
     3661 caggcagcac tcaggaaaat gagatcgatc tgcttcgtct ggtcggcgag ttatgggatc
     3721 accgtaagtt tattatcagc gtgaccgcgt tattcacgct gatcgctgtc gcttactcgc
     3781 tgttaagcac accaatttat caggcagata ctctggtcca ggttgagcaa aaacagggca
     3841 acgccattct cagcggcctg agcgatatga tccctaactc atcgcccgag tctgcaccgg
     3901 agatccaact gctgcaatcg cgcatgattc tcggtaaaac cattgctgaa ctgaatctgc
     3961 gcgacatagt tgagcagaag tattttccga ttgtgggtcg cggctgggcg agattaacca
     4021 aagaaaaact aggtgagctg gcgatcagct ggatgcatat tccacaactg aatggtcagg
     4081 atcagcaact gacactcacg gttggggaaa acggccacta tacactggaa ggtgaagagt
     4141 tcaccgtcaa tggtatggtc ggccagcgtc tggaaaaaga tggcgttgcg ctgactatcg
     4201 cggacattaa ggccaaacca ggaacacagt ttgtcctgag ccagcgtacc gaactggaag
     4261 cgattaacgc attgcaggaa acctttaccg ttagcgaacg cagtaaagaa agcgggatgc
     4321 tggaacttac catgactggt gatgatcccc agttgattac tcgtattctg aacagcatcg
     4381 ctaacaacta tttgcaacag aatatcgctc gccaggcggc gcaggattca caaagccttg
     4441 aattcttaca gcgccagtta cctgaagtgc gcagcgagct ggaccaggcg gaagaaaaac
     4501 tcaacgttta tcgccagcag cgcgattcgg ttgaccttaa cctggaagcc aaagccgttc
     4561 ttgagcagat tgtgaacgtt gataatcaac tcaatgagct gaccttccgc gaggcagaga
     4621 tctcccagct gtataagaaa gatcacccaa cttatcgtgc gctgctggaa aaacgccaga
     4681 cgctggagca agaacgcaaa cgcctgaata agcgggtatc ggcaatgcct tccacccaac
     4741 aggaagtgtt gcgtttaagt cgtgacgtag aagcgggccg tgcggtatat ctgcaattac
     4801 ttaaccgcca gcaggagttg agtatttcga aatccagtgc cattggtaac gtgcggatta
     4861 tcgacccggc agtcactcag ccgcagccag tgaaaccgaa aaaagcgttg aatgtggtgc
     4921 ttggttttat tcttggcctg tttatttcgg tgagtgccgt gctggcgcgt gcgatgttgc
     4981 gtcgtggtgt agaagccccg gaacaactgg aagagcacgg catcagcgtt tattccacta
     5041 tcccaatgtc cgagtggctg gataaacgca cccgtctgcg taagaaaaat ttattttcta
     5101 atcagcagcg ccatcgtact aaaaatatcc ccttcctggc ggtggataac ccggcggatt
     5161 ctgctgtgga agccgtacgt gcgctacgaa ccagtctgca tttcgctatg atggagactg
     5221 agaataacat tctgatgatc accggtgcga cgccagacag tggtaaaacg tttgtcagtt
     5281 caactctggc agcggtgatc gcccagtccg atcaaaaagt gttatttatt gatgccgact
     5341 tacgccgtgg ttattcgcat aacctgttta ccgtgagtaa tgaacatggc ttgtcggaat
     5401 atctggcagg taaagatgag ctcaacaaag tgatccagca ttttggcaaa ggaggctttg
     5461 atgtgattac tcgcggtcag gtgccaccta acccgtctga actgctgatg cgcgatcgga
     5521 tgcgtcaatt actggaatgg gcgaacgacc attacgatct ggtgattgtc gatacgccgc
     5581 cgatgctggc ggtgagcaat gctgcggtcg tgggacgctc tgtcggcacc agcctgctgg
     5641 ttgcgcgttt tggcttgaac accgccaaag aggtgagctt gtcaatgcag cgtctggaac
     5701 aggctggcgt taatattaaa ggcacaatcc tcaatggtgt gattaaacgc gccagcaccg
     5761 cttacagtta cggctataac tattacggtt atagttactc cgagaaagag taaaactgaa
     5821 agtgaccgga tgcggcgtga acgccttttc cggtctacgc attagacagt tcttcgtttg
     5881 tcatcagcct cagagcattc aggtaactga atgctctttt ttatgcatta caaactgcac
     5941 gccggtatgc gtgcttcatt cacgatttgc gtaaaacctg ccaacgaaca catgccctgc
     6001 gcatttcgct cttcacatcc tgccagggtc agtttcacct ctccgggcgg cgtatttaat
     6061 gacagcggcg ttttatcacg catctgctgt aaagtctgga agaccagcga aacctgaatc
     6121 cactggctgt tatcgcttag ccgacgccag cgttcaaaca ccagttcacc acctggcggc
     6181 gtgttatccg gctgacctgg aagcgtccag ttgagctcca gtgcgccgcc gagatttgcc
     6241 agattagtat cgtgtccggc aataaacagc actgaagtgg gtaatgtcac accatacgcc
     6301 tgtttttgcg gtggatgggg cgtcaacgct gccatgatca aatccaataa cggggtggcg
     6361 cgactgcggg caacctccgg cgtgcgttgt agtaaataaa attgcgcgtt atgcaaactt
     6421 agcaaggtgt tccactggtg tgaatcggtg atccttcccc accccggctc cggcattcct
     6481 tgtgcctgtt gcaggagaaa tatctccgtc agcattgatg cgaggcttac cgcaccggtt
     6541 aatgagacat tgtcggcgct caccttgagt tccgatggta atgcctgcgt taatgaacag
     6601 ctttcgtcct gtttctcacg gttaaggcac aagtttgatt gcgggaaatt aagcacccgt
     6661 tccagttcgc gaaacgccgt ttgccgatgc ccggtaaagt cagcaattga ccctcctgcc
     6721 ctgcagagga tcgcgtcagt cacgttcgcg ttatccagtt ggcaaacacc agtttttaga
     6781 ggattaaata acggatcggg actggacgta tctgcctggg tatgtacggt tattgcacag
     6841 tcaggtgcca gcccggcggc gaaggcttcg cctgttttac gggtacgctc gtcgacatca
     6901 gcaataatcg cgacctgagc agactgcggg cagccctttt tcgccagcaa tccgtcggct
     6961 accagacgct ggcgttggta atgtccgaga taggcgatta gctcaccacc gcgcggtgtc
     7021 agccaaccca gttttaccgg ccaggttggc catgcgtctg gggtgacatc ctgcatcagt
     7081 tgcgtggcct tggttggagc acgcacacca tgacgactga caatcaccat actttccagc
     7141 ttcagctccg gctcactctg agcgaatgca gattgcgggg ttaacggaat cagaagagat
     7201 aaaaatggga ttaagatcgc tttcatcgat atgtttccgc ttttcatatc taacattatt
     7261 gattgcctga tgcgatgcta atgcgccgca tcaggcctac gttcgttaac ttttcagacg
     7321 cgggtccagc cataccaaca caagggtgga gagcgaacac atcaacaaaa tgccgacgaa
     7381 ccaaagtaaa taccacatgt tcctgctcct tagtacaact cgttttcgtt acggcggaga
     7441 gtttctgttg tcatgcgtcc ccacattttg tagtagctcc agagagtgta gagcaacaca
     7501 atgggcaaaa atatcagcac gattaccaac ataatgctca gcgtcagctg actggaagtg
     7561 ctgtcccaca acgtcaggct ggagattgga ctcacacttg acggcatgac aaaggggaac
     7621 agcgtgatgc ctgccgtgaa aatcacgcca aattgcatca atgatgccat caaaaatccc
     7681 caacccgggc gaccacgata aatcgccatc accgtcagca atgggcagaa gaaccccagc
     7741 agcgggaaga tccacaaaac gggcgattcg acgaaattat tcatccaggc accaggtagc
     7801 actgccacca gtttcattaa cggattggaa ggaccgttag catcctgggc gagcagtaca
     7861 aagccatcaa taccgaccca cagccagtaa cctgccagca aaaagcacag catcaccaac
     7921 agtgcagcgc gtttagtcgc cagctgtgaa cgcagatgaa tcacaccaac agttttcagt
     7981 tgtaaccaga cgccaccttg caaaatcacc atcccaaggc tgagcaaacc acacaataaa
     8041 gggaatggcg tcagcagttg ccagaagctg ccgagatact ccacgcgtaa ttgcggtgtg
     8101 aaggcaaacg gcacgccgag caacaagttg ccgaaggcga taccgaagac taccggcggc
     8161 accagactgc cgatgaccag accggcatcc cacattttac gccagcgtgc atcggcaatt
     8221 tttccgcgat aatcaaaggc cagcggacgg aagaacagcg agcacagcac caggatcatc
     8281 gccacataaa agccggaaaa cgccgctgca tacactctgg gccaggcggc aaataatgcc
     8341 ccacctgcga ggatcaacca gacctgattg ccttcccagt gcgcaccaac gctattaatc
     8401 actatccggc gttcatcatc attacgcgcc accagcggca gcaaacagcc gatccccatg
     8461 tcaaatccgt cggagatcat aaagaccacc aggatcacgc caatcagcag ccaccagatg
     8521 aagcgcaatg tttcataatc aaacatgatt ttctccttta cccctgttgc tgcgtcggtt
     8581 gttcactctg catcgcgctc ggccccagac gggcatattt ctgcatcagg tagacttcgg
     8641 cgattaagaa cagggtgtaa agccctacga tcatgatcag tgagaaagcc agttgtcctg
     8701 tggttaaagc ggagtgcgcg gagtatgtcg gtaagatgtc ctgtatcgcc cacggctgac
     8761 gaccaaactc ggtcataaac cacccggctt caatcgcaat ccacggcaac ggcaaactcc
     8821 agagcgccat tttcagcacc cagcgatgct ggtcgatttt gccacgcagc gtctggacaa
     8881 gcgcaatcag catcaccagt agcagcaggg aaccacagcc caccatgatg cggaaactcc
     8941 agaataccgg cgcaacctga ggtatcgcgc cacgcatcgc cgcctggtac tgtgcggctg
     9001 tgacatgatt catatccggc gcatagcggg agagcaacat gccgtaacca aggtcgcctt
     9061 ccagctcccg gaatgcctgc aacacatgcg gctcacgatt gccttgagat atttcctgca
     9121 ttaacagcca ggccatacgt ccgcgttgca agcgtgggta ggtttcagcc atcaaattct
     9181 tcagacccgg cacgggttta tctaatgagt gagtggcgag gatccctagc agcgcgggaa
     9241 ttttgatggc aaaggcgtta cgctcttgat cctgttccgg ccaggcaacc acatggaacg
     9301 gtgcaggagc aggttccgtt tgccactccc cttccatcgc cgccagtttt accggttgta
     9361 cttgcgcgac ttcatacgca gaactgtctc cgagttgcag ggtaccgata atcgccagag
     9421 taccgaagac ggaaccgatg gcaaacgagc gtaatgcgac atcgcgctcc cgtccgcgca
     9481 gtaaatacca ggcgctgatc gccataataa acatggcccc ggtcacgtag cccgccatga
     9541 cggtgtgcac aaatttcacc tggctgaccg ggttaaagac cagctcgctg aagctggtca
     9601 tctccatacg cagggtgtcg atatcaaaat gcgcaccggt cgggtattgc atccaaccgt
     9661 tggcattcaa tatccacaat gcagagagat ttgaaccgaa cgccaccagc cacgtcacca
     9721 gcaggtgctg gtatttattc agccgttgcc agccgaagaa gaacagcccg acaaaggtgg
     9781 attcgaggaa gaaggccatt aatgcttcca tcgccagtgg tgcgccaaaa atatcgccca
     9841 catagttgga atagaatgac cagttagtac caaactgaaa ctccatggtc aggccggtag
     9901 ccacgccaag agcaaaattg ataccgaaga gcttacccca gaagcgcgtc atatcgcggt
     9961 agattgtttt gccggtgacc acgtaaatgg tttccatgat agccagcaaa aaaatcagcc
    10021 ccagggtaag gggtacaaat aaaaagtgat acagcgcggt cagagcaaac tgccagcgcg
    10081 ataaatcaat gacatcccac atgcgcactc ctgtaggcga agagagaaaa ataacccgat
    10141 aatgttagat ctccctcttt atcctgaaac tctacgacca gaggtattcg ccgattgcga
    10201 tattcataag actgaaagcg atacttaccg tctttttaca tcggtgcagc ttccgccagc
    10261 cactgacata cctcgctaag ccgctgcgca gagtcgacca aatcttctgg cgctgccagc
    10321 accacttccg gtattgggca gatttcataa ctttccagta acgggccttt taaggtgtcc
    10381 gtacagcgta aatgccagac atggcgtaac cccgtggcgt tgatatagct ctcgccatag
    10441 ccaatggtac gaatctgaat atttcccggc ccacagagac gcgagagaaa aagccgatca
    10501 gcctcgctga tgggtaattg cgtcagatta atgctgtgcg gctgcgcgtc ggggttacgt
    10561 acatgtgcca gtaactcatg cgccaacggt aggccattca tcaggccatc aatgggcggc
    10621 ggtaacagcg aatctgtcgg caagagattt tgcgttgccg cctgccacaa cgc
//
LOCUS       DADLDT020000127        10662 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000127 DADLDT020000000
VERSION     DADLDT020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.128"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="JQH22_003388"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="JQH22_003388"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFB2207601.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="JQH22_003389"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="JQH22_003389"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFB2207602.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4036..4278
                     /locus_tag="JQH22_003390"
     CDS             4036..4278
                     /locus_tag="JQH22_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207603.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4392..5030
                     /locus_tag="JQH22_003391"
     CDS             4392..5030
                     /locus_tag="JQH22_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFB2207604.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5027..5764
                     /locus_tag="JQH22_003392"
     CDS             5027..5764
                     /locus_tag="JQH22_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HFB2207605.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5764..7860
                     /locus_tag="JQH22_003393"
     CDS             5764..7860
                     /locus_tag="JQH22_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFB2207606.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7907..8185)
                     /locus_tag="JQH22_003394"
     CDS             complement(7907..8185)
                     /locus_tag="JQH22_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207607.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8186..8329
                     /locus_tag="JQH22_003395"
     CDS             8186..8329
                     /locus_tag="JQH22_003395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207608.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8455..8865
                     /gene="psiE"
                     /locus_tag="JQH22_003396"
     CDS             8455..8865
                     /gene="psiE"
                     /locus_tag="JQH22_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFB2207609.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="JQH22_003397"
     CDS             complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="JQH22_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFB2207610.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2707 a         2722 c         2763 g         2470 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca agttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 tttttatttt aagcttatga tttttattgt tatttaaata taagttgaaa cttatatttg
     3901 atattcattc caattatcct aaaactccat cgctaattcc ccgcgccgta attcgcatgc
     3961 tttagttgtg tatactcgat cccgcccgaa atgtttttgg gtaaatctcc attcattcaa
     4021 tgaagggaaa ttgttatgaa aaaagttctg tatggcattt ttgccatatc tgcgcttgcg
     4081 gcgacttctg cgtgggctgc acctgtacag gtgggcgaag cggcagagtc ggcagcaacg
     4141 tcggtttcgg cggggagttc ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt
     4201 gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata ccgggaccat cacaaccacg
     4261 accaccagta cccagtaata aagtatgtat ccccaaaata atttgagtca ttgcatctgt
     4321 ggcttgaagt atgaagggat ttaccataac cacactccgg tgtggttatt ctgcccctct
     4381 ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta caggcctgtt
     4441 cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggc acgccaggcg
     4501 tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac atgcagctta
     4561 atggcgggcc gcaattgttt gtggtgctgg ccttcgctga agacggacaa caaaaatggg
     4621 tcactcagga tcaagccact cttgtcacac aacatggtcg tctggtgaag actttgcttg
     4681 gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt aaacccgcac
     4741 aaattgttga tggtgcaaca tggacgcgca cgatgggctg gaccgagtac cagcaggtac
     4801 gctacgccac cgcacgctca gtcttcaaat gggatggcac cgataccgtc aaggtcggca
     4861 gcgatgaaac cctggttcgc gtgctggacg aagaagtctc caccgaccag gcgcgctggc
     4921 ataaccgcta ctggatcgac agcgaagggc aaattcgcca gtcggaacag tatctcggcg
     4981 cggattattt cccggtgaaa accactctca tcaaggcggc aaaacaatga ttaaacaaac
     5041 tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa
     5101 ggtgttcagc aatggcagca gtgaggccaa aacgctaacg ggcgcagagc atttaatcga
     5161 tctggtaggg caacctcgac tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga
     5221 gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca
     5281 gggcgcagat tccagcaccg acgatgccgc cgccattaac gccttacgcc agcaaatcca
     5341 ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc
     5401 cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggac caccgccgtc
     5461 cacggtcacg ttgttcgggc ttatcagcca tcctggcaat cagccattca ctccaggtcg
     5521 cgacgtggcg agctatctct ctgaccagag cctgctcagc ggtgcggatc gcagctacgc
     5581 gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg gtggcttact ggaacaagcg
     5641 tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcagatt ccgtctggag
     5701 tgagacgcct gatgccctta acgccgacat tcttcagact ctgacgcagc ggatacccca
     5761 ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac agcttgctac
     5821 ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg atttcggtgg cgtaggatta
     5881 ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat
     5941 aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct ggaaacaacg
     6001 ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt ctctggcgat
     6061 caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga gagttactgg
     6121 ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt tgatgcggaa
     6181 tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct gggctgggga
     6241 tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt cagccagtga taaatattgc
     6301 tatcgcgata acagctacaa acaggcggga tctatcgacg gtagccagat gttccacggt
     6361 cctgcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct gcgtctgaaa
     6421 ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga gcaaaaaagt
     6481 aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa ccttagctat
     6541 gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc
     6601 ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc agccgcagga tgccattttg
     6661 cagcattcgg tagtggcgaa tcagttaacg ttgctgaaat acaacgccgg acttgccgat
     6721 ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt
     6781 gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct gccggatggg
     6841 atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaagtgac gacggaaacc
     6901 gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt tgggccacga aacgacgctg
     6961 gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg gtatatcgac
     7021 aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa
     7081 aacttttaca tgtatcagct gggcgtgatg ggaacggcag atttgtggct gacggaccat
     7141 ctactgacca ccggcagcct gtttgcaaat cttgccaaca actacgacaa gtttaactac
     7201 accaatccac cgcaggactc gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg
     7261 cagaacgatg tctatgtgaa taacctgcaa gccaactact tccagcatct gggcaacggc
     7321 ttctacggtc aggtctatgg aggttatctc gaaaccatgt ttggcggtgc gggggccgaa
     7381 gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa
     7441 cagcgcgact ggcgtagtgc aaaagatatg atgaaattca ccgactacag cgtgaaaacc
     7501 ggacatctga ccgcctactg gacgccatct ttcgcccagg acgtgttggt gaaagccagc
     7561 gtcgggcagt atctggcagg ggataaaggc ggcacgctgg agatcgctaa acgctttgac
     7621 agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg tttcgaaaga ggagtacggc
     7681 gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc
     7741 ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg tggtcagcaa
     7801 cttgggcgta agttccagtt gtatgacatg accagcgacc gtagcgtaaa tttccgctaa
     7861 gtcatgggaa aggtgccagt tttcgcactc actggcaccc tcattcttaa tgacctgttg
     7921 agccatcagg aacgcagttc acccccggtt tacgatctgg tgtgtaaagg cgaaaacatt
     7981 ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac tgggccatca
     8041 ttgggctgga gacattgctt tgcggtaggt cggctttatc agcagtaccg agcggaccag
     8101 cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc ttcattttta
     8161 ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat
     8221 tgctggtggc tgggaatcac ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt
     8281 tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc tacgggtagg tatatccggc
     8341 tttggtggag gcgcgctcca aatccaggtt gaacaaaaca tacacaaaaa atatagatct
     8401 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     8461 tcactctctc gtccgcgcgt ggagtttatc tccaccattt tgcagaccgt actcaatctt
     8521 ggcctgctgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     8581 gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg agctggtaga aggactggtg
     8641 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     8701 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     8761 gtcgatcata aatcgccact ggatgtgttg atctactcgg ctgcgatcct gttactggtg
     8821 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg
     8881 aaatggcgcg gcgtgctgga caggaagatt acagcgtagc agtttgttgt gttttcttcg
     8941 tttccggttc ccagagcgct tccagctcct caagggtttt acctttggtt tccgggacaa
     9001 atttccacat aaacagtgct gccagaacgc ccatacaacc gtaaatccag taggagaaac
     9061 cgttgtggaa atgggccacc agccaggagt ttttgtccat catcgggaag gtccaggaga
     9121 cgaagtagtt cgccagccac tgggccgcca ccgcgattgc cagcgcttta ccacgaatag
     9181 cattcgggaa gatttccgac agcagtaccc agcataccgg accccaggac atggcaaagg
     9241 cggcaacata gaacagcatc gacagtagcg ccacaatacc cgatgcctga gtgtaaaacg
     9301 cggtaccgag gctaaacata ccgattgcca ttccgagtgc gccgataatt tgcagtggct
     9361 tacgaccaaa tttatccaca gtcataattg ccagtacggt aaaggtgagg ttgataactc
     9421 cgacaataat ggtctgcaac agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt
     9481 ccggcgcgta gtacagcacc acattgatgc cgacaaattg ctggaagatg gagagcataa
     9541 cgccgattac aatcacgccc acgccaaaca tcagcagacg accaccggtt ttgcggccat
     9601 gatccaggga gtgtttaatt tcctgtactg cctgagttgc aagcgtgttg cccataattt
     9661 tgcgcaggat actttccgtc tgttcttgct tgccgcgtga catcagccag cgaggacttt
     9721 ctggcacggt atacagcagc attaagaaca gcagtgcagg gatacattcc gaggcaaaca
     9781 tataacgcct gccgtcagta ttcagccagc tggcatcacc ggaacgggca ataaaatagt
     9841 ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt
     9901 ttccgcgaat atgagctgga gccagttccg caatatacat tggtgaaagc attgaggcta
     9961 aaccaacgcc aataccgcca ataatacgat aaataacaaa ttccgggaca taacctgcca
    10021 gataaatagg tactgtgttg tccgggttta tagaggtaaa accaagttct ggccaggcag
    10081 aacctacacc agaaataaaa aacaggacag cagaaatctt aagtgaatca cgacggccga
    10141 agcggttact gcaataacca ccgagggcac cgccgatgat gcaaccaatc agagcgctgg
    10201 ctacgcaaaa tcctaacagg gagttggcag cggattcact taagttttgt ggagcaacaa
    10261 agacggtatt gagtgactca acagtaccgg aaataacggc ggtgtcgtag ccaaataata
    10321 aaccacctaa tgtagcgact aaggtaatcg aaaatatata actggaatta tactgggtat
    10381 tcattcagac ctgccttaga ccattctgat cttttatcag tgcattgatg ttggaattgt
    10441 caggcagtta aggcaattat cattttttac aatgcgttta cgtaatgtct tttctgtgat
    10501 cttaattgtg ataattatca aaaattgatg aaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aa
//
LOCUS       DADLDT020000128        10584 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000128 DADLDT020000000
VERSION     DADLDT020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.129"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            70..501
                     /locus_tag="JQH22_003398"
     CDS             70..501
                     /locus_tag="JQH22_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207611.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(533..1060)
                     /locus_tag="JQH22_003399"
     CDS             complement(533..1060)
                     /locus_tag="JQH22_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFB2207612.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(1053..1799)
                     /locus_tag="JQH22_003400"
     CDS             complement(1053..1799)
                     /locus_tag="JQH22_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFB2207613.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1814..3721)
                     /locus_tag="JQH22_003401"
     CDS             complement(1814..3721)
                     /locus_tag="JQH22_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFB2207614.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4430..4822)
                     /locus_tag="JQH22_003402"
     CDS             complement(4430..4822)
                     /locus_tag="JQH22_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HFB2207615.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4819..7020)
                     /locus_tag="JQH22_003403"
     CDS             complement(4819..7020)
                     /locus_tag="JQH22_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFB2207616.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(7017..7325)
                     /locus_tag="JQH22_003404"
     CDS             complement(7017..7325)
                     /locus_tag="JQH22_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207617.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7301..7636)
                     /locus_tag="JQH22_003405"
     CDS             complement(7301..7636)
                     /locus_tag="JQH22_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207618.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7663..7980)
                     /locus_tag="JQH22_003406"
     CDS             complement(7663..7980)
                     /locus_tag="JQH22_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207619.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(8071..8472)
                     /locus_tag="JQH22_003407"
     CDS             complement(8071..8472)
                     /locus_tag="JQH22_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207620.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            complement(8459..9508)
                     /gene="virB11"
                     /locus_tag="JQH22_003408"
     CDS             complement(8459..9508)
                     /gene="virB11"
                     /locus_tag="JQH22_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFB2207621.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            complement(9512..>10584)
                     /locus_tag="JQH22_003409"
     CDS             complement(9512..>10584)
                     /locus_tag="JQH22_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFB2207622.1"
                     /translation="VVQHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGK
                     SASHEPESHATSPARERNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFD
                     KVSALADSAPTTASSAPKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLG
                     LDPDLYIPADRHIPCAMMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYR
                     TGTLKNGQGRLFLAITELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGN
                     TLLLGTVQDFAAAASGSSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQG
                     DVIGLVTGADIDFSDVYRLRMR"
BASE COUNT         2131 a         3003 c         2996 g         2454 t
ORIGIN      
        1 gaacgactcc gggcaggccg aaccgtcagg cgatacgaag acgaaattca cactgacgga
       61 gaaacgacca tgacgatgaa cctgcacacc ccgacaaccg cccctaaagc tgcaccggcg
      121 accagcgtat ccccgctgga gcagtcaggc tccccaaaaa cgaaatttcc tgaaatcaaa
      181 accgtcaaaa cggcggtggt taaactgatc agcgacatcg agggcaacct cgatcagcgt
      241 ctggtatatc gcgaggccgt agagggattg tgccaggagg cggccaaccg gataacccag
      301 tgcatgcatt cacgcagtac agggcagacg ctacgtgatc tccgcgagct gctgtcggtg
      361 acgctggaca ccattatcgt ggtggagttt gaaggggagc agacccgtct caactatggc
      421 ggacgttcag cagaagacgt gatcgccatg ctgaaagcgc tgcatcttga aggacgcaaa
      481 gcctggctgg aagtggagta aaaaaaaagc cccgtccgca gacggggctg attcagtaca
      541 gggcattcag cgggatgccc tcatcccaca ggcggttaaa ttctcgctgg tacgcctccg
      601 ccagctgcgg cgcatcctcg atcagcagca cgttttccgc gttgcgcgaa acggcgcttg
      661 ccgtgtagtt aaacgacccg gtctggacgt ttttgccgtc gatcaccata aacttgttgt
      721 gcatgatggc gtaacggccg ttaaggtgca ccggcacgcc ctggtttatc agatacgtca
      781 cggcggtgta gcggtcgctg ttcgcctttt cgtcggccac cacgcgcacg gccacgccgc
      841 gccggttagc accggcgagc gccgtcgcga ccggcttact ggtgaagctg taggccgcga
      901 cgtcaatgct ctgcctggcg ttgtttatgg cgccgagcac gatttcaaga gcagggcgcc
      961 cgtcagacgg cgaaaaaccc gccgtcagga cgggcgcggc ggtggcgaac gcgggcacgc
     1021 agagggcggc ggccagcacc agaagggtgg ttttacgcat agttgtccag ctccaggttt
     1081 ttcagttcat cgagggtgta aaagcggaaa acgtcgcggt gttttgcctc cagttgcaca
     1141 tgctggtttt tcacgatcac gctggcgatg ctgtcgaaaa taatcttcag ggcgcgcttt
     1201 ttcgcctcgt ccggggcgac gtaaaacgcg taccgccatt ttttatcgct gatagccagc
     1261 aggtggctcg ccatgatgga ctgatagcgc gcctttgttt tgatacgtcg ttcggtttca
     1321 acggcgatca gcctgccgtc cggcaggctg ataagcccgt cgggacggtg tttgacgctg
     1381 tactgactga ggaacgttga acggtcgccg ttaatccacc ctgtcgcgcc gttcttctcc
     1441 agcgtaaggc gcactagctg gttatcaagg tgatggtcga gtgaccagcc ggtcaggcgg
     1501 tagggttcaa agcgggcggg cattatgttg tcgtccgccg tgaggataac ggccagcccg
     1561 tcggtggtaa tgccccacag ggatattctc gacgcgcggg attcatgcac gtgcttctga
     1621 atcagcccca ttttctccac cttctccaac aaccggtaga gagggcggtc agatttaaaa
     1681 tcaaaaagca gctttatgat ttgaaaatcg ctgtaggttt cctccttgag aaaattcagc
     1741 agtacgcgaa ttttttcagt gctgcgtgca taacgttcct gatacgacga aataagcata
     1801 gaaactcctt attttataaa tccagcaacg aatgtatttc gtcatttgga gcgggctgtg
     1861 atgccatcag ccactcctcg tcaggttgca ccggcggagt gggtattggc gttgctgagg
     1921 gttttttggt ggttgttgcg ctgaccggaa taatgtcagg tgccggaact gccggagccg
     1981 ttttatcaaa atccagatcc agcaccggtt taacttctgc caccgtagcg actgcgaccg
     2041 gcggcagggt aaacagctcc agtggggctt ttttaacctt gagcggtgag attaacgacg
     2101 ggaacgcgag cgcgttctgg ctgaagatat agctgacgaa cttcggcagg ttctggagca
     2161 tattgctgtc gatgtaatag ctttccgcct gccgaagcat gcgctcgtta tcgacttttt
     2221 ctgtcagcac cgccgtggtg gtgactttcc ggctttcatc atcgactaaa atcgtgcccg
     2281 acatgcgcgc aatccattcg gcggtgtctg gatcctgaag tttatatacc agcttaaact
     2341 tgcagttttc cacaaccgcc ccaaccacgg cgtcgccgtt taaatccgcc ggacagtcgc
     2401 gcaggtcggc aattgactgg tgcgccatta atatatggac gcctttatcg cgtgccgcgc
     2461 ctaacccttc gagcgccggt ttggacaggt gatatttcac ctcgtcctta aagatggcga
     2521 tggggcggat attccccgcg acgcggtcgc gcaattccgc gagctggaac aggcgaacta
     2581 aaatcatgcg ctgcgcggta atgattttag agttacgcat cgaaccgatg atatagcagc
     2641 agccgccctt gtcgaaaatc tccttcagcg caaaaccgcc tggcgcgtta atgctgttga
     2701 gcattgccag ctcctcggtt ttaccgtgaa aggctttaat cgtctcgcca atgccctgaa
     2761 cataatcgcc gttataaata tcccgcacgg ttgcgcccgg attatccttc acgaactgcg
     2821 ccgccgttct cgccgccttg cggtcgtcga tacggtaaaa atccgccgcc tcgccttttt
     2881 ccgccagact gaaaccggcc acgaataatt cctccagatg gtcggcggtg ataccgtcga
     2941 taaggttcat ctggtattcc tgtttattca ggtcgataag cacaaagggt tttccggcgt
     3001 ccgcgcacgc ttttttatac aggtgcggtg cccactcgtc gtttttgggg tcgagaacaa
     3061 aaacgccctc gtcggcgagg atgctctgat aaagcagaat accggttacg acgcctttac
     3121 cggcaccagt ggtgccgata atgtcggcgt gctgggtctg ccattctttt aacggaatat
     3181 attgcggctc accctcgcgg ctcagcccga taaaaatccc cttattcaga tcgatgtaat
     3241 ccatcggatc gtattctgtg gtgacgggta aaatttcttt tacccggcga acgtcggttc
     3301 tgcggtcgcg ctccatgctg gtttttttca ccatgccgca tttgacctta tcaaattcag
     3361 gcgagatcaa acgccgtaaa accacgtgca gaataaaacc ggcgacggta aacagtagcg
     3421 ccattgtcca ggtaagcagc ggcggactgg cgatatcttc ccggccataa atgcgctgta
     3481 aaaaccagac ggccactggc gctatcgagc cgaaaacaaa acagatcatg gaaagcagaa
     3541 ttaagatcct ttgcagaaac gtcggctttt cctgaccgcc gtgtttcgca gaaaatataa
     3601 aaggcagcat gacgccggac agcagcgcta acgccagttg atgcgcctgc aaaaaggcga
     3661 taaaggccag gacggcattt gctatcggcg acagcaaatg attcagtttg tcgaatatca
     3721 cgatattctc cttcacttaa attcggtcag caccatcggt cagtcagaac ggtcacaggt
     3781 tgtgatcgtt ctgactgacc gattccatgc tgcaaaagaa gtggccgcta agcggccatg
     3841 atcgcaggag gtacactccc gcgatctaac ggcgtgcggc attacgcgcg ggctggacgc
     3901 ccttactgca tgtcgcatgg gggtaatgtc ctggctgaac gcccggccaa aacccccaag
     3961 gtcaaaagag gccacaccgt cgcgcgctac gcgcgaccac cccctaaaag acgcctgttt
     4021 tagccctttt cttcgccttg ttcgttcctc acaagacgaa ttaaagggaa actaggcttt
     4081 ttagggggtg gtcgcgccac gctaccggct tttggccgga gacttaggca cgacggtgtg
     4141 gggggcaagc cccccaaata aaccgacgcg tgggcgcgtc tttttgctcg ctggggcggc
     4201 aaaatttatt cggccccccc cggctttttt cggctcactt ggggtgagcg ctcaaaaagt
     4261 ggaatcggcg acgcaaatta gcaatttctt cgaaattgaa tttactcgcc tcctgccact
     4321 tcgcagactc aggcggcctg tcgattagca attttttcaa aattgaatcg ccatgctcgc
     4381 cagtcttcga agcccgaaaa gccaggtcaa aaccgaccct gtgccgttgt tagtcggtgg
     4441 taataaggaa ggattcaaga ggttttccgg cgtcaagttc ttgctgaatt acaaccggca
     4501 cgcgtccgac gcctgaccat tttttatgca cgccgttttc aaaatactga tatttagctt
     4561 tgcgttttgc cgactttttg gcgggagttg tactaaccag ctctgtcggt gaaaagccct
     4621 ccgattcaat cagcgccagc aattcattgc gcttttcttc ccgctgtcgg cgtagctttt
     4681 cctgctcgat atgctctgcg cgtttttcat caagcgctgc tgaaatacgt gcactcagca
     4741 tctccagaaa ggcaaaatca ctttcacgta aaaacttttt cagcgcgcgc tctgaggtta
     4801 attcgtcctg gatacggttc ataatttcag ctccttcggc atagatacgt tcaatccttt
     4861 cagcggatcg cgtggtggat attcaaaacc gagttccccg gtttgcgcgt ttttcagcgt
     4921 gacgatcatc gaataggtgt cgtcgttttt cttcgattta aaacctttaa taacgtcggt
     4981 tttgcccttg ccgattaact tctcggcctg tttttccgtc agctttttgc cgcgcacttc
     5041 cagccatatt ttgaactgac agcccgtaca ggcgcaggtt ttcggcgtca taaccagctc
     5101 agaaccgcac gacggacatt taaccgcgag gcggttaagc gccgttttaa cgggatgttt
     5161 ttccgtcgcg ctgatggcaa tatcggcgct ctcaaccagg cgggaaactt ccgcgtaaag
     5221 accatcaata aagcgctcga tggtcatttc gccggtttca accgccgcct gtttttcagc
     5281 ccatacggcg gtcatatccg gctgcgtcac cgagtcgggc agggcatcaa ttaacgccag
     5341 cccggcggca gttgggataa atttgccctt ttccacggtg atgtaatcgc gggttttcag
     5401 cgtctcaata atagccgcgc gggtcgccgg tgtgccgatg ccgccgtgtt catctttttt
     5461 gtctttatcc ttctccttga gcaatttttt aatgcgcgga tcggcgacaa aatcggccac
     5521 gcgaacgagg gcagcgagca gggtcgcctc ggtaaacagc ggcagcggtt tagttttttt
     5581 ctccgaaatc accacctcgc ggcaggttaa tgtcgccccg gtttgcaggc ggctgatggc
     5641 ctcaaacgag gtgctgtttt cgtcctcctc gtcgtcttct tccgcgtcgt tcaggaacgt
     5701 cgtaaaaccc gcatcggtga ttttacgcgc cgagacttta aacatctcgt caccgcattt
     5761 aatctccgcg atcgactcgt catagcgctt tttcgccacg aactgcgcga gataaaactg
     5821 cgcgatggtg agataaaccg ccttttcctt gtcggtaagg cggcttaaat ccggcatgtt
     5881 cgtcgtcggg ataatggcgg tgtgggcggt gactttttta ctgtcaaacg ccgcactttt
     5941 ttgcgccgtg tcggtggtaa ggccgttaaa aacgtcgagc gtttttaagg ccgccaccac
     6001 ctgtggcgcg tcgttgaatt gttcatcgga cagataagaa cagtcagagc ggttgtaggt
     6061 aatgtattta ctgtccttga gcttctgcgt gatatcgagc gtctgctgcg cggtcagttt
     6121 gtgcctgcgg ttcatataca cctggaggcg cagcaggtta aacggcagcg gtgcggccgt
     6181 ggtttttccc cggtcggtgg cggccgcgag cacgctggcc ggttttccgg ccagagccct
     6241 cgcgagtcct tccccccagg ccttatccgt cagccgcttt ttatcgtcca ccggcgcgta
     6301 ttcggacgtc tgccagcggg cgcagacaac gtccgtaccg ctgataaact gccctgcgag
     6361 ctggtagtaa aacgcctcgc tgtgcgcctg attgtcgcgc caccggcgca caatcattcc
     6421 cagcaccggc gtctggacgc ggccaaccga aatcgtgccc ttatagccct tatcgcgggc
     6481 tttgagggtg taacagcggc tcatactgaa gccaaaaatc gcgtctcctg cgctgcgcat
     6541 cagggctttc aggtacatcc cacgaaaatc gctgttatcg cgcagggagt tgatggcttt
     6601 tttcaccgct ggcgcggtgt tgtcgttaat aagcgcccgt ttcaccggcg cggtgtttcc
     6661 ggcgaactcc agcacctcgt caaccaggag ctgcccctcg tcgtccgggt cgccgcagtg
     6721 gacaaccatt ttgatatccg ggcgccgaat cagcttcacc agggtattag cctgctttgc
     6781 cacgctcgct tttggctgaa gctgtaccgg gtacagcttc agcggcaggg tgtcgagcga
     6841 ccaggtggcg taggccgggt tgatcgtctc cgggggcacc atttccagca cgtggccgac
     6901 gcagtagctc acgatatcgg tcgccgattc ggcatagccg tcgtgccggg tgaagttgcc
     6961 gcccagcgct ttcgcgatat cgctcgcgac tgcgggtttt tctgcaatcc acagtctcat
     7021 tcttgatctc tcctgatttc atccatcgtt tcgaagtaca tcattcgact ttcctgaaag
     7081 tcgttatcag acagactaaa gccatgatcc gccgctactg tcatgaacgt ctcagcatca
     7141 cggacgctga gactggtcag aacatcgtgc aattcgcaca gggcgcgata ttggtagatt
     7201 tcgtaatgca ggccgcagcc gcgatgcgct tctttctcga tgcgagaaat caggctcgcg
     7261 gcactaacgg aatcaccacg cacatgcggc cacggcgggg ctataaatcc ggtttttgtt
     7321 gccattggag tgcatcctta tcgttgagtt tttcccattc cgccgcctcg tcgtcgctca
     7381 gcggaacggt gtaaaagaag accggcatac tgagcacgga gcggaagaac gactgacgtt
     7441 ctgctggtaa tgcctggaag gtcggcgagt gctgcgcggc gagctggacg agttcggacg
     7501 tgttggcctg aaaatgatcc tgtttctggc tgatttccga caaaacgagc agcaccgtct
     7561 gcaaatcacc tgcaggaagc tcctcatctt tcagcagccc ggaaaagtta ttaatagaaa
     7621 cggtttgatt aatcacgtgg tgtctccttt acggctgccg ttttaccaac cgttcatacg
     7681 gccaaatttc gagatgatct tgccgacaat ggcgctgtca gcggacgggc aggcgctcag
     7741 ttttttctgc cgcgctgcgg cggtgtgatc gggtaaaaac gacccctttt ttttcttttt
     7801 gatgctcatg aaatttttgt acatcgggtc acactcggcg ggactgctgc cgtcgagctt
     7861 tccggccagg cacagcacca tcgcgcacgg gtcattcggg tcggcattct ggccatcggc
     7921 ggcgaacgcc ggagaagtaa gggcgcacag cagcgccgcc gtgattacgg tgtgtttcat
     7981 gatgatttcc tctgaaggga agcagggcgc taacgcccgt tggttgttca acgtcggtga
     8041 cggtctgaag gactcagacc gtcaccgacg ttatcggtag aagacgattt ctgaggtgtc
     8101 gcaggtcagg cagccggagc gggggcgaaa ctccaccaca cccttgtagc cgccctcgcg
     8161 gagccaggtt ttggccgcaa agaagcgctg gccgtccggg gcatttacta ccgcgcgcga
     8221 ggcgtgcgca accgcgtacc actgtgcggc cgggtaaatc ccgccgggtt caaaacgcgt
     8281 aagcacctgc gaccagtgcc cttccgtcac cggcgaggcc acgataagac ggttgggttc
     8341 ccataccggc cgggtggtcg ttaccggctg cgccggttgt gaccagtcaa cggccgcagg
     8401 ctccggtggc gaagaacagc ccgccaggag gacgacgatc gccgtcaggc cggtcagttt
     8461 aggcagcatc attgaaactc tccttcatag cctgcatgtg caggggttta aaatagacgt
     8521 cgctcatgcg gcgcacgttg ccgtgaacgt ccacgctcac aatgacgtcg acgcagtgca
     8581 gtaccttgcg cagcagcacg tcaaccggca tgccccggca cgccggatgc tcgtagcagc
     8641 ggtcaatcag cccggagata cactcgcgcg ggctgcccgc gtgcagggag gacataaacc
     8701 cctcgtgccc ggactcaatc agcttgagtg cgtcccacgc ctcgcggcca cgcacctcgg
     8761 cgagcagggg gcggtcgggg ttcatacggt aaatcgcgcg caccaggcga tcagaggtga
     8821 ctattgcgcc ctctccggcg tccgccgggt agaacagatg aacgtagttt ttctggctgt
     8881 aaaacttcag ctccgggttg tcctcgatgg tgataaggcg cagcgtctcg ggaatgtagt
     8941 gcagcagcat cttcatgtag gtggttttcc cagagcctgt ttcccccacc acaaagagcg
     9001 ttttgccgta ctccacgcac ttttccataa aaagcgggat attcccggac aggtatatgc
     9061 ggctcagctc cccgtctttt gattccacct tttctgtacc ctgcacccgg tcataaaacc
     9121 cggcatcgat atacgactga tgggtgcgct ggaagtagtc cggcttacgc agggtgattg
     9181 agatggtgtt acgctcacag gccgggggca ccactatctg gcagcgttct cctgtaggga
     9241 gcgtggccga gaggataggc gacgtttcat caatggtgtc gttgttccac acggccagcg
     9301 ccctggcaaa ggacgagcac tggcgcagcg tgaccggcga ggcgtgcatt tcccacgccc
     9361 cgttgatttt tgtgtgcagc tctccggggc ggttgatggc aatttccgtc agcccctccc
     9421 ggctcaggta gtccccgaac aacagatcgc gcaggtgtga cagcgcgacg ttgccctctg
     9481 acggggtatc agtcagttta tcgtccatcg cttacctcat gcgcagtctg tagacgtctg
     9541 aaaaatcaat gtccgccccc gtgaccaggc caatcacgtc accctggttg aggtacatcg
     9601 tcggcgggat gttgatgctg ttttccagga gcgtttttgc catttccgcc gtggccgcgc
     9661 gggtgttctc cgtgtagtcc gtgttgcgat ccttgcccgg cgacgagccg gaggccgcag
     9721 cggcaaagtc ctgtaccgtg ccgagcagga gggtattacc tatccgctcc agccagtgat
     9781 tgtctatcca gcccgcgacg ccgttctcgc caagcgcccc cacggcctgg ctgtcaacca
     9841 tcggaataac caggcggccg ggttccggcg tgcgcagctc ggtgatggcg aggaacagac
     9901 gcccctgccc gtttttcagc gtgccggtgc ggtacaggcc gcgcgcctca gtgcctgccg
     9961 ggataaggcg cacggcgttg ctggcgctgt aaatgtcctc gctgataagg cagttaaagc
    10021 gcccgcccac gtccgataca aagcgctgca tcatcgcgca ggggatatgc cggtcggccg
    10081 gaatgtacaa atccggatcg aggccgagcc gcttcacgcc ggtaatgcgc gccagcccgt
    10141 tatcctgcga ctgcccggcg ggtgttgcag agcctggcgc ggacgcgctg cccgcaggtg
    10201 atacgttact gctggacgtg cttttcgggg cgctgcttgc agtggttggg gcgctgtctg
    10261 ccagggcgct gaccttgtca aacgtcaggg ggggcggtga tgcctgcggc ggcgcggcgg
    10321 tatcagtcgg agtagtcttc tcctggccgg ttgtgtctgc cagctgtccc agcgggttca
    10381 gatcccgtcc gaggttattg cgctcgcggg cgggggaggt ggcgtgcgat tccggttcat
    10441 ggctcgcgct ttttccgctg ccgtcaccct tcagtttcgc catcaggtaa tccccgccgg
    10501 tggcgataat gaccagtaac accaggctga tggcggccag cacgagcgag cggcggctgc
    10561 ggcgcttcgc gtgctgcacc acct
//
LOCUS       DADLDT020000129        10487 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000129 DADLDT020000000
VERSION     DADLDT020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.130"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(24..1052)
                     /gene="malI"
                     /locus_tag="JQH22_003410"
     CDS             complement(24..1052)
                     /gene="malI"
                     /locus_tag="JQH22_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFB2207623.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRQMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1227..2819
                     /gene="malX"
                     /locus_tag="JQH22_003411"
     CDS             1227..2819
                     /gene="malX"
                     /locus_tag="JQH22_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFB2207624.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2829..4001
                     /gene="malY"
                     /locus_tag="JQH22_003412"
     CDS             2829..4001
                     /gene="malY"
                     /locus_tag="JQH22_003412"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFB2207625.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4105..5106
                     /gene="add"
                     /locus_tag="JQH22_003413"
     CDS             4105..5106
                     /gene="add"
                     /locus_tag="JQH22_003413"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFB2207626.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5142..6182)
                     /locus_tag="JQH22_003414"
     CDS             complement(5142..6182)
                     /locus_tag="JQH22_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFB2207627.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6425..6550
                     /gene="blr"
                     /locus_tag="JQH22_003415"
     CDS             6425..6550
                     /gene="blr"
                     /locus_tag="JQH22_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFB2207628.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6823..7038
                     /gene="ydgT"
                     /locus_tag="JQH22_003416"
     CDS             6823..7038
                     /gene="ydgT"
                     /locus_tag="JQH22_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFB2207629.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7124..7564
                     /locus_tag="JQH22_003417"
     CDS             7124..7564
                     /locus_tag="JQH22_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFB2207630.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7641..8222
                     /gene="rsxA"
                     /locus_tag="JQH22_003418"
     CDS             7641..8222
                     /gene="rsxA"
                     /locus_tag="JQH22_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFB2207631.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8222..8800
                     /gene="rsxB"
                     /locus_tag="JQH22_003419"
     CDS             8222..8800
                     /gene="rsxB"
                     /locus_tag="JQH22_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFB2207632.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8793..>10487
                     /gene="rsxC"
                     /locus_tag="JQH22_003420"
     CDS             8793..>10487
                     /gene="rsxC"
                     /locus_tag="JQH22_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFB2207633.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2405 a         2708 c         2670 g         2704 t
ORIGIN      
        1 aagtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
       61 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata
      121 ccaagttccc gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt
      181 gtcgcatcgg taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt
      241 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
      301 ttatagcaaa ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
      361 gcggcttgct tctggctgga agtgcactcc aacacccaat cgctgtgaaa cggcaggcca
      421 aattttagta gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt
      481 tgccctccca gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
      541 tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg
      601 gaagcgaaaa tcaccgggat agctttttct tctgccatct gccgcaggtc atcgctgctt
      661 cctgcagccc cggcaattac cacaccatcg acaccctgat tcagtaacag tgaaaaccgc
      721 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct
      781 tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg
      841 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg tcgattgcgc
      901 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
      961 cctttgccat taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     1021 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     1081 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     1141 tttatgactc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     1201 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     1261 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     1321 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc
     1381 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     1441 ttgcttttag tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     1501 aaaataaagg cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     1561 taaacttctg gttgaccaat aaaggcattc tgccaaccac agatgccgcc gttctgaaag
     1621 ccaataacat ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     1681 tgatcgccgg tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg
     1741 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg
     1801 gccttgtcgg cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct
     1861 tgggccatat gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     1921 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     1981 caggcggcac gcaggaggtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     2041 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc
     2101 aaggtaaaat gcctgcgttt ctcggcggtc taccaggtgc agcgttagct atgtatcact
     2161 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct
     2221 gcgtcgttgg cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     2281 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     2341 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     2401 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     2461 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     2521 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     2581 ttcctgcaat cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     2641 ttacccgtct gcgtttgtct gtggaagata tgtcgcttgt taatgtgcag gcactgaagg
     2701 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     2761 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     2821 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt
     2881 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     2941 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     3001 tatttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     3061 ccacccagca ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct
     3121 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     3181 cgcccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     3241 ttgctttaga gaagcaggct gacggctggt tttgcgatat gggcaagctg gaagccgttt
     3301 tggcgaaacc agaatgtaaa attatgctcc tgtgtaaccc acagaatcct accgggaaag
     3361 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg
     3421 ttatttccga tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga
     3481 gtaatgtggc gcggggagac tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc
     3541 ccgcgctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     3601 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata
     3661 tcgccgccta tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     3721 acctgacgta tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc
     3781 cgcaatccac ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt
     3841 tgcaaaaagc gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg
     3901 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag
     3961 gtgtggctgg attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa
     4021 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     4081 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     4141 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     4201 atctcgcttc ctgcacaatc ccttgaaaca ctgattcccc acgttcaggt cattgccaac
     4261 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     4321 cttgatgcct gccgccgcgt ggcatttgaa aacattgaag atgcagcccg taacggcctg
     4381 cgctatgtcg agctgcgttt ttcaccaggc tacatggcaa tggcacataa gctgcctgta
     4441 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     4501 caggcgaagc ttatcggcat tatgagccga accttcggcg aagccgcctg tcagcaagag
     4561 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc tggtgatgaa
     4621 cttggattcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     4681 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     4741 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
     4801 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
     4861 agcaccgtag cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt
     4921 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
     4981 gttgccgcgc cagctgctgg gttatcccgt gagcaaatcc gccaggcaca gattaatggt
     5041 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
     5101 aagtaacaaa aatggatggt gcaaattgca ccatccattt ttcacgcaag gcacaacgtc
     5161 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctgg
     5221 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatcgc cgcatagtaa
     5281 gccggataat tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg
     5341 cgggtaagta cgccatcacg catatcgtag ccccagtctt cctgcggcag acgctcgcca
     5401 tttttcagac gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
     5461 tgcacgatat accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc
     5521 cgctgtggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
     5581 tgtgccaaat ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga
     5641 agatgcggtg ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga
     5701 tcgcgcacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc
     5761 acgccttccg cgagcaaacc ttttagcgtc aagaaatcgc tatcccaacg acggttatgg
     5821 aatacagaca gcacacgccc caggcttttt gccagcgcat ccagctctcg cgcttgtgac
     5881 agtgtcacgg taaagggttt atcaacgacc acatatttac ccgcctcaag cgccgctttg
     5941 gctaacggga aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg
     6001 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
     6061 gtttcatcac tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
     6121 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
     6181 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca
     6241 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
     6301 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
     6361 attattgcta agtaacattt accccctgaa gttaatggat caatcaagag agatgtgggc
     6421 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
     6481 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca
     6541 acacaagtaa gctctgcact tgtggagcga catgctgccc gtccgggtgc atgttttcac
     6601 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg
     6661 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
     6721 gcattcttat tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa
     6781 taatacctac ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac
     6841 ttattaaaat ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt
     6901 aattacaccc tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt
     6961 cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc
     7021 tggcactatg tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc
     7081 taaaattaga tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc
     7141 acaacgtatt ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttgag
     7201 tacgacgctg gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac
     7261 gctcggcgga caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat
     7321 cgccatgtgg tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt
     7381 tcctaaacac tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc
     7441 tttttcaccc gtggaagttg gcatagccgt tcgccagttg ctgtttactt tgttggcaac
     7501 cgcattgatc gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc
     7561 gtaataacct tacagttaac ctgttgccgc ctgctctgga ttaacggata ataggcggct
     7621 tttttatttc aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg
     7681 tcaataactt tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa
     7741 aactggaaac cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctgacgtcta
     7801 tttgcgcctg gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca
     7861 ccctggcatt tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca
     7921 aaaccagccc ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact
     7981 gtgcagtgct cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg
     8041 cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca
     8101 tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt
     8161 taattaccgc aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt
     8221 aatgaatgct atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc
     8281 cattctgggt tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat
     8341 tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta
     8401 cgcggaagcc atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc
     8461 tgtgatgcta aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc
     8521 gcaagagcta acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg
     8581 cactaaatgt attcaggctt gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca
     8641 tacggtaatg agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca
     8701 ctgcatctcg ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac
     8761 cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa
     8821 aaaataaaat ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagacca
     8881 acggtacccc cctgcgccag gtactactgg cgcagcattt tgttattcca ctgaaacagc
     8941 atattggcgc tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc
     9001 cgcttacccg tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta
     9061 cggctattgc gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta
     9121 ttgatgccga tggtgaagac tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc
     9181 gcagtcgcga agagttaatc gagcgcatac atcagtttgg tgttgccggg ctgggcggtg
     9241 ccggattccc gacaggcgtt aaattgcagg gtggtggaga taagattgaa acgttgatta
     9301 tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg
     9361 cggctcaggt cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc
     9421 ttatcggcat tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg
     9481 actctaacga tatttctctg cgggtgattc caaccaaata tccttctggc ggtgctaaac
     9541 aattaaccta cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg
     9601 gcgtattaat gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg
     9661 agccgattac cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg
     9721 tctgggcacg gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct
     9781 ctgccgatca aatggtgatt atgggtggcc cgctaatggg ctttaccttg ccatggctgg
     9841 atgtcccggt cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg
     9901 aaccacagga agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg
     9961 atcttttgcc gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca
    10021 cgcataacat tgccgactgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata
    10081 ttcccttggt gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag
    10141 aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg
    10201 aaaaagcgac tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata
    10261 aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc
    10321 ctattgtgat taaagcgggc gaacgcccgg ataacagtgc aattattgca gaacgggaag
    10381 cccgtaaagc gcaagccaga gcgaaacagg cagaactgga gcaaactaac gacgcagcaa
    10441 ccgttgctga accacgtaaa actgccgttg aagcagctat cgcccgc
//
LOCUS       DADLDT020000130        10294 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000130 DADLDT020000000
VERSION     DADLDT020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.131"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..401
                     /locus_tag="JQH22_003421"
                     /pseudo
     CDS             1..401
                     /locus_tag="JQH22_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JQH22_003422"
     CDS             484..741
                     /locus_tag="JQH22_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207634.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JQH22_003423"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JQH22_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFB2207635.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JQH22_003424"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JQH22_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFB2207636.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JQH22_003425"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JQH22_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFB2207637.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JQH22_003426"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JQH22_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFB2207638.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JQH22_003427"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JQH22_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HFB2207639.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JQH22_003428"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JQH22_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HFB2207640.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="JQH22_003429"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="JQH22_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFB2207641.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="JQH22_003430"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="JQH22_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="JQH22_003431"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JQH22_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JQH22_003432"
     CDS             9762..10019
                     /locus_tag="JQH22_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HFB2207642.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2734 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DADLDT020000131        10161 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000131 DADLDT020000000
VERSION     DADLDT020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.132"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..814)
                     /gene="mltD"
                     /locus_tag="JQH22_003433"
     CDS             complement(<1..814)
                     /gene="mltD"
                     /locus_tag="JQH22_003433"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFB2207643.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEM"
     gene            complement(886..1641)
                     /gene="gloB"
                     /locus_tag="JQH22_003434"
     CDS             complement(886..1641)
                     /gene="gloB"
                     /locus_tag="JQH22_003434"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFB2207644.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            1675..2397
                     /locus_tag="JQH22_003435"
     CDS             1675..2397
                     /locus_tag="JQH22_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFB2207645.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(2394..2861)
                     /gene="rnhA"
                     /locus_tag="JQH22_003436"
     CDS             complement(2394..2861)
                     /gene="rnhA"
                     /locus_tag="JQH22_003436"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFB2207646.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            2926..3657
                     /gene="dnaQ"
                     /locus_tag="JQH22_003437"
     CDS             2926..3657
                     /gene="dnaQ"
                     /locus_tag="JQH22_003437"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFB2207647.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            3790..3866
                     /locus_tag="JQH22_003438"
     tRNA            3790..3866
                     /locus_tag="JQH22_003438"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3824..3826,aa:Asp,seq:gtc)
     gene            complement(3997..5043)
                     /locus_tag="JQH22_003439"
     CDS             complement(3997..5043)
                     /locus_tag="JQH22_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFB2207648.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(5054..6039)
                     /gene="tssG"
                     /locus_tag="JQH22_003440"
                     /pseudo
     CDS             complement(5054..6039)
                     /gene="tssG"
                     /locus_tag="JQH22_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(6036..7919)
                     /gene="tssF"
                     /locus_tag="JQH22_003441"
     CDS             complement(6036..7919)
                     /gene="tssF"
                     /locus_tag="JQH22_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFB2207649.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(7935..8429)
                     /gene="tssE"
                     /locus_tag="JQH22_003442"
     CDS             complement(7935..8429)
                     /gene="tssE"
                     /locus_tag="JQH22_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFB2207650.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(8426..9256)
                     /locus_tag="JQH22_003443"
                     /pseudo
     CDS             complement(8426..9256)
                     /locus_tag="JQH22_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(9243..10157)
                     /gene="tagK"
                     /locus_tag="JQH22_003444"
                     /pseudo
     CDS             complement(9243..10157)
                     /gene="tagK"
                     /locus_tag="JQH22_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         2285 a         2685 c         2768 g         2423 t
ORIGIN      
        1 ccatttcaac cgggctactc aggtgcacac gcgccagagc acggctttca tcggtcgttg
       61 gcagacgtac gccataacgc ttgctgtttt tgagaatatc actcaatgcc agcattttag
      121 gcacgtactg cttcgtttcc tgcggcaacg gtaacgacca gaagtccgtg gatttcccac
      181 gcgctttgtt cgttttaatt gccttcatga cccgaccttc gccgctgtta taagccgcta
      241 cggtcagaag ccagtcgtcg tcaaacattt tgttcagacg ctgcatcata ttcagcgcgg
      301 cagttgttga agcaacaaca tcgcgacgcg cgtcataatt gcgggtctgt ttcaaaccat
      361 aattgcgccc cgtgctcgga atgatctgcc agatgcctgc ggcattggcg ccagacgttg
      421 cgtgaggatc aaaagcgctc tccactatgg gtagtagtac cagttccata ggcatgttac
      481 gttttttaac ttgccctgct atccagtaca tatacggctc tgcccgtaaa gttacatcgt
      541 ggagatagct cttattgcgt aaatatttct gtttctgttc gcgaatccgg tcattttccg
      601 gaattcccat ctttagctcg tcgccaatga aagcccacaa gtcaccatct ggcgcgatag
      661 acgtcccatc gtccatccat cgtgcctgac ttgtaaactt tgctgcttcc ccttgaccag
      721 ctgcagaaag gctctgtgcg tgctgttgaa cgttgccggt actctggcaa cccacgagca
      781 ggacagaggc gagtaatatc gcttttgcct tcatgtgtgt gtcaatagtt gcttaaaaga
      841 cgaccgatca taacggcgaa cggagccgat gacaagaaag ttttatcaga acctatcttt
      901 ctttgacctt aaccatgcaa aacgctcttc aggttgttgc aataatgttt cttcattaat
      961 tccattaatt aaatcaatat cttccgttct taaaaaaaca ttaatttgcc gctcattttt
     1021 cagaattacg ggtagtgtta tttgattttt tgcccgtaac tccttaactt tacgataata
     1081 atcatttatg gacaaatcgt gcggaaaaat actcaaagca aacttcatat ttgataacgt
     1141 atattcatga gcacaacata ccaatgtatc gtcaggtaac gcacttagct ttttaagtga
     1201 ttgatacatt tgtaaagctg tcccttcaaa caaccgacca cacccaccag aaaacagcgt
     1261 gtcgccgcaa aatagataag gtttactgaa gtaacagata tgtcctaaag tgtgacccgg
     1321 tgtggtaatt acactaaatt catgccctaa aacgaaggca gtttcgccat ctttgactac
     1381 ctgtgttgtt cccttatctt gtgtctcttg tggaccatac accacaattt gcggaaactt
     1441 ttccaccagt tcttttacgc cgccaacgtg atcgtggtga tggtgggtta gaaatatggc
     1501 ctccggttgc cagttattgg cggtaatggc gtttaatact ggctctgcgt ctccgggatc
     1561 gacaatcagg cagcgacctg cctcatcatt caaaacccag atgtaattgt catcaaaggc
     1621 gggaatactg ttaagattca tagattacct ctcagtgtaa aacggaaggt tgtgatgaaa
     1681 ccggcaagag tccctcaaac tgtcgtggct cctgattgct ggggcgattt gccctgggga
     1741 gagctttatc gcaaggcgct ggagcgccag ctcaacccgt ggttcactaa aatgtatggt
     1801 tttcatctgc ttaagattgg caatttaagc gcagaaatca attgcgaagc gtgcgcggtt
     1861 tctcatcaag tgaatgtttc tgcgcaagga atgcccgtcc aggtgcaggc ggatccactt
     1921 catcttcctt ttgccgataa atccgttgat atttgtctac tggcacatac cttgccgtgg
     1981 tgcaccgatc cgcatcgttt attacgtgaa gccgatcggg tactgattga tgatggctgg
     2041 ctggtgatta gtggcttcaa ccccatcagt ttaatgggat tacgcaaact tgtgccggta
     2101 ttgcgcaaaa cctcgcccta taatagccgg atgtttactc tgatgcggca actggactgg
     2161 ctctctttgt tgaattttga agtgctgcac gccagccgtt tccacgttct cccgtggaac
     2221 aaacacggag gaaaactatt gaatgcgcat attcctgcgc ttggttgctt acaacttatt
     2281 gttgcccgga aacggactat tcctttaacg ctaaatccga tgaaacagag taaaaacaag
     2341 ccacgaattc gccaggcggt tggagccacc cggcaatgtc gtaaaccaca ggcttaaact
     2401 tcgacttggt agcccgtatc ttccagtgtg ggattcatcg ccgcggcacg agccagttca
     2461 tcacagcgtt cgttttccgg gtgtccggca tggcctttaa cccattccca tttgatttga
     2521 tgctgcccca atgcagcatc aagacgttgc cagagatcga cattttttac tggttttttg
     2581 tctgctgttt tccagccacg ttttttccag ttatggatcc actgggtgat accctggcgg
     2641 acatactggc tgtcggtact caaaatgact tcgcaatgtt cttttaacgc ctccagcgcg
     2701 acaatagcgg ccatcaactc catacggttg ttggtggtgc gggtgtagcc tgcgctaaag
     2761 gttttctcgc gtccgcgata gcgtaaaata gcgccgtaac ccccaggtcc aggattaccc
     2821 agacacgaac catcggtgaa aatttctacc tgtttaagca tctctggtag acttcctgta
     2881 attgaatcga actgtaaaac gacaagtctg acataaatga ccgctatgag cactgcaatt
     2941 acacgccaga tcgttctcga taccgaaacc accggtatga accagattgg tgcacactat
     3001 gaaggccaca agatcattga gattggtgct gttgaagtag tgaaccgtcg cctgacgggc
     3061 aataacttcc atgtttatct caaacccgat cggctggtgg atccggaagc ctttggcgta
     3121 catggtattg ctgatgaatt tttgctcgat aagcccacgt ttgccgaagt agccgatgag
     3181 tttatggact atatccgcgg cgcggagttg gtgatccata acgcagtgtt cgatataggc
     3241 tttatggact acgagttttc gttgcttaag cgcgatattc cgaagaccaa taccttctgt
     3301 aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc ccggtaaacg caacagcctc
     3361 gatgcgctat gtgcccgcta cgaaatagat aacagtaaac gaacgctgca cggggcatta
     3421 ctcgatgccc agatccttgc ggaagtttat ctggcgatga ccggtggtca aacgtcgatg
     3481 gcttttgcga tggaaggaga gacacagcag caacaaggtg aagcaacaat tcagcgcatt
     3541 gtacgtcagg caagtaagtt acgcgttgtt tttgcgacag atgaagagct ggcagcacat
     3601 gaagcccgtc tcgatctggt gcagaagaaa ggcggaagct gcctctggcg ggcataaata
     3661 catgtaaaag gcgctaaaaa tagcgacttg ggcgattttt gcagcaaacg attcaaaaga
     3721 tgagaaaaat cgttgacgaa ggtcgaggca atccgtaata ttcgcctcgt tcccaacgga
     3781 acacaacgcg gagcggtagt tcagtcggtt agaatacctg cctgtcacgc agggggtcgc
     3841 gggttcgagt cccgtccgtt ccgccaatat tcagaaagcc cgaatcagta atgattcggg
     3901 ctttcgtcgt tttggtgaag tgagaatttt tcactccgaa gtaaaacgga tatgatttaa
     3961 aaaatggcca gacattataa attctggcca cttctgttac tccagcgcat cccatttctc
     4021 cagcagttct actggaattt catttattac ctccgagacg cgttttccga ccaggcgttc
     4081 tgcctggcgc agcaaaggaa tggtcgggct gctcggttcg ctgtgctcaa accagcgacg
     4141 gatgacacgc aggcgttcca gcgcgtcgtt tcggtcgcgg atctgccggg gttctgcgcc
     4201 ggtcatcggt gatatttcct ccgcgatatg caccggtagc gcaatctgct ctgcctgcat
     4261 ctgttcagta tgaacgatgg tggcgttttc cggctgctca gcctgtggtt gtggcaggat
     4321 ctccggcgtg gtgctctgca ctgctcccgg taacagggcc agcagttgcg tcagacggga
     4381 gaagtccggt gcctggtcat tcagcgtttc acgcgcatgg cgttgcagcc gctcagtggt
     4441 ctctgctgcc tggcgaaagg catccagcgg cagtgtgccg cgcgcttcca ggtctgccag
     4501 ttgctggcgt accgattctg gtgccagcgc atccgccggg cgtaatgcag atagcgaacg
     4561 ctccacatcc cgcacctgaa ggcgcatggc agcattattt gacagtgtaa tgccacgaat
     4621 atccgccatt acaccttcgt ggtccagcaa cgccgccagc gcgttactgc gtgccaccgc
     4681 agcatcttcg gcggtgatat cttcagtcgc gagcagttga ggatggatgg cgtccgggta
     4741 cgtggagcac aacgtttcca gcagcgtgag cgcctccgac agtcccctgg caccggcctg
     4801 ctggatacgg cagcgcagca ggacaaccag aatgcggata tctttgctgc gcatcagcag
     4861 acgatgggcg tcccgctcaa gctccgccca gttaatgttt tccggcgtac tgacaaaatc
     4921 gccatattgc gcttcttcac gcggtgcggc gcgggtgaac agcagcaggt attccggatc
     4981 gtactccaga tccgggccgc aggggcattc cgccgaaatt gggtcagaca ggaatatttc
     5041 catttaatta tcctcagtgc gccaggtact gttctggttc gaaaatcatg cccgtcaccg
     5101 ggttgtcgtg catggcttta cccgcccatg cagtccaacc cagttggtac ggaccaccgg
     5161 taacagccgg aggggcggcg tggggcttta gctgtagctc aatttcccag cagtattcat
     5221 aaccactaaa ggcgcggacc agttctgtca ggacgggcag gttattgctg ccaggcagaa
     5281 aacgtcggta atcctccagc gtcagcgggc cgataaccag ccggaactta tactgcatgt
     5341 ccggcacggc ctggccgatg agtgcaccat tgccaataac ggaagagggg gcaggcatcc
     5401 ccagccgggt gatctcatcg tccgccacgg ctatccagtg caggacatac tcctgtatcc
     5461 ggaaaggtac accgaaatag tgcgccagcg tggtggccag accatccgga ttgcgggact
     5521 cacgcaccag atgggccgat gccgccagac gagcgtgatc tggcaacggg ctgtcagcgc
     5581 tctcccgcaa atcctgcccg ctgaggctgg cgacataaaa ggcaaagcgg tcatgttgcg
     5641 gtttatccag accgccccca gcggactggg cgctgcgcca tgcctggtaa aactgcgtca
     5701 gccagcggtg atgaaaaaga tcagaaaaac gggtcagcgt cggctttcag tccggttacg
     5761 cgccagttcg gtgtagtgca gcggcagtgg accgttcggc ccccacagcc cgaggctgta
     5821 cagactgatg tgcaggcgcc cttcctgcca gcccacactg gcgatttctc gtggcgcaaa
     5881 ggtcagcgcg ggcgtttgtc cgaggcgaaa tttttccatc cgtggttgcc aggtgttgcc
     5941 cgccggaacg gtacacaact gcacatccac gcggcgcatc aggttcagaa aaccgtaacg
     6001 ccaggggggg gcctgcgcct gagtgaaatc gttgctcata cgcttccccg ctgtcccgcc
     6061 ctgacaggcc aggtcataat tttgccgcgc tgcatgctgt gcagcgtcat ctgcgagaag
     6121 gtattaatgg aaacatggcg ggcgatatag tgttccagca ccaggccgaa cagatacgga
     6181 ctgatgccgg aaaaaccttc ttcatccacc gtcagttcac aactgacgcc acgcccatag
     6241 accagtagtc cggaaccggg cagacggcgg gtgaccgggg tcgttttaca accaatcagg
     6301 ctgcgcacct ggcgcgattg cggactgtca tgagcaggaa taaacaggtt cagcagatct
     6361 cgcaacgcct ggccgccggt acggtgatcc agatcggcca gcggcagata attaaaagat
     6421 aactggcgga tcaaccgcca tgccatctca cgttcagcca gcgggggctg cggcgagcgt
     6481 ggcgggcgga taagccctac gcccgccacc gggatggctg catccacggt tagatcgtcc
     6541 cggccattgc gggcaataag acagggcaga tcgcggttag tgaccatggc cgtgatggtg
     6601 atgtggcgca gattttccgg gtacggcgct tcatactggt caaccagcga caggaacact
     6661 tccgagcccg tatagggggt acgggtgccg taacggcggg cgttttctga taagcggcgg
     6721 ggctcccggc gcagggaaaa ataacgtccg tgattgcctt catcattatt gcgggtgtgg
     6781 tagagcgggc gaaaggccat cttccgtgtg gtgtcggttt ccagcccttc cacttcctgg
     6841 accgagaaca cctcataatc cagcggatgg gttctgtcca ccaccagatg ctgttccgtc
     6901 gtgctgtgcg tgacatcgat ccgggcggtg acgcggggaa acaggttgat aaccggggta
     6961 cagaacaggc tgaactgtgc cgcatttgtc tggtggatca gccagtccgg cggcaggcgg
     7021 ttcagcagga taacaatctc cgccacgcca ccgtcaattt tctgtaatcc ggcagacagg
     7081 cctgtcgggg tgaaaaaata gaagcgttcc ggacaggcaa aatattcatg cagcaggtta
     7141 tgaccgtgaa acacgttcca cgccagcggc agcagcccct gatccggctc caggccttcg
     7201 tacataacgg gctgttgcag attcacatcc agcgcgccgt caaaatgccc cggaatgcca
     7261 gccagggtcg cgatggcgct ggtatgtaga agttctaaca ggtgtgaggc cgtacgctcc
     7321 tcgccgcaga gataaaacgg cagcctgtcc agtccggcaa gctgactgaa cgttagttca
     7381 ccaaaggtgc gcagggtaat acgcagcgcc ccggcgacgt gaatgttggc cggcaggtag
     7441 cggtgcaggg caggcatatc cggtggcgcg gcggtcagtc gcgcctcttc gatggcgagc
     7501 ggccacagcg ttacatcctg gctgctgcga aactggcagg cagtgctttc gccttccgga
     7561 atggcggaga aaaaggctgt gtcgcgcggg acggtaaatc ctttcgccag atcgccttct
     7621 tccgtatcgg gatgcaactg cgccacgctc attgagggcg tgggtgtgac gtaattgggg
     7681 ctgaccactt ccagcaggcg ctgggtgaaa cgggggaatt cagcgtcaat ttttagctgc
     7741 gtgcgggcgg tcaggaagct gaaggcctcg accattcgct ctacatacgg atcggcaata
     7801 tccgttccct gcatgcccag ccgggcggcg actttgggat gacgggcggc aaattcggct
     7861 cccgtttccc gcagatagct cagttcgcgg ttgtagtatt ccagtagccg tggatccatc
     7921 ggtctctctc tgaattaaaa aaacgtaatg cgactctgct ccatatccag cgcgctacgt
     7981 acccggaatg cagtcggata cggctgcgtg aggatctcgc cgcgaatttc aaactgtaat
     8041 gtgttatggc cactctggcg gcgctcatcc tgaagtggga tgatttgcag agtggacgct
     8101 ttcagccggg gttcaaaccg ggtgatggtt cgcaaaatgg ccctgcggat atcgtcccac
     8161 ttgtgctcat acagaaagct gcccgccagt ggcggcaggc cgtagttaag tacggacgct
     8221 gccgcgtacg gataacgtgc agcatcaata tcatcttcat ggctgacggt gttgagcaga
     8281 aatgacaaat cccggcggat gatctcctta atctgaaccg gactgacgct gataccgtgg
     8341 tcgcgtttct ggtttggggc gtcgtcacac aggcggtcaa acagcgtggg cagcaggtga
     8401 ttgcctggac gctggcggga agcgctcacg cgtcgcttcc ttcagcggtg ttaaaggtac
     8461 aggtggacaa atccagcagg ctgatatcgc cgtgaccggt gagccacacc ctctgaccca
     8521 gcgcctgtac cagtgtctct cccggaccgt cctgccagac cgtttcccgg cacagacgca
     8581 gggagtcggc agccttttca gagccgctgt agcggggaaa cagctaggca ctgtgtgtat
     8641 cgccattgtt cagggtgacg ttgacgggtt tccagatcaa atcgatcaga tgcgcaggcc
     8701 gcggcgattt cagcgaacag atttgtgaaa acggcagcca gatatatgct ccgcccgcga
     8761 tcagttcgag taccggaccg gtacgggagt cgctgtcgct aatccagtca aatgcgccgc
     8821 cattccactg cccggaagtg tctgttatgg cctcaagcgc gcggctgcgg tgagcatcag
     8881 cttcctgcgc ttcaccacgg gcgttacagg cgagggcggt cagcagatcc tccatccagg
     8941 cgggcggcgg gagaataaca ccgggccgct gttcgcccta aaagcaggcg tggcggtata
     9001 tctcacagcg gatcagctcg ccaaatacct gcgcttcgcg agtataattt gcgtccatcc
     9061 gggcgcatag ctggatctgt tgcagtgccc gcgaccagtc gcctgccacg cacaataact
     9121 gaaacaggct gtggcggcgg gaggcctgcg ctgggtgcat cctgacctgt tgctctgcca
     9181 tctgaatgcc ctccaccacg gaataatccc gcagcagcgc cgacaaggta atcggtagct
     9241 cgttattgtt tgtcatgata tatctctcca tgttgtgccg ggtttatccg gtaatggctg
     9301 tcaatgccaa tgatgcgatg ctctttccgt gtcagatctg gcagtacggt gttatgcgct
     9361 gtttccgggg cgtattccgg ggaaagtaaa tgcaggatat cagggggagt cttttcgaca
     9421 ggccagatag tttcatcttc gccagtggca tccagtacat tgagaatagc gtcaatgctt
     9481 aacccaccag tgaccatatc ctgcaatgtc tcggcttcgg tgaccaggtc acgcaatgaa
     9541 tccagatcct gcgaggaggg ctcttcgtta aaaggaaaag gatcagcatg gcgattaaga
     9601 tcttgttcgg gagataacaa cgcctcgcgg tattcctgat aaagtcgggt cagggcattt
     9661 cctttttccg caccttctcc ggtggaggag gctccatgaa caggaatgat atcgaatgga
     9721 ttttgtgatg tcagatgctg ctgcctgaac caatccagat ccagatattc ggttattgct
     9781 tccgacgtga tggcagtctg cgatatcgtt tttactgtgc tcaggttcgg agatgatgga
     9841 ttgccatgta caaggcacta tgaagataac ccccattcta tcaggtcgcc ttcattcaga
     9901 cgcatacggt gatgaggttc cacaatctgg tcgtttaccg tacagcaata ttcggtagac
     9961 tgattgataa tccaccatgc ttcttcgtgg cgggtcagag tgagggcaat ctttacatca
    10021 tcattcacct taaaaacagg aatccaggga gaggtaacgg tgaagataat ttgtgttcct
    10081 tcagtcgcgt aaattgtgtt atcggcgatc ccgtgcgcct gtattttttg gagtccccac
    10141 gtaatatttg gtttcattgt g
//
LOCUS       DADLDT020000132        10140 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000132 DADLDT020000000
VERSION     DADLDT020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.133"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            158..289
                     /locus_tag="JQH22_003445"
     CDS             158..289
                     /locus_tag="JQH22_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HFB2207651.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            549..1544
                     /gene="pgl"
                     /locus_tag="JQH22_003446"
     CDS             549..1544
                     /gene="pgl"
                     /locus_tag="JQH22_003446"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFB2207652.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1585..2538)
                     /locus_tag="JQH22_003447"
                     /pseudo
     CDS             complement(1585..2538)
                     /locus_tag="JQH22_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2722..3774
                     /locus_tag="JQH22_003448"
     CDS             2722..3774
                     /locus_tag="JQH22_003448"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFB2207653.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3850..5283
                     /locus_tag="JQH22_003449"
     CDS             3850..5283
                     /locus_tag="JQH22_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFB2207654.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5466..7646
                     /locus_tag="JQH22_003450"
     CDS             5466..7646
                     /locus_tag="JQH22_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFB2207655.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7787..9070)
                     /locus_tag="JQH22_003451"
     CDS             complement(7787..9070)
                     /locus_tag="JQH22_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFB2207656.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9205..10086)
                     /locus_tag="JQH22_003452"
     CDS             complement(9205..10086)
                     /locus_tag="JQH22_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2207657.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2466 a         2367 c         2554 g         2753 t
ORIGIN      
        1 tttagtaatg atcaaaatca tatctcaatg tactgttttt ttctttaaaa aaacagtaca
       61 ttctatttta taatgactgt gtattcccat ggaaggagat cacccaattc tcccccccga
      121 cttttgacgc agtgtcttgt taacaggggg attgaccatg cttattggct atgtacgcgt
      181 atcaacaaat gaccagaata ccgatctaca acgtaatgcg tttatttgtg ccggaagtga
      241 gttgattgtt gcgcgaatgg gaagttggcc agaagttgtt atggcatagt gtttacggtc
      301 gttgatacag gtaaacaaaa gcggggagag tcatactccc tgaatagtcg cgaaaatatc
      361 tgtggcattg tcaaattcgt gtcggtgact aatattttag taatatgcca ttcgaaatgg
      421 agtgctaact tattgattta attgatgtgc gatattgtat tttatcgtgt tttgttgtct
      481 gttttttatc cagtaagtgt tgttatatgt atgaaagata agggatttat catttttgag
      541 gtgtgttcat gaagcaaaca gtttatatcg ccagccctga gagccagcaa attcacgtct
      601 ggaacctgaa tcatgaaggc gcactgacgc tgacacaggt tgtcgatgtg ccggggcagg
      661 tgcagccgat ggtggtcagc ccggacaaac gttatctcta tgttggtgtt cgccctgagt
      721 ttcgcgtcct ggcgtatcgt attgccccgg acgatggcgc actgaccttt gccgcagagt
      781 ctgtgctgcc gggtagtccg acgcatattt ccaccgatca ccaggggcag tttgtctttg
      841 taggttctta caatgcgggt aacgtgagcg taacgcgtct ggaagatggc ctgccagtgg
      901 gcgtcgtcga tgtagtcgag gggctggacg gttgccattc cgccaatatc tcaccggaca
      961 accgtacgct gtgggttccg gcattaaagc aggatcgcat ttgcctgttt acggtcagcg
     1021 atgatggtca tctggtggcg caggaccctg cggaagtgac taccgttgaa ggggccggcc
     1081 cgcgtcatat ggtattccat ccaaacgaac aatatgcgta ttgcgtcaat gagttaaaca
     1141 gctcagtgga tgtctgggaa ctgaaagatc cgcacggcaa tatcgaatgt gtccagacgc
     1201 tggatatgat gccggaaaac ttctccgaca cccgttgggc ggctgatatt catatcaccc
     1261 cggatggtcg ccatttatac gcctgcgacc gtaccgccag tctgattacc gttttcagcg
     1321 tttcggaaga tggcagcgtg ttgagtaaag aaggcttcca gccaacggaa acccagccgc
     1381 gcggcttcaa tgttgattac agcggcaagt atctgattgc cgctgggcaa aaatctcacc
     1441 acatctcggt atacgaaatt gttggcgagc aggggctact gcatgaaaaa ggccgctatg
     1501 cggtcgggca gggaccaatg tgggtggtgg ttaacgcaca ctaaccgctg atttaaccgg
     1561 cgcagtctct cctgcgccgg tgtattaact tatctcctgt aacgcatgtc tctggcgttc
     1621 gacgatattg gtccacaaat tgtctttatc gtcagtccac aaattaatca gcaaggaaaa
     1681 aaagcgttca gctgccggag aaagcacggc atctttgcgg cgaataatcc ccaatgtccg
     1741 acgtataacg ggttccacca gtgggatacc aataatcgaa gaatagggcg catggggcat
     1801 tgccagacca ggaagcgccg aaatccccag tcctgcctcc actagtccta atgacgtcga
     1861 aagatgacgc acttcgtaaa accagtccag cttccagggc ttgtcggcta gctgttgttc
     1921 tatcaacaat cggttgccgc tggaggaacg tacgccaatc attttgtaac ccactaactc
     1981 ctgccattct acaagctact ttttggccaa tggatggtca cgccgacagg ccagcacgaa
     2041 tggctcgttg accagtgggg taaaatcaat ggatgaattt gtgacgttgt tcatgtttat
     2101 gccaaagtct gattcgttac acagcaccga ttccatgcaa ttattcgtgc cttgttccag
     2161 aatccgcact ttaatattgg gatacagctc attaaattta ccgattgcca gcggtaaaaa
     2221 ataaaatact gcggtcggaa tacacgctaa tgtcaccata tcacgatgat aagcattcat
     2281 atcacgaata ttaaaaagcg tttcatcgaa tttctttatt aattcccgcg cctccgggag
     2341 caaccttttt cctgcttttg ttaacgtaac cttgcgagtt gtacgctcaa atagttgaat
     2401 atgtaaatct tcctccatct tttttattct tcgcgttaat gcaggttgcg taatattgag
     2461 taatttagcg gcattgttaa atgaactgga ctctgccagt atgacaaatg ccttcatact
     2521 tgataattca tgcttcatta ttactccgga aaatggaagc gacgattttg gggggctggt
     2581 cgttaaaaat tttaactgca tttagccaac ttaaattaat gaaaaaatgt tattaatcgt
     2641 tgagctaaag tcattagaga tgctttgtcc ttaatgtaac catatcgcaa taagttatgt
     2701 ttttaaattg agggcattat tatgaaaaaa ataccctgcg tgatgatgcg aggtggaacc
     2761 tcgagggccg cgttcctgtt agcggaacat ttacccgaag atcaaacgca gcgcgataaa
     2821 atattgatgg caattatggg ttccggtaac gatctggaaa ttgacggtat tggcggcggt
     2881 aatccactga ccagtaaagt cgccattatt agccgttcca gcgatccgcg tgctgatgtc
     2941 gattatctgt ttgctcaagt tatcgtccat gagcaacgtg tcgataccac gcctaactgc
     3001 ggcaatatgc tgtctggcgt aggggcattc gccattgaaa atggtttgat tgcagcgact
     3061 tcgccagtta cccgtgtacg tatccgcaac gtcaatacgg gtacgttcat cgaagctgat
     3121 gtgcaaacgc caaatggtgt tgtcgagtac gagggtagcg ccagaattga cggcgtaccg
     3181 ggtactgccg caccggttgc gctcactttc ctgaatgccg ctggaacgaa aaccggaaaa
     3241 gttttcccga ctgataatca gattgattat tttgacgatg tcccggtgac ctgtatcgat
     3301 atggcgatgc cagtcgtcat tattccggct gaatatctgg gaaaaacagg ttatgaatta
     3361 ccggcggaac tggatgccga caaagcatta ttagcccgca ttgaatctat ccggctacaa
     3421 gcgggtaaag caatgggctt aggtgatgtc agtaatatgg ttatccctaa acctgtgctt
     3481 atttctccag cgcagaaagg cggggcaatt aatgtgcgtt attttatgcc gcattcttgc
     3541 catcgcgcgc tggcgataac cggtgctatt gctatttcca gtagttgtgc attggaaggc
     3601 accgtcaccc gacaaatcgt cccttctgta ggatatggca atatcaatat tgaacacccc
     3661 agtggtgcgc tcgacgttca tttaagtaat gaaggtcagg atgccacgac gttacgcgca
     3721 tctgttattc ggacaaccag aaaaatattt tccggtgaag tttatcttcc ctgaaaaaat
     3781 tcgttgtcag gataaggaca atcaataaag gacttctgta tgagtcatac agaaagaaca
     3841 ggattttaaa tgaataagaa atcgttatgg aagctaattc tgatattagc gatcccatgt
     3901 attattggtt ttatgccagc tccggcagga ttaagcgaac tggcgtgggt gctttttggt
     3961 atttacctgg cggccattgt gggtctggtt atcaagcctt tcccggaacc tgtcgtactg
     4021 ttaattgccg ttgctgcctc aatggtggtg gtcggtaact tatccgacgg tgcgtttaag
     4081 accaccgccg tattaagcgg ttactcttca ggtactacct ggctggtgtt ctcggcgttt
     4141 accttaagcg ccgcatttgt gaccaccggt ttaggtaaac ttattgccta tctgctgatt
     4201 ggtaaaatcg gtaacaccat gctgggtctg ggttacgtta cggtattcct cgatctggta
     4261 ctggctccgg caacaccgtc taacaccgcg cgtgcgggcg gcattgtgtt accgatcatc
     4321 aacagcgtgg cgatggcttt ggggtccgaa ccggaaaaaa gtccgcgtcg tgtcggacat
     4381 tacctgatga tgtccattta catggtcacc aaaaccacca gctatatgtt ctttaccgca
     4441 atggcgggga acattctggc gctgaaaatg atcaacgaca ttctgcacct gcaaattagc
     4501 tggggtggat gggcgctggc cgccggattg ccgggcatca ttatgctgct ggtcaccccg
     4561 ctggtaattt acaccatgta tccaccagaa attaaaaagg tggataacaa aaccatcgct
     4621 aaagcgggcc ttgccgaact gggaccgatg aaaatccgcg aaaaaatgct gctcggtgtc
     4681 tttgtgctgg cgctactggg ctggattttc agtaagtctc tgggggttga tgaatccact
     4741 gtggcaatcg ttgttatggc gaccatgctg ctgctgggta tcgttacctg ggaagacgtg
     4801 gttaaaaata aaggcggctg gaatacctta atctggtacg gcggtattat cggcttaagc
     4861 tccttattat cgaaagttaa atttttcgaa tggttagctg aagtctttaa aaataacctg
     4921 gcatttgatg gtcacggtaa cgttgctttc ttcgttatta ttttcctcag catcatcgtg
     4981 cgttatttct tcgcttccgg tagtgcctat atcgttgcta tgttacctgt atttgccatg
     5041 ctggcgaacg tctccggcgc accgttaatg ttaaccgcgc tggcactgtt gttctccaac
     5101 tcctatggcg gcatggttac tcactatggc ggcgcggcag gtccggtcat ctttggcgtg
     5161 ggttacaacg atattaaatc ctggtggttg gtcggtgcgg tactgacgat attaaccttc
     5221 ttggtgcata tcaccctcgg cgtgtggtgg tggaatatgc tgatcggctg gaacatgctg
     5281 taaatatact cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc
     5341 acttacttat gtaagctcct ggggattcac tcacttgtcg ccttcctgca actcgaatta
     5401 tttagagtat atccatttat tatctttctg cgcacttcac ggtgcgcaga tatctggagc
     5461 atttgatgat caagttatct gaaaaaggcg tgtttctcgc cagtaataac gaaataattg
     5521 ccgaagaaca tttcaccggc gaaattaaaa aagaagaagc aaaaaaaggc actattgcct
     5581 ggtctattct ctcttctcat aatacgtccg gaaatatgga taaacttaaa attaagtttg
     5641 attcattagc ctctcacgat attacctttg ttggtattgt acagaccgct aaagcgtccg
     5701 gaatggaacg tttcccgctg ccgtatgtgc tgaccaactg ccataactca ctctgcgccg
     5761 tcggcggcac cattaacggt gatgaccatg tttttggttt atcggcagct cagcgttatg
     5821 gcggtatttt tgtgcctccg catattgcgg tcatccatca atatatgcgt gagatgatgg
     5881 ctagcggggg caaaatgatc ctcgggtcag acagccacac ccgttacggt gcattaggga
     5941 caatggcagt cggtgagggt ggcggtgagt tggtaaaaca gctgcttaat gacacctggg
     6001 atatcgacta tccgggcgtg gttgcggtgc atctgaccgg aaaacctgcg ccgtatgtgg
     6061 ggccacagga tgtggcgctg gctatcattg gcgcggtgtt caaaaacggt tacgtcaaaa
     6121 acaaagtcat ggagttcgtt ggaccgggcg ttagcgcgct ctctaccgat ttccgtaaca
     6181 gcgttgacgt gatgaccacg gaaaccacct gtttaagctc cgtctggcaa actgatgaag
     6241 aagtgcataa ctggctggcg ctgcacggtc gcggccagga ttactgccag cttaaccctc
     6301 aaccgatggc gtactacgat ggctgcatca gcgttgattt aagcgccatc aaaccaatga
     6361 ttgcgctgcc gttccacccg agcaacgtgt atgaaatcga cacactgaac cagaacctga
     6421 ccgacattct gcgtgagatt gaaattgagt ccgaacgcgt ggcgcacggt aaagccaaac
     6481 tctcgctgct ggataaagtg gaaaatggtc gcctgaaagt gcagcagggg attatcgcgg
     6541 gctgttctgg cggtaactac gaaaacgtca tcgcggcggc gaatgcactg cgcggtcaat
     6601 cctgtggcaa tgacaccttc tcgctggcgg tttacccgtc atcacagccg gtgtttatgg
     6661 atctcgccaa aaaaggtgtg gtagcagatt tgattggcgc aggcgcaatc atcagaaccg
     6721 cattctgtgg cccatgcttt ggcgcgggcg atacgccaat caacaacggt ttgagtattc
     6781 gccacaccac gcgtaacttc ccgaaccgcg aaggctctaa gccagccaat gggcagatgt
     6841 cagcggtggc gttgatggac gctcgttcta tcgctgcgac tgcggcaaac ggtggctatt
     6901 taacctctgc cagcgaactt gattgctggg acaacgtgcc ggagtacgcc ttcgatgtaa
     6961 cgccgtataa aaaccgtgtt tatcagggct ttgtgaaagg ggcaactcag caaccgctga
     7021 tttacggacc gaacattaaa gactggccgg aattgggtgc gctgactgac aatatcgtcc
     7081 tgaaagtgtg ctcgaagatc ctcgacgaag tcaccaccac cgacgaactg attccttccg
     7141 gtgaaacctc ttcttatcgt tcaaatccga ttggtctggc ggggaatgtc agcgagctga
     7201 cagaggtgtt tgcgcgcatt aagcagattg ctggtcagga gcatattgat ccgctgcaaa
     7261 ctgaaattgg cagcatggtc tatgcggtga aaccaggcga tggttctgcg cgtgaacagg
     7321 cggcgagctg ccagcgtgtg attggcggtc tggcgaatat tgccgaggag tacgcgacta
     7381 aacgctaccg ttctaacgtc atcaactggg ggatgttacc gctgcagatg gcggaagtgc
     7441 caacctttga agtgggggat tacatttaca tccctggcat taaagcggcg ctggataatc
     7501 cgggtacgac gtttaaaggt tatgtgatcc atgaagatgc gccggtaacg gaaattacgc
     7561 tttacatgga aagcctgacg gctgaagagc gcgagattat caaggcgggt agtttgatta
     7621 acttcaataa aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg cattcggcac
     7681 ttattgtcgg atgcgatgct ggcgcatctt atccgaccta caaatcgcat cgaaccgtag
     7741 gccggataag gcgtttacgc cgcatccggc aaatagttaa tagctcttac ttcttcgcct
     7801 ctgcaaccac tttgctgccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag
     7861 tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta ttacccgcaa
     7921 acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa ccttcgttga
     7981 tggcgctatc acggatcacc acctgaccgt tggtattagc atcaacatcc agcgagcggc
     8041 ccagttgcgc cacgccatca ccggaagcat tgaaacggct gtttacggcg aggaaaccgt
     8101 aatagatgtt agacagcgta gccggtgcaa acacatatgc ttcttgctgg gtacgtgagt
     8161 tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca
     8221 catccccttc aatatagctg ttggtcacca gcgtacgcgg ctgacggttg gtttccagac
     8281 ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt
     8341 tgttaatctg tacttggtca ccgtcagtac gcagtgctac cgccggatgg ttacctgcat
     8401 ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagt ccattgtttt
     8461 gtgaccagaa gaccgcagaa cacagaacac cgatactgtc gctgcgtttg ctctggcagc
     8521 tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt
     8581 gacgccagtc ggcagggctc atgcccccat caagggaaag cccaatcttc acatcaatcg
     8641 gtttttcacc tgtaccgtac agagtgattc cacctggagc agcagggaca tacaccgttc
     8701 cctgatactc cccaggcatc acggcaatat actggcgctt gttggtgcgc ttgataattg
     8761 ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtaccc gccgggccga
     8821 caacaaagtc aggttgcgca ggcagggtta tcggggacgg attccacgct gcagcacctg
     8881 gtgtcaggga tgcaaaatag tgttgcgcat cgaaattcag cgcttctttt gccgacagaa
     8941 tcgggcgaga agaggtacca ggcgcagttt gatcagaagg acgttgatcg ggtggtgttg
     9001 agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa
     9061 atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg aagcctgctt
     9121 ttttatacta agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
     9181 tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact ccctgcctct
     9241 gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat gttgagcaaa
     9301 cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact cgtgaaaggt
     9361 aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa aataccttga
     9421 tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt ctccgccaag
     9481 aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat caacatgcaa
     9541 tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa gatatccatc
     9601 tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg taacaacagc
     9661 cagttccatt gcaagtctga gccaacatgg tgatgattct gctgcttgat aaattttcag
     9721 gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc gagtggcagc
     9781 gaccgggttt gttgttatat ggccttcagc tattgcctct cggaatgcat cgctcagtgt
     9841 tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat tgagcattgc
     9901 cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc tccttattgc
     9961 tttaattttg ctcatgtaat ttatgagtgt cttctgcttg attcctctgc tggcgaggat
    10021 tttttcgtag cgatcaagcc atgaatgtaa cgtaacagaa ttatcactgt tgattctcgc
    10081 tgtcagaggc ttgtgtttgt gtcctgaaaa taactcaatg ttggcctgta tagcttcagt
//
LOCUS       DADLDT020000133         9951 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000133 DADLDT020000000
VERSION     DADLDT020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.134"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="JQH22_003453"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="JQH22_003453"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFB2207658.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="JQH22_003454"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="JQH22_003454"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFB2207659.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="JQH22_003455"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="JQH22_003455"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFB2207660.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="JQH22_003456"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="JQH22_003456"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFB2207661.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="JQH22_003457"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="JQH22_003457"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFB2207662.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="JQH22_003458"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="JQH22_003458"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFB2207663.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="JQH22_003459"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="JQH22_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="JQH22_003460"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="JQH22_003460"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFB2207664.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="JQH22_003461"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="JQH22_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFB2207665.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="JQH22_003462"
     CDS             complement(8974..>9951)
                     /locus_tag="JQH22_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFB2207666.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DADLDT020000134         9928 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000134 DADLDT020000000
VERSION     DADLDT020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.135"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..339
                     /locus_tag="JQH22_003463"
     CDS             <1..339
                     /locus_tag="JQH22_003463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207667.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="JQH22_003464"
     CDS             527..1642
                     /locus_tag="JQH22_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207668.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="JQH22_003465"
     CDS             2321..2539
                     /locus_tag="JQH22_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFB2207669.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="JQH22_003466"
     CDS             2757..2999
                     /locus_tag="JQH22_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207670.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="JQH22_003467"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="JQH22_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFB2207671.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="JQH22_003468"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="JQH22_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFB2207672.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="JQH22_003469"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="JQH22_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFB2207673.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="JQH22_003470"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="JQH22_003470"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFB2207674.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="JQH22_003471"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="JQH22_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFB2207675.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2543 g         2500 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 tgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttc
//
LOCUS       DADLDT020000135         9911 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000135 DADLDT020000000
VERSION     DADLDT020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.136"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(165..905)
                     /gene="pflA"
                     /locus_tag="JQH22_003472"
     CDS             complement(165..905)
                     /gene="pflA"
                     /locus_tag="JQH22_003472"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFB2207676.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="JQH22_003473"
     CDS             complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="JQH22_003473"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFB2207677.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3434..4291)
                     /gene="focA"
                     /locus_tag="JQH22_003474"
     CDS             complement(3434..4291)
                     /gene="focA"
                     /locus_tag="JQH22_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFB2207678.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4697..6457)
                     /locus_tag="JQH22_003475"
     CDS             complement(4697..6457)
                     /locus_tag="JQH22_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HFB2207679.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6587..7213
                     /locus_tag="JQH22_003476"
                     /pseudo
     CDS             6587..7213
                     /locus_tag="JQH22_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7412..8500
                     /gene="serC"
                     /locus_tag="JQH22_003477"
     CDS             7412..8500
                     /gene="serC"
                     /locus_tag="JQH22_003477"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFB2207680.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8571..9854
                     /gene="aroA"
                     /locus_tag="JQH22_003478"
     CDS             8571..9854
                     /gene="aroA"
                     /locus_tag="JQH22_003478"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFB2207681.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2369 c         2484 g         2537 t
ORIGIN      
        1 cggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta tctcaggtac
       61 gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat gaagcgcgga
      121 ataaaaaagc ggcaactcaa taaagttgcc gctttacgag gaaattagaa cattacctta
      181 tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt cggtggttta
      241 acaccatcga gtttgtattc ttcacccatc gccacccatt tgtgtttgcc cagttcgtgg
      301 taggggagaa gttcgatttt ctcaacgtta cccatatcac gggtaaattc accgaggcga
      361 tgtgccgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc
      421 acatttttgt tcgccagata tttagcgaac tccagcgtgc ggtggttgga aactccaacc
      481 agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact
      541 tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt atccagacag
      601 gtatgaatgc cttctttttt acaggcgcgg aaccagtcac gaacaaactc agcttgcagg
      661 attgcttcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag
      721 gtcaccactt ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag
      781 gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag
      841 cgaatacctg ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact
      901 gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa
      961 ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct
     1021 ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga
     1081 tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct
     1141 gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg
     1201 gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt
     1261 tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag
     1321 ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg
     1381 tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg
     1441 ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc
     1501 cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga
     1561 tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct
     1621 tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa
     1681 actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg
     1741 gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga
     1801 taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt
     1861 agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca
     1921 gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagctcagg agatcgcctt
     1981 tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga
     2041 ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac
     2101 caacgatcat cgggcttacg cagcaagcga tagcgtagtc atcgttgttg aagtccggac
     2161 gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga
     2221 atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag
     2281 acggccccat agtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac
     2341 gaccatcgag gcccatacca ccgatagatt cggttgccca gattgggtcg ccagagaaca
     2401 gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt
     2461 caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt
     2521 acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa
     2581 cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac
     2641 cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct
     2701 gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat
     2761 cagcctgtag agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca
     2821 gcgcaacgcg acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg
     2881 tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct
     2941 ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt
     3001 tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt
     3061 tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttattg atgtagccag
     3121 cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa atcaactggc gcgtgagtgc
     3181 ggttttccag tttaacacct tccattactt tgtcccacag ggtggtggtc gcttcagtag
     3241 cgccagccag gaaggactcg tcaccctcgt acggcgtgta gtttttctga atgaagtcac
     3301 ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact
     3361 tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt
     3421 acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc
     3481 caaccaacaa accaccgccg ataatattac cgatcgtaac cgggatcagg ttatcagtga
     3541 tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga
     3601 attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga
     3661 tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt
     3721 tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc atcaggtttg
     3781 ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt
     3841 gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta
     3901 aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttag
     3961 ccaactgaca ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag
     4021 taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc
     4081 caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga
     4141 agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg
     4201 gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa
     4261 gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac
     4321 gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta
     4381 taggcccgta actcgcatgg tttttatgca aatacggagt aactatttga ttatccaaat
     4441 aaaaataaat tttaaaaatt aacaaatgag ttgaattttt tccgcatcct ccgctaaaac
     4501 agttaattaa aagggagcat caggcgaata aagtaacaat atcgatcgta tttattaaat
     4561 acaaattacc gatatttaac cttataatta caattatttt attaatgcaa atatatgtaa
     4621 agcggggcat taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg
     4681 cgtaggctat ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt
     4741 aggccttcaa caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca
     4801 ggccgtaaaa cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc
     4861 cgtacatgcg aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca
     4921 gtaataacaa cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg
     4981 agttaaactc catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca
     5041 gcgccagcat ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg
     5101 tcgccagacc caacagctca cgcacgcggg taaagtcatc aaaatcttct tcatccagtt
     5161 gctcgatgag gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg
     5221 tttcacgtaa atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa
     5281 tatcggacat gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg
     5341 caatataaac ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt
     5401 ccgtggtgcc ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt
     5461 cccaggagat taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga
     5521 cttcttcatc atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt
     5581 gcagcagctc ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag
     5641 aggcaaagca ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc
     5701 cgccaaggga accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg
     5761 cttcaaccac cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc
     5821 tttcagcaat aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc
     5881 gtgcttcgtt gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga
     5941 tattcatcgg aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa
     6001 taccacgatc ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct
     6061 ccggatcgta aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt
     6121 cgctcagtgg gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt
     6181 cgcccagcca gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga
     6241 gtgcagaagc cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc
     6301 actctttgtc gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct
     6361 cttcaatctg aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca
     6421 gagcggcatc tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga
     6481 aagcgcgcaa taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa
     6541 ctgttgacta tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga
     6601 tctccaccgt atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg
     6661 tagtctttat acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     6721 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     6781 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     6841 ttgttatacc gcctggtaat atgatggcga gctggcctgg tcgaaactca ataactccaa
     6901 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     6961 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     7021 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     7081 gccggagtcg gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg
     7141 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     7201 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     7261 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     7321 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     7381 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     7441 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     7501 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     7561 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     7621 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     7681 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     7741 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     7801 ggttaagcca atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc
     7861 gaatgaaacc atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt
     7921 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     7981 tgtaatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     8041 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     8101 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     8161 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     8221 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     8281 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     8341 caaattgttc cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt
     8401 ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct
     8461 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     8521 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     8581 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     8641 tttctaaccg cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc
     8701 tgctggatag cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct
     8761 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     8821 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     8881 cagctctttg tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc
     8941 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     9001 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     9061 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     9121 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     9181 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     9241 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     9301 cttcggcttc ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     9361 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     9421 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     9481 ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat
     9541 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     9601 gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg
     9661 attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     9721 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     9781 ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
     9841 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     9901 cggcacattg t
//
LOCUS       DADLDT020000136         9857 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000136 DADLDT020000000
VERSION     DADLDT020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.137"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            389..2047
                     /locus_tag="JQH22_003479"
     CDS             389..2047
                     /locus_tag="JQH22_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFB2207682.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="JQH22_003480"
     CDS             complement(2044..3000)
                     /locus_tag="JQH22_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFB2207683.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="JQH22_003481"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="JQH22_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFB2207684.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="JQH22_003482"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="JQH22_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFB2207685.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQH22_003483"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQH22_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFB2207686.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="JQH22_003484"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="JQH22_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFB2207687.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="JQH22_003485"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="JQH22_003485"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFB2207688.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2418 a         2294 c         2740 g         2405 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaat
//
LOCUS       DADLDT020000137         9648 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000137 DADLDT020000000
VERSION     DADLDT020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.139"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(59..379)
                     /gene="psiF"
                     /locus_tag="JQH22_003486"
     CDS             complement(59..379)
                     /gene="psiF"
                     /locus_tag="JQH22_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFB2207689.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(498..1913)
                     /gene="phoA"
                     /locus_tag="JQH22_003487"
     CDS             complement(498..1913)
                     /gene="phoA"
                     /locus_tag="JQH22_003487"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFB2207690.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2014..2274)
                     /gene="iraP"
                     /locus_tag="JQH22_003488"
     CDS             complement(2014..2274)
                     /gene="iraP"
                     /locus_tag="JQH22_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFB2207691.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2456..2659)
                     /locus_tag="JQH22_003489"
     CDS             complement(2456..2659)
                     /locus_tag="JQH22_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207692.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2737..3831
                     /gene="ddlA"
                     /locus_tag="JQH22_003490"
     CDS             2737..3831
                     /gene="ddlA"
                     /locus_tag="JQH22_003490"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFB2207693.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3855..4067)
                     /locus_tag="JQH22_003491"
     CDS             complement(3855..4067)
                     /locus_tag="JQH22_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFB2207694.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4327..4635
                     /locus_tag="JQH22_003492"
     CDS             4327..4635
                     /locus_tag="JQH22_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFB2207695.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4694..5788)
                     /gene="yaiW"
                     /locus_tag="JQH22_003493"
     CDS             complement(4694..5788)
                     /gene="yaiW"
                     /locus_tag="JQH22_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFB2207696.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5801..7021)
                     /gene="sbmA"
                     /locus_tag="JQH22_003494"
     CDS             complement(5801..7021)
                     /gene="sbmA"
                     /locus_tag="JQH22_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFB2207697.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7373..8530
                     /gene="ampH"
                     /locus_tag="JQH22_003495"
     CDS             7373..8530
                     /gene="ampH"
                     /locus_tag="JQH22_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFB2207698.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8531..9034)
                     /locus_tag="JQH22_003496"
     CDS             complement(8531..9034)
                     /locus_tag="JQH22_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2207699.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9122..>9648)
                     /locus_tag="JQH22_003497"
     CDS             complement(9122..>9648)
                     /locus_tag="JQH22_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFB2207700.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2398 a         2463 c         2295 g         2492 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca atcaggtatc
       61 aggcggcttt cttgaggcag gcactcataa acttattacg atcatcacct ttcagagctt
      121 gttgcgttgc ttgattatta cattcacgca tcttttgctg ctgtggcgtc aaactttttt
      181 caccaggcgc agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc
      241 ctttcaacgc ctgcgccgtc gcctgctgat tacaggaggt catacgctgt tgttgtgggg
      301 ttaaggttct ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa
      361 ccagtaatgt tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtttgcgc
      421 ccgtgatctg ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt
      481 cactgccggg cgcagtttta tttcagcccc agagcggctt tcatggtgta gaagagatcg
      541 gtctggtcgg tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac
      601 tgactgccgg tatgttcttg tgaatcctct tcggaattcc cgtaactcat caccatcact
      661 gcgccatctt tagtatttag cgcctgagtg aggcccggcg ctttggtatc cggcgcaaca
      721 acctggctgg cgtgggcgtg atcagcggtg actatgacca gcgtgttacc ctccttttta
      781 gcgaattcca gcgcccgttg tacggcttca tcgagatcga ccgtctcgcc aatttgccca
      841 caagggttcg cagcgtgatc ctgtttatcg attgacgcac cttcaacttg caggaaaaat
      901 cctttctcat ttttactcaa caattcaatg gctttgtcgg tcatctgtgc cagggttggt
      961 acactgtcat tacgttgcgg attaggcgta taggtgactg cgggcttatc gatattgccg
     1021 tggtacgttg ctttcggccc tagccagcgc actggcatat tgccgtcagc aaacaggcca
     1081 agcaggggtt tttgctgatt cgcttccgtc accgaattca gtgaggcagc atcgttcacc
     1141 aactgataac cacgcgcctg tgcctgttca cgcagcgttt ttccctgcca ttcaccagcg
     1201 gttgccgttt cagcaaaggt ttttgcgccg ccgccaagcg taacgtcggc acgagcgtta
     1261 agcagctgtt cggtaatcga tccttttccg cctttttcca gagcgttacc cggacatttt
     1321 tcactggtcg cgctcggacc gtagcatttg cgcgaagtca catgtgccac cagcgcagcg
     1381 ggcgtggcat cctgcaactc tgcggtagaa acgttaccgg tcgccagacc tgcggctttt
     1441 gccatttcca gaatcgttgg gtgatctttt tcgtgaatat cgacgcccag cgcgccgtta
     1501 taggttttga caccggttga ccaggcggtt gctgatgcag ccgagtcggt gacgtagtcc
     1561 ggtttgccag tttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca
     1621 tcgatacctt taaaaaagcc gcccgcacct tcggcataat tacgtgcggc agtaatttcc
     1681 gaatccccca tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga
     1741 gaatcacgca gagcggcggt ctgatcaccc gttaagcggc gagcaccgcc gggtgcagta
     1801 atatcgccct gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc
     1861 acaggggtaa acagtaacgg taagagtgcc agtgcaatag tgctttgttt cactttattt
     1921 tctccatgta caaatacatt aaaaaataaa aacaaagcga ctataagtct cggccgtgac
     1981 aactttatga cagctgttga aaagattaac tttttactga caaggatgct tcaataactt
     2041 ctttacgtaa tcgcgcagca gctctgtatc gtcgtcagga atgctggcat cgggctttac
     2101 ctcgtacagc gccccctcta cctgatcaat caaccgctgt tggtcatttt gcgccatatt
     2161 gcgaagcatt gcagtgacga taatctccaa agcttctacc tgcgcacaca gttctttcga
     2221 ctcttcttct ttttgggcaa gcttaaataa caactcagca atgagatttt tcatgtctgt
     2281 atttccttat ccaaagtatg gagaagttaa cattatgttt cacaacttca tagtgtctgg
     2341 aagtattatt tttatgacaa aggaaatact ttgcgcaaat attttttgtt tgattaccag
     2401 cgaaacgttt ctcttattta aataattacc cacacaaaat ataggctttt aattatcaac
     2461 ctttattata aataaaagct atgaaatata ttctcttcgt taacttataa ttaagagaaa
     2521 aaaactcacc gcattgccat ttaagccagg aataaattag cttcccgttc atcatttttg
     2581 tgatcttgcg cacatctttg agataacgca ctttcttcac cagtacgttg cacgctttgt
     2641 cagcagaaga ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat
     2701 gtatgcactt tgtgttagac gggataggtt tttaagatgg aaaaactgcg ggtaggaatc
     2761 gtttttggtg gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc
     2821 gatgccattg ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa acaagggcaa
     2881 tggcacgtca gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg
     2941 ttgcgccctt cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc
     3001 gacgcgcaaa acggtcagcc gttgccgacg gtggatgtca ttttcccgat tgtccacggt
     3061 acgctgggcg aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta
     3121 ggttctgatg ttctggcttc agcagcctgt atggataaag atgtcaccaa acgtctgcta
     3181 cgcgatgccg ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac
     3241 atcagttttg ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa accggctaat
     3301 cagggctctt ctgttggtgt cagcaaagta accagtgaag aacagtacgc aattgccgtc
     3361 gatctggcgt tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgag
     3421 atcgaatgcg cagttctggg caacgacaat ccgcaagcca gcacctgtgg cgagatcgta
     3481 ctcaccagcg atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg
     3541 gtagttccgg cagccattgc accagagatc aacgataaga tccgggcgat tgccgttcag
     3601 gcttatcaga cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag
     3661 aacgaagtgg tgatcaacga gatcaacaca ctgcctggct tcaccaacat cagtatgtat
     3721 ccgaagctgt ggcaagccag cggtctgggt tacaccgatc tgatcacacg tttgattgag
     3781 ctggcgctgg agcgtcacgc tgcggataac gcattgaaaa ccacaatgta atattttaac
     3841 gcccgataat attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcaac
     3901 cccgccagcc agaaactaat cacccacgtc accagaccga cggcataggt ctgccaacct
     3961 tttgcttcaa atcccagtac ccccaccacg ccattaagaa tgaatataag gccaatggcg
     4021 aacgcatagt agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta
     4081 acgaaaaaca acatccttgc atattctgta acaaccgtct gtggtgtaga caacattttc
     4141 gtaattatta ataaatttaa cttaaggttg ccattttgtg actcacaacg aagattcacg
     4201 cttcgggaat attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc
     4261 tgtcagactt gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag
     4321 gtttttatgg ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc
     4381 tcttcaacgc ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg
     4441 gcgcaattgc tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc
     4501 caggaaacag gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg
     4561 atcccgtttt tagcaggctg cctcattttt gcagtggtgg cgctatggct gcactggcga
     4621 catcgccgcc agtaaaattt acgttgccag atgcggcctg aaacgcctta tctggcctga
     4681 cattcctgtt tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa
     4741 accaggccgt agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca
     4801 tttcgcgggg tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca
     4861 gcgccgtctc ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt
     4921 cgctcatccc cagttttccc gccagtttgc gcgttgccag ttcggttttc ctgggttctt
     4981 tactgtcgta acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga
     5041 ctgcgttctg aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc
     5101 ggtatatcgg tgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca
     5161 acccgccgcg gcgactgaag acttcctgac gaactgtacc gtccattttc cacggatacc
     5221 ctttggtatg ctgttcggca aaagcaatgc tgacctgcat cggcccaccg gtgcgcaccg
     5281 gattaagcga accaaacagc gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa
     5341 tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg ttcgctataa cttttgccgt
     5401 ttggcgattt gatttttagc gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag
     5461 cacgacggtc aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct
     5521 gataatttga ctcctgctgc gccaccgcca gcaccgagta gacgttttcc agcgtcggtg
     5581 ccagcccctg actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca
     5641 cgctcgcagg catcttctgc cgcacgacgc tcgctacatc tatcgctttt tcgccctttt
     5701 tcaacggttg cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca
     5761 gcgaagatag atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta
     5821 cttcctgaat tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag
     5881 acatcaactc aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct
     5941 gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg
     6001 acggaaacag caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga
     6061 tgttgaaata catatagtga aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca
     6121 gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt
     6181 tacggtaggc ggcctctact cgctggtttt taaactccag ccccggcagt ttgatcccta
     6241 ccactgccag caatccggtc cccatcagcg accagacgat agcggcaatc accagaccat
     6301 acggaatgtg cccgataatc ggcagctccg gcacatgcgc ggagagcgtt accagcaccg
     6361 gcaggaaggc gatcaacgtc attatggcgt tgataaaact gacgcccata ttctccagcg
     6421 ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca
     6481 gttgttgcca gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacgtagt
     6541 gactgacaaa gaagttgttc agtacactga tcaccactgc gatcagcgca atccccagaa
     6601 agacgcccac ttcgcggtaa aattgttcga tagtgacttt atgcggcgaa cttagcgcgg
     6661 tttgaatcag atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca
     6721 aaaaccaagt gacgaagata atcagcgcag taccgagtat tgaccagtat tgccaacggt
     6781 gcgggctgta gataaaccag aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt
     6841 aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag
     6901 cgccggtgat atgtgccacc cagtcacccc caccggcttg ccagaagata acggcaatca
     6961 atgcccaaac aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca
     7021 tatgcactcc tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg
     7081 ctacaaagcg tcagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg
     7141 ccagcctgac cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag
     7201 cggattaaca cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt
     7261 tacgcgccgt tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa
     7321 tgagtagtat aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg
     7381 tagtctgctt ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca
     7441 gccgatcact gaaccggagt ttgcctctga tattgtcgat cgttatgccg atcatatttt
     7501 ttacggcagc ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt
     7561 tcgtagttat ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg attcggtcgt
     7621 gcgtattgct tccctcacca aactaatgac cagtgaaatg ctggtgaaat tgctcgacca
     7681 ggggaccgta aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtgcc
     7741 gacctacaat gggacgccca ttaccctagt gaatctggca acccatacca gcgccctgcc
     7801 ccgtgaacag cccggtggcg cggcacatcg tccggtgttt gtctggccaa cgcgcgagca
     7861 acgctggaaa tacctttcta cggcgaagct gaaagccgcg ccaggcagtc aggcggcgta
     7921 ctctaacctt gcgttcgacc tgctggcaga tgcactggcg aatgcgtccg gcaagcctta
     7981 tacccagttg tttgaagagc agattaccca tccattgggg atgaaagaca ccacctacac
     8041 cccctcaccg gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa
     8101 taacacgctg gcggcaattg gcagcggcgg cgtctattcc acgcctggcg atatgatgcg
     8161 ctggatgcag cagtatttgt catctgattt ttatcagcgc agtaaccagg cagatcgcat
     8221 gcagacgttg atttatcagc gcgcgcagtt tacgaaagtg attggcatgg atgtccccgg
     8281 caaagcggat gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccgag
     8341 gattattcag aagacaggcg gtggtggcgg tttcattacc tatatggcga tgatcccgca
     8401 gaagaatatt ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat
     8461 gagcgatggc atcaatgatc tggtgaccga gctaagcggg aataaaccgt tggttatccc
     8521 cgcatcctga ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca
     8581 tttcgatata gccgccttta cgcaaagcag cgagaacttc tgcgacgaca gaacgcgata
     8641 tgcgtgtacg ttgatggata taattcatca caccaatacg tgatcgcaat tcttcattcc
     8701 agtcaatcat tgataataat gttgcgcggg tttgtttggc tggtagatga tatcccgtac
     8761 aatttccttc tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagcc
     8821 ccataatata aggagcctgg gtaataccga taagtacgtt ttcttctcta cgcagagaga
     8881 taactccttc taaaataacg aaagtatctt cgttattgac atcactggaa aatataactt
     8941 gcttttcatt attaaactcg aagcgcgtac cgtatctgga caaacattta tcgagcttac
     9001 caaattcctg aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg
     9061 tcaaatcctt gacgggcatt atttagatta aattaccagt atttcttcgg agtgaagaat
     9121 attaccaagt atatttaaca cccacgttcg cggaccagtc ttgatctacg tcaccaccac
     9181 cgaggtagtt agcatcggta taggcgctga agttcttggt gaagctgaac tgagtaccca
     9241 gaccaacacg taccgcagac ccttcagtac cgttatcgat ggaatcaccg ttcacatcgt
     9301 tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc agagcctgat
     9361 cttcgctgta ggtgaaggta taacctgcat ctacacccag ttcataacgc atgctgtcgt
     9421 aaggctgacc gtcaactttc atgtcgttgc tcagctggta gtcatcacca gactggaaca
     9481 gaccagaaat gctgccgtaa ggagtcacgt aaccagcatc acccagtttg aagtcgtaac
     9541 cggttttcaa accgaagccc caagcgtcgg agttggtgct accgtcaacg taagtaccgt
     9601 tgctcatggt tgcagacagg tcgttgttga agtgagagta gctcaagc
//
LOCUS       DADLDT020000138         9640 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000138 DADLDT020000000
VERSION     DADLDT020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.140"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="JQH22_003498"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="JQH22_003498"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFB2207701.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="JQH22_003499"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="JQH22_003499"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFB2207702.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="JQH22_003500"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="JQH22_003500"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFB2207703.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="JQH22_003501"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="JQH22_003501"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFB2207704.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="JQH22_003502"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="JQH22_003502"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFB2207705.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5059..6192
                     /locus_tag="JQH22_003503"
     CDS             5059..6192
                     /locus_tag="JQH22_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFB2207706.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="JQH22_003504"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="JQH22_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFB2207707.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="JQH22_003505"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="JQH22_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFB2207708.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="JQH22_003506"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="JQH22_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFB2207709.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="JQH22_003507"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="JQH22_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFB2207710.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="JQH22_003508"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="JQH22_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFB2207711.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="JQH22_003509"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="JQH22_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFB2207712.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2472 c         2459 g         2258 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accaactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg
     2221 cacgttggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatcc ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctagttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggtgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tactccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 cccccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg
     6361 cgtgaccgta gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
     6481 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacggtaata acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 aaatttgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc
     7261 agaaaaaaca aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac
     9421 gcttatcagg cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg
     9481 ataaggcgtt tacgccgcat ccggcaagag caacaaacgc aagtacagca acctgtcatt
     9541 aaactgagaa atttaatctt tgcacgttgc ataaccttta attattcata tgcattgctg
     9601 ttgttacttt ttatatagct aacaccaata attaaccttt
//
LOCUS       DADLDT020000139         9625 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000139 DADLDT020000000
VERSION     DADLDT020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.141"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JQH22_003510"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JQH22_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFB2207713.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JQH22_003511"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JQH22_003511"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFB2207714.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="JQH22_003512"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="JQH22_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="JQH22_003513"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="JQH22_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFB2207715.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JQH22_003514"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JQH22_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFB2207716.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="JQH22_003515"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="JQH22_003515"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFB2207717.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="JQH22_003516"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="JQH22_003516"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFB2207718.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="JQH22_003517"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="JQH22_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="JQH22_003518"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="JQH22_003518"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFB2207719.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2353 a         2515 c         2488 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtagga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DADLDT020000140         9493 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000140 DADLDT020000000
VERSION     DADLDT020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.142"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..190)
                     /locus_tag="JQH22_003519"
     CDS             complement(<1..190)
                     /locus_tag="JQH22_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFB2207720.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="JQH22_003520"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="JQH22_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFB2207721.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="JQH22_003521"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="JQH22_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFB2207722.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="JQH22_003522"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="JQH22_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFB2207723.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="JQH22_003523"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="JQH22_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="JQH22_003524"
     CDS             complement(3262..3381)
                     /locus_tag="JQH22_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFB2207724.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="JQH22_003525"
     CDS             complement(3342..3527)
                     /locus_tag="JQH22_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207725.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="JQH22_003526"
     CDS             3628..3801
                     /locus_tag="JQH22_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207726.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="JQH22_003527"
     CDS             3803..4147
                     /locus_tag="JQH22_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFB2207727.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="JQH22_003528"
     CDS             4151..4486
                     /locus_tag="JQH22_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFB2207728.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="JQH22_003529"
     CDS             complement(4543..6864)
                     /locus_tag="JQH22_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFB2207729.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="JQH22_003530"
     CDS             complement(6981..7190)
                     /locus_tag="JQH22_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207730.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="JQH22_003531"
     CDS             complement(7941..9464)
                     /locus_tag="JQH22_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFB2207731.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DADLDT020000141         9358 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000141 DADLDT020000000
VERSION     DADLDT020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.143"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="JQH22_003532"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="JQH22_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="JQH22_003533"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="JQH22_003533"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFB2207732.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="JQH22_003534"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="JQH22_003534"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFB2207733.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="JQH22_003535"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="JQH22_003535"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFB2207734.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="JQH22_003536"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="JQH22_003536"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFB2207735.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="JQH22_003537"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="JQH22_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFB2207736.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="JQH22_003538"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="JQH22_003538"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFB2207737.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="JQH22_003539"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="JQH22_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFB2207738.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="JQH22_003540"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="JQH22_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFB2207739.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="JQH22_003541"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="JQH22_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFB2207740.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..>9358)
                     /locus_tag="JQH22_003542"
     CDS             complement(9203..>9358)
                     /locus_tag="JQH22_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207741.1"
                     /translation="AIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQ
                     QKVVNED"
BASE COUNT         2399 a         2341 c         2376 g         2242 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagc
//
LOCUS       DADLDT020000142         9254 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000142 DADLDT020000000
VERSION     DADLDT020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.144"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JQH22_003543"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JQH22_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFB2207742.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLA
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JQH22_003544"
     CDS             1828..3147
                     /locus_tag="JQH22_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HFB2207743.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLHDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="JQH22_003545"
     CDS             3445..3486
                     /locus_tag="JQH22_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207744.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="JQH22_003546"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="JQH22_003546"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFB2207745.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JQH22_003547"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JQH22_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="JQH22_003548"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="JQH22_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="JQH22_003549"
     CDS             5815..6711
                     /locus_tag="JQH22_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFB2207746.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="JQH22_003550"
     CDS             6708..7694
                     /locus_tag="JQH22_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFB2207747.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="JQH22_003551"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="JQH22_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="JQH22_003552"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="JQH22_003552"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFB2207748.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTTPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2262 a         2262 c         2320 g         2410 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggc tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaca tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg gtggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caag
//
LOCUS       DADLDT020000143         9088 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000143 DADLDT020000000
VERSION     DADLDT020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.146"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQH22_003553"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQH22_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFB2207749.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQH22_003554"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQH22_003554"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFB2207750.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQH22_003555"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQH22_003555"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFB2207751.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQH22_003556"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQH22_003556"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFB2207752.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="JQH22_003557"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="JQH22_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFB2207753.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="JQH22_003558"
     CDS             5545..6018
                     /locus_tag="JQH22_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HFB2207754.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="JQH22_003559"
     CDS             6047..6589
                     /locus_tag="JQH22_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFB2207755.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="JQH22_003560"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="JQH22_003560"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFB2207756.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="JQH22_003561"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="JQH22_003561"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFB2207757.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="JQH22_003562"
     CDS             7986..8243
                     /locus_tag="JQH22_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFB2207758.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="JQH22_003563"
     CDS             complement(8219..8773)
                     /locus_tag="JQH22_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFB2207759.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2282 a         2203 c         2266 g         2337 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 tgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaagaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
     9061 tacgccacct cgcgacagtg cgctaaag
//
LOCUS       DADLDT020000144         9080 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000144 DADLDT020000000
VERSION     DADLDT020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.147"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            18..491
                     /locus_tag="JQH22_003564"
     CDS             18..491
                     /locus_tag="JQH22_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFB2207760.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            622..1410
                     /locus_tag="JQH22_003565"
     CDS             622..1410
                     /locus_tag="JQH22_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFB2207761.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1616..1963
                     /locus_tag="JQH22_003566"
     CDS             1616..1963
                     /locus_tag="JQH22_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFB2207762.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1957..2796
                     /gene="sul1"
                     /locus_tag="JQH22_003567"
     CDS             1957..2796
                     /gene="sul1"
                     /locus_tag="JQH22_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFB2207763.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2924..3127
                     /locus_tag="JQH22_003568"
     CDS             2924..3127
                     /locus_tag="JQH22_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207764.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            3283..4488
                     /gene="chrA"
                     /locus_tag="JQH22_003569"
     CDS             3283..4488
                     /gene="chrA"
                     /locus_tag="JQH22_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFB2207765.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            4499..4804
                     /locus_tag="JQH22_003570"
     CDS             4499..4804
                     /locus_tag="JQH22_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2207766.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            4848..4913
                     /locus_tag="JQH22_003571"
                     /pseudo
     CDS             4848..4913
                     /locus_tag="JQH22_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5031..5795
                     /locus_tag="JQH22_003572"
     CDS             5031..5795
                     /locus_tag="JQH22_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFB2207767.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(6288..6872)
                     /locus_tag="JQH22_003573"
     CDS             complement(6288..6872)
                     /locus_tag="JQH22_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFB2207768.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(6872..8110)
                     /locus_tag="JQH22_003574"
     CDS             complement(6872..8110)
                     /locus_tag="JQH22_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207769.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8107..9012)
                     /locus_tag="JQH22_003575"
     CDS             complement(8107..9012)
                     /locus_tag="JQH22_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFB2207770.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1874 a         2607 c         2684 g         1915 t
ORIGIN      
        1 ccattaaggg agttaaattg aaaatatcat tgatttctgc agtgtcagaa aatggcgtaa
       61 tcggtagtgg tcctgatatc ccgtggtcag taaaaggtga gcaactactc tttaaagcgc
      121 tcacatataa tcaatggctc cttgtcggaa gaaaaacatt tgactctatg ggtgttcttc
      181 caaatcgcaa atatgcagta gtgtcaaaga acggaatttc aagctcaaat gaaaacgtcc
      241 tagtttttcc ttcaatagaa aatgctttga aagagctatc aaaagttaca gatcatgtat
      301 atgtctctgg cgggggtcaa atctataata gccttattga aaaagcagat ataattcatt
      361 tgtctactgt tcacgttgaa gtcgaaggtg atatcaaatt ccctataatg cctgagaatt
      421 tcaatttggt ttttgaacag ttttttatgt ctaatataaa ttatacatac cagatttgga
      481 aaaaaggcta acaatgcgtt gcagcaccag tcgcttcgct ccttggacag cttttaagtc
      541 gcgtctttgt ggttttgctg cgcaaaagta ttccacaaag ccgcaactta aaagctgccg
      601 ctgaacttaa cgttaggcat catgggtgaa tttttccctg cacaagtttt caagcagctg
      661 tcccacgctc gcgcggtgat cgagcgccat ctggctgcga cactggacac aatccacctg
      721 ttcggatctg cgatcgatgg agggctgaag ccggacagcg acatagactt gctcgtgacc
      781 gtcagcgccg cacctaacga ttcgctccgg caggcgctaa tgctcgattt gctgaaagtc
      841 tcatcaccgc caggcgatgg cggaacatgg cgaccgctgg agctaactgt tgtcgctcga
      901 agcgaagtag tgccttggcg ctatccggcg cggcgtgagc ttcagttcgg tgagtggctc
      961 cgccacgaca tcctttccgg aacgttcgag cctgccgttc tggatcacga tcttgcgatt
     1021 ttgctgacca aggcgaggca acacagcctt gcgcttctag gcccatccgc agccacgttt
     1081 ttcgagccgg tgccgaagga gcatttctcc aaggcgcttt tcgacactat tgcccagtgg
     1141 aatgcagagt cggattggaa gggtgacgag cggaacgtcg ttcttgctct tgctcgcatt
     1201 tggtacagcg cttcaactgg tctcattgct cctaaggacg ttgctgccgc atgggtatcg
     1261 gagcgtttgc ctgccgagca tcggcccctc atctgcaagg cacgcgcggc gtacctgggt
     1321 agcgaggacg acgacctagc aatgcgcgtc gaagagacgg ccgcgttcgt tcgatatgcc
     1381 aaagcaacga ttgagagaat cttgcgttga gcggcatgtg cgaaaagtgc atcgacccgc
     1441 gccgagggca tctgatgcct aactcggcgt tcaagcggac gggctgcgcc cgccgctcaa
     1501 ctatgcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     1561 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa
     1621 aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa
     1681 atctagcgag ggctttacta agcttgcccc ttccgccgtt gtcataatcg gttatggcat
     1741 cgcattttat tttctttctc tggttctgaa atccatccct gtcggtgttg cttatgcagt
     1801 ctggtcggga ctcggcgtcg tcataattac agccattgcc tggttgcttc atgggcaaaa
     1861 gcttgatgcg tggggctttg taggtatggg gctcataatt gctgcctttt tgctcgcccg
     1921 atccccatcg tggaagtcgc tgcggaggcc gacgccatgg tgacggtgtt cggcattctg
     1981 aatctcaccg aggactcctt cttcgatgag agccggcggc tagaccccgc cggcgctgtc
     2041 accgcggcga tcgaaatgct gcgagtcgga tcagacgtcg tggatgtcgg accggccgcc
     2101 agccatccgg acgcgaggcc tgtatcgccg gccgatgaga tcagacgtat tgcgccgctc
     2161 ttagacgccc tgtccgatca gatgcaccgt gtttcaatcg acagcttcca accggaaacc
     2221 cagcgctatg cgctcaagcg cggcgtgggc tacctgaacg atatccaagg atttcctgac
     2281 cctgcgctct atcccgatat tgctgaggcg gactgcaggc tggtggttat gcactcagcg
     2341 cagcgggatg gcatcgccac ccgcaccggt caccttcgac ccgaagacgc gctcgacgag
     2401 attgtgcggt tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt cgctgccgac
     2461 cggctcatcc tcgatccggg gatgggattt ttcttgagcc ccgcaccgga aacatcgctg
     2521 cacgtgctgt cgaaccttca aaagctgaag tcggcgttgg ggcttccgct attggtctcg
     2581 gtgtcgcgga aatccttctt gggcgccacc gttggccttc ctgtaaagga tctgggtcca
     2641 gcgagccttg cggcggaact tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac
     2701 gcgcctggag atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt
     2761 cgcgacgcca gagaccgagg gttagatcat gcctagcatt caccttccgg ccgcccgcta
     2821 gcggaccctg gtcaggttcc gcgaaggtgg gcgcagacat gctgggctcg tcaggatcaa
     2881 actgcactat gaggcggcgg ttcataccgc gccaggggag cgaatggaca gcgaggagcc
     2941 tccgaacgtt cgggtcgcct gctcgggtga tatcgacgag gttgtgcggc tgatgcacga
     3001 cgctgcggcg tggatgtccg ccaagggaac gcccgcctgg gacgtcgcgc ggatcgaccg
     3061 gacattcgcg gagaccttcg tcctgagatc cgagctccta gggatcgcct cagaaaacgg
     3121 aaaataaagc acgctaagcc gtaagtaagc gtgctcctgt gaaagccaca gctaaaactg
     3181 cgtagtacac atagaggtat ttttttctag atttatatcg aatttcgtta tcgaatttcg
     3241 cttattattg atcaactcgt tgcagatcat gaaggcgaaa gtatgacgaa taaccctacc
     3301 gatgacagca gaccatggtc ggtctttctt atttttctgc ggcttggatt gacatctttt
     3361 ggcggcccca ttgcgcactt gggctacttc cgcgccgaat ttgtcacacg gcggcgctgg
     3421 ctctccgaac ggagctatgc tgacttggtc gcgctttgtc agttcttgcc agggcctgca
     3481 agcagccagg tcggcatagc ggtaggactg tctcgggctg gatacagcgg ggcgctggct
     3541 gcttgggctg gcttcacgct gccgtctgcc atagccttga tcctttttgc gctcggcatc
     3601 tccagctatg gcgattacgt ctcgcagggc gcgttgcatg gcttaaaagt ggtggctgtg
     3661 gccgtggtcg ctcaagcagt atggggcatg gcgcgtaacc tatgcacgga tgggctgcga
     3721 gtcaccatca tggcaattgc tacctgcgtc gttttacttg tgccgtccgc gtggggacag
     3781 gttggcgtga ttgctatcgc aggcatcgca ggccggttat tgttcaagcc agcgaaagtt
     3841 gttgagcatg accccctacc tatcacggtc agtcaccggg ccggcgtgct ttggctctcg
     3901 ctgttctttg tcttgctgat tggcctgccg gtgttggccg aactgatgcc aagtcaaacc
     3961 atggcaatgg tggattcctt ctatcgtgtc ggatcactgg tgttcggcgg tggtcacgtt
     4021 gtgctgccat tactgcaagc cgaagtggtg ccctccggct gggtcaacaa tgaatccttt
     4081 ctcgcggggt acggggcagc tcaagcggtg cccggccctt tgttcacgtt cgccgcgttt
     4141 cttggtgcct cgatgaacac cgccccgtcg ggctggatcg gcggcattgt gtgtctgctg
     4201 gctatcttcg cgccctcgtt cttgctggtc gtcggatcaa tgccattttg ggagcgtttg
     4261 cgccgcaata caggcatcca agctgcgctg gccgggatca atgccgctgt agtcggcttg
     4321 ctgctggccg cgctgtatca gcctgtatgg actagcgcca tctttcagcc gcaagacttc
     4381 ggcttggcat tagttgccct tgtcgcactt atgttctgga agctcccgcc gtggctggtt
     4441 gtcctcggta gcggtgcagc tgggtggctg ttgagcgtcg ctctgtgagg cacaaaaaat
     4501 gactgacaaa gacctctacg gcggtttgat ccgcctgcac atccttcacc atgcagccga
     4561 ggaacctgtc tttgggctgg ggatcatcga agagctacgc cgacacggct acgagatgag
     4621 cgctggcacc gtgtacccga tgctgcacgg cctggaaaaa aaaggctatc tgacctcacg
     4681 ccacgaacgc accgggcgac gtgaacggcg tgtttatgac atcactgaac aaggcagaac
     4741 tgcacttgct gatgcgaaaa caaaggtcaa ggagctgttc ggagagttgg tagaaggtgg
     4801 ttgagttttt gcttctatgt caggttgagc tataccctaa ctggattgtc attttcagaa
     4861 gacgactgca ccagttgatt gggcgtaatg gctgttgtgc agccagctcc tgacagttca
     4921 atatcagaag tgatctgcac caatctcgac tatgctcaat actcgtgtgg gctctgttgc
     4981 aaaaatcgtg aagcttgagc atgcttggcg gagattggac ggacggaacg atgacggatt
     5041 tcaagtggcg ccatttccag ggtgatgtga tcctgtgggc ggtgcgctgg tattgtcgct
     5101 atccgatcag ctatcgcgac cttgaggaaa tgctggcgga acgcggcatt tcggtcgacc
     5161 atacgacgat ctatcgctgg gtccagtgct acgccccgga gatggagaag cggctgcgct
     5221 ggttctggcg gcgtggcttt gatccgagct ggcgcctgga tgaaacctac gtcaaggtgc
     5281 ggggcaagtg gacctacctg taccgggcag tcgacaagcg gggcgacacg atcgatttct
     5341 acctgtcgcc gacccgcagc gccaaggcag cgaagcggtt cctgggcaag gccctgcgag
     5401 gcctgaagca ctgggaaaag cctgccacgc tcaataccga caaagcgccg agctatggtg
     5461 cagcgatcac cgaattgaag cgcgaaggaa agctggaccg ggagacggcc caccggcagg
     5521 tgaagtatct caataacgtg atcgaggccg atcacggaaa gctcaagata ctgatcaagc
     5581 cggtgcgcgg tttcaaatcg atccccacgg cctatgccac gatcaaggga ttcgaagtca
     5641 tgcgagccct gcgcaaagga caggctcgcc cctggtgcct gcagcccggc atcaggggcg
     5701 aggtgcgcct tgtggagaga gcttttggca ttgggccctc ggcgctgacg gaggccatgg
     5761 gcatgctcaa ccaccatttc gcagcagccg cctgatcggc gcagagcgac agcctacctc
     5821 tgactgccgc caatctttgc aacagagccc gccgtgctag tctgctcggt gatggtggag
     5881 tgaagccaac ccgcaatcgg gttatgaatc tgcatcgcga ttcgcaatca gctgtctctt
     5941 gagcatgtcg aactcctgcg atgttatctc gccgtgctcg tgctgctaca ccaacttgtc
     6001 caactcatcc gccacaccgc tccccaccac ctgcgatggc caggttgttt gctgggcgca
     6061 ttggcggagt atcgtctcga cccgggacac ccacaccggc acaaccttac gggagtgatt
     6121 cactgtcaaa gaatcggccc ggtgctctga cgcaagtatc gggatggtca ccatttgtaa
     6181 gccgtagacc tgagtggtga tcaagacttc gataccaccg accgtaccgg tactaatcga
     6241 cgacggtcgt gttcgtcgcc tgccgcaggg actctgcaca cctccgttta cgcatgtgcc
     6301 tggaggagtt ggaaatcgtc gtgttcggga aacattaaac acaggatggc agcgatctga
     6361 gccagcacat gatcagctag ctcaccatcc ggatcgacgg cccactgcat cgtcgcgcca
     6421 gcgatgaccg agtgcaggag caactcagct gccgcaggag cacctggggg cagtcgcttg
     6481 cggatcccct ccaccaccgc gcggttccgc tggatcgcaa gcgtgcgtag ctccggcacc
     6541 tggagctcgt accaggagat gagatagttc accgagaagt cgttgcgagt gttcatgctc
     6601 cgaacgagca cctgcaaaaa ttcccagagc ccttgcggcc ctgcgcctat cggtatcgca
     6661 ttcaggtaat gccgcacctg ctcgacgccg cgctccatca tcctcaccag cagcgtatcg
     6721 cggttggtga agcgctggat taacgctgcg cgggagagcc ccacctcctt tgctactccg
     6781 ctgagcgtga actctatggg accgcaacgc ttcagcacta cggtggcggc ctcgagtacc
     6841 tcgtcatcgg acttgagctt ggggcggggc atcagtgttc accttctgta tgggttgggg
     6901 cggaggctgt ggctgccgcc gccattgtag caaattgaag acggagcgag agtagagcca
     6961 cgagccccgc aaacacggcc gatacaacga ggccagggag cggaccagca aggtcgacaa
     7021 acgcgccggc cgcaagcata accatgggcg aggctgacag catcaccgcc gagaccgtgc
     7081 cgagtacccg gccgagaagt tctggcggcg tgcggttgta gatggcagcg ttgagaatgg
     7141 gagagactga gccggtcagc agtcccacga gcgcgcccaa caacatcagc accggcacgc
     7201 ctggcaactg tgaaagcaga agcgagccca ccgcagagcc acaaaatgcc accgccagcc
     7261 agttctgcgc tgatatccgg gcgccgaccg acgcatgaat ggcaatgcca aggagaccac
     7321 cagcccccat cattgaggag aacagcccga gctctgctac ttggcgtcct gcatctacaa
     7381 acagcgcagg catgatgacg ctgccgttgg cgccaacgat gcccacgaag atcatcacta
     7441 taccaaagag agggcgcagc aggggttcgc tccagagaaa agcgacgccg gcgcgcatgg
     7501 agagagtcgc cgtcgtggtc atcgtccgag cggcacgcgc gggaagcacc cacgcgccga
     7561 gcagacctgc aaggacggag cagaacgccg tcagcccgag cgttggcgca gcgccaagca
     7621 ggccgattgc ggccccccca agggccgggc cacctagaat cgcgacgttc ccgatcaccg
     7681 ctttcagtga cgagacgcgc tcaacggaga gcccggcgac gtggccgagt ttgggcagct
     7741 cactgtcctg cgcggccata ccgggtgcgt cgaacgcggc accgagcacc acgcaagcga
     7801 tcagcccagt gttcgagagg gcgccaacgg catcgagcag tgggatgctc gccatggcca
     7861 cgccgcccac cacacccgag atcaatgcga cgggcgcgcg cccgaaccga tcgacgaggc
     7921 caccaccaac ccacgcgccg atgatggtcg cgatgacgct gctagcggcc gtggcgcccg
     7981 cccaggccgc gctctttgta tgagacagga cgaaccatgg aagcgcgagg gccgccaccg
     8041 cgttgccgat ccggaagaga aaggtcgccg cgaacagcgt cgcgagcggg ctatatcgac
     8101 gttcgctcat tccgctgcgg cgagctgcgc cttcgccgca gcgaggtact cttcgttacc
     8161 cgagtcgagg gcgaagagtg cgtaggtgac cgccccgaac gcaaggcgct ccgcgatgtg
     8221 gtgggcgagc cgcggccaca cccggccacc ggccgcttca tacgtgagga ggagcttcgc
     8281 gagcccctct tcaccaaaga ccataaggtg cgcggccatg tcgatggcag ggtcatcaac
     8341 gcgggcctcg ctccagtcga tcatcccgct gacgcgctcc gtgttgtcga tgagcacatg
     8401 gcccacgtag agatcgccat gcaccaccac ggagaaatct ggccacgacg aatcgtcgtc
     8461 gagccagcgc tgccaccggt ggaggcgctt gtcgttcacc acgaactcgc gtcggacgcg
     8521 gtcaacgtcg tcggccacct tctgacgggc ctgcgtcggt gtacggatga gcatccccgc
     8581 atccacggcg gcggaaatgg ggacggcatg cagggcggcg agcgcggtcg cgaagctctc
     8641 cgcgaagacc tccgagtcct gcggcacgac ccagtcgggc gtggacgaac caggctggat
     8701 gaccatcgca gtcgagtctt cgagcatggg ataggcaacg agctcggcgt tggccacgcg
     8761 ccagtccggc accgcgaacg gcaggcgatt cttgagcatt gccagcaccc gcgcctctgg
     8821 ttcgaccttc gcgcttacct cggctcggcg cgggatgcgc agcacccacc gacgtccatc
     8881 gtcgacggtg gcgatcacga tcctatagtc gagcccaagc tcattgacag tcagcgggcc
     8941 atggagcttg agcccatgtc gggctgcaag tgcgtacagt tgggaggtat cggcggtcgt
     9001 gactacggtc atgattcact cctgagggct tgacgggttt agccacctaa atgtaacagt
     9061 cacgtcggtt atattcaatc
//
LOCUS       DADLDT020000145         9013 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000145 DADLDT020000000
VERSION     DADLDT020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.148"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="JQH22_003576"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="JQH22_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="JQH22_003577"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="JQH22_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="JQH22_003578"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="JQH22_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="JQH22_003579"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="JQH22_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="JQH22_003580"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="JQH22_003580"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFB2207771.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="JQH22_003581"
     CDS             8509..8952
                     /locus_tag="JQH22_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFB2207772.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2359 a         2178 c         2173 g         2303 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacgc gccagcgtcg
     9001 cacccggcat ttt
//
LOCUS       DADLDT020000146         8990 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000146 DADLDT020000000
VERSION     DADLDT020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.149"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..2887
                     /locus_tag="JQH22_003582"
                     /pseudo
     CDS             1..2887
                     /locus_tag="JQH22_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JQH22_003583"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JQH22_003583"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFB2207773.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="JQH22_003584"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="JQH22_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="JQH22_003585"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="JQH22_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="JQH22_003586"
     CDS             complement(7280..8041)
                     /locus_tag="JQH22_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HFB2207774.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="JQH22_003587"
     CDS             complement(8038..8697)
                     /locus_tag="JQH22_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFB2207775.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2190 a         2387 c         2342 g         2071 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtttcc cccgttactt tattcgtacc ccttataatg gggtgttagc
     8941 cagccagacc cggcatgatt actgccccca gtcgtccatg atccgggggg
//
LOCUS       DADLDT020000147         8970 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000147 DADLDT020000000
VERSION     DADLDT020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.150"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..923
                     /locus_tag="JQH22_003588"
     CDS             <1..923
                     /locus_tag="JQH22_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2207776.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JQH22_003589"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JQH22_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFB2207777.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQH22_003590"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQH22_003590"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFB2207778.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JQH22_003591"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JQH22_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFB2207779.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JQH22_003592"
     CDS             4446..4805
                     /locus_tag="JQH22_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFB2207780.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="JQH22_003593"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="JQH22_003593"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFB2207781.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="JQH22_003594"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="JQH22_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFB2207782.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="JQH22_003595"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="JQH22_003595"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFB2207783.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2038 a         2169 c         2427 g         2336 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc
//
LOCUS       DADLDT020000148         8652 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000148 DADLDT020000000
VERSION     DADLDT020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.151"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JQH22_003596"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JQH22_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFB2207784.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3190)
                     /gene="hemX"
                     /locus_tag="JQH22_003597"
     CDS             complement(1967..3190)
                     /gene="hemX"
                     /locus_tag="JQH22_003597"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFB2207785.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="JQH22_003598"
     CDS             complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="JQH22_003598"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFB2207786.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="JQH22_003599"
     CDS             complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="JQH22_003599"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFB2207787.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5277..7823
                     /gene="cyaA"
                     /locus_tag="JQH22_003600"
     CDS             5277..7823
                     /gene="cyaA"
                     /locus_tag="JQH22_003600"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFB2207788.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="JQH22_003601"
     CDS             complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="JQH22_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFB2207789.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8231..>8652)
                     /locus_tag="JQH22_003602"
     CDS             complement(8231..>8652)
                     /locus_tag="JQH22_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFB2207790.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2236 c         2228 g         2220 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca
     2101 ggttacgcac gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg
     2161 gaagatccat cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg
     2221 tggtggcatc atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct
     2281 ggcgataagt ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc
     2341 gaatattttc gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat
     2401 cacgacggcg aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga
     2461 tacgccattc gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat
     2521 cgctgtcatt atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa
     2581 ggatgatgcc gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa
     2641 ttgcccgacg aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac
     2701 ttttcagcaa cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga
     2761 gtttcaccag aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg
     2821 tggcgatctt ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg
     2881 cctgcttaag ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct
     2941 cctgggcttt ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat
     3001 tgacggcctg ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga
     3061 tagccaccgc gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag
     3121 gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt
     3181 gttccgtcat tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt
     3241 tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca
     3301 aacgctcact gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg
     3361 ggatcagcga ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct
     3421 cgcgggattg ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat
     3481 aacattcaca aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac
     3541 gaccgccatt gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta
     3601 attgtagcaa gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta
     3661 cggtatgtag cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc
     3721 atttacgatc ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg
     3781 caaacaacag atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc
     3841 gacccggaga aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac
     3901 ggctcactaa ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc
     3961 ctctccgtta tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc
     4021 cagcgaaatc cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat
     4081 ctgcgaaccg tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc
     4141 ggcgtagcta ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc
     4201 tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg
     4261 tgtatcatca aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat
     4321 ctcgggtggt aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac
     4381 ggcaagaatg atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt
     4441 gccgcgcagg gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg
     4501 taaactggac gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt
     4561 ggacacaaag gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg
     4621 cgggaattca accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag
     4681 gagcgcgact tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt
     4741 atcaagaatc acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg
     4801 gctcgccatc aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg
     4861 tgtggcaatt cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc
     4921 atcctaacat ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa
     4981 accgtaaaat gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga
     5041 atttacagag aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc
     5101 agcaaggtgt taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc
     5161 aggcgcgaaa agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt
     5221 accgttgatg ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt
     5281 acctctatat tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc
     5341 gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt
     5401 tgcactatca ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt
     5461 gcctttacac gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg
     5521 gaatgtcagt acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg
     5581 gcagcacctc gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc
     5641 aatcctggct cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa
     5701 gctgggccgc ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc
     5761 gtcataatga aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc
     5821 tgcttgacga attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata
     5881 tggtgccgtg cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg
     5941 gcgtgctgac gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag
     6001 agtactttgg tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg
     6061 tgctgaaaac actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg
     6121 cgaaagatat caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat
     6181 actgcatgat gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc
     6241 tggatttagt acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg
     6301 cctgtgtagg ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg
     6361 aagctcgtct ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg
     6421 cgcacaacga gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc
     6481 gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac
     6541 tgtatgccgc gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac
     6601 ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt
     6661 caggttggta tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc
     6721 tggaatataa ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga
     6781 cctcgcgcac ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg
     6841 agatggtcgc cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg
     6901 cgctctacag cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc
     6961 cgacagctgc gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca
     7021 gctttggcga gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt
     7081 ggaacgaagt gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa
     7141 ctattctcgg caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt
     7201 atagccagca tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta
     7261 ttgaactgcg cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt
     7321 ctggtcaaac ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga
     7381 acgccatcga gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg
     7441 ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca
     7501 tccagttctt tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg
     7561 aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg
     7621 acgtcagtcg cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca
     7681 acttcaacct gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc
     7741 cgttccgaac aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc
     7801 tattacagca gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa
     7861 tcttagcgga aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc
     7921 cagaaggttt cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag
     7981 ccgccctgtt tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt
     8041 ttgctgccgt tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg
     8101 ctgtcgccat ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg
     8161 cgatgaaatt cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag
     8221 cggcatgggg tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat
     8281 ccgtcaccgg aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac
     8341 gagttttcgc cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa
     8401 cattgcgatc gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa
     8461 tttgcaagct ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa
     8521 atgagcgatc aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac
     8581 caaatgtcga taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa
     8641 tcagatggaa tt
//
LOCUS       DADLDT020000149         8490 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000149 DADLDT020000000
VERSION     DADLDT020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.152"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            44..397
                     /locus_tag="JQH22_003603"
     CDS             44..397
                     /locus_tag="JQH22_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFB2207791.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQH22_003604"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQH22_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFB2207792.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQH22_003605"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQH22_003605"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFB2207793.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQH22_003606"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQH22_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFB2207794.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQH22_003607"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQH22_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFB2207795.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQH22_003608"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQH22_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFB2207796.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQH22_003609"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQH22_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFB2207797.1"
                     /translation="MHEITLCQRALELIEQQAAKHDAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="JQH22_003610"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="JQH22_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFB2207798.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="JQH22_003611"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="JQH22_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFB2207799.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8490
                     /locus_tag="JQH22_003612"
     CDS             7649..>8490
                     /locus_tag="JQH22_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFB2207800.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLEN"
BASE COUNT         1902 a         2305 c         2286 g         1997 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgctg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgtc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 tatcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctggggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat
//
LOCUS       DADLDT020000150         8412 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000150 DADLDT020000000
VERSION     DADLDT020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.153"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="JQH22_003613"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="JQH22_003613"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFB2207801.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="JQH22_003614"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="JQH22_003614"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFB2207802.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="JQH22_003615"
     CDS             3115..3399
                     /locus_tag="JQH22_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HFB2207803.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQH22_003616"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQH22_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFB2207804.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="JQH22_003617"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="JQH22_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFB2207805.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQH22_003618"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQH22_003618"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFB2207806.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2139 g         2001 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gc
//
LOCUS       DADLDT020000151         8395 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000151 DADLDT020000000
VERSION     DADLDT020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.154"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            3..816
                     /gene="ttdA"
                     /locus_tag="JQH22_003619"
                     /pseudo
     CDS             3..816
                     /gene="ttdA"
                     /locus_tag="JQH22_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            813..1418
                     /gene="ttdB"
                     /locus_tag="JQH22_003620"
     CDS             813..1418
                     /gene="ttdB"
                     /locus_tag="JQH22_003620"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFB2207807.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1466..2929
                     /gene="ttdT"
                     /locus_tag="JQH22_003621"
     CDS             1466..2929
                     /gene="ttdT"
                     /locus_tag="JQH22_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFB2207808.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="JQH22_003622"
     CDS             complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="JQH22_003622"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFB2207809.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4223..4438
                     /gene="rpsU"
                     /locus_tag="JQH22_003623"
     CDS             4223..4438
                     /gene="rpsU"
                     /locus_tag="JQH22_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFB2207810.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4549..6294
                     /gene="dnaG"
                     /locus_tag="JQH22_003624"
     CDS             4549..6294
                     /gene="dnaG"
                     /locus_tag="JQH22_003624"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFB2207811.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6489..8330
                     /gene="rpoD"
                     /locus_tag="JQH22_003625"
     CDS             6489..8330
                     /gene="rpoD"
                     /locus_tag="JQH22_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFB2207812.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2252 g         1862 t
ORIGIN      
        1 cagtgaataa gttgacagag attgtcgcta actttaccgc catgatttct acccgaatgc
       61 ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacgtcg tcgatgggga
      121 aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc gattgacctg aatcgtcctg
      181 cctgtcagga caccggggag attatgttct tcgttaaagt cggttcccgc ttcccactgc
      241 ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga ggcaaccgtc aaagcgccac
      301 tacgtcacaa tgcggtagaa atttttgacg aagtaaacac cggcaaaaat accggtagcg
      361 gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga cgatgcggaa atcgaagttt
      421 acatggcagg cggcggctgc acgctacctg gccgctcgaa agtgttaatg ccgtcagaag
      481 gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc gtaaacgcct
      541 gtccaccggt actggtgggc gtgggcatcg ccacctcggt ggaaaccgcc gccgtactct
      601 cgcgtaaagc cattttgcgc ccgattggca gccgccatcc caatccaaaa gcggcagaac
      661 tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa gggctgaccg
      721 gcaacagttc agtgatgggc gcatcgtcgc ggcacgctgc tggttcatgc cgatctcacc
      781 tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa gatcctgaca accccgatca
      841 aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg
      901 tgacctgccg cgacgtttgt caccgccgtt tgatcgaact gaaacgtccg atcccttacg
      961 atctcaacgg caaagcgatt ttccacgctg gccccatcgt gcgcaaaaac ggtgacaaat
     1021 gggagatggt ctccgtcggc ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat
     1081 ttattgagca gaccggcgta aaactggtgg ttggcaaagg tggtatgggg ccgctgaccg
     1141 aagaaggctg ccagaaattc aaggcgttac atgtgatttt cccagcaggc tgcgcggtgc
     1201 tggcggcaac ccaggtagaa gagattgaag aagtgcactg gacagaactc gggatgccgg
     1261 agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct gattgtctct attgataccc
     1321 acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg
     1381 tggaagagat ctgcgagcac gtccattaca tcaaataacc ctcccggaga ggctcacccc
     1441 tctccttttt cgcaggcata acacgatgaa accttccact gaatggtggc gatatcttgc
     1501 gccgctggcg gtcatcgcca ttattgctct acttccggtt cccgcagggc tggagaacca
     1561 tacctggctc tactttgccg tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt
     1621 gccgggtgcc gtggtggcga tggtgggtat ctccatcatc gccatactct ctccctggct
     1681 gctgttcagc cccgaacagc tcgcccagcc aggctttaag ttcaccgcca aatccctctc
     1741 ctgggcagtt tctggttttt ctaattcggt tatctggctc attttcgccg cctttatgtt
     1801 tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa
     1861 gatggggcat cgcacgctgt ttctcggcta tgcggtgatg ttctccgagc tgatcctggc
     1921 acctgtaaca ccgtccaact cggcgcgtgg tgcgggtatt atctatccca tcatccgtaa
     1981 cctgccaccg ctctatcaat cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta
     2041 catcatgtgg atggggattg ttgccgactg cgtgaccagc gccattttcc tgacggcgat
     2101 ggcacctaac ttgctgttaa ttggactgat gaaaagcgca tctcacgcca cgctgagttg
     2161 gggcgactgg ttcctcggga tgttgccgct cagcatttta ctggttctgc tggttccctg
     2221 gctggcttac gtgctgtacc cgccggtact gaagtctggt gatcaggtgc cgcgctgggc
     2281 agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct
     2341 gatggtaggc gcgctggtgc tgtggatttt cggcggtgat tatatcgatg ccgcgatggt
     2401 cggttacagc gtggtggcgc tgatgctgct tctgcgcatt atcagttggg acgacattgt
     2461 cagtaataaa gcggcatgga acgttttctt ctggctggcc tcgcttatca ccctcgcgac
     2521 cggactcaac aataccggtt ttattagctg gtttggcaaa ctgttagcag gcagcttaag
     2581 tggttattcg ccgacgatgg tgatggtgac gttgattgtg gtgttttatc tactgcgcta
     2641 ctttttcgcc agcgccacgg cgtatacctg cgccctcgca ccgatgatga tcgccgccgc
     2701 gctggcgatg ccggaaatcc cgctgccggt gttctgcctg atggttggcg cggcaattgg
     2761 tctggggagc attcttacac catacgccac cggacccagt ccgatttact acggtagtgg
     2821 ttatctgcca acggcggatt actggcgact gggggcgatt tttgggctga tattcctcgt
     2881 gttgctggtg attaccggct tactgtggat gcccgtggtg ttgctttaag tgaggacggt
     2941 gccggaaatt gctctccggc acccaatagt tttacgcagc cggtaactcc gccagcggcc
     3001 agcgcggacg cacgctaaca ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc
     3061 cggcataggc gatcatcgcg ccgttatcag tacaaaattc cggacgcgcg tagaacactt
     3121 cgccgcggcg ttttttcatc atttcagcca gcttcgcccg taacgtgcgg ttagcactca
     3181 cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa
     3241 tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt
     3301 cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga
     3361 aatccagccc cggacggtcg gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct
     3421 gagccgccat tttcgacagt aacggtccgc caggataatc cagccccagc agcttcgcgg
     3481 ttttatcaaa cgcttcaccg gcggcatcat cgatagactc gccgagcagc tcgtactgac
     3541 caatgccagt cacgctgatt aactgcgtat gaccgccgga caccagcagc gcgacaaacg
     3601 gaaattccgg cgggttatct tccagcatcg gcgctaacag atgcccttcc atatggtgta
     3661 ccgggattgc cggaacgttc caggcaaacg ccagagaacg ccccacggtt gcgccaacca
     3721 gcagtgcgcc gactaatcca gggcctgcgg tataggccac agcatcaatg tcttttgccg
     3781 ttaaaccaga ctcctttagc gccgcctgga tcaacggtac ggttttacgc acatgatcgc
     3841 gggaggccag ttcaggcacg acgccgccgt agtcagcgtg caatttcacc tgactataca
     3901 attggttggc taacaaacct ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg
     3961 aagtttcaat acccagtaca cgcatgactt gttttacctc gctttattac cgcgcagtgt
     4021 aggaccaatg cgggttgatg taaaactttg ttcgcccctg gagaaagcct cgtgtatact
     4081 cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt gctttacaaa gcagcagcaa
     4141 ttgcagtaaa attccgcacc attttgaaat aagctggcgt tgatgccagc ggcaaaccga
     4201 attaatcaaa ggtgagaggc acatgccggt aattaaagta cgtgaaaacg agccgttcga
     4261 cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg
     4321 tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag cgcgctaaag cttctgcagt
     4381 gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt
     4441 ccccgagagc gttgctctcc gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa
     4501 ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat cggggcctat ggctggacga
     4561 atcccacgcg tattcattaa tgatctgctg gcacgcactg acatcgtcga tctgatcgat
     4621 gcccgtgtga agctgaaaaa gcagggcaag aatttccacg cgtgttgtcc attccacaac
     4681 gagaaaaccc cgtcattcac cgttaacggt gagaaacagt tttaccactg ctttggatgt
     4741 ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg acaagctcga gttcgtcgaa
     4801 acggtcgaag agctggcagc aatgcacaat cttgaagtgc catttgaagc aggcagcggc
     4861 cccagccaga tcgagcgcca tcagaggcaa acgctttatc agttgatgga cggtctgaat
     4921 acgttttacc aacaatcttt acaacaacct gttgccacgt ctgcgcgcca gtatctggaa
     4981 aaacgcggat taagccacga ggttatcgct cgctttgcga ttggttttgc gccccccggc
     5041 tgggacaacg tcctgaagcg gtttggcggc aatccagaaa atcgccagtc attgattgat
     5101 gcggggatgt tggtcactaa cgatcaggga cgcagttacg atcgtttccg cgaacgggtg
     5161 atgttcccca ttcgcgataa acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc
     5221 aacgataccc ccaaatacct gaactcgccg gaaacagaca ttttccataa aggccgccag
     5281 ctttacggtc tttatgaagc gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc
     5341 gaaggctata tggacgtggt ggcgctggcg caatacggca ttaattacgc cgttgcgtcg
     5401 ttaggtacgt caaccaccgc cgatcacata caactgttgt tccgcgcgac caacaatgtc
     5461 atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg catggcgagc gctggaaacg
     5521 gcgctgcctt acatgacaga cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa
     5581 gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag
     5641 gcgatgccac tctccgcatt tctgtttaac agtctgatgc cgcaagttga tctgagtacc
     5701 cctgacgggc gcgcacgttt gagtacgctg gcactgccat tgatatcgca agtgccgggc
     5761 gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca aattaggcat acttgatgac
     5821 agccagcttg aacgattaat gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg
     5881 cagctaaaac gcacgaccat gcgtatactt atagggttgc tggtgcaaaa tccagaatta
     5941 gcgacgttgg tcccgccgct tgagaatctg gatgaaaata agctccctgg acttggctta
     6001 ttcagagaac tggtcaacac ttgtctctcc cagccaggtc tgaccaccgg gcaactttta
     6061 gagcactatc gtggtacaaa taatgctgcc acccttgaaa aactgtcgat gtgggacgat
     6121 atagcagata agaatattgc tgagcaaacc ttcaccgact cactcaacca tatgtttgat
     6181 tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg agcgcacgca tggtttaagc
     6241 aacgaagaac gcctggagct ctggacatta aaccaggagc tggcgaaaaa gtgatttaac
     6301 ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc cgcactgaga ggcagcggca
     6361 aatatataag tacgccctcg taattatcgt tggcggtaaa caaccgttgg atttcagcgt
     6421 taacggctga aggacatcgg gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat
     6481 accgtcttat ggagcaaaac ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg
     6541 agcaaggcta tctgacctat gccgaggtca atgaccatct gccggaagat atcgtcgatt
     6601 cagatcagat cgaagacatc atccaaatga tcaacgacat gggcattcag gtgatggaag
     6661 aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg
     6721 aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat cgggcgcacg actgacccgg
     6781 tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg
     6841 acatcgctaa gcgtattgaa gacgggatca accaggttca atgctccgtt gctgaatatc
     6901 cggaagcgat cacctatctg ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc
     6961 tgtccgatct gatcaccggc tttgttgacc cgaacgcaga agaagatctg gcacctaccg
     7021 ccactcacgt cggttctgag ctttcccagg aagatctgga cgatgacgaa gatgaagacg
     7081 aagaagatgg cgatgacgac agcgccgatg atgacaacag catcgacccg gaactggctc
     7141 gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga
     7201 aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa actgtctgaa gtattcaaac
     7261 agttccgcct ggtgccgaag cagtttgact acctggtcaa cagcatgcgc gtcatgatgg
     7321 accgcgttcg tacgcaagaa cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc
     7381 cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg
     7441 cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc
     7501 atcgcgccct gcaaaaactg cagcagattg aagaagaaac cggcctgacc atcgagcagg
     7561 ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag
     7621 agatggttga agcgaactta cgtctggtta tttctatcgc caagaaatac accaaccgtg
     7681 gcttgcagtt ccttgacctg attcaggaag gtaacatcgg cctgatgaaa gcggttgata
     7741 aattcgaata ccgccgtggt tacaagttct ccacctacgc aacctggtgg atccgtcagg
     7801 cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg tattccggtg catatgattg
     7861 agactatcaa caaactcaac cgtatttctc gccagatgct gcaagagatg ggccgtgaac
     7921 cgacgccgga agaactggct gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc
     7981 tgaagatcgc caaagagcca atctccatgg aaacgccgat cggtgatgat gaagattcgc
     8041 atctggggga tttcatcgag gataccaccc tcgagctgcc gctggattct gcgaccaccg
     8101 aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct gaccgcgcgt gaagcaaaag
     8161 ttctgcgtat gcgtttcggt atcgatatga acaccgacca cacgctggaa gaagtgggta
     8221 aacagttcga cgttacccgc gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac
     8281 tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc
     8341 ggatcaggcg ttacgccgca cccggcacta gtctctctgc acaaacgcca ccttt
//
LOCUS       DADLDT020000152         8262 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000152 DADLDT020000000
VERSION     DADLDT020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.155"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            68..316
                     /locus_tag="JQH22_003626"
     CDS             68..316
                     /locus_tag="JQH22_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFB2207813.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="JQH22_003627"
     CDS             381..749
                     /locus_tag="JQH22_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFB2207814.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="JQH22_003628"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="JQH22_003628"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFB2207815.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="JQH22_003629"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="JQH22_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFB2207816.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="JQH22_003630"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="JQH22_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFB2207817.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="JQH22_003631"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="JQH22_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFB2207818.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="JQH22_003632"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="JQH22_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2016 a         2306 c         2013 g         1927 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttaac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc gc
//
LOCUS       DADLDT020000153         8208 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000153 DADLDT020000000
VERSION     DADLDT020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.156"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..304
                     /locus_tag="JQH22_003633"
     CDS             <1..304
                     /locus_tag="JQH22_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFB2207819.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2204
                     /locus_tag="JQH22_003634"
     CDS             276..2204
                     /locus_tag="JQH22_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFB2207820.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2188..2391
                     /locus_tag="JQH22_003635"
     CDS             2188..2391
                     /locus_tag="JQH22_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2207821.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="JQH22_003636"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="JQH22_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="JQH22_003637"
     CDS             3971..5476
                     /locus_tag="JQH22_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFB2207822.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="JQH22_003638"
     CDS             5513..5860
                     /locus_tag="JQH22_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFB2207823.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="JQH22_003639"
     CDS             5918..6946
                     /locus_tag="JQH22_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFB2207824.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="JQH22_003640"
     CDS             6998..7384
                     /locus_tag="JQH22_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFB2207825.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="JQH22_003641"
     CDS             7396..7773
                     /locus_tag="JQH22_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2207826.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8208
                     /locus_tag="JQH22_003642"
     CDS             7760..>8208
                     /locus_tag="JQH22_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2207827.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPI"
BASE COUNT         1898 a         1933 c         2539 g         1838 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ccaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 ggctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattga
//
LOCUS       DADLDT020000154         7951 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000154 DADLDT020000000
VERSION     DADLDT020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.157"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="JQH22_003643"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="JQH22_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="JQH22_003644"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="JQH22_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFB2207828.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="JQH22_003645"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="JQH22_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="JQH22_003646"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="JQH22_003646"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFB2207829.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="JQH22_003647"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="JQH22_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFB2207830.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="JQH22_003648"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="JQH22_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFB2207831.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="JQH22_003649"
     CDS             complement(6510..7202)
                     /locus_tag="JQH22_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HFB2207832.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7684..>7951
                     /locus_tag="JQH22_003650"
     rRNA            7684..>7951
                     /locus_tag="JQH22_003650"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2037 a         1907 c         2100 g         1907 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aattcatcag
     7561 acaatctgtg tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata
     7621 ccaagtctca agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact
     7681 tttaaattga agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg
     7741 caagtcgaac ggtaacagga agcagcttgc tgtttcgctg acgagtggcg gacgggtgag
     7801 taatgtctgg gaaactgcct gatggagggg gataactact ggaaacggta gctaataccg
     7861 cataacgtcg caagaccaaa gagggggacc ttcgggcctc ttgccatcgg atgtgcccag
     7921 atgggattag ctagtaggtg gggtaacggc t
//
LOCUS       DADLDT020000155         7916 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000155 DADLDT020000000
VERSION     DADLDT020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.158"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            237..740
                     /gene="dps"
                     /locus_tag="JQH22_003651"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="JQH22_003651"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFB2207833.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="JQH22_003652"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="JQH22_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFB2207834.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="JQH22_003653"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="JQH22_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFB2207835.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="JQH22_003654"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="JQH22_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFB2207836.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="JQH22_003655"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="JQH22_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="JQH22_003656"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="JQH22_003656"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFB2207837.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="JQH22_003657"
     CDS             7091..7351
                     /locus_tag="JQH22_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFB2207838.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="JQH22_003658"
     CDS             7616..>7916
                     /locus_tag="JQH22_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFB2207839.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DADLDT020000156         7859 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000156 DADLDT020000000
VERSION     DADLDT020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.159"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..86)
                     /locus_tag="JQH22_003659"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="JQH22_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            84..408
                     /locus_tag="JQH22_003660"
                     /pseudo
     CDS             84..408
                     /locus_tag="JQH22_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="JQH22_003661"
     CDS             complement(438..3350)
                     /locus_tag="JQH22_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207840.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="JQH22_003662"
     CDS             3353..3469
                     /locus_tag="JQH22_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFB2207841.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="JQH22_003663"
     CDS             3833..4675
                     /locus_tag="JQH22_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFB2207842.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="JQH22_003664"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="JQH22_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFB2207843.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="JQH22_003665"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="JQH22_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFB2207844.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="JQH22_003666"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="JQH22_003666"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFB2207845.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1761 c         1860 g         2122 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DADLDT020000157         7838 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000157 DADLDT020000000
VERSION     DADLDT020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.160"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="JQH22_003667"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="JQH22_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="JQH22_003668"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="JQH22_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFB2207846.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="JQH22_003669"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="JQH22_003669"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFB2207847.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="JQH22_003670"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="JQH22_003670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFB2207848.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="JQH22_003671"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="JQH22_003671"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFB2207849.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1782 a         2038 c         2110 g         1908 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagta
//
LOCUS       DADLDT020000158         7792 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000158 DADLDT020000000
VERSION     DADLDT020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.161"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..104)
                     /locus_tag="JQH22_003672"
     CDS             complement(<1..104)
                     /locus_tag="JQH22_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFB2207850.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JQH22_003673"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JQH22_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HFB2207851.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="JQH22_003674"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="JQH22_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFB2207852.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="JQH22_003675"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="JQH22_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFB2207853.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="JQH22_003676"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="JQH22_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFB2207854.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="JQH22_003677"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="JQH22_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="JQH22_003678"
     CDS             complement(3704..4195)
                     /locus_tag="JQH22_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFB2207855.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="JQH22_003679"
     CDS             complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="JQH22_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFB2207856.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="JQH22_003680"
     CDS             complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="JQH22_003680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFB2207857.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="JQH22_003681"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="JQH22_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="JQH22_003682"
     tRNA            6840..6927
                     /locus_tag="JQH22_003682"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7077..7358)
                     /locus_tag="JQH22_003683"
                     /pseudo
     CDS             complement(7077..7358)
                     /locus_tag="JQH22_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7640..>7792)
                     /locus_tag="JQH22_003684"
     CDS             complement(7640..>7792)
                     /locus_tag="JQH22_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFB2207858.1"
                     /translation="MRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQ
                     KKEYQA"
BASE COUNT         2115 a         1865 c         1691 g         2121 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccagat tataagtgca tgttattgtt taacatgcgc
     6961 ttttttattt ttccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     7021 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     7081 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     7141 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     7201 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     7261 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     7321 gcgagtcggt cagcctgttg tccggtgata agatgaaatc tatggtcacc cccgtttttg
     7381 caacactgat ttttgattaa tggttggctt gcttaaatct atccggcgtc tctgtgggca
     7441 aggatgcccg cgccccgatg agttccgcac ctgataatcc taaaaatccc ctcggcttca
     7501 gtgagccatt tcatctgtta ggtttttaat caggtcgatt tgccgttcat gtcatcaata
     7561 atcagtcttc gcaaaaccag aaaggtaact tgtatcttat tgtttaaaat aaactaattg
     7621 agttatatca gcatctatat caggcctgat attctttttt ctgagtagtg attgcccagg
     7681 caattcgagc cagtttattt gccagagcgc aggtaaccac attcgaatgc ttcttgttga
     7741 gttgctcccg aacccactca gcaagttttc catgcttgcg ttcaagtcgc at
//
LOCUS       DADLDT020000159         7780 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000159 DADLDT020000000
VERSION     DADLDT020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.162"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            54..587
                     /gene="ymdB"
                     /locus_tag="JQH22_003685"
     CDS             54..587
                     /gene="ymdB"
                     /locus_tag="JQH22_003685"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFB2207859.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            529..2010
                     /gene="clsC"
                     /locus_tag="JQH22_003686"
     CDS             529..2010
                     /gene="clsC"
                     /locus_tag="JQH22_003686"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFB2207860.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="JQH22_003687"
     CDS             complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="JQH22_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFB2207861.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3569..5104
                     /gene="mdoG"
                     /locus_tag="JQH22_003688"
     CDS             3569..5104
                     /gene="mdoG"
                     /locus_tag="JQH22_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFB2207862.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5097..7640
                     /gene="mdoH"
                     /locus_tag="JQH22_003689"
     CDS             5097..7640
                     /gene="mdoH"
                     /locus_tag="JQH22_003689"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFB2207863.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1926 a         1862 c         2058 g         1934 t
ORIGIN      
        1 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
       61 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      121 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      181 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      241 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      301 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
      361 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
      421 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
      481 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
      541 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
      601 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
      661 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg
      721 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
      781 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
      841 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
      901 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
      961 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1021 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1081 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1141 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1201 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1261 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1321 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     1381 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     1441 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     1501 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     1561 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     1621 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     1681 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     1741 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     1801 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     1861 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     1921 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     1981 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2041 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2101 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2161 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2221 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgtgct gactggaagt
     2281 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     2341 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     2401 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     2461 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     2521 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     2581 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     2641 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     2701 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     2761 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     2821 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     2881 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     2941 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3001 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3061 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3121 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3181 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3241 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3301 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     3361 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     3421 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     3481 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     3541 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     3601 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     3661 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     3721 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     3781 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     3841 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     3901 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     3961 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4021 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4081 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4141 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4201 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4261 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4321 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     4381 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     4441 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     4501 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     4561 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     4621 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     4681 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     4741 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     4801 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     4861 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     4921 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     4981 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5041 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5101 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5161 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5221 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5281 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     5341 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     5401 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     5461 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     5521 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     5581 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     5641 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     5701 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     5761 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     5821 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     5881 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     5941 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6001 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6061 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6121 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6181 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6241 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6301 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     6361 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     6421 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     6481 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     6541 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     6601 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     6661 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     6721 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     6781 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     6841 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     6901 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     6961 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7021 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7081 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7141 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7201 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7261 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7321 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     7381 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     7441 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     7501 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     7561 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     7621 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     7681 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     7741 catccggcaa aaacaacgag ccaataaaaa taccggcgtt
//
LOCUS       DADLDT020000160         7743 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000160 DADLDT020000000
VERSION     DADLDT020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.163"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="JQH22_003690"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="JQH22_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFB2207864.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="JQH22_003691"
     CDS             1102..2562
                     /locus_tag="JQH22_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFB2207865.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="JQH22_003692"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="JQH22_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFB2207866.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="JQH22_003693"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="JQH22_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HFB2207867.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="JQH22_003694"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="JQH22_003694"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFB2207868.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="JQH22_003695"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="JQH22_003695"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFB2207869.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="JQH22_003696"
     CDS             complement(6727..7245)
                     /locus_tag="JQH22_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFB2207870.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="JQH22_003697"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="JQH22_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFB2207871.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1812 c         1954 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DADLDT020000161         7719 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000161 DADLDT020000000
VERSION     DADLDT020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.164"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..830)
                     /locus_tag="JQH22_003698"
     CDS             complement(<1..830)
                     /locus_tag="JQH22_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HFB2207872.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="JQH22_003699"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="JQH22_003699"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFB2207873.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="JQH22_003700"
     CDS             complement(2479..2706)
                     /locus_tag="JQH22_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFB2207874.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="JQH22_003701"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="JQH22_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HFB2207875.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="JQH22_003702"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="JQH22_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFB2207876.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="JQH22_003703"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="JQH22_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFB2207877.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="JQH22_003704"
     CDS             complement(5528..6511)
                     /locus_tag="JQH22_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2207878.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="JQH22_003705"
     CDS             complement(6557..7381)
                     /locus_tag="JQH22_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFB2207879.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="JQH22_003706"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="JQH22_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFB2207880.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcact
//
LOCUS       DADLDT020000162         7410 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000162 DADLDT020000000
VERSION     DADLDT020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.165"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..639
                     /gene="bla"
                     /locus_tag="JQH22_003707"
     CDS             <1..639
                     /gene="bla"
                     /locus_tag="JQH22_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HFB2207881.1"
                     /translation="CGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVR
                     ELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDER
                     DTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSG
                     AGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     gene            849..1253
                     /locus_tag="JQH22_003708"
                     /pseudo
     CDS             849..1253
                     /locus_tag="JQH22_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1360..2196)
                     /locus_tag="JQH22_003709"
     CDS             complement(1360..2196)
                     /locus_tag="JQH22_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFB2207882.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2196..2999)
                     /locus_tag="JQH22_003710"
                     /note="aph(3'')-Ib"
     CDS             complement(2196..2999)
                     /locus_tag="JQH22_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFB2207883.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3060..3875)
                     /gene="sul2"
                     /locus_tag="JQH22_003711"
     CDS             complement(3060..3875)
                     /gene="sul2"
                     /locus_tag="JQH22_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFB2207884.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4205..4381
                     /locus_tag="JQH22_003712"
     CDS             4205..4381
                     /locus_tag="JQH22_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HFB2207885.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(4563..5567)
                     /locus_tag="JQH22_003713"
     CDS             complement(4563..5567)
                     /locus_tag="JQH22_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HFB2207886.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(5646..>7410)
                     /locus_tag="JQH22_003714"
     CDS             complement(5646..>7410)
                     /locus_tag="JQH22_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFB2207887.1"
                     /translation="LRAAPAAKDVLDAIEVLRGMNSDNARKVPADAPTEFIKPRWQKL
                     VMTDTGIDRRYYELCALSEMKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASE
                     LPLAVATDCNRYLNDRLTLLETQLATVNRMATANELPDAIITESGLKITPLDAAVPDT
                     AQALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAKDKNLLLTTILADAI
                     NLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQFRHPFAEHWGDGTTS
                     SSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVL
                     DGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAY
                     DALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALR
                     ELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQR
                     YRASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYL
                     WRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         1652 a         2026 c         2102 g         1630 t
ORIGIN      
        1 tgtggtgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
       61 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgctgccaac
      181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      301 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
      361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
      661 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
      721 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      781 gacccctaaa agaactttcc gctaagcgat agactgtatg taaacacagt attgcaagga
      841 cgcggaacat gcctcatgtg gcggccagga cggccagccg ggatcgggat actggtcgtt
      901 accagagcca ccgacccgag caaacccttc tctatcagat cgttgacgag tattacccgg
      961 cattcgctgc gcttatggca gagcagggaa aggaattgcc gggctatgtg caacgggaat
     1021 ttgaagaatt tctccaatgc gggcggctgg agcatggctt tctacgggtt cgctgcgagt
     1081 cttgccacgc cgagcacctg gtcgctttca gctgtaagcg tcgcggtttc tgcccgagct
     1141 gtggggcgcg gcggatggcc gaaagtgccg ccttgctggt tgatgaagta ctgcctgaac
     1201 aacccatgcg tcagtgggtg ttgagcttcc cgtttcagct gcgtttcctg tttggggtcg
     1261 tttgcgggaa ggggcggaat cctacgctaa ggctttggcc agcgatattc tccggtgaga
     1321 ttgatgtgtt cccaggggat aggagaagtc gcttgatatc tagtatgacg tctgtcgcac
     1381 ctgcttgatc gcggccgcga tagctagatc gcgttgctcc tcttctccat ccgcgttcca
     1441 agctgcggaa aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg gatcgacgtc
     1501 cagcgcacga gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga gacaaaggtc
     1561 gtctctgtca gccggatcgt agaacatatt ggcggcgcca aagcccactt caccgaccag
     1621 accgacggga tctatcacca gccagccgcg actggagaac atgatgtttt catgatgcag
     1681 atcgccatgt agcccacgca gttccgaggc attgctcatc atttgatcgg ctataatcgc
     1741 cgcgtggacg tagtcagttt gacaacctgc gttttgatca tcgcgcgccc gctgaaacaa
     1801 agctgcaaag cgatcccgga tcgggagaag ggcagaaggc aggggttcct cagatgcggc
     1861 atacagcttc gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc cgtgctcggc
     1921 aacgatgtga gagagcattc gctccccggc atattcgagc aacatcagat tgttctcacg
     1981 accgagcaac cggactgctc ccctcccatt gcgccatacc agatagtcgg ccccgcgcag
     2041 ttcatcagca atgtcttcta taggtttcaa tcccttgacg attgcaggag tcccgtctgg
     2101 caatgaaact ttccaaacga ggctggaaaa ggtgtccgca atgagaacag gttgcgaaac
     2161 gtgccaatga gcaggaaaaa caggcggcat gaacatcaac cccaagtcag agggtccaat
     2221 cgcagataga aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac attgaatagg
     2281 acagcgaagg cgcgctctgc ttcatctggc gctgcccagt tctcttcggc gttagcaatc
     2341 atgagtgcca aatcggcata gcgatctgct gttccgagcc gcccaaggtc gatcagaccc
     2401 gtgcattgaa gagttttagg gtccaccatg aagttcggca tgcagggatc accatggcaa
     2461 acaaccatat cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc gacacgagcc
     2521 aaaagatcga gctgcggcgt actcttgtcc tcgtccggta agaagtcggg attgacggca
     2581 ttgcgggaca ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg ctcaaacgga
     2641 cattgatcaa ccgataggct gtgaacagcg ccaagttgct gccccattga cggccacgct
     2701 ttgagcaaat ccgctccaga cagatcagcc gccggtactc ccggaattgc cgttatcacc
     2761 aagcatgcac cctcctgttc ctcctgccag ttgatgacct cggggcaagc cacacctcga
     2821 cctttgagcc aaatgaggcg gtcacgctct ccagcgagct caccgcggcg ggaagcaggt
     2881 gcgattttcg cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc agattctccg
     2941 cctctgacag gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt tcgattcaat
     3001 ggaggttcct tcagttttct gatgaagcgc gaatatagag aaatatcccg aatgtgcagt
     3061 taacgaattc ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg
     3121 cgcggctcgt gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg
     3181 agtgtcgcgg ccccgacatc ccccggacca cggcctgtga gcgcgcgcag aaaggatttg
     3241 cgcgaaacag acagaagcac cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc
     3301 agcaccgaga gcgaggtttc gggagcagcc cccagaaaaa accccatgcc gggatcaagg
     3361 acaaggcggt tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac
     3421 gccgcaatgt gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct
     3481 tgcaccgaat gcataacgac gagtttggca gatgatttcg ccaattgcgg atagaacgca
     3541 gcgtctggaa aaccgcgaat atcattgaga taggccacac cacgcgacaa ggcataggct
     3601 tgcgtcgcgg gttgataact gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc
     3661 agcaccggcg cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc gtcgggattg
     3721 ctggatgccg gaccgaggtc gatcacatct gccccctcgg ccatcagctt acgcgcctgc
     3781 gcaatggctg cgtctggcgc cagataccgg cctccatcgg agaaactgtc cgaggttatg
     3841 ttgacgatgc cgaaaatgat gagcgattta ttcatggggg cttctataat aataataatc
     3901 gagcatgagt ctcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt
     3961 tgcggtgctc cattagctgc aggagcagaa tagcatacat ctggaagcaa agccaggaaa
     4021 gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc
     4081 ttttctgagc atggtatttt tcatggtatt accaattagc aggaaaataa gccattgaat
     4141 ataaaagata aaaatgtctt gtttacaata gagtgggtat cggagacgaa gaaggacaag
     4201 gtacatgctg accgatacca agctgcgcaa tctcaagccc agggacaaac tctacaaagt
     4261 gaatgaccgg gaaggtctct atgtgggggt cgcctcagaa aacggaatct atggtcactc
     4321 ccgtttttgc aacaccgatt ttgacgacaa gttggcttgc ttgaatctat ccggcgtctg
     4381 aatgggattt tattcccgcg cctcgatgag ttccgcgcct gatgaacctc cagaaaatat
     4441 acggcttcaa tgagcctttc cgttttacag gttcctcaac aggccggtgg gccgttagta
     4501 tcatcaatat cagtattcgc aaaaccagat gaatgattgt ttaaactggt gtatttctgc
     4561 ctttatgctt cgtaagtttg ctgtcgcgcc gtcagtgccc aggctattct ggccagcttg
     4621 tttgccagag cacaggtgac gacaaagttg cttttccgac acaacaactc cctgacccag
     4681 tcggccaact tgccagactg gtgttccagt ttttgtatga ataccctggc acactgaacc
     4741 aacaaagttc ggatcttttt gttgccccgc ttgctaatcc ctaacaatgt cgtccgacct
     4801 cccgtgctgt actgtcgggg taccagccct gttgccgccg caaagtcacg gctgctggcg
     4861 tactgcttcc cgtcgccaat ctcagttgaa atagtactgg cagtcagcgt tccaacgcag
     4921 ggaatactca gcaagcgctg tccaacctca tcttcgtcca actttcgttt caactgagat
     4981 tccagatctt taatctgctc aacaagatag tgataatgct gttgtaattt cagcagtaac
     5041 tggctgagat aaagaggcaa actactgtcc tcaagaaggg tactcagtcg actaataacg
     5101 gcagcacctc gcggaacgct gataccaaat tccagcagaa aagcatgcat ctgattagtt
     5161 gttttcacct tatcctgaac cagggattca cggacacgat gcagagctcg cattgcctgc
     5221 tgagattcgg ttctgggctg cacgaaacgc atagatggac gtgatgctgc ttcacagata
     5281 gcttcagcat caacgaagtc atttttgttg cttttaacga atgggcggac aaattgcggt
     5341 gatatcagct ttggaaaatg ccctaactct tccagcttgc gtgccataaa gtgagaaccg
     5401 ccacaggctt ccatcgcgat ggttgttgcc gggcatgtcg ccagaaattc gattagcttt
     5461 ggtcgggtga attttttacg gtaaacggcc ttcccacgat gatcctgaca atgaatatgg
     5521 aaagagttct tacccagatc gataccaata agcgcaatgt tttccatgat ggttctccga
     5581 atgaaagcct gtcctcagca tagtactggg aaggagggag tgaccatctc attaaataaa
     5641 gcacgctaag ccggttgcag cggtcgtagc ggcctgaact tgcccgcgcc gatcttggcg
     5701 ctgctgcgcc agaggtaatc gccggtgagg ttgatgtgct cccaaccgag cggcgacagg
     5761 tactgcaaca gcgcgtcatc aacggcatgg ccgttgccac gcagcgcgtg cgcagcccgt
     5821 tccagataga ccgtgttcca caacacgacg gcagccgtta ccagattgag gccgctagcc
     5881 cggtagcgct gctgctcgaa actgcggtcg cggatttcac ccaggcggtt gaaaaacact
     5941 gccctggcca gcgcattgcg cgcctcgccc ttgttcaggc cggcatgcac gcggcggcgc
     6001 agttccacgc tttgcagcca gtccaggatg aacagcgtgc gctcgatgcg gcccagctcg
     6061 cggagcgcca cggccaggcc gttctggcgt gggtagctgc cgagctttcg gagcatcagg
     6121 gaggccgtca ccgtgccctg cttgatcgag gtggccagcc gcaggatttc gtcccaatgg
     6181 gcgcggacgt gcttgatgtt gagcgtgccg ccgatcatgg gtttcagcgc gtcataggcg
     6241 gcgtcgccct tcgggatgta gagcttggtg tcgcccaggt cgcggatgcg cggcgcgaag
     6301 cggaagccca ggaggtgcat cagggcgaag acgtgatcgg tgaagcccgc cgtgtcggtg
     6361 taatgctcct cgatccgcaa gtcggactcg tggtacagca ggccgtcgag cacgtaggtc
     6421 gaatcgcgca cgccgacgtt cacgaccttg gtgtgaaatg gcgcgtactg gtcagaaatg
     6481 tgggtgtaga acgtccgccc tgggctgctc ccgtatttcg ggttgatgtg gccggtgctc
     6541 tcggccttgc tgccggtgcg gaagttctgg ccgtccgacg atgaggtggt gccgtcgccc
     6601 cagtgctcgg cgaagggatg gcggaactgt gcgttgacca gatcggccag cgccgccccg
     6661 taggtttcgt cgcggatgtg ccaggcttgc agccaagcca gcttggcgta ggtcgtgccg
     6721 gggcaagact ccgccatctt ggtcaggccc aggttgatcg cgtcggcgag gatcgtggtc
     6781 agcaacaggt tcttgtcttt ggccgggtcg cccgatttca gatgcgcgaa atgccgagtg
     6841 aagcccgtcc attcgtccac ctccagcagc agttcggtga tcttgacgtg cggcaggatc
     6901 attgccgtct ggtcgatcag cgcttgggcg gtgtcgggta ccgccgcgtc gagcggcgtg
     6961 atcttcaagc ctgactcggt gatgatggcg tccggcagct cgttggccgt cgccatacgg
     7021 ttgacggtgg caagctgtgt ttccagcagc gtcagccggt cgttcaggta ccggttgcag
     7081 tcggtggcca cggccagcgg caattcgctg gcctgcttga ggctggcgaa tttcgcgggc
     7141 ggcaccaggt agtcctcgaa gtccttgaac tggcgcgacc cctgcaccca gatgtcgccg
     7201 gaacgcaacg cgttcttcat ctccgacagc gcgcacagtt cgtagtagcg ccggtcgatg
     7261 ccggtgtcgg tcatgaccag cttctgccag cgcggcttga tgaactcggt cggcgcgtcg
     7321 gcgggcacct tgcgggcgtt gtcgctgttc atgccgcgca gcacctcgat ggcgtcgagc
     7381 acgtccttcg cggcgggagc ggcccgcagc
//
LOCUS       DADLDT020000163         7183 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000163 DADLDT020000000
VERSION     DADLDT020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.166"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(296..1198)
                     /locus_tag="JQH22_003715"
     CDS             complement(296..1198)
                     /locus_tag="JQH22_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFB2207888.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQH22_003716"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQH22_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFB2207889.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JQH22_003717"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JQH22_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFB2207890.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JQH22_003718"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JQH22_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFB2207891.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JQH22_003719"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JQH22_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFB2207892.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JQH22_003720"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JQH22_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="JQH22_003721"
     CDS             7090..>7183
                     /locus_tag="JQH22_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFB2207893.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1673 a         1811 c         1878 g         1821 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DADLDT020000164         6951 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000164 DADLDT020000000
VERSION     DADLDT020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.167"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="JQH22_003722"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="JQH22_003722"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFB2207894.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="JQH22_003723"
     CDS             complement(1117..1575)
                     /locus_tag="JQH22_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFB2207895.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="JQH22_003724"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="JQH22_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFB2207896.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="JQH22_003725"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="JQH22_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFB2207897.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="JQH22_003726"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="JQH22_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFB2207898.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="JQH22_003727"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="JQH22_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HFB2207899.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="JQH22_003728"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="JQH22_003728"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFB2207900.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1833 a         1524 c         1700 g         1894 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gtgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctcct ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagctcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa agttgctttg ggtaaacaaa
     6841 aaaacggccc cagaaggggt aatgccagtc agttaagcaa ctgactggct ctttttcgga
     6901 gctgtggggt atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DADLDT020000165         6690 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000165 DADLDT020000000
VERSION     DADLDT020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.169"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(220..600)
                     /locus_tag="JQH22_003729"
     CDS             complement(220..600)
                     /locus_tag="JQH22_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFB2207901.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(593..793)
                     /locus_tag="JQH22_003730"
     CDS             complement(593..793)
                     /locus_tag="JQH22_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFB2207902.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(888..1145)
                     /locus_tag="JQH22_003731"
     CDS             complement(888..1145)
                     /locus_tag="JQH22_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207903.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1142..2533)
                     /locus_tag="JQH22_003732"
     CDS             complement(1142..2533)
                     /locus_tag="JQH22_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFB2207904.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2530..3408)
                     /locus_tag="JQH22_003733"
     CDS             complement(2530..3408)
                     /locus_tag="JQH22_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFB2207905.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3419..4327)
                     /locus_tag="JQH22_003734"
     CDS             complement(3419..4327)
                     /locus_tag="JQH22_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFB2207906.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4314..4547)
                     /locus_tag="JQH22_003735"
     CDS             complement(4314..4547)
                     /locus_tag="JQH22_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207907.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4544..5560)
                     /locus_tag="JQH22_003736"
                     /pseudo
     CDS             complement(4544..5560)
                     /locus_tag="JQH22_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5492..5755
                     /locus_tag="JQH22_003737"
     CDS             5492..5755
                     /locus_tag="JQH22_003737"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFB2207908.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5745..6053
                     /locus_tag="JQH22_003738"
     CDS             5745..6053
                     /locus_tag="JQH22_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2207909.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6095..6271)
                     /locus_tag="JQH22_003739"
     CDS             complement(6095..6271)
                     /locus_tag="JQH22_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFB2207910.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6268..6678)
                     /locus_tag="JQH22_003740"
     CDS             complement(6268..6678)
                     /locus_tag="JQH22_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFB2207911.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
BASE COUNT         1611 a         1799 c         1555 g         1725 t
ORIGIN      
        1 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
       61 tcatctgacc accagcgtgt ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc
      121 cggaggcggg tttaagctgt gtggcgaagc aaccactctt aacagaatat cccgtttttt
      181 acgtacgtaa aatattttct agaaagtcgc cccttaccat cagggatgtt caatatattt
      241 gtccatttct aaccggacac ccaacatcat caacattcct tccaccaccc cctcggcttt
      301 ttgtagcttc tttcctatat aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa
      361 tgtcatccca aacacgtagt agtcaaccag caagtcatgt agatcgttgt tgttccggtt
      421 aaggcgggcc atacacccac atataaccat cgcgtcatcg tcacagcatt gcgggcggga
      481 ttttactttt gaagggatta atcccttaaa accggcagca atggacgacc aggtcacatc
      541 ctcatgatta ttagccaccc acgcgcccca gcgctcaaga acctgctgaa tatcacgcat
      601 cactgtcttt acccctgtcc catccacgat gaacaatcag aacaccatca acaatggcgt
      661 gccctttgcc ctctttatct tcagcatatt ttcttaccgt ggcgcgatta cagttcagca
      721 ttcgtgaaac ttcggtcata ttacctcgtg tctggataag cagttccggt atcgtttgaa
      781 ttttgacgct catcaaatac tctccggttc ggtgattttt atcccgggct ttccaccagg
      841 gacataatga tcgcgcacaa gccagggcaa aacaaactca tggcgcatca gcgcagcgcc
      901 tcctgcacca gtttttcaaa ctttccgact ctggtttcca gctctgccac acagtccacc
      961 agctcatcta ctgctttctg tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt
     1021 accatatctt tacggatagc atcttttgtt atgcccgttt tttcgagttg ttcagcatga
     1081 cgcagcattt cctgcgcgtg tttacgcaat tgttcagggg taaaagtcat tgtctggttg
     1141 ttcaaaagaa acgctccatc ttactgctgt cagttcgttt attactgtat ctgcgcggat
     1201 tgccgggctt catgggagtg gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat
     1261 gtccgttata aaaacgccgg taaatcgttc ccagagaacc gttacgttgt ttcgtgatat
     1321 tgatttctgc gatgcccctg gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca
     1381 tcagaatgat gtctgcatcc gcctctattt ctccggaatt tttcaggtct gagttcatgg
     1441 gacgtttatt gggtctggac tccacaccgc gggagagctg gctcagcgca atcaacggaa
     1501 aaccaccgga ttttgccagg cctttaagcc cctttgagat ttcacccacg gcaaggtcat
     1561 gacgccccgt ggttcgggtt tttatcagcc cgagataatc aaccaccacc agcgccgttt
     1621 ccggatattt aatcaggtgg tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg
     1681 cctggtccac catccagata ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg
     1741 tccagtcttc atcttcaaag tgagccacag atttcaggcg tgatactggc atccctccag
     1801 ccgcagacac catacgttca ccaatctgga tgttcgccat ctccatggtg aacagaagca
     1861 caccatgccc ctgctcagtc accttgtcga tgatgtccag cgcaagttcg gttttcccca
     1921 tcgaaggacg ggcggcaatg aataccaggt ctccgggctc cataccgcct gtttttgcgt
     1981 ccagttcatc aataccggtc atcaacgtcc tggatttctc cagcccctga ttccggcatt
     2041 caacacgctc aaccacttcc ggaagcacat catcaatatg taccggctga atgacgccct
     2101 ttcctgtcga cagtgtgacc atcatgttct gcgcatcctt cagggcatct tcagctgctt
     2161 cacaggtatg cgcatcacgt aatttctgca gcgcctcatt cagtgttttt tctgcatcgc
     2221 gcagtgcggc attgcgccgc aacgctgcaa catagtgctc cagtgaagac ttcacccagg
     2281 ttttacgccc ggtatcagta atcaccgggg caagttccgg catctcatta cacaacagca
     2341 cggggtcaat cactcctgaa acacgggcct gtctgcagat gcctgtatag atatcctgat
     2401 acgctcgtac agaaaaaacg tccgctggca gtgtggccag aatatccatc acttcaggat
     2461 ctgctccgcg cagaaagaac gcgccgatga cagcgccttc aaggtcatcg ttacgccata
     2521 ctggagttgt catgcagcca cacctctgat acgagaacgg taactgggcc agttaaacga
     2581 caaccagttg cgtcccccgt ctgtgatcct gtcggcaatt cggggactga tgaacgccca
     2641 caactcttcc ggtgagaggt tactgataag aatggtgggc aagatacttt cgtacctggc
     2701 gttgataatt tcctgcaaaa tagccatttc agccgcgctg ccaaactgaa cgccaacttc
     2761 gtcgatgatc agcaaatcca ttgacgcata acgctcaata acttcatccg ctgttttttc
     2821 gctgtcattc cgccagcaat ttttcacagc acgggtaagg cgcatcacgt cggtgatctc
     2881 cacactggcc agatagttac ggatgatgtg ttttgccata gccacagcca gatgattttt
     2941 tccggtacca caactgccgg tcataacaag actggtaccg ttctccagca tatctggcca
     3001 gttctccgca tagcggcgac aggccgcaag atttctggct gcgccaggat taacctccag
     3061 ataattatca aactcgcagt cccgaaaacg cagggcaatt ccggcgttat cagtcagctc
     3121 ttccgccttg atggacgaca gctccatggt caaatcgttg gcctcagcga ttaagcagtc
     3181 agggcagcat gaaatttctt ctctgtcctc gccattacga tcgctccaca ccagtatatg
     3241 cgtgtgatat tcgccatgtt tttcgcaata tccgcgacct tcacgcatca ggcaggaacg
     3301 ataaggccat ggcttttcgc ccttctgagc aaatgcaatc tctgcccgta actcatccat
     3361 tcgcgcctgt agtctttttt gttgttcacg caggttaaac gtcatcatcg ctgtcacctc
     3421 agaatgtcag tttgtcactg gatttaccga atttgtcaga catggctccc aggccagcca
     3481 ggacatcgac ctgtcgctgt cgcccacctc tgggagcggc tggctgttgc cagaagtctt
     3541 cgaagtgacg atcgggtcca aagaacgtcg cagcctgctt cacgaactgt gtgccggtat
     3601 ttcctgtggc acgtacccag gcagcatagc gtttcacgcc atcaagcatg gcttcaggtt
     3661 ttattccctc cctgagccgg gctttccagg ctttgaaggc tgcggatttt gagttaccac
     3721 cagcacgttt tggatattcc tgccaggcct gttcaaattc cggtgaatat tcctgtcggg
     3781 aagaacgcgc tggtgcagac gcgtcagcgg atgcgccaat agtgttttta ctctctgtag
     3841 tattctctga agtaatctct gttgtattct ctgtaagatc gaaattggtt ttcccttctc
     3901 cgcggcgagg ggtttcccgt gtccgcggtg aaggctttcc ctcctccgcg aaattgggtt
     3961 ttacagtttc ccgaaaacgg gtttccccat ttcgggaaaa ctgattgttt tcattgataa
     4021 tttcattaag gcgctcacaa tctatacggt agaacatttt gtgctcaaga cgcttgttgg
     4081 tttcaaccaa aatgcctctg gacacaagat gcttacgcgc tacagcctgt tgttcaaatg
     4141 taagtccggt ttcgtgttgt atctcttcac gcgttttatg tacgccttcc gctgcatgtg
     4201 ctttatcctg ccagtaaaaa atctgaccaa agaaaataac agcgtgcgga cttcccatgt
     4261 atttaacgag cccagggtaa taagcaaccg gatgtccaaa atcgagcaga agatcagacg
     4321 gacgcatagc cacctcccag gcgtttaaac atttttccgg gctgaaacgc caccagcgga
     4381 taactcaggg tatgagtacg tccctgaacc tggcagacaa ccttctggct ttcggtactg
     4441 accaggcaaa cccgcagaac gtagccgttg ctggtggtga accactgccc cacacggggg
     4501 caatggttgt atcggtgata caggaaatta acgatgtggc ggatcatggg cgcacctcct
     4561 tgtcagaacc attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac
     4621 ggtcgtacag tttgccagcc agcggagatt caacggcctt aagcattggg taaagctggc
     4681 ttgtccagat ttgatggatt tcacgcaaat gcaggtatac gcctctggcg ttttgtgcga
     4741 cagatgacat gtcagccgca ccagctcctg ataagctcct ctccatctgg ttaaaggcat
     4801 tgatgtatgc ctctttgaac cgggcggcac gtttaccagt gaagcccata gcaaggaagg
     4861 cgaagccgcc gcgggtaatt tggtagcagg gaagtttgcg agtaccgccg ttgggctggc
     4921 gtaccaaaat tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa
     4981 tgcggatctt tttcagaaca tcgtcatgac gtttagagaa gaagttggca acagccaggg
     5041 atgaagtaac agcctaacca tcaacgatgg caatttcagg ttgagagagg gttgggagag
     5101 tagtcatggt gacagcccct atgttgaatt caaagaactc accacatggg acgccaatca
     5161 cagaggtggt gagacgtaca gggttggcgt aaccggtcaa catagaaccc ggcgcatctt
     5221 gcgatgtccc tgcacgcccc accataattt gggcgtagca atgctcatga cacgaaaaaa
     5281 ccgcatgagc gcggttatgc tctatattga atttcaggac gccaatcccg gcccccgctt
     5341 tataaggtgc agagacagtg taacgtcccg aaattgcaga atcaatatat tgtgcagata
     5401 taacgatttt gttatgtttt gctgtatgaa ctacactatc gaatactaca gtgaagaggt
     5461 ccgactggag gtcgatcagc ttccattgag tatgcgtgcc agataccagc atcttgttga
     5521 acgcatgaag gtatacggca gcaatctcgg agaacctcac accagcgcct ttggtgacgg
     5581 gcttttcgaa ctcagaatta aaggcaacga tgggatcgcg cgtgtttttt actgcactct
     5641 gacagggaaa cgcatcatca tgctgcatag ttttgtaaag aaagcgcaga aaacaccgcc
     5701 agccgaacgc aagaaagctg aaaccagaat gaaggaggtt aagcatgact ggtaaacgca
     5761 ctctccccac catgacacac gacgaaatgg cagccaaatg gatggaagac ccggctttta
     5821 aagcagaata cgacgctatc gctgacgaat tcgcactgct tgatgaaatg ctggcagcac
     5881 gcaaagaagc tggcttaact caggctgaag ttgccgagcg aatgggaaca aaagcgaccg
     5941 cgatcaccag aatggaaagt aatctcgcat caggtatcag cggcccatca tttgccacac
     6001 taaaaaaatt cgcccgcgct actggaaaaa aactccagat ccgcttcgtt taaccacacc
     6061 gcgccgtcat tctggcggcg caccggataa atagtcattt cctcgcacga tgtcttagcc
     6121 accggatatc ccacaggtga gtcgtgtagt tgaaggtttt tacgtcagat tcttttggga
     6181 ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt tcgcagatga
     6241 tgtcggtgaa acttcgtcga tgtcgcctca ttcatacctc ctgtcggtaa atctgacacc
     6301 ctgaccaata gcccaggctg ttgtgtactc gatcagactt gccatgcgct tcacgctcat
     6361 ctgcgcgctg ctttcgcgaa tgttgacgca ttctccttca aggccgggca aaacatcagc
     6421 ttcctgtttt gttgccactg catgaccgct gatcaacaaa accttccatt gttctggttt
     6481 taaccatttg ccgcaccatt gaacctgacg agcgatatcc gccagcatcg cgtgaaattt
     6541 tgcgttctgg tcaagatttc gcttgtagtc agtaatgcgg atggtgactg gcttgtcttt
     6601 atcgagagga gttgagagga tggcatttat tgcggcttgc tgttgttgct tagttcggag
     6661 gaagattgtt tgcttcatcg aaaattcttc
//
LOCUS       DADLDT020000166         6687 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000166 DADLDT020000000
VERSION     DADLDT020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.170"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="JQH22_003741"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="JQH22_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFB2207912.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH22_003742"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH22_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFB2207913.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH22_003743"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH22_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFB2207914.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH22_003744"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH22_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFB2207915.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH22_003745"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH22_003745"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFB2207916.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH22_003746"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH22_003746"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFB2207917.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH22_003747"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH22_003747"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFB2207918.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JQH22_003748"
     CDS             complement(4598..5320)
                     /locus_tag="JQH22_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFB2207919.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQH22_003749"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQH22_003749"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFB2207920.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..>6687
                     /locus_tag="JQH22_003750"
     CDS             6520..>6687
                     /locus_tag="JQH22_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFB2207921.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQ"
BASE COUNT         1647 a         1703 c         1604 g         1733 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccag
//
LOCUS       DADLDT020000167         6670 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000167 DADLDT020000000
VERSION     DADLDT020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.171"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(152..2155)
                     /locus_tag="JQH22_003751"
     CDS             complement(152..2155)
                     /locus_tag="JQH22_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207922.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2152..3288)
                     /locus_tag="JQH22_003752"
     CDS             complement(2152..3288)
                     /locus_tag="JQH22_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFB2207923.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3281..5560)
                     /locus_tag="JQH22_003753"
     CDS             complement(3281..5560)
                     /locus_tag="JQH22_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207924.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5571..6659)
                     /locus_tag="JQH22_003754"
     CDS             complement(5571..6659)
                     /locus_tag="JQH22_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFB2207925.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1530 a         1834 c         1787 g         1519 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat taccaactgg
       61 tgactctcac caggggctat ataggatatg cctaataccg tggcgtgagc agtccggaac
      121 tggagtagaa ctcttagtaa aaagcactat ttcatccttg ttgctgaagc atggggaata
      181 attgttcgca aagcaaaaca ccgttattca ttgcttctac ccgtgcctcg ctttctgtat
      241 tacgaaattg tgccaacaca tgtgccagcc gataaaaacc caccgcggtg aggtcattcg
      301 ccagcaactc tgcctgatta atagcgctct gttcctgata gcgccagccg ttatggagca
      361 gttgaataag taacgcctgg cagcgcatca gcaactgatg agcagtagac ggcacaggca
      421 gaacgctggc agaagatagt gataccacca caggcgcagt ttctgcgtcc agcgcccagg
      481 cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa ttgcccatca acaaaactga
      541 caaagccaga aaccagacac acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt
      601 caatggcata aggcgcgctg gctgaggctg gcagtgataa cgtcagcaga ttatcttcac
      661 cttcgccgct gattacctgc gcatccagcg tctggcggct gctatcccaa ccgagcgaaa
      721 tacactcagc gaccggcaga ataaataagt tatcgacctg attaagaggc cgtatgcagg
      781 cggggggacg ctggcgtaaa tattcccgca aagccacaat gcccggctgg cgtaacggcg
      841 cgctcaacat ttgccaggca tcaggcgaca gcggtacaac gctgcttaag cggttgcggg
      901 tagctaacag cagctcgcca tcggcactgc gttttgctgc ttgtgaaaca atttgcccac
      961 ccgccagtgc gccagcctga aaactaaaca gccgacgcgt agctgccggt gagtcttcct
     1021 gttcacttcg cggccaactg cgcgaaaggt gcaaaatact gccggtgtcg ggatcggtaa
     1081 accagatgcg taaaccataa tgctcaatat cctgccagca acgcatacct aaagacacca
     1141 gccgcagatg atcaagcttt gcttctccgg caatgccaga gccaacgacc gtgcgccacg
     1201 gcacaggagg aacttcacca acactgtcgc tccgggccat ctcttgtgcg caatttaatc
     1261 gactgtttaa tgccgcaagc tgacgtaagc attctccggc atgatagtgg ctggcgcggg
     1321 cgtggaaggc atcaacgctg gcgcgcagtt gccgtagcga ttcactcacc catcgccagc
     1381 tgcagcgttc cgctgcttgc tgcgcgcggc tgaaagcggc ctcgtagtga ataagcggct
     1441 ggctgatgcc gctcagccat aatgcctggc ttagttgttg aacatattga cgacacgttt
     1501 tgccttcttc gctggcaaac ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc
     1561 agattaaatg agtcaattcc gcttgctgcg cttttgcctg gacaaaggcc tgtactgcca
     1621 gtacgacatg ttcgcaaagt gtgccttcaa tacaatcaca acgggcgaaa cgaatactgc
     1681 tgcgggaata aaaacgcaca tcgctcatcg gtaagcgggc agagggaatt tcacccggcg
     1741 cacagaacaa ctcaatggtg atgcttttag cgaccagcgc ctgtgcgcgt ttgcgggtgg
     1801 catcgggaag ggtagccagt tcttccagcc agattgccgg atcccattcc tcttcttttc
     1861 ccgtaggctg ggcggtagca caaagtcgtt gataacttaa caccagcatc acgcgatgac
     1921 ggcacatgcc gctggcccca taggtgcact gagcctcttt cagtgcctgg ccgttcgcca
     1981 gctgggtacg gacaccgtca ctgaaggtgg cgattaaagc gctgttctca tggctgattt
     2041 ccgggacgtt gccattttcc agttccttaa ggctgcgctt aacaaaaccg gcattgctta
     2101 acgccgtcag tgcctgcggt gtcagttcta ataattccgg acgtagtgaa ttcatgactg
     2161 aagattctcc gcaagccatg atgccagctc gcccggtgtc atggcggcta tttgtgcgcc
     2221 gacattaatc agcgcctggg ccgtatcgcg gtcatagcaa ggtgttgctg tgctatcgag
     2281 cgctgccagt cccagcactt tgatgccgct ctggacacac tttttcactt ggtgcgtcag
     2341 caatgatgat gaaccccctt cataaaaatc gctcacgagg ataatgacgc tttttgcagg
     2401 ttgttcaata agttgccgac catactccat ggcactggcg atattggttc cgccgcctaa
     2461 ttgcactttc attaataact ctaccggatc ggcaacgtct gccgtgagat caacgacgct
     2521 tgtgtcaaac gccaccagat gggtacgaat gccgggtaac tgccataaac aggccgccat
     2581 caccgcagag tggatcaccg aatcgaccat cgatccgctt tgatcaacca gtaagaccag
     2641 ttgccattgc tcgctttggc gtttaatgcg gctgttaaag cgggggaatt cgatatacaa
     2701 cttgccgtgt tgcgggtgcc agtgttgcag gttggcgcgc agagtacttt tgaaatcaaa
     2761 gtttcgcgcc agtggaataa atgagcggcg acgacgatcg cggacaccag aaaaagcctg
     2821 acgaacttcc tttgccagtc gcgccataat ttcttccaca acctggcgca caatcctgcg
     2881 ggcggcagcc agtacttcgg ggttcatcag atgtttggtg tgcaaaacgg cgcgtagcag
     2941 gctttcggaa ggctgcatac gttccagcac gtcgagattt gtcaccacat cttcaatgcc
     3001 gtagcgcagt acggcatcgc tttccagccg ctcaatcacc tgttgcggaa acagcgtgtg
     3061 aatactgttg atccactcag gagtggtgag atttgagcca cctaatccac tagagcgttc
     3121 accacgctgg agccgttcag gatcgcgccc atacagccac tccagcgcgt gatctatctg
     3181 ccgggcgttg tcatccagcc cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg
     3241 ccagcgttgt agctcacggg tggtcagaag atcgttcagt tcagacatgg aaaactcccc
     3301 agtgttgcag cgatgccagt gcctgctgtt cgagttgttg atgatgtgca atggcttgcg
     3361 gtggacaatg caacggcatt tgcagtgccg aaacgggaag ttgcgccagt tgataatgct
     3421 caagcacctg atgcgccagc gtcccgcgtt ctcgtggcgg tagccaggcc attgccgcgc
     3481 gtaaatcggg cagggcattg gtaaaatcgg catcacttag ttgatttaaa tgactgctga
     3541 aaccggcgat aaatgccggc tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt
     3601 gcagcgcctc gccggattgt gccggggata actgcgccag catcgtcagc gccgcttcgg
     3661 cactagcgtt gggatgttcc agacgcatca gcgcaccaag agtcgcgccg cgatccagtg
     3721 ccaggacatg aatcgcctga ctgtgtcgct ccagcaaggc taccgccgca gaaagcgaaa
     3781 cgccggggag ttgcacaccg ctatgtagat cgcgcaggat atgacacaac gcttgccagc
     3841 tatgcaagtg agcgtgaaac tccttttcat ccggtctgcc gttggattca cacagccaca
     3901 gcgtgcgatc gatagccgcg cataacgtcg tttgtaatat ctgcgcgcct tgcataccgc
     3961 taatttcatc cagccgccat aaggcatata gcacttccag cgcctggccc atttcggcaa
     4021 attgattttc ctgggcgatt aataatgtga gttgctccag taattgttga ctgaaggacg
     4081 ccagacccgc tgacgccgct tggctaagac atgtggtaat gctgccgatt ccacccgcgt
     4141 ccagcatatc ggcttctaat ttatggcgtg cggcttcctg gagtgtggca ccaaagcagg
     4201 cggcctcaat caatgccgca tgctggctaa gcgggcgggt taatttccag cgttcttcac
     4261 cgttgcctgc aagtgtcagt gtgcttccct gctggcgtac aatccctggg atctccagta
     4321 ttgccagccg atgtaacacc tgactttgcg ctagtccatt ggggttaaag cgatttaagg
     4381 taagctccgc cggaagagag attgcggtac gttccagttc gcaagtgaca tcttttggca
     4441 gaggcggttg tggcgtagaa ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta
     4501 tcaacgttag cagaatcgga tcggtatcgg gatgaataac gccgcgatag ctccacggca
     4561 acggcgcatt cagggcttct ttaatcagcg atcctgctat ggcatccagc cagtcagtgc
     4621 gtaacggtag tgtatgaccg cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag
     4681 ccatatccgc ggtcgaagca ggcaacttgt gctggcgcaa acgggtgaga accgttttta
     4741 gcagttgttc tcccgcctgc tgtaagcccc actgccagca ccagttttgc cagaccgggg
     4801 caggcattcc tgaaaggtat cctgccagca catcaaggcg cttttcactg tagggggtga
     4861 gataacaacc tgtaacggca tctgccagcg acggcaattc tggcgtgtta attacctgcg
     4921 gacattcgcg ccacattttt gccagtgccg gagcgtgcca gccaccgcag acaactaaca
     4981 cgtcgccatt attttgctgc atcgcccatc caatccagcg ggccataaag gcttcgcgct
     5041 gacgatttag agcatcgccg ggggagtcgc cccgcagttg ggcaaaatag tgcgccagtg
     5101 cagagggtaa tgcagtttgc tggctttcat cttcgaacaa gtgatcccac agggtgtcgc
     5161 tgttatccat gcgggtggca cgcagcagca aggtctggct ttcctcttgc gtatcaggcg
     5221 aatcgtcctc ttcttcactc tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct
     5281 gaatacgacg atcggcttgt agcgcctgcc actccggcga aaattcagca aatggcgtcc
     5341 aggcaccacg cccgggggct gcaccgtcct gatactggca ataactgtaa atagctaccg
     5401 gaagctggtg ggctaaaaac agttcgtcta cccggtcatt aaaatcagca gggccttcaa
     5461 tcaacacgta tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg
     5521 gactatgatg ccggatgccg acaattaacg gctcgctcat gcatctttcc tcacggcagg
     5581 cggtggcggg cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt
     5641 tcgaagtaac ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca
     5701 atgcagtcca ccaggtcggc tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg
     5761 cctacggcgt gggccacgtt cacggcttca gcggtggaca taatcgctgt tagtgtatcc
     5821 attgacgttt tcttttcgcc gttggcacgc agatcgcgga aggtgcggac cagtaactcc
     5881 agtaccgcgt caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca
     5941 ctggcgacaa gctccagctc ctgggcgaaa tccataatcg gaaagacagt ttcaaaatcg
     6001 aagcgacgct ttagcgcagc actcatttca ttgacgccgc gatcgcgggt attggcagtg
     6061 gcaataatat taaagccttc tcgcgcatag agctgactgg cttcaccagt gagttccggc
     6121 accgtcatca ccctgtcaga aagcatacct aacagacagt cttgcacttc cagcggtgta
     6181 cgcgttatct cttcaaaacg gacgattttg ccgtcgcgca ttccctggta aagcggggca
     6241 gggaccagcg cttccgttga tggaccatga ttgataagca gggcgtagtt ccagccgtac
     6301 ttgatttgat cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta
     6361 atagaggttg ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac
     6421 atcaaaccgc gtccggtggc aagagtgact aacatacgtt cgacagcgga gggactaatc
     6481 accactttac ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcaggc
     6541 aaacttaact gccaaccggg cgggcaaggt gcgttatcat tttgttttaa ttttgccagt
     6601 tcatcggcgt ataacacagc agcaggcgga cgctgcagat gattattctg cggtgacata
     6661 tattcatccc
//
LOCUS       DADLDT020000168         6617 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000168 DADLDT020000000
VERSION     DADLDT020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.172"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            62..2494
                     /locus_tag="JQH22_003755"
     CDS             62..2494
                     /locus_tag="JQH22_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFB2207926.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2561..4030
                     /locus_tag="JQH22_003756"
     CDS             2561..4030
                     /locus_tag="JQH22_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFB2207927.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4030..5691
                     /locus_tag="JQH22_003757"
     CDS             4030..5691
                     /locus_tag="JQH22_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFB2207928.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5912..6253
                     /gene="symE"
                     /locus_tag="JQH22_003758"
     CDS             5912..6253
                     /gene="symE"
                     /locus_tag="JQH22_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFB2207929.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6300..>6617)
                     /locus_tag="JQH22_003759"
     CDS             complement(6300..>6617)
                     /locus_tag="JQH22_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207930.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1951 a         1580 c         1525 g         1561 t
ORIGIN      
        1 atagccaggc tattgatttt atgtacaatc agaatcgtcc tgataaaagc gagaaccccc
       61 catggcggaa ctgaacctaa gtaacctgac ggaagcagac atcattacca aatgcgttat
      121 gccagccatt ctcaatgcag gctgggacaa cacaacgcaa atcagacagg aggtcaaact
      181 ccgggacggt aaagtcatcg tacgtggtaa agttgcggca cgcagaacgg taaaatctgc
      241 tgacatcgtg ctgtaccaca agccaggcat tcccttagcc gtgattgaag caaaagccaa
      301 caaacatgaa attggcaaag ggatgcaaca gggcattgaa tatgcgcgcc tgctggacgt
      361 tccttttgtt tttgccacca acggtgatgg ctttatcttc cgcgatgcca ccgcagccga
      421 aggtgaatgc cttgaaaagc aaataacgct ggatgacttc ccctcccccg ctgaactctg
      481 gcaaaaattc tgcctctgga aaggatacac acaagctcag cttccggtga ttactcagga
      541 ctattacgac gatggcagcg gcaaatcgcc tcgttattat caacttcagg caatcaacaa
      601 aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg ctggtcatgg cgaccggaac
      661 agggaaaacc tataccgcat tccagatcat ctggcgcctg tggaaatcaa aaaataaaaa
      721 acgcattttg ttccttgccg atcgcaacat tctggtcgac caaaccaaaa ataatgattt
      781 ccagccattt ggtacggcaa tgaccaaagt cagcggacgc accattgatc ccgcttatga
      841 aattcacctc gcgctctatc aggctataac tggcccggag gaagaccaaa aagcgtttaa
      901 acaagtcgca ccagatttct tcgatctgat cgtgatcgac gaatgccatc gcggcagcgc
      961 atccgaagac agcgcctggc gagagatcct tgattatttc agttccgcca cccaaattgg
     1021 cttaaccgcc acgccaaaag agacgcatga agtctccagc acggattact tcggcgatcc
     1081 ggtttacgtc tactcactaa aagaagggat cgaagacggc ttcctcgctc cttataaagt
     1141 tgtccgtgtt gatattgatg tcgacctgca aggctggcgc ccaaccaaag ggcaaactga
     1201 cttaaacggc gaagtgattg acgatcgtat ctataaccag aaagatttcg atcgcacgat
     1261 ggtgatcgac gaacgcacag aactggttgc cagaaccatt accgactacc tcaagcgcac
     1321 caatccgatg gataaaacca tcgtcttctg taacgacatc gatcatgcag aacgtatgcg
     1381 ccgcgccctg gttaatctca acccgcagca ggtgaaaaag aacgacaaat acgtcatgaa
     1441 aatcaccggc gatgatgaaa ttggcaaagc tcagttggat aacttcatca acccgaaaaa
     1501 accgtacccg gttatcgcga ccacttcaga gctgatgacc actggtgtgg atgctaaaac
     1561 ctgcaaactg gtagtactag accagaacat ccagtcgatg accaaattca agcaaattat
     1621 cggtcgtggt acacgcatcg acgaacgtta cggcaaactc tggtttacca tcctcgactt
     1681 taaaaaagcc accgaactgt ttgccgatga gcgtttcgat ggcattcccg aaaaagtcat
     1741 ggataccaca ccagaggata tcgccgatcc agaatctgat tttgaagaga aactcgaaga
     1801 aatcagcgaa catgacgacg aacaggtaac aggcgttgat gaaccgcctg cgccaccata
     1861 ccaggttacc gataccgatg atgtcggccc atttccggaa gaagacgaga agaaaatccg
     1921 caagtttcac gtcaacggtg tagcggtggg cgttattgca cagcgcgttc agtattacga
     1981 cgccgacggt aaactggtta ccgaatcctt taaagattac acccgcaaaa cactgctcaa
     2041 agaatatgcc tcgctggatg actttacccg caagtggcag gacgccgatc gcaaagaagc
     2101 gatcattcac gagctggagc aacaggggat catctgggaa gtactggcag aagaagtcgg
     2161 taaagatctc gacccgttcg acatgctttg ccacgtagtg tatggtcagc cgccgttaac
     2221 ccgcaaagag cgcgccgaga acgtgcacaa gcggaactac ttcacaaaat actctgaagc
     2281 agcgcaagcc gtgctcgata atctgctgga taaatacgcc gatgcgggcg tacaggagat
     2341 cgaaagtatt caggtgctga aacttaaacc attcgacagc atgggcacct taccggagat
     2401 tattaaaacc ggatttggcg accgtaacgg gtataatcag gcgctcagcg agctggaaaa
     2461 cgaaatctac caattaccgc cccgctctgc ttaacaactc tcttttgcgc cttctgcaac
     2521 catcatgttg cagaaggcct tgtttttcat ggaatacaac atgtctatca gctcagtaat
     2581 caaatcatta caagatatta tgcgcaaaga tgccggtgtg gacggcgatg cgcagcgtct
     2641 cggtcagctc tcctggctgc tgtttttgaa aatcttcgat gcccaggaag aggcgctgga
     2701 actggagcag gataactatc aatatccgat cccacagcgt tatttgtggc gcagttgggc
     2761 cgccaacgcg cagggcatta ccggtgattc cctgctggaa ttcgttaatg atgatctgtt
     2821 tccggcgttg aaaaacctca ctgcgcctat cgataaaaac ccacgcggct acgtggtgaa
     2881 gcaggcgttc agcgatgcct ataactatat gaaaaacggt acgctactgc gccaggtgat
     2941 caacaagctg aacgaaattg actttaccag cgccagcgaa cgccatctgt ttggtgatat
     3001 ttacgaacag atccttaaag atctgcaatc tgcgggcaat gcgggcgaat tctatactcc
     3061 acgcgccgtc actcgcttta tggtggatcg cgttgatccg aaactcggcg aatccattat
     3121 ggacccggcc tgcggtacgg gcggttttct tgcctgcgcg ttcgatcatg taaagaacaa
     3181 atacgtgaag agcgtcgccg atcatcagac actgcaacaa cagatccacg gtgttgagaa
     3241 aaaacagctt ccgcacctgc tggcgaccac caatatgctg ctgcacggca ttgaagtacc
     3301 ggtacaaatt cgtcacgaca acaccctgaa taaaccgctt tcctcctggg atgagcaact
     3361 ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa gaagacggta ttgagaagaa
     3421 ctttccggca gagatgcaaa cccgcgaaac ggcggatctg tttctgcaac tgattgtgga
     3481 agtactggcg aaaaacggtc gtgcggcggt ggtattgcca gatggcacac tatttggcga
     3541 aggtgttaaa accaaaatca aaaagctact taccgaagag tgcaacctgc ataccatcgt
     3601 gcgtttaccg aatggtgtgt ttaatcccta taccggcatt aaaaccaacc tgttgttctt
     3661 taccaaaggt cagccaacca aagagatttg gttctatgaa catccgtatc cggcgggcgt
     3721 gaaaaactac agcaaaacca agccgatgaa gtttgaagag tttcaggcgg agatcgactg
     3781 gtggggtaac gaggccgatg gttttgccag ccgcgtcgag aatgagcaag cgtggaaagt
     3841 cagcattgat gacgtgattg cgcgcaactt caatctggat attaaaaacc cacatcaggc
     3901 ggaaaccgtc agccatgatc cggacgaact gttagcgcag tatgcaaaac agcaggcgga
     3961 gatccagacg ctgcgtaatc aactgcgcga tattcttggc gctgcgctgt ctggcaagga
     4021 ggttaactaa tgagtgtgga aaagctgatc gttgatcata tggaaacctg gacctcggcg
     4081 ttgcaaaccc gttccaccgc cgggcgcggc agctccggta agattgattt gtatggtatt
     4141 aagaaattgc gtgagctgat tctggaactg gcggtgcgcg gtaaactggt gccgcaggat
     4201 ccgaatgatg aaccggcgtc ggacctgctg aagcgtattg cggcggaaaa agcagagctg
     4261 gtgaagcaag gcaaaattaa aaagcaaaaa ccactgccag aaattagtga ggaagagaag
     4321 ccgtttgaat tgccggaggg atgggagtgg gtgcacctcc ctgatattta ttgctcaatt
     4381 tctgagtcat ctagaaaaat aaaatcctcc gaaatactac ctgagggaaa atatcctgtt
     4441 atcgagcaaa gccaagaatt tatttctggt tattgcaaca atgaatgtct gttaataaag
     4501 ttaaataatc cagttatagt ttttggtgat cacacaagga atattaaatt tattgatttt
     4561 gattttgttg tcggcgctga cggtgtgaag attttatcac ctatattaat ttgtgagcgt
     4621 ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc gagacatttt
     4681 aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga gcgaatagtt
     4741 gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc cctgaccagt
     4801 ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac
     4861 accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc
     4921 accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa
     4981 cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag
     5041 gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact gccgccaatt
     5101 agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg tattggtaat
     5161 atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt atatcaagta
     5221 gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac agtgaaagaa
     5281 tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta ttctaaaatc
     5341 cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag tgctgatatg
     5401 tatccattag actgttacat taatccaaat tacttattga aatatatcct ttccattcca
     5461 tttcttatgc aagtcaaaaa agcagagaac agaataaaaa tgccaaaact taattctgac
     5521 agtttttaca atattattgt tgcgattccc ccctataatg aacaacaagc aatatttgat
     5581 aagataaatt ctattgaagc agtatgcaat ggtcttattt cttatattgg gatttatcat
     5641 aaaacccaac tgcatcttgc agacgcactc actgacgcag cgataaacta attccctcgc
     5701 cctcacaaaa ccaataaagc ggctaaatag ccgctttatt cacatcacca aaatttatat
     5761 ttcacgctaa ttttcatctt attttcatta atttttagct ggactttccc tgtatttact
     5821 gatgatgtat ccaggtattt agcacggtgc ggatgtgcgc caacacactc gcaccgctaa
     5881 tcacaatcac tatttataga gaatagtaat catgactgac acgcattcta ttgcacaacc
     5941 gttagaagca gaagtctccc cggcaaataa tcgtcaatta accgtgagtt atgcgagtcg
     6001 ttacccggat tacagccgta ttcccgccat caccctgaaa ggtcagtggc tggaagccgc
     6061 cggttttgcc accggcacgg cggtagatgt caaagtgatg gaaggctgta ttgtcctcac
     6121 cgcccaaccc gccgccgccg aagagagcga actgatgcag tcactacgca aggtgtgtaa
     6181 gctgtcggcg cgtaaacaaa ggcaggtaca ggaatttatt ggggtaatta caggtaaaca
     6241 gaaagtcgcc tgatacattg acccggtagc gccagtgcta ccgggtatta ttcgccgcgt
     6301 tataccaacg cgctctccat ctgtttttca aatgcctgac gaattaatcc cataatgtaa
     6361 ggaagttgtg cgaggtcgct gaaaccaatt tctacatcgc cattacccca gcgcccaaca
     6421 tttgtcacat ctttggcaat acctttcgga tcgaccagtt catgaaactg catattaagc
     6481 gacaatcgca gtcggctttt ttgcggcaca acatcaacaa aattcgtttc cgctttaaac
     6541 gcaatatata gcttcaatac ttcctgcgtg atccctgcgt ccaggcgcat aacttcatcg
     6601 cgaagatgat cgaataa
//
LOCUS       DADLDT020000169         6586 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000169 DADLDT020000000
VERSION     DADLDT020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.173"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1282)
                     /locus_tag="JQH22_003760"
     CDS             complement(<1..1282)
                     /locus_tag="JQH22_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFB2207931.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQ"
     gene            complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JQH22_003761"
     CDS             complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JQH22_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HFB2207932.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JQH22_003762"
     CDS             complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JQH22_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFB2207933.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2251..2904)
                     /locus_tag="JQH22_003763"
     CDS             complement(2251..2904)
                     /locus_tag="JQH22_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HFB2207934.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3843..4700)
                     /gene="repA"
                     /locus_tag="JQH22_003764"
     CDS             complement(3843..4700)
                     /gene="repA"
                     /locus_tag="JQH22_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFB2207935.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4693..4767)
                     /gene="tap"
                     /locus_tag="JQH22_003765"
     CDS             complement(4693..4767)
                     /gene="tap"
                     /locus_tag="JQH22_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFB2207936.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(5012..5260)
                     /gene="repA"
                     /locus_tag="JQH22_003766"
     CDS             complement(5012..5260)
                     /gene="repA"
                     /locus_tag="JQH22_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFB2207937.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(5545..5694)
                     /locus_tag="JQH22_003767"
     CDS             complement(5545..5694)
                     /locus_tag="JQH22_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFB2207938.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(5941..6414)
                     /locus_tag="JQH22_003768"
     CDS             complement(5941..6414)
                     /locus_tag="JQH22_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207939.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
BASE COUNT         1719 a         1604 c         1453 g         1810 t
ORIGIN      
        1 cctgtttaaa atcataattt ggttgttctg ttcttgagtt attaagtaga gttcttatca
       61 gtgtttgcca agcatgattg ataaatttat tttctgattc tttaaaatat cgtctgatta
      121 aagcacataa atcttcgaca cttttctgac gagcgctagc tgcccaacgg cccccttcgg
      181 tttgcaccat tgaacgttgt acactgtctc ctaactgagc gataaatttc tgttcatcgt
      241 caagttgcat gttttcattt atgattaatt catacaaagc aagaaaaaca gcttgatagt
      301 aacgaggaca ttcttgagat gaagatgcat tgcccagaat gtgtgatcta aagttcaaat
      361 tatttgcatc aaataatttt tgaatcatct cataaacgaa gaggtaatta ttgacaattt
      421 tatctgcacc atttgtctga atgtaattct ctaattgagt tcgtgtatgg attgcatgac
      481 tcgtcgaacc ttcaccgtaa aatgtatcga gcgatgttga tcctgatgtt ggcttatcat
      541 ctaaaaatat atagcccaag atatttgcaa ctaactcttc atcgtcagag tctctaatgc
      601 taggcctact aatgatatga tttctaatat agaaatgatt gtctatattt attccataat
      661 cgagtccatc attgcaaatt gatatgttat gtattttatt cagtcccatt atatcgctat
      721 gtgtagtatc tcctcttata attgtcgata ttttccttac taactctgaa aaattactaa
      781 cgcaccccgc acttcgtagt tcttgttttg ataaaatttt accattggaa tttatgcgtc
      841 gaaacacttc gtcaatgtgg tcctctgttg aagagtattc agatatagca aatctatagc
      901 gagcaattaa tgtagaactt tcgcgggata gcactggctc tttttgttga agtttaccac
      961 tgtcttttag ttgttttgtt aatgcaatgg catctaaatt aaagtattta ccatttaatg
     1021 aaaatcgctg ctcaataaaa ctcattaaag cttctagtct ttgcattcca tcaaggattt
     1081 ctcttttgtt gtcaatggaa acaaacaata gcaaagggat agggtattgc tgagtcattg
     1141 atgaaatcag tgctgttttc tcttcgaggg accatactaa ttttctttgg tatcttcgat
     1201 ttataattaa tttatcatcc agataccact cgtataattc ttctgctgtc ttggagtcaa
     1261 tatctaagtt ttcattagcc attagatgta tctcgagtat ttagttaaga aactgatagg
     1321 tcagtatttg catcaatgtt cattgaattt gtatccgaag tcaatctgat gtaggaactt
     1381 caaaactgcg caaaacacgc aaatacgcac aagaaagtag ggctgcgctt ggaattcgga
     1441 aaataaacca catcaaaact actttttagc caaataacgt ttgggaatca ccagaatggt
     1501 gggacaacag cggttttagt gccctaaatc gtacgttttc atccagttgc ccctcaaacc
     1561 ccatgttcaa gtcagaatag tggacaggcg gccaagaact tcgttcatga tagtctccgg
     1621 aacccgttcg agtcgttttc cgccccgtgc tttcatatca attgtccggg gttgatcgca
     1681 acgtacaaca cctgtggtac gtatgccaac accatccaac gacaccgcaa agccggcagt
     1741 gcgggcaaaa ttgcctccgc tggttacggg cacaacaaca ggcaggcggg tcacgcgatt
     1801 aaaggccgcc ggtgtgacaa tcagcaccgg ccgcgttccc tgctgctcat gacctgcggt
     1861 aggatcaagc gagacaagcc agatttcccc tctttccatg tcagatttcc tcctgaccag
     1921 tcgccggtgc atccagccat tctcgttctt cagctgatat ttcagcattc ggatcacact
     1981 gtgccagtag ctcagccagt gaatattgcg ggcgtctgta cggctcaaca atcagccggc
     2041 cattatcaat gaccatgcca acttcattat ctgtgcccag agacagcgca ttcagcagtg
     2101 ccggtgggac ggtcagcata actgagccgc caaccctctt cagtcgggtg gtatgcattc
     2161 ttcacctcca taaaagttat atttaaatat aacatccact aaaaaaacac accaggcttt
     2221 aacgcacaat gtttaataaa aatataactt tcaaccaaac agtaaaccca gcgtggttgc
     2281 ttccatatgc agcaataccg gcagcagcag gccaccactt ctgatcctgg ccactgatgt
     2341 aatcaacccc accaggaaca gttctgccag tgtcagcagg ttctgatact ggctgtgcgc
     2401 ggcgacgaac aacaacgacg ttatcagcgc ccccagccac atcgtccagc agtaccgtga
     2461 acggaagacg ttcagcataa tcccccggaa cagcgtttcc tcattcaacg gggcaaggat
     2521 aaagatggtc agcaacgtca ggatcacgtc aggtatggac ttatcggcaa aaagtttcgt
     2581 cataaatggc tcagcaggca gagccagcgc cttaccgagc agaaatacac cgacatacac
     2641 cacggccatc gcaccgacca gccacggtac gccaacgttg cgcagctgac caacgaccgg
     2701 tagcggagct atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt
     2761 accatgactg aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat
     2821 gacaaatctg gctccccaac taatgcccca ggcagccagc ataaccagca taaactgcag
     2881 atattgatta cgtgtttgaa tcattgcatc gcctgtaaat ttttaacttg tcctattttt
     2941 gtcattacca cgtatataca catgtataac aattcagata tcgttaccag gatatgccgc
     3001 atcagcggca tggaaggcgg cactctgttg tttcatatga tacaggagta aaaccgccga
     3061 agcccggcgt aagccggtac tgattgatag atttcacctt acccatcccc agccctgcca
     3121 gaccataccc gctttcagcc atgagagagc ttctgtgcgc ggtcggagtg gtcccgacga
     3181 gggtttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
     3241 cgtgtactga gaacttccag cgagaagact gacagcgatg aagatgtagt tacaacattc
     3301 ataattaaaa gcgactctgt tccggccctt tgggccgggg cggggccgct tttcagttat
     3361 gagggagggg ctttgtggtt tcggttctgc gctggaccgg ggtttttctg gaggttgttt
     3421 ttgtgtgttg taactaaagt ggctccggtc ggggcccgcc gcttgcggtg ggaggtgcat
     3481 atctgtctgt ccacaggaca ggcagtgaat aggttttctt tttaaatgaa tgtaattaag
     3541 tagtttaaag gagatataaa caggtgttta aaagatacat tgcaccctgt aagactggcg
     3601 gctggcgctt tatgacatga acggttgtaa ccttatgggg aagtcccttg cagttaaatg
     3661 tggataagca aaattccccg tcgctgaggc gtattttgta ttaaaaacag ggggaatcgg
     3721 atgctccaga aggtggatga tgagattgtt ttttgcatgc gacgctgttt ttttgtgcac
     3781 cggcgggctt caggcgtgcg aatgcctccg gcgcgggccg gattattcag aggaggtcac
     3841 tttcaggggg aagctgtggc cagccggctg taattacggt tacgtgacag aatcatgcgc
     3901 tccttcacac gacgctcaac ttcgcgtttt accgcctcac gattggcagt gaagcgccct
     3961 tccgcgattt cgcgcgtcag ctgccgtttc accagggtga caatatcctg acgttccctg
     4021 tccgcatcac gacgcgcacg ggcacgcttt attccccggg acttaagctc tgtctgataa
     4081 ctgcgaaaac gctcacgaac aaaacgccag gctttcgcta tcagttcatc catgcccagg
     4141 gtatccagcc cctgcttttt gcgttgtttg ttttcccata ccacacggct gcggcgcgcg
     4201 gcggccgctg cctcctctga tacatcgagg gcagcaaaca gtgcagatgt gaacgtgata
     4261 tcggtcggaa tgtagcaccc gataagcggg tcatattccg tctggtaggt aatcagtccc
     4321 agctctgaca ggaacgtcag ggcccgggtg gcacgggtga tggagagttt tccggcagca
     4381 gactccgtcg ccagtccgca ctcaatggcc agcgtggtga tggagcactg gacgcggttg
     4441 gccagcgggt catagtggaa acacagcccc tgcaggagcg catcaatagc ccgtcgacgc
     4501 agcaccggtg gcatgcgtcg acgcagacca cgcgaacggg catgcgccac atgaatggcg
     4561 aaatcaaaac gggaagtgaa gcccaccgcc ttttccatca gtttttcgca gaacttcagc
     4621 gttccggcac ctttacgggg tgtaaacacc ggattcgggt tctttacctg gcggtaatac
     4681 gtttgttgaa gatcagtcac accatcctgc acttacaatg cgcagaagga gtgagcacag
     4741 aaagaagtct tgaacttttc cgggcatata actatactcc ccgcatagct gaattgttgg
     4801 ctatacggtt tttagatggc cccggtaatc ttctcagtcg ccaaacttgt cgaagattat
     4861 cggggttttc acttttctgg ctcctgtaaa tccacatcag aaccagttcc ctgccacctt
     4921 acggcgtggc cagccacaaa attccttaaa cgatcagcaa tctatcacgc acaccagaaa
     4981 caggcaagaa tgtgatgtcg ctacatttta gttagctaaa agcactcttc tgctttatca
     5041 gttcggcaat catctcagcc tgggtaacgc cctcagcttc acacatcacc tgcaattgcg
     5101 cttttaacgc agcaggaatg aacactctga gttctttatg tgtagctcgt tttcttgcca
     5161 gggatgcctg ctgacgttct gcaagcgtca gaggattacc ttttctgtat gctcgctttg
     5221 tacctgatga ggaagtcact gcattttctg tctgcgacat ctcgcctcct caatactcaa
     5281 acagggatcg tttcgcagag gatactacag ttttttgaaa tcagcgactt gagaattgtg
     5341 acgaagatct gtgttggcgc acaaatcaac ggggattact gttgtttaat gtgatttttg
     5401 ttgcgaaatg gctgaaaaaa caggaaatgc cttaacccgg gagccggccc cgggcgggac
     5461 cggctgtttc tggcatgtcg ggtgtctgcc cctgaaatac ctcacacttc cggaaagcgg
     5521 ggacttccgt ccccgcccgg cagtttactt ccgtgcttcg taggccagag ttacctgcac
     5581 cactgtattt cccctgtgaa tattcagctc acataaccgt tccctgaata tcagtgaaaa
     5641 acacaacacc gtggcgcaca cggcgagcaa cccgataagg gcatacttcg tcataaactt
     5701 tgacctctct gcaaggaaga ggctacaatc agtagtgcaa tgtacttcat gcagacctca
     5761 cgagttaatg gattaacaag tggggtcttt gcatttctgg tacctgcgtc ttgcctgaac
     5821 cagaaaggct cagacacccg ccgcgataat accacattcc ctgttgctga ctattacctg
     5881 cattgcccgc tttttactac agaagcacga aggcgtcact ccagccaata agtatgcttt
     5941 ttatttacat ccatttttaa tccggtaccg acgttttcct ccagttaact cgtttttcgt
     6001 atgactcatg gcaataaaat tttctttacc atatgacaac atcccaataa tatcaaagtt
     6061 gtcttttaat ttgatggcat ccttttcact tacagcgaca ataagttgtt taccaaattt
     6121 tctgaccatc attgcaaact gtgatactgc tgatccacca cctaaatcag gccacccagc
     6181 acaattaaaa atgattgtct gctcttcact attcattgca tccctgataa aggagacagc
     6241 atcctcattt ctctctacat ctgaatcact gttaatctga gtgaaatcat aatatttaga
     6301 acctggtata gaatacccct taaaaaactt atcaatgtgg gaggcagagt tcccaacaat
     6361 aagaatattg tgttcacgct taatcatcaa ttctgataca tcatcatatt tcatatattc
     6421 accattatta tataaagttt gggtacaggt ggttccggaa tttactctgt gaccaccatt
     6481 tcccgctttt tattacagaa acgccgggag cggggaaaac ttgctttttc ccgtttccgg
     6541 ggttggacaa ctgagcaacg cgaaggcgtc agctacgatg ttccgg
//
LOCUS       DADLDT020000170         6439 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000170 DADLDT020000000
VERSION     DADLDT020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.174"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..117
                     /locus_tag="JQH22_003769"
     CDS             <1..117
                     /locus_tag="JQH22_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFB2207940.1"
                     /translation="MQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(456..1226)
                     /locus_tag="JQH22_003770"
     CDS             complement(456..1226)
                     /locus_tag="JQH22_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207941.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1271..1705)
                     /locus_tag="JQH22_003771"
                     /pseudo
     CDS             complement(1271..1705)
                     /locus_tag="JQH22_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1717..1938)
                     /locus_tag="JQH22_003772"
     CDS             complement(1717..1938)
                     /locus_tag="JQH22_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207942.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1939..2622)
                     /locus_tag="JQH22_003773"
     CDS             complement(1939..2622)
                     /locus_tag="JQH22_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFB2207943.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2686..2997)
                     /locus_tag="JQH22_003774"
     CDS             complement(2686..2997)
                     /locus_tag="JQH22_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207944.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(2994..3896)
                     /locus_tag="JQH22_003775"
     CDS             complement(2994..3896)
                     /locus_tag="JQH22_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFB2207945.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4336..5295
                     /locus_tag="JQH22_003776"
     CDS             4336..5295
                     /locus_tag="JQH22_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFB2207946.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5295..5690
                     /locus_tag="JQH22_003777"
     CDS             5295..5690
                     /locus_tag="JQH22_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFB2207947.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5873..6253
                     /locus_tag="JQH22_003778"
     CDS             5873..6253
                     /locus_tag="JQH22_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFB2207948.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQHITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6299..>6439
                     /locus_tag="JQH22_003779"
     CDS             6299..>6439
                     /locus_tag="JQH22_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2207949.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCS"
BASE COUNT         1755 a         1684 c         1556 g         1444 t
ORIGIN      
        1 atgcagcttg aacgccagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttgc ctgcgctctg gcaaataaac tggcccgaat tgcctaggca
      121 atcactactc agcaaaaaga atatcaggcc tgatatatag atgctgacaa aactcaatta
      181 gcttattgca aacaataaga tacaagttac cgtctggttt tgcgaagatt gattattgat
      241 gacatgaacg gcaaatcgac ctgattaaaa acctaacagg ggaaaatggc tcactgaagc
      301 cgaggggatt tttaggatta tcaggtgcgg aactcatcga ggcgcgggca tcccttgccc
      361 acagagacgc cggatagatt taagcaagcc caccattaat caaaaatcag tgttgcaaaa
      421 acgggggtga ccatagattt tccccgccat cgtcatcatt tccggatttt tgccagggca
      481 tccgccatgc tgcggatttc ggcccgggct tcccggacaa cttcccgcag aacaccacgg
      541 tcaaaccacc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
      601 gacaggatat gctcaataca atcgctgacc agatacattt ccaccgccga aaaacgtgcg
      661 ccccggttca ggatggcatc gagcaccacg ggcaggtttt catcgtcttc atcgagccag
      721 caggggtgat cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagcaac
      781 gtctgaccgc ttgtggtcag cgccgcccgg atacgccgcg catcttctgc cccccggcaa
      841 ctgacactgc atactgcatc gcgcaccgca tcaagaactg ccctgatttc gtcttcggtc
      901 agccattcac cggcatacgg gaaagacacc tgcgttccgt ccgccggaag taccggcagc
      961 gactgccact gactgccgga cagaacaagt tccgctccgg ttccggtcag tttctgaatg
     1021 gcggaggcca ggtgcggcgg cagcacgcca tcgtggcggt taacgagtaa cgtcgccagc
     1081 cggggcgcat ccagccgcac gtaaatgctg atccagcccg gatagttcgg gttacattgg
     1141 tcaacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtgacgacaa
     1201 atcagagcat tcagcgtttc attcataatt catgaactcc ctgtatcgcc gcccacaggc
     1261 ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag caggctgcgg gcctgatcaa
     1321 ccatcgcctg tcggcgggta acgtattccg ggagggggaa accgtgatcc cgggcagccc
     1381 attcacgttt ccacagtgcc tgttcagcga tctgagtcag ggtctccagc agttcagcag
     1441 ggacggtcac ctggcggttt tcgcgcacgt tgctgataat ctccatcgcc gcggcggaag
     1501 aaataccgga ctcaatccgc tagtcttccg ggatgtcctc caggtgcgcc aggacggtcc
     1561 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctga atctcttccg
     1621 gggaccagat aaccagcatc agcggcgtgt ctcccgaata ctttgctgcc agcgcccggc
     1681 atagcgtttc gcatgttccg tacatactct ctcccctcaa tctttgaagc aggaatccag
     1741 ttccagaatt tcggtatatg cctccatcaa gaggcggttt gcgcgaaaca acgtctgacg
     1801 caccagacgt tccggcaggg tgtcagcatt aaagctgtac cgccgctcca tctcattcat
     1861 ggttgtacgc aggtcgcaca tggcattggc cacctccgcc acttcggcgc gaagtttttc
     1921 gtgtccggca tagttcattt acgcagcctc cggcataaac cagtcatcat tcgcggcccc
     1981 ctgctgcatg gcccgctgta cggcagccag tcgttgctgt ccggcacggt gatactgctc
     2041 aagcagctcg ataccgatat accggcgtcc ggactggagg gcggcgacgc aggttgaacc
     2101 gctgcctgca aacgggtcca gcactatcgc attcgggtgt gtgaagctct caatcagcgg
     2161 ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg ttgcccgaat atttccagcc
     2221 caacacgtcc ggcagcgggt tttgtggcag acgtggacgg ccttttgcca ggatgtaggc
     2281 gcattcgtgg cgatagccca catatgcggc cttcgatgtg tagtttttgg tgaagaccag
     2341 gtgaccaaca acgctgaatc ccgcattttt ccaggcagac ataaagcgat cgacgcggtt
     2401 ccagccatag aagctcacca tcagcgcgtc ttttttgagt acgcggtaca tttcattaca
     2461 ggccggttgc agccattcat cagttttatc accagcaatg gtacgcccct gacggtcacg
     2521 aaaaccgacg agatatggcg gatcggtgag gatgaaatca atggcgttat caggaatacg
     2581 ggccataaca tcgatgcagt taccgaggac aaaacgggac ataacagatc cttctcttac
     2641 agagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcatc gcttccggta
     2701 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tttgacagca aggttatgcc
     2761 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac gggctggcgt acactgtcac
     2821 cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc cgccggacgc gggacactga
     2881 ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag gagcacggcg
     2941 acatcctttg tcggaaaaga aacgggcggt tgcccgcccg tctccgtcat acttcatccg
     3001 ccataatgcg ccacattgtc gactatccac gcaggtccgg tgacttccgg ctgctcatcg
     3061 tcatctgtcg gggaagacca ttcaagttcc ccccacagca catcgacgag ctctccacgc
     3121 tcatacaact gccagccaca gaaatcacag ccctgttcgg cataccagtg ctccagcgtg
     3181 cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt ccggctgaca ccacggagtg
     3241 tcgaaatcca cctgaatgaa gttttctccc tgactgctga tattcaggtc atatccacaa
     3301 ggccacttca cgccataccg ttccgtatac cagtgatatg cagaaggaac accgttcaga
     3361 acaccaccat taaagccgtt cacttcaaca tccaggcggg tcggcagaac cgtcagcagg
     3421 tcaaacggca tggttttttc cggcactacg ttgtcacaca gacggttcca ccacaccgat
     3481 acatcctcgt taccccatat gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg
     3541 atggtttccc tggcatcatc cggtaatcct tcccaacgac gctggccagt accgctctgt
     3601 aaccagagtt catgcagcat caggcagttt tgttcatcca gcaacacacc gttctgcagg
     3661 tgtgtcagcc agcgggtgaa cgcgatattt tccgtcgata cagcaccacg tccggcagcc
     3721 gtcactccgg ggcactgttc agagcggata ttttcagtga tctgcagaag tccggcactt
     3781 ccggccagaa acagctgaat accttcattt gtggcgcggc gataaagcgg tgtgactgca
     3841 ccgctggcca gtcgtttaat ctcagcgatc tgtgccggtt caccaggaaa atacatacga
     3901 ttcgagcacc agtttggcat gggaattctc ctctcacatg cggcctccat caggccgtgc
     3961 cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt
     4021 ggaggaggaa tgacgaaacg tgaccgggtg aggaggccgg cgacagcgac acacttgcat
     4081 cggatgcagc ccggttaacg tgccggcacg gcctggttaa atagtatccg tctcagttcg
     4141 tggcgcaaaa tgagaaacca ataagaactc aaaatgaacc acaacaagaa cataaagaga
     4201 ctctaaaaga gcaacaccgt ttgacataga ctcaaaatga gtcacaataa gaaacaataa
     4261 aaaactcaaa aagatgcacc gacataaagg atgctcaatt tgagataaac acaaactcaa
     4321 aacgaggcaa aaagaatgct aaaagtatca tgcgatgatg gttcaactaa cgtaaaactg
     4381 gcctggcttg aagatggcga ggtaagaacc agtctgtcag gaaacagctt taaagaaggc
     4441 tggaacccgg gactgtttaa tgccggcaaa gtttacaatt acgtcgtgga tgaaaaaaaa
     4501 tacacctatg atttgggaag cactgctgtc ataggtacaa cacatgtcag ttatcaatac
     4561 agcaccacca atctgcttgc catacatcat gcactgctga catccggcct tcagccacag
     4621 gatgtagaac tgacagtaac attacctgtg accgaattct ttgataatga caatcaacca
     4681 aatgaagaaa gaatagaaag aaaaaaggca aatgttctcc gtgagatatc tctgaataaa
     4741 ggggaaacat ttaaaattaa aaaagttaat gtaatgcctg aatctttgcc agctgcattt
     4801 gaatcactaa aaaaagataa agtaaacaaa cttgaacgca gcctgattat cgatcttgga
     4861 ggaaccactc tggattgtgg tttaattctc ggtgcatttg aaggcatttc tgaaatccgt
     4921 ggctattcag aaatcgggac atcccgcatc actcataccg tcatgaatgc gctgacgaaa
     4981 gcctccacac catgtaatta ttttattgct gatgagttaa taaaaaacag acacgataat
     5041 gaatatctcc agacattaat aaatgatgta gctgaaataa aaaatatttc tcatgtaata
     5101 gacagagaag tgaaatctct ggcagaaagc atcaggcagg aaatttcaac attctcagga
     5161 atgaaccgaa tctatctcac tggcggagga gccgagctta tttaccctca cataaagcaa
     5221 tatttcccga atctgaaagt gaacaaggtg gatgaacctc agtttgcatt ggtgaaagca
     5281 atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag aaagaaagtt gtggcatacc
     5341 tgcacccggc actttatccg caggacaacc tgacacaaca aacaatagac tctctcccag
     5401 tacaaatgcg gggagatttc tacagacaat cccttatttg cggagcagct ctttattctg
     5461 ttgccccccg actgttaacg ctaatcagcg tttttttcag tgagaaaatc actgcagaaa
     5521 atctggtgaa acttatagaa caaacaacag gatacacatc cacatcaatt gatataagtg
     5581 tgttaaaaaa catcatagag gcatcttcag aaaacaaatc agagagcatc acttcaaaag
     5641 atgatttcga agaacaaacc agacgcaatc tttctatgtt aaaaaaataa taaaagaaga
     5701 gcaggttcag tcaattacct gctcttcttt tatacatact aaaaaatcag atttttacga
     5761 atattcagat tatcataaaa aatgcaacag acaggaatgc aataattttg cattatctcg
     5821 ttgcttgtaa tgcaaatatg cattattgtc agtgtgtgac aataacagag agatgacgat
     5881 ggcgaccatt aacgcacgta ttgatgatga tataaagaac caagcagatg aagtgctgaa
     5941 gctgatgaat atcagtcaaa ctcaggctat tgcagctttt tatcagcata ttactgaaca
     6001 aaagaaactg ccatttgtga tcacctcaat tgtaaaaacg ccgcatgatt tattacgtga
     6061 aagtacagac atgttagctg aagctcttgc agtgatttcc aatctacagg tgtggactga
     6121 acagcaggat gggattggaa aagcaaagct aatggagtat taccgccgcc tcgacgcact
     6181 gtactgttgt gcaaaagaga aaataggtct tctttcagat aaccgggatg ctgaactagg
     6241 ctgtgtccct taatcatctg agttatagta actgtctgtt tacacaacat attgaactat
     6301 ggctcgctac gaccttcccg atgaggcatg gaccatcatc aagcccctgt tacctcctga
     6361 acccgccaca ccacgggccg gacgcccatg ggctgagcac cgtaaaatca tcaatggcat
     6421 gttctgggtg ttatgttcc
//
LOCUS       DADLDT020000171         6405 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000171 DADLDT020000000
VERSION     DADLDT020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.175"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQH22_003780"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQH22_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFB2207950.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="JQH22_003781"
     CDS             complement(1433..1759)
                     /locus_tag="JQH22_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFB2207951.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="JQH22_003782"
     CDS             1894..2235
                     /locus_tag="JQH22_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFB2207952.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="JQH22_003783"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="JQH22_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="JQH22_003784"
     CDS             complement(2833..3543)
                     /locus_tag="JQH22_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFB2207953.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="JQH22_003785"
     CDS             3651..3956
                     /locus_tag="JQH22_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFB2207954.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="JQH22_003786"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="JQH22_003786"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="JQH22_003787"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="JQH22_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1707 a         1628 c         1502 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tggga
//
LOCUS       DADLDT020000172         6374 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000172 DADLDT020000000
VERSION     DADLDT020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.176"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="JQH22_003788"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="JQH22_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="JQH22_003789"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="JQH22_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="JQH22_003790"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="JQH22_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFB2207955.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="JQH22_003791"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="JQH22_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HFB2207956.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="JQH22_003792"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="JQH22_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFB2207957.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="JQH22_003793"
     CDS             complement(5701..>6374)
                     /locus_tag="JQH22_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HFB2207958.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1534 a         1680 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DADLDT020000173         6354 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000173 DADLDT020000000
VERSION     DADLDT020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.177"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..370
                     /locus_tag="JQH22_003794"
     CDS             <1..370
                     /locus_tag="JQH22_003794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207959.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="JQH22_003795"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="JQH22_003795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFB2207960.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="JQH22_003796"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="JQH22_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFB2207961.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="JQH22_003797"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="JQH22_003797"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFB2207962.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="JQH22_003798"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="JQH22_003798"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFB2207963.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="JQH22_003799"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="JQH22_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFB2207964.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..>6354)
                     /locus_tag="JQH22_003800"
     CDS             complement(5095..>6354)
                     /locus_tag="JQH22_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HFB2207965.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1439 a         1837 c         1525 g         1553 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaggaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagt
//
LOCUS       DADLDT020000174         6335 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000174 DADLDT020000000
VERSION     DADLDT020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.178"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            254..1642
                     /gene="narU"
                     /locus_tag="JQH22_003801"
     CDS             254..1642
                     /gene="narU"
                     /locus_tag="JQH22_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFB2207966.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1724..5464
                     /gene="narZ"
                     /locus_tag="JQH22_003802"
                     /pseudo
     CDS             1724..5464
                     /gene="narZ"
                     /locus_tag="JQH22_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5461..>6335
                     /gene="narH"
                     /locus_tag="JQH22_003803"
     CDS             5461..>6335
                     /gene="narH"
                     /locus_tag="JQH22_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFB2207967.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1492 a         1685 c         1663 g         1495 t
ORIGIN      
        1 attcacgatc tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg
       61 ttagcttgtt agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc
      121 gcggttacgc tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc
      181 taataactgg ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca
      241 tatgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg
      301 gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct
      361 ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt
      421 taccgttaat ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac
      481 cgcattaccc tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat
      541 attcggtgga cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg
      601 gctcggaatt gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt
      661 gctatgcggt tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt
      721 tccaaaagcc aaacaaggga gcgctcttgg gattaatggc ggattaggaa acttaggtgt
      781 aagtgtaatg cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg
      841 cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat
      901 ttgggtaccg ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc
      961 cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct
     1021 gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc
     1081 catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc
     1141 atttattggt gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt
     1201 gcgggtgacg ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac
     1261 cttaccgggc acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt
     1321 tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca
     1381 gataaccatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc
     1441 cgtcaccgaa acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt
     1501 ttttattccg caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat
     1561 gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg
     1621 gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac
     1681 aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga
     1741 tcgctttcgc tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat
     1801 gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt
     1861 gcgttccacc cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa
     1921 tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc
     1981 caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc
     2041 taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct
     2101 caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg
     2161 cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact
     2221 aaaccagctg attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt
     2281 tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta
     2341 tctgtcgctg cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc
     2401 cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa
     2461 ctccagctac atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca
     2521 cttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc
     2581 tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct
     2641 ggcgatggca atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta
     2701 ctttatcaac tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg
     2761 cgacgatggt agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact
     2821 gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt
     2881 agtgccgaac ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc
     2941 cattgccgcc ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt
     3001 tgcaggcgtg gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa
     3061 acacaatccg gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa
     3121 cacctgtccg gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg
     3181 gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg
     3241 gggtgagcaa attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc
     3301 cgataccgcc cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca
     3361 ctggtatcac atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg
     3421 tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca
     3481 aaccggctgg ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa
     3541 cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca
     3601 ggagttactt tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa
     3661 cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct
     3721 cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc
     3781 gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc
     3841 gcgtaatttg tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta
     3901 tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag
     3961 cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga
     4021 cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct
     4081 gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat
     4141 tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta
     4201 caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga
     4261 cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt
     4321 gctcgactgg cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt
     4381 ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat
     4441 ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt
     4501 cctcggcaaa ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga
     4561 caccgccatt gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt
     4621 tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc
     4681 gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat
     4741 tatctccagc cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata
     4801 caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca
     4861 ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga
     4921 cacccgtagc gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact
     4981 taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct
     5041 aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga
     5101 actgaccatt gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc
     5161 ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga
     5221 acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc
     5281 ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg
     5341 gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa
     5401 gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
     5461 atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat
     5521 acctgttcgg tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg
     5581 tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa
     5641 gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc
     5701 aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat
     5761 tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat
     5821 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     5881 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5941 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6001 ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct
     6061 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6121 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6181 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6241 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6301 gaggaagcgt cgagcaccga gcgcgaagtt gacct
//
LOCUS       DADLDT020000175         6335 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000175 DADLDT020000000
VERSION     DADLDT020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.179"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="JQH22_003804"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="JQH22_003804"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFB2207968.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="JQH22_003805"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="JQH22_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFB2207969.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="JQH22_003806"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="JQH22_003806"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFB2207970.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="JQH22_003807"
     CDS             4130..4885
                     /locus_tag="JQH22_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFB2207971.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="JQH22_003808"
     tRNA            4964..5037
                     /locus_tag="JQH22_003808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6335
                     /locus_tag="JQH22_003809"
     CDS             5152..>6335
                     /locus_tag="JQH22_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFB2207972.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DS"
BASE COUNT         1584 a         1586 c         1639 g         1526 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtag
//
LOCUS       DADLDT020000176         6331 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000176 DADLDT020000000
VERSION     DADLDT020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.180"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            5..436
                     /locus_tag="JQH22_003810"
     CDS             5..436
                     /locus_tag="JQH22_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFB2207973.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="JQH22_003811"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="JQH22_003811"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFB2207974.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="JQH22_003812"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="JQH22_003812"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFB2207975.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="JQH22_003813"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="JQH22_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFB2207976.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="JQH22_003814"
     CDS             complement(4438..5145)
                     /locus_tag="JQH22_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFB2207977.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="JQH22_003815"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="JQH22_003815"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFB2207978.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="JQH22_003816"
     CDS             complement(6003..6206)
                     /locus_tag="JQH22_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFB2207979.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1405 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct t
//
LOCUS       DADLDT020000177         6268 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000177 DADLDT020000000
VERSION     DADLDT020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.181"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..666)
                     /locus_tag="JQH22_003817"
     CDS             complement(<1..666)
                     /locus_tag="JQH22_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HFB2207980.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="JQH22_003818"
     CDS             complement(656..2413)
                     /locus_tag="JQH22_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFB2207981.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="JQH22_003819"
     CDS             complement(2429..3313)
                     /locus_tag="JQH22_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFB2207982.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="JQH22_003820"
     CDS             complement(3324..3929)
                     /locus_tag="JQH22_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFB2207983.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6268)
                     /locus_tag="JQH22_003821"
     CDS             complement(4100..>6268)
                     /locus_tag="JQH22_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFB2207984.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1835 a         1568 c         1316 g         1549 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgttt
//
LOCUS       DADLDT020000178         6254 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000178 DADLDT020000000
VERSION     DADLDT020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.182"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JQH22_003822"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JQH22_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFB2207985.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JQH22_003823"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JQH22_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFB2207986.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="JQH22_003824"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="JQH22_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="JQH22_003825"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="JQH22_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1456 c         1477 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DADLDT020000179         6241 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000179 DADLDT020000000
VERSION     DADLDT020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.183"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..361)
                     /locus_tag="JQH22_003826"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="JQH22_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="JQH22_003827"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="JQH22_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="JQH22_003828"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="JQH22_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="JQH22_003829"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="JQH22_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFB2207987.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="JQH22_003830"
     CDS             complement(2629..2943)
                     /locus_tag="JQH22_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207988.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="JQH22_003831"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="JQH22_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="JQH22_003832"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="JQH22_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFB2207989.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="JQH22_003833"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="JQH22_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="JQH22_003834"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="JQH22_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5609..5768)
                     /locus_tag="JQH22_003835"
                     /pseudo
     CDS             complement(5609..5768)
                     /locus_tag="JQH22_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5772..6241)
                     /locus_tag="JQH22_003836"
                     /pseudo
     CDS             complement(5772..6241)
                     /locus_tag="JQH22_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1672 a         1388 c         1274 g         1907 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      181 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      241 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      301 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
      361 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gagcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3001 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3241 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataacctt tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tgtccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacaaat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagca
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt
     5401 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     5461 aattacacca taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc
     5521 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     5581 cccacataat atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga
     5641 gaccaccaca ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca
     5701 ctgttgcccg cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc
     5761 agcgaatgaa ttttcatctg ttgctgtatc cagtcataca gtgactgcat cagtggcgcg
     5821 gctctggctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5881 atggcataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5941 gcatgcacat cgtggatttt tctccggaca tgagccatac atgcggcttc cgttattctg
     6001 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     6061 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     6121 gccggaggca tttccgaacc ggcgttacgg tcatcacgaa cgtagaccca cagccgggca
     6181 gtccttgttt taccgctgcc cggctcctgg accggaacgg ggatatcatc ggcatggact
     6241 t
//
LOCUS       DADLDT020000180         6161 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000180 DADLDT020000000
VERSION     DADLDT020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.184"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..430
                     /locus_tag="JQH22_003837"
     CDS             <1..430
                     /locus_tag="JQH22_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207990.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="JQH22_003838"
     CDS             550..723
                     /locus_tag="JQH22_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFB2207991.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="JQH22_003839"
     CDS             823..1644
                     /locus_tag="JQH22_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFB2207992.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="JQH22_003840"
     CDS             1986..2459
                     /locus_tag="JQH22_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFB2207993.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2475..2951
                     /locus_tag="JQH22_003841"
     CDS             2475..2951
                     /locus_tag="JQH22_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFB2207994.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3014..3235
                     /locus_tag="JQH22_003842"
     CDS             3014..3235
                     /locus_tag="JQH22_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFB2207995.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3398..3772
                     /locus_tag="JQH22_003843"
     CDS             3398..3772
                     /locus_tag="JQH22_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFB2207996.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3819..4193
                     /locus_tag="JQH22_003844"
     CDS             3819..4193
                     /locus_tag="JQH22_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFB2207997.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4190..4681
                     /locus_tag="JQH22_003845"
     CDS             4190..4681
                     /locus_tag="JQH22_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207998.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQISAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            4693..4890
                     /locus_tag="JQH22_003846"
     CDS             4693..4890
                     /locus_tag="JQH22_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2207999.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4975..5535
                     /locus_tag="JQH22_003847"
                     /pseudo
     CDS             4975..5535
                     /locus_tag="JQH22_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            5607..>6161
                     /locus_tag="JQH22_003848"
     CDS             5607..>6161
                     /locus_tag="JQH22_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFB2208000.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT         1511 a         1654 c         1551 g         1445 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca ccgcttcggc ctgtgcaccg gagactggcc
     2041 tgcaacaact ggttgccact cccgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 attttggcct cattccacag tgggtcaccc tggaaccccg tgtcttcggc tggatggacc
     2161 gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg aaacatgacg cccggcgagc
     2521 gcagtctcat tctgcgggcc ctgcaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     2641 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     2701 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     2761 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     2821 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     2881 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     2941 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     3001 gaggtatcac atcatgagaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
     3061 tcctgcatcc cgtctttttc cgttctgtac cggtaagtat cgctggcacg gcagtgctga
     3121 agcgtatact ggtcgtgaag tgcaggatat tcccggcgtt cttgccgtgt ttgcagaacg
     3181 ccgtaaggac agttttggcc cgtatgtccg gctgatgagt gtcaccctga actgatgttc
     3241 cgtcacccgg gaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccatt
     3301 ctcatcaatg catacatctt caccgaaacc ggtcagcgcg ccggctgacc tgtcatcgtt
     3361 aaaccattct taaccacttt tcagagagga ttttatcgtg tcagacaaac tctccgggat
     3421 aactcatccc gatgacaatc acgaccgccc ctggtggggg cttccctgca cagtgaggcc
     3481 ctgttttggc gctcgtctgg tgcaagaggg taaccgcctg tattaccttg ccgaccgcgc
     3541 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
     3601 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gatatcagca
     3661 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg
     3721 ttacgtttat ctggctgttt atccgacacc agcacccgca accacctcat aaccccgaag
     3781 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
     3841 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
     3901 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgtc gatgaacgtg
     3961 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcagttaat tttctcgtgg
     4021 aaaaatacgc gctggtgcgt accgaccagc cgggattcag cacctgtccc cgctctcagt
     4081 taataaacag tattgatatc ctccgggctc gccgggcgac cggcctgatg acccgcgaca
     4141 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgaaact
     4201 ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaacatag ggcgcattgt
     4261 cgttgacgtc gatggtattg agctcgctga actgattaac atggtctgcg ataacggcta
     4321 ctcccttcgt gttgttgatg aatctgacca gatctcagca gaatgcacgc caccatttgc
     4381 tacccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca acgcctggtt
     4441 gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc attttacagg
     4501 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     4561 ggggctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt atggcgtcag
     4621 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     4681 accaggaaca acatgaaatc attaaccacg gaaacagcgc tggatattct gattgcgtgg
     4741 ctgcaggaca atatcgactg cggatcggga attatctttg acaacgatga ggataaaacg
     4801 gattcagcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg taccctgcgt
     4861 catctgcagc ttctgcacca gaaccggtga agctcactca ccagacttca ttcctctgac
     4921 gccagcctgc acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     4981 gatatcacaa tctcccgtcc ggaagtggtc aacgggcata cggacgttat ctgctcaacc
     5041 agcattcgcc acattctggc tgtacgaaag agtacactgt tgcaaatcga cacacttatc
     5101 cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg gaggtaaaac tgccccggac
     5161 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc cgaaacagca
     5221 atgaaagcca ttacccggaa tcttgatcgc gaaatctggc gtgacctgat gcaacgttcc
     5281 gggatgctgt cattaatgga tacgcaggcc cgtgatacat ggtaccggtc actggagtac
     5341 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     5401 aataaggatg aggtgtttga gcgaggggtg atcaacgtct tcagagggct gaactggaat
     5461 tacaaaacca attgcccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     5521 tgggaccggt ggggataatg atatggtcac ccccacctta acagcagtac gctgttaagg
     5581 taaatccctt taacggagag accatcatgc agaacatcac acttattggt atcgatctcg
     5641 gaaagcactc cttccacatt cattgtcaag acaagtctgg taaggctctt ctgcgtaaaa
     5701 aatttacccg tactaaactg atgcagtttc tggcgacatg tccgccctca gttgttgtaa
     5761 tggaagcctg tgcgggtgca cactttatgg ctcgctgtat ctctgatttt gggcatgaaa
     5821 ctaagctgat ttctccacag ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg
     5881 ttgatgcaga agccatatgc gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
     5941 gaactgagac ccagcaggcc atgagggcgt tacaccgggt cagagaatcg ctcatcagag
     6001 ataaggtgaa aacaaccaac cagatccacg gtttcttgct ggaatttgga atcagcctgc
     6061 cgataggtga tgctgtaata aaacggttgt ctctcgtgtt ggctgaacac gaaatccccg
     6121 aatatctgag ccgccttttg gtgaggttgc atacccacta c
//
LOCUS       DADLDT020000181         6146 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000181 DADLDT020000000
VERSION     DADLDT020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.185"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..312
                     /locus_tag="JQH22_003849"
     CDS             <1..312
                     /locus_tag="JQH22_003849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208001.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="JQH22_003850"
     CDS             complement(388..852)
                     /locus_tag="JQH22_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFB2208002.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="JQH22_003851"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="JQH22_003851"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFB2208003.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JQH22_003852"
     CDS             complement(1721..2410)
                     /locus_tag="JQH22_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFB2208004.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JQH22_003853"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JQH22_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFB2208005.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JQH22_003854"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JQH22_003854"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFB2208006.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JQH22_003855"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JQH22_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFB2208007.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1550 a         1674 c         1535 g         1387 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgac
//
LOCUS       DADLDT020000182         6141 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000182 DADLDT020000000
VERSION     DADLDT020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.186"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..157
                     /locus_tag="JQH22_003856"
     CDS             <1..157
                     /locus_tag="JQH22_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208008.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="JQH22_003857"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="JQH22_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HFB2208009.1"
                     /translation="MLKFILDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="JQH22_003858"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="JQH22_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFB2208010.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="JQH22_003859"
     CDS             873..1244
                     /locus_tag="JQH22_003859"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HFB2208011.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="JQH22_003860"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="JQH22_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
BASE COUNT         1384 a         1581 c         2004 g         1172 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgaga ataaacttca gcatcagaat gactcccttt cctgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcac tggcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc acggcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa aactggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa cgccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa c
//
LOCUS       DADLDT020000183         6068 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000183 DADLDT020000000
VERSION     DADLDT020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.187"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            392..1420
                     /gene="torT"
                     /locus_tag="JQH22_003861"
     CDS             392..1420
                     /gene="torT"
                     /locus_tag="JQH22_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFB2208012.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1393..2085)
                     /gene="torR"
                     /locus_tag="JQH22_003862"
     CDS             complement(1393..2085)
                     /gene="torR"
                     /locus_tag="JQH22_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFB2208013.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2172..3344
                     /gene="torC"
                     /locus_tag="JQH22_003863"
     CDS             2172..3344
                     /gene="torC"
                     /locus_tag="JQH22_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFB2208014.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3344..5884
                     /gene="torA"
                     /locus_tag="JQH22_003864"
     CDS             3344..5884
                     /gene="torA"
                     /locus_tag="JQH22_003864"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFB2208015.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5881..>6068
                     /gene="torD"
                     /locus_tag="JQH22_003865"
     CDS             5881..>6068
                     /gene="torD"
                     /locus_tag="JQH22_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFB2208016.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1473 a         1519 c         1681 g         1395 t
ORIGIN      
        1 gtcattaccg tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac
       61 cactgcatca tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc
      121 cagtgcgttg acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt
      181 tacaggtcac taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg
      241 tcgcgttttt cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta
      301 tttatgtgat tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct
      361 agcataaagc cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc
      421 ccttttcatg ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca
      481 tttcacggtg caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct
      541 ttatcccagc ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc
      601 tcgccgctac ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac
      661 ccagcaagca caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag
      721 tagcacgacg tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact
      781 ggtaaatgct cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat
      841 gggctatcaa cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct
      901 gctgatgccc ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc
      961 agccattgcc ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga
     1021 aatccagcgc aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc
     1081 cggaacggcc attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct
     1141 taccgtggtg tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt
     1201 gattatggct gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat
     1261 caggcaatta caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac
     1321 gccgaaaaat gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc
     1381 ggtctatttt tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac
     1441 cagtaaatcc gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag
     1501 gtcagggttt tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat
     1561 ttcgcccgga ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat
     1621 cggctcgcca tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg
     1681 atagcagtag tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag
     1741 atttttcacc cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc
     1801 gcccatttcc agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat
     1861 ccccaccgtt gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa
     1921 gttgatatcc agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc
     1981 accgctcgcc gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc
     2041 ctgggtaacc ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc
     2101 attctgttca tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta
     2161 caggggttat tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg
     2221 tactggcgct ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg
     2281 ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg
     2341 tgtatgaaga atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat
     2401 gccatgactg tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga
     2461 gcaacgacat ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag
     2521 ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa
     2581 cctgtcgctc ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg
     2641 cacgtcagat gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta
     2701 ttgcccacca gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg
     2761 ccagtgctaa tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg
     2821 cgaaaggcga taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta
     2881 aacgtgacgg cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc
     2941 agcgtgtact cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg
     3001 tgcagcagca ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga
     3061 gcaagttgca ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga
     3121 tctgggccta tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg
     3181 aaatcgccca ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta
     3241 ccagtctcga taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg
     3301 acaccgcagg taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct
     3361 ctttcaggca tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat
     3421 gctggggccg tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc
     3481 tgtcatctcg aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt
     3541 gaaggatggt cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat
     3601 gatcgccgga ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg
     3661 cgtggactgg ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt
     3721 tgtgcgcgtg agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca
     3781 gaaaactcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
     3841 ccataacgct tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac
     3901 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat
     3961 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
     4021 gctgtggggc tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
     4081 tgtttatgaa tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat
     4141 cagcatcgat ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca
     4201 cattgcggtt aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta
     4261 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt
     4321 cctgccgtat ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
     4381 actgaccggc attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag
     4441 aacgcaaatt attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg
     4501 gatgattgtg gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg
     4561 ctggcactac aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc
     4621 cggctctacg tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac
     4681 tattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa
     4741 cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt
     4801 ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat
     4861 cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac
     4921 cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat
     4981 cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg
     5041 cgagctgtgc cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg
     5101 ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct
     5161 gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat
     5221 gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag
     5281 tggcctgatt gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg
     5341 gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta
     5401 tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc
     5461 ggaaacgctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca
     5521 ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg
     5581 tcaggtgttg gcaggagcag tagtttctga ccgctatgca cccggcgtgg cgcgaattca
     5641 cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg
     5701 taaccccaac gtgctgacca tcgacatcgg gacttcgcag ctggcgcagg cggccagtgc
     5761 gcacactacg ctggtggaaa ttgagaagta caatggaaca gtggagcagg tgacggcgtt
     5821 taacggcccc gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc
     5881 atgaccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc
     5941 tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg
     6001 ttttcgttgc tgaaaagcga accgccgctc actgcggcgg tgaatgatct ggaaaacagt
     6061 attgccac
//
LOCUS       DADLDT020000184         6052 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000184 DADLDT020000000
VERSION     DADLDT020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.188"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            430..1338
                     /locus_tag="JQH22_003866"
     CDS             430..1338
                     /locus_tag="JQH22_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFB2208017.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQH22_003867"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQH22_003867"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFB2208018.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="JQH22_003868"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="JQH22_003868"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFB2208019.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQH22_003869"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQH22_003869"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFB2208020.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="JQH22_003870"
     CDS             complement(5673..>6052)
                     /locus_tag="JQH22_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208021.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1575 c         1561 g         1425 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggaa atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DADLDT020000185         6028 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000185 DADLDT020000000
VERSION     DADLDT020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.189"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="JQH22_003871"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="JQH22_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFB2208022.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="JQH22_003872"
     CDS             1207..1869
                     /locus_tag="JQH22_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFB2208023.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="JQH22_003873"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="JQH22_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFB2208024.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="JQH22_003874"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="JQH22_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="JQH22_003875"
     CDS             2795..3100
                     /locus_tag="JQH22_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFB2208025.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="JQH22_003876"
     CDS             3103..3507
                     /locus_tag="JQH22_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFB2208026.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="JQH22_003877"
     CDS             3497..3796
                     /locus_tag="JQH22_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFB2208027.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="JQH22_003878"
     CDS             3982..4278
                     /locus_tag="JQH22_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFB2208028.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="JQH22_003879"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="JQH22_003879"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFB2208029.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="JQH22_003880"
     CDS             5627..5995
                     /locus_tag="JQH22_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFB2208030.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DADLDT020000186         5983 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000186 DADLDT020000000
VERSION     DADLDT020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.190"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..393)
                     /locus_tag="JQH22_003881"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="JQH22_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JQH22_003882"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JQH22_003882"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFB2208031.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGIIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="JQH22_003883"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="JQH22_003883"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFB2208032.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="JQH22_003884"
     CDS             5797..>5983
                     /locus_tag="JQH22_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208033.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1839 a         1354 c         1019 g         1771 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattatac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DADLDT020000187         5948 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000187 DADLDT020000000
VERSION     DADLDT020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.191"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(559..864)
                     /locus_tag="JQH22_003885"
     CDS             complement(559..864)
                     /locus_tag="JQH22_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208034.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(918..1169)
                     /locus_tag="JQH22_003886"
     CDS             complement(918..1169)
                     /locus_tag="JQH22_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFB2208035.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1248..2519)
                     /locus_tag="JQH22_003887"
     CDS             complement(1248..2519)
                     /locus_tag="JQH22_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFB2208036.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="JQH22_003888"
     CDS             complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="JQH22_003888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFB2208037.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3045..4265
                     /locus_tag="JQH22_003889"
                     /pseudo
     CDS             3045..4265
                     /locus_tag="JQH22_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4720..5685
                     /locus_tag="JQH22_003890"
     CDS             4720..5685
                     /locus_tag="JQH22_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HFB2208038.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1420 a         1576 c         1623 g         1329 t
ORIGIN      
        1 ctgggaccac ggtgccacct gtatcgccgg tcaggttatc tgcctgggac cacggtccca
       61 cctgcatcgt cggcacatta ttagttaggg acaacggttc aatcaatgcc tgtcacgaac
      121 aggtattggc ttgtcgccag caacccgttg agcggagtaa tcacgcagca ggacggctgt
      181 tacaggcgat ggtgaaagct ctgttgggat ctgaacgaag catgttgagt gaaatttgca
      241 aaatttcgca gagtatgcgg cttcatcttt aaatttccgc cagcattttt aaagccagct
      301 accgacgctg cgcgttggcc ggcccttcgt ccggcgggta accagtaccg gccgatctta
      361 cgaccctgga tgaacatcat cccgtcagca gcgtctccac tgtgatgtcg tttttcacac
      421 tgtccgatca caaaatagaa cgggcaggga caacatatgg taggaaaagc agctgccaga
      481 gacacaatat attgattacc cacagtaacg ggggataacc atgtgggtaa catattgaaa
      541 gtgcaacacg ttatctgatt aattgacttt gtaaacgccg ctgaaccatg aaaaaaaacc
      601 gggggaatca tcacagcgca taaacatacc gttttgtttt tgggtgacgg caaggaggcc
      661 ccttttatca ccacatacct tgtcaaaggt ttccatttct atccttatca ggctcttgtc
      721 gttattttca ggagagatcc ggagatgttt tgtcagccag acaggatagg tatcagcgtt
      781 acgccagtac tgcaattcaa tcactgaagc cattccccag acagcgaagt ataagaaaac
      841 cacacctgcg attttgctac ccatattgtt ccttaacatt ggcttaatac attccttgct
      901 cctcgcaccg gagcacatca cagatactga ttgagatcca cttcacgcag cggctggttt
      961 tgacgttctg ttatcgtttg caccaggtct ttttcgtcca gctcatcgag catttgttca
     1021 tacaaagccg cgggcacaca ataaaaaaca gggcggttac ggtcaattat ctcgaccggg
     1081 tagccgccgc gggcactggc ggtggccacc ggatcgtttt taagttcgct gacgctggca
     1141 gtcacatcac acagaatgat atttgtcatg gttcccccct gagacaagtc acctgtatga
     1201 tcggtaaagt aagctctggc ggcaagtccc ttctgtcatt tcaacggtca gaaacgggca
     1261 accggcagct ccttccagcg tgtggtatat gccggtgaca gcatctcccg tttcatctgc
     1321 cagtccggcg caataccccg tccggcaaac cagactttcc cgagtccgga atgattaatg
     1381 ccgtcgagta ccttcatcag cgcatcgctg tgcggccgtg gctgcacgtc atcgaacaga
     1441 ttcagctgcg cgacgccgtt cggactgaaa tcattgagca ttatccccgc ttttgcgtag
     1501 cggtggccat cgagccatat cctgtccaga gacctgaccg ctgccgcgat aatgtcccgg
     1561 gtgtcacggg taggggtgtt cagtttttcc gtggccacgt taccgtaata cggctcatta
     1621 accgcaaaag gggatgtctt gatgaaggtg ctgatatgcc ggcagtactg tcgctcaccg
     1681 cgcaactttt ccgccgcgcg ctcggcatac tggcagacgg cctggcgcat ttctccgtag
     1741 gtggtgatcc gctgcccgaa gctgcgactg cagacaatct gctgtttcgg cggcggcgct
     1801 tcctccagcg aaatgcaact ctcgccgttc agctcacgta ccgtgcgctc cagcactacg
     1861 ttgaagtttt tccggataaa tgtagggttc gtcagcgaca gctgcagggc ggtggtgatc
     1921 cccataacgt gcagcttttt cgctatccgg ttccccacgc cccagatttc ttccaccggc
     1981 tgcaggctga gtaattttgc cgttctgcgt ggattttcgg gcgacagcgc cagcacccca
     2041 ctgaattgtg gccattcctt tgacgcccac tgcgccgatt tggccagtgt ttttgtggcg
     2101 ccgaagccga caccgatcgt cagtccggtt ccactgagaa catgcccacg cagctgcctg
     2161 ccaaaatcct ccagatccat acagtggcta attccccggg catccaggaa acattcgtca
     2221 attgagtatt gttcgacgcg gggcgacagc tcttccagca gggcgaccac acggttactc
     2281 aggctcgcgt agagctcgta gttgctggaa aaaacgtaca gtttttcagg aaactgcgcc
     2341 tctttgagct ggaaccaggg ggttcccatc tttataccca gccgtttggc ttcagcactc
     2401 cgggcaataa cgcagccatc gttgttactg agaacgacga caggtttgcc gcgcaaatcc
     2461 ggcctgaaaa ccttctcgca actggcgtag aaggagttga cgtctgccag ggcgaacatc
     2521 agtctgccct ccgggtttta tgaatgaagg ccgtcaccac gccgaaaatt tgcagagttt
     2581 ccggatccgg gtacagggaa gggaagtccg ggttcatcgc ctgcagggct ggccgcggcg
     2641 tcagaagcag gcgttttacc gtaaattcac cctcaatttc cgcgataaca atatccccgt
     2701 gccgcggctg ttctgcttta tccacgacca tcagatcgcc ggaatagagg ccaatatcag
     2761 tcattgagtt gccaatcgct ctgacaaaat aggtggagtg gcgccggtga atgcagtact
     2821 cattcagatc cagttcagac tcagtatagt ttgctgccgg tgacggaaaa cccgcagggc
     2881 atcgctctgt gaacaaagga atttttacta tgctggcttc tgatactgca ggaatgaact
     2941 tcatcgtttt ttccctcgct taaaactgta tatgcatggg tcttcccttg ttgtggtggc
     3001 tgaaggcatg ataatggtgt atttaatcgc cagaggtcac cgccatggac gaaaagtccc
     3061 tctacgctca tattctcaac ctgtccgatc cgtggcaggt aaagtccctt tctctcgatg
     3121 aaaatgccgg ttctgttact gtcactattg agatcgctga aaacacccgg ctagcctgtc
     3181 cgacctgcgg taaatcctgt tctgttcacg atcaccgtca tcgtaaatgg cgccatcttg
     3241 atacctgcca gttcaccact attgttgaag ccgatgttcc acgaattatg tgtccggagc
     3301 atggctgcct gacgttgcct gttccgtggg ctggccccgg aagccggtat acgttgctat
     3361 tcgaatcgtt cgttctctca tggctgaaaa tcagcaccgt tgatgctgtc aggaagcaac
     3421 ttaagctcag ttggaatgcg gttgacggca ttatgacccg ggcagttaag cgaggtcttg
     3481 cccggataaa aaagccatta tccgcccgtc atatgaatgt ggatgaggtc gcctttaaaa
     3541 aaggacatcg ttacataacg gtgatctccg atcgcgatgg tcgggcgctg gccttaacgg
     3601 atgatcgcgg cacagagagt cttgccggct atcttcgcac gctcactgat gggcagttgc
     3661 tggctatcaa aacgctctca atggacatga acgcgggcta tataagagca gcgcgtatcc
     3721 acttacccag tgcggttgag aaaatcgcct ttgaccgctt ccatgtggcg aagcaactgg
     3781 gcgaggtagt tgataaaacc cgtcagaatg aacatccgca cctccctgtt gaaagccgac
     3841 accaggcaaa aggaacccgc ttcctgtggc agtacagcga taagtggatg accgaatccc
     3901 ggcaggaaaa gctgatgtgg ctgcgtgcac agatgaagct gacgagccag tgctgggcgc
     3961 tgaaagagct ggcaaaggat atctggaaca ggccatggag cgaggaaaga cggagtgact
     4021 ggcagagatg gttggcgctg gcggctaaca gtgacgttcc catgatgaaa aatgccgcga
     4081 aaacgatagg aaaaaggctg taggggatcc tgaatgcgat gcgacacagt gtctcaaacg
     4141 gaaatgcgga ggcacttaac agcaagatca ggctgctgag gataaaagcc aggggatacc
     4201 gaaaccggga gcgctttaaa ctgggggtga tgttccacta cgaaaagctg aatatggcgt
     4261 tctgagcctt cccaccatga tcggggaaga cccatatgca tacagtataa ggggcggtga
     4321 aatttctgca agtgatgggg ggatagccgg tcagaacgtc cccgaaaaca aaccgccccc
     4381 ggtttcagga agggcgaact gaccggcaga ggcagtgtaa caccgcatgt cacaaaggca
     4441 gcactgacgt cgtcagtgct ttttttaccc gtcctcccgg acgctgaagg gcagtttcac
     4501 ccgtgcagct aaaggggcgg taagtccggg tgtgcacatc gtcattttct cctgtgatgc
     4561 ctttctcctg cgtatcagct tccggtaatc gtcccccgtc acaacctgga cagcaagggt
     4621 aaatggcacg ctgcgcgccc ttgctgacca gaacgactcc gggcaggccg aaccgtcagg
     4681 cgatacgaag acgaaattca cactgacgga gaaacagcca tgacgatgaa cctgcacact
     4741 cagacaaccg cccctaaagc ttcacaggcg accagcgtat ccccgctgga gcagtcaggc
     4801 tcctcaaaaa cgaaattttc caggggcaaa agcaaagccc gtgctggcgc agtcaaaaca
     4861 gacctttacc agacagtaac ggacagcatc attgcagccc tggaaagcgg cgttaagccg
     4921 tgggtgtgtc cgtgggttcg caacggcgca gcggtgggat taccggccaa cttcagcacc
     4981 ggcacggcat acagcggaat taatatcatg ctgctgtggt gtagtgcggc aaaacagggt
     5041 tttcaggacg agcgctggct gacctacaaa caggcgcagg agctgggcgg ccaggttcgt
     5101 aaaggtgaac acggcacgac ggccatcttt tacaagacgc tggagaaaga ggacgaggac
     5161 ggggaaatcg aaaaaatccc gatgctgaaa gcctttaccg tgtttaacgt ggagcaaatc
     5221 gacggcctga gcatcgagag cgtgccgcag ccggttgccg gtttcgatcc gctgccgcag
     5281 gcggaagcac tcatgatccg gagcggggca aaaatcaccg agcagggcgt aaaggcgttc
     5341 tatcgtccgg ccaccgatga aatcgtcctg ccggagcggt tccgcttcgc ggacgcggcg
     5401 aacttctacg ccaccggcct gcatgagctg gttcactgga cgggtgcagc gtcccgcctc
     5461 aatcgtgaaa aaggcaataa gtttggctct gaggcgtatg cgtttgagga actgatcgcc
     5521 gagctgggca gcgcgtttct gttggcggat ttggggatta cgggagaggt gcagcatgaa
     5581 agctatatcg cgtcctggct gaaagcgctg aagggagaca agcgctacat ctttaaagcg
     5641 gcggcagcgg cctcaaaagc gcaccgctgg ctgatggact gctgacagct ggagccctgc
     5701 ggtgcagggc ttcagaattt ttgaaagaga gagaatcagg gagggaatta tataagtcac
     5761 tgttttttag aattctgtaa cgcggccggc ctcagctgca ggacgtcctg cagctgaggc
     5821 cggcacggaa aaataaaccg gacagattca aaaaaaatga accctttttc aggcggtagc
     5881 cgttacgtat ccgggatacg taacggcgcg atgtcttacg taaaaaatga cgtaacggct
     5941 ttcgccgc
//
LOCUS       DADLDT020000188         5851 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000188 DADLDT020000000
VERSION     DADLDT020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.192"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..355
                     /locus_tag="JQH22_003891"
     CDS             <1..355
                     /locus_tag="JQH22_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFB2208039.1"
                     /translation="LAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGD
                     VLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHREWHASA
                     FTGICNDLAAASTFV"
     gene            292..894
                     /locus_tag="JQH22_003892"
     CDS             292..894
                     /locus_tag="JQH22_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFB2208040.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            955..4435
                     /locus_tag="JQH22_003893"
                     /pseudo
     CDS             955..4435
                     /locus_tag="JQH22_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            4503..5102
                     /locus_tag="JQH22_003894"
     CDS             4503..5102
                     /locus_tag="JQH22_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFB2208041.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5166..>5851
                     /locus_tag="JQH22_003895"
     CDS             5166..>5851
                     /locus_tag="JQH22_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFB2208042.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNL"
BASE COUNT         1361 a         1426 c         1839 g         1225 t
ORIGIN      
        1 tctggcgggg attgagatgc cggattttca tcgcggggat gactggtggc gtcacggtca
       61 gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt tgtcatcagc
      121 acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc atgccgccat
      181 ttactgcggc gatggtgagc tgctgcacca tattcctgaa caactgagta aacgagagag
      241 gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg aatggcacgc
      301 atctgccttt acggggattt gcaacgattt ggccgccgca tcgaccttcg tgtgaaaacg
      361 ggggctgaag ccatccgcgc actggccaca cagctcccgg cgtttcgtca gaaactgagt
      421 gacggctggt atcaggtgcg cattgccggg cgtgatgcag gtgaaaccga attgtctgcc
      481 cgtcttaatg agccgctggc aaatggtgca gtgatccaca tagtaccgcg tctggtggga
      541 gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag ctgttatggc ggttgctata
      601 tggatgccgg gggtaggaat tatggcgagt aatctgctgt tttctctcgg tgccagtatg
      661 acgcttggcg gtgttgcaca gatgctggca ccgaaagcca gaactccccg tacacagaca
      721 acggataacg gcaaacagaa cacctgtttc tcctcactgg ataacatggt tgcccagggc
      781 aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt cgcgggtggt atctcaggag
      841 atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg ctgatgcaaa
      901 atgtttcatg tgaaaccgcc tgcgggcggt tttgtcgttt atggagcgtg aggaatgggt
      961 aaaggcagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa atccacgcag
     1021 ttgctgagtg tgattgatgc catcagcgaa gggccggttg acggtccggt ggatggatta
     1081 aaaagcgtgc tgctgaacgg tacgccggtc ctggatagcg aggggaagac aaacttttcc
     1141 ggtgttacgg tggtgttccg cgccggcgag caggagcaga caccgccgga ggggtttgaa
     1201 tcttccggct cagagactgt gctgggtacg gaagtgaaat acgacacgcc gatcacccgg
     1261 accatcacgt cggcaaacat tgatcgtctg cgcctgacct tcggtgtgca ggcactggtg
     1321 gaaaccacct caaaggggga ccggaatccg tcggaagtcc gcctgctggt tcagatacag
     1381 cgtaacggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacttcgcag
     1441 tatctggcct cggtggtggt gggtaacctg ccgccgcgcc cgtttaatat ccggatgcgc
     1501 aggatgacac cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcgtac
     1561 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgctggtcgg cgtgcaggtg
     1621 gattcagagc agttcggtaa ccagcaggtg agtcgcaatt atcatcttcg cgggcgcatt
     1681 ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat ctgggacgga
     1741 acgcttaagc cagcatacag caacaacatg gcctggtgtc tgtgggacat gctcactcat
     1801 ccgcgctacg gcatggggaa acgtcttggt gcggcagatg tggacaaatg ggcgctgtat
     1861 gtcatcggcc agtactgcga ccagtcagtg ccggacggat ttggcggcac ggagccgcgc
     1921 atcacctgta acgcttacct gaccacacag cgtaaggcgt gggatgttct cagtgatttc
     1981 tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac
     2041 cgaccgtcgg ataaagtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggc
     2101 gcgccgttcc gctacagctt cagcgccctg aaggaccgcc ataatgtcgt tgaggtgaac
     2161 tggattgatc cggacaacgg tcatgagacg gcgacagagc ttgtggagga cacgcaggcc
     2221 attgtccgtt acggtcgtaa cgtcacgaag atggatgcct ttggctgtac cagccgggga
     2281 caggcgcacc gcgccgggct gtggctgatt aaaacggaac tgctggaaac gcagaccgtg
     2341 gacttcagcg tcggcgcaga agggcttcgc catgtaccgg gtgatgtcat tgaaatctgc
     2401 gatgatgatt atgccggtat cagcaccggt ggtcgcgtac tggcggtgaa cagccagacc
     2461 cggacgctga cgctcgaccg tgaaatcacg ctgccgtcct ccggtaccac gctgataagc
     2521 ctggttgacg gaaatggcaa tccggtcagc gtggaggttc agtccgtcac cgacggcgtg
     2581 aaggtgaaag tgagccgggt tcctgacggc gttgcagaat acagcgtgtg ggggctgaag
     2641 ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta tccgtgagaa cgacgacggc
     2701 acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag aggccatcgt ggataacggg
     2761 gcacactttg acggtgaccg gcgcggcacg gtgaatggtg tcacgccgcc agcggtgcag
     2821 catctgaccg ccgaagtcac cgcagacagc ggggaatatc aggtgctggc gcgatgggac
     2881 acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc tgaccgtggc agcggacgac
     2941 ggcagtgagc ggctggtcag cacagccagg acgacggaaa ccacataccg cttcaggcag
     3001 ctggcgctgg ggaattacag tctgacagtc cgggcggtaa atgcccgggg gcagcagggc
     3061 gatccggcgt cggtatcgtt ccggattgcg gcaccggcag cgcctgtcac tattgaactg
     3121 ataccggggt attttcagat aacggtggtc ccgaaactgg ctgtatatga cccgacggtg
     3181 cagtttgagt tctggttttc ggaaaagcgg attgcggata tcaggcaggt tgaaaccagc
     3241 gcccgctatc ttggcacggc gctgtactgg atagccgcca gtatcaatat caggccgggc
     3301 catgattatt atttttacgt tcgcagtgtg aacaccgttg gcaaatcggc atttgtggag
     3361 gccgtcggtc gggcgagcga tgatgcggaa ggttatctga gtttttataa agggttgatc
     3421 aataaaacgc atctcggcaa ggagttgtgg acgcagattg ataacggtca gcttgcgccg
     3481 gacctgactg aaatcaggac gtccattacg aatgtcagca atgaaatcac gcaaaccgtc
     3541 aataaaaaac tggaaaatca gagtgctgca atccagcaga tacagaaagt tcaggttgat
     3601 acaaataata acctgaacag catgtgggct gtgaagctgc aacagatgaa ggacggacgc
     3661 ctttatattg cgtggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca
     3721 ggtgctgctg gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc
     3781 gatgtttgtt ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac
     3841 ggctccgacc attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg
     3901 gctgacggcg aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa
     3961 caacgtcacg attaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga
     4021 aggcgatctc gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag
     4081 gtggccatca gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat
     4141 tgttattccc gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta
     4201 ttcgtcatgc cgcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct
     4261 ggataatacg ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac
     4321 gctggagttt tctgtatcgg catggctggt aaatggctgg tatcccacag caagtatcag
     4381 cgatttgctg gttgttgtga tgaagaaatc cacagcaggt atcagtatca gctgaatttt
     4441 ataacccaga tacgggcacc agaaatggtg ccttttttat tgcagaaaag cgagaggtaa
     4501 ttatgcgtaa actttatgct gccattttgt ccgcagccat ctgtctggct gtatccggtg
     4561 cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc ggggtatctt catgcctcga
     4621 cgaacgctcc cggcagtgat gatctgaacg ggattaacgt gaaataccgt tatgagttta
     4681 cggacacgct ggggctggtg acgtcattca gctatgccaa cgctgaagat gagcaaaaaa
     4741 cgcattacag tgatacccgc tggcatgagg attccgtgcg taaccgctgg ttcagcgtga
     4801 tggcggggct gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggcgtgg
     4861 cttacagccg tgtgtcgacc ttctccgggg attatctccg cgtaactgac aacaagggga
     4921 aaacgcacga tgtgctgacc gggagtgatg acaaccgcca cagcaacacg tctctggcgt
     4981 ggggggctgg cgtgcagttt aacccgaccg aatccgtggc cattgacctt gcttatgaag
     5041 gttccggcag tggcgactgg cgcactgacg gtttcatcgt gggtgtcggt tataagttct
     5101 gattagccag gtaacacagt gttatgacag cccgctggtt caggcgggct ttttgtgggg
     5161 tgaatatggc agtaaagatt tcaggtgtac tgaaagatgg ggcgggtaaa cctgtcgtaa
     5221 attgtgcgat tgaactgcgg gccagaagaa ccagtccgac cgttgtggca cacgttgttg
     5281 ccacttgcgt gacggacaat aacggtgctt atgtgattga ggctgagccg gggtattacg
     5341 aggttgcgct tcactgtaac ggctggcagc caacccgtgt cggggatatt gatgtggcac
     5401 cgactgatgc accggggaca ctgaacgcgt ttctgaatgc accaaaggat ggtgatttac
     5461 gtccggaggt gatgaagcgc tttgaggaaa tggtggcgca ggcgcagcag agtgccgggg
     5521 ctgcagccgg aaacgcacag cagacggcgc aggatgtggc ggcagccgca acggcccgtg
     5581 atgatgcaca acgttttgcg gagaaagccc gacaggatgc aaccgtcaca gctgaggaca
     5641 gaaaggccac tgcggaagat gtgacaagca caggagcaaa tgcagccgca gccggacaga
     5701 gcgcacagga tgccgcaggt tatgcccgcg cagcagaaca ggccaaaaat gacattgatg
     5761 ctgcgctgac cggcactctg aaaatggcta accatctgtc agaaatcgca gcagcaggcg
     5821 aaaaggcaca acagaagtcc cgggataatc t
//
LOCUS       DADLDT020000189         5841 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000189 DADLDT020000000
VERSION     DADLDT020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.193"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..541)
                     /locus_tag="JQH22_003896"
     CDS             complement(<1..541)
                     /locus_tag="JQH22_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFB2208043.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLE"
     gene            659..988
                     /locus_tag="JQH22_003897"
                     /pseudo
     CDS             659..988
                     /locus_tag="JQH22_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            complement(1794..2804)
                     /locus_tag="JQH22_003898"
     CDS             complement(1794..2804)
                     /locus_tag="JQH22_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFB2208044.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            3545..4711
                     /gene="sopA"
                     /locus_tag="JQH22_003899"
     CDS             3545..4711
                     /gene="sopA"
                     /locus_tag="JQH22_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFB2208045.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            4711..5682
                     /locus_tag="JQH22_003900"
     CDS             4711..5682
                     /locus_tag="JQH22_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFB2208046.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
BASE COUNT         1404 a         1455 c         1461 g         1521 t
ORIGIN      
        1 cttcgagcca gacttcgcac gggtcattca ggttccagcc gctgagcagg atgttgtcgc
       61 ccggcgcttt tttatcgcct tccaagcaaa acgaggtgta ggcgatgatg atgccgtcga
      121 ggatctcccg cagggtcatg gcggcggcga cgtttgccga cacctgactg cgcagcggac
      181 gcagcacgtt cgcgaccagt tctggtcttg ggtactcgcg tcctgcgtcg taaatcatct
      241 gacgcaggga ctcgattttg ccgtcctgca ggcgctgtaa catgctctgc tgcagcgtga
      301 gccagttgtt ggtcaggcgc gacgtagggc gctccagcag cggtttgacc tggccaatcg
      361 ggacgccttt ttttacccag tcgaggattt ttagcgcctg ctggacgtcc tcatcgctgt
      421 actggcggtg gccgccctcc gtgcgctgag gcttgagtaa gccgtagcgc cgctgccagg
      481 cccgcagcgt ggtggcggtg atcccgctga gtctggcgaa ttcgccgatg gagtaagcca
      541 tgtccgtgtc cagagttgag gaatactctc aatatactac gaattttcgc ggatgggatg
      601 aagcgccgcc gcggtgcact acacttaatt tccaaccgtg caaatgaaag gaattctcat
      661 gaagctatgg cctgttgtga ccggtgttgc cattgccctg acgctggtag cctgtaaatc
      721 ccctacccca ccgaagggcg tgcagcccat ttccggtttt gacgccagcc gctatcttgg
      781 caaatggtac gaagtcgctc gcctggaaaa ccgctttgaa cgcggcctag agcaggttac
      841 cgccacctac ggcaagcgca gcgacggcgg catcagcgtg cttaaccgcg gctacgaccc
      901 tgtgaagaac aagtggaatg agagcgaggg taaagcctac tttaccggcg aaccgaccac
      961 ggccgcgctg aaggtgtcct tcttggggtc agtttggata tagagaatta ttgtacggta
     1021 agcctgtttt ttgaacaggc ttacctgtag caatagggtg tactcccgcg ctatacagcg
     1081 ttttcagggg attttgtctt aatcggataa cctaaatgcg atacagcaag agcgacaacc
     1141 taaaagcgat agccaatgac gataaatcaa tcacttacgt atccgaattg gttaaataat
     1201 gtgcttaacg tacaaaaatt tccgatctcc aaactgaccc ctaaataggc tgattcgatg
     1261 tgtttgcggg aaaaaaatcg gcccagatcc gcgaaatttt aatcagcgag tcagcttggg
     1321 aagaaataac ctgcttattc gcaccttccc tagccaacac ctttctgcca ttcggctccc
     1381 gtttcctgat cgcatttaac agtgtgaagc tggcgccggg ttatacagac agctgcagtc
     1441 tcatcctgcc gggctaaacc tgcagtacac aggggaacgg ataccgtcag gtaacatgac
     1501 cgtctcagca cccgattgag attttcgtaa gggatataga gcgttactga aaaattgact
     1561 gtggcatgtt atcccccatg gaatggtgaa cagaccaggg attaccccca ttaaacccgc
     1621 attccgtagt caatatctgc gtatcatgac ccgaaatcac atatgacagt caatattgtt
     1681 cgatccgggc agacatgata gagaataaat attgactgcg gtatgcgtga tgcatatcac
     1741 agtcaatgga atgtccttag cggcatacgg cagtcatgat atgcatatcg cagtcaattt
     1801 tctggcttca tcgctgagag ggcttcagcc agtttaagag gatcgatgcc agcagccttc
     1861 agcgccttaa tgacttcgtc ataattttgc tctggcgcct tctcttcttc ctctttgcgc
     1921 ggaaccttca caggactact gatgagtttg gggttgcgat aatgcacgct gaaataggtg
     1981 gctcggccac gtttaaactc ggtgtaatcc agataaccaa tatcacgcag ctgctccatc
     2041 gccttacgca ccgtgtggtt ctgtgtatag acatttgagg tgagattcag ccggtcacgc
     2101 atccgtttca tggaaatagg tgcaggattc tggggaagac tttcgatgta ggtataaagc
     2161 gcctgggctg attccttgcg ctgcagggcg tcaatggctt tcagccgcag cagtaccctt
     2221 ctgtccatgt ggtaaagctc aaacagctta ggttcagcct tgatctcaac gatatcctcg
     2281 ttgatgtcat aataggctga ctgcaccaga tgggtggtcc atcctttggt ctcgctctgg
     2341 aacttcagcg taaccgaggc gagtttaaac agggaattac tgatgcgatc gcgcattttt
     2401 ttgtttgaac gacgggagtc aaatccgcac atcttaacga attcgacgaa cgacagttcc
     2461 agggtgtcac tttttacccc atactccgac atggagcgaa ttatccccag ccagacttta
     2521 aaatccgtgt ccatatcaag acgcgaaccg gtgatcttaa cgtcagtgta tccttcgctt
     2581 ttggcaatgg ccagctgtac aagctcctca gtcgcgtctg taacgttttt ccggttcgcc
     2641 ttgctcttcc ctgttgattt cagagttggg acgaagagac cgagtctcat gagcgctact
     2701 ggctgcacgg tactggtgct gttgacatcc agcttaacaa cttcaccggt tgtcttatca
     2761 acttcctgaa ggttcagaag taagctatta ttttcgctcg tcatcttgat cctcaacggt
     2821 tatccacaaa aggatgtagt aatacgtatc gcagtcaacg gatatacata cctcagtaaa
     2881 taccgttgca tatcacagta ttagattatg catataacag tcaacatttt acattccgca
     2941 gtcgatccag acagacgtta aagccagtta cagcgcatgc tggagcgatc cggggatctc
     3001 ttttggatct cactatgatc tccttgggat caattattgg atcgagacca tgaataaccc
     3061 agatacaagg ccggaagcgc cacaccagat atccagaccc ctggcctgat tttcctgaat
     3121 acgctctgga agattgagat ctacaggtac aacaatgaaa cattgactgc ggtatgctga
     3181 gtcgtctttc ctacagatgc ggtaagcatc ataccatagt caatatatta cataccgcag
     3241 tcaatttcat tataactggc tgatcccgag tcatctttag caacccgacg tacgtgcaat
     3301 ctgtgagttc tgtgattttc cgcttcctcg ccgctttctc actgtgtgag atcgcttact
     3361 gcggcgagcg gcagtgatta atattgactg cggtatgtac tcttttgtaa tcccctgcgt
     3421 tctttcatta cccaatgtgg ttcactttgc actttttcgt gtgctttgcg attttattgt
     3481 tgctttgcaa taaattccaa gatttaataa aataatgcaa agtgatgatt aaaggatgtt
     3541 cagtatggga ctcatggata cactcaacca gtgcataagt gctggtcatg aaatgacgaa
     3601 agcaatcgcc attgcacagt ttaatgatga cagtccggaa gcccggaaaa taacccggcg
     3661 ctggaggatt ggtgaagcgg cagatttagt tggcgtgtca tctcaggcta tcagggatgc
     3721 cgagaaagct ggccggctgc cgcaccctga tatggaaacc cgtgggagag ttgagcaacg
     3781 cgttggttat accatcgaac aaatcaatca catgcgggat gtatttggta cacgactgcg
     3841 ccgtgctgaa gatgcgtttc cgccggtgat tggcgttgca gctcacaaag gtggtgttta
     3901 caaaacctca gtatccgttc acctggctca ggatcttgct ctgaaaggac tccgtgttct
     3961 gcttgttgaa ggcaatgacc cacagggaac tgcttctatg tatcacggat gggtgcccga
     4021 tcttcatatt catgctgagg acactctcct gcccttttat cttggggaaa aggatgacgc
     4081 cagctacgct ataaagccca cctgctggcc tggccttgat atcatcccgt cctgtctggc
     4141 actgcaccgc attgaaaccg agctgatggg gaaatttgac gaaggcaaat tacctgctga
     4201 cccgcacctg atgctccgtc tggccattga aactgtcgct catgattatg acgtgatagt
     4261 tatcgacagt gcacccaacc ttggtatcgg tacgataaat gttgtatgcg ctgctgatgt
     4321 tctgattgtc cctactccgg cagagctgtt cgactacacc tccgcactgc agtttttcga
     4381 tatgcttcgt gacctgctta agaacgtgga tcttaaaggc ttcgagccag atgtccgtat
     4441 tctgcttacc aaatacagta ataataatgg ctcacagtcc ccgtggatgg aagaacaaat
     4501 tcgggatgcc tggggaagca tggtccttaa aaatgttgtg cgcgaaacgg atgaagtcgg
     4561 taaaggtcag atccgtatga gaaccgtttt tgaacaggct attgatcaac gttcttcaac
     4621 cggtgcctgg cgtaatgccc tctctatctg ggagcctgtt tgcaatgaga tttttgatcg
     4681 tctgattaaa ccacgctggg agattagata atgaagcgtg ctcctgtcat tccaagacat
     4741 accacacata gtcaatcgac tgaagatact tcatcaccgg cgccggccgc gccgatggtg
     4801 gattcattaa ttgcgcgcgt gggtgctatg gcccgcggta atgcaatctc tcttccggta
     4861 tgtggacggg aagtgaaatt tacccttgag gtgctccggg gagactctgt tgagagcgct
     4921 tcacgcgtat ggtcaggcaa tgagcgcgat caggaactac ttaccgaaga tgctctggat
     4981 gatctcattc cttcattttt actgaccggt cagcagactc cagctttcgg ccgccgggtt
     5041 tctgatgtca tagaaattgc cgacggcagc cgtcgccgta aagccgcaat cctgacggaa
     5101 agtgattatc gcgttctggt tggtgaactg gacgatgagc agatggctgc attgtcccga
     5161 ctgggtaacg attatcggcc gacgagtgct tatgaacgtg gcctgcgtta tacaagccgg
     5221 ctgcagaatg agtttgcagg aaatatttct gcgcttgccg atgcggaaaa catctctcgt
     5281 aagatcatta cccgctgtat caatacggcc aaattgccta aatccgttgt tgccctgttt
     5341 gcacatcctg gagaactgtc tgcccggtca ggtgaagccc ttcagaaggc ttttgcagat
     5401 aaagaagaat tactgaagca gcaggctgag actctccacg atcagaagaa agcgggacta
     5461 atctttgaag ctgaagaggt cattagtctt ttaacatccg tactgaaaca atctcccgca
     5521 tcaagagtta atctgagctc acgtcatcag tttgctccag gggcaacagc attgtataag
     5581 ggcgataaaa tggtgcttaa cctggatagg tcccgcattc ctgcggagtg tatagagaaa
     5641 atagaagcca ttcttaagga gcttgagaaa ccaggagtct gatgcggaca cgctttagtc
     5701 tacggtgatc tccctttact tcatttcctt tctcacaggc cagaaaacat aactggcctg
     5761 aatattctct ctgggaccac ggtcccacct gtatcgccgc tcaggttatc tccctgggac
     5821 cacggtccca cctgcatcgc c
//
LOCUS       DADLDT020000190         5825 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000190 DADLDT020000000
VERSION     DADLDT020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.194"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..1458)
                     /gene="fadB"
                     /locus_tag="JQH22_003901"
     CDS             complement(<1..1458)
                     /gene="fadB"
                     /locus_tag="JQH22_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFB2208047.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGK"
     gene            1648..2979
                     /gene="pepQ"
                     /locus_tag="JQH22_003902"
     CDS             1648..2979
                     /gene="pepQ"
                     /locus_tag="JQH22_003902"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFB2208048.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            2979..3593
                     /locus_tag="JQH22_003903"
     CDS             2979..3593
                     /locus_tag="JQH22_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFB2208049.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            3632..5083
                     /gene="trkH"
                     /locus_tag="JQH22_003904"
     CDS             3632..5083
                     /gene="trkH"
                     /locus_tag="JQH22_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFB2208050.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            5095..5640
                     /gene="hemG"
                     /locus_tag="JQH22_003905"
     CDS             5095..5640
                     /gene="hemG"
                     /locus_tag="JQH22_003905"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFB2208051.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1312 a         1492 c         1584 g         1437 t
ORIGIN      
        1 cttgcccatc ttgctcgccc aggcgacaac tttcgcgatg gtttcgtcgg agcttttctc
       61 gccgcgaata atttctacca acggcattcg gtggaccggg ttaaagaagt gcatcccgca
      121 gaagttttcc gggcgttcca gcgcgttggc cagttcgctg ataggaatgg ttgaagtgtt
      181 agacgccagc acggtatcct ggcgtacttt ttgttcggtt tctgccagta cggctttttt
      241 cactttcggg ttttcaacaa ccgcttctac cacaatatcc acgcggtcaa atccggcgta
      301 gtcgagcgtt gggtggatcg tggagatcac gccagccagt ttcagaccat cgattttgcc
      361 gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc atgccgaggg ttaacgactt
      421 gtcgttgata tctttcatga caaccggcac gcctttccac gcggactggt aagcgatacc
      481 gccacccatg atgcctgcgc ccagcacagc agcctgtttc ggggtttcaa cgtctttggt
      541 gagtttcttc gctttgcctt ttacatattg atcgttaagg aaaatgccga ccagtgcgcg
      601 agcttcgttg gtatgcgcca gcgggacaaa acttttgttt tccagattta aggcttcttc
      661 acgaccaaaa cgggccgcag cttcaatggt ttttactgcg gtgatggggg ccggataatg
      721 tttccccgcc gtttgtgcga ccattccttt agcgatggtg aagctcatgg tggcttcaat
      781 cttgctcagt tttagcggtt cctgcttcgg ctgacgtttc gctttccagt cgaggtcgcc
      841 gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc agtttttctg ctttgactac
      901 gccatccacc agaccgattt tcagcgcctg atccgcgccg acatctttac cggcggcaat
      961 gatttccagt gcactgtcag cgcccagcat acgtggcata cgtacagaac cgccaaagcc
     1021 cggcatgatg cccagtttgg tttccggcag accgatgcgc agatccggcg tcgccagacg
     1081 ataatcggtc gccagcacgc attcgcagcc accacccagc gcatagccgt tgacggcagc
     1141 aatggtcggc accggcagat cttccaggcg attaaacacg ctattggcaa agtgcagcca
     1201 ctgacttaac tgttcttcag gaacgaggaa cagggacaaa aattcggtga tatcagcacc
     1261 gacgataaag gctgctttgt tcgaacgcag cagcagccct tttagatctg attgctgttc
     1321 cagcacgccg atggcctcgc cgaggctggc gacggtcgca gtgtcgagtt tattaactga
     1381 acctggggca tcaaatacca gttcggcaat gccatcttcc agccagtcaa ggtacagggt
     1441 gtcgcctttg taaagcatgt cagtctcctg aatccgcaag gtgatctggt cgtaccagat
     1501 gcatcggagt gtgtgttttg tgttaaaaat atgcaaataa aagattaaag aaatgccgat
     1561 ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat gtgctaagat gcggagactt
     1621 aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg cctcgctcta taaaaatcat
     1681 atagctacct tacaagaacg gactcgcgat gcgctggcgc gcttcaagct ggatgcatta
     1741 cttattcact ccggcgagct gttcaacgtt tttctcgacg atcatcccta tccgtttaaa
     1801 gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg tgccaaactg ctggttgctg
     1861 gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc cggttgatta ctggcacaac
     1921 gtcgaaccgc tgccgacctc cttctggact gaagatgtgg aagtgatcgc gctgccgaaa
     1981 gccgatggca ttggtagcct gctgcctgct gcgcgcggca atatcggtta tatcggtccg
     2041 gtgccggaac gtgcgctgca actgggtatt gaggccagca acatcaaccc gaaaggggtg
     2101 atcgactacc tgcattatta ccgctccttc aaaaccgagt acgaactggc ctgtatgcgt
     2161 gaagcgcaga aaatggcggt caatggtcat cgtgcggcag aagaagcgtt ccgttctggc
     2221 atgagcgagt tcgatatcaa tattgcctat ctgaccgcga ccggtcatcg tgataccgac
     2281 gtaccttaca gcaacattgt ggcgcttaac gaacacgctg cggtgttgca ttacaccaaa
     2341 ctggaccacc aggcaccgga agagatgcgc agcttcctgc tggatgccgg ggccgaatat
     2401 aacggctatg cggctgacct gacccgtacc tggtcggcaa aaagcgacaa cgactacgca
     2461 cagctggtga aagacgtaaa tgatgaacaa ctggcgctga tcgccaccat gaaagcaggc
     2521 gtcagctatg tggattacca catccagttc catcagcgca tcgccaaact gctgcgtaaa
     2581 catcaaatca tcaccgatat gagtgaagaa gcgatggtcg aaaacgatct caccggaccg
     2641 tttatgccgc acggtattgg ccatccgctg ggcctgcagg tgcatgacgt cgccggtttt
     2701 atgcaggatg atagcggtac acacctcgcg gcaccggcaa aatatccgta cctgcgctac
     2761 acccgtattc tccagccggg catggtgtta accatcgaac cgggtatcta cttcattgaa
     2821 tcgcttctgg caccgtggcg tgaagggcag ttcagcaagc acttcaactg gcagaaaatt
     2881 gaagcactga aaccgttcgg cggcattcgt atcgaagaca acgtggtgat ccacgaaaac
     2941 aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat ggaaagctgg ttaattcctg
     3001 cggcaccggt cacggtcgtt gaagagatca aaaagagccg cttcataacg atgttggcgc
     3061 ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc ggtgcgggca gaacaccccg
     3121 atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc ggatgattct caacagctgg
     3181 gtttctctga cgacggggag ccggcgggaa cggcaggtaa accgatgctc gcccagctaa
     3241 tgggcagcgg cgtcggggaa attaccgctg tggtagtgcg ctactacggc ggcatattgc
     3301 ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt gaatcaggcg ctgcgccagc
     3361 taacgaccca acgcaagacg ccattaaccg aatatacttt gcaatgtgaa tatagtcagt
     3421 taaccggcat tgaagcgttg ctggggcagt gtgacggcaa aattatcaac agtgattatc
     3481 aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa agtggctgaa ttttccgcaa
     3541 agctggcgga ttttagccgt ggttcattgc aattgttagc gattgaagaa taatccccac
     3601 ttcgttttgc agaactaagg aagcggcaga gatgcatttt cgcgccatta cccgaatcgt
     3661 tggactactg gtcatcttat tttcagggac catgattatc cctgggctgg tagcactcat
     3721 ctaccgggat ggagcgggcc gcgcttttac ccagaccttt tttgtcgccc tcgccattgg
     3781 ctctatgctg tggtggccga accgcaaaga gaagggcgaa ctgaaatccc gtgaggggtt
     3841 tctgatagtg gtgctgtttt ggaccgtgct gggtagtgtc ggtgcgctcc cttttatctt
     3901 ctcggaaagc ccgaacctca cgattaccga tgcgtttttt gaatctttct ctggcctgac
     3961 caccaccggg gccactacgc tggtggggct ggattcgctc cctcatgcca tcctctttta
     4021 tcgccagatg ctgcaatggt ttggcgggat ggggatcatc gtgttagcgg ttgcgatact
     4081 gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca gaaatgcccg gcccgctgaa
     4141 agataacaaa atgcgcccgc gaattgcgga aacggcgaaa actctgtggt tgatttatgt
     4201 cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc ggaatggatg cctttgatgc
     4261 catcggccat agctttgcga ctatcgctat tggcggcttc tcgacacatg atgccagtat
     4321 cggttatttc gacagtccga ctattaacac tatcattgct atcttcctgc tgatctccgg
     4381 ctgtaactac ggtctgcact tttcactgtt aagtgggcgt agtctgaagg tttattggcg
     4441 cgatccggaa tttcgcatgt ttatcggcgt acagtttacg ctggtggtta tttgtacact
     4501 cgtactgtgg tttcataatg tctacagttc ggcgctgatg acaattaacc aggcgttttt
     4561 ccaggtggta tcgatggcga caaccgccgg gtttacgact gacagcattg cccgctggcc
     4621 gctctttttg ccggtactgc ttttatgttc agcttttatc ggcggttgcg ccgggtcaac
     4681 gggcggtggc ctgaaagtga tccgcatcct gctgctgttt aagcagggga accgtgagct
     4741 gaaacgactg gtgcatccga acgccgtcta tagcattaag ctggggaatc gcgcactgcc
     4801 ggaacgtatc ctcgaagccg tttggggatt tttctccgcc tatgcattgg tgtttattgt
     4861 cagtatgctg gcgattatcg ccacgggcgt ggatgatttt tctgcctttg cctcagttgt
     4921 tgcgacattg aataacctgg ggccagggct tggcgtggtt gctgataact ttaccagtat
     4981 gaacccggtg gctaaatgga tcctgattgc caacatgctg tttggtcgtc tcgaggtctt
     5041 tacattactg gtgctcttta ccccgacttt ctggcgtgaa tgatggagta atacgtgaaa
     5101 acattaattc ttttctcaac aagggacgga caaacgcgcg agattgcctc ctacctggct
     5161 tcggaactga aagaactggg gatccagacg gatgtcgcca atgtgcaccg cattgaagaa
     5221 ccacaatggg aaaactatga ccgtgtggtc attggtgctt ctattcgcta tggtcactac
     5281 cactctgcgt tccaggaatt tgtcaaaaaa catgcgacgc gactgaattc gatgccgagc
     5341 gccttttatt ccgtaaacct ggtggcgcgc aaaccggaga agcgtactcc acagaccaac
     5401 agctacgcgc ggaagtttct gatgaactcg caatggcgtc ccgatcgctg cgcggtcatt
     5461 gccggggcgc tgcgttaccc acgttatcgc tggtacgacc gttttatgct caagctgatt
     5521 atgaagatgt caggcggtga aacggatacg cgcaaagaag ttgtctatac cgattgggag
     5581 caggtggcga atttcgcccg agaaatcgcc catttaaccg acaaaccgac gctgaaataa
     5641 gcataaagaa taaaaaatgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
     5701 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
     5761 cagcggggtt ctcctgagaa ttccgacaga gaaagcaaaa ataaatgctt gactctgtag
     5821 cggga
//
LOCUS       DADLDT020000191         5822 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000191 DADLDT020000000
VERSION     DADLDT020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.195"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            105..695
                     /locus_tag="JQH22_003906"
     CDS             105..695
                     /locus_tag="JQH22_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFB2208052.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            832..1404
                     /locus_tag="JQH22_003907"
     CDS             832..1404
                     /locus_tag="JQH22_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208053.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            1441..2832
                     /locus_tag="JQH22_003908"
     CDS             1441..2832
                     /locus_tag="JQH22_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFB2208054.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            2877..3293
                     /locus_tag="JQH22_003909"
     CDS             2877..3293
                     /locus_tag="JQH22_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208055.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(3612..4268)
                     /locus_tag="JQH22_003910"
     CDS             complement(3612..4268)
                     /locus_tag="JQH22_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFB2208056.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            4663..5678
                     /locus_tag="JQH22_003911"
                     /pseudo
     CDS             4663..5678
                     /locus_tag="JQH22_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1509 a         1389 c         1534 g         1390 t
ORIGIN      
        1 tgttacaaga tggagagcat gaaaaacctc ggggcagcca tcgaatgatg ccatctacag
       61 gggttaaaat aaccaccatc atttcataac aatacctgct gactatgaag atctacgtca
      121 tcaaaatagc agttcatgga gtcagtccga tggtctggcg tcggctgaga attgctgccg
      181 acacgtcact ggccgcgctt cacttcattt tccagatagt gcagggctgg ggggacgacc
      241 atctccatca atttcacatt tatggcaaag attacggtat ctcctacgaa ggaggcatcg
      301 gttttgtcga taatccgttc cgggtcgtga ttgatgattt tgcttttgat gctggcgatc
      361 gctttaccta tgaatacaat ttctttgagc actggcttca tgacattcgt gttgaggcca
      421 tttatgaaaa ctctacgctg aaagcgccgt tttgtataag cggccatggt atgcccggag
      481 ccacagctgc ggatgagttc gataaaaccc tggcgtttct tgaagctatc gttaatgcgg
      541 atgatgaaac aacggtcggc gagatccgcc cttttgctga cgacctggat gctgtcaggt
      601 tcaaccgcca caaaatcaac cggcaactga gcaggctcga ccttgcatct ccggtactgg
      661 agcctgaagt tatctggctg ggccgtcgtc gctgactgat gtctataaag gtatacgttt
      721 tcagcatgcc tctcagatcc ccgaaaaaat gtctataaac ctccctaaat cagtgtttat
      781 ggattattct ttaaatctct atgatgttta aagaccttcc ggaggttttg cgtgcaatat
      841 gcctacatca gggtaagctc ggccgatcag aatacggccc gtcaggaaga ggcattgtca
      901 aaggcgggtt ttcagcctga taaaatttgt gttgagcatg ccagcgcgaa ggacacaaac
      961 cgcccggggt tacaggaact cctggggcaa ttacgccctg gtgatacgct tctggttcat
     1021 tccatcgatc gcctttgccg gaatatgtcg gatatgtgcg ctgtcaccac ccggcttcgt
     1081 gaccagggcg ttacccttat ttttctgaaa gagcagctga cgttcagcgc cggcacaaac
     1141 aatccgatgc aggaactgca gctgcacatg atgtcggcat tcagccagtt tgaacgagcc
     1201 ttactgaagg aaaggcaggc cgacggcatc gccgcgaaaa aagcacgggg tgaaaaaaca
     1261 gggcgccccg gcgccgatat caaaaagatc caggaaattg acgcactcag aagcagaggg
     1321 gtcaggctca ggatcgcctg cgaccatgcg ggccttggcg tttccacata ttataagctt
     1381 cgtcaccaga tgaacaacca gtagccggga gatagtcatc caccacagca ggaggcaatg
     1441 atgcaactga cgctccagat agtcatcacc gatgaatcag gctccagtcg aacggaggaa
     1501 ctgatgacaa tccagaaatc aggggagacc cggaacgaca tcggattatc ggtgtcagaa
     1561 tccaagctgt tgctgaatac ggtccagcag tcggtggtcc agctgcaggc agacgaatat
     1621 actcaacacc atatccggtg ccctcactgc cttgctgcgc gcagaatcaa aggcaaacag
     1681 aaaatacggt accggacgct gtttggcgtt attccggtat ccgggcttcg ggtgtaccgg
     1741 tgccgttgtg aagagagtga tacaaagaca gtcagtttgc tcagcgactg ggccggcgat
     1801 tattctcacc cggcactgaa atatattgaa acccgctggg cctcgatgat ctcctatgaa
     1861 atgacgactc gtctgctgaa agatattctg ccggtgggcc acagcctgaa tgcctcaacg
     1921 gtgaggaatc atttatgcca ggtggcgcag cgtcttgacg ctgaggctga agctcattca
     1981 ggttttcttt ccggctgccc ccgcgactgg gggaacctgc ccaggccagg aaaaccgctt
     2041 gtggtgggta tagatggtgg ttatgttcgc gatcgggatg acaaaaagcg taattttgaa
     2101 attattgccg ggaagtcttt ttccgttggt gcgcctgctg acacccgccg cttcggtttt
     2161 gttcagaagg atgactgtca tccggaacgc aggctcatga cccacctttc agcgcaggga
     2221 atgcaggcca accagcagat attttttctg tccgacggcg cggataatct cagggacctc
     2281 cagttcggta tgtaccctga gtcaacgcat gtgctggact ggtttcatat caccatgagg
     2341 ctgaaggtgc tcatgcaata tgcccgggga ctgctggtat cagatccaga ggcggggagt
     2401 aaagttctgg cactgctgga aagcattaaa cgatatctct ggcatggcaa tgttgtcgct
     2461 gcgctggaac atattgataa ttgcgttatg tattgcgatg accctgaact cagctatcct
     2521 agcctgaaat ccctgcaaaa acatctggat gaaatgtata cctacatccg gaataataag
     2581 atgatgatcc caaactacgg cgaaatgcgt cggtacggag agccggtatc aaccgcgttt
     2641 gtggaatcca cgatcaatga agtgatcgcc agacgaatgg ccaaaaagca gcagatgcag
     2701 tggagcagaa aaggggctca ttatttgtta cagacccgga ccgccgtttt gaataacgaa
     2761 ctgcaggata aatttgtctg ctggtatccg ggttttcaaa gtgacgggaa gggaccagcg
     2821 atggcagcat agctcacgct gccccaatat tttttatgct ctcggtcgta tggaagctgg
     2881 accgcctcgg tcgtgatctt cgtcatctca ttaatacagt gcacgacctg accgcgaggg
     2941 ggaccggtct gaaagtgctg accggtcacg gggcgactat cgacacaaca acggccgctg
     3001 gcaagcttgt cttcggtatc tttgccgcac tggcggagtt tgagcgcgaa ctgattgctg
     3061 aaagaacgac tgcaggtctg gcatcagcaa gagcccgtgg gcggaacggc ggccgacctt
     3121 ataaaatgac gccagtcaaa ctgcgactgg caatggcatc aatgggacaa tcagaaacaa
     3181 aggtcagtac gctatgccag gaacttggga taacccggca aactttgtac aggcatattt
     3241 cccctgttgg tcaactgcgg gcggacggga tcaagctgct caaccgtgga taatttgaac
     3301 ggacggaaaa atctggggtt ccggcgtagt acccctatca cctccacatt tttgcaacag
     3361 tgccttataa atggcagcct tatgtttact tgccaagatg ggctaccata tgatgtttta
     3421 tactgagcaa tattaatatt atctttaatg gaaaaattaa gcccactaag tgacccactg
     3481 ctatatggct ctattgtgaa gacgcctgag ggtaaaaatt tatacatggt tgtccctatg
     3541 tctattattg caaggttgac aatattattc gttttataaa tttgagcgtt taaaatcaca
     3601 catcaaagca attagtcagg ataaacaaca atacccagtg cttcgagaat cagttcttgc
     3661 tgccaggctg caattttgat acctgatgta tcgactttgc ggggatctaa accgtcgagt
     3721 tcggcgtggc ataaattggc accctgtagg ctaaattgcc cccagacatc ttcggaaaaa
     3781 acacctcgac ttaagtctga ctctttcagt gatgcacccg ctaggttcgt tcctatccag
     3841 cgattttcaa acaactcaca tttttctaaa caaaccctct ccatattggc ataggaaaga
     3901 ttacatccag aaataaatgc tgagcaaaag tacatacgat tactcacttg atgggcaaag
     3961 tttgttcggg aaaagttggc accttttaaa tcacacgcac ggaactctat accgtagcaa
     4021 ttggcattac tgaagtttgc catcgcaagt tggcattgtt ggaaacttgc atcacgaaga
     4081 tctgcgacat caaagtggca gccttcgata tcaccctgtt caatgaactt gcagttgacg
     4141 aatgtcgtat cacgcaagtt agcacgtcga aagtcgctgc gaatgaatgt gcaagcggtg
     4201 aaggtgagat cacttaagtc tttatgtgaa aagttgtggt gccgatatgt atgattgtag
     4261 gtttccatga gtactccttg ttgaaaggag ggctagacta ccattataca tatgccaaga
     4321 cattaaaata aaagcctgtt acatcaacac attacaaccc ctcaatttac ctgttttaag
     4381 tggtcaaatt ggcgcgtaga gcgcctatcg ctcttgcatt ggcacgcggc cctgttgaaa
     4441 aaagttattc gctttagata gggttaggag agccgtgctt cgccggtaaa aaatcggact
     4501 atccgacgca gggcggactc atcggcggag aagcaaactt tgactatatc tccctacact
     4561 tggaagaggg ctagactggc cccatagatc accagacact tcgactctct taaaataaga
     4621 ggtagtcttg gaactatgtt taataccagt cagaacgata agatggttga agtgttatca
     4681 ggacctgagc ggcgtcggcg tcgtacaccg caggaaaaaa ttgccattat tcagcagact
     4741 atggagcccg gtatgaccgt gtctcatgtc gcgcgcctgc atggcatcaa tgcaaaccag
     4801 atattcaaat ggcgcaagca atattaagac ggttcactga cggctgttgc atcgggcgag
     4861 gaagtggtgc cggcctccga actcgctgcc gccaataagc aaattcgcga actccagcgc
     4921 cttctgggca aaaagacgat ggaagccgag atactcaaag aggccgtgga gttcggccgg
     4981 gcaaaaaaat ggattgcgca tgcgccctcg ttgcctggga acgacgaata agcgacgttt
     5041 gcagaagtct tggcgtgtcg cgtgcgcagt tgagcattcg ggttcaccgg ccatctgact
     5101 gacaggatcg cagacggcag ccccgttctg acgataccac cgtattgtcc cggataaaca
     5161 tggcggtggc tgacctgccg acgtatggtt atcgccgtgt gtgggcgctg ctgcgacggg
     5221 agtcggagcg ggacggcctg cctgtggtta atgcaaagcg ggtgtatcgc attatgagaa
     5281 cgcataatct gcttcttgaa cgtaaacccg ctgatccttg ccgtaagcgg gcccataagg
     5341 ggcgcgtagc agtagcggaa agtaaccggc gctggtgctc agatggcttt gagttccgct
     5401 gtgataatgg cgaaaagctg cgggtgacct tcgcgcagga ctgctgcgac agggagatca
     5461 ttgactgggc agcgagcacc ggaggctatg acaaagaaac ggtgcaggat gtcatgctgg
     5521 gtgcggtgga aaaacgcttc ggaaggcagt tgccaacagt gccggcgttc gcccggttgc
     5581 tgggactgga gccttgtacg acagcagtgc gaagcccgga aagcaatggc atagcagaaa
     5641 gcttcgtgat gacgataaaa cgggactaca tcagcatcag gattttaggg gtctgacgct
     5701 cagtggaacg aaaactcacg ttaagcaacg ttttctgcct ctgacgcctc ttttaatggt
     5761 ctcagatgtc ctttggtcac cagttctgcc agcgtgaagg aataatggcc gagcatattg
     5821 at
//
LOCUS       DADLDT020000192         5805 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000192 DADLDT020000000
VERSION     DADLDT020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.196"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JQH22_003912"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JQH22_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFB2208057.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JQH22_003913"
     CDS             578..1027
                     /locus_tag="JQH22_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFB2208058.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="JQH22_003914"
     CDS             complement(1028..1261)
                     /locus_tag="JQH22_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HFB2208059.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="JQH22_003915"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="JQH22_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFB2208060.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="JQH22_003916"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="JQH22_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFB2208061.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="JQH22_003917"
     CDS             complement(2212..3021)
                     /locus_tag="JQH22_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFB2208062.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQH22_003918"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQH22_003918"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFB2208063.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="JQH22_003919"
     CDS             complement(4677..5471)
                     /locus_tag="JQH22_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFB2208064.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JQH22_003920"
     CDS             complement(5461..>5805)
                     /locus_tag="JQH22_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFB2208065.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1523 a         1473 c         1286 g         1523 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLDT020000193         5784 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000193 DADLDT020000000
VERSION     DADLDT020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.197"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="JQH22_003921"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="JQH22_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFB2208066.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="JQH22_003922"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="JQH22_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFB2208067.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="JQH22_003923"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="JQH22_003923"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFB2208068.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="JQH22_003924"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="JQH22_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="JQH22_003925"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="JQH22_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFB2208069.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="JQH22_003926"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="JQH22_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFB2208070.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1305 c         1196 g         1640 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DADLDT020000194         5759 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000194 DADLDT020000000
VERSION     DADLDT020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.198"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..402)
                     /locus_tag="JQH22_003927"
     CDS             complement(<1..402)
                     /locus_tag="JQH22_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HFB2208071.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="JQH22_003928"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="JQH22_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFB2208072.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="JQH22_003929"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="JQH22_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFB2208073.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="JQH22_003930"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="JQH22_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFB2208074.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="JQH22_003931"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="JQH22_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFB2208075.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="JQH22_003932"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="JQH22_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFB2208076.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5759)
                     /locus_tag="JQH22_003933"
     CDS             complement(5558..>5759)
                     /locus_tag="JQH22_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFB2208077.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1342 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaaga
//
LOCUS       DADLDT020000195         5702 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000195 DADLDT020000000
VERSION     DADLDT020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.200"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..219)
                     /locus_tag="JQH22_003934"
     CDS             complement(<1..219)
                     /locus_tag="JQH22_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFB2208078.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="JQH22_003935"
     CDS             complement(216..1124)
                     /locus_tag="JQH22_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFB2208079.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="JQH22_003936"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="JQH22_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="JQH22_003937"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="JQH22_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="JQH22_003938"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="JQH22_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFB2208080.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="JQH22_003939"
     CDS             5533..>5702
                     /locus_tag="JQH22_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208081.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DADLDT020000196         5682 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000196 DADLDT020000000
VERSION     DADLDT020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.201"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="JQH22_003940"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="JQH22_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFB2208082.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="JQH22_003941"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="JQH22_003941"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HFB2208083.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="JQH22_003942"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="JQH22_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFB2208084.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="JQH22_003943"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="JQH22_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFB2208085.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1487 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gt
//
LOCUS       DADLDT020000197         5641 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000197 DADLDT020000000
VERSION     DADLDT020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.203"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            595..1773
                     /gene="ltrA"
                     /locus_tag="JQH22_003944"
     CDS             595..1773
                     /gene="ltrA"
                     /locus_tag="JQH22_003944"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFB2208086.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFSFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1856..2035
                     /locus_tag="JQH22_003945"
                     /pseudo
     CDS             1856..2035
                     /locus_tag="JQH22_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2065..2547)
                     /locus_tag="JQH22_003946"
                     /pseudo
     CDS             complement(2065..2547)
                     /locus_tag="JQH22_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2803..3783)
                     /locus_tag="JQH22_003947"
     CDS             complement(2803..3783)
                     /locus_tag="JQH22_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFB2208087.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3783..4982)
                     /locus_tag="JQH22_003948"
     CDS             complement(3783..4982)
                     /locus_tag="JQH22_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFB2208088.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5304..>5641)
                     /locus_tag="JQH22_003949"
     CDS             complement(5304..>5641)
                     /locus_tag="JQH22_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFB2208089.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1554 a         1284 c         1340 g         1463 t
ORIGIN      
        1 agcggtaaaa caaggactgc ccggctgtgg gtctacgttc gtgatgaccg taacgccggt
       61 tcggaaattg cagcataagt tatccatgct ctgaggtggg tggaggtggg ccctccggtc
      121 agggtttaca ggagcacaga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
      181 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      241 aagtaaactg tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc
      301 cgtcccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      361 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      421 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      481 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      541 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      601 gtcgcaacaa gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa
      661 cgtgatggca atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca
      721 ccagtgctgg aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca
      781 acagtatccg atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac
      841 ccgattttcc atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg
      901 aaacaatgtg ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg
      961 tttttcgacc atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct
     1021 aaatgggtca tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat
     1081 ggagaattaa taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt
     1141 gctaacttgt tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta
     1201 ccgtttgaga ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca
     1261 aggctaaaga acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg
     1321 aaaacgaata tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttctcc
     1381 tttctcggat atgacttcaa agtgcgtacg ctgaagaatt tcaaaggcga acggtaccga
     1441 aaatgcatgc cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag
     1501 tggcgtatac atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg
     1561 atagtgagag gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga
     1621 ctgtggagtg caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg
     1681 aaccggaagg ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt
     1741 cactggtatt tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc
     1801 acgtacggtt ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg
     1861 ttccgacagc gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct
     1921 gaacaatgtg gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc
     1981 ggcaaaccgg gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc
     2041 aatacggttc tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac
     2101 aatgccgtat tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga
     2161 aaccaaccga cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg
     2221 tacgggtcgt atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc
     2281 atttttctgg caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg
     2341 gtcgagtttc ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac
     2401 agtcatttta ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt
     2461 tcctgcgggt ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc
     2521 aatgccggtg attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat
     2581 ccatcatggc gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact
     2641 gttaaacggc taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat
     2701 ttcaccgtga aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag
     2761 ccaattaaaa actggtgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt
     2821 atcttttagt acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct
     2881 gccgaattca tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact
     2941 atcaaatttc cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga
     3001 agacatttcc ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt
     3061 gtcatcagca gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga
     3121 tatatcaccc agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt
     3181 cagctcagac tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag
     3241 ggcacgagtc accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat
     3301 accggcctct ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga
     3361 cgtctgcaaa tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac
     3421 aagtacacgt aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa
     3481 aatctcaata agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat
     3541 agttctggtg atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac
     3601 ctcttcaacc acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa
     3661 ttttgccttt cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt
     3721 attattcagg atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc
     3781 catcagtagt tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct
     3841 gctgccatac gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca
     3901 gatatcacgg tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa
     3961 acatcaagca tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct
     4021 gacttattcg ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca
     4081 tcctgttcaa taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg
     4141 aaatcaactt gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt
     4201 ttcaaaaatg cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc
     4261 agcttatcaa taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc
     4321 tcgcacagag tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca
     4381 taaacgccag ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg
     4441 ttctgcaaca tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc
     4501 agaaacatag tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct
     4561 gacagcaggt gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta
     4621 gacacaccgc cttttagact accgatgaat atggaatatg cctcactata acgatcgcga
     4681 tatttaggaa tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat
     4741 tttgtcgcgg ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg
     4801 actgcaccat ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa
     4861 tagacttgat aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt
     4921 agggcgagca acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc
     4981 attccccctc cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg
     5041 taatgcatgc aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta
     5101 ttaatcatcg gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga
     5161 tttcccccga ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca
     5221 gatggtgtat ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca
     5281 acaggcgggg gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg
     5341 actttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg
     5401 atgcgggtgc ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc
     5461 aggtgtttct ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg
     5521 ccgatacaga acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc
     5581 ccgaaagtgt aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt
     5641 t
//
LOCUS       DADLDT020000198         5601 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000198 DADLDT020000000
VERSION     DADLDT020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.204"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..411
                     /locus_tag="JQH22_003950"
     CDS             <1..411
                     /locus_tag="JQH22_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208090.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="JQH22_003951"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="JQH22_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="JQH22_003952"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="JQH22_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFB2208091.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1331 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tagtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggagg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DADLDT020000199         5582 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000199 DADLDT020000000
VERSION     DADLDT020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.205"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            28..243
                     /locus_tag="JQH22_003953"
     CDS             28..243
                     /locus_tag="JQH22_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFB2208092.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="JQH22_003954"
     CDS             236..622
                     /locus_tag="JQH22_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFB2208093.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="JQH22_003955"
     CDS             630..1316
                     /locus_tag="JQH22_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFB2208094.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="JQH22_003956"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="JQH22_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFB2208095.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="JQH22_003957"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="JQH22_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFB2208096.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="JQH22_003958"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="JQH22_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFB2208097.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4043..4414
                     /locus_tag="JQH22_003959"
     CDS             4043..4414
                     /locus_tag="JQH22_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208098.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4424..>5582)
                     /locus_tag="JQH22_003960"
     CDS             complement(4424..>5582)
                     /locus_tag="JQH22_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFB2208099.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1030 c         1215 g         1795 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 at
//
LOCUS       DADLDT020000200         5580 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000200 DADLDT020000000
VERSION     DADLDT020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.206"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..279)
                     /locus_tag="JQH22_003961"
     CDS             complement(1..279)
                     /locus_tag="JQH22_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208100.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="JQH22_003962"
     CDS             220..405
                     /locus_tag="JQH22_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208101.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="JQH22_003963"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="JQH22_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFB2208102.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="JQH22_003964"
     CDS             complement(1254..1670)
                     /locus_tag="JQH22_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208103.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="JQH22_003965"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="JQH22_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="JQH22_003966"
     CDS             1837..2877
                     /locus_tag="JQH22_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2208104.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="JQH22_003967"
     tRNA            complement(2905..2994)
                     /locus_tag="JQH22_003967"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="JQH22_003968"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="JQH22_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFB2208105.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="JQH22_003969"
     tRNA            3986..4061
                     /locus_tag="JQH22_003969"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="JQH22_003970"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="JQH22_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1504 a         1322 c         1292 g         1462 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DADLDT020000201         5575 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000201 DADLDT020000000
VERSION     DADLDT020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.207"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..290
                     /locus_tag="JQH22_003971"
     CDS             <1..290
                     /locus_tag="JQH22_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFB2208106.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="JQH22_003972"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="JQH22_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFB2208107.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
BASE COUNT         1255 a         1428 c         1901 g          991 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaac
//
LOCUS       DADLDT020000202         5559 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000202 DADLDT020000000
VERSION     DADLDT020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.208"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..641
                     /locus_tag="JQH22_003973"
     CDS             <1..641
                     /locus_tag="JQH22_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFB2208108.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQH22_003974"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQH22_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFB2208109.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="JQH22_003975"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="JQH22_003975"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFB2208110.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DADLDT020000203         5556 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000203 DADLDT020000000
VERSION     DADLDT020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.209"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(30..398)
                     /locus_tag="JQH22_003976"
                     /pseudo
     CDS             complement(30..398)
                     /locus_tag="JQH22_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            923..1897
                     /gene="hemB"
                     /locus_tag="JQH22_003977"
     CDS             923..1897
                     /gene="hemB"
                     /locus_tag="JQH22_003977"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFB2208111.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2003..2854)
                     /gene="tauD"
                     /locus_tag="JQH22_003978"
     CDS             complement(2003..2854)
                     /gene="tauD"
                     /locus_tag="JQH22_003978"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFB2208112.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2851..3678)
                     /gene="tauC"
                     /locus_tag="JQH22_003979"
     CDS             complement(2851..3678)
                     /gene="tauC"
                     /locus_tag="JQH22_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFB2208113.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3675..4442)
                     /gene="tauB"
                     /locus_tag="JQH22_003980"
     CDS             complement(3675..4442)
                     /gene="tauB"
                     /locus_tag="JQH22_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFB2208114.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4455..5381)
                     /gene="tauA"
                     /locus_tag="JQH22_003981"
     CDS             complement(4455..5381)
                     /gene="tauA"
                     /locus_tag="JQH22_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2208115.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1350 a         1513 c         1397 g         1296 t
ORIGIN      
        1 gttgtctgga gattcagggg gccagtctaa tcagttgtca tgcactcgga ataaatcata
       61 ccgtgaatag tgctgttttt gattgtcaga ctattggcgt tggtattggt agaaataccg
      121 tcgtccagat agtaggtaga aatcacgccg ttaacggtag cgttattaat gaccgggtag
      181 atatcgccgt tgtaatagct atcagcggca tagttgttcc agcctacata accatcatag
      241 taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta agtttgacca
      301 gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca agccaatgct
      361 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
      421 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
      481 ttatgctgcg ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca
      541 tatataattt atggagaaaa acacgcatca aattgataaa aggaagatat ttcagtatgt
      601 tgcagataat gttatttttg atgcgtacgc gttataaata tcaaaaacac aaaccagata
      661 aatatcttat caatatgcaa caaagtttga attattcaat ttatagcagc acaaccaaac
      721 gccaaaaaaa taataaccaa gagctataaa taaagattaa aaatattttc agtgaatatg
      781 aaattattgc tatttctatt acaaagatgt cctggtctct ctttcattct cttcttgtca
      841 acatcgcgac aactttcgta aaacactcct gccccgcttc aggtatactg tgcaccttaa
      901 ttccacaaac atcaggcaga ccatgacaga cttaatccaa cgccctcgtc gcctgcgcaa
      961 atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt agccttaacg acctggtgtt
     1021 gccgatcttt gttgaagaag aaattgacga ctacaaagcc gttgaagcca tgccaggcgt
     1081 gatgcgcatt ccagagaaac atctggcacg cgaaattgaa cgcatcgcca acgccggtat
     1141 ccgttccgta atgacttttg gcatctctca ccataccgat gaaaccggca gcgacgcctg
     1201 gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc aagcagactg tgccagaaat
     1261 gatcgtcatg tcagacactt gtttctgtga atacacttct cacggtcact gcggtgtgct
     1321 gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc aagccgtggt
     1381 tgcagctgct gcaggcgcag acttcatcgc cccttccgcc gcgatggacg gccaggtaca
     1441 ggcgattcgt caggcgctgg acgctgcagg ctttaaagat acggcgatta tgtcgtattc
     1501 gaccaagttc gcctcttcct tttatggccc gttccgtgaa gctgcaggaa gcgcattaaa
     1561 aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt cgcgaggcga ttcgtgagtc
     1621 actgctggat gaagcccagg gcgcagactg cctgatggtt aaacctgctg gagcgtacct
     1681 cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt atcaggtgag
     1741 cggtgagtac gcgatgatta agttcgccgc gctggcgggt gctatagatg aagagaaagt
     1801 cgtgctcgaa agcttaggtt caattaagcg tgcgggtgcg gatctgattt tcagctactt
     1861 tgcgctggat ttggctgaga agaagattct gcgttaattt ttttcctctc agaaaggggg
     1921 agttaacctg ttgaaagtac agtgaacgcc ccctcaccct aaccctctcc ccaagggggc
     1981 gagggaaccg tccgcactcg tatcaccccg cccgataaaa cggtttatcc ccaaggatcg
     2041 tcgcccgatg cataatccgc cgctgtggca ggtaatcggc attggcatag tgctgagtca
     2101 cgcggttatc ccaaatcgca atatcattcg gttgccagcg ccaacgcacc tgaaactccg
     2161 gtttagtgat atgggcgaat aaaaagccta acaaggcttc gctctctttc tcgctgacat
     2221 cgacaattcg cgtagtaaag ccttcattca caaacagcgc ctgtttaccg ctcaccggat
     2281 gcgttcgcac caccggatgt agcaacggag gatttttcgc gaccgcctcg cgccagcgtt
     2341 gatgttcctc ctcggttttg cggtatttgt attccgggaa tgatttacgg aaatcatgct
     2401 ccgcacgcag ccagctcagc agctggcgga agggaacaga gagcgcctca taggccgcaa
     2461 taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa ctctttagct gccagaatcg
     2521 cccctgcggg tggcgtttca ataaatgtca catcggtatg ccagttgtcg ttatccggcg
     2581 gattatcgtt atgggtatcc agcacgatga tctcgtcaac cccttcggca tgagggtaaa
     2641 cagggtgaat atgcagttcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgtaa
     2701 tagcttgatc gcgcagaaat accacctgat ggcgcagcac cgcatggtaa agctgttcaa
     2761 actgattatc gcttaacggg cgcgtcaggt cggcacccga aatttgtgcg ccgatatacg
     2821 gccccagcgg ggtaatgctc agacgttcac tcattgtact tctccatgcc agggcgtcag
     2881 acggcgctgt aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc
     2941 gatccccgcc agtaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc
     3001 taaaccgcgt gtcgcggcaa tcagctccgc cgctaccagc gtagaccagc ccacacccag
     3061 accaatgcgt aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag
     3121 cacctgcgca cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct
     3181 tttcacccct gccagcgccg acatcgccac cggcgcaaaa atcgctaaat agatcagtaa
     3241 gatcttcgag gtttcaccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg
     3301 tggcaccgga cgataaagtt cgattaccgg atccagaatg ccgcgcaccg tcgggcttag
     3361 ccccatggcg atcccgaccg gaataccgat caccaccgcc gcgagtagcg ccagcacaat
     3421 gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggtcc
     3481 ggcaatggtg agcagttttg ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat
     3541 cagttgcagc gccgccaccg tccaccatac agtgagtaaa accgctaacg tgccaatgct
     3601 taaggtcacc tgacgcgaga gcggccagcg ccatttcagc cgccgcgaat gcagtttttc
     3661 attaatgagc acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat
     3721 tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca
     3781 aagcggcgag caaagttgag ccgcagccgc tccagcacac ggccagggcc ggatgaaagc
     3841 agaaccagtt cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc
     3901 tgcttgcccg tctcctgcca gagtttcagc agcagagttt gcatctggtc gcgggtgaag
     3961 gcgtccagcg caccaaacgg ttcgtcgagt aataacagct ggggattcgc cgccagcgcg
     4021 cgggcaatcc ccacccgctg acgttgacca tcggaaagct gccagatata gcgtttttct
     4081 gcgccttcca gccccacttt tttcagcatc tggtgcgcga tttccagtcg ctgcattttc
     4141 tctatacctg ccaattgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtagc
     4201 agcccttcat tctgaaaaac tacgccacgc tctgctcccg gtccctcaat acgcttaccc
     4261 gccagttgaa tgctgccatg ctgataaggc acaaaaccgg caatcagatt cagcagggtg
     4321 gttttaccgc agccggacgg ccccagcacc accagtagct cgccgctttc cagcgtcagg
     4381 ttgatatctt ccagtgccgg tttgccgcca taatcggcat agagatgaga gatttgcagc
     4441 atctgcgcct ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct
     4501 acagccggga ccttgccctg ctctttcaaa aactgcgcgg tgtcgatgat tgctttgttc
     4561 accggtccgg tcagttctgc cgtttgttgc tgcggcgtca gataggtatt tcccttcacc
     4621 agccccggaa cgtcaccttc aggcacgccg cttaaacgcg ccagtttgct gatgttttcc
     4681 ggctgtttca gccacgcgtc agggttagca atgtacggtt gctgtgcatc gatggcgctt
     4741 ttggcgaacg ctttcacgac ctcaggatgt ttctcggcaa aatctttgcg caccacccag
     4801 acgtccagcg ttggcgcacc ccactgcccg acctgttcag aatcggtcag caccttgccg
     4861 tctttttcca gggcgttaac cgccggtgcc cagacataag caccatcaat atcaccccgc
     4921 tgccatgcgg cgataatcgc gggcggctgc aggttcacaa tctccactag ccctggttta
     4981 atcccccagt gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg
     5041 gcaatgcgtt taccaatcaa atcttcgggt ttgctgatag ttttctttac caccagcgct
     5101 tcggagttac ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggct
     5161 gcaaccgcta atgggctgga accgaggttg ccgatttgca cgtcgccaga agccagcgcc
     5221 cgcacgatgc tggctccgct gtcaaactta cgccagtcca cgctcgcccc gctctcttta
     5281 gcaaaggtgt tgtcggcctg agccactttc gccggttcgg ctgaggtttg atacgccacg
     5341 gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta
     5401 tatgactagg taatttattc aagataaccg ggaatgggta aaaaaagacg ggatttgatg
     5461 ggattcaata agttatgcgc cgattgcatt caagataaaa cgagttaaaa atgctcaatg
     5521 cttcgatatc gataaaacat tcaagatagg cgcgcc
//
LOCUS       DADLDT020000204         5546 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000204 DADLDT020000000
VERSION     DADLDT020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.210"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1234
                     /locus_tag="JQH22_003982"
     CDS             <1..1234
                     /locus_tag="JQH22_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFB2208116.1"
                     /translation="AAKAAMTVAALCEKHNVELVAIGNGTASRETERFYLDVQKQFPK
                     VTAQKVIVSETGASVYSASELAAQEFPDLDVSLRGAVSIARRLQDPLAELVKIDPKSI
                     GVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTRVAGLTRMMAQNIVAW
                     RDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDASTVHPEAYPVVERILA
                     ATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEKPGRDPRPEFKTAQFA
                     DGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISSLSNKFVEDPHTVVKA
                     GDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQNNRPAAKPRGREAQP
                     AGNSAMMDALAAAMGKKR"
     gene            1672..1899
                     /gene="feoA"
                     /locus_tag="JQH22_003983"
     CDS             1672..1899
                     /gene="feoA"
                     /locus_tag="JQH22_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFB2208117.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            1916..4237
                     /gene="feoB"
                     /locus_tag="JQH22_003984"
     CDS             1916..4237
                     /gene="feoB"
                     /locus_tag="JQH22_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFB2208118.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            4237..4473
                     /gene="feoC"
                     /locus_tag="JQH22_003985"
     CDS             4237..4473
                     /gene="feoC"
                     /locus_tag="JQH22_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFB2208119.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            4676..>5546
                     /gene="rpnA"
                     /locus_tag="JQH22_003986"
     CDS             4676..>5546
                     /gene="rpnA"
                     /locus_tag="JQH22_003986"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFB2208120.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT         1319 a         1450 c         1458 g         1319 t
ORIGIN      
        1 agccgcaaaa gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt
       61 ggcgatcggc aacggtacag cttcccgcga aactgaacgt ttctatctcg acgtgcagaa
      121 gcagttcccg aaagtgaccg cacagaaagt gatcgtcagc gaaactggcg cgtcggttta
      181 ctcagcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc
      241 ggtatctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa
      301 atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact
      361 ggatgcagta gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt
      421 tccgctatta acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg
      481 gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg
      541 gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct
      601 ggacgcctct accgttcatc cggaagccta tccggtggtg gaacgcattc tggcagcaac
      661 acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc
      721 tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga
      781 aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga
      841 gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gcagtgacca acgtcaccaa
      901 ctttggcgcg tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt
      961 atcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt
     1021 gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga
     1081 gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg
     1141 cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga
     1201 tgcgctggcg gcagcgatgg gtaaaaaacg ttaaacgccc ggactggccc ctaaacctgc
     1261 gcgataaggc gttcacgccg catctggcaa cgaacgcttt gcctgacata aaagtgccgg
     1321 agaatatctc cggcactttt attccacagc caaactcaca atatactccg ttaatattta
     1381 tcacttcatt aacaactgaa accttaatta aacattagcc agtccgggta actcactatt
     1441 cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta
     1501 ttcaaaccaa cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat
     1561 tgtgttgttg attatttaat ctctcctttg ttggcaaatc atctggtctc atgtcgcggt
     1621 caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac
     1681 actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc
     1741 caaaaactgc tttctctagg catgttacct ggctcctctt ttgatgtggt gcgcgtcgct
     1801 ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa
     1861 gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa
     1921 aaaattaacc attggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca
     1981 gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga
     2041 agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc
     2101 tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt
     2161 gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct
     2221 gtacctgacg ctgcaactgc tggaactcgg cattccctgc atcgtggcgc tgaacatgct
     2281 cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctatcgg cacgtcttgg
     2341 ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc
     2401 gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact
     2461 caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg
     2521 ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc
     2581 gtcgcagcat ctggatgcca ccctcgcccg tctgcgtaat gaaatggatg atccggcgct
     2641 gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac
     2701 cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt
     2761 cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat
     2821 cggcggggcg ttacagccgc tgtttaacgt cggctccgtg gcgctatttg tgcatggtat
     2881 tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg
     2941 cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct
     3001 gttcctctcc ttccttgagg actccggcta tatggcgcgt gcagcgtttg tgatggaccg
     3061 cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg
     3121 ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat
     3181 gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt
     3241 cgcggctgcc ttcttcgggc agaacggtgc cctggcggtt ttctcgctgt atatgctcgg
     3301 tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc
     3361 gacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat
     3421 ccagacctgg cagcgtctga agggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt
     3481 cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg tcgataacat
     3541 caacgactcg gcgctggctt ccgtcagccg ggtgataacc cctgtcttca agccgattgg
     3601 cgtacatgaa gataactggc aggcaacggt tggcctgttt acgggcgcaa tggcaaaaga
     3661 agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt
     3721 caatccggcg gaattcaacc tcggtgaaga gctgttcagt gcggtagatg aaacctggca
     3781 gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg
     3841 caacggtgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc
     3901 tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg
     3961 agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa
     4021 tatcgcttac tcactggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac
     4081 ttacagcctg gtgtgcattc tggcggtaat cctgtttaac atcgtagcta tcggtctgct
     4141 gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag
     4201 ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtacgt
     4261 gatttgctgg cgttacgggg ccgtatggaa gcgacccaga taagccagac attgaacact
     4321 ccgcagccaa tgattaacgc catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg
     4381 attcaggaag aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa
     4441 gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag
     4501 cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct
     4561 tgcagcgcaa cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactc gctaatgcac
     4621 agatgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag
     4681 taaaaagcag agttccaccc cacatgatgc gctgttcaaa ctctttttac gccaaccgga
     4741 gacggcacgc gattttcttg cgtttcattt accggcaccc attcacgcgc tctgtgatat
     4801 gaaaaccctc aagctggagt cgaggagctt tattgatgac gatctgcgtg aaagctattc
     4861 cgatgtgctg tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga
     4921 acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc
     4981 aatgcaaaat caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt
     5041 ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga
     5101 tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat
     5161 gcctgatgat gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca
     5221 tattcgtcaa cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg
     5281 atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa ctggcgacgc
     5341 tgtacgtttt aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag
     5401 tttaatgact attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat
     5461 agccaaaata atgcttgaat ccggagtccc tcttgcagac atcatgcgat ttaccgggct
     5521 gtcagaagaa gagttggctg cggcga
//
LOCUS       DADLDT020000205         5454 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000205 DADLDT020000000
VERSION     DADLDT020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.211"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="JQH22_003987"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="JQH22_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFB2208121.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="JQH22_003988"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="JQH22_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFB2208122.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="JQH22_003989"
     CDS             complement(1477..1881)
                     /locus_tag="JQH22_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFB2208123.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="JQH22_003990"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="JQH22_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFB2208124.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="JQH22_003991"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="JQH22_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFB2208125.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         1447 a         1339 c         1437 g         1231 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgc
//
LOCUS       DADLDT020000206         5400 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000206 DADLDT020000000
VERSION     DADLDT020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.212"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..497
                     /locus_tag="JQH22_003992"
     CDS             <1..497
                     /locus_tag="JQH22_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFB2208126.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="JQH22_003993"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="JQH22_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="JQH22_003994"
     CDS             2500..3114
                     /locus_tag="JQH22_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFB2208127.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="JQH22_003995"
     CDS             3114..3443
                     /locus_tag="JQH22_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFB2208128.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="JQH22_003996"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="JQH22_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HFB2208129.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="JQH22_003997"
     CDS             4530..4841
                     /locus_tag="JQH22_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFB2208130.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..4983
                     /locus_tag="JQH22_003998"
     CDS             4825..4983
                     /locus_tag="JQH22_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFB2208131.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4976..5374
                     /locus_tag="JQH22_003999"
     CDS             4976..5374
                     /locus_tag="JQH22_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2208132.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1181 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
//
LOCUS       DADLDT020000207         5350 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000207 DADLDT020000000
VERSION     DADLDT020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.213"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(75..557)
                     /locus_tag="JQH22_004000"
     CDS             complement(75..557)
                     /locus_tag="JQH22_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208133.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="JQH22_004001"
     CDS             complement(625..2574)
                     /locus_tag="JQH22_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFB2208134.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="JQH22_004002"
     CDS             complement(2659..2994)
                     /locus_tag="JQH22_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFB2208135.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="JQH22_004003"
     CDS             complement(2994..3350)
                     /locus_tag="JQH22_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFB2208136.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="JQH22_004004"
     CDS             complement(3350..4846)
                     /locus_tag="JQH22_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFB2208137.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="JQH22_004005"
     CDS             complement(4843..5004)
                     /locus_tag="JQH22_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFB2208138.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="JQH22_004006"
     CDS             complement(5014..>5350)
                     /locus_tag="JQH22_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208139.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DADLDT020000208         5337 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000208 DADLDT020000000
VERSION     DADLDT020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.214"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQH22_004007"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQH22_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFB2208140.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JQH22_004008"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JQH22_004008"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFB2208141.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JQH22_004009"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JQH22_004009"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HFB2208142.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JQH22_004010"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JQH22_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFB2208143.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1456 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLDT020000209         5163 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000209 DADLDT020000000
VERSION     DADLDT020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.215"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..221
                     /locus_tag="JQH22_004011"
     CDS             <1..221
                     /locus_tag="JQH22_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208144.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="JQH22_004012"
     CDS             complement(393..722)
                     /locus_tag="JQH22_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFB2208145.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="JQH22_004013"
     CDS             complement(727..912)
                     /locus_tag="JQH22_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFB2208146.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="JQH22_004014"
     CDS             complement(909..3548)
                     /locus_tag="JQH22_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFB2208147.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="JQH22_004015"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="JQH22_004015"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFB2208148.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5163
                     /gene="hslJ"
                     /locus_tag="JQH22_004016"
     CDS             4856..>5163
                     /gene="hslJ"
                     /locus_tag="JQH22_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFB2208149.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1305 a         1322 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taa
//
LOCUS       DADLDT020000210         5149 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000210 DADLDT020000000
VERSION     DADLDT020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.216"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            188..445
                     /locus_tag="JQH22_004017"
                     /pseudo
     CDS             188..445
                     /locus_tag="JQH22_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="JQH22_004018"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="JQH22_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFB2208150.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="JQH22_004019"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="JQH22_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFB2208151.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="JQH22_004020"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="JQH22_004020"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFB2208152.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="JQH22_004021"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="JQH22_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="JQH22_004022"
     CDS             complement(3603..4418)
                     /locus_tag="JQH22_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2208153.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5149
                     /locus_tag="JQH22_004023"
     CDS             4515..>5149
                     /locus_tag="JQH22_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFB2208154.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1173 c         1225 g         1376 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccct
//
LOCUS       DADLDT020000211         5023 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000211 DADLDT020000000
VERSION     DADLDT020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.217"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="JQH22_004024"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="JQH22_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFB2208155.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="JQH22_004025"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="JQH22_004025"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFB2208156.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="JQH22_004026"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="JQH22_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFB2208157.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="JQH22_004027"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="JQH22_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFB2208158.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="JQH22_004028"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="JQH22_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFB2208159.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1414 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DADLDT020000212         4998 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000212 DADLDT020000000
VERSION     DADLDT020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.218"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..239
                     /locus_tag="JQH22_004029"
     CDS             <1..239
                     /locus_tag="JQH22_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFB2208160.1"
                     /translation="LSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQA
                     QVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(440..1297)
                     /gene="sitD"
                     /locus_tag="JQH22_004030"
     CDS             complement(440..1297)
                     /gene="sitD"
                     /locus_tag="JQH22_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFB2208161.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="JQH22_004031"
     CDS             complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="JQH22_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFB2208162.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="JQH22_004032"
     CDS             complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="JQH22_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFB2208163.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="JQH22_004033"
     CDS             complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="JQH22_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFB2208164.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4362..>4998)
                     /locus_tag="JQH22_004034"
     CDS             complement(4362..>4998)
                     /locus_tag="JQH22_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFB2208165.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1332 a         1218 c         1197 g         1251 t
ORIGIN      
        1 gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat atggcgtgga
       61 gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa ctggcggcag
      121 caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag cgggagctga
      181 aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc gtggaataac
      241 agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa ctttcgatag
      301 tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc atgatagaat
      361 tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct ggcggacgat
      421 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gcgtttccct
      481 ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc gcgaataaga
      541 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca ccagtcattg
      601 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
      661 gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata cctactgctt
      721 tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc agccatctcg
      781 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
      841 attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg attatatcgc
      901 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttaa
      961 ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccataactg
     1021 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg cagaatactc
     1081 cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc acgatgccag
     1141 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
     1201 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
     1261 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg
     1321 cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg ctaaaagaaa
     1381 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
     1441 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga gcaggcgggg
     1501 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
     1561 aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
     1621 gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt cgaaaaaggt
     1681 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
     1741 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
     1801 gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
     1861 gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
     1921 gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
     1981 tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc
     2041 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg ccgagaccca
     2101 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tcttgcttcc
     2161 ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt
     2221 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc
     2281 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
     2341 gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag catcgttttg
     2401 ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg
     2461 ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc tctcgccaga
     2521 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg aaaatccacc
     2581 atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt ggctatacgc
     2641 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
     2701 gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt
     2761 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
     2821 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
     2881 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
     2941 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccggctttaa
     3001 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta ggtaccgggc
     3061 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
     3121 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
     3181 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
     3241 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
     3301 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
     3361 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt tggtaggttt
     3421 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
     3481 cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt ccttcggtga
     3541 tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
     3601 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
     3661 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
     3721 ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc gcgatgatgg
     3781 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
     3841 tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata atttagtacc
     3901 tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca taacctatac
     3961 aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta ggttgtttcc
     4021 tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg aaatgacttt
     4081 atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg caaaaaaggg
     4141 gcaaaaaagg ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt
     4201 aacatattga aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac
     4261 ttgttcaggt cgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt
     4321 ctctggtact aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc
     4381 aacatggcta tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc
     4441 atttgcgcag tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc
     4501 aacagccagc aggcaaaagt atgtcgcgtg tggtacggat tacggcggcg aataccagca
     4561 cgttttactg ctgcattcca tcttgtaccc aaactgctta ccgagtaata aggtttctgt
     4621 ttttcgttac acaccctggg catgaaaaca aaatgcagtt tttgtttttc agttctgccg
     4681 tactcccgat gataaaaagt gatttcgctt ttgcgatgat gcccggtcag tttgtattgc
     4741 tccttcagtg cttcaagagc cggctgcagt aatgttaccg tccggatccc ggcatttgtt
     4801 tttgggggac cgaacatatc aagtatcgtc aggtttcttc tgacattcac aattcccttc
     4861 tcgaaatcca catcctccca cgccagagct gccagttccc cgtgacgaag cccggagtaa
     4921 acggcaaatt tccacaagtt ttggctctgt cctttttcac tttccattaa tgcattgaat
     4981 tctgttttag ataacggg
//
LOCUS       DADLDT020000213         4970 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000213 DADLDT020000000
VERSION     DADLDT020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.219"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(183..767)
                     /gene="kptA"
                     /locus_tag="JQH22_004035"
     CDS             complement(183..767)
                     /gene="kptA"
                     /locus_tag="JQH22_004035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFB2208166.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1017..1700
                     /locus_tag="JQH22_004036"
     CDS             1017..1700
                     /locus_tag="JQH22_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HFB2208167.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1697..2158
                     /locus_tag="JQH22_004037"
     CDS             1697..2158
                     /locus_tag="JQH22_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFB2208168.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2171..3343
                     /gene="iadA"
                     /locus_tag="JQH22_004038"
     CDS             2171..3343
                     /gene="iadA"
                     /locus_tag="JQH22_004038"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFB2208169.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3408..4319
                     /gene="hypT"
                     /locus_tag="JQH22_004039"
                     /gene_synonym="qseD"
     CDS             3408..4319
                     /gene="hypT"
                     /locus_tag="JQH22_004039"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFB2208170.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEELL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="JQH22_004040"
     CDS             complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="JQH22_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFB2208171.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4701..4784)
                     /gene="idlP"
                     /locus_tag="JQH22_004041"
     CDS             complement(4701..4784)
                     /gene="idlP"
                     /locus_tag="JQH22_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFB2208172.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1174 a         1304 c         1240 g         1252 t
ORIGIN      
        1 tgaccggaag cgacgctgat tccagtcccc aactgaccgc ctgccgtaaa tcactttaat
       61 ttcagcagtt gttattaaag gatacccttt tacgagggta tcccttcaga ctgataacaa
      121 acgctacatt gccggatgtg ctgcgcttac caggcctata taacccctgc aatatattga
      181 atttattgtt tttgtacccc agtcggcata aacgaccact caagaaactc aacagcaacc
      241 gttgatgtca gccagacccc gttttccgct tgccagaaag ggatgcctcg tttcgccatt
      301 tcctgtgctt tgacggttaa gacaaccggc gatccgtgtc ttgctcccac tttgcgtgcc
      361 gttgcttcat cagcagagag atgcacgtaa tgacgatccc ctgcaatcag cccctgtttt
      421 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
      481 tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
      541 ccatcactgg aataactaaa acgcttttta tcgctggttg cgaccacggt atccagcaat
      601 gcacgggtta atcgcttgcc cgctttttgg gcacaaagta tgagcttatt gatatccgcc
      661 catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
      721 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc gttttcgtcc
      781 ctggattttc tgtttttatt tactaatgtc attgtcactg ggtttatgaa ttttctgcaa
      841 ctcaatcctg cgcagaacct tttccacaca gcgtaatttt tcccctatgc aaaaaacgca
      901 tagccagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttcagttc
      961 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
     1021 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
     1081 atctggcttt ttttctcacc ataatctttt ttcccggtgt tttttccgga acagacagct
     1141 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
     1201 atggagccac cacctcattt cgcggggtgg gtggcgcggg ggctaaagat ggctttctct
     1261 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
     1321 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
     1381 gcattccagg gatctgctcg ctggcactga tcgctaacct gcaaaacacg gatgccgccg
     1441 ccggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
     1501 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
     1561 cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
     1621 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
     1681 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
     1741 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
     1801 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
     1861 tgaacccgta atggcgctgt ggggattatc cggtgaagcg gcaaccgtcc ttttagccgc
     1921 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcaaccg ctgacgcatt
     1981 aaccggccac gatgtcaccg ttttactgcc cgcaatgtat ttaatgggca acccggtgca
     2041 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
     2101 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
     2161 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
     2221 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
     2281 cgttgccagc aatatccctt ctgacattgt accgaactgc acggttgtcg atctcagcgg
     2341 gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg gtggcggcga
     2401 agcaggtccc acgacgcgca cgccggaagt ggcgctaagt cgcctgacgg aagcgggcgt
     2461 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
     2521 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
     2581 tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcga ttatcgatcg
     2641 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
     2701 cctggctaat atggcggcag aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
     2761 caccgtgttc cacatgggcg acagtaaaaa ggcgttaaag cctatttatg acctgctgga
     2821 aaactgcgat gtgccgatca gcaagctgct gccgacccac gttaaccgca acgtaccgtt
     2881 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
     2941 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
     3001 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
     3061 gaatttaacc catatcggtg ttgcaggttt tgaaacgttg ctggaaaccg tgcaggtgct
     3121 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
     3181 cggtttcctt aacatgagcg ggaaaggcga aattctgcca ggcaatgacg cagacttact
     3241 ggtcatgacg ccagaattgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
     3301 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
     3361 taatggacgt agaatgcccc caagggcggc tgacagagta aaacgtaatg gatgactgtg
     3421 gtgcggtttt gcataatatt gaaaccaaat ggctttatga ttttctgact ctggaaaaat
     3481 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
     3541 gcatccgtgc actggaacag gcgattggcg ttgaattgtt taaccgccag gtgacgccac
     3601 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
     3661 tagaaagcaa ccttgcagag ctgcgtggcg gcggcgatta cgcgcaacgt aaaatcaaga
     3721 tagccgctgc acactctctt tccctcggtc tgttaccgtc cattatcagc cagatgccgc
     3781 cgctctttac ctgggcaatt gaagccattg atgtcgatga agcggtcgat aaactgcgtg
     3841 aagggcaaag tgactgtatt ttttcctttc acgacgaaga gttgctggaa gcgccgtttg
     3901 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
     3961 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
     4021 acatggtgcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
     4081 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
     4141 cctggctgcc ggagtatgcc atccaacaag aaattcgcag cgggcagctc gttgtgctga
     4201 atcgggacga acaggtgatc ccgattcagg cctacgcata ccggatgaat acccgcatga
     4261 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg cttagctgac
     4321 attctcaagc gtcgcactgc gccaaccgcc attatcccag accagatcaa acgttagcgg
     4381 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc agtacgcttc
     4441 tttcaaatta acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
     4501 ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt gccactcggg
     4561 catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa gagtgaggag
     4621 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
     4681 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
     4741 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
     4801 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
     4861 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatctga taaaaatcat
     4921 caaactcaaa aaaaaaacgc tagtatattc tgaatattcc gaatagttac
//
LOCUS       DADLDT020000214         4879 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000214 DADLDT020000000
VERSION     DADLDT020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.220"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..969
                     /locus_tag="JQH22_004042"
     CDS             <1..969
                     /locus_tag="JQH22_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFB2208173.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="JQH22_004043"
     CDS             1550..2773
                     /locus_tag="JQH22_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208174.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="JQH22_004044"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="JQH22_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFB2208175.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="JQH22_004045"
     CDS             3742..4272
                     /locus_tag="JQH22_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2208176.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4879)
                     /locus_tag="JQH22_004046"
                     /pseudo
     CDS             complement(4314..4879)
                     /locus_tag="JQH22_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1559 a         1002 c          908 g         1410 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aatacatgtg
     4861 tgcgtaaccc ggtaatgct
//
LOCUS       DADLDT020000215         4860 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000215 DADLDT020000000
VERSION     DADLDT020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.221"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..425
                     /locus_tag="JQH22_004047"
     CDS             <1..425
                     /locus_tag="JQH22_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2208177.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="JQH22_004048"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="JQH22_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFB2208178.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIEHDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="JQH22_004049"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="JQH22_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFB2208179.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="JQH22_004050"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="JQH22_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFB2208180.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4860
                     /locus_tag="JQH22_004051"
     CDS             4720..>4860
                     /locus_tag="JQH22_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFB2208181.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1389 a         1037 c         1078 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcacg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
//
LOCUS       DADLDT020000216         4822 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000216 DADLDT020000000
VERSION     DADLDT020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.222"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..95
                     /locus_tag="JQH22_004052"
     CDS             <1..95
                     /locus_tag="JQH22_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208182.1"
                     /translation="KGVKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            154..540
                     /locus_tag="JQH22_004053"
     CDS             154..540
                     /locus_tag="JQH22_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFB2208183.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            549..878
                     /locus_tag="JQH22_004054"
     CDS             549..878
                     /locus_tag="JQH22_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFB2208184.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            850..3588
                     /locus_tag="JQH22_004055"
     CDS             850..3588
                     /locus_tag="JQH22_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFB2208185.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            3588..3917
                     /locus_tag="JQH22_004056"
     CDS             3588..3917
                     /locus_tag="JQH22_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208186.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            3917..4615
                     /locus_tag="JQH22_004057"
     CDS             3917..4615
                     /locus_tag="JQH22_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFB2208187.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            4620..>4822
                     /locus_tag="JQH22_004058"
     CDS             4620..>4822
                     /locus_tag="JQH22_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFB2208188.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALV"
BASE COUNT         1166 a         1082 c         1594 g          980 t
ORIGIN      
        1 tgaaaggcgt gaagcagggc agtgtcagca tcgtgggcat gacctctgac ggagagtttg
       61 tggcagtggc tgcggtgacc gtcagcgcac cataacagga cgatactcat cattgccccg
      121 gttatccggg gctttttgca tccggagaac atgatgtttc tgaaacagga tacgtttaat
      181 tatgaaaaac agtccgtggt gctcagtgag ctgtccgggc tgcagagaat tgaatatctg
      241 acgtttgttc agcagcgaac ggcaaagttt gatgcacagg agggagaact gccggaggct
      301 gaacgacaga ttgcttttct gcgtatggga atggatatca atgcctggct ggtttcccgc
      361 tcactgtgga atgctgagca gtctcaggat gttgagacgc tttgcgcatc cattatgaca
      421 acatggtcgt atgatgcgct gggcgcgggg gcggagaggg ttctgtcgct gagcggtatg
      481 gggaccattg agaatgccgg ggatgatgat catgaggcgc tgacgccgga aaagtcctga
      541 cgcgggaaat gcagtttgtc atgcggcttg cccgggagtt ccggcgggca gactggcggc
      601 ggatgctgtc ggatatgtcg gccactgagc ttggtgagtg gggcgattat ttccggatgc
      661 agagcttcag tgatgtgtgg atggatgcgc agtttgcctc gctgaaggca ttgatcgtga
      721 gaatggtgtc cggcagcagc gatgctgcgg tggctgattt cagcctttta ccggaagaga
      781 acgggatacc ggagcgaacg gacgaagaac tgatgcatct tggggaaggt atttccggag
      841 gtgtgcgtta tggaccagat agccaacctg gtcattgatt tggggattga tgcggcagag
      901 tttaaaaatg aaattccccg tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa
      961 cggtcttctg cccgtatgca gcgttttatg gagcgtcaga ctcaggcggc ccggcagaca
     1021 atgcaggcgg cttcttcggc tgcaacagca gcatcagccc atgcgcagac ggtggagaag
     1081 aatgcacggg ctcatgaacg catggcccgt gaggtggaac aaacccgtct gcgcgtggat
     1141 gccctgaatc agaaaatgcg cgaggaacag gcgcaggcca gggcggctca gctgggggta
     1201 agcagtgctg cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg
     1261 ctggggctga aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg
     1321 cgcggcaatt ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg
     1381 acaggcgcac tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc
     1441 gcggcggtcc tgggtctggg taaagcctgg catgacggcc ggaaggaggg cgagaaattt
     1501 aaccgtcagc tggcgctgac gggacattat gccggtgtca ctgtcgggca gttgtggaaa
     1561 ctcagccggg ccatatccgg gaatggtatc acgcaacatg cggcagccgg tgcgctggca
     1621 caggtggtgg gcagtggggc atttcatgga aacgatattg gtatggtggc gaaagctgcc
     1681 gcacagatgg agcgatcggt tggccagtcg gtcagcgata ccataaatca gtttaagcgg
     1741 ctgaaggatg atcctgtaaa tgccgcgaag gcgctggaca atgcgctgca ttttttgaat
     1801 gccactcagc ttgagcagat acgcgtcctt ggggagcagg ggcggtccag tgatgcggcc
     1861 cggatcgcca tgtctgcgct ggcagaggaa accggtaaac gcacatctga tattgataat
     1921 aatctcaatg cgctgggtag tacgctgcaa accttgtctg actggtggaa gcagttctgg
     1981 gatgcggcca tgaatattgg tcgtgaagac tcgctggatg cgcagattga tgcgttacag
     2041 gaaaaaattc agcgcgcgaa aaaatatccg tggacaaacg cctccacaca ggtggagtac
     2101 gatcagcagc gtcttaacga tcttcaggag aaaaaacgcc ggaaggattt gcaggatgca
     2161 aaagcgcagg cagaacggaa ttaccaggag caacagaaac gccggaatgc tgaaaatgcc
     2221 gcgctgaacc ggatgaatga aacggaagct gcacgacatc agcgggaaat tgcgcgtatt
     2281 aatgccatgc agtacgccga ccaggctgtc agggatgcgg cgatacagcg tgaaaatgaa
     2341 cgttacgaaa aagccattaa gaaaaataca cgggcaaccc gtaatgatga ggccacccgg
     2401 ttattgctgc agtacagtca gcagcaggca caggtggaag ggcagattgc tgctgccaga
     2461 cagtcagcag gcattgccac tgaaaggatg actgaagcgc ataaacagct tctggccctg
     2521 cagcagcgca tcagcgacct ggacgggaaa aagctgacgg cagatgaaaa gagtgtgctg
     2581 gcccgtaaga atgagctgat tcaggcgctg acgctgctgg atgtgaaaca gcaggagctg
     2641 cagaaacaga cagcgcttaa cgacctgaga aaaaaaacgg ttcagctgac cagccagctg
     2701 gcagacaaag aacgtgcact gcgtgagcag cacaatctgg atattgccac tgcaggtatg
     2761 ggggataagc agcggcagcg ctaccaggca cagttgcgca tccggcagga ataccggcaa
     2821 cagttgcaac agcttgagaa tgacagtcgc cagaaaggca cttacgggac ggaggactac
     2881 cggagggctg aggaggtgct gaaggggagc ctgaagcgac aactgaatga aaacaaacgc
     2941 tactggcagg aactggaagt ggcgcagggc gactggaaaa acggtgccat gcgggcgttt
     3001 cagaatttta cggcggatgc ggataatgcg gcgggaacgg cagaacagat gtttacagtg
     3061 gcattcagca gtgccggtaa tgcactggcg acattctgta ccaccggtaa gctgaatttt
     3121 aaatccttca cctcttccct gttgtcagat atggccagaa ttatggcaca gatggccatg
     3181 atgcaggcgg taaagggcgt cggttcttta ttcggcttca cgactaatgc tgatggcggt
     3241 gtttaccagt ctgctgattt gagtcgctac agtggcacgg tggttaaccg tccgacgttt
     3301 tttgcttttg caaaaggcgc gggtgtgatg ggggaagcgg gacctgaagc cattctgcca
     3361 ctgcgtcgcg gtgctgatgg taagctgggg gttgtggcgg atactggtgg ttcaggcatg
     3421 gtgatgtttg ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata
     3481 ggtccggctg ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg
     3541 caacagcagg cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc
     3601 actggaaagt gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc
     3661 tgggcgatgg ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca
     3721 gcgtgacgat acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc
     3781 acggtggcgt ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggtgg
     3841 tttgtcgtaa atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg
     3901 aacaggtggt gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc
     3961 ggagcagtca gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg
     4021 ttattttttc tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca
     4081 gtatcaggca taccccattc aggggtcggg gtttgaactg aacggcaggg gctgtgctgc
     4141 ccgtccgaca ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct
     4201 gcagagtctg gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc
     4261 ggtgaacttc gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg
     4321 gcgcatcgag cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc
     4381 aacggagacg gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg
     4441 gacttaccgt tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa
     4501 accaacaacg gatatccgga aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg
     4561 caacaatacc ggtaatttcg gcggtttcct ttccatcaat aaactttctc agtaaatcca
     4621 tgacacagac agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt
     4681 gtggcttcgt ggtgagaacg ccggagggag acaggtatct tcccagcgag aatatctccg
     4741 gtgagccgga ggaacggttc cggatggctc cggaggactg gctgcgggca caaatgcagg
     4801 gtgagattgt ggcactggtc ca
//
LOCUS       DADLDT020000217         4800 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000217 DADLDT020000000
VERSION     DADLDT020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.223"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..527
                     /locus_tag="JQH22_004059"
     CDS             <1..527
                     /locus_tag="JQH22_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFB2208189.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="JQH22_004060"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="JQH22_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFB2208190.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="JQH22_004061"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="JQH22_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFB2208191.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="JQH22_004062"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="JQH22_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFB2208192.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="JQH22_004063"
     CDS             4170..4343
                     /locus_tag="JQH22_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208193.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="JQH22_004064"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="JQH22_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFB2208194.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4665..>4800)
                     /locus_tag="JQH22_004065"
     CDS             complement(4665..>4800)
                     /locus_tag="JQH22_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFB2208195.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT         1226 a         1103 c         1198 g         1273 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc agacctttta atttcaggct aattcttctg
     4741 ctgttgtagt attcaatata ttccgtaaca gcatccttca gttcgcttat attactgaac
//
LOCUS       DADLDT020000218         4747 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000218 DADLDT020000000
VERSION     DADLDT020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.224"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..262)
                     /locus_tag="JQH22_004066"
     CDS             complement(<1..262)
                     /locus_tag="JQH22_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFB2208196.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="JQH22_004067"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="JQH22_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFB2208197.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="JQH22_004068"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="JQH22_004068"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFB2208198.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="JQH22_004069"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="JQH22_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFB2208199.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="JQH22_004070"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="JQH22_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFB2208200.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         1225 a         1169 c         1136 g         1217 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctac
//
LOCUS       DADLDT020000219         4744 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000219 DADLDT020000000
VERSION     DADLDT020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.225"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..212
                     /locus_tag="JQH22_004071"
                     /pseudo
     CDS             1..212
                     /locus_tag="JQH22_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            355..576
                     /locus_tag="JQH22_004072"
                     /pseudo
     CDS             355..576
                     /locus_tag="JQH22_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(597..659)
                     /locus_tag="JQH22_004073"
                     /pseudo
     CDS             complement(597..659)
                     /locus_tag="JQH22_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1109..2521
                     /gene="ospC1"
                     /locus_tag="JQH22_004074"
     CDS             1109..2521
                     /gene="ospC1"
                     /locus_tag="JQH22_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFB2208201.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2625..2774)
                     /locus_tag="JQH22_004075"
                     /pseudo
     CDS             complement(2625..2774)
                     /locus_tag="JQH22_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2850..4547
                     /gene="ospD3"
                     /locus_tag="JQH22_004076"
     CDS             2850..4547
                     /gene="ospD3"
                     /locus_tag="JQH22_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFB2208202.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4664..>4744
                     /locus_tag="JQH22_004077"
     CDS             4664..>4744
                     /locus_tag="JQH22_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFB2208203.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRR"
BASE COUNT         1644 a          861 c          886 g         1353 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatgggggcg
      121 gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag ttacacagtt
      181 gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc gggaaaccgg
      241 tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc
      301 caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga ggaaatggac
      361 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact
      421 atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc
      481 gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc
      541 tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc gatctacgcc
      601 acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc ataagggaag
      661 taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct ctattaaaaa
      721 acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat ttcattgcag
      781 aatacacatt ccactaactg aaagcactcc ataagaggaa tcagagaaag tatcgttttt
      841 ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt atgaaaataa
      901 tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt ttgaaatatc
      961 tggcatcaca taacatcaaa agtatataat acccttatct aattacatgc agtatattgt
     1021 atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa gatattaatt
     1081 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
     1141 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
     1201 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
     1261 aaatattatt aaagatttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
     1321 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
     1381 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
     1441 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
     1501 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
     1561 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
     1621 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
     1681 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
     1741 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
     1801 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
     1861 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
     1921 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
     1981 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
     2041 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     2101 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     2161 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     2221 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     2281 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     2341 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     2401 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     2461 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     2521 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     2581 tattgtcagt atggctcaat gtgataatag ctggaaagtt tgatgggttt cgccccgttg
     2641 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     2701 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
     2761 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     2821 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     2881 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     2941 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     3001 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     3061 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     3121 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     3181 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     3241 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     3301 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     3361 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     3421 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     3481 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     3541 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     3601 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     3661 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     3721 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     3781 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     3841 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     3901 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     3961 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     4021 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     4081 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     4141 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     4201 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     4261 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     4321 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     4381 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     4441 tgggaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     4501 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     4561 gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt tacctccggt
     4621 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accggacctg
     4681 gatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg tggccggacg
     4741 tcgc
//
LOCUS       DADLDT020000220         4682 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000220 DADLDT020000000
VERSION     DADLDT020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.226"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            480..803
                     /locus_tag="JQH22_004078"
     CDS             480..803
                     /locus_tag="JQH22_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFB2208204.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPS
                     ISPTLLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKG
                     TDDDR"
     gene            742..1506
                     /locus_tag="JQH22_004079"
                     /pseudo
     CDS             742..1506
                     /locus_tag="JQH22_004079"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1481..1972
                     /locus_tag="JQH22_004080"
     CDS             1481..1972
                     /locus_tag="JQH22_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFB2208205.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKVAFSEHEQSVKAELSAS
                     ARRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILIVSALLIGTSGSILWWQGQQ
                     ITDNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRL
                     KQE"
     gene            1979..2194
                     /locus_tag="JQH22_004081"
     CDS             1979..2194
                     /locus_tag="JQH22_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFB2208206.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQREN
                     AVLNERVSDLSTQVLSLSEQLRRLSGN"
     gene            complement(2701..3819)
                     /locus_tag="JQH22_004082"
     CDS             complement(2701..3819)
                     /locus_tag="JQH22_004082"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFB2208207.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(3937..4026)
                     /locus_tag="JQH22_004083"
     CDS             complement(3937..4026)
                     /locus_tag="JQH22_004083"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208208.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(4462..4623)
                     /locus_tag="JQH22_004084"
     CDS             complement(4462..4623)
                     /locus_tag="JQH22_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFB2208209.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1258 a         1066 c         1233 g         1125 t
ORIGIN      
        1 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg
       61 gggcggagcc tatggaaaaa cggcagcgcc gcatcctttt tctgccccgc ttgtcatgcc
      121 gtatgccttt gctcttatcc gcgagcaggt aacgcccgcc gcagtcgaac ggcaggagcg
      181 gcagcgaccg agtgagcgag gaggcgtcat atcatcgtaa cctccatttt ctcctgtacg
      241 ccctgcagct gcattttccc ctgtcacatg aagcactttc attaagtgtt actccgccgt
      301 aacatattaa gccagtatac actccgctag cgctgcatga ctggttcagg gctgcgcccc
      361 gaaacccgct aaaagccact gacgcgcctg cggcttgtcc aaccccggcc gaccgggaaa
      421 ggttttcccg cccgtcccgg cgttatcagg agaccggttt gtcagagaaa cggaacaaga
      481 tgctcaccat gtgggtgacg gaggacgagc accggcggct gcttgagcgc tgcgacggca
      541 ggcagctggc agcgtggatg cggcagacct gcctggacga aaagcctgcg cgtaccggaa
      601 agcttccctc gatctcgccg acgctgctgc gccagcttgc cggcatgggc aacaacctga
      661 accagatagc ccgccgcgtt aacgccggcg gcggcaccgg ccatgacagg gtgcagattg
      721 ttgcggcgct gatggccatc gatgccggcc ttgagcggct gcggcacgcc gttcttgaga
      781 aaggaacgga cgatgatcgt taaatttcat ccacgtggtc gcggcggtgg cgccgggccg
      841 gtggactatc tgctgggtaa agaccggcag cgtgaaggcg ccagcgtcct gcaggggaag
      901 ccggaagaag tccgggagct gattgacgcg tcgccttacg tgaagaaata cacctccggc
      961 gtactgtcgt ttgcggaggc cgatctgcct cccggccagc gggaaaaact gatggccagc
     1021 ttcgagcggg tactgatgcc cggactcgat aaagatcagt acagcattct gtgggtggag
     1081 cacgcggaca aggggcgtct ggagctgaac tttctgatcc ccaataccga gctgctgacc
     1141 ggcaggcggc tccagccgta ctacgaccgc gccgaccggc cgcgcatcga tgcctggcag
     1201 accatcgtga acggcagact ggggctgcac gacccgaacg caccggaaaa ccgccgggcg
     1261 ctggtcacgc cgtccggact gccgaaaacg aagcaggagg ccgcagaatc catcacgcgc
     1321 ggactgctga accttgcttc gtccggggag ctgaaaaccc gtcaggacat cactgaggcg
     1381 ctggaacggt caggttttga ggtggtgcgc accacgaaaa gcagtatcag cattgccgac
     1441 ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgagcagtc ttttaacgct
     1501 ggcgaaggac ttagagcagc aatcgaaagc gcagaagcag agtaccggcg agatgctgaa
     1561 agtcgcattc agcgagcacg agcagtctgt caaagcggaa ctgagcgcaa gcgcgaggag
     1621 aatcagcgac gccatcctcg cccacgagca gagtatgagc gaggcgatgg agaaaaaccg
     1681 gcggagcgta ctgcggactg ccgggcggac atggctcacc atcctgattg tgtcagcgct
     1741 gctgatcggc acgagcggga gcatactgtg gtggcagggg cagcagataa ccgacaatta
     1801 cacgcatctt cgtcagcagg aagataccct ggcgaaaatg accgcccgga cgtggggagt
     1861 gcgctatcag gagagcagcg acggccggag gtttcttatc ctgccgccgg ggatgcaaac
     1921 cgaagccatc ccttacgacg ggacgacatg gatccgcctg aaacaggagt agcggaagat
     1981 gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca
     2041 aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg ggcttatgca
     2101 acgggagaac gcggtgctga acgagcgcgt gtcggacttg agcacgcagg tgctgagttt
     2161 aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca ccgagttccg gcagagggag
     2221 aatgagtatc agaaaacact ggagctggag cgccagccgc agaaaaccta ccgcgggccg
     2281 acactttaaa ccatccaggc agctgacaac ccgcacgaag aggggctctt acttctcccg
     2341 ctaaataggt tcgtgatcaa aaagatcacg gcctatacct gggggggaat tatgaaattg
     2401 cttaacgtca ttgaagctgt acttaactgc ctgtatctgg cggccgggct gtgggactgg
     2461 ctggagaagc atttacctgt ttttaaggcg ttagcgctga ttttagtgtg actttacacg
     2521 ccatgcatct ggctgcatgg ttatgcagtc gggtaaaatt tatctgtcgc gatcggggtg
     2581 gtttaccggg tgggttgttg ttggttttgg ctgactgccg ccccgttcgc ggcgaagctg
     2641 tccggggcgg tgcgggcaat gagatgttat ggtaaattca agtcttagat tttatgatgt
     2701 ttaattagct ttttttgcgt ttataatgtt catctttttt attaaaattg attcaatctt
     2761 tgatgtgggt attaatattt tctttgacaa atagaaagtt aaaaaaaaca ccattaatag
     2821 tataaataaa tacccaaaaa cagaagcgtt attaatttca attattgatg ttaatctgta
     2881 tattactaca tgctgaatca acatggatat gtagatgcat ttagcatatg agttgaaaaa
     2941 atagaggtta aatttcttat taattattaa tgctgctttg taaatgataa tgcttatgca
     3001 cattaaaggt attattgata actggtgatt gactatgttt gttttgaact cattaggccc
     3061 tattaataat actgtcaata tacatataac aaccgataga ataactgtgg tgtttttttg
     3121 taatttattt ttgtatttaa atgcaagcat cccaaccata aactcaggaa gtcggcaagc
     3181 aaaaaaccaa aatgggaagt ttaaaaatga tgatgagata tttgatattg ggaatgttgt
     3241 aattgttatc acaaataaaa aaaatatgtt acgtttaaat gtaaaggtat tcataacgtg
     3301 aaaaactatc ggtgatatca cgtacagcat aataacgcac ccaataaacc actcgcctgt
     3361 aaggaaaaga aaatcataag agaaatgggc gtttaatata ccatcaaggg caaaaaatac
     3421 tggaattatt ctagtgtact ggcctcctgt tgtcattatt gatacgagag ggactgtatt
     3481 aattatctct ttatttaaat aagacaataa aattaagagc aaagagataa aaaagtaagt
     3541 gataatataa ggagtaagga tccttactcc tttgtcaagg aaataatcaa tggtattctt
     3601 tctatctagt gaaataaatg ccaaatatcc agatataaaa aaaaacaagc tcacacctac
     3661 ctggccaaga acccctccat aaaatgatgg tatgaaagat agcctggttt ggctaaatat
     3721 gtagccataa tgagatataa taacaaaaat gcaggctgtt actcgcaatg tatcaattga
     3781 cggatctcta ttagacataa cctttatttc gctgttcaaa ttatcgcctt tagttgacga
     3841 tgtaaaatta acgctataaa taagctacac attctagagc agcgaaagga tgattcaagt
     3901 atttttaata ataaaacgca ttgataatgt ttatgttcag atggtgctga tagctttgtc
     3961 atgctgttcc actgatgttg ataacgacag agacttttgt ccttttcttg ttagatgctg
     4021 cctcagattc ggattatggc gttaaagggg tttgacgctc aaccgaacgg tttcacacgt
     4081 taagagcttt ttgtctgata aatcaaaata taatggacat tgaaaaacgc cctcaaaacg
     4141 gtgtcttttt aatcgaagat tggcacaaaa cggacaccac cgtccattag tcgatttttt
     4201 agacggcttt tctcgcctaa cgtgagtttt cgttcggttg agcatcagac cccttttata
     4261 ttgtagattt ctatttttag acagttggaa caggacaatt aacaacagat ctacttgaga
     4321 tcatttgctt gtcgtcgtag tctctttccc agaaacgaaa aaaccgccct gcaaggcggt
     4381 ttctcgaagg ttctcagagc gaccaactct ttgaaccaag gtaactggct tatgaggagc
     4441 gcagtaacca aatctgtcct ttcagtttag ccttaacagg cgcataactt caagactaac
     4501 tcctctaaat ccgttaccaa tggctaccgc cactggcgat ttgtcgtgtc tttccgggtt
     4561 ggactcaagt tgatagttac cggaacgggc gtggtagtcg gactgaacgg ggggttcgtg
     4621 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     4681 at
//
LOCUS       DADLDT020000221         4636 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000221 DADLDT020000000
VERSION     DADLDT020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.227"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..311
                     /locus_tag="JQH22_004085"
     CDS             <1..311
                     /locus_tag="JQH22_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFB2208210.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="JQH22_004086"
     CDS             468..1217
                     /locus_tag="JQH22_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFB2208211.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="JQH22_004087"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="JQH22_004087"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFB2208212.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="JQH22_004088"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="JQH22_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFB2208213.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="JQH22_004089"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="JQH22_004089"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFB2208214.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="JQH22_004090"
     CDS             4084..4557
                     /locus_tag="JQH22_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFB2208215.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DADLDT020000222         4550 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000222 DADLDT020000000
VERSION     DADLDT020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.228"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1224
                     /locus_tag="JQH22_004091"
     CDS             <1..1224
                     /locus_tag="JQH22_004091"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFB2208216.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="JQH22_004092"
     CDS             1224..1898
                     /locus_tag="JQH22_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2208217.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="JQH22_004093"
     CDS             2426..3187
                     /locus_tag="JQH22_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFB2208218.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="JQH22_004094"
     CDS             3231..4310
                     /locus_tag="JQH22_004094"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFB2208219.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="JQH22_004095"
     CDS             4307..>4550
                     /locus_tag="JQH22_004095"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFB2208220.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DADLDT020000223         4398 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000223 DADLDT020000000
VERSION     DADLDT020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.229"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            52..1265
                     /locus_tag="JQH22_004096"
     CDS             join(52..343,343..1265)
                     /locus_tag="JQH22_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFB2208221.1"
                     /translation="MTKNMRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTP
                     ETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPVEKIAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLK
                     PEIQRVYDENHSVYGVRKVWRQLLREEVTVARCTVERLMKVMGLAGVRRGKKVRTTVS
                     RKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSS
                     MKTTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKA
                     YYASIGNGDLAA"
     misc_feature    333..449
                     /locus_tag="JQH22_004096"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1461..3041)
                     /locus_tag="JQH22_004097"
     CDS             complement(1461..3041)
                     /locus_tag="JQH22_004097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208222.1"
                     /translation="MNNKYLNKYITSAIGLVAVFICFYISWMPVFHHGSWSGHDFLFH
                     INRVLSSVNGLKNGQLIPYYDVDYKNHPGYGSNLFYPPLANLLGSIFFYISHDFNVTV
                     KLLAIFIAISSFLTSYFVLIRINGDKLPSVIYSLCFSCSIYMVDNIFIRSSYPEAMAI
                     CAIPLFLYGLGYASNRESVFFLASANIIFILSNIPATLCSGVVFIIYYSINRRRLAQY
                     VVSVCISILLCSWYVFPLIYSTHGESFTMLDRDWFPTMSRTYISLYDLISGEIIKSGP
                     LKGMALGIGLPITFAFIWNFATDHNKKKQDYLLVLIILLIICGGINFGFLGGVFTHLS
                     KIQFTWRFIPFLLFSILLILHASGRIKLKYILLIFTSTCLMTSAITVPKKSSHNLTID
                     IAASANYKDYVLSNAPELGDRIKELKCDNGLSYAFSRTLGSNGLPVFTFSIPTKTTCS
                     IPFMSYKSLELDGAKKYSRDGYFKAELTKGNYIIRIDTTYRFKQVVLISFIVSICTIL
                     TILLFALKRKWKWKWKWK"
     gene            complement(3038..3964)
                     /locus_tag="JQH22_004098"
     CDS             complement(3038..3964)
                     /locus_tag="JQH22_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFB2208223.1"
                     /translation="MKISLVVPVFNEEATIPIFYKTVREFEELQQYDVEIVFINDGSK
                     DGTESIINSIASWDPLVVALSFTRNFGKEPALFAGLDHATGDAIIPIDVDLQDPIEVI
                     PHLITKWQAGADMVLAKRTDRSTDGRLKRKSAEWFYKLHNKISSPKIEENVGDFRLMS
                     RDVVENIKLMPERNLFMKGVLSWVGGKTDVVEYARAERFAGDSKFNGWKLWNLALEGI
                     TSFSTFPLRMWTYIGLFVAGISFFYGVWMIFDTFAFGNPVRGYPSLLVSILFLGGIQL
                     IGIGALGEYIGRIYVETKSRPKYILKKWKSQK"
     gene            complement(3961..4323)
                     /locus_tag="JQH22_004099"
     CDS             complement(3961..4323)
                     /locus_tag="JQH22_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFB2208224.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYGLSTSQALANFAGFVV
                     AVSFSFYANARFTFKASTSTLRYMLYVGFMGTLSAAVGWAADRCGLPPIITLIAFSAI
                     SLVCGFFYSKFIVFREAK"
BASE COUNT         1343 a          936 c          966 g         1153 t
ORIGIN      
        1 atcgccccgg gaatcctgga gactaaactc cttgagagag aggcaaacag gatgactaaa
       61 aatatgcgct tttcccctga ggtccgtcag cgggccgttc gtatggttat cgaaagccag
      121 aatgactatg actcgcagtg ggcggccatc agctccatcg cccctaaaat cggctgtacg
      181 cctgagaccc tgcgtacctg gctgcgtcag tatgagcgcg atgccggtgc aggtgacggc
      241 ggactgagca ccactgaacg ccagcgtctg aaggaactgg agcgtgaaaa ccgcgaactg
      301 cgtcgcagca acgatatcct gcgccaggct tccgcgtatt ttgcgaaggc ggagttcgac
      361 cgcctgtgga aaaaatagcg cctctggtgg ataccctgcg tggtacgcac ggagtcggac
      421 cggtatgcca cgagctggct gtcgccccgt caacgtatta ccggcatcgt gcgcatcaac
      481 ggcatccgga taaacgcagt cagcgggcac agcgtgatga cctgctgaag ccggagatac
      541 agcgcgtgta cgatgaaaac cacagcgtgt acggtgtgcg caaggtctgg cgccagttgt
      601 tgcgtgagga ggtgaccgtg gccaggtgca cagtagaacg gctgatgaag gtgatggggc
      661 ttgccggtgt gcgccggggt aaaaaggtcc gcaccacagt cagccggaaa gaagccgctg
      721 cgggtgaccg ggtgaaccgc cagttcgtgg cagaatgccc caaccagctg tgggtggctg
      781 acttcacgta tgtgagcacc tggcagggct tcgcttacgt ggcgttcatc atcgatgtgt
      841 tcgccggcgt cattgtgggc tggcgggtct catcgtcgat gaaaacgacg ttcgtgctgg
      901 atgcactgga gcaggcgctg tgggcccgtc gcccgtcggg caccatccat cactcagata
      961 aggggtcaca atatgtgtcg ctggcataca ctcagcggct taaggatgca gagttgctgg
     1021 cgtcaaccgg cagcacaggc gactcatatg acaacgcgat ggcggagagc atcaacggac
     1081 tttataaagc ggaggtgata catcgtaaga gctggaaaaa ccgcgccgaa gtggaactgg
     1141 ccacactgac gtgggtggac tggtacaata accgacggtt gctgggacga ctgggtcata
     1201 tcccgccggc agaagcggaa aaggcatatt acgcttccat cggaaatggt gatctggcag
     1261 cctgagttca cagacaaaac actctcccgg aaaacagggg cggttcattg cgatgtagag
     1321 ccatatgatt tgatcgacaa agacccgggc atgcaaatgt ccttgtcttt gtcgttatct
     1381 gacaggatca atcaccgcct ccccaggagg tggttttggg gtgacaacaa atatggtaaa
     1441 aaattaactt ttattgttaa tcatttccat ttccatttcc atttccattt cctttttaaa
     1501 gcaaataaca atatggtaag tattgtgcat attgatacta tgaaagatat taatacgacc
     1561 tgtttgaacc tataagttgt gtcaatacgt ataatataat tacctttagt caattccgct
     1621 ttgaaatagc cgtctctcga atattttttt gccccatcta attctaatga tttataagac
     1681 atgaaaggta tgctgcaagt ggtttttgtt ggaatactaa atgtgaaaac aggtaaccca
     1741 tttgaaccta aggttcgtga gaaagcgtat gacagcccat tatcgcattt taattcttta
     1801 atccggtcac caagctctgg tgcatttgat agaacataat ctttataatt tgcactggcg
     1861 gcaatatcaa ttgtgaggtt gtggctactt tttttaggta cagtaatcgc acttgtcatc
     1921 aaacatgttg atgtgaaaat taacaagata tattttaatt tgattcttcc tgatgcatgg
     1981 agaataagta aaatgctaaa tagcaggaat ggaataaaac gccaagtaaa ttgaattttt
     2041 gataaatgtg taaatacgcc acctagaaaa ccaaaattaa tgccaccaca aatgattagc
     2101 aatataatta aaacaagcag gtaatcttgt ttctttttat tgtgatctgt cgcaaagttc
     2161 catataaatg caaaggtaat tggcagtccg atccctaatg ccattccctt tagggggcct
     2221 gattttatta tttctccaga aataagatcg taaagtgata tgtatgttcg tgacattgtt
     2281 gggaaccagt cacgatctaa catagtaaat gactcaccat gcgttgaata tatcaatggg
     2341 aatacatacc aactacataa tagtatagat atacaaacgc taacaacata ctgagctaat
     2401 ctacgtctat ttatggagta atatatgata aatacaaccc cagaacataa agttgcaggg
     2461 atattggata aaataaaaat aatattcgca gaagctaaaa aaaatactga ttctctgttt
     2521 gatgcatatc ctagcccata taaaaataac ggaatggcgc atattgccat agcttctgga
     2581 taagatgatc ttatgaaaat attatcaacc atatagattg agcatgaaaa gcacaatgaa
     2641 taaattactg atggtaattt gtccccattt attctaatta aaacaaagta tgatgtaaga
     2701 aatgacgata tcgcaataaa tatcgctaat aacttaacgg taacattgaa atcgtgactt
     2761 atataaaaaa atatagaacc taataaattt gctaaaggtg gataaaataa attactccca
     2821 tacccagggt gatttttata gtcaacatca taatatggga ttaattgacc attctttaat
     2881 ccattgactg atgataaaac tctattaata tgaaataaaa aatcgtgacc tgaccagctt
     2941 ccatggtgaa agactggcat ccacgatata taaaagcaaa taaaaacagc aaccaagcca
     3001 atagcacttg taatgtattt gtttaagtat ttgttattca tttctgactt ttccattttt
     3061 ttagtatgta tttaggtctg gattttgttt caacatatat tcttcctata tactcgccaa
     3121 gggcgccaat gccaatcagt tgaatccctc ctagaaataa tatagatacc aaaagtgatg
     3181 ggtatccgcg aacagggtta ccgaaggcga atgtatcaaa aatcatccat acgccataaa
     3241 agaatgaaat acctgctaca aacaagccaa tatatgtcca catgcgtaat gggaaagttg
     3301 agaaacttgt tatgccttca agcgcaaggt tccatagttt ccatccattg aacttggaat
     3361 caccagcgaa gcgttctgct cgcgcatatt ctacaacatc agttttaccg ccaacccaag
     3421 acagaacgcc tttcataaag aggtttcttt caggcatcag tttaatattt tcaacaacgt
     3481 cacgtgacat taacctgaaa tcgccaacat tttcttctat ctttggtgag ctgattttat
     3541 tgtgaagctt gtaaaaccat tccgctgatt tacgctttaa tcgtccgtca gttgaacgat
     3601 ccgttcgttt agcaagaacc atatcagccc cagcctgcca tttggtaatg aggtgaggaa
     3661 ttacttcaat aggatcttgc aggtccacat caatcggaat gattgcatca ccggttgcat
     3721 ggtccagccc agcaaacaga gctggttcct tcccaaaatt ccgggtgaac gataatgcaa
     3781 ccacaagagg atcccaagag gcaatagaat ttataattga ctctgttcca tctttactgc
     3841 cgtcattgat gaatactatt tcaacatcat attgctgtaa ctcttcaaac tctcttaccg
     3901 ttttataaaa gatcggtatc gtggcttcct cattgaagac cggaacgacc agagaaattt
     3961 tcattttgct tccctaaaaa caatgaattt tgaatagaag aaaccgcaaa caagactgat
     4021 tgcggagaat gctattaacg tgatgatggg tggtaacccg cacctgtcag cagcccaacc
     4081 tacagcagcg ctcagggtac ccataaagcc aacatagagc atgtaacgta gcgtggaggt
     4141 ggatgcttta aatgtgaaac gtgcattggc ataaaagctg aaactcaccg ccaccacgaa
     4201 gccagcaaag ttcgctaacg cttggcttgt tgacaatcca taaatgcaaa caccaaacac
     4261 cacccagtga atgagtgtat tgagaacccc aatcgatgtg tacttagcaa agagttttaa
     4321 cattagaaaa atcagtaaat tctgaaaaag tgagagttta gcatcaacag tgaagtggtc
     4381 aacgaaaact ggccaccg
//
LOCUS       DADLDT020000224         4388 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000224 DADLDT020000000
VERSION     DADLDT020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.230"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            259..2256
                     /gene="betT"
                     /locus_tag="JQH22_004100"
     CDS             259..2256
                     /gene="betT"
                     /locus_tag="JQH22_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFB2208225.1"
                     /translation="MSENDTTPKKSTSQVNKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPIGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLAVLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWALLMGVVAIALLIAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDIIQPAMTLVQEELNKQG
                     TISHISDAAEDRIRLEVDLGNELNYIYEVRLRGYNSPTFALAALDNDEQQSEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2320..3597
                     /locus_tag="JQH22_004101"
     CDS             2320..3597
                     /locus_tag="JQH22_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFB2208226.1"
                     /translation="MISRWKWMLKQTFKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAKVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYVDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSTPVLYLSQGQESSISTDLLETIIVSDVRSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSAYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPGIFAEAAQTLAFETL
                     EHATRADHIDSDRYLIKSIYYNLFSGEDSNKKP"
     gene            complement(3686..4114)
                     /locus_tag="JQH22_004102"
     CDS             complement(3686..4114)
                     /locus_tag="JQH22_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HFB2208227.1"
                     /translation="MNGSCLCGTIEFELTHKPAVFYRCHCSLCRKQSGVGYNLATLVK
                     DSEFRWIKGENCIASWSKPTGYRTDFCNVCGSTVPNSLRDVPYVWVPVGLIDECLEME
                     CAGDFCTDDAMPWDETRSPSCHAGPVESLASLLKYLKLNS"
BASE COUNT         1090 a         1036 c         1059 g         1203 t
ORIGIN      
        1 aagagcagac gctggtaatt cgtgcctaat atacttcact taacttatat cccagctttg
       61 atacagatca tacaaattaa atgccccagt aattggcagg ctgagtaact taaaactcct
      121 tagaatatag gatgatttgt atcggcactc attccaccat gcattccgat taatcccgca
      181 tagccagttg aactttgcta ctctgtgaga ggtagtacct tctatccagt gcggatttaa
      241 ttaatggaat aaatgattat gagtgaaaat gatacaaccc caaagaagtc tacaagccag
      301 gttaacaaag cggtattctt tacatctgct ttgctaattt tccttcttgt cgcctttgcc
      361 gccgtattcc cggatgtcgc cgacaaaaat tttaaactac ttcagcaaca aatcttcacg
      421 aatgccagct ggttctacat acttgctgtg gccctgattt tactgagcgt cacgttcctt
      481 ggactctcac gttacggtga tataaagctg ggcccagacc atgcgcagcc tgatttcagc
      541 taccactcct ggttcgcgat gcttttttcg gcagggatgg ggatcggcct gatgttcttt
      601 ggcgttgccg aacctgtgat gcattatctt tcgccacctg tcggcactcc agagaccgtt
      661 gcggcagcga aggaagccat gcgcctgacg ttcttccact ggggtctgca cgcatgggca
      721 atttatgcca tcgtggcgct gattctggcc tttttcagtt accgtcacgg tctgccttta
      781 acgctgcgtt ctgcgctcta tcccattatc ggcgatcgca tttacgggcc tataggccac
      841 gcggttgata tcttcgccgt gataggcact gtctttggcg ttgcaacatc gctgggttac
      901 ggtgtattgc aggtgaatgc cggtttgaac catcttttcg gcgtgcccat caatgaaacg
      961 gtacaggtca ttttgatcgt ggtcatcacg gggttagcga cgatttcggt ggtgtccggc
     1021 ctggacaagg gaatacgcat cctgtctgag cttaatctgg gcctggcggt gttgctcctg
     1081 gcgctggtct tgtgcctggg tcccactgtg cttctgctga agtcatttgt ggaaaatacg
     1141 ggtggttatc tgtcggaact ggtgagtaaa acgttcaacc tttacgcgta tgaacccaag
     1201 tcgagcaact ggctgggggg ctggacatta ttatactggg gatggtggct ttcatggtcg
     1261 ccgtttgtag gtatgttcat tgcacgcgtt tcccgcggca ggacaattcg tgagttcgtg
     1321 actggcgtgt tgtttgttcc cgccggcttt acgctcatgt ggatgacggt gtttggaaac
     1381 agcgccatct atctcatcat gaaccagggc gccactgacc tcgccaatac cgttcagcag
     1441 gatgttgcgc ttgccctgtt taatttcctg gagcatttcc cgttctcttc cgtgctgtca
     1501 ttcattgcaa tggcgatggt catcgtcttc tttgtaacgt ctgctgattc gggggcaatg
     1561 gttgtggata ctctggcatc aggtggggtg gcaaacacgc ccgtctggca gcgaattttc
     1621 tgggcattgc tcatgggcgt tgttgctatt gccctgctga tcgcaggggg gctgagtgcg
     1681 ttgcaaaccg taacgatagc gagtgcattg cccttctcag ttatcttatt aatatccata
     1741 tacggactgt taaaagccct gcgtcgggat ttgaccaagc gtgaaagcct tagcatggcg
     1801 actattgctc ctacggctgc acgtaaccca attccctggc agagaaggtt acgcaatatc
     1861 gcgtatttgc cgaagcgctc tcttgtgaaa cgttttatgg acgacattat ccaacccgcc
     1921 atgacgctgg ttcaggagga acttaacaag cagggaacga taagccacat cagtgacgcc
     1981 gccgaggatc gtattcgcct tgaagtcgat ttgggcaacg agctgaatta tatatatgaa
     2041 gtgaggcttc gcgggtataa ctcaccgacc ttcgcgctcg ctgcgctgga taatgatgag
     2101 cagcagagtg aacaacatcg atattatcgc gctgaagtct atctcaaaga gggcggccaa
     2161 aattatgatg taatgggctg gaatcaggaa cagctgatta atgacatact ggaccagtac
     2221 gaaaaacacc tgcacttcct gcatctggtt cgttaacagc aacatgccgc cacagaggcg
     2281 gcatttattt atcccggctg caatatgagg gaatgcagaa tgatttcacg ctggaaatgg
     2341 atgctgaagc agacatttaa aaaactatgg ttcagggcaa cgttattcgc aattgtcgca
     2401 ataataacgg cccttttatc aattcttttt aaatcaatga tacctgagtc ggtttccgtg
     2461 aaggttggtg cggaagcagt cgataacatt ctgaacatac tggcatcgag tatgctggca
     2521 gtgaccacat tttcgctgag catcatggtc acagcctacg gttcagccac tactaatgtg
     2581 actcccagag ctactcgttt agttgttgaa gacgtcacca cacaaaatgt actggccacc
     2641 ttcatcggtt cttttctctt cagtctggta gggattattg ccctcaatat gggagcttat
     2701 ggagaaaggg ggagagtcat tttattcatt gtaacactgg ttgtcattgc cttaatcctc
     2761 atcacattgc ttcgctggat acagcatttg acctctctgg ggagggttgg tgagacaacg
     2821 gcaaaagtag aacaggcggc catcgaaaca tttattgcga gagcaagaaa tccctgtctc
     2881 ggcggatatc catggcttga gaacaatgaa cagccgaaag gaacggttgc agtttatccg
     2941 aaaaagattg gctatgttga atatgttgat atggtgaaac tcagcaagct gctgaccaat
     3001 gatccccgtc atgtatacct cgtggcgcag ccgggcagtt tcatacatcc gtccacgcca
     3061 gttttgtacc tgagtcaggg ccaggagtca tcaatcagca ccgatttact tgagacgatt
     3121 attgtctcgg atgtacgttc atttgctcag gacccccgat tttgtcttag cgtcatggcc
     3181 gaaatagcct gccgagccct ttcccccgca gtgaacgatc ctggaaccgc cattgatgtc
     3241 attggcagag gtgtacgtat actttccgct tacgcgcaga ataaatctga tgaaatagaa
     3301 gtgaaatatc cttctgtaca tgttgcacca cttcagaata acgatctact tgaagacttt
     3361 ttctcacctg ttgcgcgcga tggtgccagt atgagggaaa ttcagataag ggtccttaaa
     3421 ggactgtcga tgctgagtaa gggttggcct ggaatttttg ctgaagccgc acaaacccta
     3481 gcatttgaaa cattagagca tgctactcgt gctgaccata tagattctga cagatatcta
     3541 ataaaatcaa tttattataa tttatttagt ggcgaagatt ctaataaaaa accatagttg
     3601 cggagcaagc gatacggttg ttggcaagtc aagggcaaag cattcgcgtg agagtgggcg
     3661 tctgctttgt gccaaatgct gacgctcagg aattgagttt caggtacttt aaaagcgagg
     3721 ctagagactc gacaggtcca gcgtgacaac taggtgagcg ggtttcatcc cagggcattg
     3781 catcatcagt gcagaagtct cctgcacatt ccatttcaag acattcgtca attaaaccaa
     3841 caggaaccca gacataaggt acgtctcgca gactgttagg cacagttgag ccacatacat
     3901 tgcaaaaatc tgtacggtac cccgttggct tagaccatga agcaatgcag ttttcccctt
     3961 taatccagcg aaactcactg tctttgacta gtgtggcgag attgtaaccc acgccgctct
     4021 gctttctgca aagcgagcaa tgacaacgat aaaacactgc tggtttatga gtaagttcaa
     4081 attcaattgt tccacacaaa caagatccat tcatttcaaa accattccgg caaggcctaa
     4141 gagattttat gaaacattac catctttttt gtactgaata ggagcgctct ttttggcaac
     4201 tcaaaatcag ctatcagtcg gtctgaatat ccgctcttcg ctcttagcgg acattagcat
     4261 aggctattta ccataacgcc tcattacgcg taccgctcag acggcctgag cgcgtttctg
     4321 acgtatcccc gttaaaaaca gtaacaaacc acccgaaaat gcacgccaga aacgctacgt
     4381 aagaatct
//
LOCUS       DADLDT020000225         4327 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000225 DADLDT020000000
VERSION     DADLDT020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.231"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..904)
                     /locus_tag="JQH22_004103"
     CDS             complement(<1..904)
                     /locus_tag="JQH22_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFB2208228.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="JQH22_004104"
     CDS             964..1242
                     /locus_tag="JQH22_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208229.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQH22_004105"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQH22_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFB2208230.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQH22_004106"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQH22_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFB2208231.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="JQH22_004107"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="JQH22_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFB2208232.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="JQH22_004108"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="JQH22_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFB2208233.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..>4327
                     /locus_tag="JQH22_004109"
     CDS             4234..>4327
                     /locus_tag="JQH22_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFB2208234.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1274 a          924 c          926 g         1203 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggt
//
LOCUS       DADLDT020000226         4324 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000226 DADLDT020000000
VERSION     DADLDT020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.232"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..158)
                     /locus_tag="JQH22_004110"
     CDS             complement(<1..158)
                     /locus_tag="JQH22_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFB2208235.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JQH22_004111"
     CDS             complement(308..469)
                     /locus_tag="JQH22_004111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208236.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="JQH22_004112"
     CDS             complement(466..654)
                     /locus_tag="JQH22_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFB2208237.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JQH22_004113"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JQH22_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFB2208238.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="JQH22_004114"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="JQH22_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="JQH22_004115"
     tRNA            complement(2161..2237)
                     /locus_tag="JQH22_004115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JQH22_004116"
     tRNA            complement(2339..2414)
                     /locus_tag="JQH22_004116"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="JQH22_004117"
     CDS             complement(2559..3380)
                     /locus_tag="JQH22_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFB2208239.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="JQH22_004118"
     CDS             complement(3395..3751)
                     /locus_tag="JQH22_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFB2208240.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4324)
                     /locus_tag="JQH22_004119"
     CDS             complement(3764..>4324)
                     /locus_tag="JQH22_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFB2208241.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1178 c          856 g         1102 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc tctcccggta
     4201 cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc agcgaacgac
     4261 gtaataccgc ttccggtagc gcgtccgcca cctgattgcg gaccgcccac caggataatt
     4321 cagc
//
LOCUS       DADLDT020000227         4291 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000227 DADLDT020000000
VERSION     DADLDT020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.233"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="JQH22_004120"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="JQH22_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFB2208242.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..2265
                     /gene="ompC"
                     /locus_tag="JQH22_004121"
     CDS             1165..2265
                     /gene="ompC"
                     /locus_tag="JQH22_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFB2208243.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2429..2914
                     /locus_tag="JQH22_004122"
     CDS             2429..2914
                     /locus_tag="JQH22_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFB2208244.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3024..4013
                     /locus_tag="JQH22_004123"
     CDS             3024..4013
                     /locus_tag="JQH22_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFB2208245.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1329 a          880 c          817 g         1265 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
     1381 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
     1441 ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac
     1501 tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa
     1561 tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta
     1621 gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa
     1681 tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt
     1741 gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt
     1801 gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc
     1861 aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac
     1921 atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat
     1981 atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg
     2041 cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag
     2101 gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc
     2161 tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt
     2221 cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc
     2281 agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat
     2341 gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg
     2401 gacatcttac tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt
     2461 aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag
     2521 caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag
     2581 agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt
     2641 gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct
     2701 ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa
     2761 cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat
     2821 ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa
     2881 tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat
     2941 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
     3001 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
     3061 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
     3121 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
     3181 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
     3241 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
     3301 ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta
     3361 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
     3421 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
     3481 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
     3541 catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt
     3601 gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta
     3661 ataaaaatga actcttactg ctggatctat cccataataa actaacaagt ttacacaatg
     3721 tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa
     3781 tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat
     3841 atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata
     3901 ataaaataac ccacattgat actaataata catccgatat tggaacttta ttccccataa
     3961 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta
     4021 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
     4081 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt
     4141 ttcactatac tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
     4201 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac cttagggtaa tgctgccaac
     4261 ttactgattt agtgtatgat ggtgatttta a
//
LOCUS       DADLDT020000228         4183 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000228 DADLDT020000000
VERSION     DADLDT020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.234"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="JQH22_004124"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="JQH22_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFB2208246.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JQH22_004125"
     tRNA            complement(324..399)
                     /locus_tag="JQH22_004125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JQH22_004126"
     tRNA            complement(406..480)
                     /locus_tag="JQH22_004126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JQH22_004127"
     tRNA            complement(597..681)
                     /locus_tag="JQH22_004127"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JQH22_004128"
     tRNA            complement(690..765)
                     /locus_tag="JQH22_004128"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JQH22_004129"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JQH22_004129"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFB2208247.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH22_004130"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH22_004130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFB2208248.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH22_004131"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH22_004131"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFB2208249.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVCFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1039 c          928 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcaga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DADLDT020000229         4162 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000229 DADLDT020000000
VERSION     DADLDT020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.235"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="JQH22_004132"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="JQH22_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFB2208250.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..2409)
                     /gene="flhA"
                     /locus_tag="JQH22_004133"
                     /pseudo
     CDS             complement(332..2409)
                     /gene="flhA"
                     /locus_tag="JQH22_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="JQH22_004134"
                     /pseudo
     CDS             complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="JQH22_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3785..>4162
                     /locus_tag="JQH22_004135"
     CDS             3785..>4162
                     /locus_tag="JQH22_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFB2208251.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPF"
BASE COUNT         1037 a         1206 c         1019 g          900 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcggtta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacgtagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gccttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccac gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat tt
//
LOCUS       DADLDT020000230         4110 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000230 DADLDT020000000
VERSION     DADLDT020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.236"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(954..1145)
                     /locus_tag="JQH22_004136"
     CDS             complement(954..1145)
                     /locus_tag="JQH22_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFB2208252.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="JQH22_004137"
     CDS             1209..1532
                     /locus_tag="JQH22_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFB2208253.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="JQH22_004138"
     CDS             1522..1833
                     /locus_tag="JQH22_004138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208254.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="JQH22_004139"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="JQH22_004139"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="JQH22_004140"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="JQH22_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="JQH22_004141"
     CDS             2748..2981
                     /locus_tag="JQH22_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFB2208255.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="JQH22_004142"
     CDS             complement(3527..3994)
                     /locus_tag="JQH22_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208256.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1000 a          939 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagcccg tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DADLDT020000231         3971 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000231 DADLDT020000000
VERSION     DADLDT020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.237"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..356
                     /locus_tag="JQH22_004143"
     CDS             <1..356
                     /locus_tag="JQH22_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFB2208257.1"
                     /translation="FVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRFH
                     DLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNG
                     TNTAQEENVVYLNAR"
     gene            618..980
                     /locus_tag="JQH22_004144"
     CDS             618..980
                     /locus_tag="JQH22_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFB2208258.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            977..1897
                     /locus_tag="JQH22_004145"
     CDS             977..1897
                     /locus_tag="JQH22_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFB2208259.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1900..2851
                     /locus_tag="JQH22_004146"
                     /pseudo
     CDS             1900..2851
                     /locus_tag="JQH22_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2878..3118
                     /locus_tag="JQH22_004147"
                     /pseudo
     CDS             2878..3118
                     /locus_tag="JQH22_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3115..3420
                     /locus_tag="JQH22_004148"
     CDS             3115..3420
                     /locus_tag="JQH22_004148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208260.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3654..>3971)
                     /locus_tag="JQH22_004149"
     CDS             complement(3654..>3971)
                     /locus_tag="JQH22_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFB2208261.1"
                     /translation="ESEWKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNE
                     KKSALIAEATNVIAPLQDAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPE
                     SNL"
BASE COUNT         1149 a          778 c          767 g         1277 t
ORIGIN      
        1 tgtttgtcca caccaaacct gcctaccgaa gcgacggaat aaaaacagca gcggtaagga
       61 agatgagaac cgacagcaac aaggcatgga agggagcgtt aaagcgggca ggcattagca
      121 acttccgctt ccatgacctg aggcatacct gggcaagctg gctggttcag tccggtgtct
      181 ctcttcttgc gcttaaagag atgggaggat gggaaactct cgaaatggtt caaagatacg
      241 cccacctttc agccgggcat ctcaccgagc acgcgagcaa aatcgatgcg attataagtc
      301 gcaatggcac aaatacggca caagaggaga acgtagttta cttaaatgcg aggtaactta
      361 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaattata
      421 agaatccgct tgtaattcaa aggattatcc catcaacact gcgctcacac gtcccaccac
      481 atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtctc
      541 atcagacata gcaagtatcg atcaattgag acttggatga tagacttcat gcctttcaga
      601 gctcattgat taaataaatg ttaaagttat ttgtaaagta cacatctata ggtgtgctga
      661 acacacttat acactgggtg gtttttggtg tatgtatcta taccgcacat accagtcagg
      721 ctctggcaaa cttcacaggt ttcgtagtgg ctgtgagctt tagcttcttc gcgaatgcaa
      781 gattcacatt caaagcatcg actacagcga tgcgctacat gttatacgtg ggatttatgg
      841 gaatattgag tgtgattgtt ggttgggcag ctgataaatg ctcacttcct ccaatagtca
      901 ctcttatcac cttctccgcc atcagtctgg tgtgcggttt cgtctattca aagttcattg
      961 tctttaggga tgcgaaatga aaatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1021 gatacctgtt ttctataaaa cggtacgtga attccaagag ttgaagccat atgaagtaga
     1081 aattgtattc ataaatgacg gaagtaaaga tgccacagag tcaattatta acgcgctggc
     1141 tgtttcagac ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1201 cttatttgca gggttagacc atgcaagcgg cgatgctgta attcctattg atgtcgacct
     1261 gcaagaccca attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgagat
     1321 ggtgcttgct aaacgttcag accgctcaac tgatggacga ctgaaacgta agacagctga
     1381 gtggttctat aaattacaca acaaaataag caccccaaag atcgaggaaa atgtcggaga
     1441 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctgttgcctg agcgcaatct
     1501 tttcatgaaa ggcatactga gctgggtggg tggtcagacg gatgtcgttg aatatgtacg
     1561 cgcagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1621 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1681 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacaccc ttgtctttgg
     1741 taacccagta cgcgggtatc cctccctgct tgtatcaata cttttcttgg gtggagtgca
     1801 actgatcggg attggtgttc tcggtgaata tataggtaga atttacttag aaacaaaatc
     1861 aagacctaag tatattttga agaaaggtag aaaataacca tgagcatatg tattaaacaa
     1921 tcagcattaa aaatattatt ggctttatca gcactcctaa taacttggtt gactaccaga
     1981 tactttccag ttgaaccgga tgttgctaac agcccaattg tatggaggca tattttagag
     2041 aatggtatat catcaatcca cgactggaaa ccaaccgtgg ataattggta tttcaccgtt
     2101 tacccaatta actttttatt ttatatgctg ttaggtgatg atggcttagt agcattaaga
     2161 ttatcaactg cagttttctc tattgctata gtaatagcag cgatgttaac gctacgtaag
     2221 gcattcgggt ttacccctgc tttattttct attatacttc tatctctaat accatatttt
     2281 tcatacactt atggttttgt atcacatcct ttttctcata attcaacaaa cgctttcggc
     2341 tttttatgct tgcttatatc tgttttcaac atacagtata aaaatatatt tatcactctc
     2401 ctcttaagtt tgacagcatt attttccagc gtatccgatc cgtggtttac agctgcattt
     2461 tttattccac tattaatatc ttacttttta ttctctgtat gggataaaaa attatttaag
     2521 catactgcat taatactatt tgcatgttta atctctcttt ccaatgtatt gcaaaatcta
     2581 cttaacatac ctccccacca atttgaaatt gtatcactca atgatatgat tttaaatgca
     2641 aaatggtgca ttctgttaat aggaaaaagt cttaatttat tagttgtcga caataacgca
     2701 acatcttacg cctcatttgt catttggttc attgccatta ttacatctgc gtggtttgtt
     2761 ttatcggaca ataaaaaaaa cacataccgt atttatattg tgttattttc attattatct
     2821 atcgcaggta ttgtctcatc attcatactg agctataaat cccctgatta tattagcatg
     2881 cgtttcttta tgaatgttac ctgtttcgca ctgatattgt gctgtatcgg tacatcaaca
     2941 aaagcaaaga tattatttta tctaatcgca tttttatttt cgatcagttc aattaaatct
     3001 tacacaaata atgcttcacc attacacgat caggagaaga ttgttaaatc atacattgat
     3061 tttttaaaaa aaaataatct ccattatggg tacggctctt tttgggattt gtcaatgact
     3121 gtaaattggt tatctggtgg ggacatacag ataactcctg ttttctttaa tgctgattcg
     3181 gggaaaatta actttactgg cgtgagacaa cagacactgg cttcttggca tagcaaggag
     3241 gcgttcaatt ctgctcctga gcgacagttt atagcagttt ccatagctaa cgaacctgaa
     3301 cgatgtaaag aaatgacatc ctgccttgca ggtattcaag aacaattagg taagcctgac
     3361 gaagttttga actttgaagg aagagtaata ttagtattta acaaaaaact taacctatag
     3421 taaattgccc cctaccatat aaaccacact aacgaatgaa taacccgtct ttttcgacgg
     3481 gttatatccc tatcaggaat aaaaatggag tggttgccgt atacaacaaa ccctcattta
     3541 gagctatatt ctagagtata tgagatacta taaagttgaa ggagctatca ttagaaaaac
     3601 catatgctgt tataattaga tgtctcgctt ttcatccaat aacgaatttt ctcttataga
     3661 ttgctctctg gttttttcgg ccactctgta ttttttatat caacacgcat caatagtgcg
     3721 cgatattttt tccatgccag taacagtttc gtttcatcat ctgttgccat atctaaatca
     3781 accgcatcct gaagcggcgc tattacattc gttgcctctg ctatcagtgc agatttcttt
     3841 tcattattta ttaaggctaa ttgttctggt gttggagaag gacggtcaac aatgacaggg
     3901 tgaccattct caccacaggt aatcattttt gttacactct gcgaattaat cagtgttttc
     3961 cactctgatt c
//
LOCUS       DADLDT020000232         3790 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000232 DADLDT020000000
VERSION     DADLDT020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.238"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(452..824)
                     /locus_tag="JQH22_004150"
                     /pseudo
     CDS             complement(452..824)
                     /locus_tag="JQH22_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1068..1187
                     /locus_tag="JQH22_004151"
                     /pseudo
     CDS             1068..1187
                     /locus_tag="JQH22_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1437..2303
                     /gene="ospB"
                     /locus_tag="JQH22_004152"
     CDS             1437..2303
                     /gene="ospB"
                     /locus_tag="JQH22_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFB2208262.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2632..3372
                     /gene="phoN2"
                     /locus_tag="JQH22_004153"
     CDS             2632..3372
                     /gene="phoN2"
                     /locus_tag="JQH22_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFB2208263.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1161 a          745 c          761 g         1123 t
ORIGIN      
        1 ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg tttagtctcc
       61 aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta ctgttggcat
      121 taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg ttctcggggt
      181 atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg aaagcgctga
      241 tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc cagcactcta
      301 tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc agaaattaag
      361 gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat ttcttgatta
      421 tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg ttatcattgg
      481 cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc ggatgccatt
      541 cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg tcaggtttgg
      601 gcatgatact gatgtagtca tactttattg ttttcacgaa gctctttgct attctgttac
      661 tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat ccgggtgaac
      721 tggtgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt cactggagac
      781 gccggaagct cgttgccgaa gcggcgttcc accgctccca gcatgtcgcc aacgggagag
      841 catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag tacgctctga
      901 acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta aaacagcact
      961 tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta aagaggttgt
     1021 gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat catacttggc
     1081 gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa cattaccgga
     1141 acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga tgatctccac
     1201 ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt gtagttgaaa
     1261 ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc cagaatataa
     1321 ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca tactatatga
     1381 aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat gtaggtatga
     1441 atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa agcatatcag
     1501 atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact cacccaaaag
     1561 cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt ctatctatta
     1621 tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat gtttttaaaa
     1681 atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca cagagtgaac
     1741 caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt tctgtaaaaa
     1801 aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag gtttatattc
     1861 ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt attgatgtac
     1921 aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat atccgtttta
     1981 cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc cctgctgaaa
     2041 gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct ttgctagagc
     2101 agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa atatccggct
     2161 atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat tatcgttcaa
     2221 cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa aagactttta
     2281 ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt gtttgcattt
     2341 aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa aatcacaaat
     2401 catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca ttcttttacc
     2461 acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga ctattttttg
     2521 tttttccatc actctgttca aatttttccg catgacttgt gttttttgta atacagctcg
     2581 ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat tatgaaaacc
     2641 aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc agcaaatgct
     2701 ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc aatacttccg
     2761 ccgcctccgg cagaggattc agtagtattt caggctgaca aagctcatta tgaattcggc
     2821 cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata ctacgagaat
     2881 tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa taccccaatc
     2941 ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt gcgtaacgcc
     3001 aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac ctgtacacct
     3061 gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc atcctttggt
     3121 tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga aatacttcga
     3181 cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca aagcgatgta
     3241 gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac acctgaattt
     3301 accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc taccaatgaa
     3361 ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag acaaatctac
     3421 cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga atttaaggga
     3481 ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta ttatgctgcc
     3541 atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta ggaaactcgg
     3601 ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc accatcccct
     3661 atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct aaatatattt
     3721 taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa aaaatcaatc
     3781 atttttgaat
//
LOCUS       DADLDT020000233         3740 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000233 DADLDT020000000
VERSION     DADLDT020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.239"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..338)
                     /locus_tag="JQH22_004154"
     CDS             complement(<1..338)
                     /locus_tag="JQH22_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFB2208264.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKR"
     gene            complement(357..818)
                     /gene="tsaE"
                     /locus_tag="JQH22_004155"
     CDS             complement(357..818)
                     /gene="tsaE"
                     /locus_tag="JQH22_004155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFB2208265.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(790..2337)
                     /gene="nnr"
                     /locus_tag="JQH22_004156"
     CDS             complement(790..2337)
                     /gene="nnr"
                     /locus_tag="JQH22_004156"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFB2208266.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            2336..3475
                     /gene="queG"
                     /locus_tag="JQH22_004157"
     CDS             2336..3475
                     /gene="queG"
                     /locus_tag="JQH22_004157"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFB2208267.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(3458..3511)
                     /locus_tag="JQH22_004158"
     CDS             complement(3458..3511)
                     /locus_tag="JQH22_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208268.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          901 a         1049 c          944 g          846 t
ORIGIN      
        1 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
       61 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
      121 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
      181 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
      241 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
      301 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
      361 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
      421 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
      481 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
      541 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata gaccattaag
      601 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
      661 cccagagcct gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
      721 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
      781 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
      841 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
      901 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcctgcac aggctgcatc
      961 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
     1021 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
     1081 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
     1141 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
     1201 ggcctcgcta ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
     1261 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
     1321 ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
     1381 ttccaggctt tcggtaagag agtccatagt cagttcatgc accatcaatt ctggtcgtgc
     1441 agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac cagcaccagc
     1501 acgtagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
     1561 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
     1621 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
     1681 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtgagcaa
     1741 gcctagtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
     1801 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
     1861 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
     1921 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
     1981 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
     2041 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
     2101 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
     2161 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
     2221 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
     2281 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
     2341 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
     2401 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
     2461 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
     2521 cgctcgcccc catgagttat tacccggtac gctgcgtgta atcagcgtgc ggatgaatta
     2581 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
     2641 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
     2701 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
     2761 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
     2821 tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg tcgatattcc
     2881 gctgcccgtg gatcaaccag tcgaggaagg atgcggcaaa tgcgtggcct gtatgacgat
     2941 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
     3001 tctcaccatc gaacttgaag gggcgctccc ggaagagttg cgaccgttaa tgggaaaccg
     3061 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
     3121 tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca ttgagttatt
     3181 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
     3241 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcccctt gggatgaaac
     3301 gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttctcgatg agcacatagc
     3361 gtgggcgatt gcgcagcaaa tagagagacg aaatgcgtgc atagtcgaag tgcaattgcc
     3421 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
     3481 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgaataa aatcgtcaag
     3541 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaat
     3601 accattcacc gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact
     3661 ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgta
     3721 gatattttat tgagagaaga
//
LOCUS       DADLDT020000234         3737 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000234 DADLDT020000000
VERSION     DADLDT020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.240"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            368..2020
                     /locus_tag="JQH22_004159"
     CDS             368..2020
                     /locus_tag="JQH22_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFB2208269.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDPNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JQH22_004160"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JQH22_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="JQH22_004161"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="JQH22_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..3457)
                     /locus_tag="JQH22_004162"
                     /pseudo
     CDS             complement(2527..3457)
                     /locus_tag="JQH22_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1031 a          861 c          859 g          986 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccctaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca tcatcggcgt
     2941 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3001 ggagcagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3061 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3121 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     3181 catcaaagaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     3241 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     3301 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     3361 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     3421 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     3481 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     3541 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     3601 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     3661 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     3721 tttactccgt ccctgta
//
LOCUS       DADLDT020000235         3712 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000235 DADLDT020000000
VERSION     DADLDT020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.241"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="JQH22_004163"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="JQH22_004163"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFB2208270.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="JQH22_004164"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="JQH22_004164"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFB2208271.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="JQH22_004165"
     tRNA            2656..2731
                     /locus_tag="JQH22_004165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3712)
                     /locus_tag="JQH22_004166"
     CDS             complement(2746..>3712)
                     /locus_tag="JQH22_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFB2208272.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWES
                     IEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          852 a          933 c          993 g          934 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc attaaaatca ataagttaca catcattagt
     2761 accttcctta ttttttgact gggacaaatt tgggaccgat gggttcagga tcgagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgacaac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgattccc
     3121 gatttgtttt ttcaatacgc gacatgcagt atcattcagc gcaacgccaa ttgcgcggtt
     3181 tgatttactc tcttccgggt ttatccatgc cacccggcgc tgcatatcta tttgttgcca
     3241 ttcaaggttg atgatgttcg agcgtcttaa gcctgttgcc agtgcaaatt caacaacaga
     3301 ctttaatggc tccggacatt catcaatcag cctttgtgct tcatggggct ccagccagcg
     3361 gatccgttta ttctttggtt gaggcacttt aataattggt gccttatcca gcattttcca
     3421 ttcacgctct gcggctctta gtagggcctt tataaatgaa agatgcgtag ccttcgttgc
     3481 aacggacgct ggttttggcg tgtattctgg aacaggtttc cctttttttc tgcatgcttc
     3541 tgccctgagt ttccagtttt cctcatgacg ccggttcgtc attttctgca ttgctgaata
     3601 aatttttgat tcagtgatgt ctcttagttg catccctgcg aaatgttgaa gccagaatcc
     3661 gatccggctt ttgtcatcgt ccagtgattt cttatgtgct ttctcttcga gc
//
LOCUS       DADLDT020000236         3647 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000236 DADLDT020000000
VERSION     DADLDT020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.242"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..182
                     /locus_tag="JQH22_004167"
     CDS             <1..182
                     /locus_tag="JQH22_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFB2208273.1"
                     /translation="APDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLW
                     SDADPVPPWIWRHRK"
     gene            228..437
                     /gene="hha"
                     /locus_tag="JQH22_004168"
     CDS             228..437
                     /gene="hha"
                     /locus_tag="JQH22_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFB2208274.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            476..1066
                     /locus_tag="JQH22_004169"
     CDS             476..1066
                     /locus_tag="JQH22_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFB2208275.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1306..1566
                     /gene="repA"
                     /locus_tag="JQH22_004170"
     CDS             1306..1566
                     /gene="repA"
                     /locus_tag="JQH22_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFB2208276.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1790..1864
                     /gene="tap"
                     /locus_tag="JQH22_004171"
     CDS             1790..1864
                     /gene="tap"
                     /locus_tag="JQH22_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFB2208277.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1857..2714
                     /gene="repA"
                     /locus_tag="JQH22_004172"
     CDS             1857..2714
                     /gene="repA"
                     /locus_tag="JQH22_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFB2208278.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3414..3605
                     /locus_tag="JQH22_004173"
     CDS             3414..3605
                     /locus_tag="JQH22_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208279.1"
                     /translation="MADSGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          990 a          894 c          916 g          847 t
ORIGIN      
        1 atgcaccaga cggaatgaat attaatcagt ttatggtccg ggccggggct gcctgggtat
       61 atgagcagta caacacagat cccgtgctac ctgtccttca gaacgaagcc agacaacaaa
      121 aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggagg caccgtaaat
      181 aaagcgatgg caatcctatc attcatctgt tctcagcggg aataaaaatg gcaaaaacaa
      241 aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg gaaaaaatta
      301 ttcataaaaa cagagacagt ttatcaaaca gtgaacgtga atcatttaat tcagcagcag
      361 accaccggct agcggaatta atcaccggaa agttatatga caggatacca aaagagatat
      421 ggaaatatgt aaggtaataa ttcagcaata attaattgcc aggagcagat aaataatgaa
      481 gttgattatt tttattctca ttgttctgat tattgcagcg ctacttatcc ggattatcct
      541 cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag gtatacggac
      601 tggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga agacgctgct
      661 gacagaaagg gaagtcagtt ttatgaaagg actgttcaga attgtggata tgaagcggtg
      721 gtatctgtgt ccgcaggtac gggtagcgga tatcgtccag ctgaacggga atatccggcc
      781 acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg ttgatgtggt
      841 tatcgttgag ctgcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca
      901 tttacgaccg gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg
      961 tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct
     1021 gaatacaaca ggggctgatc agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc
     1081 tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat
     1141 aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga
     1201 tttgtgcgcc aacacagatc ttcgccacaa ttctcaagtc gctgatttca aaaaactgta
     1261 gtatcctctg cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa
     1321 aatgcagtga cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact
     1381 gactctgaga aacaaatggc cgctgttgca agaaaacgtc ttacacacaa agagataaaa
     1441 gtttttgtca aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata
     1501 acacaggctc agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta
     1561 aagtaaagac tttactttgt ggcgtagcat gctagattac tgatcgttta aggaatttta
     1621 tggctggcca cgccataagg tggcagggaa ctggttctga tgtggatgta caggagccag
     1681 aaaagcaaaa accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggcc
     1741 cacttaaacc gtatagccaa caatcaagct atgcggggag tatagttata tgcccggaaa
     1801 agttcaagac ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga
     1861 ctgatcttca acaaacttat taccgccagg taaagaaccc gaatccggtg ttcacacccc
     1921 gtgaaggtgc ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca
     1981 cctcccgttt tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc
     2041 gcatgccacc ggtactacgt cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc
     2101 actatgaccc actggccaac cgcgtccagt gttccatcac cacgctggcc attgagtgcg
     2161 gactggcgac ggagtctgct gccggaaaac tctccatcac ccgggccacc cgggccctga
     2221 cgttccttgc agagctggga ctgattacct accagacgga atatgacccg cttatcgggt
     2281 gctacattcc gaccgatatc acgttcacat ctgcactgtt tgctgccctc gatgtatcag
     2341 aggaggcagt ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa
     2401 agcaggggct ggataccctg ggtatggatg aactgatggc gaaagcctgg cgttttgttc
     2461 gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg
     2521 cgcgtcgtga tgcggacagg cagcgtcagg atattgtcac cctggtgaaa cggcaactga
     2581 cgcgtgaaat ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg
     2641 agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc cggctggcca
     2701 cagcttcccc ctgaaagtga cctcctcaga ataatccggc acgcgccgga ggcattcgca
     2761 cgcctgaagc ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca acctcatcat
     2821 ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct cacagacggg
     2881 gaattttgct tatccacatt aaactgcaag ggacttcacc ataaggttac aaccgttcac
     2941 gtcataaagc gccagacgtc aaccctacag ggtgcaatgt atcttttaaa cacctgttta
     3001 tatctccttt aaactactta attacattca tttaaaaaaa aaacctgttc actgcctgtc
     3061 ctgtggacag acagatatgc acctcccacc gcaagcggcg ggccccaacc gaagccgatt
     3121 tagttacaac atgcaaacat aaacccctaa aaaacccaag tccaacacag aactgaaacc
     3181 acaacgcccc cctcataact gaaaagcgcc gccgcccccg cccaaagggc gggaacagag
     3241 tcgctattaa ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctggctggac
     3301 gtatcgagta cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     3361 cttcgggtaa aaccctcgtc gggaccactc cgaccgcgca caaaagcatt ttcatcgccg
     3421 atagcgggta tggcttggca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc
     3481 ggctgacgcc gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc
     3541 ttccatgccg ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag
     3601 tgtgagtaat attaagtggt cgtttcaggg atatcgttaa gacttga
//
LOCUS       DADLDT020000237         3590 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000237 DADLDT020000000
VERSION     DADLDT020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.243"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            32..493
                     /locus_tag="JQH22_004174"
     CDS             32..493
                     /locus_tag="JQH22_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFB2208280.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="JQH22_004175"
     CDS             complement(534..1004)
                     /locus_tag="JQH22_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFB2208281.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="JQH22_004176"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="JQH22_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFB2208282.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="JQH22_004177"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="JQH22_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFB2208283.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DADLDT020000238         3547 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000238 DADLDT020000000
VERSION     DADLDT020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.244"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="JQH22_004178"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="JQH22_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFB2208284.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="JQH22_004179"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="JQH22_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFB2208285.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQH22_004180"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQH22_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFB2208286.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctgg
//
LOCUS       DADLDT020000239         3447 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000239 DADLDT020000000
VERSION     DADLDT020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.245"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            30..509
                     /locus_tag="JQH22_004181"
     CDS             30..509
                     /locus_tag="JQH22_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208287.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            497..730
                     /locus_tag="JQH22_004182"
     CDS             497..730
                     /locus_tag="JQH22_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFB2208288.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            730..2196
                     /locus_tag="JQH22_004183"
     CDS             730..2196
                     /locus_tag="JQH22_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFB2208289.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2087..2830
                     /locus_tag="JQH22_004184"
     CDS             2087..2830
                     /locus_tag="JQH22_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFB2208290.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2834..>3447
                     /locus_tag="JQH22_004185"
     CDS             2834..>3447
                     /locus_tag="JQH22_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFB2208291.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDA"
BASE COUNT          878 a          844 c          981 g          744 t
ORIGIN      
        1 tcatcaaact gtcttggata gaagccgcag tggatgcgca caaaacgtta aatttcgaac
       61 ccagtggaag aaagcgtatt ggctttgacg tggctgacag tggtacagat aagtgcgcta
      121 acgtttaccg tcacggatcc gttgttttct gggccgacga atggaaggcc aaagaagatg
      181 aattactgaa gagctgccag cgtacttatc aggcggcgct ggagcgtgaa gcagatattg
      241 tttacgactc tatcggtgtt ggtgcgtctg ccggtgctaa attctctgaa attaacgctg
      301 accggaagag cgagaacgca tacgcgcgac gtgtgaatta ccagaggttt aacgccggtg
      361 ctggtgtgca tgagccagat gacgagtaca acggcatccc caacaaagac tttttcgcaa
      421 atcttaaggc tcaggcatgg tggctgaccg tttcagaaat acgtttaacg ccattaacaa
      481 cggagaacag tatcctgtgg atgagctgat cagcatagat tctcgttgtc cgttgcttga
      541 aaagctgaaa ctggaactga caacacctca tcgtgatttc gaccgtaacg gacgtgtgat
      601 ggtcgaaagt aaaaaagacc tcgcaaaacg cgagataccg tcaccaaacg ttgctgatgc
      661 attcattatg gccttcgcgc caatcgatac atcgctggat atctgggaac agctggggag
      721 acaggcctga tggcacgaaa caaacaagcc ctgcggcgaa ctgtgcaggc cacagctgat
      781 ggttatgaga attttattgc ccgagtaggg atgcagacac ctaaccagca ctcagcatcc
      841 acctaccggg ctaatttcac cagtcgtaac cgcatgctgg tggaatggtc ctatcgttca
      901 tcctggatca tcggcgaagc agtcgatgct atcccggatg atatgacccg caaaggcatt
      961 cgcatcactt cggaaattga tgcaaaagat cgtggcattc tcgaatcaca actggatgag
     1021 ttgcaaatct gggatgcgct gaatgacgtg ctgaaatggt cgcgcctcta cggcggcgcg
     1081 gtgggtttca tcatgattga ggggcaggca ccaatgaccc cgctgcgacc cgaaaccatc
     1141 ggtaagggca agtttaaggg gattctcccg ctcgaccgct ggatggtcga cccggcactg
     1201 acccgccgca ttaaagatat ggggccggac ctgggtaaac ctgagtttta cgatgtggtg
     1261 accacagcaa cgggaattcc tgcctggcgc attcatcaca gtcgcctgat tcgctttgat
     1321 ggcgtcacgt tgccatttca gcagaagatg accgagaacg aatggggaat gtcggttgta
     1381 gagcgtatct gggatcgtct taccgcgttc gacagcgcta ctgtcggcgc ggcgcagctg
     1441 gtctacaagg cgcatttgcg tacctacagc gtggagaagc tacgcgagct tatcgcactt
     1501 ggtggtcctg cgtatgaagc gttgctgaag aatatcgacc tgattcgaca gttccagagt
     1561 aatgaaggca tgacgctcat ggactcgcgg gataagtttg aaacccatca gtacagcttc
     1621 agtggtctgg atgacatcct ttcgcagttt gcagaacaga ttagtggcgc tgttggtatc
     1681 ccactggtgc ggttgttcgg acagtccccg aaaggatttt ctaccggtga tgcagacctt
     1741 gccaactatt acgatcgcat cagttcgttg caggagaggc gtttacgtct tccggtgcgg
     1801 cggatactgg acatcatgca tcgttcggaa cttggcaagc cgctgccgga cgatttcacg
     1861 tttgagttta acccgctctg gcaaatgtct gatgtcgatc gctcaacggt ggcgttaaac
     1921 accaccaacg caatcagtac ggcgctgggt gatggtctga tgacactgaa agccgctatg
     1981 actgatttgc gagaaaattc tgacgtaacc ggcatagggg catccattac caacgaggac
     2041 atcgagaatg ccgaagatga agcgccgccc ggcatcggcg aacctgatga cgaaccgcag
     2101 gagccgtcag gcggaaatcc ggtatcgaac cagcctacgc aggatagcga gggcggtcgg
     2161 agacatcgta aatggtcgct acgatggttc aaatgacagt atcacggaaa ttattgcggc
     2221 gctggagcgt tacagtgaaa tcatcacccc ctgggcgaca aaggtcgcgg aaaactttac
     2281 tgcggaccta acccggcaga acgagaaagt ttggcggcaa cacagcaaga acatcagtcg
     2341 cgagctccgc aatcttgtgg aaagcgctcc tgtgggccag gtgatgcaat ccatcatcgc
     2401 cgaacaggtc aagtacatca aatctctgcc tctcgaggcc gcagacaggg tgtacgacat
     2461 ccagaacaaa gcgatagagg ctgttgtcac cggtggtcgg gcggagcagt ttgctaaaga
     2521 gattgcatct accggcgatg ttgctaagtc tagggccgat ctgattgccc gaacggaact
     2581 gggaagagca acgggcgcgc tggatatggc ccgagcgata gctattggct cggatggtta
     2641 tatttggcgt acagccgatg atggcgatgt ccgagattcc cacgatcaca tgaaaggtaa
     2701 attcgtccgc tgggattcac ctccaacttt ggacggcatg accggccacg cgggcgagct
     2761 gccaaactgc cgctgctata aagagatcgt gtttgttcgc gttccattcg caatgaaaag
     2821 ggcagcataa cccatgaaat acttttttga gaccaggctc ggggaaaccc gataccgcct
     2881 ggctgacggc tcgttgctgt gcaaagacgt gccgatagga cgaacaggta agcagctcta
     2941 tggtgctgat gacctgccaa aactgaaacc cgataagttc gttgaaatag tcgtcacgcg
     3001 ttctcctgag caggtattcc atccggccac gcttgcctca ttcgaaggga tgagcatcac
     3061 gatcctgcat cctgaagatg aaaacgggaa tgtgcggctg gtaaatcccg agaactggaa
     3121 agtgcttgct gtcgggcacc tccagaatgt ccggcgcggg acgggtgagc agtctgattt
     3181 gatgctggct gaccttatcg tcaaagacga aagcgccatt cagcttatcg aagatggcct
     3241 gcgcgaagtg tcgtgcggct atgacgcgga gtacgagcag accgagccat gtaaagccga
     3301 gcaggtcgat attaccggaa accatgtggc tcttgtccct aaaggcagag ccggaaatcg
     3361 ttgtgcaatt ggagacagag acacaatggc aaatcaaaag aaaaactggt ggaaccgcat
     3421 gcgtgcggcc atcaagacag gagatgc
//
LOCUS       DADLDT020000240         3412 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000240 DADLDT020000000
VERSION     DADLDT020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.246"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(226..429)
                     /locus_tag="JQH22_004186"
     CDS             complement(226..429)
                     /locus_tag="JQH22_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208292.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="JQH22_004187"
     CDS             complement(431..586)
                     /locus_tag="JQH22_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFB2208293.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="JQH22_004188"
     CDS             complement(789..1196)
                     /locus_tag="JQH22_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208294.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="JQH22_004189"
     CDS             1273..1500
                     /locus_tag="JQH22_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFB2208295.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="JQH22_004190"
     CDS             1484..1906
                     /locus_tag="JQH22_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208296.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2768
                     /locus_tag="JQH22_004191"
                     /pseudo
     CDS             1984..2768
                     /locus_tag="JQH22_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2775..3412
                     /locus_tag="JQH22_004192"
                     /pseudo
     CDS             2775..3412
                     /locus_tag="JQH22_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          884 a          750 c          917 g          861 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatataaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     2341 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagtgga
     2401 agaggaagag gaagaggaag atctaaatac tgatctcccc ctaaatcccc ctcgccaaaa
     2461 acgagcgtct aaaaaattcg agccggaggc tattgagctg cccgattggt tgccggaaac
     2521 actctggcat gagtgggtcc ggttcagaca ggcattgcga aaaccgattc gaacggagca
     2581 gggcgatacg ggaactggaa aaattccgtc agcagggttt tacacctgag caggtgattc
     2641 gacacagcat cgccaatgaa taccagggcc tgttcgcgcc gaaaggtgtt cggcctgaga
     2701 cgttgctccg acaggttaac accgtctcgt tgccggacag tgcgatcccg ccaggcttca
     2761 gggggtaacg gaccatgaaa aatattgcga caggcggcgt tctggaacgc atccgccgac
     2821 tgaccccgca acatgtaacc gccccattca gaacggtagc ggagtggcgc gagtggcaac
     2881 ttgctgaagg ccagaaacgt agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg
     2941 aaaaaattct gaatcgctca ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt
     3001 accaggtgca gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg
     3061 aactgatgac cgggtgtaca aattttgcgt tcagcggaaa acctgatacc gggtagaatc
     3121 acttagcggc agctatcggg aatcgcctgc tgaaagacgg tcagacagtg attgtggtta
     3181 ccgtggctga tgttatgagc gccctgcacg ccagctatga cgatgggcag tcaggcgaaa
     3241 aatttttgcg ggagctgtgc gaagtggatc tgctggttct tgatgaaatt ggcattcagc
     3301 gcgagacgaa aaacgagcag gtggtgctgc accagattgt tgatcgccgg acagcgtcga
     3361 tgcgcagcgt ggggatgctg acaaacctga actatgaggt gatgaaaaca tt
//
LOCUS       DADLDT020000241         3335 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000241 DADLDT020000000
VERSION     DADLDT020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.247"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..890)
                     /locus_tag="JQH22_004193"
     CDS             complement(<1..890)
                     /locus_tag="JQH22_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2208297.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="JQH22_004194"
     CDS             1131..1931
                     /locus_tag="JQH22_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFB2208298.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="JQH22_004195"
     CDS             2009..2779
                     /locus_tag="JQH22_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFB2208299.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..>3335)
                     /locus_tag="JQH22_004196"
     CDS             complement(2827..>3335)
                     /locus_tag="JQH22_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFB2208300.1"
                     /translation="LKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQ
                     STLVANNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGA
                     GSSAQRLANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFV
                     KNNNMPDS"
BASE COUNT          810 a          824 c          834 g          867 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagct
//
LOCUS       DADLDT020000242         3285 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000242 DADLDT020000000
VERSION     DADLDT020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.248"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQH22_004197"
     CDS             complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQH22_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFB2208301.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2372..2524)
                     /locus_tag="JQH22_004198"
                     /pseudo
     CDS             complement(2372..2524)
                     /locus_tag="JQH22_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2580..3273
                     /gene="ospZ"
                     /locus_tag="JQH22_004199"
                     /pseudo
     CDS             2580..3273
                     /gene="ospZ"
                     /locus_tag="JQH22_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT         1036 a          607 c          576 g         1066 t
ORIGIN      
        1 gcaattttta gccatgaaaa cgtaatcaac tcggtgaacc aattcaaaaa atatacttta
       61 tacctgcgga agtgagaaaa ccaatatatt caatacttgc actattttta atggtgccag
      121 atatattggt actttgctca taaagagatg tatcaccttt tttattagaa atgtaatttc
      181 tggagccttc tatataaaat tttgcactgg gggtgacata gtatccaata ttcgcagcaa
      241 cagagcagta attttggttt tttatttcat ctttaaaaat tgtctcagtc tgatagtgtt
      301 tatctgtatc agatgaaaga acccaaccac tatatttcag ttctgctcca aactcaaaat
      361 tctcatggcg gtaattagca gttaatccaa tataaggtat tttgaagagc tgtttgtaac
      421 ctattgttct ttcaccacta ggatgtgccc cttttttatt cctgctcccc ccgttttcac
      481 tataaatata actccctccc attgcattaa aactgtaacg actctcttgg taacctgcta
      541 ttagtccaag tcgataatcc aaattattta ataaccaacc tttaagattc agctcgaatt
      601 cgttagcatc acggagatgc gtattgggat gccagctctg gtcggtccat acctgagggg
      661 tattggaatt gttccagtct ttatcaacca tgcttgcttt ctggttacct aaagtagtcc
      721 aaccggaaac tccgaaagac acttttggaa ttagcttcca atcgatgcca cctctaacaa
      781 tagtcgcatt actgtatttc cagtccagtt gactaatttt tctcccccct tccttgggat
      841 gataaacgcg ttcttttgtt ttgccactca gagatcctag actaatatcc gtactgatgt
      901 tgtccggtat gaaaagtgga tagttagtgg tagcatgtgt agtaaagatc gcaggtacac
      961 aaagtgcaag tacaaagaat tttaatttca tcgagaactc tacttttttg gttgaaatgt
     1021 ccatgatctt cttactttat aagataaacg tcaatgccaa gcattatttt aattaaatat
     1081 ttgtttatgt taccaagaac atatctttta ttataatatt tattgaactt ttgtaaaaat
     1141 aaaaaaatac ctcattgtgc gaataaagta acgggggcca tagatttctc tacctacctt
     1201 ttggtgttca tacctgaggc tgatagtttc aggattccgc gacaattcta ctcaccttct
     1261 tgttctctcg ccaatatgaa atcaatcaga aaagcgttcc ataagaacaa cgataagaca
     1321 ataaattgtc atcacggctg caatagccaa cgtgcctttc agtaccaaca tgacaacctc
     1381 ctgtatttga cgtaacgtgt gctgataaat atgtatttgt atctcaactg actcaataca
     1441 acacttaagt atagtttttc agattttgtt tttcccgtga aatattatgg aaatggcaga
     1501 agcaatacat tgaattccga ttcgagtgaa tacggctagc gggcacctca ctttagcact
     1561 gaagccatca acttttattt gacttatttc aatggaaagc tttctcacta tcagtccagg
     1621 taattcccat ccttttaaag tcgagagtca gactgcgact aatacagtta aagtgaccaa
     1681 actcagttat cctctacaca ttttatctca tgtttacttg gacaattcat tggtgctttt
     1741 ataaaagaat aaatattcta ctaatttaat gcatcaccta aaatagtatt ttttataata
     1801 gaactgtatc tatagtatat tattaaagct taattattta ttatgcttta atgtggttgt
     1861 ccgattaagg actaatcctt gaaaacgccg tatagggcga gagttcactc tattgcgata
     1921 acgtaaccgt acaatattgt gcgtttccca aattgaccac tactgaacta ttctgggatt
     1981 taaatgcaat aaaatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     2041 ttccagtgcc agatcttact tataataaag aggcttggca gtttggataa gctattgtcc
     2101 ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt tcatactgaa
     2161 atccccggaa gtattcaatc aaacaattac accataaaag gaagagggag ccccaattaa
     2221 gttatcaagc atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca
     2281 tataaaacgt ctttattgat cctacccaca taatatggac gcagtcgtaa gtgaggctct
     2341 gtgagcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2401 ccggactgag gccgccacac caacggtgcc gtcgccagcg attgtaattg cactcgatat
     2461 aattaaacac tgttgcccgc actatttccc gatagataaa gtgttctccg tggagacatt
     2521 ccacttagga acaattatat tgttcatggt aatgtttata tttgagtata gagattaaaa
     2581 tgattagtcc catcaagaat attaagaacg tatttccaat aaatactgca aatactgaat
     2641 atatagtgag aaatatttat cctagagttg aacatggtta ctttaatgaa tcaccaaaca
     2701 tatatgacaa gaagtatatc tctggtataa ccagaagtat ggcacaatta aaaatagagg
     2761 agtttattaa tgagaaatcc agacgactta attatatgaa gacaatgtat tctccatgtc
     2821 cggaagattt tcagcctatt tccagagccg aagccagtac accggaagga agctggttga
     2881 cagttatatc tggaaaacgt cctatggggc aattttctgt ggatagtttg taccatcctg
     2941 acttacatgc attatgtgag ctcccagaga ttagttgtaa gatcttctct aaagaaaata
     3001 gtgatttttt gtatataatt gttgtattca gaaatgattc tccccaagga gaactacggg
     3061 caaatcggtt tatagaacta tatgatataa aaagagagat tatgcaagtg ttaagagatg
     3121 agtcgccaga gattaaagtc tattaaatct gagattatta ttgctcgtga aatgggagaa
     3181 ctttttagtt atgcatcaga ggaaatagat agctacataa aacaaatgaa tgacaggctt
     3241 tctcaaatca aggctcgaat gcctgttact taatgtatat taatt
//
LOCUS       DADLDT020000243         3258 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000243 DADLDT020000000
VERSION     DADLDT020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.249"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..441
                     /locus_tag="JQH22_004200"
                     /pseudo
     CDS             1..441
                     /locus_tag="JQH22_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            1025..1741
                     /locus_tag="JQH22_004201"
     CDS             1025..1741
                     /locus_tag="JQH22_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208302.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2293..2697
                     /locus_tag="JQH22_004202"
     CDS             2293..2697
                     /locus_tag="JQH22_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208303.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2760..3258
                     /locus_tag="JQH22_004203"
                     /pseudo
     CDS             2760..3258
                     /locus_tag="JQH22_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          910 a          673 c          750 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagacccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccg gtttaatctg
      301 gtgataaagg cgttttacat gcgcctactt gcagccggaa acgccaaaaa agtggcactg
      361 gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa
      421 tggaatgaat cgtatttatg aaaactggat tgtgggccat aaatattcca aaaagatagc
      481 ccgctccgtt tattaccgga acgggcgaga gcatgacatt gtctgttatt ataatgagac
      541 tgctgattct acttattttt aatttcaaga aaagcgtttt ctacaaagaa ttatgagatt
      601 taatgttttg tttagacaat attatcattc tgttcaataa tgaaagtctt catcaacaac
      661 aagtaaaaac aacgtccctc tcatcgaacc ggacttgcag atttcccgca tccggctctc
      721 ttgcaacaca tcagaccttc acgttcgggc ggttatgggg aaggtccagc aagctgtatg
      781 agccctaact gtttgaatac cagtagatta ccagaaactt ctatcgattg aagacattac
      841 caactagcgt tactaaatgt tgaccaagtt ttgttcactt gtctggtttt gatagtagca
      901 ttctgcagag tattttgata tgataaatat gcattaacaa tgatattatt gttaatttcc
      961 tagcatgaaa attaaaagat caacttttat ctcgaatatt ttttacatta tcagttggtt
     1021 tcttatgaat gacaattcat tgcttagaaa ttcttcatta ttcatcgcct atatgggatg
     1081 tgtagggtgg gtcagtgctt attcttatgg ctggggaacc tctttttact atggatttcc
     1141 atggtgggtt gttggtgcag gtttggatga tgttgccaga agtttactct atgctatcat
     1201 agtaatgggt atacttttta ccggttgggg gattggtatt ttattttttc ttcttataaa
     1261 gaaaagaagt aaaatacaag acttgagttt ttttagacta ttttttgcta ttaccctact
     1321 gtttttccct gtaatattcg aacttttaat tctcaaacaa tatttcatac ttccattatc
     1381 actttcattc attattagtt cattggttat ttcaataata attcgtatat atggacggat
     1441 cttttctgta tcatgttttt cagatatccc ttttgttaga gaacaccgca ttaagttaat
     1501 tatggctggt tttttggttt atttttggtt tttttccttt cttgttggct ggtataaacc
     1561 tcaattaaaa aaagaatatc agatgctttg ttataataat agttggtatt atgttcttgc
     1621 tcgttatgat tcacggctcg ttttatctag ttcatttaaa gatgacagta acagattctt
     1681 aatatttaat acagaacagt cgggatttta tgaaatcaat gatgtttatg tcagaaagta
     1741 ataatcctct cccacaaagt gaattaaatg aatatataat gcgttcgaag tatatgtatt
     1801 gagctgtcat actgagaaac aaaccttagg gcagcacaat taaagccaga aacaactatg
     1861 caagaaagtt actaaataag tgctcggaac agtaaaacac tcagggggga ctcagtccgg
     1921 attatgtaat cagataaatt gccaaacatc acaaatcacc agccggactg agtgatgccg
     1981 agcattgcac caataccccg tgatgaacga tgcctgatgc agaaagctcc ctataaaaca
     2041 aagaacaaaa actacgaccg gcgattcacc gtcatactga tgctgcatcg tggtgacact
     2101 gtctgtcact ttcatgttga ctgaacaact ctgctgcgaa gaaggtgtac cgccggcggg
     2161 atttgattgt ccggcgtcta gcttccactt ccctgatgga actgaaagtt gttatggccg
     2221 gtcagcttgc cctgtccgca ggacatgagg tgctgtgacg gacagcacca gtaacggaaa
     2281 gtgacaatga aaatgaatac tgcgctgata gttgcactaa tgtgtatgtg gtatgcggta
     2341 cccgctgctg caaaagagac tttactggct atgccacgta attcaacaga acattgctat
     2401 gcggagatta atgtgcacgg accatatggg gtgtatttcc gagtggtccc gcatcccccc
     2461 gggggaaaga gctgggtcga atgtaacagt gactactatt actctgataa accaccggga
     2521 gtacagatac tcggaacccg tgcagggtgt cgggtatacg gaatctgcgg gaccacctcg
     2581 acactgcatg tggctggaag aggggttgtg tgtattaaaa atatatgcag tccacgtggg
     2641 atgataattc acaggatacg gaaacgtcct gtagtggctg taagcgatga gatgtgatct
     2701 tacccagcaa tagtagacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
     2761 tgacaaaaac agtatcaacc agcaaaaaaa cacgtaaaca gcattcgcct gaattttgca
     2821 gtgaagccct gaagcttgct gaacgcatcg gtgttgctgc cgcagcccgt gaactcagcc
     2881 tgtatgaatc tcagctctat gcgtggcgca gcaaactgca gcaacagatg acctcgtctg
     2941 agcgtgaaag tgaactgccc gcctgaagcg gcaactggcg gagcaggcag aggaactggc
     3001 tatcctccaa aaggccgcga catactttgc gcagcggctg gtatacgtgg tgtcagcgtc
     3061 ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt gtcctcgcgg
     3121 cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat gaactgcgtg
     3181 ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt caggggctga
     3241 gggcaaaggc ctcccgga
//
LOCUS       DADLDT020000244         3221 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000244 DADLDT020000000
VERSION     DADLDT020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.250"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..140)
                     /locus_tag="JQH22_004204"
     CDS             complement(<1..140)
                     /locus_tag="JQH22_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFB2208304.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IA"
     gene            complement(144..278)
                     /gene="pyrL"
                     /locus_tag="JQH22_004205"
     CDS             complement(144..278)
                     /gene="pyrL"
                     /locus_tag="JQH22_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFB2208305.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(559..954)
                     /locus_tag="JQH22_004206"
     CDS             complement(559..954)
                     /locus_tag="JQH22_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFB2208306.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(1086..1799)
                     /gene="bdcA"
                     /locus_tag="JQH22_004207"
     CDS             complement(1086..1799)
                     /gene="bdcA"
                     /locus_tag="JQH22_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFB2208307.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1870..2463
                     /locus_tag="JQH22_004208"
     CDS             1870..2463
                     /locus_tag="JQH22_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFB2208308.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIAKRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2608..3060
                     /gene="tabA"
                     /locus_tag="JQH22_004209"
     CDS             2608..3060
                     /gene="tabA"
                     /locus_tag="JQH22_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFB2208309.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT          854 a          743 c          780 g          844 t
ORIGIN      
        1 ctggcaatga ctttgtgctt caacagctct ggttgcgggt ttgctttcag tttcgccgct
       61 gtcgccagca ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat gatatgtttc
      121 tgatatagcg gattagccat cttttatctc ctgacaccta ggcaaaaaaa agcccctcga
      181 ttgaggggct gggaatgggt gatcaacggg aagaaaaacg gcaggccagc gtcttttttc
      241 agacgcggta agacaaaatg tcgaacacac tgaaccatac atcctcccgg caaattgtcc
      301 ggcattatac tcatagccag aagcggtgca agcatttgat gcaatatttt gtcagcgcaa
      361 acggtttatt tgaattaaaa gtcaaaatat atgcattttt attttctatg attctggagg
      421 ggaagtgtcg ttcggtatca ataattgcag acatttcctg cttttttctt tttcattttc
      481 acgcaatcag aaatgcattt tattccattc atccgactta tagggcggag ggtgaaaacg
      541 aacggctaac actattgctt atagctcagg gatacgcgct atcactttaa tttcaaaatc
      601 aaagcctgcc agccaggtaa cacccaccgc cgtccagttt ggataaggtg gggcgctaaa
      661 tatttcattt ttcaccgtca tgatgtcttc aaattgtttt tccggatcgg tatgaaagct
      721 cgtaacatca atgatatcgt caaaagtgca tcccgcagct gccagggtcg catgcaaatt
      781 atcaaaggcc agtctgactt gttgctgaaa atcgggttct ggtgttccgt cctctcgact
      841 tcctacttgc ccggaaacaa acagcaaatc gccggaacga atagccgcag aataacgatg
      901 ctcagcatac agtgaatgtc ggccagcagg gaaaacagcg gttctttcta ccatttggtt
      961 atcctcaaga tttacgacat gaacagaaga tttatattta tcgggagccg cttttagggg
     1021 gacgacgtta gtaaacaaaa cccagacatc atggataatg gctgggctta attgagcgta
     1081 gtcggttatg cgccaaacgc gccatcaatg gtatgcatcg cgccggtaac aaaactggct
     1141 tctggccctg ctaaccatgc gaccatacca gcgacctctt ccggttgccc atgtcttttg
     1201 atggccataa aactatgcaa catatcgcgc attggcccgt tggcgggatt aacgtcggta
     1261 tcaattggtc ctggctggac gacgttgatg gtgatccctc gcggtccaaa atcacgggcc
     1321 agcccgcgcg ccatgccttg cagggcagat ttactggcgg cataagcagc catgcctgca
     1381 acaggcatac gatcgccatt cacggagccg atgattaaga tgcgcccgcc ttcgggcatc
     1441 tgccgggcgg cttcaacaga ggcatgataa ggagcatgaa tattgatttt gaaaaggcga
     1501 tcaatatcgt cggcatttaa ttccagggca tcgccaaaga cgccaatacc tgcatttacc
     1561 accaggatat ccaatgcgcc gctcttacga acgacatcaa taacagcgtc tctgtcagca
     1621 ctatctgtga atactgctgt cgctccagtc tcttgtgcca gacgttcagc ggcatctttc
     1681 gaccccgcat aggtgaatcg tacattggcc ccatcggtga cgaaacgacg tacgatagcg
     1741 gcaccgattc cacgactgcc accgaggatg agaactgtct taccggtaaa agcgcccata
     1801 aggactcctt gatttattat gtaacatgca ttacaaaact gttttaactt tctgtcaaca
     1861 ggttttgtaa tggtcactaa aaaacaatct cgcgttccag gtcgtcccag acgtttcgct
     1921 cctgagcagg cagtctctgc ggcaaaagtg ctttttcacc aaaaaggttt cgatgcagta
     1981 agtgtctctg aagttactga ttatcttggt attaaccccc cgagtctcta cgcggctttt
     2041 ggcagtaagg ctgggttatt tagccgtgta ctcaatgaat atgtcggtac ggaagctatt
     2101 ccgcttgccg atattcttcg tgatgatcgt ccggtaggcg agtgcctggt tgaggtatta
     2161 aaagaagcgg cgcgcagata tagccaaaac ggcggctgcg ctggctgtat ggttcttgaa
     2221 ggtattcata gtcatgatcc acaagcgcgt gatattgcca ttcaatatta tcacgccgca
     2281 gaaacgacca tttatgacta tatcgccaag cggcatccac aaagcgcaca atgtgtgact
     2341 gattttatga gtaccgtgat gtcagggctt tctgcgaagg cacgggaggg gcactccatc
     2401 gaacaactct gtgcaacagc tgccctggca ggggaagcga taaaaactct tctcaaggag
     2461 tgatgctggc cttgatccga aaggcgggaa cgcgcctgcc gataagttgt gataagacaa
     2521 taattcacgc gttaaggctg gcggaattga tcatcttttc gtataacgat agaaatgaaa
     2581 cattatttta attaaggagt ggaaaagatg atcatcggaa atattcataa tcttcagccg
     2641 tggctacccc aggagttacg ccaggcgatt gagcatatca aagcacacgt tacggcagaa
     2701 acgccaaagg gcaagcatga tatcgaaggc aatcgtctgt tttatcttat ctcggaagat
     2761 atgaccgagc cgtacgaagc tcgccgtgcg gagtaccatg cccgctatct tgacattcag
     2821 attgtgttaa aaggtcagga aggcatgacc ttcagcacgc aacctgcagg cgcgccggat
     2881 accgactggt tagctgataa agacatcgca tttttgccgg aaggcgttga tgagaaaaca
     2941 gttatcctaa atgaaggtga ttttgttgtg ttttatccgg gggaagtgca taaaccgctg
     3001 tgcgcagtgg gcgcgccagc ccaggttcgc aaagcagtag tgaagatgct gatggcgtga
     3061 tgacttttcg ccgtaaataa ctccaggttt acggcgagtt tgtgaaaaga gcgttttttg
     3121 atattttttt gtgagtaaaa tttgtaatgc ttagacgttc ttattaactc aaggagttcg
     3181 tcatgggcaa aatatcagat ttgaattatt ctcaacacat t
//
LOCUS       DADLDT020000245         3196 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000245 DADLDT020000000
VERSION     DADLDT020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.251"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..2910)
                     /locus_tag="JQH22_004210"
     CDS             complement(<1..2910)
                     /locus_tag="JQH22_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFB2208310.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHIN"
     gene            3074..>3196
                     /locus_tag="JQH22_004211"
     CDS             3074..>3196
                     /locus_tag="JQH22_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208311.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGS"
BASE COUNT          777 a          834 c          785 g          800 t
ORIGIN      
        1 attgatatgt ccgtggcaaa gcggggagag gcgtgcgata tcttcatcat tcagtgtttc
       61 accctgcgcc cggagatgat ccagggctgc ctgcatataa atagtgttcc ataacacgac
      121 ggcgttagtg accagcccca gtgtgcccag ttgatcttcc tgaccgtcgg tatatcgttt
      181 tcttatctca cctttttgac cgtgacagat ggctctggca acggcatggc gactttctcc
      241 ccgattaagc tgggtcagaa tgcgccggcg gtaatcttca tcatcaatat aattaagcag
      301 atacagcgtt ttgttgatgc gccccacttc aatgattgcc tgagtcagtc cggaaggacg
      361 ttcacttttc agcaatgaac ggaccagcac tgaaacctgt actttgccca gcttcaggga
      421 gccagcggtc cggatcattt cgtcccactg aaggactatt tttcggggat ctgattgccc
      481 tctggcaata tcattcagca cgccatagtc ggcatcatgg tccattcgcc agaaaaccga
      541 agcaccggca tcagccaggc gtggagaaaa ctggtatccc agcagccaga aaaggccaaa
      601 gacaagttcg ctggcacctg ctgtatcggt cataatttcg gttggattca gcccggtctc
      661 ctgttccaga agaccttcca gcacaaagat agagtccctc agcgtccccg gtataacgat
      721 gccatgaaag ccggaatact gatcggacac aaagttgtac caggtgatcc ctctgttatt
      781 accaaagtat ttgcggttcg gtccggcatt gattgttctg actggcgtaa caaagcgcat
      841 tccatctgca gatgccactt ctcctccacc ccatatctgt gccagtggca gcgttgcctg
      901 aaaatcaacc agtctggcat tagcgctggt gatagtttca gcccgcagat agttcgcttt
      961 tgtccagttc agccggtgtc gggtcagtgc aggaacattt gatctgatca gtggttccag
     1021 accgatattg caggcttcag ccatcagcac ggcgctgatg ctgacgggca gatcatcaac
     1081 tctggcactg gcttcactag catggaaaaa ctcatcagca aatccggtat gggcgttaat
     1141 ttcgagcagc aactccgtta aatccaccgg agggagtaga tcactgatca ttttgctcag
     1201 tcgtttcaga ctgtccggct catcaagact ggcgagggga gaaattgtca accggggctt
     1261 cgggccagaa acatcgagtt cgacagcctc attttcgcaa agacgtgcag caacctgtct
     1321 gtaacgacta tcaagctgat gacccagaga ttttattgct tcctgcgggt ctgtcgggtg
     1381 ccccaaagaa cgataaacct taatccggtt tgcctgccag tcagcaccct gtagtaatct
     1441 tgcacgagga tctccccacc ggttactgcc ggtaacgtag acatccctcc gcctcagact
     1501 atcctgcagt ttactgagaa agcagagcgt gtatcccctg cgggtgatat gtttttcctt
     1561 gttaatcacc agccgtttcc atgaccgact gataatttcc gttggtgcgt cgtcaaaaaa
     1621 ctgccgccgt gagctgaact cccggctgag gtagtcacag gcattcagag tggtaacccc
     1681 ggcaggtgcg gatgaaaatt taacggtatt cagcagatgg ggcaggaaac gacgaacgcg
     1741 cccgtactgc tccaccattt cttcatgaaa attatcgtct gagggccggg caatttcacg
     1801 gacaagcgtg atgatttcag ccagcttttg ccttgggatg tagctgaaca cctcagcacg
     1861 aatcgattcg tccggtgttt cttctttcag caggtacgaa catgcgctgg cgagcgccaa
     1921 tgcagattta tccagatcct tcagcgagcg gagccgtttt ttctgcccaa tctttctggc
     1981 gtcacggatg ataacggcca gcatggcgtc cagaacgtcc aatgcatcat ccagcgccag
     2041 cgtttcccat gcaaggacaa aggcaaccag aaccgccatc cttttctgcg gtgacatcct
     2101 ggcaatattg aacaccgaag tcataccagc ataacgtgcg agatttttca ggcgcacagc
     2161 cgggagtgta ctcaggtttt cagcatgcag gccaaaatcg ttcagagttt tccagcgttc
     2221 aattgcttca ttaaacgccg gaccactgat ggtcacaggg ccctttttca gtgattccag
     2281 taaagacagg cggctgcaat cagttggccc cagcagcatc tccagctgtg aacgctgttc
     2341 ggctgacggt atcagtgcca gtttgttcca caggcgcaac gtcgcctttt cccttacctc
     2401 tgaaatcaac cgggtcagcg tagtggctcc ggggagaata atacgatgtt gcataagcca
     2461 ccctgtcgcc agatcgaaaa gcaggccagg acgttcgttg cttatccagc tccgggtata
     2521 taaaagacgg gtaaggcgaa atgtccaggg ccaggcaaat tcacgatact gatagtgctg
     2581 acgtatcagc gctgcatgct cacggcgggt attttccctc tgaccgtatt ctgcaagaac
     2641 ggtgatatca cgaatcccga gctgtctggc ggtaaaatgc cggacgccgg aaggaatatg
     2701 attcatatcg gtgaggaagg tgcccagaaa acggacacat ccaatttgca gggcaatgcc
     2761 cagacggttg tgatcacctc tgctttttcc gataaattcc ttgtctgctt catcaaggtg
     2821 aaaatatcgt gccagctgaa gctcatccgg ttcaccggtg aatctgccat agctttcagt
     2881 ctgctcagtg gtcagaaagt caacgggcat atcggcctcc ctgcctgacg ggcatttagt
     2941 aacatttttc caaccgtacg aaatgttata aattatcgga catcgtaaaa ctgttacatt
     3001 aatatgtcta ttaaatcgta aatttgtaat aatagacatg agttgtccga tattcgattt
     3061 aaggtacatt tttatgcgac tttttggtta cgctcgggtc tcaaccagtc agcagtctct
     3121 tgatcttcag gtcagagcac tcaaagacgc aggtgtgaaa gcaaaccgta tatttaccga
     3181 taaggcatcc ggcagt
//
LOCUS       DADLDT020000246         3178 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000246 DADLDT020000000
VERSION     DADLDT020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.252"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="JQH22_004212"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="JQH22_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HFB2208312.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="JQH22_004213"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="JQH22_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtagtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg gggggaccct gagcaggtgg taattgtaag acgttgcggg
     2821 cgatggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat ggcacagcat
     2881 atcaactgat aaataattcc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
     2941 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
     3001 tgcctgaaat gcccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata
     3061 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
     3121 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcat
//
LOCUS       DADLDT020000247         3112 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000247 DADLDT020000000
VERSION     DADLDT020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.253"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="JQH22_004214"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="JQH22_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFB2208313.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="JQH22_004215"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="JQH22_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="JQH22_004216"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="JQH22_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFB2208314.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="JQH22_004217"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="JQH22_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFB2208315.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="JQH22_004218"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="JQH22_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          772 a          754 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcaccgctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DADLDT020000248         3097 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000248 DADLDT020000000
VERSION     DADLDT020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.254"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(104..1813)
                     /locus_tag="JQH22_004219"
     CDS             complement(104..1813)
                     /locus_tag="JQH22_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFB2208316.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="JQH22_004220"
     CDS             complement(2245..2964)
                     /locus_tag="JQH22_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFB2208317.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          952 a          587 c          519 g         1039 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatt ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgatcatggt gggaaggctt agaacgc
//
LOCUS       DADLDT020000249         3064 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000249 DADLDT020000000
VERSION     DADLDT020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.255"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="JQH22_004221"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="JQH22_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFB2208318.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLLMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="JQH22_004222"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="JQH22_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFB2208319.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3064)
                     /locus_tag="JQH22_004223"
     CDS             complement(2711..>3064)
                     /locus_tag="JQH22_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFB2208320.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          720 a          677 c          753 g          914 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatt
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gata
//
LOCUS       DADLDT020000250         2952 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000250 DADLDT020000000
VERSION     DADLDT020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.256"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..138
                     /locus_tag="JQH22_004224"
     CDS             <1..138
                     /locus_tag="JQH22_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix-type transcriptional regulator"
                     /protein_id="HFB2208321.1"
                     /translation="PPATLAPEIFPDRHWLLVTRGKLTAARKKQIEQWQQQVSLEVII
                     L"
     gene            238..648
                     /gene="uraH"
                     /locus_tag="JQH22_004225"
     CDS             238..648
                     /gene="uraH"
                     /locus_tag="JQH22_004225"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFB2208322.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            751..1710
                     /locus_tag="JQH22_004226"
     CDS             751..1710
                     /locus_tag="JQH22_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HFB2208323.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            1856..2944
                     /locus_tag="JQH22_004227"
     CDS             1856..2944
                     /locus_tag="JQH22_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFB2208324.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT          702 a          856 c          792 g          602 t
ORIGIN      
        1 ccgcccgcca cgctggcccc ggagattttc ccggaccgcc actggcttct ggtcacgcgc
       61 ggtaaactca ccgccgcgcg caaaaagcag atcgagcagt ggcagcagca ggtatcgctt
      121 gaagtgatta tcctctaatc attttctcct gcgggaatct tcacgaaaag ttgaatattt
      181 tcccgcgcca caaatgctaa cgttttcctg tgaccgacaa ccccacagga aaacacaatg
      241 aaaaaaactg cctctctgct gatgctgacc accctggcct ttgcccctgc cgcctttagc
      301 gcccctgccg gcacgctgag cgtacacatt cttgaccagc aaaccgggat gccgccatcg
      361 gacgttaccg tcacgctgga aaaacagcag caggacaaat ggaccccaat tgccagcggc
      421 aaaactgacc atgacggccg catcaaatcg ctttatccgg aggatcagga tatgcagccc
      481 ggcgtctata aagtggcctt taaaaccgca gattacttcc acgggaagaa gctggactcc
      541 ttcttcccgg agatcccggt gctgttcacc gtaacccgga ccaacgaaaa gctgcacatc
      601 cctctcctgc tgagccaata cggttattca acttacaagg gcagttaaga aacgttgatt
      661 ttaaaggcaa ataaaaaata attccgctaa tacttggaca aattagttat gttgtgtaag
      721 ttttaaagag aagaacggga ggaccacacc atgacaacaa aacctgtgct cggtattagc
      781 ggatgtttga ccgggtctgc cgttcgcttc gacggcggac acaagcgtat gggctttgtc
      841 atggatgaac tggcccagtg ggtgagtttc aggcccgtct gcccggaaat ggcgattggc
      901 ctgccgacgc cacgcccggc catacgcctg acgctgacgg acagcggcga aacgcagctc
      961 cgcttcagca agccgcccca tgatgatata acgcagaaaa tggccgactt tacggcggac
     1021 tatctgccaa aaatcggcga tctgtcggga tttatcgtct gcgcgaaatc gccaagctgc
     1081 ggcatggaac gcgtgcggct atatgatgaa aacggcaacc ggggccgcaa ggagggtgtc
     1141 gggctgttca ccgccgccct gcttgaaacc tacccgtggc tgcccgtgga ggaagacggt
     1201 cggctgcacg acccggtgct gcgggaaaac tttatcgagc gggtatttgc cctgcacgag
     1261 ctgaacacgc tgcgcgctaa aggcctgacg cgccgcgcgc tgctggattt ccacagccgc
     1321 tataaattgc agcttctggc gcaccatcag gcgggctacc gcgagattgg cccgttcgtt
     1381 gcgtcgctgc atgaatggga agatctggac gcgttcttcg tggcgtaccg cgaaaagctg
     1441 atgaccatcc tgaaaaagcc cgcctcgcgg aaaaaccata ccaacgtgct aatgcatatc
     1501 cagggctatt tccgtaacca gctgaacagc cgccagcgcg tcgagctgcg cgatgtgatc
     1561 ctgcactatc gcgacgggct gctacccatc ctcgcgccgt tgacgctact gaaacactac
     1621 atggccgaat accctgaccg gtatctgatg acgcaaaact actttgatcc ctatcctgat
     1681 gatttgggtc tgcgtctggc agtaacctga cgataaaaat gcgaaggccc gtttgaccca
     1741 cagaggccca aagcagtacc ccgtttagac gacaccctca ctgtcgtctc ttttttgcat
     1801 tccacacaaa agactgtaat tatgtacagg attgtaacgc aaaaagggac tgcctgtgat
     1861 caccaccctg cacattcaaa actaccgctc gattcgcgaa atgtcgctgg agctggaaca
     1921 gctcaacatc gtctttggcc caaacggcac cggaaaatcc aatatctaca aggcgatcca
     1981 cctgatgcac agcgccgcac aggggcagtt ttcgcaggca ctggcgaacg agggtgggat
     2041 tttaaaagtg ttctgggcag gcaaaacccg cagcgaccag ctgcggcgga tgaatttggc
     2101 cgttgaaacc gaaacctacg aatacgagct gcaggtaggc tttgtcgaaa aactgcctta
     2161 cccctcacag tttcaactcg atccggtgat caaagaagag tccatctggc tgagtggtca
     2221 ataccgccgc ccgtcgtcac agctgatgaa gcgtaaaaat caggccgtat ttctgaacaa
     2281 cgtgcatcac gagaaagtca cccacagcgg cacgctgtat gagaacgaat cggtgttcgg
     2341 ccagcttggc gagccgcatc tttacccgga agtgtcgcag atgcgcgagt cgctgcgtaa
     2401 ctggcgcttt tatcaggagt tttctgtatc aatcggctcg gccatgcgcg cgcctcaggt
     2461 cggtttccgc tccccagtgc tggccagcga cggcgcaaac ctggcggcgg cgtttcagac
     2521 catcgtggaa attggcgacg agctgctgct gatgcgcatc ctcgaccagg ccttccccgg
     2581 ctgcgtgttt tacagcgaca acaccggcgg acgcttccgg atgatgatgc agcgtgaagg
     2641 gctaagcaga ccgctggagc cggcggaatt ctccgacggc actctgcgtt ttctgtgcct
     2701 ggcggtggcg ctattaagcc cgcgtccacc ggcctttatc gccctcaacg aaccggaaaa
     2761 cagcctgcat ccgcagatgc tgcccgcgct ggcgagctta atcgccgagg ccagccgcta
     2821 ctcgcaaatt tggctgacca gccactcgcc ggaactggcg aatttaattg agaaacagag
     2881 atcgttttcg ctgtatcagc tgtcgatggt ggaaggagaa acgaaggtgg agaggctggg
     2941 gtagtctccc ag
//
LOCUS       DADLDT020000251         2951 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000251 DADLDT020000000
VERSION     DADLDT020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.257"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JQH22_004228"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JQH22_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFB2208325.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH22_004229"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH22_004229"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFB2208326.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="JQH22_004230"
     CDS             2603..>2951
                     /locus_tag="JQH22_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFB2208327.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DADLDT020000252         2926 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000252 DADLDT020000000
VERSION     DADLDT020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.258"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="JQH22_004231"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="JQH22_004231"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFB2208328.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="JQH22_004232"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="JQH22_004232"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFB2208329.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="JQH22_004233"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="JQH22_004233"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFB2208330.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="JQH22_004234"
     CDS             complement(1896..2810)
                     /locus_tag="JQH22_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFB2208331.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          771 a          678 c          781 g          696 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct agaaccacgg cagcctgacg gctgcaaatt gaaaac
//
LOCUS       DADLDT020000253         2895 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000253 DADLDT020000000
VERSION     DADLDT020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.259"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(2..77)
                     /locus_tag="JQH22_004235"
     tRNA            complement(2..77)
                     /locus_tag="JQH22_004235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Val,seq:tac)
     gene            complement(122..197)
                     /locus_tag="JQH22_004236"
     tRNA            complement(122..197)
                     /locus_tag="JQH22_004236"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Val,seq:tac)
     gene            456..1871
                     /gene="gltX"
                     /locus_tag="JQH22_004237"
     CDS             456..1871
                     /gene="gltX"
                     /locus_tag="JQH22_004237"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFB2208332.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1923..2315)
                     /locus_tag="JQH22_004238"
     CDS             complement(1923..2315)
                     /locus_tag="JQH22_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HFB2208333.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2317..2676)
                     /locus_tag="JQH22_004239"
     CDS             complement(2317..2676)
                     /locus_tag="JQH22_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HFB2208334.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          707 a          708 c          728 g          752 t
ORIGIN      
        1 gtggtgggtg atgacggtat cgaaccgccg accccctcct tgtaagggag gtgctctccc
       61 agctgagcta atcacccact tctcaatttc ttgctacacg gcggagacta cataaagtag
      121 ttggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
      181 agctgagcta atcacccccg ctgtgtggag tcgcattata gggagagttc aaaatgagtc
      241 aacgcatttt ctaaagaaat tgttcgttcg tcgtaaattt aagcaagatg atcgcaaaac
      301 agaccgtgtt gcgcaatttg tcaacgaaaa caataatgcg taaggtagaa acccgaacta
      361 cattgaggaa tcaggcggga gtgatagaat atcgcccact caatttttcc aggatttgcc
      421 ggttgtcggc atctttctaa acgtaaggcc atttcatgaa aatcaaaact cgcttcgcgc
      481 caagcccaac aggctatctg cacgttggcg gcgcgcgtac tgctctttac tcctggcttt
      541 ttgcacgtaa ccacggcggt gagttcgtgc tgcgtattga agacaccgat cttgagcgtt
      601 ccacgccgga agctatcgaa gccattatgg atggcatgaa ctggctgagc ctggagtggg
      661 atgaaggtcc gtactaccag accaaacgtt ttgatcgcta caacgcggtg atcgatcaga
      721 tgctggaaga gggcactgct tataaatgct attgctctaa agagcgcctg gaagcgctgc
      781 gcgaagagca aatggcgaaa ggtgagaagc cgcgttatga cggtcgctgc cgccacagcc
      841 atgagcatca tgctgatgat gaaccgtgtg ttgtacgttt tgctaacccg caggaaggtt
      901 ctgttgtttt tgacgatcag atccgtggtc cgatcgagtt cagcaaccag gaactggacg
      961 atcttattat ccgccgtacc gatggttccc caacctataa cttctgtgtg gttgtcgatg
     1021 actgggatat ggaaatcacc cacgttatcc gtggcgaaga ccatatcaac aacacgccgc
     1081 gccagatcaa cattcttaag gccctgaaag cgccggtgcc ggtttacgcg cacgtttcta
     1141 tgatcaatgg cgatgacggt aaaaaactgt ccaaacgtca cggggcagtc agcgtaatgc
     1201 agtatcgtga tgacggttat ttgccagaag cactgctgaa ctatctggtg cgtctgggct
     1261 ggtcccacgg cgatcaggaa atcttcactc gtgaagagat gatcaaatac ttcactttga
     1321 atgccgtcag caaatctgcc agtgcgttca acaccgacaa gctgctgtgg ctgaaccatc
     1381 actacattaa cgcgctgccg ccggagtatg ttgctactca cttacagtgg cacattgagc
     1441 aggaaaatat cgatacccgt aacggcccgc agctggctga tctggtgaaa ctgctgggcg
     1501 aacgctgcaa gacgctgaaa gagatggcac agagctgccg ttatttctac gaagattttg
     1561 ctgagttcga tgccgatgcg gcgaaaaaac atctgcgtcc ggtagcgcgt cagccgctgg
     1621 aagtggttcg tgacaaactg gccgcgatta ctgactggac cgctgaaaac gttcatcacg
     1681 ctattcaggc gacggcggat gagctggaag tgggtatggg taaagttggt atgccgctgc
     1741 gtgtcgccgt aaccggtgcg gggcagtctc cagcactgga tgttaccgtt cacgcaattg
     1801 gtaagacccg cagtatcgag cgtatcaaca aagcgctgga ttttattgct gaacgcgaaa
     1861 atcagcagta attagcgtgt aaaaagataa acggcaggag ataatatcct gccgtttttt
     1921 atttatgctg attcgtcaat atcgagacgg gcgaggaaat tgcgaattcc ttcacgtgac
     1981 aaaaattgcg cgactttttc ctgctcaatg tctgtcatat tatctatcgc gttgatgatt
     2041 tgtcgatacg ccggggcgcg tgctccagga gcgtattcag caattctctc ttcagcttcc
     2101 atcaacattg gtgtgttatg gaaggctggc gtgttctgaa taaactcgca aacttgcgta
     2161 tcgaccagaa taagtctggc acgaccgcct ttcacgccgg gaaatttttc cgttttccag
     2221 cctttttctc tggtccaacg gttaacagtt tgtttagcca caccgaggca ttcagccagt
     2281 tcttcggtgg tcattttatt gcgtaatctt ttcatattat ttgctatcgc gaatccctaa
     2341 gcgttgtaac aatccgataa tcccttcccg cagaagcaag gatgtaaact gtttttgttc
     2401 aactggcgtc atctctttcg ccagtgtcac cagcaatact tcaagcggcg catcaccaga
     2461 aagggcaggc gcttctccct gaccttctgg acgttcagca ttgcgaatat attcacgaac
     2521 ctgttcattg acgtgaacca gtcgggcttt gccaccctgg acgccaggtt ttggtgacgt
     2581 tgtccagcct tccttgcgta cccatttatt aatggtctgg cggctatagc cggtcagtct
     2641 ggcaagttca tctggtgtca tcctctcctt gaacataaca attccctgaa tgtatgtggg
     2701 tctattagta gttcatttta taacaccact ttacaggaaa ccgtgacatg aataactact
     2761 gaatgcgagc ggttttccaa aattgttcat ttgcctgatt tttcagcgat tgaaactaca
     2821 cgttgcaatt tgccgttgac acattcgggc ggaattcata tgatgccgcc cgtcaactcg
     2881 acaagcttta cgtga
//
LOCUS       DADLDT020000254         2716 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000254 DADLDT020000000
VERSION     DADLDT020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.260"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            6..224
                     /locus_tag="JQH22_004240"
     CDS             6..224
                     /locus_tag="JQH22_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFB2208335.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="JQH22_004241"
     CDS             226..591
                     /locus_tag="JQH22_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFB2208336.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="JQH22_004242"
     CDS             588..1253
                     /locus_tag="JQH22_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208337.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="JQH22_004243"
     CDS             1253..1618
                     /locus_tag="JQH22_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFB2208338.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="JQH22_004244"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="JQH22_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFB2208339.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          773 a          558 c          697 g          688 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgttag
//
LOCUS       DADLDT020000255         2690 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000255 DADLDT020000000
VERSION     DADLDT020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.261"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            230..769
                     /locus_tag="JQH22_004245"
     CDS             230..769
                     /locus_tag="JQH22_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFB2208340.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="JQH22_004246"
     CDS             complement(995..1318)
                     /locus_tag="JQH22_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HFB2208341.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="JQH22_004247"
     CDS             1604..1798
                     /locus_tag="JQH22_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFB2208342.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          578 g          590 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgcctttcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DADLDT020000256         2690 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000256 DADLDT020000000
VERSION     DADLDT020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.262"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..213
                     /locus_tag="JQH22_004248"
     CDS             <1..213
                     /locus_tag="JQH22_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208343.1"
                     /translation="CADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            261..690
                     /locus_tag="JQH22_004249"
                     /pseudo
     CDS             261..690
                     /locus_tag="JQH22_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            877..1114
                     /locus_tag="JQH22_004250"
                     /pseudo
     CDS             877..1114
                     /locus_tag="JQH22_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1535..2113
                     /locus_tag="JQH22_004251"
     CDS             1535..2113
                     /locus_tag="JQH22_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208344.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2222..2480)
                     /locus_tag="JQH22_004252"
                     /pseudo
     CDS             complement(2222..2480)
                     /locus_tag="JQH22_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2464..2690
                     /locus_tag="JQH22_004253"
                     /pseudo
     CDS             2464..2690
                     /locus_tag="JQH22_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          806 a          496 c          698 g          690 t
ORIGIN      
        1 tgcgcagacc tgaatattcc gttctttgtg gatgccggaa acaaaaaact ggtcttctgg
       61 tttgtacgtg ttgatgacga agggtatccg gaaattgccc gctgcatgga gcgggaattt
      121 gcgacaattc ctgccggtat caacgccgac ggcatgtatt gccctgaatg cggtacggtt
      181 cactggccgg atggcgttat tccgcctttc tgatgcttcc ccgttttgcc gatatttttc
      241 agcagggaaa ccgatggctt aactggctgg agaagcagcc ggtgcagatg agccgcctgg
      301 aacattatgc cggtcaggat gaaattggcc tgcgttacaa cagccaccgg acaaaacgag
      361 aagaaaacct tgtgatgagt ggcgacgagt ttatggaaag gttctcctgg catgtggcgg
      421 ataaggggtt ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa
      481 gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga
      541 gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag
      601 atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat gatgcatgag
      661 ccgctggctc gaatacggtg gtgcggctga gagtcgcaga ggggaagttg cgtccatgtt
      721 gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt aagcaatatt
      781 aagtgtctat accgattact tgatgatcag gttgtgttga ataaacgcgc attcttcggt
      841 gcttttttaa tcttttgggg atttaaattc ctatcgatga actgtcgata tgaaaaagcg
      901 agcatcatac tgacgtcaaa caaaggggtc gcagactggg gagaaatgtt cggagatcac
      961 gtgctggcaa cagcgatcct agatcggctg ctacacgctg aatatcaaag gagagagtta
     1021 ccggttaaaa gagaaacgta aagctggagt gctgaccaaa aacacaacgc caatcagtga
     1081 tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag tatcttagca ggcatgaaaa
     1141 tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg aaggagggag aatgccatct
     1201 catcacttat atctggcgaa tgaaacacag atggtcggcc ttgcccccaa acacacggcg
     1261 gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga aatatgatta aatcagtgtt
     1321 atgttcgaac ctgacgtgtt aacagcagta aagtagtctc gcgaaggtgt tcaaacatta
     1381 taatgaatgg catcctcata gtgcgcaggg ttatcgcttg ctgcggaaat atctgaagcg
     1441 acgtaccagt aatggggaaa atgatagctg gtttctgaaa atatagagga aaatgtgata
     1501 tatatatcct gttaatattt aaggagaata taaaatgaaa gtctcgttta agtctttagg
     1561 ctatattttt catgatatat ataataaaaa acacactatc gatgagttca acgatgtggt
     1621 aagaaaagca gttttatcag gtaaaattaa cgagctgaat gcatgtcata aagtggcaat
     1681 ttttcttgcc gaaaaagaca atgagataac aaagaaagac aaagccaaga taatagatac
     1741 attaactgaa aactattcca ttgaatttca acaattaatg aatattagtg agcgcacgtt
     1801 aaattcatct ttatatatta ctcctggtga aagtggtttt gtcagttttg ttaatcgtga
     1861 aggaaaaata tgtcatactg catatgtaaa atcatcagat aatagcatga catattacca
     1921 tgcaaatggt agcagcattg acaaatatat tacggatatg tgcggcctta tttgtatgag
     1981 gcatattgag tctactggca ttatttttta tatgcttgat gaaaaagttt tatctgccat
     2041 tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt tgttcagcaa agaacttata
     2101 taaatgtgtt taattttaga aaaactattt ggtaaaatcc aacaagagtc gagaggttcg
     2161 cgttgcttca gcagggcctc agcctgttca accagactca gttgcaggtc gtgacgcaga
     2221 tttagtggtg gtggttgatt gtcagatacc agccgatgat cttgtagtgc atatcatcag
     2281 actttgagaa gcatatggtt ttgcgggtaa gccgcttaag atgtgtccgc aggttcagat
     2341 tatgtcgctc tatccgctga gtgcatttct tgcttataac atgactggtt gaggacaata
     2401 aagttcggta taccggctca ggcatcggtc atgtagaagg caatgctaaa ctggctcagc
     2461 aaactaagag cagcgcgcat ccacttaccc aatgcggttg agaaaatagc cttcgatcgc
     2521 tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa cccgccagaa tgaacatccg
     2581 catcttcctg ttgaaagtcg tcgtcaggca aagggaactc gcttcctgtg gcagcacagc
     2641 gataaatgga tgaccgaatc caggcaggaa aagctgatat ggctgcgtgc
//
LOCUS       DADLDT020000257         2572 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000257 DADLDT020000000
VERSION     DADLDT020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.263"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(64..822)
                     /locus_tag="JQH22_004254"
     CDS             complement(64..822)
                     /locus_tag="JQH22_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFB2208345.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JQH22_004255"
     CDS             complement(830..1933)
                     /locus_tag="JQH22_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFB2208346.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="JQH22_004256"
     CDS             complement(1943..>2572)
                     /locus_tag="JQH22_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFB2208347.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          710 a          682 c          602 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DADLDT020000258         2509 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000258 DADLDT020000000
VERSION     DADLDT020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.264"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..177)
                     /locus_tag="JQH22_004257"
     CDS             complement(<1..177)
                     /locus_tag="JQH22_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFB2208348.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="JQH22_004258"
     CDS             complement(380..847)
                     /locus_tag="JQH22_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFB2208349.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="JQH22_004259"
     CDS             complement(844..1377)
                     /locus_tag="JQH22_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFB2208350.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="JQH22_004260"
     CDS             complement(1443..2072)
                     /locus_tag="JQH22_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208351.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="JQH22_004261"
     CDS             complement(2076..2291)
                     /locus_tag="JQH22_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFB2208352.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          630 a          656 c          565 g          658 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg caccgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaa
//
LOCUS       DADLDT020000259         2471 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000259 DADLDT020000000
VERSION     DADLDT020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.265"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..568
                     /locus_tag="JQH22_004262"
     CDS             <1..568
                     /locus_tag="JQH22_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFB2208353.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="JQH22_004263"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="JQH22_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFB2208354.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="JQH22_004264"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="JQH22_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFB2208355.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="JQH22_004265"
     CDS             2170..2406
                     /locus_tag="JQH22_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFB2208356.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          633 a          542 c          640 g          656 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttatcca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgcagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga taattggtgc gagggggggg
     2461 acttgaaccc c
//
LOCUS       DADLDT020000260         2432 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000260 DADLDT020000000
VERSION     DADLDT020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.266"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            65..1543
                     /locus_tag="JQH22_004266"
                     /pseudo
     CDS             65..1543
                     /locus_tag="JQH22_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="JQH22_004267"
     CDS             1540..>2432
                     /locus_tag="JQH22_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFB2208357.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DADLDT020000261         2390 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000261 DADLDT020000000
VERSION     DADLDT020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.267"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..107
                     /locus_tag="JQH22_004268"
     CDS             <1..107
                     /locus_tag="JQH22_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFB2208358.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="JQH22_004269"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="JQH22_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFB2208359.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="JQH22_004270"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="JQH22_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFB2208360.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2390
                     /locus_tag="JQH22_004271"
     CDS             2210..>2390
                     /locus_tag="JQH22_004271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HFB2208361.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          653 c          673 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DADLDT020000262         2378 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000262 DADLDT020000000
VERSION     DADLDT020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.268"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="JQH22_004272"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="JQH22_004272"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFB2208362.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2378
                     /locus_tag="JQH22_004273"
     CDS             1647..>2378
                     /locus_tag="JQH22_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFB2208363.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDA"
BASE COUNT          638 a          520 c          615 g          605 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgct
//
LOCUS       DADLDT020000263         2373 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000263 DADLDT020000000
VERSION     DADLDT020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.269"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..823)
                     /locus_tag="JQH22_004274"
     CDS             complement(<1..823)
                     /locus_tag="JQH22_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFB2208364.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JQH22_004275"
     CDS             complement(1424..1672)
                     /locus_tag="JQH22_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFB2208365.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JQH22_004276"
     CDS             complement(2027..>2373)
                     /locus_tag="JQH22_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFB2208366.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          577 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaaaacgc ataaaaatcc
     1681 ctctgcactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg tgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADLDT020000264         2311 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000264 DADLDT020000000
VERSION     DADLDT020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.270"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            47..268
                     /gene="kilR"
                     /locus_tag="JQH22_004277"
     CDS             47..268
                     /gene="kilR"
                     /locus_tag="JQH22_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFB2208367.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            262..438
                     /locus_tag="JQH22_004278"
     CDS             262..438
                     /locus_tag="JQH22_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208368.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            513..788
                     /locus_tag="JQH22_004279"
     CDS             513..788
                     /locus_tag="JQH22_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208369.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            888..>2311
                     /locus_tag="JQH22_004280"
     CDS             888..>2311
                     /locus_tag="JQH22_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFB2208370.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          711 a          531 c          528 g          541 t
ORIGIN      
        1 ctttcctgag atttcctttg taagactgtt tattaaggag gcttccatga ttgctcatca
       61 cttcggaact gatgaaatac cacgtcagtg tgtgactcct ggcgattatg ttcttcatga
      121 aggtcggaca tatatcgcct cggcaaacaa tattaaaaag cgaaaacttt atattcgtag
      181 cctgactaca aaaacatgca tttctgactg catgattaaa gtcttcctcg gtcgtgatgg
      241 tttacctgta aaggcggagt catggtaatg actaagaaaa taaaatgtgc ttatcacctt
      301 tgcaataaag aaattgaaga aagcaaaatc attacaagac cacttcattt catgcgtgga
      361 gttataccaa cgacggaaat gaaaaaatat tgtagtgaaa tctgtgccga aaaagaccag
      421 atggcacacg aactttaatt aactgactat ccgaaactga atttatgcca gcaatggcag
      481 ggattcgctc aaccttaatt aaggagaaaa acatgattac cagttatgaa gccactgttg
      541 taactactga tgacattgtt cacgaggtta atctggaagg aaagcgtatt ggctacgtga
      601 ttaagacaga aaataaagaa accccattca ctgtggttga tatcgacggt ccatcaggca
      661 acgttaaaac acttaacgat ggtgtaaaaa aaatgtgtct ggtgcacata ggaaagaatc
      721 tgcccgcaga aaaaaaagcc gaatttctgg caactctgat tgcaatgaaa ttaaaaggtg
      781 aaatctgaaa aaaagaaagc ctgcacactg tgcaggcctg aatgaagaac ctgggacatt
      841 tattcatcac tcgcagtaat tttaatctga gttgaggtta aaaaacaatg aacaccgata
      901 aacaagttta cccactgtat tacgaagcaa aaaatgacaa agtaagaaaa cgtctcggta
      961 ttaaaggcgg tttttactgg gctgaagcga aaaaattatc cattgccatc tcccgtggtg
     1021 ctgttgcgat tgacgatgca ggctacgatg aagatgactt taaaaaacct gttcgcgtca
     1081 atttgcccgt tgttgatgac ctcccgccag aaggcgtatt tgatacggaa ttctgcaacc
     1141 gttacgaaaa aggcggggaa gatggcatca caatggtatt tatcgcatct tctccctctg
     1201 ttcaggacaa accggccagc actgacaata ccaacgtcaa cggcgaagac atgacagaaa
     1261 ttgaggagaa catgctcctg ccagtttccg gtcaggaact gcccattcgc tggcttgcgc
     1321 aacacggcag cgaaaaacca gtaacgcacg tttcacgcga cgaactccag gcattacaca
     1381 ttgctcggga tgaagaacta ccagctgtta ctgccctggc tgtttcccac aaaaccagcc
     1441 tgctcgatcc gctggaaatt cgcgatctcc acaaactggt gcgtgatact gacagagttt
     1501 tccctaatcc aggcaattca agcctggggc tgatgactgc ttttttcgaa gcatacatgg
     1561 acgcagacta caccgatcgc ggtctgctga caaaagagtg gatgaaagga aatcgtgttt
     1621 cacgcatcac gcgcacggct tccggcgcta atgctggcgg cgggaacctc accgatcgcg
     1681 gcgaaggttt cgttcacgat ctgacgtcac tggcgcgcga cgtagccact ggcgtactgg
     1741 ctcgttcaat ggacgtggac atttataacc ttcatccggc acacgctaaa cgtgtcgagg
     1801 aaattatcgc tgaaaataaa ccgccctttt ctgttttccg cgacaaattc atcaccatgc
     1861 ctggcgggat ggattattcc cgcgccatcg tggttgcgtc agtgaaagaa gcaccgattg
     1921 gtatcgaggt catccccgct cacgtcactg aatatctgaa caaggtactg actgaaaccg
     1981 atcatgccaa ccctgatccg gaaatcgtgg atattgcctg cggtcgctcc tctgccccga
     2041 tgccgcaacg tgtaacagaa gaaggaaaac aggatgatga agaaaaaacg cagccatctg
     2101 gcgcaatggc agatgaacag gcaacggctg aaacaatgga accggatgca actgaacatc
     2161 atcagaacac gcagccgctg gatgctcagt cacaggtaaa ttctgttgat gagaaatatc
     2221 aggaactacg ggcagaactc catgaagccc ggaaaaacat tccgcccaaa aatcctgtcg
     2281 atgcagacaa attactggct gccttgaggt a
//
LOCUS       DADLDT020000265         2212 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000265 DADLDT020000000
VERSION     DADLDT020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.271"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(273..1727)
                     /gene="ospC4"
                     /locus_tag="JQH22_004281"
     CDS             complement(273..1727)
                     /gene="ospC4"
                     /locus_tag="JQH22_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFB2208371.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKVN
                     SGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          653 a          407 c          375 g          777 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactctggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tactttttga aattttttgt ttacatcaac caatccatta
      421 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      481 tcatacagat tacaatatct attgtttttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa
      601 taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat ctcttcataa
      661 ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt tttgaagtta
      721 tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat atttttgtta taacaaaact ctctgaatcc ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtgtta
      901 aatattggaa catcagtttt cccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtgatcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattgg cgccaaaatc tacagtatga tgtgttttat ttagtgggag tttttcttga
     1141 tgattggaaa tttcgacccc aaaaaaaaca aaatcgtggt tagagatttc tttaatatcc
     1201 tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc aaaggattta
     1261 atattaagaa tattatttga cagattagag ttactttgat gtctgaagtt aaaattagaa
     1321 ttaataataa tagaaagtaa gtgacgttcg tctgaagaaa gaggatgaac tttttgtatt
     1381 atctcagaga tatagtgcct ggacacctta tcgagatgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttagtgatt tgaaactggt cccttgagaa
     1501 aacattttgc tataactttg cgctgctata gacttcctta aaaagttttt cacagtatta
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagaag atgagtttgt tacgcgtaat
     1621 acattttttt gtaatgcttt tgttgcttta ttcggattcg tttttccatc aacaagatca
     1681 atattattct gaacattaat atgatttact gcttcaggta ttttcatccc cccccccaat
     1741 tatgtgcgtt tttatctgtg gttatttaga taacattatc taactggaca ctttcttgta
     1801 tgtattatag ctaaatgaac agattttaat atgttgtatg gttttcactc tgaaatcccc
     1861 tgaatttaat cacgtttcct ttactgaagg caattttaac cgtagtatca attattaacc
     1921 cttgggcttg atatccctct cctcttctgg gcacggggga tgtcagatta cttattacag
     1981 aaaccaaaag aattgtaagc gtcatctcag tgtcgtctat ttcaatgtct gatgattggc
     2041 gatgatagag tcctcttata acgttaatgg actctatcaa tgtcagattc tcttcgcccc
     2101 cgcaggattc gtgcctctta ctctatgact ctatggagtt taagcttgat cttattgaaa
     2161 aatcttacca gctaggggcc tgcgtggccc aactggccag agagtacggg at
//
LOCUS       DADLDT020000266         2201 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000266 DADLDT020000000
VERSION     DADLDT020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.272"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            19..2201
                     /locus_tag="JQH22_004282"
                     /pseudo
     CDS             19..2201
                     /locus_tag="JQH22_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          586 a          490 c          553 g          572 t
ORIGIN      
        1 gataaggata ggcgtattat gatgacgacc agaatagtgg ttggcctcac ggcagggacg
       61 tgtctgattt tctcgcaaaa cctgatggcc gaggtcagtg tattcaatcc ggcgcttctg
      121 gaaatcaacc atcaatccgg agtcgatatt cgccagttta atcgggcaaa cctgatgccc
      181 ccaggtgttt atagcgttga tatttttatc aacggtaaaa tgtttgaacg tcaggatgtg
      241 acatttgttc aggataatcc agatgctgat ctgcacgctt gctttattgc cattaaaaaa
      301 acactgtcct cctttggcat aaaagttgat gcgctcaaat cgttcaatga tgtggatgag
      361 acggtttgcc tcgatcctgc tccacgtatt gaaggctcat cctggcagtt tgacagtgat
      421 aaattgcagc tgaatatatc cattcaccaa atctacatgg acgcgatggc ttatgattac
      481 atcagcccca cgcgttggga tgaggggatt aatgcgctca ccatcaacta cgatttttct
      541 ggttcacata cactacgttc agattatggt tcacaagaga cagataccag ttatctcaat
      601 ctgcgcaatg gactgaatat tggaccgtgg cggctacgta attacagtac tttaaacacc
      661 agcgatggcc gtgcggaata caactccatt agtacctgga tacagcgcga tattgccgcg
      721 ttaagaagcc agattatgat tggtgatacg tggacggcga gcgatatttt cgacagtacg
      781 caaattcgcg gcgcgcgttt gtatactgat aacgatatgc tacccgccag ccagaatggc
      841 tttgctcctg tggttcgtgg gattgcaaag tccaacgcca ccgtcatcat tcggcagaat
      901 ggctacgtga tttatcagtc agccgttcca caaggtgctt ttgagatcac cgatctcaac
      961 accgcaagta caggtggcga tttggacgta accatcaaag aagaagacgg tagcgaacaa
     1021 cgattcaccc aaccttatgc ttcattggcg attcttaaac gtgaaggtct gacagatgtt
     1081 gatgtcagcg tgggtgaatt gcgcgatgaa gacggattta caccggacgt ccttcaggcg
     1141 caaatacttc atggtttttc ccacgggatc actttatatg gaggtatgca ggctgctgaa
     1201 aattatggtt ctgcagctct gggtgtcggt aaagatcttg gcgctttggg cgcaatttct
     1261 ttcgatgtga cacatgctcg tgcgaatttt agccatgatg atacagaaac gggtcagtca
     1321 tatcgctttc tctattcaaa actatttgac gacacagaca ctagcttgcg cctggttggc
     1381 tatcgttact ccaccgaggg ctactatacc actcaatgag tgggcatcgc ggcgcaacag
     1441 ccctgaagac ttttgggaaa caggtaaccg acgtagtcgc gtggagggaa cgctaacgca
     1501 gtcgttgggg agagattatg gcaatttata cctgacatta agccggcaac aatactggca
     1561 taccgatgat gtcgaacgat taatgcaatt tggctacagc agtagctgga agcgtctctc
     1621 gtggaacgtc tcctggagtt attccaatac tgccagacag gggacgggga acaaccatgc
     1681 cagtgataac accagtgagc agatctacat gctctcttta tctgttcctt tatcgggctg
     1741 gtggggtaat agttacgcca cctattctgt ttcgcaaaac gataattccg gtagctcaca
     1801 tcaactcgga ctcagcggta cggcgctgga aagaaataac ctttcatgga atttaatgca
     1861 gtcctataac agtcatgatg atgaggttgg cggtaatatg tccctgacct atgatggctc
     1921 ttatggcacg gtgaacggca gctataacta cagccaaaat tcccagaggc tgaattatgg
     1981 tatcagaggg ggaattctgg cacacagcga aggggtaacg ttaagtcagg agttaggtga
     2041 aactattgct cttgttaaag cacctggggc cgccgggtta gaaatagata atatgcgcgg
     2101 tgctgcgacg gactggcgcg gctatacggt caagacacag ctaaaccctt atgatgaaaa
     2161 tcgggtagca atcagcgata actatttctc gaagtcgaat a
//
LOCUS       DADLDT020000267         2187 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000267 DADLDT020000000
VERSION     DADLDT020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.273"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..698
                     /locus_tag="JQH22_004283"
     CDS             <1..698
                     /locus_tag="JQH22_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208372.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="JQH22_004284"
     CDS             700..1305
                     /locus_tag="JQH22_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFB2208373.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>2187
                     /locus_tag="JQH22_004285"
     CDS             1292..>2187
                     /locus_tag="JQH22_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208374.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          507 c          610 g          538 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg
     1981 cccgttcacc attaatttgc ggacgggtga aatatccatg tcgcataaag tgtctgttgg
     2041 cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa
     2101 ttcaattgtt cttggtgata atgacaccgg atttaaacag aatggagacg gtattctgga
     2161 tgtttatgct aatagtcagc gtgtatt
//
LOCUS       DADLDT020000268         2168 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000268 DADLDT020000000
VERSION     DADLDT020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.274"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            155..340
                     /locus_tag="JQH22_004286"
                     /pseudo
     CDS             155..340
                     /locus_tag="JQH22_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..649)
                     /locus_tag="JQH22_004287"
                     /pseudo
     CDS             complement(440..649)
                     /locus_tag="JQH22_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(699..878)
                     /locus_tag="JQH22_004288"
                     /pseudo
     CDS             complement(699..878)
                     /locus_tag="JQH22_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1402..1686
                     /locus_tag="JQH22_004289"
     CDS             1402..1686
                     /locus_tag="JQH22_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFB2208375.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            1686..1961
                     /locus_tag="JQH22_004290"
     CDS             1686..1961
                     /locus_tag="JQH22_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFB2208376.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          601 a          539 c          522 g          506 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt cagtaatttt atgtttttcc tgaaaaaatt actctttttc gaactccagt
      721 gagcgttcac gactcatcag tgtgaaaatg atggcaaaaa cagcagacag tatcagtacg
      781 ataaatgtgg ccccccatcc cagataatcg acaacaagtc ccattcccag tgtcgcaaca
      841 attgagccga agatatagga aaatgtgcct gacagcccta acagaataag tttacgcgta
      901 aatcgcccgg aatccagaag agcattaagt atcacataaa caggttccat accagtcctg
      961 accgcatcag gacgccagta aataaaatct cgtagtccac cggcatcaga gacaagagta
     1021 atcgtcgtta ccggtatttc gactggtttg tactggtgtg agcggacaaa actactgtac
     1081 gtctctcata cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     1141 ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac agaggcattt tcatcgctga
     1201 caggccgcat ggcctcgcag agccggaggg ggactggtga tattcggacg accgtaccgg
     1261 cttgcgccgg gcttcggcgg ttttacacct gtgctacatg taaccaccgt atgccgcctt
     1321 atatgcgctg actcgcatta tttgccttgt tccgtctgaa agtaatcact atgattaaat
     1381 atgattaaca actaatcggg tatgcaaatg aaaaacaata ccgcacaagc aacaaaagta
     1441 attaccgcgc atgtgccatt acctatggct gataaagtcg accagatggc cgccagactg
     1501 gaacgctccc ggggctgggt tatcaaacag gcgctttctg catggcttgc ccaagaggag
     1561 gagcgtaatc gcctgacgct ggaagccctg gacgatgtga catccggaca ggttatcgac
     1621 catcaggctg tacagtcctg ggcggacagc ctcagtactg accatccgtt accggtgcca
     1681 cgctgatgga actgaagtgg accagtaagg cactttctga tttggcgcgg ttatatgatt
     1741 ttctggtgct ggccagtaaa cctgctgccg ccagaacggt gcagtccctg acacaggcgc
     1801 cggtcattct gttaactcat ccacgtatgg gagaacagtt gtttcagttt gaacccaggg
     1861 aggtcagacg gatttttact ggcgagtacg aaatccgtta cgaacttact ggccagacta
     1921 tttatgtatt gcgcctgtgg cacacacgag agaacaggta gattgggtgt ggaggcaact
     1981 ttaacatagc ctgcaggtat taattctgga gcattgttat caggccagat attttgcatt
     2041 ccattaaata atcttgtagg agataaaata aattacgatg aaataacgaa aattaccgct
     2101 cgtgactggc ggcaatatcg cgctccgggc tggcaaataa cccatcagaa acgttattgc
     2161 cagacatt
//
LOCUS       DADLDT020000269         2134 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000269 DADLDT020000000
VERSION     DADLDT020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.275"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..241
                     /locus_tag="JQH22_004291"
     CDS             <1..241
                     /locus_tag="JQH22_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208377.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="JQH22_004292"
     CDS             complement(238..1179)
                     /locus_tag="JQH22_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFB2208378.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="JQH22_004293"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="JQH22_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..>2134)
                     /locus_tag="JQH22_004294"
     CDS             complement(1684..>2134)
                     /locus_tag="JQH22_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208379.1"
                     /translation="DDDLKTLLKALLAKLERNATGDNDNKPDDNPTGDGEDDEEETTI
                     TGDSAWRAEVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDM
                     AFNAVSEVAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          461 a          591 c          545 g          537 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atca
//
LOCUS       DADLDT020000270         2124 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000270 DADLDT020000000
VERSION     DADLDT020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.276"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            42..221
                     /locus_tag="JQH22_004295"
     CDS             42..221
                     /locus_tag="JQH22_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208380.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            273..467
                     /locus_tag="JQH22_004296"
     CDS             273..467
                     /locus_tag="JQH22_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208381.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1266..1602)
                     /locus_tag="JQH22_004297"
                     /pseudo
     CDS             complement(1266..1602)
                     /locus_tag="JQH22_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          683 a          436 c          436 g          569 t
ORIGIN      
        1 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
       61 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      121 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      181 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      241 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      301 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      361 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
      421 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
      481 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac
      541 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
      601 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacga tttaatgtgt
      661 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
      721 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
      781 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
      841 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
      901 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
      961 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1021 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1081 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1141 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1201 gaatatgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1261 ttacactata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1321 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1381 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     1441 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     1501 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     1561 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     1621 tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca
     1681 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     1741 attattataa taacggcttt aattggaata catatattac aacgtattat atataattgg
     1801 tattctggga actatattct caaaatatag tagaaacgag gtatgtttct ggtggaaagg
     1861 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
     1921 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
     1981 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
     2041 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
     2101 aacacaatat tcatagtatg gcga
//
LOCUS       DADLDT020000271         2123 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000271 DADLDT020000000
VERSION     DADLDT020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.277"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(33..299)
                     /gene="ospE2"
                     /locus_tag="JQH22_004298"
     CDS             complement(33..299)
                     /gene="ospE2"
                     /locus_tag="JQH22_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFB2208382.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(481..882)
                     /locus_tag="JQH22_004299"
                     /pseudo
     CDS             complement(481..882)
                     /locus_tag="JQH22_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1311..2099
                     /gene="virF"
                     /locus_tag="JQH22_004300"
     CDS             1311..2099
                     /gene="virF"
                     /locus_tag="JQH22_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFB2208383.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          654 a          378 c          395 g          696 t
ORIGIN      
        1 aaaatggagc ttaaacgagt ctgaaattaa cttcagaaat cagaataccg ttggctttgt
       61 ttttcatcaa ttccggcttc atttatcttt gtcaaccatt gcatttctgt agcagtgaat
      121 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
      181 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      241 tcaagaatga tattttcttg tttttgtgga aggcaaggga atatagtttg tgtaagcata
      301 ttgcttcctt atgttatatt ttagtaaaga atttattttt tacattatag tcggctaaat
      361 gaagtttact gtaaaaaaac gatacttttt tcgtaagcga tgagatgtgg agccagaaaa
      421 atggctgcgt tacgtcattg aactgtcctt gacccacgat attcagacag tcgttgtatc
      481 ttaagttgac attacccgct gacgtcgata ttcccttggt gagagataac ccaacgcact
      541 atgcggatgg ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct
      601 tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat
      661 tccattactc tgagggctac tgactgccgt cgtgcagggc tccagattca actctctggc
      721 gaactgccgt gtttcatgag ccgtataagc cgaaccgtta tcggccactg gattggccgt
      781 tccggtcctt tatcgccata tcgctcctga catgacatcc tgcaccgttg ctttgtcata
      841 atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa cgtaacgctg
      901 tcatcgggag aatcgtcaat gcggcattcg gttaacgctt accaatatca acaccaaccg
      961 gatttgtgga gcctccagtc tgaaggggct ttatgcgttc cgtataggat attatgatgc
     1021 tggagttttg cgaagctcca ctcctttccg aacatttaca cagatattgc ctaagaaaag
     1081 tgaaaccata atatggttat agtccctttc agtgcaaata cttagcttgt tgcacagaga
     1141 aatagaagct gcataagctc tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa
     1201 ttacttaatc tatcttaata acggaaagat tttgtataca atcactgtta cagaaatttc
     1261 ttagttactc tgtaaacact aaatatagtt tggtatattc tgttgaattt atgatggata
     1321 tgggacataa aaacaaaata gatataaagg ttcgcttgca taactatatt attttatatg
     1381 caaaaaggtg ttcaatgacg gttagctcag gcaatgaaac tttgactatc gatgaagggc
     1441 aaattgcttt tatagagcga aatatacaaa taaacgtctc cataaaaaaa tctgatagca
     1501 ttaatccatt tgagattata agccttgaca gaaatttatt attaagcatt attagaataa
     1561 tggaaccaat ttattcattt caacactcct attctgagga gaaaaggggg ttaaacaaaa
     1621 aaatattcct cctctctgag gaggaggttt ctatcgattt gttcaaatct ataaaagaga
     1681 tgcctttcgg caaaagaaag atctatagtt tagcttgcct tttatcagct gtttctgatg
     1741 aggaagcttt atatacttcg atatcgatag cttcttctct tagtttttct gatcagataa
     1801 ggaagattgt tgaaaaaaac atcgagaaga gatggcgtct ttctgatatt tcaaataact
     1861 tgaatttatc agaaatagct gttagaaaac gattggagag tgaaaaatta acatttcaac
     1921 aaatccttct tgatattcgc atgcatcatg cagcaaagct tttattgaat agtcaaagct
     1981 atattaatga tgtatcaaga cttatcggaa tatcaagccc atcttatttt ataaggaaat
     2041 ttaatgaata ttatggtata actccaaaga aattttactt atatcataaa aaattttaaa
     2101 tgcttcatag cccatcgcta ttg
//
LOCUS       DADLDT020000272         2122 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000272 DADLDT020000000
VERSION     DADLDT020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.278"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..550
                     /gene="traX"
                     /locus_tag="JQH22_004301"
     CDS             <1..550
                     /gene="traX"
                     /locus_tag="JQH22_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFB2208384.1"
                     /translation="AFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFP
                     WYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLMLAVSH
                     RLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKSLPRF
                     WPGDFFPVFYACHLAVLGVLAL"
     gene            605..1165
                     /locus_tag="JQH22_004302"
     CDS             605..1165
                     /locus_tag="JQH22_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFB2208385.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1297..1509
                     /locus_tag="JQH22_004303"
     CDS             1297..1509
                     /locus_tag="JQH22_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFB2208386.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            1755..>2122
                     /locus_tag="JQH22_004304"
     CDS             1755..>2122
                     /locus_tag="JQH22_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFB2208387.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVL"
BASE COUNT          512 a          474 c          651 g          485 t
ORIGIN      
        1 agcctttccg ctgtttgccc tggtgtgggg gctgaatctg tcccgtcatg cgcatatccg
       61 gcaaccggcc attaaccggc tgtggggatg ggggattatt gcccagttcg catattatct
      121 ggcaggcttt ccctggtatg aggggaatat cctgtttgcc tttgcagtgg cggcacaggt
      181 gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca gcggccattc ttctgatggc
      241 cctgtggggg cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt
      301 cagccaccgg ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgtct
      361 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggctggtct
      421 tgtgatgacg gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt
      481 ctggcccggg gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct
      541 ggcgctgtga cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca
      601 cagcatgaca gagcagaagc gaccggtact gacactgaag cgaaaaacgg aaggggagac
      661 gcctgtccgc aggcggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
      721 aaagcagaag ctggccgaga aagcagcccg ggaagcggag ctggcggcaa aaaaagcgca
      781 ggccagacag gcgctgtcca tttatctgaa cctgccctcg ctggatgagg ccgtgaacac
      841 cctgaaaccc tggtggccgg gattatttga cggtgaaacg ccccggcttc tggcctgcgg
      901 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
      961 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     1021 cggtgcctgc cggtatgata cggaagggta tgtgacggag catatttctc aggaggagga
     1081 agcgtatgcg gcagcgcgtc tggataaaat ccgccgccag aacaggataa aggcagagct
     1141 tcaggccgtg cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata
     1201 aggcttaaaa ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac
     1261 accataatac tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca
     1321 cggaacggtc aggtctggcg ctggcagcgt gccggaagcc gggccgttat tctggaggtc
     1381 agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcgccccgg
     1441 acagactggc agcagtttgc ccgaaaaagg gctgaacact gccaccggcg cagccgggga
     1501 agggtgtaac gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca
     1561 tgagatttgc tttttttaat atccggtcgg gcagtatgac cggacagttc tgagtagcac
     1621 agagtttttt ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg
     1681 tgttattcag tgtgtgctgc gatattcagg attacctgaa atacaggtgc catttatctg
     1741 atatggagaa taacatgagg aaatatattc cactggtatt atttatcttt tcatggccgg
     1801 tattgtgtgc agatattcat ggacgggttg ttcgtgtact ggacggtgat accatcgagg
     1861 tcatggactc acggaaggca gtgcggatca ggctggtaaa tattgatgca ccggaaaaga
     1921 aacaggatta tggccggtgg tcaacggaca tgatgaaatc actggttgca ggaaaaacgg
     1981 ttaccgtcac atattttcag cgagatcgtt atggtcgtat tctcgggcag gtttatgcac
     2041 cagacggaat gaatgttaat cagtttatgg tccgggccgg ggctgcctgg gtatatgagc
     2101 agtacaacac agatcccgtg ct
//
LOCUS       DADLDT020000273         2100 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000273 DADLDT020000000
VERSION     DADLDT020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.279"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="JQH22_004305"
     CDS             complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="JQH22_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFB2208388.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLST"
     gene            1044..1223
                     /locus_tag="JQH22_004306"
                     /pseudo
     CDS             1044..1223
                     /locus_tag="JQH22_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1240..1931)
                     /locus_tag="JQH22_004307"
     CDS             complement(join(1240..1685,1685..1931))
                     /locus_tag="JQH22_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFB2208389.1"
                     /translation="MTTVTVHYPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGCYTARTLKIGINTVIRTFKKLSPRRITSAPVAHADVA
                     LICELDEQWRFVGSKAQQHWPWYAYNTKTGGVLAYTCGPRNDETCRELLALLTPFCIG
                     MVTSDDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHE
                     KVIGSFIEKHIF"
BASE COUNT          621 a          384 c          471 g          624 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatattaa tcctgtaact tgaatctaat tgtgaaaaac
       61 tctccggaag agtggttatc tgattaaaat gaaaataata ttccttttgt ctgatattgt
      121 ttctatcaga aaattgagga aaagtaacca gattattgcg ctgagcacag agaatttcca
      181 gtttatccgg taagtcgggt aatgttgtca gcctgttata ataaacatgt aaaaatttca
      241 atttttcggg aagagccggt aagttatata gcctgttatt ttcaacaaat aatgatgtta
      301 aatggtttgg caaaacagga aggttttcca gttgattaga acgaacactt aaagttgtta
      361 atgactcagg aagttctggt atcacagaaa gcctgttatt atcagcatta agttctttaa
      421 gaaatgcagg cagctttggc aatgatgtta atttattatt ttctacaatc aaagcagaaa
      481 tatgctttgg taattcggga agagacgtca aatttaggtc tgataaattt aatactgctt
      541 cttgattctg aaagcactgg gagagtctgt taaaagcaat gcctcgctct tccccgggag
      601 aagagttctt ttcccattca gtcaggattc tcagataatg ttcattactg gttgagtttg
      661 agttaataga gggggagcaa gaaactgatg agtgtgtatt atttacagag aacataatgc
      721 taggtcaacc ttatatttgt gagaataaat atttaaaatt gtatatttat gcaaatttca
      781 cttaccattt ccgatattaa gttttaatag tttccatttt acacataaca gaagttagtt
      841 gtaatttatt tatggaggtt ttctatggaa ttcttttttt attgtgactg cagtcaataa
      901 taacactgaa ttcaacattc cagtgagcaa ggaaatgtaa tccgagtaag tatatttgtt
      961 ttacatcata ttttagtcaa gctcatttat tcctattata gaatgacaaa ttaaaattac
     1021 tgtaaaaaaa cgatacacat ttaatacgag ggaggccggc agaaaaacgg cttgcggtca
     1081 ggcaggagaa atgtgtgccg cgaatgaagt ccttgtatga ctggataaag ctccagagga
     1141 aaactttgtc aaaccatgct gaaatggtaa aggcgttcaa ctatatcctg aattactgga
     1201 atgcactgaa tgagttttgc cgggtagtac ttccaatcat tagaatatgt gcttttcaat
     1261 gaaggaaccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     1321 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgtgcaatac gttgcgtgaa
     1381 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     1441 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     1501 atcattccgt ggcccgcaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     1561 ccagggccag tgttgttgcg ccttgctgcc aacgaagcgc cactgctcat caagttcaca
     1621 gataagggca acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgagagctt
     1681 tttaatgtcc gtatgacggt attgatgccg attttcagtg ttctggcggt ataacagccc
     1741 cctgcgccgt tatgtgccat ttcaacgatc tgctccttaa cgccgagttt tctggcttca
     1801 taagagtaag tgagctggaa aacacgttta catgagcgac aacggaaacg ctcatgccgg
     1861 gaacaactac ggccatgtcg ataaacttca tcggaattac agcgcggata atgaacagta
     1921 acggtagtca tggaaaacct caaataccag tagattacca gaaacttcta tcgattgaag
     1981 acattacctt ttgccgcaat aggtggggta acattggtga aaacgcatta cgccgggtgg
     2041 cagttggaaa aaaattatct tttttcggct ctgatcgtgg tggagaaaat gcggcaataa
//
LOCUS       DADLDT020000274         2096 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000274 DADLDT020000000
VERSION     DADLDT020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.280"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="JQH22_004308"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="JQH22_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFB2208390.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="JQH22_004309"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="JQH22_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFB2208391.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DADLDT020000275         2084 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000275 DADLDT020000000
VERSION     DADLDT020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.281"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..796
                     /locus_tag="JQH22_004310"
                     /pseudo
     CDS             1..796
                     /locus_tag="JQH22_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="JQH22_004311"
                     /pseudo
     CDS             911..1252
                     /locus_tag="JQH22_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="JQH22_004312"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="JQH22_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          504 c          631 g          433 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatccag gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DADLDT020000276         2046 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000276 DADLDT020000000
VERSION     DADLDT020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.282"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(57..326)
                     /locus_tag="JQH22_004313"
     CDS             complement(57..326)
                     /locus_tag="JQH22_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFB2208392.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..1051
                     /locus_tag="JQH22_004314"
     CDS             383..1051
                     /locus_tag="JQH22_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFB2208393.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1367..>2046
                     /locus_tag="JQH22_004315"
     CDS             1367..>2046
                     /locus_tag="JQH22_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFB2208394.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          381 g          602 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
      541 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
      601 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag
      661 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc
      721 ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
      781 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg
      841 aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa
      901 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
      961 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt
     1021 agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag
     1081 acgtgctacg gtttccttta cgatttgggg ttgcgacttt acctctattg ataatgcatt
     1141 ccggcagaac gttcaaatat gaacgtacga tatttaacta accgaaaaac aaaatatagc
     1201 acaggcgaga catttaccat ctaagaaaat agtatcgttt ttttacagtt aaatcaacat
     1261 cacttcctta aaatgaaaac aataaatttc aatcctgaaa cattttattt cagcttatta
     1321 ttccccagta tcacatttta tcaggatatc cttctgcgag gttattatgc ttcctgtaaa
     1381 taatcccccc ctatccactg gaaacgtctc tttttacaga actacatcaa tcgacaatgt
     1441 tcacaataat tatctctccg aatgggttga atggactaaa aacagcattt ccggagaaaa
     1501 cagggaaact gcttttaccc ggctccaatt atgtctggag aacagtgaaa catcgttgga
     1561 cttatcttgt ttaggtctca gatctctacc acgattgcct gacaatcttg atgaaattaa
     1621 tgtaagcaat aaccaactat caatgctccc cgagctacca agggcattga aagagctgaa
     1681 tgcaagcagt aatcaattat ctgcacttcc tgaattacca gtgtcgctgg aatatataaa
     1741 tgtgagtgat aaccatttgt tcgcacttcc tgaattacct gcgtcactag aatatattaa
     1801 tgtaagtgac aatcacctgt ctgtacttcc gaggttacca atgtcattgg aattacttga
     1861 tgcagccaga aatgctttgg aagtaatacc agattttcca gaaagagatg atcatattat
     1921 aagaatattc tggcttaatc agaaccggat cacggcaatt ccggaaagca tacttggcct
     1981 cagttctgat agcgttgtca atcttagaga aaatcaacta tctcccagaa taatgcaaac
     2041 tttgtt
//
LOCUS       DADLDT020000277         2041 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000277 DADLDT020000000
VERSION     DADLDT020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.283"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(219..383)
                     /locus_tag="JQH22_004316"
     CDS             complement(219..383)
                     /locus_tag="JQH22_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208395.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="JQH22_004317"
     CDS             1285..1569
                     /locus_tag="JQH22_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFB2208396.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..>2041
                     /locus_tag="JQH22_004318"
     CDS             1557..>2041
                     /locus_tag="JQH22_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2208397.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          528 a          428 c          583 g          502 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggctccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagca
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggagct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 a
//
LOCUS       DADLDT020000278         2035 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000278 DADLDT020000000
VERSION     DADLDT020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.284"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            307..576
                     /locus_tag="JQH22_004319"
                     /pseudo
     CDS             307..576
                     /locus_tag="JQH22_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            573..799
                     /locus_tag="JQH22_004320"
                     /pseudo
     CDS             573..799
                     /locus_tag="JQH22_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            828..1418
                     /gene="ospG"
                     /locus_tag="JQH22_004321"
     CDS             828..1418
                     /gene="ospG"
                     /locus_tag="JQH22_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFB2208398.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1757..2035
                     /locus_tag="JQH22_004322"
                     /pseudo
     CDS             1757..2035
                     /locus_tag="JQH22_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          573 a          446 c          486 g          530 t
ORIGIN      
        1 gtgatgcgtg agactgaaca gcagatttac cgtcagctga ctgacgaggt actggccctg
       61 cgattgtctg aaaacggctc acaactgcac cattcataat cacatcgcat aaaactgtca
      121 gcgccagtga taggacaggt gaaacctgtc aatacgtacc ggcttacgcc gggcttcggc
      181 ggttttactc ctctgccata tgcaacaacg gagtgccgcc ttacatgccg ctggcgcggc
      241 atatcctggt gacgatatct gaacagttat atactgtgta tatacgtggt aatgacgagg
      301 tggcaaatgg cacaggttaa tatgagtgta agaatcgacg cagaactgaa ggatgctttt
      361 atggccgctg caaaaagcat ggaccgtaat ggctctcagt taatccggga ttttatgcgc
      421 cagaccgttg aacggcagca caattcttgg ttccgtgact aggttgcggc aggccgtcag
      481 caactcgaat gcggtgatgt gcttccccat gacatggtcg aatcctctgc tgctgcatgg
      541 cgggatgaaa tgagcaggaa gattgctgat aaatgatgga gatgttctgg accatgctgg
      601 ccatccagga caggaagcgc attcgtagta cattgcggag cagaacctga tggccgccat
      661 tgaaccggac gaacggattg gttattcagc cagcagcctg gcagggcagc cgtataaagg
      721 ccgcaacggt cgtgtggaag gaacatctgg accacacaag gtagcgtgta acgttatttt
      781 gtgtgaaaat ctcttgtgat ttttaaatat cgtaacaaag gtatgacatg aaaataacat
      841 ctaccattat tcaaacacct tttccatttg agaataataa ttctcatgct ggcatagtaa
      901 cggagcccat tctcggtaag ttaataggtc aggggtcgac agcagaaatc tttgaagatg
      961 tgaatgattc atctgctttg tataaaaagt atgatcttat tggcaaccag tacaatgaga
     1021 ttctggaaat ggcttggcaa gaatctgagc tttttaatgc tttttatggc gatgaagcat
     1081 ccgttgttat acagtatggc ggagatgtgt acctccgaat gctgcgcgtg cctgggactc
     1141 cccttagtga cattgataca gctgatatcc ctgataatat agagagcctt tatctacagt
     1201 tgatatgtaa attgaatgag ttgagtataa tccattacga tcttaataca ggtaatatgc
     1261 tgtatgataa agaaagtgag agtttattcc caatagattt tcgcaatatt tatgctgaat
     1321 attacgctgc aaccaaaaaa gataaagaga ttatcgaccg acgattacaa atgcgtacaa
     1381 atgattttta ttcgttatta aacaggaaat atttatagac gtatttgttg atgctataag
     1441 ccagaggctc ttctatgaga atatggggac cattccctcg cgatattaac gtaatatctt
     1501 aattgtatgc accgccagaa tgcgttcagt gcgatcacgg cagcagacag gtaaaaatag
     1561 caacaaacca cccgaaaaac cgccgcgatc gcgtatgata aattttaacc acatgcatac
     1621 ctgtgcagca aggcgaatca cgcacggatt tccagccaga gacacctgaa acgtaaccgg
     1681 ctcatttaaa ccgtctggtc tgttacctcc ggttccacaa aaataatgtc catcattttg
     1741 aagtggacac tatcgtatgg aataccggac ctgaatcact gaagctttac gccttcattt
     1801 cgaagaacat ttaccacggg gtgtggccgg atgtcgcctg ggcgtaccaa aatcaactgt
     1861 ctgtggtatg ttcgtgcgct tccgcaacgc tggcctttca tggcctctgc ccgcaggtat
     1921 gtcggagcag gaacttgatg cccttcttta cggaagtgcc tccacagtac ctgtcgtact
     1981 tactgaaagc accgttatgc ccaaactccc ggtggtaaaa aaacgtcccc ggcgg
//
LOCUS       DADLDT020000279         2033 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000279 DADLDT020000000
VERSION     DADLDT020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.285"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..363)
                     /locus_tag="JQH22_004323"
     CDS             complement(<1..363)
                     /locus_tag="JQH22_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2208399.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="JQH22_004324"
     CDS             complement(430..1644)
                     /locus_tag="JQH22_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HFB2208400.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DADLDT020000280         2031 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000280 DADLDT020000000
VERSION     DADLDT020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.286"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>2031
                     /gene="sat"
                     /locus_tag="JQH22_004325"
     CDS             <1..>2031
                     /gene="sat"
                     /locus_tag="JQH22_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HFB2208401.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          745 a          358 c          401 g          527 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca g
//
LOCUS       DADLDT020000281         2008 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000281 DADLDT020000000
VERSION     DADLDT020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.287"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..537
                     /gene="traX"
                     /locus_tag="JQH22_004326"
     CDS             <1..537
                     /gene="traX"
                     /locus_tag="JQH22_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFB2208402.1"
                     /translation="FALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWYEG
                     NILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLMLAVSNRLYR
                     AEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLCAGKSLPRFWPGD
                     FFPTFYACHLAVLGVLAL"
     gene            592..1152
                     /locus_tag="JQH22_004327"
     CDS             592..1152
                     /locus_tag="JQH22_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFB2208403.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            1283..1495
                     /locus_tag="JQH22_004328"
     CDS             1283..1495
                     /locus_tag="JQH22_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFB2208404.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            1739..1978
                     /locus_tag="JQH22_004329"
                     /pseudo
     CDS             1739..1978
                     /locus_tag="JQH22_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
BASE COUNT          487 a          455 c          619 g          447 t
ORIGIN      
        1 tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca accggccatt
       61 aaccggctgt ggggatgggg aattattgcc cagttcgcat attatctggc aggctttcct
      121 tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt
      181 gaaacgcgca gtgggtggcg tacagcagcg gccatactcc tgatggccct gtgggggcct
      241 ttgtccggca ccagttacgg tattgccggg ttgctgatgc tggcagtcag caaccggctg
      301 taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgcctgct ggctgttatt
      361 ccggcgctta accttgccac cagtgatgca gcggcggtgg ccggtcttgt gatgacggtg
      421 ctgaccgttg gtctggtgtt gtgtgccgga aaatcattac cccgtttctg gcccggagac
      481 tttttcccga cgttctatgc atgtcatctg gctgtgctgg gcgttctggc gctgtgacgg
      541 gtgtggtatc tttggccgca agaggatgat tcgtcagagg cagaacacag catgacagag
      601 cagaagcgac cggtactgac actgaagcgg aagacagagg gaacagcgcc tgtccgcagc
      661 cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa gcagaagctg
      721 gccgagaaag cggcccgtga agcagagctg gcggcaaaaa aagcgcaggc cagacaggcg
      781 ctgtccattt atctgaacct gcccacgctg gatgaggctg tgaataccct gaagccctgg
      841 tggccgggat tatttgacgg tgacacgccc cggcttctgg cctgcggtat ccgggacgtg
      901 ttactggaag acgtggcgca gcggaatatc ccgctctcgc ataaaaaact gcgcagggcg
      961 ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg tgcctgccgg
     1021 tatgacacgg aagggtatgt gacggagcat atttctcagg aggaggaagc gtatgcggga
     1081 gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg cagaacttca ggccgtgctt
     1141 gatgagaaat aaaaaaagcc tcccccagag agaggaggca gggaaataag gcttaaaagg
     1201 aaataacttc cgcttccgaa aggaagccag tccactgaat tatttatcac aataatactg
     1261 acaactaaag ggggacaaga aaatgaatgg attcagaaac agttcacgga acggccaggt
     1321 ctggcgttac cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg gacgctggat
     1381 ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg cctaggacag actggcagca
     1441 gtttgcccgg aaaagggctg aacactgcca ccggcgctgc cggggaaggg tgtaacgaaa
     1501 aaagcagcct tcattactct aggagtcaga aggctgaaag gagtttatga gatttgcttc
     1561 ttaatatccg gtcgggcagt atgaccggac agttctgagt agcacagagt cttttccgca
     1621 ttaagtcgat tttttatatt ttgtgattag tttcacaaaa taaggtgtta ttcaatgtgt
     1681 gctgcactat tcaggggggc tgaaatacag gtggcattta tctgatatgg agaataacat
     1741 gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt ctgcagatat
     1801 tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg actcactgaa
     1861 ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg attatggccg
     1921 gtggtcaaca gacatgatga aatcactggt cgcaggaaaa acggttaccg tcacatattg
     1981 aggtgtactg gcaatagcgg acactacc
//
LOCUS       DADLDT020000282         1986 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000282 DADLDT020000000
VERSION     DADLDT020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.288"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            441..536
                     /locus_tag="JQH22_004330"
                     /pseudo
     CDS             441..536
                     /locus_tag="JQH22_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            534..664
                     /locus_tag="JQH22_004331"
                     /pseudo
     CDS             534..664
                     /locus_tag="JQH22_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            773..1039
                     /gene="ospE1"
                     /locus_tag="JQH22_004332"
     CDS             773..1039
                     /gene="ospE1"
                     /locus_tag="JQH22_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFB2208405.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1430..>1986
                     /locus_tag="JQH22_004333"
     CDS             1430..>1986
                     /locus_tag="JQH22_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFB2208406.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNQ
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          666 a          417 c          337 g          566 t
ORIGIN      
        1 tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca gatgactgct
       61 tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaagct tcttaccata
      121 tttgaaataa agctcaatag cgcgaatttt ttgttcttca gtaaacatgt ttcatcttct
      181 gagagtccag gaaatcgtcc gcatccccat taccgtctta tatcactggc gctgacatgt
      241 gctcattgat gaagtgaatc tgaatgacat gttttgccat atcaacgcca accggtgtat
      301 atttcatttg tggagtctcc agtctgagga cgctttatgc atcccgtatt gggcagtatg
      361 atgccggaaa gctgcgaggc tccacacctg ccagtttcac ccttttaacc atcatactac
      421 tccgtgttta attgtgtggg atgcgttcat tacattttaa cagtgtcacc ccgtcggcca
      481 cgaaagataa acaggtgacc agaaaaggga tcttcatcca gcacatgcta tatcttgaat
      541 gtaacaacct gaccctccgc acccgcatca agcggctggc acataaaaca atctgcttct
      601 cgcgttccgt ggaaatccac gaaaagtcat cggctccttc attgaaaaac acatattcta
      661 ctgattggaa gcgccacccg aaaaagtatc gtttttttac agtaaacttc atttagccga
      721 ctataatgta aaaaaataaa ttttttgcca aaatataaca taaggaagta atatgcttac
      781 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      841 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      901 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      961 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
     1021 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaat
     1081 atatcagggt ttgttactaa taacttctga aaacagaaaa aaaaatagag cagtaattct
     1141 atttcagaga ggagtaactc catctatcct cgatacgtac tgaacgttat cgtgatatat
     1201 tcatcatgct ttcttcacca ttattcgagt atagggagag acgcaatgac cttaaatacc
     1261 tttttatggc tgaattacca ttctcaggaa taacagcaga ctccttccca tagactgctt
     1321 agggggaaag caatacccgg agaaagggta caaagcaaac tgttttaaaa ttaataagct
     1381 atattctatt taactagaat tcttatcgtt gataagaatt ctagttataa tgattaaatc
     1441 aaccaatata caggcaatcg gttctggcat tatgcatcaa ataaacaatg tatactcgtt
     1501 aactccatta tctttaccta tggaactgac tccatcttgt aatgaatttt atttaaaaac
     1561 ctggagcgaa tgggaaaaga acggtacccc aggcgagcaa cgcaatatcg ccttcaatag
     1621 gctgaaaata tgtttacaaa atcaagaggc agaattaaat ttatctgagc tagatttaaa
     1681 aacattacca gatttaccgc ctcagataac aacactggaa ataagaaaaa accaattaac
     1741 acatctccct gatttaccac caatgcttaa ggtaatacat gctcaattta atcaactgga
     1801 aagcttacct gccttacccg agacgttaga agagcttaat gcgggtgata acaagataaa
     1861 agaattacca tttcttcctg aaaatctaac tcatttacgg gttcataata accgattgca
     1921 tattctgcca ctattgccac cggaactaaa attactggta gtttctggaa acagattaga
     1981 cagcat
//
LOCUS       DADLDT020000283         1985 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000283 DADLDT020000000
VERSION     DADLDT020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.289"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="JQH22_004334"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="JQH22_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFB2208407.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          624 a          340 c          318 g          703 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tactaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg acatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcaaaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaaagaggg taatgctgcc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     1981 tgcgt
//
LOCUS       DADLDT020000284         1974 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000284 DADLDT020000000
VERSION     DADLDT020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.290"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..836)
                     /locus_tag="JQH22_004335"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="JQH22_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1313..1549
                     /locus_tag="JQH22_004336"
     CDS             1313..1549
                     /locus_tag="JQH22_004336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208408.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          533 a          464 c          456 g          521 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attggacgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1501 ggagatagaa aagtttaagt tccaaagtga agactgcgaa cctcgctaat tgattactgg
     1561 aacgattgtg tatgccagac agtgcaatac tagctctggc aagattacca tgtatttttg
     1621 tccagaatgt tttgcgaaga gagcaatatc tatattgcaa cctttcccta tcgaggaagg
     1681 cgataaatat acgaaaactc aatgcccctc ctgtaaaaat gagtatctca tggactacaa
     1741 tttcaataat gatttttgag tcaaggtatt cagattttta aagaacatgc cggatactca
     1801 cccgtgtccg gcgcacgcac tccacctcac ccgtggagaa ctccttaatt accaaccttg
     1861 gcttcgttgg ttagccatta acgctggtat gtaatcattc tggcaatgct taatttgaag
     1921 tggcacactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaa
//
LOCUS       DADLDT020000285         1936 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000285 DADLDT020000000
VERSION     DADLDT020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.291"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..546)
                     /locus_tag="JQH22_004337"
     CDS             complement(<1..546)
                     /locus_tag="JQH22_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208409.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1201..>1936)
                     /locus_tag="JQH22_004338"
     CDS             complement(1201..>1936)
                     /locus_tag="JQH22_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208410.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          545 a          441 c          417 g          533 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggaacctag gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa
      841 ggatgatgaa tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc
      901 ccgaagtcct aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg
      961 ggaagtcgct gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc
     1021 caaggtcata acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca
     1081 atagcgagta tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg
     1141 accatataca actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag
     1201 ttactctgcg gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac
     1261 gtcaaactca cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat
     1321 taactcaagg tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt
     1381 agcgtcttta gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc
     1441 tgtatcatcg cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg
     1501 acctgcgcgt tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc
     1561 gtaatcgctc agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag
     1621 atcttcagct tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc
     1681 tgacgtatct ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg
     1741 catatcgcat acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc
     1801 agacacatga gtagcagcag aacacaggat gcccgtctta ttggtctcag ggttcaacac
     1861 tggggtgaca gttacgcttg cccctttgat gactgtggta gacggagcat ccttagctgg
     1921 attaaacgcg ccatta
//
LOCUS       DADLDT020000286         1936 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000286 DADLDT020000000
VERSION     DADLDT020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.292"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQH22_004339"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQH22_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HFB2208411.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1936
                     /locus_tag="JQH22_004340"
     CDS             1494..>1936
                     /locus_tag="JQH22_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFB2208412.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          552 a          337 c          466 g          581 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatga
//
LOCUS       DADLDT020000287         1913 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000287 DADLDT020000000
VERSION     DADLDT020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.293"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(53..1702)
                     /locus_tag="JQH22_004341"
     CDS             complement(53..1702)
                     /locus_tag="JQH22_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFB2208413.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DADLDT020000288         1885 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000288 DADLDT020000000
VERSION     DADLDT020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.294"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            214..518
                     /locus_tag="JQH22_004342"
                     /pseudo
     CDS             214..518
                     /locus_tag="JQH22_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            687..>1885
                     /locus_tag="JQH22_004343"
     CDS             687..>1885
                     /locus_tag="JQH22_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2208414.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSRPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGT"
BASE COUNT          487 a          376 c          428 g          594 t
ORIGIN      
        1 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
       61 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      121 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      181 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      241 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      301 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      361 aggactggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      421 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      481 caagaataaa tagtagtaac tactatagtt gcaaataatg ttaaaaacat ttctatgatc
      541 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      601 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      661 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      721 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      781 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      841 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      901 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
      961 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1021 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1081 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1141 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1201 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1261 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1321 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacgcc
     1381 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1441 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1501 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1561 aggatatggg aaaactggca tcaatggggg gggtttctgt ttttgaaatc ggtggtgtgg
     1621 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1681 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1741 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1801 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1861 gagccgcatc agggctgtca ggcac
//
LOCUS       DADLDT020000289         1857 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000289 DADLDT020000000
VERSION     DADLDT020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.295"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(93..1118)
                     /locus_tag="JQH22_004344"
                     /pseudo
     CDS             complement(93..1118)
                     /locus_tag="JQH22_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1176..1857
                     /locus_tag="JQH22_004345"
                     /pseudo
     CDS             1176..1857
                     /locus_tag="JQH22_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          464 a          445 c          472 g          476 t
ORIGIN      
        1 gctaacctga tggatcgcca tgcggatttc attaatcgct ttgatgcgat catcttccgg
       61 caagctggcg agaaattgcg ttaaatcctg cgttaatcgt tgttgcatag tatcccccat
      121 tcctgacgtt ttccttttat ccgttcgtta taacgctgat agttcttcgc tttcgtagga
      181 ctaaatgata atgcccgaca ccagtaatcg ttattgagta aaactttgca tatccgccgc
      241 caggagggga tatctttcgc gccaatgtcc ccttgctggg tttgtggaac ctcgatgcct
      301 tttttctgat accagtgcaa ataaatggcg attttattgc ggtaatgctc agctgttttt
      361 tccggcatgc tattgagcag caataatgca tattcttgct agtctaaatg ttctggtttg
      421 aggattttcc gatgcccata gaaatgattg tcatgtccgg cgtaaatgcc gccacttttt
      481 accccgctga ctcgtgcgca catagcagcc cagcgatccg gttcgataac gtgacagagc
      541 cataatcctt gtcgttgctc cgggccaaaa ggttcgcaaa ttcgcatatg acgcagagga
      601 acgcctgcct gatacattag gttatacagt gggttgcaga gattttgatg atttgcatat
      661 caggtccaga tatcagccac tttccagtcg taaatggggt aaatgtacca gctatgaccg
      721 cctggtgcgg ccgtagtcca tggtttatcg tcggcaaaac gttgtttatt taaactggcg
      781 atggcgacaa aacggttgta ggactcatcc gcacggatgc cgatcatcat cgccgccgga
      841 cgtttttgtg aaaaccattc ggcaaactca cgtacaaatt gttcgaaggt catgcctggc
      901 tggtaaaagc agaaaaagtc aggagcggtt atcgcatctt gcgggggctg acgcacccat
      961 tcgacatcag gttcccagca ctgccattcg ggttggtatt gtgaaaggga attttgcgtc
     1021 gtaagcggga gcgcaaccca gtaaaactct tcgatgacat cggtgtacaa ctcgcgcagg
     1081 gactgaacat agttaatggt gcaagagaat tgtgaatcgc cacgggttta acagacacct
     1141 cagagtcatt taagatggct taaagagagg tgcccatgag cggtaagcgt tatcccgaag
     1201 agtttaaaac tgaagcagtc aaacaggttg ttgatcgcgg ttattctgtt gccagcgttg
     1261 caacacgtct cgatatcacc acccacagcc tttatgcctg gataaagaag tacggtccgg
     1321 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
     1381 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
     1441 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
     1501 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
     1561 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
     1621 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
     1681 gtgcggagta aacagagtct ggagactgat gaaacgtgtc ggaataaagg ctcaggtcgg
     1741 ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc gtgtcaccca acaggctcca
     1801 gcgacagttc aatccggatg ctcctgatga gcgttgggta acggacataa cctacat
//
LOCUS       DADLDT020000290         1821 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000290 DADLDT020000000
VERSION     DADLDT020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.296"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..185)
                     /locus_tag="JQH22_004346"
     CDS             complement(<1..185)
                     /locus_tag="JQH22_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208415.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..1213
                     /locus_tag="JQH22_004347"
     CDS             371..1213
                     /locus_tag="JQH22_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFB2208416.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1216..>1821
                     /gene="capU"
                     /locus_tag="JQH22_004348"
     CDS             1216..>1821
                     /gene="capU"
                     /locus_tag="JQH22_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFB2208417.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          528 a          338 c          397 g          558 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga aaagacgact
     1141 tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg ggccaagatt
     1201 accggatgac taataatgaa tatactattt acggaatcat caccgaatat aggtggtcag
     1261 gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc agttctgctt
     1321 gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat tgatatcaca
     1381 ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact cggaatagtt
     1441 catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa tattgttggt
     1501 ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa gacatatttg
     1561 acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat tgttcccgga
     1621 acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct
     1681 cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc tccgagtgtt
     1741 ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc
     1801 ccggaaaaag ggcacgaatt t
//
LOCUS       DADLDT020000291         1810 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000291 DADLDT020000000
VERSION     DADLDT020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.297"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..319)
                     /locus_tag="JQH22_004349"
                     /pseudo
     CDS             complement(1..319)
                     /locus_tag="JQH22_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(319..1239)
                     /locus_tag="JQH22_004350"
     CDS             complement(319..1239)
                     /locus_tag="JQH22_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFB2208418.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1377..>1810
                     /gene="pgrR"
                     /locus_tag="JQH22_004351"
     CDS             1377..>1810
                     /gene="pgrR"
                     /locus_tag="JQH22_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFB2208419.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          452 a          434 c          420 g          504 t
ORIGIN      
        1 cagcatcaac atatggcaga gttactgcat aatcgataac acggctaata tcctctacgc
       61 gctgtgtcgg atcttctatc caacgaggct acccaccaga ttctccctgg aaactggcgt
      121 cataagcgag aacgacatag cctttttcag ccaatgcctt gccatagata ttgccagacg
      181 tctgctcctt acaactgcca aaaggatgga cgctgataat cgccggatac tgttttgact
      241 catcaaaatt tggtggaaac tgaatattgg cagcaatatg ccaggccata tcactattgc
      301 ggatttgaat atgtttcatt acagagtttc ctcaaagaac ggagctaata ctgaaattgc
      361 ttcagccaca taggctttgc cgtcataaag atccatatgg tttgcgcctt caacaatgtg
      421 atagcgttta tcctggcttg atgcccgatc gtacaggtcg tcactcatcc atttgctccc
      481 tgcttggctg cccgccacaa tctgcatcgg ctgagtcagg tacacttccg ccatatggta
      541 agcatcatag gtaataatct ggttaaggct gcgcaaagta gcgtaacccg gtgctgttgg
      601 atactgcgca cgaggtgtgt ggtaatactc ccaggcctga cgcagttctt cgttcggcgc
      661 atcggcctcc ttcattggag ccagtggcat aatggcgtat tcttcgctgc taatatcgct
      721 ggttcgggcg tttgagccag cttcaatgtg tggcagagca tcaatagatt tcacattgtt
      781 ttcccaacca ttgcggaaca tcgaaccaat attgaccgca ctaacggtac cgatggcctt
      841 gatgcggcga tcctgaattg cagcattggc tgtatatcct gcaccggcac aaattcccat
      901 cgcaccaatt cgggtattgt cgacataaga aagcgttgtc aggtaatcaa tcacggcact
      961 gacgtcttca gtacgaatgt atgggttttc taactgacgc ggctcgccgc cactttcacc
     1021 ttgataagat gcgtcataag caatagtgac aaatcccttt tccgccagct tttcggcata
     1081 ggttccggcc gtttgctctt taacgccccc acctgggtga gataccacaa ttgccggata
     1141 ctgacgggtt tcatcaaatt ttggagggaa atagatcact gcagacaaag agatggttgg
     1201 attattgctg ttagtgaagc tgactttatt attcatcatt cagttcctca tggagttgtc
     1261 ggttcgtttt gacggttggt gatatcacta tagatattga tcattaagtt gattagaccc
     1321 taaatcatga ttagacctat catttaaatg attaatagat tgtatggaga tactgaatga
     1381 agcgtgaaga aatcgctgat ctgatggcgt ttgtcgtcgt tgcagaggag cgtagcttca
     1441 ctcgcgcagc agcccgcctg agcatggtgc agtcagcttt aagccagata gtgcgccgta
     1501 tagaagaacg attgggattg cggcttctga cgcgaaccac gcgcagcgtt gtacccactg
     1561 aagcgggcga gcatcttttg tctgttcttg gccctatatt gcatgacata gattctgccc
     1621 tgacatccct gagcgatctg cagaaccgcc catccgggac aatacgtatt actactgtag
     1681 aacatgcagc aaaaacgata ttgttaccag ccatgcgcac attcctgaaa tcgcatcctg
     1741 aaattgatat tcagcttacc attgattatg gtttgaccga tgtcgtttct gaacgttttg
     1801 atgcaggcgt
//
LOCUS       DADLDT020000292         1810 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000292 DADLDT020000000
VERSION     DADLDT020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.298"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..570
                     /locus_tag="JQH22_004352"
     CDS             <1..570
                     /locus_tag="JQH22_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFB2208420.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="JQH22_004353"
     CDS             571..936
                     /locus_tag="JQH22_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFB2208421.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTYAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="JQH22_004354"
     CDS             933..1616
                     /locus_tag="JQH22_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HFB2208422.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          504 a          383 c          500 g          423 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgtatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc
//
LOCUS       DADLDT020000293         1808 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000293 DADLDT020000000
VERSION     DADLDT020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.299"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            173..1198
                     /locus_tag="JQH22_004355"
     CDS             173..1198
                     /locus_tag="JQH22_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFB2208423.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1808
                     /locus_tag="JQH22_004356"
                     /pseudo
     CDS             1266..1808
                     /locus_tag="JQH22_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgtt
//
LOCUS       DADLDT020000294         1780 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000294 DADLDT020000000
VERSION     DADLDT020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.300"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(114..401)
                     /locus_tag="JQH22_004357"
     CDS             complement(114..401)
                     /locus_tag="JQH22_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208424.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(651..806)
                     /gene="hokD"
                     /locus_tag="JQH22_004358"
     CDS             complement(651..806)
                     /gene="hokD"
                     /locus_tag="JQH22_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFB2208425.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1034..1699)
                     /locus_tag="JQH22_004359"
     CDS             complement(1034..1699)
                     /locus_tag="JQH22_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFB2208426.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          463 a          454 c          397 g          466 t
ORIGIN      
        1 aaaatcgcaa ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag
       61 ttgtatatgt gccatcggct ttctccggtg gcacggtgtt acacagcagg agttcaatcc
      121 tgctcaagat tgtagatgag tttattcttc agcaaaagca gaaaaccagc cttaaaacca
      181 atctttttca aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc
      241 gtcagaagtc cattacaaaa ataaaataac acagcaccgc tcttcctttg ttgagattgc
      301 aaacatctaa tgcggcaatg gctgacaatc gctccattct caacgcgcac agcatagagg
      361 ccattttcac taaaaatttc acgcaattct tcgattttca tcttcagaat ccttccagat
      421 aaatagctct cccctgttcg gggtccatcc ctcttctccc tgcgcgctac ttaagtgagt
      481 cgattctatc tgcgaaggtg cgcgaatcaa attcaccgga aataaacaac aaaaaacccg
      541 ccgaaacgct gagagatcac agcgatgggc gggttaagtg cgggtgcgtt gaggatgcct
      601 gacacatcag aggtgggcgg ggatgggatc agctccccac ccggtcactc ttacttcctg
      661 gattcgtagt ctacgaagac agcgacctcc gtctggccgg ttcggattcg tacctcacag
      721 aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg
      781 attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg catgtaagag
      841 gctaacctac atgtgcaaag catgaaattg gcctcagatt aatgttaagc gtcttgccgg
      901 acgcgtaatg ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgaagcag
      961 atagcctcaa gcacccgcag tcattctact tacctcgcca atatgaaatc aatcagaaag
     1021 gtgccccata aaatcactcc ttctcttctt taccgtagtg gagttgacca attttgataa
     1081 gagggcgtcc ctgagatttg cggtgtagat tggtatcgcg cagagaatac acacagccac
     1141 aatattcctg ctgatagaat ttttcgcgct tgctgatttc aatcatacgg gacgagccgc
     1201 cctgcttgcg ccagttataa tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc
     1261 gcccacagtc gttaacctgc tgcatatttt tccagcgtga aatgcccagt gaactgctga
     1321 tcacactgaa accattttca gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac
     1381 acatggtgca acggatccct cgttcgggct cccattccat tcctttggct cgttcaaacc
     1441 agttgtctgt gtcgtaatca gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa
     1501 tattctcatc cttacgaatt aaatactctt tctgaggatg aatgttcggg ttgtagaaaa
     1561 agatggtgta gtcgattccc gaggcctgaa gcgcctccat cacctcaccg gaacatggag
     1621 cacagcaaga gtgcagtagt agtttgtttg ccccgtttgg gagctccaat ttgggccgtt
     1681 tgaaatcagc aactgtcata aatatgttta ttgaggtcat aaaaatatca aagagtgtag
     1741 cattagaacg gggctatcgg aaacagatgt gtgactgctc
//
LOCUS       DADLDT020000295         1770 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000295 DADLDT020000000
VERSION     DADLDT020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.301"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(163..630)
                     /locus_tag="JQH22_004360"
     CDS             complement(163..630)
                     /locus_tag="JQH22_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFB2208427.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(696..935)
                     /locus_tag="JQH22_004361"
     CDS             complement(696..935)
                     /locus_tag="JQH22_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFB2208428.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1070..1675)
                     /locus_tag="JQH22_004362"
     CDS             complement(1070..1675)
                     /locus_tag="JQH22_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFB2208429.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          563 a          392 c          318 g          497 t
ORIGIN      
        1 ggcacagttg gtgtggcggc ctcagtccgg aacaatttga aaaccagaac cttgcttagg
       61 cctgtgtcca tattacgtgg gtaggatcac aagacctact gctatcacaa ttactgagag
      121 aaaaaaaagc atccgatgat tcatttatgc tgtcctaaaa aattaacaaa acaatgggaa
      181 aacaggatat gtaagcagaa aagccaatga aattggtaca gcataaggca caccgttttc
      241 acgaattgat tttcgaaaaa aaagaagacc tgccagaaca accagagcgc cgcaaaaggt
      301 cattgcgacg agaaaattag ccagttcatg ttcatcaata gctaacgaaa gaacagttaa
      361 cagcttgaca tcaccgccgc caattatccc tgcggaaaaa agaaagaagc ccacgagcaa
      421 aacgctcccg gctgcaacca gattggtttt ttgatgctgt aaaatgagtg ccaaaaaaat
      481 aagcacggtt aacaccagta aataacgatg gctaataatt ctgagtaata tatcccccca
      541 tgaaataaaa ataagaaaga gcgcaacaaa taaaagatcg actcttagta accatattgc
      601 tgccattgaa agactccatg taccatacaa ggttgctgcc agacatattc ctttacgtct
      661 gacagcctct agagagtttt tttatttttt ataagttacg gattactcgt tccggaaatc
      721 gttacctgtt gaattgctgc agcgatagcg tcgaatgcat ctttaattgc gcccaggaag
      781 cctgagttct gatcgccaaa gatgaaagca agcagagttg ccatagcaac accaatcaat
      841 gcatattcga ttgcagtaac accacgttca tccttcgcga aacgcgcaaa gcactgttta
      901 gttttaccat agtacttagt aataaaagta ttcatgtttt cctcgtatat tattatttaa
      961 attcaaacca ccagcttcat ctgcgtgtca ccggtatccc atattttttg gatgtggcta
     1021 taattatcca ttcccgctat ttagtcttca aaaaaaatta catttaatat catttatctt
     1081 gattaagaaa tagtaagact aaaaaaacaa gcgtcgtcag ccagcacact gctaacacgg
     1141 taagaacgct aataaaaaca ctccactgct tcgttctata ccattcaata tttttgtcag
     1201 tatgttttat tgggagcagc tcttcagtag agaattcctg gctgttatca ttctcaggaa
     1261 tgtttgcatc agtgtttact tccctggcct cgctatcgtt ggtatagcta ctactgtctt
     1321 catcactgtc acgctcattc tcacagacat gtttctcgac ctttcccaga tatctgacgc
     1381 cttttcgcgg aatggtttta attagagaac tcgacaaacc tgcgacgtct aatgcctttc
     1441 taagcatgta aatctgccca taataactgc tgtcgcttac cgaacctttc tgctcccgcc
     1501 aaacctcatt gataatatgt tgcttatcgg attctccctg tagtaaaagg agtaaaaatc
     1561 gatagccgtt ttcagacaga ttgattacat tgcctgtact ggtggtgata cgtcgagacg
     1621 ctgggtaaaa cttaactccc tgccctaaag aaaaataaaa acggtcatcg ctcataagta
     1681 ccgacccctg ttttcttctt cacatgacta ctcattacaa ctttgtatga agtaagatgc
     1741 aaaatcatat tgtttacatt aagttaagca
//
LOCUS       DADLDT020000296         1759 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000296 DADLDT020000000
VERSION     DADLDT020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.302"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(1..263)
                     /locus_tag="JQH22_004363"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JQH22_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="JQH22_004364"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="JQH22_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFB2208430.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="JQH22_004365"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="JQH22_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          501 a          435 c          318 g          505 t
ORIGIN      
        1 agcaagcact gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatctt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DADLDT020000297         1745 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000297 DADLDT020000000
VERSION     DADLDT020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.303"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            452..1300
                     /locus_tag="JQH22_004366"
     CDS             452..1300
                     /locus_tag="JQH22_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFB2208431.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1317..1598
                     /locus_tag="JQH22_004367"
     CDS             1317..1598
                     /locus_tag="JQH22_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208432.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          497 a          399 c          410 g          439 t
ORIGIN      
        1 cattatatag ttctactctg gcgagatacg ttggcatatt catttcctta ctggttgtgt
       61 gagaacttca gtaaagatac caccaaagcc cggaagtggt gaaataaaac cgggcacaac
      121 acgaaggcgc atttccggta tccataaaga gtcggtcttg tctgtgaaat ttaaatggtg
      181 ggagtgcgct tccggttgta aataacgaca ttgctgtgtg aagtaccagt tggcggcatc
      241 ggtttaattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      301 ggctatgcgc acgcggcaca gttaaaagtc atgttagtcc ttattggttt gggtgggaaa
      361 gccaactgta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcaacaa
      421 agttcacttc ggtgatgaaa ggaaagagaa aatgttgaat gtagctattg aaaaccagaa
      481 cgggtggaat tatagtgcac ctgcacctca taaaacgggt gccgggattg ctaccccgac
      541 gatgactact gcgcataacc gcgctcaggc ggttttttta tgcgtcaagc acagccacat
      601 tcagattatg gtggggcgtg cagggcagcc gcaaggctgg ccggtttcgg tagtcaccgg
      661 ttgtagcaac cctgtgcgtc tcaccaccca tgagattgct acctctggtg gtgagtcatt
      721 taaactgact atcgaggctg ccatcatggc tactatcctt accctttctc accctgacgc
      781 aaccatcgaa aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa
      841 aatgcacaaa aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg
      901 gcttaatttt aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca
      961 aatcaccaaa aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc
     1021 attcaaggaa gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa
     1081 caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga
     1141 caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg tctgtaccct
     1201 ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc ggggcgcaat
     1261 taaaaatatg actgtggaac aattactttc tttaaaataa aaatggagct acggatatga
     1321 accctatgct tgattattgt tttacctctg cacctcagca gaccataaca cacaccggaa
     1381 tcaaaaattt gttatggata atcaaaaaaa acctccagca gaaaggtgat cccgatttaa
     1441 ttccctgtgc ttatggcctg tgtgacagac aagtaagccc tgataaggct tacactgctg
     1501 aacttattta catgcacgga accatgatga ggaaaaagaa acgcaaatat tgtagtgaaa
     1561 tctgcgccga aaaagaccag atggcacacg aactttaatt aactgactat tcgaaactga
     1621 atttatgcca gcaatggcag ggattcgctc aaccttaatt aaggagaaaa acatgaacac
     1681 caattatgaa gccactgttg caactactga taacattgtt cacgaggttt acctggaagg
     1741 aaagc
//
LOCUS       DADLDT020000298         1706 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000298 DADLDT020000000
VERSION     DADLDT020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.304"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..177
                     /locus_tag="JQH22_004368"
     CDS             <1..177
                     /locus_tag="JQH22_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208433.1"
                     /translation="ASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET
                     FSVSGTESSATRQH"
     gene            complement(310..822)
                     /locus_tag="JQH22_004369"
     CDS             complement(310..822)
                     /locus_tag="JQH22_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFB2208434.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1017..>1706
                     /locus_tag="JQH22_004370"
     CDS             1017..>1706
                     /locus_tag="JQH22_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208435.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGID"
BASE COUNT          502 a          381 c          432 g          391 t
ORIGIN      
        1 gcaagcttga taatggtggt cgggtgctcg tcaaaggaaa atgccctgtg ccatcctcag
       61 ccgaaacctc cagcgcctcc ggcatgggca atgatgccac cgtcgaactc tctccagttg
      121 ctggacgaaa cgttctcggt atccgggacg gaatcatcag cgaccagaca gcattgagaa
      181 tgcttcagga gtacatcagg atccaatgcc ttggggggta gcggtaattt tactcattat
      241 ccttcaaatc agattctgtt gtcagaggaa tgggggaagc tggattcgca agagcttttt
      301 atgggcagtt tagctaaaaa aaggcaacac gaagctaaaa ctaccaacat aaagtgctga
      361 ccagctagca gctgaaatca gactcactgt atacactttc actggatgaa gttgcaacat
      421 tcctgccacc aaaggagcaa tgtaacgcag cacggcgata aagcgcgaag tgaaaagtat
      481 tgacacagag ttattttgca actgaaggcg aacccgttct attgtattgg aacggctcga
      541 tattatacat gcaagttgag ggatatgccc aattattata ccaagatggt aattaacgat
      601 agtaccgcac catgcgccac tcatgaccgt gattccagct tcccatggtg aaagagtcgt
      661 cttgctgact gtaattacag tggccatcat aattgatgct ggtggcaata tcgccgaaat
      721 gagtaaagtg gattttgtga acgctattat aaaaagaagc ccccataggc gatatggatg
      781 aagtacaaaa tagttcatca aagcatttat ccactccatc aatgtctttc cttctgtgaa
      841 tttttctttg ctatttaacc gtgatacgac ttaatttagg cttaaacacc ttacatttac
      901 aaggtactcc tgtggggggc cctgccacgg agcgtcgcag gagtgagtga tgaggaagaa
      961 ttaaagcaac ctattgtgac aggcagcgat aacagtaaat cacaggaagc ataatcatgg
     1021 caaaaccgga ctgggaggcc atcgaatcgg cataccgggc cggagtcctt agcctccgtg
     1081 atataggcga gaaatacggc gttacagaag gggctatcag gaagagggcc aaaaagcttg
     1141 attgggcacg cagtggcggt acgcaggttt gcaaaaatgg tacgcaaaaa aggaaagtgc
     1201 gtaccagcag aaagcctgcc attactggcc ttacacaaaa aagtacgcaa ctaaaaacag
     1261 aatctacacc ggatacgaaa ccgatacgcg gaatgcgtac cgatcccccg actaacccat
     1321 tccaacccgg taaccagcag gcattaaaac acggtggtta tgcccgtcgc cttctgctca
     1381 aagatgaggt gatagaggac gctaaagcgt tgacgctcga ggacgaatta tttcgccttc
     1441 gtgctaacaa ccttgtcgct gcagagaata ttggtcggtg gttggtgtcg ctggaagatg
     1501 ctaatgggga ccaggaaagg aagatgctga ttgaaaatat cagcgccgcc gagaaagcaa
     1561 tgatgcgcaa tacagttcgt attgagtcca tcgttggcac gcttgcgacg gtaggaaaaa
     1621 tatttgctga tacagcctac cgcaaggccg ccactgataa ggtgtctctg gaggctgatc
     1681 gtcttcgccg tgatgcaggt attgat
//
LOCUS       DADLDT020000299         1703 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000299 DADLDT020000000
VERSION     DADLDT020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.305"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(854..1327)
                     /locus_tag="JQH22_004371"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="JQH22_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="JQH22_004372"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="JQH22_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          474 c          412 g          438 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aag
//
LOCUS       DADLDT020000300         1664 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000300 DADLDT020000000
VERSION     DADLDT020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.306"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            34..138
                     /locus_tag="JQH22_004373"
                     /pseudo
     CDS             34..138
                     /locus_tag="JQH22_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            381..1457
                     /locus_tag="JQH22_004374"
     CDS             381..1457
                     /locus_tag="JQH22_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HFB2208436.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTTRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLEVLEVTAPDRMERRKRGKSD
                     KIDAECAAHAAFSGIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQINSIPITA"
     gene            1477..1664
                     /locus_tag="JQH22_004375"
                     /pseudo
     CDS             1477..1664
                     /locus_tag="JQH22_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          427 a          399 c          439 g          399 t
ORIGIN      
        1 gccatgtcgt tctcgccgga cagcaggctc accatgaaat cgctggaaat ggcatgggaa
       61 acccgtggta agcccggcgg ggtgatgttc cacagcgata gtaataatgc cggtatcagt
      121 ttttatcatc actctgtttg ctgtttaacc agactggtgt gattactgat gcagtgaaga
      181 ccttcccgca tcctgactca cacagcgatc gaccctttgt gtcctgccct ggacctgtcg
      241 gttgccggaa gcgtcttcat gcgaggcgtc tcctcaccga tgcgcgtgac tcaagaaggg
      301 cctgacggct tgtctcgtta ctgtcctgtc cgggttatct gtctggagat tcaactctgt
      361 ttcctcacag gagctctgtt atggcaggta aagttacgga aaccgctgtt gtgggtggcg
      421 tggatacaca taaagatctg cacgttgccg ctgtcgtaga tcagaacaat aaagttctgg
      481 ggacccagtt tttctccaca acacggcaag gttaccggca gatgctggca tggatgactt
      541 cgtttggggc attaaagcga attggtgttg agtgtacagg cacctatgga tcaggtctgc
      601 ttcgctattt acagaatgcc gggttagaag ttcttgaggt gactgcgcca gatcggatgg
      661 agcgacgcaa acggggtaaa agtgacaaga ttgatgctga atgtgccgct cacgccgcat
      721 tctccggaat aagaaccgtc acacccaaaa cgcgcaatgg catgattgag tctctgcggg
      781 tattaaaaac ttgccgaaaa acagcaatat cagcccgcag agtcgctctc cagattatcc
      841 attccaatat tatctctgcc ccggatgaat tacgtgaaca actcagaaat atgacgcgca
      901 tgcagctcat caggactctg ggatcctggc ggcctgatgc cagtgaatac cgcaatgtta
      961 ccaacgttta tcgtatttca ttaaagtccc ttgcccgacg ctatctcgag ttacatgacg
     1021 aaatcgctga tttggatgtc atgattgcgg caattgtcga tgagctggcg cctgaactga
     1081 ttaaacgtaa tgctattgga tacgaaagcg cttcgcagtt gctgatcacg gcaggagaca
     1141 atccccaacg attaagatca gaatcaggtt ttgcggcact gtgtggtgtc agccctgttc
     1201 ccgtatcttc aggaaaaacg aatcgttatc gacttaaccg gggtggagat cgtgctgcaa
     1261 atagtgcact tcacatcatt gccatcggac gtttgcgaac tgacgataaa acgaaggaat
     1321 atgtcgccag acgagtagcg gaagggcata caaaaatgga agcaatacgc tgcctgaagc
     1381 gctatatctc acgcgaagtt tatacattac tgcgtaatca aaacaggcag atcaacagca
     1441 tcccgataac ggcttgactc ttagaagggc gtccatggca gtcattatac gagcaggcag
     1501 ttccggcagt tactgtggcg ataccagatc aggcagagta tgagccggcg cggaaactgc
     1561 tgggataaca gcccaatgga acgcttcttc agaagtctga agaacgaatg gataccggtg
     1621 gtgggttacg taagcttcag cgaggcagct cacgccataa cgga
//
LOCUS       DADLDT020000301         1655 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000301 DADLDT020000000
VERSION     DADLDT020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.307"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(156..1391)
                     /locus_tag="JQH22_004376"
     CDS             complement(156..1391)
                     /locus_tag="JQH22_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFB2208437.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JQH22_004377"
     tRNA            complement(1538..1614)
                     /locus_tag="JQH22_004377"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          450 c          394 g          391 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLDT020000302         1612 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000302 DADLDT020000000
VERSION     DADLDT020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.310"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            102..>1612
                     /locus_tag="JQH22_004378"
     rRNA            102..>1612
                     /locus_tag="JQH22_004378"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          447 a          338 c          503 g          324 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa gg
//
LOCUS       DADLDT020000303         1601 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000303 DADLDT020000000
VERSION     DADLDT020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.311"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(333..479)
                     /locus_tag="JQH22_004379"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="JQH22_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="JQH22_004380"
     CDS             533..715
                     /locus_tag="JQH22_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HFB2208438.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="JQH22_004381"
     CDS             complement(816..1145)
                     /locus_tag="JQH22_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFB2208439.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="JQH22_004382"
     CDS             complement(1317..>1601)
                     /locus_tag="JQH22_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208440.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          376 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt cagaaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DADLDT020000304         1597 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000304 DADLDT020000000
VERSION     DADLDT020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.312"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            154..477
                     /locus_tag="JQH22_004383"
     CDS             154..477
                     /locus_tag="JQH22_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFB2208441.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="JQH22_004384"
     CDS             474..890
                     /locus_tag="JQH22_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFB2208442.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="JQH22_004385"
     CDS             856..1380
                     /locus_tag="JQH22_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208443.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1597
                     /locus_tag="JQH22_004386"
     CDS             1377..>1597
                     /locus_tag="JQH22_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208444.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVL"
BASE COUNT          389 a          378 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcag
//
LOCUS       DADLDT020000305         1597 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000305 DADLDT020000000
VERSION     DADLDT020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.313"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..636)
                     /locus_tag="JQH22_004387"
     CDS             complement(<1..636)
                     /locus_tag="JQH22_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFB2208445.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="JQH22_004388"
     CDS             complement(750..1322)
                     /locus_tag="JQH22_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFB2208446.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DADLDT020000306         1538 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000306 DADLDT020000000
VERSION     DADLDT020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.314"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(910..1386)
                     /locus_tag="JQH22_004389"
     CDS             complement(910..1386)
                     /locus_tag="JQH22_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208447.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DADLDT020000307         1496 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000307 DADLDT020000000
VERSION     DADLDT020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.315"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1350
                     /locus_tag="JQH22_004390"
     rRNA            <1..1350
                     /locus_tag="JQH22_004390"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          382 a          341 c          456 g          317 t
ORIGIN      
        1 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
       61 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
      121 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
      181 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
      241 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
      301 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
      361 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
      421 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
      481 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
      541 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
      601 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
      661 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
      721 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
      781 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
      841 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
      901 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
      961 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     1021 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     1081 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     1141 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     1201 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     1261 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     1321 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     1381 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     1441 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaa
//
LOCUS       DADLDT020000308         1435 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000308 DADLDT020000000
VERSION     DADLDT020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.316"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..371
                     /locus_tag="JQH22_004391"
     CDS             <1..371
                     /locus_tag="JQH22_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208448.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="JQH22_004392"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="JQH22_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFB2208449.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..>1435
                     /locus_tag="JQH22_004393"
     CDS             738..>1435
                     /locus_tag="JQH22_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208450.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVVKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          340 a          399 c          397 g          299 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggc ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcgctggtt gtgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttacctg
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgc
//
LOCUS       DADLDT020000309         1405 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000309 DADLDT020000000
VERSION     DADLDT020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.317"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..168
                     /locus_tag="JQH22_004394"
     CDS             <1..168
                     /locus_tag="JQH22_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFB2208451.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            153..791
                     /locus_tag="JQH22_004395"
     CDS             153..791
                     /locus_tag="JQH22_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFB2208452.1"
                     /translation="MGDSVTPETLGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..>1405
                     /locus_tag="JQH22_004396"
     CDS             1019..>1405
                     /locus_tag="JQH22_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208453.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          394 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgatttct ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ctctgtgaca ccggagacac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat gcaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa gatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagt
//
LOCUS       DADLDT020000310         1399 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000310 DADLDT020000000
VERSION     DADLDT020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.318"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..161)
                     /locus_tag="JQH22_004397"
     CDS             complement(<1..161)
                     /locus_tag="JQH22_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFB2208454.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
     gene            complement(1001..>1399)
                     /locus_tag="JQH22_004398"
     CDS             complement(1001..>1399)
                     /locus_tag="JQH22_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFB2208455.1"
                     /translation="NAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLR
                     LMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGH
                     SSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          391 a          317 c          296 g          395 t
ORIGIN      
        1 ttaagttctt cgagcatctt acgaatatca cgaaggacat ttcggtgctc cttgcctgtc
       61 agctcagcaa tttcaactga ggtcatggac acaagctggt cgaggttaat gatgctgatt
      121 tcttgttgtg gttgttgtgg ttgttgtggt tgttgagaca tcgcttgatt tcctttttgt
      181 ttgcaccctg aagtcgcacc cccactgacc tactcaaagt ccccaagcga agggagacca
      241 gcaggacaac aggatgcaaa cagaatgact ttgagattga atactatgat gatgacgatg
      301 tagctgtgcg gagctactgg gagggtctct ctaggagcca ctctagggtc tctctaggca
      361 tcaggctata cgaaactaca gggcatccta aagaatgacg tacagggcca tagaggacca
      421 tagagggcca tagagttagc ttctgggggt aatgcctaga ggagacccta gagagaccta
      481 tagagagacc ctagagaaga cctataggtt tggtgattga ttattataaa tatcatcacc
      541 ccttaaggta ttcccttaga gttaatcttt aattggtctt taattaaatc ttttatttaa
      601 tacttaaagt taatctctaa ttgtgtcttt aagttaactt taagtaatac tataagttaa
      661 tctttaattg tgtctttaag ttaactttaa gttaagcccc tgtagggact agagaccata
      721 attaagatga ttgtgtctta ttgctattca cttatcgact gacccagagc acacaggaag
      781 cattcacttt aagtagaacg cttggtgtct gtaattggtc atcccgcccc tgaaaggatg
      841 cgataagtta cggttagttg tcctagaaga gaaaactttt tgtgtccctt ctccctatag
      901 tgagtcgtat taattatccc ttgataagta gagaaaacta tgagtcaaaa acatgaccac
      961 acaggtctgt agtaagtgtc atcctagact atggtctcct ctaggtctcc ttataaacat
     1021 cagccatcac attgaccagc ctatcaacct cgacctcttt agcataacgg tcgtacgtaa
     1081 tagaccctga actatgcccc atgatcgcct gtgcatacgc caactcgacc ccatgttgtt
     1141 ttaactgggt cgcaactgta tgtctaaacg agtgaaacgc taagccgcta cggtctacga
     1201 ggactttagg caacaacgtc cgattaaacc atttactcac ctgctcaccg taaccgttct
     1261 tcatgagacg cagaccatcg aacaccatag cgtcatcacc gtcagcgcct ctacgggtct
     1321 ctacaagcgc cataaagtca gccaatataa aaccataggc cccatcgacc agaggcacac
     1381 agcggtcact atgagcgtt
//
LOCUS       DADLDT020000311         1380 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000311 DADLDT020000000
VERSION     DADLDT020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.319"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..253
                     /locus_tag="JQH22_004399"
                     /pseudo
     CDS             1..253
                     /locus_tag="JQH22_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1380
                     /locus_tag="JQH22_004400"
     CDS             746..>1380
                     /locus_tag="JQH22_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFB2208456.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          320 c          421 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
//
LOCUS       DADLDT020000312         1356 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000312 DADLDT020000000
VERSION     DADLDT020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.320"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(3..485)
                     /locus_tag="JQH22_004401"
     CDS             complement(3..485)
                     /locus_tag="JQH22_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2208457.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="JQH22_004402"
     CDS             complement(476..778)
                     /locus_tag="JQH22_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFB2208458.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="JQH22_004403"
     CDS             1050..1322
                     /locus_tag="JQH22_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208459.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DADLDT020000313         1335 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000313 DADLDT020000000
VERSION     DADLDT020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.321"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            433..1182
                     /locus_tag="JQH22_004404"
     CDS             433..1182
                     /locus_tag="JQH22_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFB2208460.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          375 a          274 c          272 g          414 t
ORIGIN      
        1 ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta cccgtaaata
       61 ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact caggctacct
      121 caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga gtcattgtct
      181 gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa cttaattcta
      241 actgtttgtt ctgggtgttt taaacgtcct cctttttatg cttgttcata acggatattt
      301 aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat agcacacaca
      361 attttattat ccagtacttt atactcttaa taaaataatt ataaatattt atcctctata
      421 aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt attttcttta
      481 aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga cctttactac
      541 ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc acaaatcgga
      601 agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt gcgaaatact
      661 gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt ggcaaatgta
      721 ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt gcataaatta
      781 ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa agaatattat
      841 atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa agaacaggat
      901 acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg ggcaacagca
      961 cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg gggttatgaa
     1021 ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga cgcagcacgt
     1081 attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca ggcacaatta
     1141 caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta gccagtacgc
     1201 cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact ggctcttttt
     1261 cagggctgtg gggtatttcc agggcctctc ctttaccact ctcggaaagg ccctttccct
     1321 tcttgtcggt aattt
//
LOCUS       DADLDT020000314         1325 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000314 DADLDT020000000
VERSION     DADLDT020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.322"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            116..913
                     /locus_tag="JQH22_004405"
                     /pseudo
     CDS             116..913
                     /locus_tag="JQH22_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          401 a          239 c          307 g          378 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga tggtttatct ggtgggttaa ttgatggtct
      901 gaagatgctt gctgtggctg ttactgctca aaaggctgca aatgtagcag gaggcttgtt
      961 aaaccttgca aacgctgtta cttcattcgg tgacgctgtt aataaagcta aagctggcaa
     1021 ggctggttct tcaggtggtg cgctaaataa agtcggtaga gttgtccttt atgctgagtt
     1081 ggtaaatttt gctcttgata atttaaaccc tgaagcagct aagaatccga acttacatca
     1141 atcaggtatt ggcacagaaa caaacttcaa ccctgaatac aaaggtgatt catctaagcc
     1201 aatagttcta tcgctgactg gtttaatggt gcaactaata aaattgctaa cttccaagaa
     1261 ccgatgaata acttcgtaaa taacacacaa tcaactccat cagctatcac aggtgacatg
     1321 gttaa
//
LOCUS       DADLDT020000315         1316 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000315 DADLDT020000000
VERSION     DADLDT020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.323"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..171)
                     /locus_tag="JQH22_004406"
     CDS             complement(<1..171)
                     /locus_tag="JQH22_004406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208461.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1316
                     /locus_tag="JQH22_004407"
     CDS             308..>1316
                     /locus_tag="JQH22_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFB2208462.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TL"
BASE COUNT          346 a          324 c          378 g          268 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttat
//
LOCUS       DADLDT020000316         1314 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000316 DADLDT020000000
VERSION     DADLDT020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.324"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(102..1253)
                     /locus_tag="JQH22_004408"
     CDS             complement(102..1253)
                     /locus_tag="JQH22_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFB2208463.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          313 a          306 c          287 g          408 t
ORIGIN      
        1 cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca
       61 ttgaaatgga gaatgaaata ttaaaaaagg ctactgtaga ttcaatctgt caatgcaaca
      121 cccctttcaa ttatctcttt cggtgttttg aacttcagtg tctttctcgg tctgttgttt
      181 agctgagcag caaccagatc tagttcatgt tgagtatatt gggcaagaca tgtcttttta
      241 ggaaagtact gccgaattag cccatttgtg ttctcatttg ttccccgctg ccaaggactc
      301 tgaggatcgc agaagtaaac tttaacgccg gtgctgacag taaattctag atgtctggcc
      361 agttccattc ctctgtccca tgtcagtgat tttctgagtt ctgacggtaa actcaggaat
      421 ttgtcggtaa gagcctgatt tactgagaca gaatctttgc ccctgagtct gaggatgatc
      481 gtataacgtg attttcggtc tacaagtgtg gctatatgag agttttttgt acctgagact
      541 aaatcgccct cccaatgccc cagagagcgt ctgttatcga tatttcggga acgttcgtga
      601 attggtgttc cgttcactat gttaatcgta cctctttcgc ctttgcgggt atgacgcctg
      661 ccatggcgaa ggctatgcga ccgtcgcaga tgctgtatat tcaggtggtg tagcgcttca
      721 cggctacgaa agtacagcgt tttataaatt gtctcaggtg atattcgcag cgttttttga
      781 cgtggttttg ttcgccttaa ccatcctgat atttgctctg gagaccattt catctccagc
      841 ttttccagaa caagctttcg caatggtaaa ttttgatcca gtaagcacgg ttttggcctt
      901 ttcgccattc tgttggctcg gttattagca tcaacagctt tgtaatagcg tctgccccga
      961 ttacgctgaa cttcacgtga gatcgtcgaa ggactgcgat tcagcgcagt agctatcgca
     1021 cgaatgctca ttttggctga caaaccagct cgtatctcct cgcgctcaga cagtgtcagg
     1081 tgagctacag cccgcttacg ctcatggggt tttatgccgc cagtatccct taacatagtg
     1141 aagatcgttc cgggttttga acccaggata ttcgctattt cactgaagcc tgttccgttc
     1201 ttccatagtt caaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa
     1261 acctccgcaa ccccatgttt tcacataact gttgcgttga ccaattgaat ctac
//
LOCUS       DADLDT020000317         1314 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000317 DADLDT020000000
VERSION     DADLDT020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.325"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            119..1132
                     /gene="intI1"
                     /locus_tag="JQH22_004409"
     CDS             119..1132
                     /gene="intI1"
                     /locus_tag="JQH22_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFB2208464.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT          241 a          404 c          393 g          276 t
ORIGIN      
        1 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
       61 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
      121 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
      181 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tccactgggt
      241 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
      301 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
      361 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
      421 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
      481 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc agcttctgta
      541 tggaacgggc atgcggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga
      601 tcacggcacg atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgttacc
      661 cgagagcttg gcacccagcc tgcgcgagca gctgtcgcgt gcacgggcat ggtggctgaa
      721 ggaccaggcc gagggccgca gcggcgttgc gcttcccgac gcccttgagc ggaagtatcc
      781 gcgcgccggg cattcctggc cgtggttctg ggtttttgcg cagcacacgc attcgaccga
      841 tccacggagc ggtgtcgtgc gtcgccatca catgtatgac cagacctttc agcgcgcctt
      901 caaacgtgcc gtagaacaag caggcatcac gaagcccgcc acaccgcaca ccctccgcca
      961 ctcgttcgcg acggccttgc tccgcagcgg ttacgacatt cgaaccgtgc aggatctgct
     1021 cggccattcc gacgtctcta cgacgatgat ttacacgcat gtgctgaaag ttggcggtgc
     1081 cggagtgcgc tcaccgcttg atgcgctgcc gcccctcact agtgagaggt agggcagcgc
     1141 aagtcaatcc tggcggattc actacccctg cgcgaaggcc atcggtgccg catcgaacgg
     1201 ccggttgcgg aaagtcctcc ctgcgtccgc tgatggccgg cagcagcccg tcgttgcctg
     1261 atggatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgca
//
LOCUS       DADLDT020000318         1299 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000318 DADLDT020000000
VERSION     DADLDT020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.326"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            82..1275
                     /locus_tag="JQH22_004410"
     CDS             82..1275
                     /locus_tag="JQH22_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFB2208465.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          387 a          273 c          298 g          341 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctca
//
LOCUS       DADLDT020000319         1271 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000319 DADLDT020000000
VERSION     DADLDT020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.327"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            482..>1271
                     /gene="uacT"
                     /locus_tag="JQH22_004411"
     CDS             482..>1271
                     /gene="uacT"
                     /locus_tag="JQH22_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFB2208466.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          294 a          292 c          279 g          406 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggattaatgt ccaacgtcgc cgtattactg gggattgtat ttggcttttt
     1141 actttcgtgg atgatgaatg aagtcaattt atccgggcta catgatgcct cgtggtttgc
     1201 gattgtcacc ccgatgtcgt ttggtatgcc gattttcgat cccgtttcca ttctgaccat
     1261 gactgccgtg t
//
LOCUS       DADLDT020000320         1259 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000320 DADLDT020000000
VERSION     DADLDT020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.328"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            34..435
                     /locus_tag="JQH22_004412"
     CDS             34..435
                     /locus_tag="JQH22_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFB2208467.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JQH22_004413"
     CDS             447..818
                     /locus_tag="JQH22_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208468.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="JQH22_004414"
     CDS             805..>1259
                     /locus_tag="JQH22_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208469.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DADLDT020000321         1236 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000321 DADLDT020000000
VERSION     DADLDT020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.329"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..890
                     /locus_tag="JQH22_004415"
     CDS             <1..890
                     /locus_tag="JQH22_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2208470.1"
                     /translation="DLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLR
                     AQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFIIYGPQL
                     ISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVISRSVIC
                     MPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEKFMNRK
                     ISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLYISYNK
                     SSLNNLVFSRFIDRLNESF"
     gene            1037..1236
                     /locus_tag="JQH22_004416"
                     /pseudo
     CDS             1037..1236
                     /locus_tag="JQH22_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          381 a          272 c          211 g          372 t
ORIGIN      
        1 acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
       61 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
      121 tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg
      181 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
      241 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc
      301 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
      361 ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg
      421 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
      481 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
      541 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atagccaaat
      601 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
      661 catttcgtgg ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg
      721 gaatagtccc ctatgaactt tataactacc accgtgactt tcttaattta aaagagataa
      781 aactagaaca tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa
      841 ataatttggt gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta
      901 tagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa taaaacactc
      961 tgctttacag tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt
     1021 cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata ttctggaagc
     1081 tgcacgagag cgtattacct agacactcaa caccctaccc cgcgtttgtg tctcattttc
     1141 tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg
     1201 caaaaaaatc tgcgttttgt ttatcaactg ggaggc
//
LOCUS       DADLDT020000322         1225 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000322 DADLDT020000000
VERSION     DADLDT020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.330"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="JQH22_004417"
     CDS             complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="JQH22_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFB2208471.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLST"
BASE COUNT          349 a          220 c          264 g          392 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatgttaa cattcgtaca aatgggcaat gtgattatat
       61 tttcgggtaa tgaagttagt aggttattgg aaacatccag tgccaatagt tcttgcggta
      121 atctggataa tctgcgtagt tgattttcac ctaccttcaa atattttaag ctctgaggaa
      181 attctggtag ttcctgtagt ctgttgccgg aaacaaagag ttcttgtagt ctatgaggca
      241 attcaggcaa gacagtaagg cgattacctt caatacgtaa tattgttagc gttgatggaa
      301 gctctggtaa cgtctccaga ctattaaaac gtgccgagag tgattgtaaa tatggtggta
      361 atgatggcag acatgacaaa tttcgattga atgaaatatt aagcttaatc agttgagatg
      421 gaagtgtcgg gagtatattc aagttgttac catttacatg aagttctgtc aaaagcggag
      481 aattttcagg tagggagatt aactcattgt tggaaatatt aagttcacgt atatgcaaag
      541 ggagtggtgg taaagaacgt agtttcagta agcttaaatt gagaacaggc tcccgggtat
      601 ccagacatat tttcaataat cgaaccgcct ggatgcgatt ttctgttgtt gttccttccc
      661 tagcccagtc atcccagatg cggtggtaat ctctggtaca ctgttcttct accgataaac
      721 gagatataca tgataagcca caaagggaac gaaaaaaaga atgattgttg atcggtttca
      781 tttattaacc cctcactatt catatcttgg tctattaatg caaataaaac gagaaagtct
      841 ttaaaaaaac aatcctagaa ctttatcggc gttttcattc cggctggagg aatatgttcc
      901 tgtctttcca gtaaccgtcg attgttatat cagtctaccc atgttaatgt gaccagttct
      961 actttcatcc agtttggatt gatctttatc ccctatattt tcaaacaatt tttatcattt
     1021 aactcattac tgtctcgata ttcccatagc aaacgatctg gttggttatc ccctcaaaaa
     1081 agtcagttgc acgccgtaaa tccctgccgg tattgagcga ggatttacgg gttgttgtta
     1141 aggtccgtaa tcgggtcaca gtttttccgg agtcaatccg gtctgcggtt tatgcttatg
     1201 cgacgtgatt atgaatggtg cagtc
//
LOCUS       DADLDT020000323         1202 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000323 DADLDT020000000
VERSION     DADLDT020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.331"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>1202
                     /locus_tag="JQH22_004418"
     CDS             join(<1..299,299..>1202)
                     /locus_tag="JQH22_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFB2208472.1"
                     /translation="PEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWR
                     KQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRGKK
                     VDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHH
                     VIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCL"
BASE COUNT          295 a          294 c          365 g          248 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg
      781 cactgggcgg tgactaccgg cggcttcaac agtgaaacag tacaggacgt catgctggga
      841 gcggtggaac gccgcttcgg caacgatctt ccgtcgtctc cagtggagtg gctgacggat
      901 aatggttcat gctaccgggc taatgaaaca cgccagttcg cccggatgtt gggacttgaa
      961 ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag cttcgtgaaa
     1021 acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
     1081 aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc gctgggttat
     1141 cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga taacagatgt
     1201 ct
//
LOCUS       DADLDT020000324         1195 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000324 DADLDT020000000
VERSION     DADLDT020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.332"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..154
                     /gene="relE"
                     /locus_tag="JQH22_004419"
     CDS             <1..154
                     /gene="relE"
                     /locus_tag="JQH22_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFB2208473.1"
                     /translation="GMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSE
                     AVKRIL"
     gene            226..381
                     /gene="hokD"
                     /locus_tag="JQH22_004420"
     CDS             226..381
                     /gene="hokD"
                     /locus_tag="JQH22_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFB2208474.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            599..814
                     /locus_tag="JQH22_004421"
     CDS             599..814
                     /locus_tag="JQH22_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208475.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            917..1195
                     /locus_tag="JQH22_004422"
                     /pseudo
     CDS             917..1195
                     /locus_tag="JQH22_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          319 a          277 c          300 g          299 t
ORIGIN      
        1 tggtatgcct gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca
       61 ggttatagac gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga
      121 agtatatagc gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc
      181 accgaaggtg acacttctgc tttgcgttga caggagaagc aggctatgaa gcagcaaaag
      241 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
      301 aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc tgtcttcaca
      361 gcttacgaac ctgaggagta agagacccgg cgagtgagaa atccctcgcc acctctgatg
      421 tggcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt gtttttattt
      481 tcaacgcatt tgaagttctg gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
      541 cagggataaa agggatggac cccgaacagg ggagtgctac ttatctggaa ggattctgat
      601 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctttatg ctgtgtgcgt
      661 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
      721 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
      781 tgtcacttca ttacaggcat tgaaagagat ctgaataaaa gctggctttt ctgctttttc
      841 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
      901 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aatcatgata attcgaccgt
      961 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1021 ctcatgcact gaagaacagg ccgaatggct tattcagtgt taccgcaggc gcggatgcga
     1081 ggttaaaaaa gcccttagcc tcgactaccg tcactagata atctccgtca ggctccctta
     1141 ctccgaacag ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaa
//
LOCUS       DADLDT020000325         1163 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000325 DADLDT020000000
VERSION     DADLDT020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.333"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(371..>1163)
                     /locus_tag="JQH22_004423"
     CDS             complement(371..>1163)
                     /locus_tag="JQH22_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFB2208476.1"
                     /translation="SIRQACRSLNLSRTVYHYRPDTTRDEPVIVALQAVAERYPRYGF
                     PKLFQVLRRQGYPWNHKRIHRIYCLLKLNFRRKGKQRLPVRNPSPLATSEALNQSWSV
                     DFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPALRVVRVLDRIAANRGYPVKLR
                     MDNGPEFISLTLAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLN
                     EVREITEKWLSEYNCERPHESLNNMTPEEYRQHHYLAGISKNAWN"
BASE COUNT          323 a          275 c          267 g          298 t
ORIGIN      
        1 cgctcatggg cacctctctt taagccatct taaatgactc tgaggtgtct gttaaacccg
       61 tggcgattca gccaatataa atcgctgcaa caataaacgt aaacctgcga ttatacctcc
      121 tagcctagga tattagacgg taagagccaa aagacacaaa aaatgtaaaa aactgtaggt
      181 gttctcatgg ttcattacaa acgttgaata tacaatgcaa ccaatgtcat ttgagcagta
      241 aaaaacatga atagcgtaca aatagaagaa aatgttaaga gcttggtaaa acaagtatca
      301 agccaagaaa tctcaaaaga tgattttatc tacgagctat tgttggctta cgggtgtaaa
      361 tagacccgtt ttagttccag gcattttttg agatcccggc caaataatgg tgttgtcgat
      421 attcctctgg cgtcatattg ttcagtgatt catgcgggcg ttcacagtta tattctgaca
      481 accatttttc cgtgatttcc cgcacttcat tcagcgttct gaacagataa aaatcgagta
      541 tttctgtacg gtatgtccgg ttaaagcgct caatgaaagc gttctgcgtc ggcttacccg
      601 gctggataaa ctccagtttt actgcatgtt tctctgccca ttcagccagt gtcagggaga
      661 taaattccgg accattatcc atgcgcagct tgaccggata gccacggttt gccgcgatcc
      721 tgtcgagtac acggaccacg cgtagagctg gcagattcag gtcgatttca atcgacaacg
      781 cctcacggtt aaagtcatca acgacattga acgtacgaaa acgacggcca cagaccaggg
      841 catcatgcat aaaatcgaca gaccaactct ggttcagcgc ttccgacgtg gccagtggcg
      901 agggattacg caccggcaac cgttgtttgc ccttacggcg aaaattcagc ttcagcagac
      961 agtaaatacg gtggatcctt ttgtgattcc atgggtatcc ctgtcgccgc agaacctgga
     1021 agagtttcgg aaaaccgtat cgtgggtatc gctctgccac tgcctgcaac gcgacaataa
     1081 cgggttcatc acgcgtggta tccgggcggt aatggtaaac cgttctgctc aggttcagac
     1141 tccggcaggc ctgacggata ctg
//
LOCUS       DADLDT020000326         1160 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000326 DADLDT020000000
VERSION     DADLDT020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.334"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="JQH22_004424"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="JQH22_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFB2208477.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          299 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcagctatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DADLDT020000327         1155 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000327 DADLDT020000000
VERSION     DADLDT020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.335"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            2..385
                     /locus_tag="JQH22_004425"
     CDS             2..385
                     /locus_tag="JQH22_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFB2208478.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JQH22_004426"
     CDS             complement(386..1030)
                     /locus_tag="JQH22_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2208479.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcattgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADLDT020000328         1138 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000328 DADLDT020000000
VERSION     DADLDT020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.336"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..141)
                     /locus_tag="JQH22_004427"
     CDS             complement(<1..141)
                     /locus_tag="JQH22_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFB2208480.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
     gene            complement(153..>1138)
                     /gene="yiaK"
                     /locus_tag="JQH22_004428"
     CDS             complement(153..>1138)
                     /gene="yiaK"
                     /locus_tag="JQH22_004428"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFB2208481.1"
                     /translation="EQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSHGVNRF
                     PRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIG
                     LVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPS
                     TPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGY
                     WKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKL
                     QRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
BASE COUNT          260 a          300 c          313 g          265 t
ORIGIN      
        1 cgcataaata ttcttgccgt cgatttcgac aacgcccggc tccaggacgt tgaagttggt
       61 agcgcgcaga aaatcgagcg ccttttcaat ggcggcgggc aaacggcacg gattgggctg
      121 cgcgatatgt ccaaaaatca tgacttatct cctcataacg cctggatttt ggcccacacg
      181 ctgtcatcaa cagtgatgcc gttacggcgg ttttcggcca gcagggtagt aaattcgtgg
      241 ccgggtaagc ggatggcctg gttttcgtca gcgcgttcag cggtagtaac gtaatccatg
      301 atacgttgca gcttggcatc gcgggtggga ccgtcgataa gcttgtccac ttcaatggca
      361 ataaaaattt gtgaaatgcc gtattcgtcg ctgttgtcct gggtgacttc ggcaaccgat
      421 gcgccgtcgg aaaggagagt agcgatcata tccagcacaa tcgacatgcc agaacctttc
      481 cagtagccca tcggcaaaat gcggcgattc ttctcgataa cgccaggttc tttggtcaaa
      541 ttgccctcat catcaaagcc accatcgacc gggagctgac ggcctgccag gcggttaact
      601 tctaacatgc cgtaagagaa catcgacatc gacatatcga ccatggtgat cggcgtggaa
      661 ggaatggcga cgatcagcgg gttagtgccg atgcgacact cttttgcgcc ccacggcggc
      721 attacggcga tggagttggt ccagcaaatg ccaatatagc ctttttccgc cgcctgccag
      781 ccgtagctgc cgccgcgcat ccagtggttg gcattacgaa gtgccaccag accaataccg
      841 tgatcggcag ccagttcaat ggcgcgatcc atcatctttt tcgccgtcag gttgccgatc
      901 gaacgctggg catcccactg ttcaattgcg ccgaggctgg ttatacgttt gggttgggca
      961 tcaggaatga tatcgccgtt ttccagttgt tgaatgaaac gagggaaacg attaacgccg
     1021 tgagaataaa cgccggattc ggtggtgcgg gcgaacatct ctgcacaggc gtcagccgtt
     1081 tcgctgtcaa cgccgcgtga aattaagacc cgattaaagg ctgcttttaa ctgctcaa
//
LOCUS       DADLDT020000329         1125 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000329 DADLDT020000000
VERSION     DADLDT020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.337"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..114
                     /locus_tag="JQH22_004429"
     CDS             <1..114
                     /locus_tag="JQH22_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208482.1"
                     /translation="GPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            111..512
                     /locus_tag="JQH22_004430"
     CDS             111..512
                     /locus_tag="JQH22_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFB2208483.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            523..>1125
                     /locus_tag="JQH22_004431"
     CDS             523..>1125
                     /locus_tag="JQH22_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFB2208484.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSD"
BASE COUNT          284 a          279 c          328 g          234 t
ORIGIN      
        1 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
       61 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
      121 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
      181 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
      241 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
      301 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
      361 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
      421 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
      481 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
      541 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
      601 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
      661 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
      721 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
      781 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
      841 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
      901 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
      961 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     1021 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     1081 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagat
//
LOCUS       DADLDT020000330         1102 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000330 DADLDT020000000
VERSION     DADLDT020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.338"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            52..>1102
                     /locus_tag="JQH22_004432"
     CDS             join(52..301,301..>1102)
                     /locus_tag="JQH22_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HFB2208485.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          303 a          273 c          310 g          216 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg ga
//
LOCUS       DADLDT020000331         1068 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000331 DADLDT020000000
VERSION     DADLDT020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.339"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            18..560
                     /locus_tag="JQH22_004433"
     CDS             18..560
                     /locus_tag="JQH22_004433"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208486.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="JQH22_004434"
     CDS             787..>1068
                     /locus_tag="JQH22_004434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFB2208487.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DADLDT020000332         1066 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000332 DADLDT020000000
VERSION     DADLDT020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.340"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(570..749)
                     /locus_tag="JQH22_004435"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="JQH22_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="JQH22_004436"
     CDS             843..>1066
                     /locus_tag="JQH22_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208488.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DADLDT020000333         1040 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000333 DADLDT020000000
VERSION     DADLDT020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.341"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..769
                     /locus_tag="JQH22_004437"
     CDS             <1..769
                     /locus_tag="JQH22_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFB2208489.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1040
                     /locus_tag="JQH22_004438"
     CDS             790..>1040
                     /locus_tag="JQH22_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFB2208490.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          337 a          231 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag
//
LOCUS       DADLDT020000334         1030 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000334 DADLDT020000000
VERSION     DADLDT020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.342"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>1030
                     /locus_tag="JQH22_004439"
     CDS             <1..>1030
                     /locus_tag="JQH22_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFB2208491.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAE"
BASE COUNT          319 a          193 c          269 g          249 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccgt ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 gagtgcggaa
//
LOCUS       DADLDT020000335         1020 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000335 DADLDT020000000
VERSION     DADLDT020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.343"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(50..190)
                     /locus_tag="JQH22_004440"
     CDS             complement(50..190)
                     /locus_tag="JQH22_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208492.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="JQH22_004441"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="JQH22_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFB2208493.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          281 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DADLDT020000336         1003 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000336 DADLDT020000000
VERSION     DADLDT020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.344"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..87
                     /locus_tag="JQH22_004442"
     CDS             <1..87
                     /locus_tag="JQH22_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFB2208494.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..524
                     /locus_tag="JQH22_004443"
     CDS             102..524
                     /locus_tag="JQH22_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFB2208495.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            582..938
                     /locus_tag="JQH22_004444"
     CDS             582..938
                     /locus_tag="JQH22_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208496.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          288 a          201 c          276 g          238 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccga taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta taagccatcg gaaagaccat attgccatca cgcctctgta cgggttgacg
      721 aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga
      781 tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg
      841 aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag
      901 cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa
      961 tttaatcctt aaccggaggg atttctgcac cctcaaatca tca
//
LOCUS       DADLDT020000337          995 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000337 DADLDT020000000
VERSION     DADLDT020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.345"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(174..863)
                     /locus_tag="JQH22_004445"
     CDS             complement(174..863)
                     /locus_tag="JQH22_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HFB2208497.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          244 a          264 c          193 g          294 t
ORIGIN      
        1 ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgaccacc agcacgttta
       61 acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg cggcgggttt tgcttacttt
      121 accatcgcgt acaaaatcgg caaagtatca gatttgcatg agatatatgc cttttaatct
      181 acttttgcaa tactttgctt tgaaaatgcc gctttttgtt ctgaacgtgt tctcatcaca
      241 aacaataaag cctcactatc cagtcggtga aaaatgtgtt tcattacaac ccagtgacca
      301 gtaaatgttt tggaccagtt tttggttgtt actcccgcca gtaatgccag ctcctgatat
      361 tcataacctt ccccaccaaa aagttctgct tttaccgcct gcgccgccag ccagattaat
      421 tttttcaggc gttcctgtgt tttctctgca attttcctag tgccagaatg agccttaaac
      481 tcttcccacg cccagtgggt tatggtgatc tgatactccc agcaaatact tccgctgtaa
      541 caccacaaca accaggcttt atagtgctct tcaagagaca gaacagcccg ccgccacgat
      601 gatgtcgaaa acgcaacctg actgaccaga ggaattgacg cccccttcgc cagtgattgt
      661 tttcccggga ttggtggatt atcccgcgtt atcatttttc cggtcacctc atcgcggtac
      721 cggatttttt tacgcctgta acgccctgta tcgaacatgg cattctcctg ccaggcttca
      781 agctgacctt ttgtcgcccc actcaaatca gcggtggcga taatgagctg ctcacgaaca
      841 aactgtaaat actggttatt catgcacact ccagctctgt gatttttatc ctgaagtggt
      901 caacaaaaac tggccaccga gttagagttt ttccagtatc gattttccga ttcgtttggg
      961 ggtaacccac cgttatattc gtgcggtctt agtgc
//
LOCUS       DADLDT020000338          984 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000338 DADLDT020000000
VERSION     DADLDT020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.346"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQH22_004446"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQH22_004446"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFB2208498.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          260 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataa
//
LOCUS       DADLDT020000339          946 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000339 DADLDT020000000
VERSION     DADLDT020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.347"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            57..554
                     /locus_tag="JQH22_004447"
                     /pseudo
     CDS             57..554
                     /locus_tag="JQH22_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            595..786
                     /locus_tag="JQH22_004448"
                     /pseudo
     CDS             595..786
                     /locus_tag="JQH22_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          246 a          236 c          267 g          197 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcatca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgc aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat gacttcacgt tcgccaccgg agcaccgcag
      721 aagcagctcc agtcgttacg ctcactcagc ttcatagagc gcaatgaaaa catagtctta
      781 ctggggacat ctgacatcac aaatccccgg gtggggattt gtgtataaga gacagcccat
      841 ggaggaagga gatatatcgc agctctgacg actcactcct tatttacccg ggaaatactt
      901 ctccagcaat gcatacagga tggcagcggt ttcagcatcc attatg
//
LOCUS       DADLDT020000340          933 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000340 DADLDT020000000
VERSION     DADLDT020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.348"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
BASE COUNT          245 a          180 c          217 g          291 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc atctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DADLDT020000341          932 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000341 DADLDT020000000
VERSION     DADLDT020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.349"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..932
                     /locus_tag="JQH22_004449"
                     /pseudo
     CDS             1..932
                     /locus_tag="JQH22_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
BASE COUNT          225 a          262 c          239 g          206 t
ORIGIN      
        1 cgtgggtgcg ttttttgcgg gtgagaacgg taactccacc actggcgacg ccatttatat
       61 gcgtggtgcc gatacctcta acagtattta tattgatggt attcgcgata tcggcagcgt
      121 ctcgcgcgac accttcaata ccgagcaggt cgaagtgatt aaagggccgt ccggcaccga
      181 ctacgggcgc agcgcaccga caggctcgat caatatgatc agcaagcagc cgcgcaatga
      241 ttccggcatt gacgcctccg ccagtattgg cagcgcctgg ttccgccgcg gcacgctgga
      301 cgtcaatcag gtcattggtg atactaccgc ggtgcgcctg aatgtgatgg gcgaaaaaac
      361 ccacgatgcc ggacgcgaca aagtcaaaaa tgagcgttac ggcgtcgccc cttctgtcgc
      421 ttttggcctt ggtacagcaa atcgtttgta tcttaattat ctgcatgtca ctcagcacaa
      481 cacgccagac ggcggcattc cgaccatcgg tttgccgggc tattctgccc catctgcggg
      541 agcggcggcc ctgaatcatt ccggaaaagt tgatactcat aacttttacg gcacggattc
      601 cgattacgac gattcgacca ccgacaccgc caccatgcgt tttgagcacg acatcaacga
      661 taacaccacc attcgcaata ctacccgttg gtcgcgcgta aagcaggatt acctgatgac
      721 ggcgattatg ggcggggcgt cgaatattac tcagcccacc agcgatgtga atagctggac
      781 ctagtcacgc acggcgaata ccaaagatgt gagtaataaa attctcacca accagaccaa
      841 cctgacctcg acgttctata ccggttctat cggtcatgat gtcagtaccg gcgtggaatt
      901 tacccgtgaa acgcagacta actatggcgt ta
//
LOCUS       DADLDT020000342          927 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000342 DADLDT020000000
VERSION     DADLDT020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.350"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..927
                     /locus_tag="JQH22_004450"
                     /pseudo
     CDS             1..927
                     /locus_tag="JQH22_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          214 a          234 c          301 g          178 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgta
//
LOCUS       DADLDT020000343          921 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000343 DADLDT020000000
VERSION     DADLDT020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.351"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>921
                     /locus_tag="JQH22_004451"
     CDS             <1..>921
                     /locus_tag="JQH22_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFB2208499.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLV
                     KLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSNRW
                     EIELGYREIKQTMQLSRLTLRSKKPELVEQ"
BASE COUNT          239 a          250 c          266 g          166 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      541 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      601 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      661 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      721 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      781 catgcgcttc cccggaggag aaatggcgga tctgtacagt aatcgctggg aaatcgaact
      841 gggatacagg gagataaaac agacgatgca actgagcagg ctgacgctga gaagtaaaaa
      901 gccggagctt gtggagcaag a
//
LOCUS       DADLDT020000344          917 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000344 DADLDT020000000
VERSION     DADLDT020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.352"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            25..569
                     /locus_tag="JQH22_004452"
                     /pseudo
     CDS             25..569
                     /locus_tag="JQH22_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            593..>917
                     /locus_tag="JQH22_004453"
     CDS             593..>917
                     /locus_tag="JQH22_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFB2208500.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEE"
BASE COUNT          266 a          194 c          235 g          222 t
ORIGIN      
        1 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactac gggtaatccg
       61 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      121 gacggaaaaa actggtatga agagctgaag aatttcgcca ggacacaatt aaaataactt
      181 acaccggaga cggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatt gaaccccgta
      241 acgccagtgt tattgaagtt cctgatatta ccgccaaccg ccgcattacc gcgccgggtt
      301 actggtttta ccgcaatgat gaatttgtct ttgactacag actcaaagcg gaagatgagc
      361 gtgatgccct tctggctcag gtcagtgccc ggacagggga atgggaagaa gacctgttgc
      421 tggggctaat cagcgacgaa gaccgggaaa aactgaaagc gtaccgcatt tacgcgaaat
      481 cgctgcaggc gatggatttc agtgtcatca ctgataaaac ctcatacaac gccattgaat
      541 ggcccgcttc tccggaaatt tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt
      601 agtgatatca ggtgcgctga ctgatggcgc aggtatctcc atgtccggat accatattat
      661 tctgaaatcc cgggtaaaca ccccggaagt ggtgatgcac actgttgctg atgtgatgac
      721 aggaaacgat ggtgaatact gtttccatgc gcggactgga aaatatggtg tgtatctgaa
      781 acaggactgg cacaacgagt acaacgttgg cgacattgct gtatatgagg actcaaagcc
      841 cggtacgctg aatgactttc tgattgctcc tgatgagggc gacctgaaac cagatgtcgt
      901 caaacgcttt gaggaaa
//
LOCUS       DADLDT020000345          910 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000345 DADLDT020000000
VERSION     DADLDT020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.353"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..86)
                     /locus_tag="JQH22_004454"
     CDS             complement(<1..86)
                     /locus_tag="JQH22_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFB2208501.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVA"
     gene            152..910
                     /locus_tag="JQH22_004455"
                     /pseudo
     CDS             152..910
                     /locus_tag="JQH22_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          249 a          195 c          215 g          251 t
ORIGIN      
        1 gcaacgctgg caacagaata accgcgatca acaacctgtt tgactgcttc agttttaaac
       61 tcttcgggat aacgcttacc gctcatgggc acctctcttt aagccatctt aaatgactct
      121 gaggtgtctg ttaaacccgt ggcgattcag tgagcgcatt ctgaccgacc gtgaaaatgg
      181 tgaaaaaggc gataattctc aagatacctc ttatgcccgt gtaggcgtga aaggtgaaac
      241 tcagatcaac cctgaaatga ctggttacgg tcagtttgaa ctagacctgg aagcatcaaa
      301 cagacacaac cctgaccaaa ctcgtttggc atatgctggt ttgagttaca aagattttgg
      361 ttcattcgac tacagtcgta atgtcggtgt cgcgtatgat gcagaagcat ttactgatat
      421 gtttgttgag tggggcggtg actcatgggc tggtactgac ctgttcatga ccaaccgtac
      481 caacggtgtt gcaacctatc gtaacacaga tttctttggc atggttgaag gtctaaattt
      541 tgctctgcaa tatcagggta aaaatgaagg tacaggcaac tacaaagcaa atggcgatgg
      601 ccatggttta tctgcaactt acactattga cggtttctca tttgctggtg cttatgcaaa
      661 ctctgaccgt actgattggc agtctggtga tggtaaaggc gaaagagccg aagtttgggc
      721 gttgtcaaca aaatatgacg ctaacaatgt ttatgctgct gtgatgtacg gtgaatctca
      781 caacatgaat tccgacgacg gtgatgttgt gaacaaaaca caaaacttcg aagctgttct
      841 gcaatatcag ttcgactttg gtctgcgtcc atccatcggt tactcctatt ctaaagcact
      901 ggacgtagca
//
LOCUS       DADLDT020000346          883 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000346 DADLDT020000000
VERSION     DADLDT020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.354"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            9..563
                     /locus_tag="JQH22_004456"
     CDS             9..563
                     /locus_tag="JQH22_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFB2208502.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            704..>883
                     /locus_tag="JQH22_004457"
     CDS             704..>883
                     /locus_tag="JQH22_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFB2208503.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
BASE COUNT          218 a          198 c          247 g          220 t
ORIGIN      
        1 ggttatttat gatttaccca acaaatacag gcaaaagcgg ggaacacctt cgtctcacca
       61 cgctggaaag tgtctggatt cagggaaaac tgcgcatgtg ggggcgctgg tcgtatattg
      121 gcggcggtaa gacgggaaat atgtttaacc agttgctggc atccaaaaaa ttgacgaaaa
      181 cagccgtcaa tgaagccctg cgcaggatga aaaaagcggg aatagagaaa cctgagctgg
      241 aagcgttttt gcgagagatg atcaatggca agcaaaagac ctggctggcg cattgtactg
      301 atgcagaggc gttatgtatt gatagagtca taagtgaggt gctggcagag catcctggat
      361 tgattagtgt cctccggcaa cggtatgaag ggcgggggat gactaagcgt aaaatggctg
      421 aattgctgaa tgacgcacac cctgagtggt gtttccggac gtgctgcagt cgggtagatg
      481 tatggctaaa tcttgctgaa tatatgctct atctaccgat gcgtgatgca ttctcttccg
      541 gggatctaaa aaccgtttgt tgactcaatc tgttatccgg ggctatattc ctcacgcgcc
      601 agcaaaatct ggcgtcggga ttggcgtccc ggatgtttac ggagcgatat gagacgcgcc
      661 cgcgtctttt ttcatatcgt ttgcacagtc acattcgcga tttatggcgg gctgtgtggg
      721 ggagccgaaa ggctcgccgg tttccgtacc cggttacgcc aaccctgcac agttcgccac
      781 cagttcgatt ggcgtcgtcg gtggcgataa tttccaaatg tacggagtta tcgttatgac
      841 cactcaaatc tctgttgaaa ctctctcccc gatcacccat aac
//
LOCUS       DADLDT020000347          866 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000347 DADLDT020000000
VERSION     DADLDT020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.355"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(212..835)
                     /locus_tag="JQH22_004458"
     CDS             complement(212..835)
                     /locus_tag="JQH22_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFB2208504.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          184 a          277 c          200 g          205 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttctctgttc
      121 agcctgaccg cacaccccgg cggccggatg acagactccg cctcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attacttaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagactgctc agctcctctt tcatctcccg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tccgcatcac tccacaccat atgtgtttgt gccgcgacag
      421 attttgccag catgaccacc ggggacaggc gggcaactga cgaggcgtca gcgttccacg
      481 tgcggccgtt ccattcaaac gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa
      541 tttcctgacg tctggcctct ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg
      601 ctatctcacc ccatttgcca ctttgcagtt cctgccagat ttgctgaccc gtcggtgcaa
      661 catcatcagc ggtggctgtg tagggaactg cctggtccct gtcgtcaaaa aaaacgtcac
      721 agtctactgc gccactttcg gtataacggg gattaatgat ttttttaatt tccacggtgc
      781 attcctcacg atgtgcgaat aaaaagcccg ggcattgcgc cagagacatg agcatccggc
      841 accccggaca gggcgcaata tgaccc
//
LOCUS       DADLDT020000348          831 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000348 DADLDT020000000
VERSION     DADLDT020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.356"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..326
                     /locus_tag="JQH22_004459"
     CDS             <1..326
                     /locus_tag="JQH22_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFB2208505.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQH22_004460"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQH22_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFB2208506.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DADLDT020000349          824 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000349 DADLDT020000000
VERSION     DADLDT020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.357"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..112)
                     /locus_tag="JQH22_004461"
     CDS             complement(<1..112)
                     /locus_tag="JQH22_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFB2208507.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="JQH22_004462"
     CDS             complement(109..399)
                     /locus_tag="JQH22_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFB2208508.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>824)
                     /locus_tag="JQH22_004463"
     CDS             complement(399..>824)
                     /locus_tag="JQH22_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFB2208509.1"
                     /translation="FDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          191 a          230 c          185 g          218 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc atgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggttt tgtcgctgtt ccgcacgtcc ccgccagcct
      301 gtaatgcgcc agcacagaag tttcgggatt gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttacgtaa atccaccatt acgcaaactc cagtaactgc
      421 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      541 gcgatagatt ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat
      601 ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa tgccgtatcc
      661 cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact cagggctgga
      721 gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac cagttttcgt
      781 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat caaa
//
LOCUS       DADLDT020000350          811 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000350 DADLDT020000000
VERSION     DADLDT020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.358"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>811
                     /locus_tag="JQH22_004464"
     CDS             <1..>811
                     /locus_tag="JQH22_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFB2208510.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DADLDT020000351          798 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000351 DADLDT020000000
VERSION     DADLDT020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.359"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..128)
                     /locus_tag="JQH22_004465"
     CDS             complement(<1..128)
                     /locus_tag="JQH22_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HFB2208511.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGA"
BASE COUNT          153 a          249 c          195 g          201 t
ORIGIN      
        1 gccccctttg atgaaagcgt gatccgcgcc gcttcagcca gccattctgg ttgtgttatc
       61 agacgcagca gccgttgaat ccgtagggac ggatcggtgg ctgcccatgt ggcaagcttg
      121 cgttgcattt cgctgattat caaaggtctt cacctcgtta ggccagttaa ttcacgtcgc
      181 aaacacattc aaactgcttc ccttcgccat gtaatgggct ttccccatcg cggactacta
      241 cggaagctcc gccagtcagc gcgtcatcgg agccatgccc ccttaacatc cgtcgctgac
      301 cttccccggt ttacctgcct ggactcaggc atactgagga ggctgcccgt cgcactcttt
      361 atccttgctt gccgcaagtt ggcagaagtc agcaacgcaa gcgtgataga cgctgctgcc
      421 ccggtgtttc gcatacatgt caaaacacct tcgaccggca gtgcttacgt atcactgcca
      481 gttcctcctg cacggcctgt cagatcacgt aggccgtggt gacgttttca acccacagag
      541 gcggattaac gggttcatgt tcttcagcct ttcagtactt aaccttgagg atcatctcgg
      601 cttagtgatc tcgcctcaat ccccgttgtc agcgggttac atcaccctgc gggcatgccg
      661 caggtcactg ccgctcaggt tctccaccgt cacacccggt gggattgttg ggtttctcat
      721 cgtgagttac cggttcaata ttccagacag actcgcggtt catttaagca tccatgcccg
      781 ccctgaactc cgggcaca
//
LOCUS       DADLDT020000352          782 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000352 DADLDT020000000
VERSION     DADLDT020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.360"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..321
                     /locus_tag="JQH22_004466"
     CDS             <1..321
                     /locus_tag="JQH22_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208512.1"
                     /translation="RSGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRL
                     SPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSL
                     SEIR"
     gene            325..782
                     /gene="pgaB"
                     /locus_tag="JQH22_004467"
                     /pseudo
     CDS             325..782
                     /gene="pgaB"
                     /locus_tag="JQH22_004467"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT          245 a          168 c          163 g          206 t
ORIGIN      
        1 cgctcaggtg taaacatcta tgcgtggatg ccggtattaa gctgggattt agatcccaca
       61 ttaacgcgag taaaatactt accaacaggg gagaaaaaag cacaaattca tcctgaacaa
      121 tatcaccgtc tctctccttt cgatgacaga gtcagagcac aagttggcat gttatatgaa
      181 gatcttgccg gacatgctgc ttttgatggc atattgttcc acgatgatgc tttgctttca
      241 gattatgaag atgccagtgc accggctatc acggcttatc agcaagcagg ctttagcggg
      301 agtctgagcg aaattcgata aaacccggag caatttaaac agtgggcccg ctttaaaagt
      361 cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg taaaagccat tcgcggtcca
      421 catattaaaa ctgcacgaaa tatttttgca cttccggtaa tacaacctga aagtgaagcc
      481 tggtttgcac agaattatgc tgatttccta aaaagctatg actggaccgc tattatggct
      541 atgccttata tggaaggtgt cgcagaaaaa tcggctgacc aatggttaat acaattgacc
      601 aatcaaatta aaaacatccc tcaggctaaa gacaaatcta ttttagaatt acaggcacaa
      661 aactggcaga aaaatggtca gcatcaggct attccttcgc aacaactcgc tcactggatg
      721 agcctattac aactgaatgg agtgaaaaac tatggttatt atcccgacaa ttttctgcat
      781 aa
//
LOCUS       DADLDT020000353          774 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000353 DADLDT020000000
VERSION     DADLDT020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.361"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..179)
                     /locus_tag="JQH22_004468"
     CDS             complement(<1..179)
                     /locus_tag="JQH22_004468"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="hydrolyzes the beta-lactam bond in antibiotics such
                     as the penicillins and cephalosporins; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase LEN-27"
                     /protein_id="HFB2208513.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRP"
     gene            complement(362..>774)
                     /locus_tag="JQH22_004469"
     CDS             complement(362..>774)
                     /locus_tag="JQH22_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208514.1"
                     /translation="LLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFI
                     DDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVV
                     LTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          205 a          206 c          160 g          203 t
ORIGIN      
        1 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
       61 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac gaaaatgttg aatactcata
      181 ctcttccttt ttcaatatta ttgaagcatt taccagggtt attgtctcat gagcggatac
      241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
      301 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
      361 atcacgaggc cctttcgtct tcaagaattt tataaaccgt ggagcgggca atactgagct
      421 gatgagcaat ttccgttgca ccagtgccct tctgatgaag cgtcagcacg acgttcctgt
      481 ccacggtacg cctgcggcca aatttgattc ctttcagctt tgcttcctgt cggccctcat
      541 tcgtgcgttc taggatcctc cggcgttcag cctgtgccac agccgacagg atggtgacca
      601 ccatttgccc catatcaccg tcggtactga tcccgtcatc aatgaaccgg actgccacgc
      661 cctgagcgtc aaattccttt atcagttgga tcatatcggc agtgtcgcgg ccaagacggt
      721 cgagcttctt aaccagaatg acatcacctt cctccacctt catcctcagc aaat
//
LOCUS       DADLDT020000354          761 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000354 DADLDT020000000
VERSION     DADLDT020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.362"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            28..300
                     /locus_tag="JQH22_004470"
     CDS             28..300
                     /locus_tag="JQH22_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208515.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            300..605
                     /locus_tag="JQH22_004471"
     CDS             300..605
                     /locus_tag="JQH22_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208516.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            605..>761
                     /locus_tag="JQH22_004472"
     CDS             605..>761
                     /locus_tag="JQH22_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208517.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQAC"
BASE COUNT          247 a          166 c          182 g          166 t
ORIGIN      
        1 aaagtaaatg aaacagatgc tgaatacctg gtgcgtaaat tcgatgagct tgaagctaaa
       61 tgtgcagcac aggaaaacaa agtaatacca gtgtcaactg aactgccacc agcaaatgaa
      121 agtgttttgt tattcgatgc taacggagaa ggctggctaa ttggctggcg ttctctctgg
      181 tacacctggg gacaaaaaga aaccggagaa tggcagtgga catttcaggt cggggacctt
      241 gaaaacgtca atatcactca ctgggcagta atgccaaaag caccggaggc tggagcataa
      301 tgaccacttt taccgacaaa gaactgatta aagaaattaa agagcgtatc agcagccttg
      361 acgtgcgaga cgatattgag cgccgtgctt atgaaatcgc actcctatct ctggaagtag
      421 aaccagatga acgcgaagct tatgaattat tcatggaaaa gcgttttggt gacttagtag
      481 atcgtcggag agcaaaaaac ggcgataacg aatacatggc atgggatatg actctcggtt
      541 ggatcgtctg gcagcaacga gctggtatcc atttctcaac aatgtcacag caagaggtga
      601 aataatggag ccatacagcc tcacactcga tgaggcctgt cattttctca agatatccag
      661 accgactgcc attaactgga tacgcacagg gcgtcttcag gcaacacgca aagatcccac
      721 taagaataaa tctccttacc tcacaacacg acaagcctgc a
//
LOCUS       DADLDT020000355          758 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000355 DADLDT020000000
VERSION     DADLDT020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.363"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            202..>758
                     /locus_tag="JQH22_004473"
     CDS             202..>758
                     /locus_tag="JQH22_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFB2208518.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          263 a          146 c          140 g          209 t
ORIGIN      
        1 acatcacccc ccggatcatg gacgactggg ggcagtaatc atgccgggtc tggctggcta
       61 acaccccatt ataaggggta cgaataaagt aacgggggaa gcaatacctg gagaaagagt
      121 acaaagcaaa ctgtattaaa cttaataagt tatattgtat ttcactagag ttcttatcgt
      181 tgataagaaa ttctggttat aatgattaaa tcaacgaata tacaggtaat cggttctggt
      241 attatgcatc aaataaacaa catacactcg ttaactctat tttctttgcc tgtgagtttg
      301 agtccatctt gtaatgaata ttatttaaaa gtctggagtg aatgggaaaa gaacggtacc
      361 ccaggcgagc aacgcaatat cgccttcaat aggctaaaaa tatgtttgca aaatcaaggg
      421 gcggaattaa atctatctga gttagattta aaaacattac ctgatttacc gcctcagata
      481 acaacactgg aaataagaaa aaacctatta acatatctcc ctgatttccc accaatgctt
      541 aaggtaatac atgctcaatt taatcaactg gaaagcttac ctgccttacc cgagacgtta
      601 gaagagctta atgtgggtga taacaagata aaagaattac catatcttcc tgaaactcta
      661 actcatttac ggatccataa taaccgattg catattctgc cactattgcc accggagcta
      721 aaattactga tagtttctgg aaacagatta gacagcat
//
LOCUS       DADLDT020000356          750 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000356 DADLDT020000000
VERSION     DADLDT020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.365"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>750
                     /locus_tag="JQH22_004474"
     CDS             <1..>750
                     /locus_tag="JQH22_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFB2208519.1"
                     /translation="VHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGV
                     PQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKP
                     AVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFL
                     GHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGW
                     AMFYQFVDFKAKVFSYIDRVVFWKLAHWLAR"
BASE COUNT          197 a          179 c          212 g          162 t
ORIGIN      
        1 ccgtacatca tcgactgctg atgaaggctg tacgccgcag gatcagtgac gcacgtttca
       61 tgactctgct gtggaaaacc atcaaggcgg gacatatcga tgtcggtctc tttcgggcgg
      121 ccagtgaagg tgtgccacag ggcggtgtta tatcgccgct attatcgaac atcatgctga
      181 atgagttcga tcaatacctg catgagcgct acctgagcgg gaaagccaga aaagatcggt
      241 ggtactggaa taacagtatc caacggggcc gaagtacggc ggtcagagaa aactggcagt
      301 ggaaacccgc ggtggcgtac tgccgctatg ccgatgattt tgtcctcatc gtcaaaggca
      361 ccaaagcaca ggcggaagcc atcagggagg agtgtcgggg tgtgctcgaa ggcagtctga
      421 aactcaggct gaacatggat aagactaaaa tcacccatgt taatgacggc tttatctttc
      481 tggggcacag gatcattcgc aaacgcagtc gttatggcga gatgcgagtg gtctcaacga
      541 tcccgcagga gaaagccaga aacttcgccg catcgctgac agcactgtta tcaggcaact
      601 acagtgaaag caaagtcgat atggctgaac aactcaaccg aaaactgaaa ggctgggcca
      661 tgttctatca gttcgttgat tttaaggcca aagtcttcag ttatatcgac cgtgtcgtgt
      721 tctggaagct ggctcactgg ctggcccgca
//
LOCUS       DADLDT020000357          745 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000357 DADLDT020000000
VERSION     DADLDT020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.366"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..745
                     /locus_tag="JQH22_004475"
                     /pseudo
     CDS             1..745
                     /locus_tag="JQH22_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          172 a          203 c          224 g          146 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaatctg ttggagcagg
      481 atttttacgc cagtggcccg aaccagaagt gggcaggaga catcacgtac ttacgtacag
      541 atgaaggctg gctgtatctg gcagtggtca ttgacctatg gtcacgtgcc gttattggct
      601 ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga tgccctgcag atggcgctgt
      661 ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc cagtactgtt
      721 cagcagatta tcaggcgcaa ctgaa
//
LOCUS       DADLDT020000358          742 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000358 DADLDT020000000
VERSION     DADLDT020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.367"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            19..492
                     /locus_tag="JQH22_004476"
     CDS             19..492
                     /locus_tag="JQH22_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2208520.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          234 a          148 c          152 g          208 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcgaatc atcaagcccc tgttacctcc
      661 tgaacccgcc acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg
      721 catgttctgg gtgttatgtt cc
//
LOCUS       DADLDT020000359          692 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000359 DADLDT020000000
VERSION     DADLDT020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.368"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            36..>692
                     /gene="istA"
                     /locus_tag="JQH22_004477"
     CDS             36..>692
                     /gene="istA"
                     /locus_tag="JQH22_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFB2208521.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccggac gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DADLDT020000360          687 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000360 DADLDT020000000
VERSION     DADLDT020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.369"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..667)
                     /locus_tag="JQH22_004478"
     CDS             complement(<1..667)
                     /locus_tag="JQH22_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFB2208522.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DADLDT020000361          685 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000361 DADLDT020000000
VERSION     DADLDT020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.370"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..344
                     /locus_tag="JQH22_004479"
     CDS             <1..344
                     /locus_tag="JQH22_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFB2208523.1"
                     /translation="IEPRNASVIEVPDITTNRRITAPGYWFYRNDEFVFDYRLKAEDE
                     RDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNA
                     IEWPVSPEGSS"
     gene            368..>685
                     /locus_tag="JQH22_004480"
     CDS             368..>685
                     /locus_tag="JQH22_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFB2208524.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRF"
BASE COUNT          182 a          156 c          183 g          164 t
ORIGIN      
        1 gcatcgagcc acgcaatgcc agcgttatcg aagttccgga tattaccact aaccgacgga
       61 ttaccgcgcc gggttactgg ttttaccgca atgatgaatt tgtctttgac tacagactca
      121 aagcggaaga tgagcgtgat gcccttctgg ctcaggtcag tgcccggaca ggggaatggg
      181 aagaagacct gctgctgggg ttaatcagcg acgaagataa agaaaagctg aaagcctgtc
      241 gtatttacgc gaaatcgctg caggcgatgg atttcagcac catcactgat aaatcctcat
      301 acaacgccat tgaatggccc gtctctccgg aaggttcttc ctgatttaat ttatcgcgag
      361 aaaaacaatg tctgtagtga tatcaggtgc gctgactgat ggcgcaggta tccccatgtc
      421 cggataccat attattctga aatcccgggt aaacaccccg gaagtggtga tgaacactgt
      481 tgctgatgtg atggcaggaa acgatggtga atactgtttc catgcgcgga ctggaaaata
      541 tggtgtgtat ctgaaacagg actggcgcaa cgagtacaac gttggcgaca ttgctgtata
      601 tgaggactca aagcccggca cgctgaatga ctttctgatt gctcctgatg agggcgacct
      661 gaaaccggat gtcgtcaaac gcttt
//
LOCUS       DADLDT020000362          672 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000362 DADLDT020000000
VERSION     DADLDT020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.371"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
BASE COUNT          211 a          165 c          130 g          166 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga
      421 gtgtgttccc ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca
      481 ccagaatgat gaatcatcag tcccctgcca gaggttcact acgtaacctg accgacaatg
      541 caccattctg gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag
      601 gagatggtaa ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc
      661 gtctcaaatc tc
//
LOCUS       DADLDT020000363          671 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000363 DADLDT020000000
VERSION     DADLDT020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.372"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(183..656)
                     /locus_tag="JQH22_004481"
     CDS             complement(183..656)
                     /locus_tag="JQH22_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFB2208525.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          173 a          162 c          142 g          194 t
ORIGIN      
        1 tcctgcgaat atgtgagttt tgacctaatg agccacaaaa actcattttc gcccacgata
       61 atttttctac ttcctttctt tggaatgctc atttcaatat tcacataacg tcgctatcaa
      121 tcgcggaaaa ggcgtagtgc gcgaagcgaa cgaagctttt tgccgtcgat tgcatagctt
      181 tgttaaccct ttttccaaat ttgatagcaa tagttaatgt ttgaagtaaa gtgttgctca
      241 aaaacaactt cgaaggtatt tggaatactc gggaagaaaa catccccctc tggctcgatg
      301 tcgatcgtcg ataagtggag cgtagaggcc atgggtaatg tttctcggta aatttctccg
      361 ccaccagaca ctataacgtg accggtgaat tcagctagcc tgtccatggc ctcttcgatt
      421 gactgaaata caactacatt gtcatcattt gatgtccaac ctgagcgggt aacgaccgcg
      481 tatttcctat tggggagtgc gcccatagat tcaaacgtct tgcgacccac cagaagccac
      541 tgattgtagg tcaatgcttt aaaaagtagc tgctcccctt tcgcggacca gggtatgtct
      601 ggaccgcaac caatcacgcc gtttttcgct ttcgcagcca tcaatgatac tttcaaggtt
      661 ctcatcctgg g
//
LOCUS       DADLDT020000364          668 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000364 DADLDT020000000
VERSION     DADLDT020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.373"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>668
                     /locus_tag="JQH22_004482"
     CDS             <1..>668
                     /locus_tag="JQH22_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HFB2208526.1"
                     /translation="YARQGVELSRNTMVRRVSEMADKLRPLYIALNDYVLEAGKVHAD
                     DTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQ
                     GVLQADAYAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDI
                     EAEIRGSPAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNAL
                     NEFCR"
BASE COUNT          176 a          157 c          213 g          122 t
ORIGIN      
        1 tacgcgcgac agggcgtgga gctgagccgt aataccatgg tgcgcagggt atcagaaatg
       61 gcagacaaac tccgtcctct gtatatagcg ctgaatgact atgttctgga ggcaggaaag
      121 gtgcacgcag atgacactcc ggtgaaagta ctggccccgg ggaacggaaa gacgaaaacg
      181 ggtcgtctgt gggtatacgt cagggatgat cgtaatgcgg gttcatccct gccggcagcc
      241 gtctggttcg cgtattcggc agatcgcaaa ggagaacatc cgcagctcca cctggcaaag
      301 tatcagggcg tactgcaggc tgatgcctat gcaggttata acgtactgta cgaaacgggc
      361 cgggtgaagg aagccgggtg cctggcccac gcccgccgaa aaatccatga cgaggatgtg
      421 cgccgtccga cagaaatgac tcaggaagcg ctcagacgga tagcagagtt atacgacata
      481 gaagcggaga tacgtggcag tccggcagag gaacggcttg cagtcagaaa agccagaagc
      541 gtccagttga tgcagtcgtt gtacgactgg atacagttgc agaggaaaac gctgtcgaaa
      601 catgcggaga tggcgaaggc gttcgactat atcctgaatc actggaatgc gctgaacgag
      661 ttctgtcg
//
LOCUS       DADLDT020000365          656 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000365 DADLDT020000000
VERSION     DADLDT020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.374"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(151..648)
                     /locus_tag="JQH22_004483"
     CDS             complement(151..648)
                     /locus_tag="JQH22_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFB2208527.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          145 g          178 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DADLDT020000366          636 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000366 DADLDT020000000
VERSION     DADLDT020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.375"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            88..153
                     /locus_tag="JQH22_004484"
                     /pseudo
     CDS             88..153
                     /locus_tag="JQH22_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JQH22_004485"
     CDS             381..629
                     /locus_tag="JQH22_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFB2208528.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLDT020000367          617 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000367 DADLDT020000000
VERSION     DADLDT020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.376"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(93..617)
                     /locus_tag="JQH22_004486"
                     /pseudo
     CDS             complement(93..617)
                     /locus_tag="JQH22_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          170 a          149 c          125 g          173 t
ORIGIN      
        1 aactcttcgg gataacgctt accgctcatg ggcacctctc tttaagccat cttaaatgac
       61 tctgaggtgt ctgttaaacc cgtggcgatt caggtctgca tagcattatc aacattcgga
      121 tgtgcaacta cagctaatgc aacatcatcc cagcttgcaa tagtgctatc agcaacacca
      181 tctccgtcag tatctacggt gacaaagtta gatttaccac tataatcgct ggcattacct
      241 gtattaccga accaaccagt agtaccttca tcagtccatg aaccagaggt tacggtagaa
      301 tctttaacag tgatggtgtt attaaacact tcgctgctgt ttacgccagt gctgacagtg
      361 ttgtcgtcaa tatcttccca ctcataaccc tgagtcaacg taatacccgc aacgtgagag
      421 ttattagtga tggacagatc aacttcctga tccagagtaa tagccgtacc aatgttgtaa
      481 acgtctacaa catgagtatc agcggcatta ttgtaagtgc cgttatatgt gtagtgttcg
      541 tagttgtcgt cgatcgttga attatcaaca gtcagcgcca gacggtcatg gtagtaatca
      601 tccgcacgat cagcaca
//
LOCUS       DADLDT020000368          589 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000368 DADLDT020000000
VERSION     DADLDT020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.377"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>589
                     /locus_tag="JQH22_004487"
     rRNA            <1..>589
                     /locus_tag="JQH22_004487"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          145 a          141 c          178 g          125 t
ORIGIN      
        1 gtaaacgatg tcgacttgga ggttgtgccc ttgaggtgtg gcttccggag ctaacgcgtt
       61 aagtcgaccg cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc
      121 cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctggtct
      181 tgacatccac ggaagttttc agagatgaga atgtgccttc gggaaccgtg agacaggtgc
      241 tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa
      301 cccttatcct ttgttgccag cggtccggcc gggaactcaa aggagactgc cagtgataaa
      361 ctggaggaag gtggggatga cgtcaagtca tcatggccct tacgaccagg gctacacacg
      421 tgctacaatg gcgcatacaa agagaagcga cctcgcgaga gcaagcggac ctcataaagt
      481 gcgtcgtagt ccggattgga gtctgcaact cgactccatg aagtcggaat cgctagtaat
      541 cgtggatcag aatgccacgg tgaatacgtt cccgggcctt gtacacacc
//
LOCUS       DADLDT020000369          566 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000369 DADLDT020000000
VERSION     DADLDT020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.378"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..297
                     /locus_tag="JQH22_004488"
     CDS             <1..297
                     /locus_tag="JQH22_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HFB2208529.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JQH22_004489"
     CDS             complement(405..>566)
                     /locus_tag="JQH22_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208530.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLDT020000370          557 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000370 DADLDT020000000
VERSION     DADLDT020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.379"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
BASE COUNT          186 a           81 c          107 g          183 t
ORIGIN      
        1 taaaatcatt aaaatcatta acttatatga atatttcaga gttggagttc gaatgttgac
       61 acctgagtat ttcctgctaa aaatagccct gctttacgga tggttgtatt gcaataacac
      121 gaaagtagta agagattaaa ggtatctgtc agcgataact ctgcaagtga tgacattttg
      181 taaaccaaat aatgtaaatc agcaatgtat cttttcactt aattataaca aatgtggtat
      241 tcactaagaa tctgatacga gcttacatca cgactatttt agtctcatcg cgggatacag
      301 ggatgcaccg atggatctca gcacaccgta atgcaatacc tgaacaaata gaacagtaaa
      361 gtggttcagt attaatgaaa gctcttattt tccgttggga aaacttaaaa tagatgagaa
      421 ggctttttga tttcttatgt gatctcattt cgagtattga gaaatttgtg atttttaaat
      481 ttttcaataa gccctgtaag ggattggggt tttatgagaa ataaagagac gtattgtctt
      541 tgtttaaacg gtacgta
//
LOCUS       DADLDT020000371          551 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000371 DADLDT020000000
VERSION     DADLDT020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.380"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..551
                     /locus_tag="JQH22_004490"
                     /pseudo
     CDS             1..551
                     /locus_tag="JQH22_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          148 a          131 c          152 g          120 t
ORIGIN      
        1 ccgaacgata tggggcatgg aaaactgttt ataaccgctt taaccgatgg tcaaagtcag
       61 gcgtgattaa tattattttc aacaggttgc tttcgctact tgatgcaaac ggctttattg
      121 actggtctgc caccgcgctg gatggcagta atatccgggc gctgaaatgt gccgccggtg
      181 ctcaaaaaaa catcccgata tcgacggaga taatgggccg ggtcgctctc gcggcggttt
      241 tggcaccaaa atccatctgg caacagacgg aagtggcctc ccgttaaata tcgtgctaag
      301 ccccggacaa gctcatgaaa gccagttcgc gcaacgactt ctggacggga ttggcgttca
      361 gcgtcagaac ggcagcatga aacgccgtgg acatgcggta ctggctgata aagcgtattc
      421 agggcgcgca ttgcgcaacg agctgaaaaa taacggtata aaggcagtaa tcccccgaaa
      481 gtcaaatgag aaaatggcat cggatggacg tgcacagctt gatcgtgatg cgtactgcaa
      541 ccgtaatgtc g
//
LOCUS       DADLDT020000372          545 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000372 DADLDT020000000
VERSION     DADLDT020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.381"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="JQH22_004491"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="JQH22_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFB2208531.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..>545)
                     /gene="csgA"
                     /locus_tag="JQH22_004492"
     CDS             complement(190..>545)
                     /gene="csgA"
                     /locus_tag="JQH22_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HFB2208532.1"
                     /translation="LSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNG
                     ADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVN
                     VTQVGFGNNATAHQY"
BASE COUNT          145 a          124 c          130 g          146 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggt
//
LOCUS       DADLDT020000373          537 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000373 DADLDT020000000
VERSION     DADLDT020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.382"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>537
                     /locus_tag="JQH22_004493"
     CDS             <1..>537
                     /locus_tag="JQH22_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208533.1"
                     /translation="QEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICEN
                     VVRTINARMVDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSEVSDYG
                     IIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGC
                     FRHDGENITVVLSRDGDSQ"
BASE COUNT          120 a          141 c          144 g          132 t
ORIGIN      
        1 caggagattg tcgtcgaacc gattgaacag gctattctca tctctgagca gactaagctg
       61 tgggacgcca agtcatccac cggattcact cagggcacac ttcagcagga cgctatgaac
      121 atctgtgaga atgtggtccg taccatcaac gctcgcatgg tcgacgttct ggaatcctct
      181 aagctgctga agaccgtaga gcttcctgtc ctgactggta gcctgaccgc taaggcagac
      241 gctatcatgg acgctctgta tgagaacacc gagccttctt tcggttctga ggtgtctgac
      301 tacggtatta tcgcccatga gagccaactg aaagcactgt cccgtctggc tgctaaacag
      361 ggcttctctg gtgaagacgc tatcgtggat atgctgggta ccgacatcgc gtattacaac
      421 ggtgaggaca aaggtgtgtt catgttggct aaacgcttca cggccctgag cttcggctgc
      481 ttccgtcacg acggtgagaa tattacggtc gtcctttcac gtgatggcga ctctcag
//
LOCUS       DADLDT020000374          535 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000374 DADLDT020000000
VERSION     DADLDT020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.383"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            100..309
                     /gene="hha"
                     /locus_tag="JQH22_004494"
     CDS             100..309
                     /gene="hha"
                     /locus_tag="JQH22_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFB2208534.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            347..>535
                     /locus_tag="JQH22_004495"
     CDS             347..>535
                     /locus_tag="JQH22_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208535.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLT"
BASE COUNT          177 a          100 c          118 g          140 t
ORIGIN      
        1 ctctggtcag atgctgatcc ggttcccccc tggatatgga gacaccgtaa ataaagcgat
       61 ggcaatctta tcattcatct gttctcagcg ggaataaaaa tggcgaaaac aaaacaggaa
      121 tggctgtatc agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa
      181 aacagagaca gtttattaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg
      241 ttagcggaat taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat
      301 gtaaggtaat aattcagaat aattaattac caggagcaga taaacaatga agctgattat
      361 ttttattctc attgttctga ttattgcagc gttacttatc cggattatcc tcaaaagtgt
      421 aaatcagcat tctcctttgc tgatgcagtt gcatgcggca ggtatacgga ccggtgatgc
      481 agagcgaata ctgtccggcg gtgaatactg gcaacgtcag aagacgctgc tgaca
//
LOCUS       DADLDT020000375          529 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000375 DADLDT020000000
VERSION     DADLDT020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.384"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..238
                     /locus_tag="JQH22_004496"
                     /pseudo
     CDS             1..238
                     /locus_tag="JQH22_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            338..>529
                     /locus_tag="JQH22_004497"
     CDS             338..>529
                     /locus_tag="JQH22_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208536.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DADLDT020000376          494 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000376 DADLDT020000000
VERSION     DADLDT020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.385"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(146..>494)
                     /locus_tag="JQH22_004498"
     CDS             complement(146..>494)
                     /locus_tag="JQH22_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HFB2208537.1"
                     /translation="EVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVNQ
                     WRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATET
                     SVKPDACDSPPSG"
BASE COUNT          107 a          155 c          130 g          102 t
ORIGIN      
        1 ccaatgcgct gtccagtcat ggaattccct gcaaaatgtc agggaagact ctatgacctt
       61 caacgagata tgtcaataaa ttcaaaattc aatcctatcc tgacgcaatt tacacatggc
      121 atctgacatc aggttagggt atgcctcaac ctgacggcgg cgaatcacaa gcgtccggtt
      181 tgacgctggt ttcggtcgca gtgctggccc gcgcctggaa gcgctgatag cactccagcc
      241 cgcagaaatg ctccacgtac tcggcccctt ccggcgtgaa ggcggcatcg agcgggattt
      301 ccttgcagca cacgcagcaa ctggtgctcg gttcattggc gttcatggtg gtgttcctcc
      361 attggttgac gaagccgacg aaggccgccg ccggcatcgg cctggcgaac aggaaaccct
      421 gcaccgtgtc gcaacccgcc tgccgcaacc acgcaaggca gtcgggtgtt tccacaccct
      481 cggctaccac ctcc
//
LOCUS       DADLDT020000377          482 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000377 DADLDT020000000
VERSION     DADLDT020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.386"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>482
                     /locus_tag="JQH22_004499"
     CDS             <1..>482
                     /locus_tag="JQH22_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2208538.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     "
BASE COUNT          107 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cg
//
LOCUS       DADLDT020000378          481 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000378 DADLDT020000000
VERSION     DADLDT020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.387"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(52..481)
                     /locus_tag="JQH22_004500"
                     /pseudo
     CDS             complement(52..481)
                     /locus_tag="JQH22_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT          100 a          122 c          124 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
      481 a
//
LOCUS       DADLDT020000379          453 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000379 DADLDT020000000
VERSION     DADLDT020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.388"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..373)
                     /locus_tag="JQH22_004501"
     CDS             complement(<1..373)
                     /locus_tag="JQH22_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208539.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          137 a          106 c           98 g          112 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcggtatga agt
//
LOCUS       DADLDT020000380          446 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000380 DADLDT020000000
VERSION     DADLDT020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.389"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..395)
                     /locus_tag="JQH22_004502"
     CDS             complement(<1..395)
                     /locus_tag="JQH22_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208540.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DADLDT020000381          444 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000381 DADLDT020000000
VERSION     DADLDT020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.390"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..>444)
                     /locus_tag="JQH22_004503"
     CDS             complement(<1..>444)
                     /locus_tag="JQH22_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFB2208541.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          101 a          113 c           86 g          144 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaa
//
LOCUS       DADLDT020000382          439 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000382 DADLDT020000000
VERSION     DADLDT020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.391"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..202)
                     /locus_tag="JQH22_004504"
     CDS             complement(<1..202)
                     /locus_tag="JQH22_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFB2208542.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
     gene            302..>439
                     /locus_tag="JQH22_004505"
     CDS             302..>439
                     /locus_tag="JQH22_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFB2208543.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          116 a          104 c           92 g          127 t
ORIGIN      
        1 gagaataacg tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt
       61 tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct
      121 gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca
      181 aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac
      241 gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact
      301 aatgcggatg aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt
      361 tcgtgcagca ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac
      421 actggaacaa cgtcttaaa
//
LOCUS       DADLDT020000383          426 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000383 DADLDT020000000
VERSION     DADLDT020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.392"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..426
                     /locus_tag="JQH22_004506"
                     /pseudo
     CDS             1..426
                     /locus_tag="JQH22_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DADLDT020000384          418 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000384 DADLDT020000000
VERSION     DADLDT020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.393"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="JQH22_004507"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="JQH22_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFB2208544.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a          100 c          105 g           92 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtggatc
//
LOCUS       DADLDT020000385          416 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000385 DADLDT020000000
VERSION     DADLDT020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.394"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            43..>416
                     /locus_tag="JQH22_004508"
     CDS             43..>416
                     /locus_tag="JQH22_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFB2208545.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DADLDT020000386          407 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000386 DADLDT020000000
VERSION     DADLDT020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.395"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>407
                     /locus_tag="JQH22_004509"
     CDS             <1..>407
                     /locus_tag="JQH22_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208546.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLI"
BASE COUNT           85 a          144 c          102 g           76 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatc
//
LOCUS       DADLDT020000387          398 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000387 DADLDT020000000
VERSION     DADLDT020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.396"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..167)
                     /locus_tag="JQH22_004510"
     CDS             complement(<1..167)
                     /locus_tag="JQH22_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HFB2208547.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSS"
     gene            complement(167..>398)
                     /locus_tag="JQH22_004511"
     CDS             complement(167..>398)
                     /locus_tag="JQH22_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFB2208548.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT           99 a          118 c           91 g           90 t
ORIGIN      
        1 gagctacagg caatcgctgc ctgcatatcg tgtggcttta tcgctgttcc gcacgttccc
       61 gccagtcggt aatgcgccag cacagacgtt tccggatcgt gattgcagta gccaggaatt
      121 ctgacggtgc acatctgccc ccgcgccgct ttaagtaaat ccaccattac gcaaactcca
      181 gcagctgcgc ggccacattt tcgacttgtt ccggagagga aaatttacgg aacagaatcc
      241 agttccacag cacattcagt acagatttat aaacctgctg aaactcggtt tcgtccatat
      301 tcgcaaacgc gatagatttc gcccgacacc cacggctacc gtccggataa aaatgctcgg
      361 tgtaaaatcc ggcctgaatg gctacccact cgcggaaa
//
LOCUS       DADLDT020000388          375 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000388 DADLDT020000000
VERSION     DADLDT020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.397"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>375
                     /locus_tag="JQH22_004512"
     CDS             <1..>375
                     /locus_tag="JQH22_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFB2208549.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLDT020000389          372 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000389 DADLDT020000000
VERSION     DADLDT020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.398"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..311)
                     /locus_tag="JQH22_004513"
     CDS             complement(<1..311)
                     /locus_tag="JQH22_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208550.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DADLDT020000390          353 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000390 DADLDT020000000
VERSION     DADLDT020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.399"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            1..336
                     /locus_tag="JQH22_004514"
                     /pseudo
     CDS             1..336
                     /locus_tag="JQH22_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          127 a           75 c           81 g           70 t
ORIGIN      
        1 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
       61 cgcacttgat gaaattaact tgatgaaatt atcgagttgc tcaacaagca ccatgggggc
      121 accagtaacg acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc
      181 tcatgggaaa gaatcaaagg tatggactgg ccgacaatca aagctggtcg taatgcacta
      241 tggattgaac tggaaggcgt tgaatatgca ttcccggatc cacaaacaca aaacgaaacg
      301 caacaagcag gatacgatga gaatattccg gtgtaaaacc aagtgcattt tgg
//
LOCUS       DADLDT020000391          352 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000391 DADLDT020000000
VERSION     DADLDT020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.400"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(43..330)
                     /locus_tag="JQH22_004515"
     CDS             complement(43..330)
                     /locus_tag="JQH22_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208551.1"
                     /translation="MDEEYRKDLQLWFGLTHTSFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           94 a           88 c           70 g          100 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgta tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtctttacc tttgttgcaa tg
//
LOCUS       DADLDT020000392          344 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000392 DADLDT020000000
VERSION     DADLDT020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.401"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="JQH22_004516"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="JQH22_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFB2208552.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DADLDT020000393          339 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000393 DADLDT020000000
VERSION     DADLDT020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.402"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="JQH22_004517"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="JQH22_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFB2208553.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>339
                     /locus_tag="JQH22_004518"
     CDS             172..>339
                     /locus_tag="JQH22_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFB2208554.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           63 c           82 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttcc
//
LOCUS       DADLDT020000394          317 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000394 DADLDT020000000
VERSION     DADLDT020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.403"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..269
                     /locus_tag="JQH22_004519"
     CDS             <1..269
                     /locus_tag="JQH22_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFB2208555.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
BASE COUNT           82 a           86 c           88 g           61 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacag
//
LOCUS       DADLDT020000395          312 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000395 DADLDT020000000
VERSION     DADLDT020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.404"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>312
                     /locus_tag="JQH22_004520"
     CDS             <1..>312
                     /locus_tag="JQH22_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFB2208556.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TG"
BASE COUNT           90 a           76 c           66 g           80 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gc
//
LOCUS       DADLDT020000396          309 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000396 DADLDT020000000
VERSION     DADLDT020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.405"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>309
                     /locus_tag="JQH22_004521"
     CDS             <1..>309
                     /locus_tag="JQH22_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFB2208557.1"
                     /translation="GGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQS
                     WLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS
                     "
BASE COUNT           89 a           78 c           79 g           63 t
ORIGIN      
        1 tgggcggcaa cagcaaaagt tcggcgctgt tcatcagcct gctgaagcgg cttaaagcga
       61 cataccgtcg tgcgaaaacc atcacgctga tcgtggacaa ctacattatc cacaaaagcc
      121 gggaaacaca gagctggctg aaggagaacc cgaagttcag gggcatttat cagccggttt
      181 actcgccatg ggtgaatcat gttgaacggc tatggcaggc acttcacgac acaataacgc
      241 gtaatcatca gtgccgctca atgtggcaac tgttgaaaaa agttcgccat tttatggaaa
      301 ccgtcagcc
//
LOCUS       DADLDT020000397          294 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000397 DADLDT020000000
VERSION     DADLDT020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.406"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(<1..>294)
                     /locus_tag="JQH22_004522"
     rRNA            complement(<1..>294)
                     /locus_tag="JQH22_004522"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           53 a          102 c           64 g           75 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttct
//
LOCUS       DADLDT020000398          293 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000398 DADLDT020000000
VERSION     DADLDT020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.denovo.407"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..>293
                     /locus_tag="JQH22_004523"
     CDS             <1..>293
                     /locus_tag="JQH22_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFB2208558.1"
                     /translation="ELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLFG
                     QSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSEN"
BASE COUNT           89 a           71 c           77 g           56 t
ORIGIN      
        1 tgagctgctt aaagccatgt tgcgtaagca acagagtcgg cttcgacagt atgcctgtca
       61 ggtcgcgggc tatgagcagg aaattgaacg gctgaaagcg caactcgaca ggttgcgtcg
      121 tatgttgttc ggccagagtt cagagaaaaa gcgtcataag cttgaaaatc agatccgaca
      181 ggcagaaaaa cgactgtcgg aactggaaaa ccggctgaac acagccagaa atcttctgga
      241 agatgcatcg tcagtcacag attcacctga caccagtccc ccgtcagaaa acc
//
LOCUS       DADLDT020000399        12105 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.117, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000399 DADLDT020000000
VERSION     DADLDT020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.117"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(62..1774)
                     /locus_tag="JQH22_004524"
                     /pseudo
     CDS             complement(62..1774)
                     /locus_tag="JQH22_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1746..2531
                     /gene="lafU"
                     /locus_tag="JQH22_004525"
     CDS             1746..2531
                     /gene="lafU"
                     /locus_tag="JQH22_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFB2208559.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2602..3657
                     /gene="dinB"
                     /locus_tag="JQH22_004526"
     CDS             2602..3657
                     /gene="dinB"
                     /locus_tag="JQH22_004526"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFB2208560.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3709..4002
                     /gene="yafN"
                     /locus_tag="JQH22_004527"
     CDS             3709..4002
                     /gene="yafN"
                     /locus_tag="JQH22_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFB2208561.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            4005..4403
                     /gene="yafO"
                     /locus_tag="JQH22_004528"
     CDS             4005..4403
                     /gene="yafO"
                     /locus_tag="JQH22_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFB2208562.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4413..4865
                     /locus_tag="JQH22_004529"
     CDS             4413..4865
                     /locus_tag="JQH22_004529"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFB2208563.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            5111..6154
                     /locus_tag="JQH22_004530"
     CDS             5111..6154
                     /locus_tag="JQH22_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFB2208564.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            6216..6830
                     /locus_tag="JQH22_004531"
                     /pseudo
     CDS             6216..6830
                     /locus_tag="JQH22_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(6887..8344)
                     /gene="pepD"
                     /locus_tag="JQH22_004532"
     CDS             complement(6887..8344)
                     /gene="pepD"
                     /locus_tag="JQH22_004532"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFB2208565.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            8606..9064
                     /gene="gpt"
                     /locus_tag="JQH22_004533"
     CDS             8606..9064
                     /gene="gpt"
                     /locus_tag="JQH22_004533"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFB2208566.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9156..10400
                     /gene="frsA"
                     /locus_tag="JQH22_004534"
     CDS             9156..10400
                     /gene="frsA"
                     /locus_tag="JQH22_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFB2208567.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            10458..10859
                     /gene="crl"
                     /locus_tag="JQH22_004535"
     CDS             10458..10859
                     /gene="crl"
                     /locus_tag="JQH22_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFB2208568.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(10898..11953)
                     /gene="phoE"
                     /locus_tag="JQH22_004536"
     CDS             complement(10898..11953)
                     /gene="phoE"
                     /locus_tag="JQH22_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFB2208569.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         2943 a         3025 c         3136 g         3001 t
ORIGIN      
        1 ttttgtaggc cggataaggc gtttacgccg catccggcaa ttgtgcaccg atgccggatt
       61 cttacatcaa cgcccccata atcttcaact ccagctcatc cggcacttcg ttatacgaca
      121 gcacatgcag ccccggcgca aacaaccttg cataacgcgc cagcaaaggg cgtagctgcg
      181 gcggcaccag cagcaccgga tctttccccg ccgctttcat ctgctctttc acctgtggca
      241 tcgtgctctg gaactggttg agcatattcg ggtcaaccgg cacgctgtcg agcatcactt
      301 tgccggcctg ttgcgcctga ttcactacgt tggtcagcag attttccagc tcattattca
      361 gcgtatacac cgtcagctcc tgcttgcgaa cgaacggatg agtaatgctg cgccgcagcg
      421 ccaggcgcac atcggccgcc agcagaatat gatctttagt caccgtgcta ctggcgacca
      481 gcacggtggc gatggtgacg atatcgcgca gggaaacgcc ttcggtcagc agcgcccggt
      541 acactttcag caattggctg taattgagcg ccgcgcttaa atcctccgcc aggcgcggtg
      601 ccatcgacga caaacggtta tgcaactgcg tgatgtcatc atagttaaac aaatcaggaa
      661 tatagctgcg cacaatcttg ttcacatggg tggcaatcac actggcgctg tcgatcacct
      721 gataccccat attcagagcc ttcgctttct gcgcaggctg gatccaggtg accggtatcc
      781 cgtacgccgg gtcgttcccc agcacgccgt caatctcgcc gtaggtttcg ctggagggca
      841 gcgccatcag tttatccgcc ggaatatccg cttcatcagc ctcaatgccg ttgatgaaaa
      901 tagcgtactg gctgggcttg aggcggaagt tttcccgaat gcggatctcc ggcagcagca
      961 cgccgttacc gtcggaaatc acctgccgaa cgccgcgaat ccgctgggtg agcgggttgc
     1021 cctgcgcttt gtccaccagc gccaccagct tgtagccgag gcttaagctg atgggctcga
     1081 tcagcggaat agtttcccaa ctgacctgtt gttcgctggt ttcagtgatg gtgcgggtca
     1141 gcgtttcgag gcttttctct tccgcctccg ccgcctgcgg gcgtttgctc atccgccagc
     1201 cggtaaagcc aagcagggcg ctgaacagca aaaacggcag atgcggcatc cccggcacca
     1261 ccgccagcac gaacatgatc ccggtggcgg tgtagagcac cgacgggctt gccagcaact
     1321 ggtggcgcac gtcatgggcg atatcgccgt tgtcgctgac gcgggtgacg ataatcgccg
     1381 ccgcggtgga gagcagcagg gaagggatct gcgccaccag gccgtcgccg atggtcatca
     1441 gcacgtattg ctggaaggcg gcgtcggcgc tcaggttgta tttgaagatg ccgatacaga
     1501 cgccgccgct caggttgatc gccagaatca tcatcccggc gatggcgtct ccgcgtacaa
     1561 acttcgatgc cccgtccatc gcgccgtaga aatcggcctc gctggcaaca tctttacgcc
     1621 gggtttgcgc ctgcgcctgg ttgatcaatc cggcgttaag atcggcgtca atcgccatct
     1681 gtttgccggg catcgcgtcc agggtaaagc gggcagaaac ctcggaaata cgctcggcct
     1741 ctttggtgac gacgataaag ttgattgtca acagcgtcag taaatccgag cgtgaaagca
     1801 ttatcgccgc gctgcacggg cagtcgattt ttaacggtgg tgggctgtcg ccgttaaata
     1861 aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa gagacggaga
     1921 aaaaagcgcg tgatgtgaat gagaaaacgg cgctgctgaa gaagaaatcc gccaccgagc
     1981 ttggcgagtt ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg gaagcgaatc
     2041 tggagatgga gattgttccc cagggattac gcgtgctgat taaagacgac cagaatcgca
     2101 atatgttcga acgcggcagc gcgcagatta tgccgttctt taaaactttg ctggtggagc
     2161 tcgcgccagt gttcgactcg ctctataaca aaattattat taccgggcat accgatgcga
     2221 tggcctacaa aaacaatatc tacaacaact ggaacctttc gggcgatcgc gcgctttcag
     2281 ctcgtcgggt gctggaagag gccggaatgc cggaagataa agtgatgcag gtaagcgcaa
     2341 tggcggacca gatgctgctg gacgcgaaga atccgcaaag cgcgggcaat cggcgtattg
     2401 agattatggt gctgaccaaa agtgcgtccg atacgctgta tcaatacttt ggtcagcatg
     2461 gggataaagt ggtgcagccg ctggtgcaaa agctggataa gcagcaggtg ctttcgcagc
     2521 gaatgcgcta aatgctgaat ctttacgcat ttctcaaaac ctgaaatcac tgtatacttt
     2581 accagtgttg agaggtgagc aatgcgtaaa atcattcatg tggatatgga ctgctttttc
     2641 gcagcggtgg agatgcgcga caatcccgct ctgcgcgata tccctattgc tattggcggc
     2701 agccgcgaac gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa atttggcgta
     2761 cgtagcgcta tgcctactgg gatggcgctc aaattatgcc cacatctcac cttgcttccg
     2821 gggcgctttg acgcctacaa agaagcctca aatcatatcc gtgaaatctt ctcgcgctac
     2881 acctcgcgca ttgaaccgtt gtcactggat gaggcttatc tcgatgtcac cgatagcgtc
     2941 cattgccacg gttctgcgac cctcatcgcc caggaaatcc gccagacgat cttcaacgag
     3001 ctgcaactga cggcatctgc gggcgtggca ccagtaaagt ttctcgccaa aatcgcctcc
     3061 gacatgaata aacccaacgg tcagtttgtg attacgccgg cagaagttcc ggcattttta
     3121 caaaccttac cactggaaaa aatccccggc gtcggcaaag tctcggcggc aaaactggaa
     3181 gcgatggggc tgcggacctg cggtgatgta caaaagtgtg atctggtgat tctgcttaaa
     3241 cgctttggca aatttggccg cattttgtgg gagcgtagtc aggggattga cgagcgcgac
     3301 gttaacagcg aacggttacg aaaatccgtc ggcgtggaac gcacgatggc ggaagatatt
     3361 catcactggt ctgaatgtga agcgattatc gagcggctgt atccggaact tgaacgccgt
     3421 ctggcaaagg taaaacctga tttactgatt gctcgccagg gggtgaaatt aaagttcgac
     3481 gattttcagc agaccaccca ggagcacgtc tggccgcggc tgaataaagc tgacttaatc
     3541 gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg tctggtgggg
     3601 ctgcatgtga cgttgcttga cccgcaaatg gaaagacaac tggtgctggg attatgatgt
     3661 atactattat gtatattctg gtgtgcatta ttatgagggt atcactgtat gcatcgaatt
     3721 ctcgctgaaa aatcggtcaa tatcactgag ttacgtaaaa acccagctaa atactttatt
     3781 gatcaaccag ttgcggttct ttctaataat cgccccgcag gatatctctt aagtgccagc
     3841 gcattcgaag cgttaatgga catgcttgct gaacaagagg agaaaaagcc cataaaggcg
     3901 cgctttcgtc caagtgctgc aagattagag gaaattacac gccgcgctaa acaatatctt
     3961 aatgatatga cggatgatga tttcaatgac tttaaggaat aaggatgcgg gtattcaaaa
     4021 caaaacttat tcgcctgcaa cttacagcag aggaacttga tgcgttaacg gcggatttta
     4081 tttcctataa gcgtgacggt gttttgccag atatatttgg tcgcgatgca ctctacgacg
     4141 actcgtttac ctggccatta atcaaatttg agcgagttgc tcatattcat ctggcaaatg
     4201 tgaataatcc atttccgcca cagttgcgcc aattcagcag aacgaatgac gaagcgcatt
     4261 tggtatattg tcagggtgcg tttgatgagc aagcatggtt gctcattgcc attctgaagc
     4321 ctgaacctca taaactggct cgagataaca accaaatgca taaaattgga aaaatggcag
     4381 aagcgtttcg catgcgtttt tgaatttata ttatgaataa catacaaata agagattatc
     4441 atcctggcga ttttcagcaa ctatgcgcta ttttcattag agcggttacg atgaccgtca
     4501 gtcagcatta ttcactacaa caaattgccg cctgggcgca gattgacgaa tctcgctgga
     4561 aggagaaact cgcgaaatca caagtgcggg ttgcagttat taatgcaaaa ctggtcggct
     4621 ttattacctg cgttgaacac tatatcgata tgttatttgt tgaccctgaa tacacccgcc
     4681 gtggggttgc cagcgctttg ttaaaacctt ttattaagtc tgaatccgaa cttacggtgg
     4741 acgcaagcat aaccgcaaaa cccttttttg aacgttatgg ttttcagaca gttaagcagc
     4801 agcgcgttga atgccgggga gcgtggttta ttaattttta tatgcgttat aaaccgcaac
     4861 attaaatcca gcttgcaatg aaaataacgc ccgcctagta tgtgcgcgtt ttcttattcc
     4921 acaaactgca aggaggtaaa tcatgacaaa tcctttatca atgactcttt gcagaccttt
     4981 ccaggattaa ttcttttttt cttgccctgg attcgtctgc catttcctga ttttttaatt
     5041 gttactctca ataattcaag tcgcagcaca tgcaacttga agtatgacga gtctatttac
     5101 ggaatggtat atgggcaatt atattcgtcc cttatccgat gcggtattta ccatcgcatc
     5161 tgatgacctg tggatcgaga gtttagcgat ccaacaatta cacaccacgg caaatttacc
     5221 caacatgcag cgcgtagtgg ggatgccaga tttacacccc ggacgcggct acccgattgg
     5281 cgcagcgttc ttctccgcag gccgttttta cccagcactg gtcggcaatg atatcggctg
     5341 tggcatggcg ctatggcaaa cagatattct cgctcgcaaa tacaacgccg ataagtttga
     5401 aaagcgatta tcagctttgg atgacgttgc tgaagaaagc tggctggagg aaaacctgcc
     5461 gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc tctatcggtg gtggcaatca
     5521 tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct gaactgtttg cactggcagg
     5581 tctggatgcg cagcatctgc aactgctggt tcatagcggc tcgagggggc tagggcagtc
     5641 tattttacag cggcatattg cctcgttttc gcatcatggt ttgcctgaag gcagtgacga
     5701 cgcgctggcg tttgcgcgtt ttaatcgcca cctgatcgct ttgcgcataa tgcaacaggt
     5761 taaggccacc ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcgaat
     5821 cggtgatcaa caaggggtct tacatcgtaa aggtgccacg cctgatgact gtggtctggt
     5881 ggttattccc ggttcacgcg gtgattactc caggctggtt cagcccgtca ggagtgagga
     5941 aacattgcat tcgctggcgc atggggctgg gcgtaaatgg gggcgcaccg agtgtaaagg
     6001 gcgtctggca gcgaaataca cagcgacgca actctcgcgt actgaacttg gcagtcgggt
     6061 aatttgtcgc gataaacaac tcatcttcga agaagcgcca caagcctata aatcggctga
     6121 aagtgtggtg caatgtctgg gcaggccgga ttaattattc ctgtcgcgcg gctgcgtccg
     6181 gtgctaacgc tcaaaaacag tggagggaaa aaaggatgat cttgctataa ctctcctcag
     6241 cgcaggggcc ggaagaatgt tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag
     6301 aagctgcccg acaagacgtc gcggtaacgg tgctggaaac agaaacaggc cgctactctg
     6361 acacgctgcg ttcggcgctg atttctctgg atggcgacaa cgcatgggcg ttaagcgaaa
     6421 gctggtgtgg cactattcag tggatttgcc cgagcccgta tcgcccacat cacgggcgta
     6481 aaaactggtt tctgggcatt gggcgtttta ccgctgatga gcaggagcaa tccgatgcga
     6541 tccggtatga gacgctgcgt tcgtcggggc cgggcggtca acatgtcaat aaaaccgact
     6601 cggcggtacg cgccacgcat ttggcatccg gtattagcgt gaaggttcag tcagagcgca
     6661 gccagcatgc taacaaacgg ctggcgcgat tgctgattgc ctggaagctg gatcagcagc
     6721 aataggaaaa tagcgcggtg ctgaaatcac agcggcgaat gttccatcac cagattgaac
     6781 gtggcaaccc gcgacgaacg tttacaggga tggcttttat cgaaggataa tgaaggatga
     6841 aactgccgga aggcgattaa acgccatccg gcagcaaatc aaataattac ttcgccggaa
     6901 tttctttcag cagttcagtc agcagtgtcc agtaatgacc tacgctttcg atgtgaactt
     6961 gctcatccgg agagtgtgga ccggtgatag ttggcccgat agaaaccatg tccatttccg
     7021 gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggcg
     7081 tcttgttgaa caggcgctga taggtttcac gtaccagatg catcaccgga gaattagcgt
     7141 ccggctgcca gccaggatat gcgcctttcg cttcggtttt cgcgccagcc agtttaccca
     7201 gcgaatccag catgctcacc acgtagtctt taccgctgtc gatcagtgaa cggatcaggc
     7261 agtgaatttc tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga
     7321 ccacaccttt tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac
     7381 gaataaaggt atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca
     7441 gcaacaaggc cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg
     7501 tattcaccag agatttcagt gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg
     7561 cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca
     7621 gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa
     7681 cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt
     7741 caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatccccc
     7801 ccgcacaacc catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag
     7861 cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg
     7921 tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcag
     7981 aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc
     8041 catcaatata aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct
     8101 gcggcaccat atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg
     8161 tagcaggttt acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct
     8221 cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa
     8281 tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag
     8341 acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca
     8401 gaaaatgttg tcaacacaag acaggcttgc gagatatgta ttgagaatac cactttatcc
     8461 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     8521 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     8581 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     8641 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     8701 tattgccgta agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg aactgggtat
     8761 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     8821 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     8881 taccggtggt actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     8941 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9001 tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9061 ctaatctttt caacgcctgg cattgccggg cgttgttctt tttaacttca ggcaggttac
     9121 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     9181 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     9241 acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc
     9301 gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa atcctcgacg
     9361 tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta tacgataccg
     9421 tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc
     9481 aacaaaaagc ctgtacggaa gaagatccga aactcagtgg tcgacactgg ctgcatgcgg
     9541 ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag
     9601 cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc
     9661 ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga
     9721 aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg
     9781 actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg
     9841 atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc
     9901 atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg
     9961 cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc
    10021 gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt
    10081 gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg
    10141 atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat
    10201 tgctggggcg tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca
    10261 gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga
    10321 tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga
    10381 tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat
    10441 cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa
    10501 aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt
    10561 tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg
    10621 gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac cagtttggtc
    10681 tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agatactgaa gtggttgaac
    10741 gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga
    10801 atctgaagct ggaaccggcg aatgattttc gtgatgaacc ggtgaagtta acggcgtgag
    10861 tgaaatgtgc cggatgcatc acatccggca atattcatta aaactgatac gtcatcccaa
    10921 ccgcgacagt atcatcatta ttaatattca gcttattatc gctatccagt tggttgattt
    10981 tataatcaac aaacgctgac atatttttgt tgaaataata cgtagcaccg acgtcgatat
    11041 aattgaccag atcttcatca ccgatgccct caatatcttt ccctttcgat aagacataac
    11101 ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg
    11161 tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa tagaaagttg
    11221 ccagataaat attattggca tcgtatttca gacctgttgc ccatgcttct gcacgcttgc
    11281 ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggccccac
    11341 taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt
    11401 tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc
    11461 catcgataat gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg
    11521 tcataaagtt gtcggtctgc gccgaggaat cgccaccaaa ttccgggaac atatcggtcc
    11581 aggcttccac gtcatacaac gcgcccaggt tacgaccata atcgaaagaa cccaaatctt
    11641 tatatttcaa cccggcaaaa gcgagacgcg ttttttgctg tgcagtatca ctctccgctt
    11701 tatttccggc aaactccgct tcccaccgac cataaccagt cagttgatcg ttaatttgag
    11761 tttcaccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg gcgttatcac
    11821 tcatataatg catggcttta actttgccat agacatccag tttattaccg tctttattat
    11881 atatttctgc agcctgtaca gatgcagatg ccacaatgcc catcaccact aatgccagag
    11941 tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc
    12001 ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac agattaatga
    12061 tattctgaaa atgggtaatg ctgccaactt actgatttag tgtat
//
LOCUS       DADLDT020000400         9721 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.138, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000400 DADLDT020000000
VERSION     DADLDT020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.138"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(16..204)
                     /locus_tag="JQH22_004537"
     CDS             complement(16..204)
                     /locus_tag="JQH22_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFB2208570.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JQH22_004538"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JQH22_004538"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFB2208571.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="JQH22_004539"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="JQH22_004539"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFB2208572.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="JQH22_004540"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="JQH22_004540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFB2208573.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="JQH22_004541"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="JQH22_004541"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFB2208574.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="JQH22_004542"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="JQH22_004542"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFB2208575.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6232
                     /locus_tag="JQH22_004543"
     CDS             5099..6232
                     /locus_tag="JQH22_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFB2208576.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="JQH22_004544"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="JQH22_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFB2208577.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="JQH22_004545"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="JQH22_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFB2208578.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="JQH22_004546"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="JQH22_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFB2208579.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="JQH22_004547"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="JQH22_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFB2208580.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="JQH22_004548"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="JQH22_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFB2208581.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="JQH22_004549"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="JQH22_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFB2208582.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2476 a         2486 c         2472 g         2287 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accaactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatcc
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca acctgtcatt aaactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt cttttcggta atgactccaa cttactgata gtgttttatg
     9721 t
//
LOCUS       DADLDT020000401         9161 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.145, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000401 DADLDT020000000
VERSION     DADLDT020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.145"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            109..1014
                     /locus_tag="JQH22_004550"
     CDS             109..1014
                     /locus_tag="JQH22_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFB2208583.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="JQH22_004551"
     CDS             1011..2249
                     /locus_tag="JQH22_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFB2208584.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..2833
                     /locus_tag="JQH22_004552"
     CDS             2249..2833
                     /locus_tag="JQH22_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFB2208585.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3326..4090)
                     /locus_tag="JQH22_004553"
     CDS             complement(3326..4090)
                     /locus_tag="JQH22_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFB2208586.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4208..4273)
                     /locus_tag="JQH22_004554"
                     /pseudo
     CDS             complement(4208..4273)
                     /locus_tag="JQH22_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4317..4622)
                     /locus_tag="JQH22_004555"
     CDS             complement(4317..4622)
                     /locus_tag="JQH22_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFB2208587.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4633..5838)
                     /gene="chrA"
                     /locus_tag="JQH22_004556"
     CDS             complement(4633..5838)
                     /gene="chrA"
                     /locus_tag="JQH22_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFB2208588.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5994..6197)
                     /locus_tag="JQH22_004557"
     CDS             complement(5994..6197)
                     /locus_tag="JQH22_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFB2208589.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6325..7164)
                     /gene="sul1"
                     /locus_tag="JQH22_004558"
     CDS             complement(6325..7164)
                     /gene="sul1"
                     /locus_tag="JQH22_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFB2208590.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7158..7505)
                     /locus_tag="JQH22_004559"
     CDS             complement(7158..7505)
                     /locus_tag="JQH22_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFB2208591.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7711..8499)
                     /locus_tag="JQH22_004560"
     CDS             complement(7711..8499)
                     /locus_tag="JQH22_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFB2208592.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8630..9103)
                     /locus_tag="JQH22_004561"
     CDS             complement(8630..9103)
                     /locus_tag="JQH22_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFB2208593.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT         1933 a         2707 c         2626 g         1895 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtggtgg aggggatccg
     2641 caagcgactg cccccaggtg ctcctgcggc agctgagttg ctcctgcact cggtcatcgc
     2701 tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag ctagctgatc atgtgctggc
     2761 tcagatcgct gccatcctgt gtttaatgtt tcccgaacac gacgatttcc aactcctcca
     2821 ggcacatgcg taaacggagg tgtgcagagt ccctgcggca ggcgacgaac acgaccgtcg
     2881 tcgattagta ccggtacggt cggtggtatc gaagtcttga tcaccactca ggtctacggc
     2941 ttacaaatgg tgaccatccc gatacttgcg tcagagcacc gggccgattc tttgacagtg
     3001 aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg tcgagacgat actccgccaa
     3061 tgcgcccagc aaacaacctg gccatcgcag gtggtgggga gcggtgtggc ggatgagttg
     3121 gacaagttgg tgtagcagca cgagcacggc gagataacat cgcaggagtt cgacatgctc
     3181 aagagacagc tgattgcgaa tcgcgatgca gattcataac ccgattgcgg gttggcttca
     3241 ctccaccatc accgagcaga ctagcacggc gggctctgtt gcaaagattg gcggcagtca
     3301 gaggtaggct gtcgctctgc gccgatcagg cggctgctgc gaaatggtgg ttgagcatgc
     3361 ccatggcctc cgtcagcgcc gagggcccaa tgccaaaagc tctctccaca aggcgcacct
     3421 cgcccctgat gccgggctgc aggcaccagg ggcgagcctg tcctttgcgc agggctcgca
     3481 tgacttcgaa tcccttgatc gtggcatagg ccgtggggat cgatttgaaa ccgcgcaccg
     3541 gcttgatcag tatcttgagc tttccgtgat cggcctcgat cacgttattg agatacttca
     3601 cctgccggtg ggccgtctcc cggtccagct ttccttcgcg cttcaattcg gtgatcgctg
     3661 caccatagct cggcgctttg tcggtattga gcgtggcagg cttttcccag tgcttcaggc
     3721 ctcgcagggc cttgcccagg aaccgcttcg ctgccttggc gctgcgggtc ggcgacaggt
     3781 agaaatcgat cgtgtcgccc cgcttgtcga ctgcccggta caggtaggtc cacttgcccc
     3841 gcaccttgac gtaggtttca tccaggcgcc agctcggatc aaagccacgc cgccagaacc
     3901 agcgcagccg cttctccatc tccggggcgt agcactggac ccagcgatag atcgtcgtat
     3961 ggtcgaccga aatgccgcgt tccgccagca tttcctcaag gtcgcgatag ctgatcggat
     4021 agcgacaata ccagcgcacc gcccacagga tcacatcacc ctggaaatgg cgccacttga
     4081 aatccgtcat cgttccgtcc gtccaatctc cgccaagcat gctcaagctt cacgattttt
     4141 gcaacagagc ccacacgagt attgagcata gtcgagattg gtgcagatca cttctgatat
     4201 tgaactgtca ggagctggct gcacaacagc cattacgccc aatcaactgg tgcagtcgtc
     4261 ttctgaaaat gacaatccag ttagggtata gctcaacctg acatagaagc aaaaactcaa
     4321 ccaccttcta ccaactctcc gaacagctcc ttgacctttg ttttcgcatc agcaagtgca
     4381 gttctgcctt gttcagtgat gtcataaaca cgccgttcac gtcgcccggt gcgttcgtgg
     4441 cgtgaggtca gatagccttt tttttccagg ccgtgcagca tcgggtacac ggtgccagcg
     4501 ctcatctcgt agccgtgtcg gcgtagctct tcgatgatcc ccagcccaaa gacaggttcc
     4561 tcggctgcat ggtgaaggat gtgcaggcgg atcaaaccgc cgtagaggtc tttgtcagtc
     4621 attttttgtg cctcacagag cgacgctcaa cagccaccca gctgcaccgc taccgaggac
     4681 aaccagccac ggcgggagct tccagaacat aagtgcgaca agggcaacta atgccaagcc
     4741 gaagtcttgc ggctgaaaga tggcgctagt ccatacaggc tgatacagcg cggccagcag
     4801 caagccgact acagcggcat tgatcccggc cagcgcagct tggatgcctg tattgcggcg
     4861 caaacgctcc caaaatggca ttgatccgac gaccagcaag aacgagggcg cgaagatagc
     4921 cagcagacac acaatgccgc cgatccagcc cgacggggcg gtgttcatcg aggcaccaag
     4981 aaacgcggcg aacgtgaaca aagggccggg caccgcttga gctgccccgt accccgcgag
     5041 aaaggattca ttgttgaccc agccggaggg caccacttcg gcttgcagta atggcagcac
     5101 aacgtgacca ccgccgaaca ccagtgatcc gacacgatag aaggaatcca ccattgccat
     5161 ggtttgactt ggcatcagtt cggccaacac cggcaggcca atcagcaaga caaagaacag
     5221 cgagagccaa agcacgccgg cccggtgact gaccgtgata ggtagggggt catgctcaac
     5281 aactttcgct ggcttgaaca ataaccggcc tgcgatgcct gcgatagcaa tcacgccaac
     5341 ctgtccccac gcggacggca caagtaaaac gacgcaggta gcaattgcca tgatggtgac
     5401 tcgcagccca tccgtgcata ggttacgcgc catgccccat actgcttgag cgaccacggc
     5461 cacagccacc acttttaagc catgcaacgc gccctgcgag acgtaatcgc catagctgga
     5521 gatgccgagc gcaaaaagga tcaaggctat ggcagacggc agcgtgaagc cagcccaagc
     5581 agccagcgcc ccgctgtatc cagcccgaga cagtcctacc gctatgccga cctggctgct
     5641 tgcaggccct ggcaagaact gacaaagcgc gaccaagtca gcatagctcc gttcggagag
     5701 ccagcgccgc cgtgtgacaa attcggcgcg gaagtagccc aagtgcgcaa tggggccgcc
     5761 aaaagatgtc aatccaagcc gcagaaaaat aagaaagacc gaccatggtc tgctgtcatc
     5821 ggtagggtta ttcgtcatac tttcgccttc atgatctgca acgagttgat caataataag
     5881 cgaaattcga taacgaaatt cgatataaat ctagaaaaaa atacctctat gtgtactacg
     5941 cagttttagc tgtggctttc acaggagcac gcttacttac ggcttagcgt gctttatttt
     6001 ccgttttctg aggcgatccc taggagctcg gatctcagga cgaaggtctc cgcgaatgtc
     6061 cggtcgatcc gcgcgacgtc ccaggcgggc gttcccttgg cggacatcca cgccgcagcg
     6121 tcgtgcatca gccgcacaac ctcgtcgata tcacccgagc aggcgacccg aacgttcgga
     6181 ggctcctcgc tgtccattcg ctcccctggc gcggtatgaa ccgccgcctc atagtgcagt
     6241 ttgatcctga cgagcccagc atgtctgcgc ccaccttcgc ggaacctgac cagggtccgc
     6301 tagcgggcgg ccggaaggtg aatgctaggc atgatctaac cctcggtctc tggcgtcgcg
     6361 actgcgaaat ttcgcgaggg tttccgagaa ggtgattgcg cttcgcagat ctccaggcgc
     6421 gtgggtgcgg acgtagtcag cgccattgcc gatcgcgtga agttccgccg caaggctcgc
     6481 tggacccaga tcctttacag gaaggccaac ggtggcgccc aagaaggatt tccgcgacac
     6541 cgagaccaat agcggaagcc ccaacgccga cttcagcttt tgaaggttcg acagcacgtg
     6601 cagcgatgtt tccggtgcgg ggctcaagaa aaatcccatc cccggatcga ggatgagccg
     6661 gtcggcagcg accccgctcc gtcgcaaggc ggaaacccgc gcctcgaaga accgcacaat
     6721 ctcgtcgagc gcgtcttcgg gtcgaaggtg accggtgcgg gtggcgatgc catcccgctg
     6781 cgctgagtgc ataaccacca gcctgcagtc cgcctcagca atatcgggat agagcgcagg
     6841 gtcaggaaat ccttggatat cgttcaggta gcccacgccg cgcttgagcg catagcgctg
     6901 ggtttccggt tggaagctgt cgattgaaac acggtgcatc tgatcggaca gggcgtctaa
     6961 gagcggcgca atacgtctga tctcatcggc cggcgataca ggcctcgcgt ccggatggct
     7021 ggcggccggt ccgacatcca cgacgtctga tccgactcgc agcatttcga tcgccgcggt
     7081 gacagcgccg gcggggtcta gccgccggct ctcatcgaag aaggagtcct cggtgagatt
     7141 cagaatgccg aacaccgtca ccatggcgtc ggcctccgca gcgacttcca cgatggggat
     7201 cgggcgagca aaaaggcagc aattatgagc cccataccta caaagcccca cgcatcaagc
     7261 ttttgcccat gaagcaacca ggcaatggct gtaattatga cgacgccgag tcccgaccag
     7321 actgcataag caacaccgac agggatggat ttcagaacca gagaaagaaa ataaaatgcg
     7381 atgccataac cgattatgac aacggcggaa ggggcaagct tagtaaagcc ctcgctagat
     7441 tttaatgcgg atgttgcgat tacttcgcca actattgcga taacaagaaa aagccagcct
     7501 ttcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag
     7561 cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcatag
     7621 ttgagcggcg ggcgcagccc gtccgcttga acgccgagtt aggcatcaga tgccctcggc
     7681 gcgggtcgat gcacttttcg cacatgccgc tcaacgcaag attctctcaa tcgttgcttt
     7741 ggcatatcga acgaacgcgg ccgtctcttc gacgcgcatt gctaggtcgt cgtcctcgct
     7801 acccaggtac gccgcgcgtg ccttgcagat gaggggccga tgctcggcag gcaaacgctc
     7861 cgatacccat gcggcagcaa cgtccttagg agcaatgaga ccagttgaag cgctgtacca
     7921 aatgcgagca agagcaagaa cgacgttccg ctcgtcaccc ttccaatccg actctgcatt
     7981 ccactgggca atagtgtcga aaagcgcctt ggagaaatgc tccttcggca ccggctcgaa
     8041 aaacgtggct gcggatgggc ctagaagcgc aaggctgtgt tgcctcgcct tggtcagcaa
     8101 aatcgcaaga tcgtgatcca gaacggcagg ctcgaacgtt ccggaaagga tgtcgtggcg
     8161 gagccactca ccgaactgaa gctcacgccg cgccggatag cgccaaggca ctacttcgct
     8221 tcgagcgaca acagttagct ccagcggtcg ccatgttccg ccatcgcctg gcggtgatga
     8281 gactttcagc aaatcgagca ttagcgcctg ccggagcgaa tcgttaggtg cggcgctgac
     8341 ggtcacgagc aagtctatgt cgctgtccgg cttcagccct ccatcgatcg cagatccgaa
     8401 caggtggatt gtgtccagtg tcgcagccag atggcgctcg atcaccgcgc gagcgtggga
     8461 cagctgcttg aaaacttgtg cagggaaaaa ttcacccatg atgcctaacg ttaagttcag
     8521 cggcagcttt taagttgcgg ctttgtggaa tacttttgcg cagcaaaacc acaaagacgc
     8581 gacttaaaag ctgtccaagg agcgaagcga ctggtgctgc aacgcattgt tagccttttt
     8641 tccaaatctg gtatgtataa tttatattag acataaaaaa ctgttcaaaa accaaattga
     8701 aattctcagg cattataggg aatttgatat caccttcgac ttcaacgtga acagtagaca
     8761 aatgaattat atctgctttt tcaataaggc tattatagat ttgacccccg ccagagacat
     8821 atacatgatc tgtaactttt gatagctctt tcaaagcatt ttctattgaa ggaaaaacta
     8881 ggacgttttc atttgagctt gaaattccgt tctttgacac tactgcatat ttgcgatttg
     8941 gaagaacacc catagagtca aatgtttttc ttccgacaag gagccattga ttatatgtga
     9001 gcgctttaaa gagtagttgc tcacctttta ctgaccacgg gatatcagga ccactaccga
     9061 ttacgccatt ttctgacact gcagaaatca atgatatttt caatttaact cccttaatgg
     9121 ctaactttgt tttagggcga ctgccctgct gcgtaacatc g
//
LOCUS       DADLDT020000402         6700 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.168, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000402 DADLDT020000000
VERSION     DADLDT020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.168"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..1806
                     /locus_tag="JQH22_004562"
     CDS             <1..1806
                     /locus_tag="JQH22_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFB2208594.1"
                     /translation="RQKAASSLTLQQCQLRAAPAAKDVLDAIEVLRGMNSDNARKVPA
                     DAPTEFIKPRWQKLVMTDTGIDRRYYELCALSEMKNALRSGDIWVQGSRQFKDFEDYL
                     VPPAKFASLKQASELPLAVATDCNRYLNDRLTLLETQLATVNRMATANELPDAIITES
                     GLKITPLDAAVPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAK
                     DKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQF
                     RHPFAEHWGDGTTSSSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHT
                     KVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDL
                     GDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKL
                     GSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFN
                     RLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLS
                     PLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            1885..2889
                     /locus_tag="JQH22_004563"
     CDS             1885..2889
                     /locus_tag="JQH22_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HFB2208595.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(3071..3247)
                     /locus_tag="JQH22_004564"
     CDS             complement(3071..3247)
                     /locus_tag="JQH22_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HFB2208596.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            3577..4392
                     /gene="sul2"
                     /locus_tag="JQH22_004565"
     CDS             3577..4392
                     /gene="sul2"
                     /locus_tag="JQH22_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFB2208597.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4453..5256
                     /locus_tag="JQH22_004566"
                     /note="aph(3'')-Ib"
     CDS             4453..5256
                     /locus_tag="JQH22_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFB2208598.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            5256..6092
                     /locus_tag="JQH22_004567"
     CDS             5256..6092
                     /locus_tag="JQH22_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFB2208599.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6199..6603)
                     /locus_tag="JQH22_004568"
                     /pseudo
     CDS             complement(6199..6603)
                     /locus_tag="JQH22_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1455 a         1930 c         1862 g         1453 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc agctgcgggc cgctcccgcc
       61 gcgaaggacg tgctcgacgc catcgaggtg ctgcgcggca tgaacagcga caacgcccgc
      121 aaggtgcccg ccgacgcgcc gaccgagttc atcaagccgc gctggcagaa gctggtcatg
      181 accgacaccg gcatcgaccg gcgctactac gaactgtgcg cgctgtcgga gatgaagaac
      241 gcgttgcgtt ccggcgacat ctgggtgcag gggtcgcgcc agttcaagga cttcgaggac
      301 tacctggtgc cgcccgcgaa attcgccagc ctcaagcagg ccagcgaatt gccgctggcc
      361 gtggccaccg actgcaaccg gtacctgaac gaccggctga cgctgctgga aacacagctt
      421 gccaccgtca accgtatggc gacggccaac gagctgccgg acgccatcat caccgagtca
      481 ggcttgaaga tcacgccgct cgacgcggcg gtacccgaca ccgcccaagc gctgatcgac
      541 cagacggcaa tgatcctgcc gcacgtcaag atcaccgaac tgctgctgga ggtggacgaa
      601 tggacgggct tcactcggca tttcgcgcat ctgaaatcgg gcgacccggc caaagacaag
      661 aacctgttgc tgaccacgat cctcgccgac gcgatcaacc tgggcctgac caagatggcg
      721 gagtcttgcc ccggcacgac ctacgccaag ctggcttggc tgcaagcctg gcacatccgc
      781 gacgaaacct acggggcggc gctggccgat ctggtcaacg cacagttccg ccatcccttc
      841 gccgagcact ggggcgacgg caccacctca tcgtcggacg gccagaactt ccgcaccggc
      901 agcaaggccg agagcaccgg ccacatcaac ccgaaatacg ggagcagccc agggcggacg
      961 ttctacaccc acatttctga ccagtacgcg ccatttcaca ccaaggtcgt gaacgtcggc
     1021 gtgcgcgatt cgacctacgt gctcgacggc ctgctgtacc acgagtccga cttgcggatc
     1081 gaggagcatt acaccgacac ggcgggcttc accgatcacg tcttcgccct gatgcacctc
     1141 ctgggcttcc gcttcgcgcc gcgcatccgc gacctgggcg acaccaagct ctacatcccg
     1201 aagggcgacg ccgcctatga cgcgctgaaa cccatgatcg gcggcacgct caacatcaag
     1261 cacgtccgcg cccattggga cgaaatcctg cggctggcca cctcgatcaa gcagggcacg
     1321 gtgacggcct ccctgatgct ccgaaagctc ggcagctacc cacgccagaa cggcctggcc
     1381 gtggcgctcc gcgagctggg ccgcatcgag cgcacgctgt tcatcctgga ctggctgcaa
     1441 agcgtggaac tgcgccgccg cgtgcatgcc ggcctgaaca agggcgaggc gcgcaatgcg
     1501 ctggccaggg cagtgttttt caaccgcctg ggtgaaatcc gcgaccgcag tttcgagcag
     1561 cagcgctacc gggctagcgg cctcaatctg gtaacggctg ccgtcgtgtt gtggaacacg
     1621 gtctatctgg aacgggctgc gcacgcgctg cgtggcaacg gccatgccgt tgatgacgcg
     1681 ctgttgcagt acctgtcgcc gctcggttgg gagcacatca acctcaccgg cgattacctc
     1741 tggcgcagca gcgccaagat cggcgcgggc aagttcaggc cgctacgacc gctgcaaccg
     1801 gcttagcgtg ctttatttaa tgagatggtc actccctcct tcccagtact atgctgagga
     1861 caggctttca ttcggagaac catcatggaa aacattgcgc ttattggtat cgatctgggt
     1921 aagaactctt tccatattca ttgtcaggat catcgtggga aggccgttta ccgtaaaaaa
     1981 ttcacccgac caaagctaat cgaatttctg gcgacatgcc cggcaacaac catcgcgatg
     2041 gaagcctgtg gcggttctca ctttatggca cgcaagctgg aagagttagg gcattttcca
     2101 aagctgatat caccgcaatt tgtccgccca ttcgttaaaa gcaacaaaaa tgacttcgtt
     2161 gatgctgaag ctatctgtga agcagcatca cgtccatcta tgcgtttcgt gcagcccaga
     2221 accgaatctc agcaggcaat gcgagctctg catcgtgtcc gtgaatccct ggttcaggat
     2281 aaggtgaaaa caactaatca gatgcatgct tttctgctgg aatttggtat cagcgttccg
     2341 cgaggtgctg ccgttattag tcgactgagt acccttcttg aggacagtag tttgcctctt
     2401 tatctcagcc agttactgct gaaattacaa cagcattatc actatcttgt tgagcagatt
     2461 aaagatctgg aatctcagtt gaaacgaaag ttggacgaag atgaggttgg acagcgcttg
     2521 ctgagtattc cctgcgttgg aacgctgact gccagtacta tttcaactga gattggcgac
     2581 gggaagcagt acgccagcag ccgtgacttt gcggcggcaa cagggctggt accccgacag
     2641 tacagcacgg gaggtcggac gacattgtta gggattagca agcggggcaa caaaaagatc
     2701 cgaactttgt tggttcagtg tgccagggta ttcatacaaa aactggaaca ccagtctggc
     2761 aagttggccg actgggtcag ggagttgttg tgtcggaaaa gcaactttgt cgtcacctgt
     2821 gctctggcaa acaagctggc cagaatagcc tgggcactga cggcgcgaca gcaaacttac
     2881 gaagcataaa ggcagaaata caccagttta aacaatcatt catctggttt tgcgaatact
     2941 gatattgatg atactaacgg cccaccggcc tgttgaggaa cctgtaaaac ggaaaggctc
     3001 attgaagccg tatattttct ggaggttcat caggcgcgga actcatcgag gcgcgggaat
     3061 aaaatcccat tcagacgccg gatagattca agcaagccaa cttgtcgtca aaatcggtgt
     3121 tgcaaaaacg ggagtgacca tagattccgt tttctgaggc gacccccaca tagagacctt
     3181 cccggtcatt cactttgtag agtttgtccc tgggcttgag attgcgcagc ttggtatcgg
     3241 tcagcatgta ccttgtcctt cttcgtctcc gatacccact ctattgtaaa caagacattt
     3301 ttatctttta tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca
     3361 tgctcagaaa aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct
     3421 ccataggccg ctttcctggc tttgcttcca gatgtatgct attctgctcc tgcagctaat
     3481 ggagcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
     3541 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
     3601 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
     3661 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
     3721 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
     3781 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
     3841 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
     3901 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
     3961 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
     4021 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
     4081 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
     4141 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
     4201 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
     4261 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
     4321 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
     4381 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
     4441 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
     4501 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
     4561 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
     4621 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
     4681 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
     4741 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
     4801 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
     4861 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgcag
     4921 ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
     4981 gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact
     5041 cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat
     5101 ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc
     5161 gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc
     5221 ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg
     5281 ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga
     5341 aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca
     5401 ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc
     5461 ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct
     5521 ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg
     5581 cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc
     5641 gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact
     5701 acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc
     5761 tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag
     5821 atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg
     5881 ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct
     5941 ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc
     6001 tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg
     6061 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
     6121 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
     6181 cttcccgcaa acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg
     6241 acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg
     6301 ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc
     6361 ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag
     6421 aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag
     6481 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg
     6541 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg
     6601 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc
     6661 ttttaggggt ctgacgctca gtggaacgaa aactcacgtt
//
LOCUS       DADLDT020000403         5751 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.199, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000403 DADLDT020000000
VERSION     DADLDT020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.199"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            complement(45..1060)
                     /locus_tag="JQH22_004569"
                     /pseudo
     CDS             complement(45..1060)
                     /locus_tag="JQH22_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1455..2111
                     /locus_tag="JQH22_004570"
     CDS             1455..2111
                     /locus_tag="JQH22_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFB2208600.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(2430..2846)
                     /locus_tag="JQH22_004571"
     CDS             complement(2430..2846)
                     /locus_tag="JQH22_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208601.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(2891..4282)
                     /locus_tag="JQH22_004572"
     CDS             complement(2891..4282)
                     /locus_tag="JQH22_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFB2208602.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4319..4891)
                     /locus_tag="JQH22_004573"
     CDS             complement(4319..4891)
                     /locus_tag="JQH22_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208603.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(5028..5618)
                     /locus_tag="JQH22_004574"
     CDS             complement(5028..5618)
                     /locus_tag="JQH22_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFB2208604.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
BASE COUNT         1365 a         1518 c         1372 g         1496 t
ORIGIN      
        1 aacgtgagtt ttcgttccac tgagcgtcag acccctaaaa tcctgatgct gatgtagtcc
       61 cgttttatcg tcatcacgaa gctttctgct atgccattgc tttccgggct tcgcactgct
      121 gtcgtacaag gctccagtcc cagcaaccgg gcgaacgccg gcactgttgg caactgcctt
      181 ccgaagcgtt tttccaccgc acccagcatg acatcctgca ccgtttcttt gtcatagcct
      241 ccggtgctcg ctgcccagtc aatgatctcc ctgtcgcagc agtcctgcgc gaaggtcacc
      301 cgcagctttt cgccattatc acagcggaac tcaaagccat ctgagcacca gcgccggtta
      361 ctttccgcta ctgctacgcg ccccttatgg gcccgcttac ggcaaggatc agcgggttta
      421 cgttcaagaa gcagattatg cgttctcata atgcgataca cccgctttgc attaaccaca
      481 ggcaggccgt cccgctccga ctcccgtcgc agcagcgccc acacacggcg ataaccatac
      541 gtcggcaggt cagccaccgc catgtttatc cgggacaata cggtggtatc gtcagaacgg
      601 ggctgccgtc tgcgatcctg tcagtcagat ggccggtgaa cccgaatgct caactgcgca
      661 cgcgacacgc caagacttct gcaaacgtcg cttattcgtc gttcccaggc aacgagggcg
      721 catgcgcaat ccattttttt gcccggccga actccacggc ctctttgagt atctcggctt
      781 ccatcgtctt tttgcccaga aggcgctgga gttcgcgaat ttgcttattg gcggcagcga
      841 gttcggaggc cggcaccact tcctcgcccg atgcaacagc cgtcagtgaa ccgtcttaat
      901 attgcttgcg ccatttgaat atctggtttg cattgatgcc atgcaggcgc gcgacatgag
      961 acacggtcat accgggctcc atagtctgct gaataatggc aattttttcc tgcggtgtac
     1021 gacgccgacg ccgctcaggt cctgataaca cttcaaccat cttatcgttc tgactggtat
     1081 taaacatagt tccaagacta cctcttattt taagagagtc gaagtgtctg gtgatctatg
     1141 gggccagtct agccctcttc caagtgtagg gagatatagt caaagtttgc ttctccgccg
     1201 atgagtccgc cctgcgtcgg atagtccgat tttttaccgg cgaagcacgg ctctcctaac
     1261 cctatctaaa gcgaataact tttttcaaca gggccgcgtg ccaatgcaag agcgataggc
     1321 gctctacgcg ccaatttgac cacttaaaac aggtaaattg aggggttgta atgtgttgat
     1381 gtaacaggct tttattttaa tgtcttggca tatgtataat ggtagtctag ccctcctttc
     1441 aacaaggagt actcatggaa acctacaatc atacatatcg gcaccacaac ttttcacata
     1501 aagacttaag tgatctcacc ttcaccgctt gcacattcat tcgcagcgac tttcgacgtg
     1561 ctaacttgcg tgatacgaca ttcgtcaact gcaagttcat tgaacagggt gatatcgaag
     1621 gctgccactt tgatgtcgca gatcttcgtg atgcaagttt ccaacaatgc caacttgcga
     1681 tggcaaactt cagtaatgcc aattgctacg gtatagagtt ccgtgcgtgt gatttaaaag
     1741 gtgccaactt ttcccgaaca aactttgccc atcaagtgag taatcgtatg tacttttgct
     1801 cagcatttat ttctggatgt aatctttcct atgccaatat ggagagggtt tgtttagaaa
     1861 aatgtgagtt gtttgaaaat cgctggatag gaacgaacct agcgggtgca tcactgaaag
     1921 agtcagactt aagtcgaggt gttttttccg aagatgtctg ggggcaattt agcctacagg
     1981 gtgccaattt atgccacgcc gaactcgacg gtttagatcc ccgcaaagtc gatacatcag
     2041 gtatcaaaat tgcagcctgg cagcaagaac tgattctcga agcactgggt attgttgttt
     2101 atcctgacta attgctttga tgtgtgattt taaacgctca aatttataaa acgaataata
     2161 ttgtcaacct tgcaataata gacataggga caaccatgta taaattttta ccctcaggcg
     2221 tcttcacaat agagccatat agcagtgggt cacttagtgg gcttaatttt tccattaaag
     2281 ataatattaa tattgctcag tataaaacat catatggtag cccatcttgg caagtaaaca
     2341 taaggctgcc atttataagg cactgttgca aaaatgtgga ggtgataggg gtactacgcc
     2401 ggaaccccag atttttccgt ccgttcaaat tatccacggt tgagcagctt gatcccgtcc
     2461 gcccgcagtt gaccaacagg ggaaatatgc ctgtacaaag tttgccgggt tatcccaagt
     2521 tcctggcata gcgtactgac ctttgtttct gattgtccca ttgatgccat tgccagtcgc
     2581 agtttgactg gcgtcatttt ataaggtcgg ccgccgttcc gcccacgggc tcttgctgat
     2641 gccagacctg cagtcgttct ttcagcaatc agttcgcgct caaactccgc cagtgcggca
     2701 aagataccga agacaagctt gccagcggcc gttgttgtgt cgatagtcgc cccgtgaccg
     2761 gtcagcactt tcagaccggt ccccctcgcg gtcaggtcgt gcactgtatt aatgagatga
     2821 cgaagatcac gaccgaggcg gtccagcttc catacgaccg agagcataaa aaatattggg
     2881 gcagcgtgag ctatgctgcc atcgctggtc ccttcccgtc actttgaaaa cccggatacc
     2941 agcagacaaa tttatcctgc agttcgttat tcaaaacggc ggtccgggtc tgtaacaaat
     3001 aatgagcccc ttttctgctc cactgcatct gctgcttttt ggccattcgt ctggcgatca
     3061 cttcattgat cgtggattcc acaaacgcgg ttgataccgg ctctccgtac cgacgcattt
     3121 cgccgtagtt tgggatcatc atcttattat tccggatgta ggtatacatt tcatccagat
     3181 gtttttgcag ggatttcagg ctaggatagc tgagttcagg gtcatcgcaa tacataacgc
     3241 aattatcaat atgttccagc gcagcgacaa cattgccatg ccagagatat cgtttaatgc
     3301 tttccagcag tgccagaact ttactccccg cctctggatc tgataccagc agtccccggg
     3361 catattgcat gagcaccttc agcctcatgg tgatatgaaa ccagtccagc acatgcgttg
     3421 actcagggta cataccgaac tggaggtccc tgagattatc cgcgccgtcg gacagaaaaa
     3481 atatctgctg gttggcctgc attccctgcg ctgaaaggtg ggtcatgagc ctgcgttccg
     3541 gatgacagtc atccttctga acaaaaccga agcggcgggt gtcagcaggc gcaccaacgg
     3601 aaaaagactt cccggcaata atttcaaaat tacgcttttt gtcatcccga tcgcgaacat
     3661 aaccaccatc tatacccacc acaagcggtt ttcctggcct gggcaggttc ccccagtcgc
     3721 gggggcagcc ggaaagaaaa cctgaatgag cttcagcctc agcgtcaaga cgctgcgcca
     3781 cctggcataa atgattcctc accgttgagg cattcaggct gtggcccacc ggcagaatat
     3841 ctttcagcag acgagtcgtc atttcatagg agatcatcga ggcccagcgg gtttcaatat
     3901 atttcagtgc cgggtgagaa taatcgccgg cccagtcgct gagcaaactg actgtctttg
     3961 tatcactctc ttcacaacgg caccggtaca cccgaagccc ggataccgga ataacgccaa
     4021 acagcgtccg gtaccgtatt ttctgtttgc ctttgattct gcgcgcagca aggcagtgag
     4081 ggcaccggat atggtgttga gtatattcgt ctgcctgcag ctggaccacc gactgctgga
     4141 ccgtattcag caacagcttg gattctgaca ccgataatcc gatgtcgttc cgggtctccc
     4201 ctgatttctg gattgtcatc agttcctccg ttcgactgga gcctgattca tcggtgatga
     4261 ctatctggag cgtcagttgc atcattgcct cctgctgtgg tggatgacta tctcccggct
     4321 actggttgtt catctggtga cgaagcttat aatatgtgga aacgccaagg cccgcatggt
     4381 cgcaggcgat cctgagcctg acccctctgc ttctgagtgc gtcaatttcc tggatctttt
     4441 tgatatcggc gccggggcgc cctgtttttt caccccgtgc ttttttcgcg gcgatgccgt
     4501 cggcctgcct ttccttcagt aaggctcgtt caaactggct gaatgccgac atcatgtgca
     4561 gctgcagttc ctgcatcgga ttgtttgtgc cggcgctgaa cgtcagctgc tctttcagaa
     4621 aaataagggt aacgccctgg tcacgaagcc gggtggtgac agcgcacata tccgacatat
     4681 tccggcaaag gcgatcgatg gaatgaacca gaagcgtatc accagggcgt aattgcccca
     4741 ggagttcctg taaccccggg cggtttgtgt ccttcgcgct ggcatgctca acacaaattt
     4801 tatcaggctg aaaacccgcc tttgacaatg cctcttcctg acgggccgta ttctgatcgg
     4861 ccgagcttac cctgatgtag gcatattgca cgcaaaacct ccggaaggtc tttaaacatc
     4921 atagagattt aaagaataat ccataaacac tgatttaggg aggtttatag acattttttc
     4981 ggggatctga gaggcatgct gaaaacgtat acctttatag acatcagtca gcgacgacgg
     5041 cccagccaga taacttcagg ctccagtacc ggagatgcaa ggtcgagcct gctcagttgc
     5101 cggttgattt tgtggcggtt gaacctgaca gcatccaggt cgtcagcaaa agggcggatc
     5161 tcgccgaccg ttgtttcatc atccgcatta acgatagctt caagaaacgc cagggtttta
     5221 tcgaactcat ccgcagctgt ggctccgggc ataccatggc cgcttataca aaacggcgct
     5281 ttcagcgtag agttttcata aatggcctca acacgaatgt catgaagcca gtgctcaaag
     5341 aaattgtatt cataggtaaa gcgatcgcca gcatcaaaag caaaatcatc aatcacgacc
     5401 cggaacggat tatcgacaaa accgatgcct ccttcgtagg agataccgta atctttgcca
     5461 taaatgtgaa attgatggag atggtcgtcc ccccagccct gcactatctg gaaaatgaag
     5521 tgaagcgcgg ccagtgacgt gtcggcagca attctcagcc gacgccagac catcggactg
     5581 actccatgaa ctgctatttt gatgacgtag atcttcatag tcagcaggta ttgttatgaa
     5641 atgatggtgg ttattttaac ccctgtagat ggcatcattc gatggctgcc ccgaggtttt
     5701 tcatgctctc catcttgtaa caatcaggca taccacggag cttgtttgct t
//
LOCUS       DADLDT020000404         5663 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.202, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000404 DADLDT020000000
VERSION     DADLDT020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.202"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            69..284
                     /locus_tag="JQH22_004575"
     CDS             69..284
                     /locus_tag="JQH22_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFB2208605.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            277..663
                     /locus_tag="JQH22_004576"
     CDS             277..663
                     /locus_tag="JQH22_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFB2208606.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            671..1357
                     /locus_tag="JQH22_004577"
     CDS             671..1357
                     /locus_tag="JQH22_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFB2208607.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="JQH22_004578"
     CDS             complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="JQH22_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFB2208608.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2040..3245
                     /gene="tet(B)"
                     /locus_tag="JQH22_004579"
     CDS             2040..3245
                     /gene="tet(B)"
                     /locus_tag="JQH22_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFB2208609.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="JQH22_004580"
     CDS             complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="JQH22_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFB2208610.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4084..4455
                     /locus_tag="JQH22_004581"
     CDS             4084..4455
                     /locus_tag="JQH22_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFB2208611.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4465..>5663)
                     /locus_tag="JQH22_004582"
     CDS             complement(4465..>5663)
                     /locus_tag="JQH22_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFB2208612.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1568 a         1044 c         1230 g         1821 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg accactatta agtcatgtag
       61 ctggttttat ttcaattgaa cgttttcaaa gtctagctac agaaggaaaa atgttatcgc
      121 tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga
      181 aagctcaaca agaagggcga gagtcaatat ttgattttta caaaattagt attgctcata
      241 ttactcgcga atattcattt aaaaaggaca aggataatgt ttgatgttca cgttgtttta
      301 gataatcaaa taggacaatt agcattacta ggaaaaacat taggtaataa aggtattgga
      361 ttggaagggg gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa
      421 caaggaaagg aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg
      481 acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga
      541 gtattggcgg agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg
      601 atattaataa cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag
      661 tgaacttgcg atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc
      721 gataggggcg gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg
      781 gcgagcttgg acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag
      841 cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca
      901 tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat
      961 ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg
     1021 tacttgctat gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca
     1081 acagcaatgg atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca
     1141 tgaaatggga attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga
     1201 ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta
     1261 tgaatctaaa gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa
     1321 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1381 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1441 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1501 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1561 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1621 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     1681 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     1741 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     1801 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     1861 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     1921 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1981 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
     2041 tgaatagttc gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc
     2101 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
     2161 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
     2221 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
     2281 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
     2341 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
     2401 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
     2461 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
     2521 tttttatcgc tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg
     2581 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt
     2641 cggtatacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
     2701 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
     2761 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
     2821 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
     2881 gatttattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     2941 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     3001 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     3061 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     3121 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     3181 tgctatcgat gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg
     3241 cttagttatt tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttacg ataaagaaga tttagcttca aataaaacct atctatttta
     3361 tttatctttc aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc
     3421 ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa
     3481 gtttttaagc acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac
     3541 aatggcgttt accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc
     3601 aatgataggc atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc
     3661 aagataatgt tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt
     3721 tgtgatgtag gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt
     3781 tttattttta aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc
     3841 atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag
     3901 tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt
     3961 aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa
     4021 gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat
     4081 aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta
     4141 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
     4201 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
     4261 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
     4321 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga
     4381 tttaagtaca tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg
     4441 gaggcaatgc actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
     4501 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
     4561 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
     4621 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
     4681 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
     4741 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
     4801 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
     4861 taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg
     4921 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
     4981 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
     5041 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
     5101 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
     5161 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
     5221 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
     5281 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
     5341 tcgcaatacc ataagtcgtt tttgctcacg aatatcagac cagtcaacaa gtacaatggg
     5401 catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt
     5461 gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc
     5521 tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt caagtaatgc
     5581 gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggt aatgactcca
     5641 acttactgat agtgttttat gtt
//
LOCUS       DADLDT020000405         1625 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.308, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000405 DADLDT020000000
VERSION     DADLDT020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.308"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..434
                     /locus_tag="JQH22_004583"
     CDS             <1..434
                     /locus_tag="JQH22_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208613.1"
                     /translation="TDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQ
                     GVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRR
                     TVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            617..1477
                     /locus_tag="JQH22_004584"
     CDS             617..1477
                     /locus_tag="JQH22_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor-resistant class A broad-spectrum
                     beta-lactamase TEM-40"
                     /protein_id="HFB2208614.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMISTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          437 a          356 c          414 g          418 t
ORIGIN      
        1 caacagaccg ggaagggctg gatttgctga ggatgaaggt ggaggaaggt gatgtcattc
       61 tggttaagaa gctcgaccgt cttggccgcg acactgccga tatgatccaa ctgataaagg
      121 aatttgacgc tcagggcgtg gcagtccggt tcattgatga cgggatcagt accgacggtg
      181 atatggggca aatggtggtc accatcctgt cggctgtggc acaggctgaa cgccggagga
      241 tcctagaacg cacgaatgag ggccgacagg aagcaaagct gaaaggaatc aaatttggcc
      301 gcaggcgtac cgtggacagg aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa
      361 cggaaattgc tcatcagctc agtattgccc gctccacggt ttataaaatt cttgaagacg
      421 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      481 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      541 aatacattca aatatgtatc cgctcatgag acaataaccc tggtaaatgc ttcaataata
      601 ttgaaaaagg aagagtatga gtattcaaca ttttcgtgtc gcccttattc ccttttttgc
      661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      781 tgagagtttt cgccccgaag aacgttttcc aatgataagc acttttaaag ttctgctatg
      841 tggtgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg ctgccaactt
     1021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1141 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1621 cccct
//
LOCUS       DADLDT020000406         1625 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.309, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000406 DADLDT020000000
VERSION     DADLDT020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.309"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            <1..434
                     /locus_tag="JQH22_004585"
     CDS             <1..434
                     /locus_tag="JQH22_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFB2208615.1"
                     /translation="TDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQ
                     GVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRR
                     TVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            617..1477
                     /locus_tag="JQH22_004586"
     CDS             617..1477
                     /locus_tag="JQH22_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HFB2208616.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          436 a          356 c          415 g          418 t
ORIGIN      
        1 caacagaccg ggaagggctg gatttgctga ggatgaaggt ggaggaaggt gatgtcattc
       61 tggttaagaa gctcgaccgt cttggccgcg acactgccga tatgatccaa ctgataaagg
      121 aatttgacgc tcagggcgtg gcagtccggt tcattgatga cgggatcagt accgacggtg
      181 atatggggca aatggtggtc accatcctgt cggctgtggc acaggctgaa cgccggagga
      241 tcctagaacg cacgaatgag ggccgacagg aagcaaagct gaaaggaatc aaatttggcc
      301 gcaggcgtac cgtggacagg aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa
      361 cggaaattgc tcatcagctc agtattgccc gctccacggt ttataaaatt cttgaagacg
      421 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      481 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      541 aatacattca aatatgtatc cgctcatgag acaataaccc tggtaaatgc ttcaataata
      601 ttgaaaaagg aagagtatga gtattcaaca ttttcgtgtc gcccttattc ccttttttgc
      661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      781 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      841 tggtgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg ctgccaactt
     1021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1141 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1621 cccct
//
LOCUS       DADLDT020000407          753 bp    DNA     linear   BCT 19-JUL-2024
DEFINITION  TPA_asm: Shigella flexneri 1c isolate MH150057
            SAMEA56758918-rid17776853.guided.364, whole genome shotgun
            sequence.
ACCESSION   DADLDT020000407 DADLDT020000000
VERSION     DADLDT020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA56758918
            Sequence Read Archive: ERR1811909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1c
  ORGANISM  Shigella flexneri 1c
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943558.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943558.2
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 01:38:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,587
            CDSs (total)                      :: 4,531
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 181 of 388
            Pseudo Genes (incomplete)         :: 105 of 388
            Pseudo Genes (internal stop)      :: 151 of 388
            Pseudo Genes (multiple problems)  :: 45 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella flexneri 1c"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA56758918-rid17776853.guided.364"
                     /isolate="MH150057"
                     /host="Homo sapiens"
                     /db_xref="taxon:1935181"
                     /geo_loc_name="Ireland"
                     /collection_date="2015"
     gene            57..530
                     /locus_tag="JQH22_004587"
     CDS             57..530
                     /locus_tag="JQH22_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFB2208617.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          219 a          160 c          182 g          192 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta acccaggatg agaaccttga
       61 aagtatcatt gatggctgcg aaagcgaaaa acggcgtgat tggttgcggt ccagacatac
      121 cctggtccgc gaaaggggag cagctacttt ttaaagcatt gacctacaat cagtggcttc
      181 tggtgggtcg caagacgttt gaatctatgg gcgcactccc caataggaaa tacgcggtcg
      241 ttacccgctc aggttggaca tcaaatgatg acaatgtagt tgtatttcag tcaatcgaag
      301 aggccatgga caggctagct gaattcaccg gtcacgttat agtgtctggt ggcggagaaa
      361 tttaccgaga aacattaccc atggcctcta cgctccactt atcgacgatc gacatcgagc
      421 cagaggggga tgttttcttc ccgagtattc caaatacctt cgaagttgtt tttgagcaac
      481 actttacttc aaacattaac tattgctatc aaatttggaa aaagggttaa caaagctatg
      541 caatcgacgg caaaaagctt cgttcgcttc gcgcactacg ccttttccgc gattgatagc
      601 gacgttatgt gaatattgaa atgagcattc caaagaaagg aagtagaaaa attatcgtgg
      661 gcgaaaatga gtttttgtgg ctcattaggt caaaactcac atattcgcag gacggcactg
      721 ttgcaaatag tcggtggtga taaacttatc atc
//
